Cloning of FSH‐β, LH‐β and glycoprotein hormone α subunits in pejerrey Odontesthes bonariensis (Valenciennes): expression profile and relationship with GnRH expression and plasma sex steroid levels in male fish
Identifieur interne : 001451 ( Istex/Corpus ); précédent : 001450; suivant : 001452Cloning of FSH‐β, LH‐β and glycoprotein hormone α subunits in pejerrey Odontesthes bonariensis (Valenciennes): expression profile and relationship with GnRH expression and plasma sex steroid levels in male fish
Auteurs : L. A. Miranda ; C. A. Strüssmann ; L. G. Guilgur ; P. H. Strobl-Mazzulla ; G. M. SomozaSource :
- Journal of Fish Biology [ 0022-1112 ] ; 2007-12.
English descriptors
Abstract
Three cDNAs encoding pejerrey Odontesthes bonariensis follicle stimulating hormone‐β (FSH‐β), luteinizing hormone‐β (LH‐β) and glycoprotein‐α (GPH‐α) subunits were cloned and characterized. Gene expression of these subunits was analysed by real‐time polymerase chain reaction (PCR) and compared with the brain gene expression of endogenous gonadotropin‐releasing hormones (GnRHs): Pacific salmon GnRH (GnRH‐III), pejerrey GnRH (GnRH‐I) and chicken GnRH‐II (GnRH‐II) and plasma sex steroid levels in adult males. The nucleotide sequences of the FSH‐β, LH‐β and GPH‐α subunits are 466, 558 and 677 base pairs long, encoding for mature peptides of 102, 118 and 98 amino acids respectively. Maturing males had high expression of FSH‐β and GPH‐α subunits, and intermediate levels of LH‐β when compared with running ripe and spent stages. These animals had the lowest plasma testosterone (T) and 11‐ketosterone (11‐KT) values as well as low expression of sGnRH, cGnRH‐II and pjGnRH. Running ripe males had the lowest expression of FSH‐β and the highest expression of LH‐β and GPH‐α subunits, and of the three GnRH genes. At this stage, the highest values of T and 11‐KT were observed. Spent males showed low expression of the three gonadotropin (GtH) subunits, sGnRH, pjGnRH and low levels of T. At this stage, 11‐KT levels and cGnRH‐II expression showed a tendency to decrease but the values were not statistically significant (P < 0·05) to running ripe stage. The present results would suggest that T and 11‐KT modulate the expression of the FSH subunits. The expression of the anterior brain GnRH variants, sGnRH and pjGnRH is correlated with LH‐β expression and reinforce the importance of the forebrain GnRH variants on the regulation of pituitary function.
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DOI: 10.1111/j.1095-8649.2007.01621.x
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A." last="Miranda">L. A. Miranda</name><affiliation><mods:affiliation>* Laboratorio de Ictiofisiología y Acuicultura, Instituto de Investigaciones Biotecnológicas‐Instituto Tecnológico de Chascomús (B7130IWA), Chascomús, provincia de Buenos Aires, Argentina and ‡ Tokyo University of Marine Science and Technology, Department of Marine Biosciences, 4‐5‐7 Konan, Minato‐Ku, Tokyo, Japan</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: lmiranda@intech.gov.ar</mods:affiliation></affiliation></author><author><name sortKey="Strussmann, C A" sort="Strussmann, C A" uniqKey="Strussmann C" first="C. A." last="Strüssmann">C. A. Strüssmann</name><affiliation><mods:affiliation>* Laboratorio de Ictiofisiología y Acuicultura, Instituto de Investigaciones Biotecnológicas‐Instituto Tecnológico de Chascomús (B7130IWA), Chascomús, provincia de Buenos Aires, Argentina and ‡ Tokyo University of Marine Science and Technology, Department of Marine Biosciences, 4‐5‐7 Konan, Minato‐Ku, Tokyo, Japan</mods:affiliation></affiliation></author><author><name sortKey="Guilgur, L G" sort="Guilgur, L G" uniqKey="Guilgur L" first="L. G." last="Guilgur">L. G. Guilgur</name><affiliation><mods:affiliation>* Laboratorio de Ictiofisiología y Acuicultura, Instituto de Investigaciones Biotecnológicas‐Instituto Tecnológico de Chascomús (B7130IWA), Chascomús, provincia de Buenos Aires, Argentina and ‡ Tokyo University of Marine Science and Technology, Department of Marine Biosciences, 4‐5‐7 Konan, Minato‐Ku, Tokyo, Japan</mods:affiliation></affiliation></author><author><name