Variable number of tandem repeats in the growth hormone gene of Sparus aurata: association with growth and effect on gene transcription
Identifieur interne : 001449 ( Istex/Corpus ); précédent : 001448; suivant : 001450Variable number of tandem repeats in the growth hormone gene of Sparus aurata: association with growth and effect on gene transcription
Auteurs : R. Almuly ; Y. Poleg-Danin ; Y. Kashi ; S. Gorshkov ; G. Gorshkova ; A. Dyman ; B. Cavari ; B. Rapoport ; M. Soller ; B. FunkensteinSource :
- Journal of Fish Biology [ 0022-1112 ] ; 2004-12.
Abstract
The GH gene of Sparus aurata(saGH) contains variable number of tandem repeats (VNTR). The hyper‐variable minisatellites in the first and third introns segregate in a Mendelian manner and exhibit numerous alleles. Analysis by PCR and sequencing of the two introns in several wild Sparidae species revealed comparable minisatellites with some variations. ‘Zoo blot’ with the first intron unit as a probe showed this sequence to be characteristic of several families from the Perciformes order. Unexpectedly, a similar minisatellite was found in the first intron of the GH gene in flounder, which belongs to a different order. Transfection of constructs containing a reporter gene and first intron of different length to four cell lines resulted in an inhibitory effect of the longer intron relative to the short intron. A (CA)n microsatellite (saGHpCA) is found in the GH promoter. A similar repeat at the same location is present in GH promoters of several other fish species. High variability (11 alleles) of the saGHpCA was found in a hatchery population. Full‐sib family genotyping showed a Mendelian inheritance of these alleles. A significant association was found between allele distribution and body mass in large and average size fishes from a hatchery population. The intron minisatellites may serve as markers for hybrid population and parental assignment. Its presence in families and orders of the higher teleosts may help solving classification uncertainties. Their conservation and inhibitory effect suggest a biological role. The saGHpCA is correlated with growth and may be a good candidate for predicting growth performance.
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DOI: 10.1111/j.0022-1112.2004.00559ab.x
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Funkenstein</name><affiliation><mods:affiliation>(National Institute of Oceanography, Israel Oceanographic & Limnological Research, Haifa, Israel;</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Fish Biology</title><idno type="ISSN">0022-1112</idno><idno type="eISSN">1095-8649</idno><imprint><publisher>Blackwell Science Ltd</publisher><pubPlace>Oxford, UK; Malden, USA</pubPlace><date type="published" when="2004-12">2004-12</date><biblScope unit="volume">65</biblScope><biblScope unit="supplement">s1</biblScope><biblScope unit="page" from="326">326</biblScope><biblScope unit="page" to="326">326</biblScope></imprint><idno type="ISSN">0022-1112</idno></series><idno type="istex">F17ECAC340969B2F7E8F5D869A6771078055BC8F</idno><idno type="DOI">10.1111/j.0022-1112.2004.00559ab.x</idno><idno type="ArticleID">JFB559AB</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0022-1112</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The GH gene of Sparus aurata(saGH) contains variable number of tandem repeats (VNTR). The hyper‐variable minisatellites in the first and third introns segregate in a Mendelian manner and exhibit numerous alleles. Analysis by PCR and sequencing of the two introns in several wild Sparidae species revealed comparable minisatellites with some variations. ‘Zoo blot’ with the first intron unit as a probe showed this sequence to be characteristic of several families from the Perciformes order. Unexpectedly, a similar minisatellite was found in the first intron of the GH gene in flounder, which belongs to a different order. Transfection of constructs containing a reporter gene and first intron of different length to four cell lines resulted in an inhibitory effect of the longer intron relative to the short intron. A (CA)n microsatellite (saGHpCA) is found in the GH promoter. A similar repeat at the same location is present in GH promoters of several other fish species. High variability (11 alleles) of the saGHpCA was found in a hatchery population. Full‐sib family genotyping showed a Mendelian inheritance of these alleles. A significant association was found between allele distribution and body mass in large and average size fishes from a hatchery population. The intron minisatellites may serve as markers for hybrid population and parental assignment. Its presence in families and orders of the higher teleosts may help solving classification uncertainties. Their conservation and inhibitory effect suggest a biological role. The saGHpCA is correlated with growth and may be a good candidate for predicting growth performance.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>R. Almuly</name><affiliations><json:string>(National Institute of Oceanography, Israel Oceanographic & Limnological Research, Haifa, Israel;</json:string></affiliations></json:item><json:item><name>Y. 