sortKey="Strobl Azzulla, P H" sort="Strobl Azzulla, P H" uniqKey="Strobl Azzulla P" first="P. H." last="Strobl-Mazzulla">P. H. Strobl-Mazzulla</name><affiliation><mods:affiliation>* Laboratorio de Ictiofisiología y Acuicultura, Instituto de Investigaciones Biotecnológicas‐Instituto Tecnológico de Chascomús (B7130IWA), Chascomús, provincia de Buenos Aires, Argentina and ‡ Tokyo University of Marine Science and Technology, Department of Marine Biosciences, 4‐5‐7 Konan, Minato‐Ku, Tokyo, Japan</mods:affiliation></affiliation></author><author><name sortKey="Somoza, G M" sort="Somoza, G M" uniqKey="Somoza G" first="G. M." last="Somoza">G. M. Somoza</name><affiliation><mods:affiliation>* Laboratorio de Ictiofisiología y Acuicultura, Instituto de Investigaciones Biotecnológicas‐Instituto Tecnológico de Chascomús (B7130IWA), Chascomús, provincia de Buenos Aires, Argentina and ‡ Tokyo University of Marine Science and Technology, Department of Marine Biosciences, 4‐5‐7 Konan, Minato‐Ku, Tokyo, Japan</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Fish Biology</title><idno type="ISSN">0022-1112</idno><idno type="eISSN">1095-8649</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2007-12">2007-12</date><biblScope unit="volume">71</biblScope><biblScope unit="issue">6</biblScope><biblScope unit="page" from="1571">1571</biblScope><biblScope unit="page" to="1589">1589</biblScope></imprint><idno type="ISSN">0022-1112</idno></series><idno type="istex">88FB76244380B30E8EA3AD189CA8F552D757180F</idno><idno type="DOI">10.1111/j.1095-8649.2007.01621.x</idno><idno type="ArticleID">JFB1621</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0022-1112</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>gonadotropins</term><term>gonadotropin‐releasing hormones</term><term>pejerrey</term><term>sex steroids</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Three cDNAs encoding pejerrey Odontesthes bonariensis follicle stimulating hormone‐β (FSH‐β), luteinizing hormone‐β (LH‐β) and glycoprotein‐α (GPH‐α) subunits were cloned and characterized. Gene expression of these subunits was analysed by real‐time polymerase chain reaction (PCR) and compared with the brain gene expression of endogenous gonadotropin‐releasing hormones (GnRHs): Pacific salmon GnRH (GnRH‐III), pejerrey GnRH (GnRH‐I) and chicken GnRH‐II (GnRH‐II) and plasma sex steroid levels in adult males. The nucleotide sequences of the FSH‐β, LH‐β and GPH‐α subunits are 466, 558 and 677 base pairs long, encoding for mature peptides of 102, 118 and 98 amino acids respectively. Maturing males had high expression of FSH‐β and GPH‐α subunits, and intermediate levels of LH‐β when compared with running ripe and spent stages. These animals had the lowest plasma testosterone (T) and 11‐ketosterone (11‐KT) values as well as low expression of sGnRH, cGnRH‐II and pjGnRH. Running ripe males had the lowest expression of FSH‐β and the highest expression of LH‐β and GPH‐α subunits, and of the three GnRH genes. At this stage, the highest values of T and 11‐KT were observed. Spent males showed low expression of the three gonadotropin (GtH) subunits, sGnRH, pjGnRH and low levels of T. At this stage, 11‐KT levels and cGnRH‐II expression showed a tendency to decrease but the values were not statistically significant (P < 0·05) to running ripe stage. The present results would suggest that T and 11‐KT modulate the expression of the FSH subunits. The expression of the anterior brain GnRH variants, sGnRH and pjGnRH is correlated with LH‐β expression and reinforce the importance of the forebrain GnRH variants on the regulation of pituitary function.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>L. A. Miranda</name><affiliations><json:string>* Laboratorio de Ictiofisiología y Acuicultura, Instituto de Investigaciones Biotecnológicas‐Instituto Tecnológico de Chascomús (B7130IWA), Chascomús, provincia de Buenos Aires, Argentina and ‡ Tokyo University of Marine Science and Technology, Department of Marine Biosciences, 4‐5‐7 Konan, Minato‐Ku, Tokyo, Japan</json:string><json:string>E-mail: lmiranda@intech.gov.ar</json:string></affiliations></json:item><json:item><name>C. A. Strüssmann</name><affiliations><json:string>* Laboratorio de Ictiofisiología y Acuicultura, Instituto de Investigaciones Biotecnológicas‐Instituto Tecnológico de Chascomús (B7130IWA), Chascomús, provincia de Buenos Aires, Argentina and ‡ Tokyo University of Marine Science and Technology, Department of Marine Biosciences, 4‐5‐7 Konan, Minato‐Ku, Tokyo, Japan</json:string></affiliations></json:item><json:item><name>L. G. Guilgur</name><affiliations><json:string>* Laboratorio de Ictiofisiología y Acuicultura, Instituto de Investigaciones Biotecnológicas‐Instituto Tecnológico de Chascomús (B7130IWA), Chascomús, provincia de Buenos Aires, Argentina and ‡ Tokyo University of Marine Science and Technology, Department of Marine Biosciences, 4‐5‐7 Konan, Minato‐Ku, Tokyo, Japan</json:string></affiliations></json:item><json:item><name>P. H. Strobl‐Mazzulla</name><affiliations><json:string>* Laboratorio de Ictiofisiología y Acuicultura, Instituto de Investigaciones Biotecnológicas‐Instituto Tecnológico de Chascomús (B7130IWA), Chascomús, provincia de Buenos Aires, Argentina and ‡ Tokyo University of Marine Science and Technology, Department of Marine Biosciences, 4‐5‐7 Konan, Minato‐Ku, Tokyo, Japan</json:string></affiliations></json:item><json:item><name>G. M. Somoza</name><affiliations><json:string>* Laboratorio de Ictiofisiología y Acuicultura, Instituto de Investigaciones Biotecnológicas‐Instituto Tecnológico de Chascomús (B7130IWA), Chascomús, provincia de Buenos Aires, Argentina and ‡ Tokyo University of Marine Science and Technology, Department of Marine Biosciences, 4‐5‐7 Konan, Minato‐Ku, Tokyo, Japan</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>gonadotropins</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>gonadotropin‐releasing hormones</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>pejerrey</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>sex steroids</value></json:item></subject><articleId><json:string>JFB1621</json:string></articleId><language><json:string>eng</json:string></language><originalGenre><json:string>article</json:string></originalGenre><abstract>Three cDNAs encoding pejerrey Odontesthes bonariensis follicle stimulating hormone‐β (FSH‐β), luteinizing hormone‐β (LH‐β) and glycoprotein‐α (GPH‐α) subunits were cloned and characterized. Gene expression of these subunits was analysed by real‐time polymerase chain reaction (PCR) and compared with the brain gene expression of endogenous gonadotropin‐releasing hormones (GnRHs): Pacific salmon GnRH (GnRH‐III), pejerrey GnRH (GnRH‐I) and chicken GnRH‐II (GnRH‐II) and plasma sex steroid levels in adult males. The nucleotide sequences of the FSH‐β, LH‐β and GPH‐α subunits are 466, 558 and 677 base pairs long, encoding for mature peptides of 102, 118 and 98 amino acids respectively. Maturing males had high expression of FSH‐β and GPH‐α subunits, and intermediate levels of LH‐β when compared with running ripe and spent stages. These animals had the lowest plasma testosterone (T) and 11‐ketosterone (11‐KT) values as well as low expression of sGnRH, cGnRH‐II and pjGnRH. Running ripe males had the lowest expression of FSH‐β and the highest expression of LH‐β and GPH‐α subunits, and of the three GnRH genes. At this stage, the highest values of T and 11‐KT were observed. Spent males showed low expression of the three gonadotropin (GtH) subunits, sGnRH, pjGnRH and low levels of T. At this stage, 11‐KT levels and cGnRH‐II expression showed a tendency to decrease but the values were not statistically significant (P > 0·05) to running ripe stage. The present results would suggest that T and 11‐KT modulate the expression of the FSH subunits. The expression of the anterior brain GnRH variants, sGnRH and pjGnRH is correlated with LH‐β expression and reinforce the importance of the forebrain GnRH variants on the regulation of pituitary function.