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Cavari</name><affiliations><json:string>(National Institute of Oceanography, Israel Oceanographic & Limnological Research, Haifa, Israel;</json:string></affiliations></json:item><json:item><name>B. Rapoport</name><affiliations><json:string>(National Institute of Oceanography, Israel Oceanographic & Limnological Research, Haifa, Israel;</json:string></affiliations></json:item><json:item><name>M. Soller</name><affiliations><json:string>Department of Genetics, The Alexander Silberman Life Sciences Institute, The Hebrew University of Jerusalem, Jerusalem, Israel).</json:string></affiliations></json:item><json:item><name>B. Funkenstein</name><affiliations><json:string>(National Institute of Oceanography, Israel Oceanographic & Limnological Research, Haifa, Israel;</json:string></affiliations></json:item></author><articleId><json:string>JFB559AB</json:string></articleId><language><json:string>eng</json:string></language><originalGenre><json:string>abstract</json:string></originalGenre><abstract>The GH gene of Sparus aurata(saGH) contains variable number of tandem repeats (VNTR). The hyper‐variable minisatellites in the first and third introns segregate in a Mendelian manner and exhibit numerous alleles. Analysis by PCR and sequencing of the two introns in several wild Sparidae species revealed comparable minisatellites with some variations. ‘Zoo blot’ with the first intron unit as a probe showed this sequence to be characteristic of several families from the Perciformes order. Unexpectedly, a similar minisatellite was found in the first intron of the GH gene in flounder, which belongs to a different order. Transfection of constructs containing a reporter gene and first intron of different length to four cell lines resulted in an inhibitory effect of the longer intron relative to the short intron. A (CA)n microsatellite (saGHpCA) is found in the GH promoter. A similar repeat at the same location is present in GH promoters of several other fish species. High variability (11 alleles) of the saGHpCA was found in a hatchery population. Full‐sib family genotyping showed a Mendelian inheritance of these alleles. A significant association was found between allele distribution and body mass in large and average size fishes from a hatchery population. The intron minisatellites may serve as markers for hybrid population and parental assignment. Its presence in families and orders of the higher teleosts may help solving classification uncertainties. 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The hyper‐variable minisatellites in the first and third introns segregate in a Mendelian manner and exhibit numerous alleles. Analysis by PCR and sequencing of the two introns in several wild Sparidae species revealed comparable minisatellites with some variations. ‘Zoo blot’ with the first intron unit as a probe showed this sequence to be characteristic of several families from the Perciformes order. Unexpectedly, a similar minisatellite was found in the first intron of the GH gene in flounder, which belongs to a different order. Transfection of constructs containing a reporter gene and first intron of different length to four cell lines resulted in an inhibitory effect of the longer intron relative to the short intron. A (CA)n microsatellite (saGHpCA) is found in the GH promoter. A similar repeat at the same location is present in GH promoters of several other fish species. High variability (11 alleles) of the saGHpCA was found in a hatchery population. Full‐sib family genotyping showed a Mendelian inheritance of these alleles. A significant association was found between allele distribution and body mass in large and average size fishes from a hatchery population. The intron minisatellites may serve as markers for hybrid population and parental assignment. Its presence in families and orders of the higher teleosts may help solving classification uncertainties. Their conservation and inhibitory effect suggest a biological role. 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affiliationRef="#a4"><personName><givenNames>M.</givenNames><familyName>Soller</familyName></personName></creator><creator creatorRole="author" xml:id="cr10" affiliationRef="#a1"><personName><givenNames>B.</givenNames><familyName>Funkenstein</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="a1" countryCode="IL"><unparsedAffiliation>(<i>National Institute of Oceanography, Israel Oceanographic & Limnological Research, Haifa, Israel; </i></unparsedAffiliation></affiliation><affiliation xml:id="a2" countryCode="IL"><unparsedAffiliation><i>Department of Food Engineering and Biotechnology, The Technion, Haifa, Israel; </i></unparsedAffiliation></affiliation><affiliation xml:id="a3" countryCode="IL"><unparsedAffiliation><i>National Center of Mariculture, Israel Oceanographic & Limnological Research, Eilat, Israel; </i></unparsedAffiliation></affiliation><affiliation xml:id="a4" countryCode="IL"><unparsedAffiliation><i>Department of Genetics, The Alexander Silberman Life Sciences Institute, The Hebrew University of Jerusalem, Jerusalem, Israel</i>).