</abstract><qualityIndicators><score>8</score><pdfVersion>1.3</pdfVersion><pdfPageSize>488 x 703 pts</pdfPageSize><refBibsNative>false</refBibsNative><abstractCharCount>1756</abstractCharCount><pdfWordCount>7587</pdfWordCount><pdfCharCount>46520</pdfCharCount><pdfPageCount>19</pdfPageCount><abstractWordCount>268</abstractWordCount></qualityIndicators><title>Cloning of FSH‐β, LH‐β and glycoprotein hormone α subunits in pejerrey Odontesthes bonariensis (Valenciennes): expression profile and relationship with GnRH expression and plasma sex steroid levels in male fish</title><genre><json:string>article</json:string></genre><host><volume>71</volume><publisherId><json:string>JFB</json:string></publisherId><pages><total>19</total><last>1589</last><first>1571</first></pages><issn><json:string>0022-1112</json:string></issn><issue>6</issue><genre><json:string>journal</json:string></genre><language><json:string>unknown</json:string></language><eissn><json:string>1095-8649</json:string></eissn><title>Journal of Fish Biology</title><doi><json:string>10.1111/(ISSN)1095-8649</json:string></doi></host><categories><wos><json:string>science</json:string><json:string>marine & freshwater biology</json:string><json:string>fisheries</json:string></wos><scienceMetrix><json:string>applied sciences</json:string><json:string>agriculture, fisheries & forestry</json:string><json:string>fisheries</json:string></scienceMetrix></categories><publicationDate>2007</publicationDate><copyrightDate>2007</copyrightDate><doi><json:string>10.1111/j.1095-8649.2007.01621.x</json:string></doi><id>88FB76244380B30E8EA3AD189CA8F552D757180F</id><score>0.015932105</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/document/88FB76244380B30E8EA3AD189CA8F552D757180F/fulltext/pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/document/88FB76244380B30E8EA3AD189CA8F552D757180F/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/88FB76244380B30E8EA3AD189CA8F552D757180F/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Cloning of FSH‐β, LH‐β and glycoprotein hormone α subunits in pejerrey Odontesthes bonariensis (Valenciennes): expression profile and relationship with GnRH expression and plasma sex steroid levels in male fish</title><respStmt><resp>Références bibliographiques récupérées via GROBID</resp><name resp="ISTEX-API">ISTEX-API (INIST-CNRS)</name></respStmt><respStmt><resp>Références bibliographiques récupérées via GROBID</resp><name resp="ISTEX-API">ISTEX-API (INIST-CNRS)</name></respStmt></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><availability><p>2007 The Fisheries Society of the British Isles</p></availability><date>2007</date></publicationStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Cloning of FSH‐β, LH‐β and glycoprotein hormone α subunits in pejerrey Odontesthes bonariensis (Valenciennes): expression profile and relationship with GnRH expression and plasma sex steroid levels in male fish</title><author xml:id="author-1"><persName><forename type="first">L. A.</forename><surname>Miranda</surname></persName><email>lmiranda@intech.gov.ar</email><affiliation>* Laboratorio de Ictiofisiología y Acuicultura, Instituto de Investigaciones Biotecnológicas‐Instituto Tecnológico de Chascomús (B7130IWA), Chascomús, provincia de Buenos Aires, Argentina and ‡ Tokyo University of Marine Science and Technology, Department of Marine Biosciences, 4‐5‐7 Konan, Minato‐Ku, Tokyo, Japan</affiliation></author><author xml:id="author-2"><persName><forename type="first">C. A.</forename><surname>Strüssmann</surname></persName><affiliation>* Laboratorio de Ictiofisiología y Acuicultura, Instituto de Investigaciones Biotecnológicas‐Instituto Tecnológico de Chascomús (B7130IWA), Chascomús, provincia de Buenos Aires, Argentina and ‡ Tokyo University of Marine Science and Technology, Department of Marine Biosciences, 4‐5‐7 Konan, Minato‐Ku, Tokyo, Japan</affiliation></author><author xml:id="author-3"><persName><forename type="first">L. G.</forename><surname>Guilgur</surname></persName><affiliation>* Laboratorio de Ictiofisiología y Acuicultura, Instituto de Investigaciones Biotecnológicas‐Instituto Tecnológico de Chascomús (B7130IWA), Chascomús, provincia de Buenos Aires, Argentina and ‡ Tokyo University of Marine Science and Technology, Department of Marine Biosciences, 4‐5‐7 Konan, Minato‐Ku, Tokyo, Japan</affiliation></author><author xml:id="author-4"><persName><forename type="first">P. H.</forename><surname>Strobl‐Mazzulla</surname></persName><affiliation>* Laboratorio de Ictiofisiología y Acuicultura, Instituto de Investigaciones Biotecnológicas‐Instituto Tecnológico de Chascomús (B7130IWA), Chascomús, provincia de Buenos Aires, Argentina and ‡ Tokyo University of Marine Science and Technology, Department of Marine Biosciences, 4‐5‐7 Konan, Minato‐Ku, Tokyo, Japan</affiliation></author><author xml:id="author-5"><persName><forename type="first">G. M.</forename><surname>Somoza</surname></persName><affiliation>* Laboratorio de Ictiofisiología y Acuicultura, Instituto de Investigaciones Biotecnológicas‐Instituto Tecnológico de Chascomús (B7130IWA), Chascomús, provincia de Buenos Aires, Argentina and ‡ Tokyo University of Marine Science and Technology, Department of Marine Biosciences, 4‐5‐7 Konan, Minato‐Ku, Tokyo, Japan</affiliation></author></analytic><monogr><title level="j">Journal of Fish Biology</title><idno type="pISSN">0022-1112</idno><idno type="eISSN">1095-8649</idno><idno type="DOI">10.1111/(ISSN)1095-8649</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2007-12"></date><biblScope unit="volume">71</biblScope><biblScope unit="issue">6</biblScope><biblScope unit="page" from="1571">1571</biblScope><biblScope unit="page" to="1589">1589</biblScope></imprint></monogr><idno type="istex">88FB76244380B30E8EA3AD189CA8F552D757180F</idno><idno type="DOI">10.1111/j.1095-8649.2007.01621.x</idno><idno type="ArticleID">JFB1621</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2007</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Three cDNAs encoding pejerrey Odontesthes bonariensis follicle stimulating hormone‐β (FSH‐β), luteinizing hormone‐β (LH‐β) and glycoprotein‐α (GPH‐α) subunits were cloned and characterized. Gene expression of these subunits was analysed by real‐time polymerase chain reaction (PCR) and compared with the brain gene expression of endogenous gonadotropin‐releasing hormones (GnRHs): Pacific salmon GnRH (GnRH‐III), pejerrey GnRH (GnRH‐I) and chicken GnRH‐II (GnRH‐II) and plasma sex steroid levels in adult males. The nucleotide sequences of the FSH‐β, LH‐β and GPH‐α subunits are 466, 558 and 677 base pairs long, encoding for mature peptides of 102, 118 and 98 amino acids respectively. Maturing males had high expression of FSH‐β and GPH‐α subunits, and intermediate levels of LH‐β when compared with running ripe and spent stages. These animals had the lowest plasma testosterone (T) and 11‐ketosterone (11‐KT) values as well as low expression of sGnRH, cGnRH‐II and pjGnRH. Running ripe males had the lowest expression of FSH‐β and the highest expression of LH‐β and GPH‐α subunits, and of the three GnRH genes. At this stage, the highest values of T and 11‐KT were observed. Spent males showed low expression of the three gonadotropin (GtH) subunits, sGnRH, pjGnRH and low levels of T. At this stage, 11‐KT levels and cGnRH‐II expression showed a tendency to decrease but the values were not statistically significant (P < 0·05) to running ripe stage. The present results would suggest that T and 11‐KT modulate the expression of the FSH subunits. The expression of the anterior brain GnRH variants, sGnRH and pjGnRH is correlated with LH‐β expression and reinforce the importance of the forebrain GnRH variants on the regulation of pituitary function.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>keywords</head><item><term>gonadotropins</term></item><item><term>gonadotropin‐releasing hormones</term></item><item><term>pejerrey</term></item><item><term>sex steroids</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2007-12">Published</change><change xml:id="refBibs-istex" who="#ISTEX-API" when="2016-12-13">References added</change><change xml:id="refBibs-istex" who="#ISTEX-API" when="2017-02-8">References added</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/88FB76244380B30E8EA3AD189CA8F552D757180F/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>Blackwell Publishing Ltd</publisherName><publisherLoc>Oxford, UK</publisherLoc></publisherInfo><doi origin="wiley" registered="yes">10.1111/(ISSN)1095-8649</doi><issn type="print">0022-1112</issn><issn type="electronic">1095-8649</issn><idGroup><id type="product" value="JFB"></id><id type="publisherDivision" value="ST"></id></idGroup><titleGroup><title type="main" sort="JOURNAL OF FISH BIOLOGY">Journal of Fish Biology</title></titleGroup></publicationMeta><publicationMeta level="part" position="12006"><doi origin="wiley">10.1111/jfb.2007.71.issue-6</doi><numberingGroup><numbering type="journalVolume" number="71">71</numbering><numbering type="journalIssue" number="6">6</numbering></numberingGroup><coverDate startDate="2007-12">December 2007</coverDate></publicationMeta><publicationMeta level="unit" type="article" position="1" status="forIssue"><doi origin="wiley">10.1111/j.1095-8649.2007.01621.x</doi><idGroup><id type="unit" value="JFB1621"></id></idGroup><countGroup><count type="pageTotal" number="19"></count></countGroup><titleGroup><title type="tocHeading1">REGULAR PAPERS</title></titleGroup><copyright>2007 The Fisheries Society of the British Isles</copyright><eventGroup><event type="firstOnline" date="2007-12-07"></event><event type="publishedOnlineFinalForm" date="2007-12-07"></event><event type="xmlConverted" agent="Converter:BPG_TO_WML3G version:2.3.2 mode:FullText source:HeaderRef result:HeaderRef" date="2010-02-28"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:3.8.8" date="2014-01-30"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-10-30"></event></eventGroup><numberingGroup><numbering type="pageFirst" number="1571">1571</numbering><numbering type="pageLast" number="1589">1589</numbering></numberingGroup><correspondenceTo> †Tel.: +54 2241 424048; fax: +54 2241 424048; email: <email>lmiranda@intech.gov.ar</email></correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:JFB.JFB1621.pdf"></link></linkGroup></publicationMeta><contentMeta><unparsedEditorialHistory>(Received 25 September 2006, Accepted 19 June 2007)</unparsedEditorialHistory><countGroup><count type="figureTotal" number="8"></count><count type="tableTotal" number="2"></count><count type="formulaTotal" number="0"></count><count type="referenceTotal" number="69"></count><count type="wordTotal" number="0"></count><count type="linksPubMed" number="0"></count><count type="linksCrossRef" number="0"></count></countGroup><titleGroup><title type="main">Cloning of FSH‐β, LH‐β and glycoprotein hormone α subunits in pejerrey <i>Odontesthes bonariensis</i> (Valenciennes): expression profile and relationship with GnRH expression and plasma sex steroid levels in male fish</title></titleGroup><creators><creator creatorRole="author" xml:id="cr1" affiliationRef="#aff-1-1" corresponding="yes"><personName><givenNames>L. A.</givenNames><familyName>Miranda</familyName></personName></creator><creator creatorRole="author" xml:id="cr2" affiliationRef="#aff-1-1"><personName><givenNames>C. A.</givenNames><familyName>Strüssmann</familyName></personName></creator><creator creatorRole="author" xml:id="cr3" affiliationRef="#aff-1-1"><personName><givenNames>L. G.</givenNames><familyName>Guilgur</familyName></personName></creator><creator creatorRole="author" xml:id="cr4" affiliationRef="#aff-1-1"><personName><givenNames>P. H.</givenNames><familyName>Strobl‐Mazzulla</familyName></personName></creator><creator creatorRole="author" xml:id="cr5" affiliationRef="#aff-1-1"><personName><givenNames>G. M.</givenNames><familyName>Somoza</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="aff-1-1"><unparsedAffiliation> * <i>Laboratorio de Ictiofisiología y Acuicultura, Instituto de Investigaciones Biotecnológicas‐Instituto Tecnológico de Chascomús (B7130IWA), Chascomús, provincia de Buenos Aires, Argentina</i><i> and </i> ‡ <i>Tokyo University of Marine Science and Technology, Department of Marine Biosciences, 4‐5‐7 Konan, Minato‐Ku, Tokyo, Japan</i></unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en"><keyword xml:id="k1">gonadotropins</keyword><keyword xml:id="k2">gonadotropin‐releasing hormones</keyword><keyword xml:id="k3">pejerrey</keyword><keyword xml:id="k4">sex steroids</keyword></keywordGroup><abstractGroup><abstract type="main" xml:lang="en"><p>Three cDNAs encoding pejerrey <i>Odontesthes bonariensis</i> follicle stimulating hormone‐β (FSH‐β), luteinizing hormone‐β (LH‐β) and glycoprotein‐α (GPH‐α) subunits were cloned and characterized. Gene expression of these subunits was analysed by real‐time polymerase chain reaction (PCR) and compared with the brain gene expression of endogenous gonadotropin‐releasing hormones (GnRHs): Pacific salmon GnRH (GnRH‐III), pejerrey GnRH (GnRH‐I) and chicken GnRH‐II (GnRH‐II) and plasma sex steroid levels in adult males. The nucleotide sequences of the FSH‐β, LH‐β and GPH‐α subunits are 466, 558 and 677 base pairs long, encoding for mature peptides of 102, 118 and 98 amino acids respectively. Maturing males had high expression of FSH‐β and GPH‐α subunits, and intermediate levels of LH‐β when compared with running ripe and spent stages. These animals had the lowest plasma testosterone (T) and 11‐ketosterone (11‐KT) values as well as low expression of sGnRH, cGnRH‐II and pjGnRH. Running ripe males had the lowest expression of FSH‐β and the highest expression of LH‐β and GPH‐α subunits, and of the three GnRH genes. At this stage, the highest values of T and 11‐KT were observed. Spent males showed low expression of the three gonadotropin (GtH) subunits, sGnRH, pjGnRH and low levels of T. At this stage, 11‐KT levels and cGnRH‐II expression showed a tendency to decrease but the values were not statistically significant (<i>P</i> < 0·05) to running ripe stage. The present results would suggest that T and 11‐KT modulate the expression of the FSH subunits. The expression of the anterior brain GnRH variants, sGnRH and pjGnRH is correlated with LH‐β expression and reinforce the importance of the forebrain GnRH variants on the regulation of pituitary function.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Cloning of FSH‐β, LH‐β and glycoprotein hormone α subunits in pejerrey Odontesthes bonariensis (Valenciennes): expression profile and relationship with GnRH expression and plasma sex steroid levels in male fish</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Cloning of FSH‐β, LH‐β and glycoprotein hormone α subunits in pejerrey Odontesthes bonariensis (Valenciennes): expression profile and relationship with GnRH expression and plasma sex steroid levels in male fish</title></titleInfo><name type="personal"><namePart type="given">L. A.</namePart><namePart type="family">Miranda</namePart><affiliation>* Laboratorio de Ictiofisiología y Acuicultura, Instituto de Investigaciones Biotecnológicas‐Instituto Tecnológico de Chascomús (B7130IWA), Chascomús, provincia de Buenos Aires, Argentina and ‡ Tokyo University of Marine Science and Technology, Department of Marine Biosciences, 4‐5‐7 Konan, Minato‐Ku, Tokyo, Japan</affiliation><affiliation>E-mail: lmiranda@intech.gov.ar</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">C. A.</namePart><namePart type="family">Strüssmann</namePart><affiliation>* Laboratorio de Ictiofisiología y Acuicultura, Instituto de Investigaciones Biotecnológicas‐Instituto Tecnológico de Chascomús (B7130IWA), Chascomús, provincia de Buenos Aires, Argentina and ‡ Tokyo University of Marine Science and Technology, Department of Marine Biosciences, 4‐5‐7 Konan, Minato‐Ku, Tokyo, Japan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">L. G.</namePart><namePart type="family">Guilgur</namePart><affiliation>* Laboratorio de Ictiofisiología y Acuicultura, Instituto de Investigaciones Biotecnológicas‐Instituto Tecnológico de Chascomús (B7130IWA), Chascomús, provincia de Buenos Aires, Argentina and ‡ Tokyo University of Marine Science and Technology, Department of Marine Biosciences, 4‐5‐7 Konan, Minato‐Ku, Tokyo, Japan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">P. H.</namePart><namePart type="family">Strobl‐Mazzulla</namePart><affiliation>* Laboratorio de Ictiofisiología y Acuicultura, Instituto de Investigaciones Biotecnológicas‐Instituto Tecnológico de Chascomús (B7130IWA), Chascomús, provincia de Buenos Aires, Argentina and ‡ Tokyo University of Marine Science and Technology, Department of Marine Biosciences, 4‐5‐7 Konan, Minato‐Ku, Tokyo, Japan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">G. M.</namePart><namePart type="family">Somoza</namePart><affiliation>* Laboratorio de Ictiofisiología y Acuicultura, Instituto de Investigaciones Biotecnológicas‐Instituto Tecnológico de Chascomús (B7130IWA), Chascomús, provincia de Buenos Aires, Argentina and ‡ Tokyo University of Marine Science and Technology, Department of Marine Biosciences, 4‐5‐7 Konan, Minato‐Ku, Tokyo, Japan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Blackwell Publishing Ltd</publisher><place><placeTerm type="text">Oxford, UK</placeTerm></place><dateIssued encoding="w3cdtf">2007-12</dateIssued><edition>(Received 25 September 2006, Accepted 19 June 2007)</edition><copyrightDate encoding="w3cdtf">2007</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">8</extent><extent unit="tables">2</extent><extent unit="references">69</extent></physicalDescription><abstract lang="en">Three cDNAs encoding pejerrey Odontesthes bonariensis follicle stimulating hormone‐β (FSH‐β), luteinizing hormone‐β (LH‐β) and glycoprotein‐α (GPH‐α) subunits were cloned and characterized. Gene expression of these subunits was analysed by real‐time polymerase chain reaction (PCR) and compared with the brain gene expression of endogenous gonadotropin‐releasing hormones (GnRHs): Pacific salmon GnRH (GnRH‐III), pejerrey GnRH (GnRH‐I) and chicken GnRH‐II (GnRH‐II) and plasma sex steroid levels in adult males. The nucleotide sequences of the FSH‐β, LH‐β and GPH‐α subunits are 466, 558 and 677 base pairs long, encoding for mature peptides of 102, 118 and 98 amino acids respectively. Maturing males had high expression of FSH‐β and GPH‐α subunits, and intermediate levels of LH‐β when compared with running ripe and spent stages. These animals had the lowest plasma testosterone (T) and 11‐ketosterone (11‐KT) values as well as low expression of sGnRH, cGnRH‐II and pjGnRH. Running ripe males had the lowest expression of FSH‐β and the highest expression of LH‐β and GPH‐α subunits, and of the three GnRH genes. At this stage, the highest values of T and 11‐KT were observed. Spent males showed low expression of the three gonadotropin (GtH) subunits, sGnRH, pjGnRH and low levels of T. At this stage, 11‐KT levels and cGnRH‐II expression showed a tendency to decrease but the values were not statistically significant (P < 0·05) to running ripe stage. The present results would suggest that T and 11‐KT modulate the expression of the FSH subunits. The expression of the anterior brain GnRH variants, sGnRH and pjGnRH is correlated with LH‐β expression and reinforce the importance of the forebrain GnRH variants on the regulation of pituitary function.</abstract><subject lang="en"><genre>keywords</genre><topic>gonadotropins</topic><topic>gonadotropin‐releasing hormones</topic><topic>pejerrey</topic><topic>sex steroids</topic></subject><relatedItem type="host"><titleInfo><title>Journal of Fish Biology</title></titleInfo><genre type="journal">journal</genre><identifier type="ISSN">0022-1112</identifier><identifier type="eISSN">1095-8649</identifier><identifier type="DOI">10.1111/(ISSN)1095-8649</identifier><identifier type="PublisherID">JFB</identifier><part><date>2007</date><detail type="volume"><caption>vol.</caption><number>71</number></detail><detail type="issue"><caption>no.</caption><number>6</number></detail><extent unit="pages"><start>1571</start><end>1589</end><total>19</total></extent></part></relatedItem><identifier type="istex">88FB76244380B30E8EA3AD189CA8F552D757180F</identifier><identifier type="DOI">10.1111/j.1095-8649.2007.01621.x</identifier><identifier type="ArticleID">JFB1621</identifier><accessCondition type="use and reproduction" contentType="copyright">2007 The Fisheries Society of the British Isles</accessCondition><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>Blackwell Publishing Ltd</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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