</unparsedAffiliation></affiliation></affiliationGroup><abstractGroup><abstract type="main" xml:lang="en"><p>The GH gene of <i>Sparus aurata</i>(saGH) contains variable number of tandem repeats (VNTR). The hyper‐variable minisatellites in the first and third introns segregate in a Mendelian manner and exhibit numerous alleles. Analysis by PCR and sequencing of the two introns in several wild Sparidae species revealed comparable minisatellites with some variations. ‘Zoo blot’ with the first intron unit as a probe showed this sequence to be characteristic of several families from the Perciformes order. Unexpectedly, a similar minisatellite was found in the first intron of the GH gene in flounder, which belongs to a different order. Transfection of constructs containing a reporter gene and first intron of different length to four cell lines resulted in an inhibitory effect of the longer intron relative to the short intron. A (CA)n microsatellite (saGHpCA) is found in the GH promoter. A similar repeat at the same location is present in GH promoters of several other fish species. High variability (11 alleles) of the saGHpCA was found in a hatchery population. Full‐sib family genotyping showed a Mendelian inheritance of these alleles. A significant association was found between allele distribution and body mass in large and average size fishes from a hatchery population. The intron minisatellites may serve as markers for hybrid population and parental assignment. Its presence in families and orders of the higher teleosts may help solving classification uncertainties. Their conservation and inhibitory effect suggest a biological role. The saGHpCA is correlated with growth and may be a good candidate for predicting growth performance.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Variable number of tandem repeats in the growth hormone gene of Sparus aurata: association with growth and effect on gene transcription</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>poster abstracts</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Variable number of tandem repeats in the growth hormone gene of Sparus aurata: association with growth and effect on gene transcription</title></titleInfo><name type="personal"><namePart type="given">R.</namePart><namePart type="family">Almuly</namePart><affiliation>(National Institute of Oceanography, Israel Oceanographic & Limnological Research, Haifa, Israel;</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Y.</namePart><namePart type="family">Poleg‐Danin</namePart><affiliation>Department of Food Engineering and Biotechnology, The Technion, Haifa, Israel;</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Y.</namePart><namePart type="family">Kashi</namePart><affiliation>Department of Food Engineering and Biotechnology, The Technion, Haifa, Israel;</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S.</namePart><namePart type="family">Gorshkov</namePart><affiliation>National Center of Mariculture, Israel Oceanographic & Limnological Research, Eilat, Israel;</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">G.</namePart><namePart type="family">Gorshkova</namePart><affiliation>National Center of Mariculture, Israel Oceanographic & Limnological Research, Eilat, Israel;</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">A.</namePart><namePart type="family">Dyman</namePart><affiliation>(National Institute of Oceanography, Israel Oceanographic & Limnological Research, Haifa, Israel;</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">B.</namePart><namePart type="family">Cavari</namePart><affiliation>(National Institute of Oceanography, Israel Oceanographic & Limnological Research, Haifa, Israel;</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">B.</namePart><namePart type="family">Rapoport</namePart><affiliation>(National Institute of Oceanography, Israel Oceanographic & Limnological Research, Haifa, Israel;</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Soller</namePart><affiliation>Department of Genetics, The Alexander Silberman Life Sciences Institute, The Hebrew University of Jerusalem, Jerusalem, Israel).</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">B.</namePart><namePart type="family">Funkenstein</namePart><affiliation>(National Institute of Oceanography, Israel Oceanographic & Limnological Research, Haifa, Israel;</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="abstract" displayLabel="abstract"></genre><originInfo><publisher>Blackwell Science Ltd</publisher><place><placeTerm type="text">Oxford, UK; Malden, USA</placeTerm></place><dateIssued encoding="w3cdtf">2004-12</dateIssued><copyrightDate encoding="w3cdtf">2004</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">The GH gene of Sparus aurata(saGH) contains variable number of tandem repeats (VNTR). The hyper‐variable minisatellites in the first and third introns segregate in a Mendelian manner and exhibit numerous alleles. Analysis by PCR and sequencing of the two introns in several wild Sparidae species revealed comparable minisatellites with some variations. ‘Zoo blot’ with the first intron unit as a probe showed this sequence to be characteristic of several families from the Perciformes order. Unexpectedly, a similar minisatellite was found in the first intron of the GH gene in flounder, which belongs to a different order. Transfection of constructs containing a reporter gene and first intron of different length to four cell lines resulted in an inhibitory effect of the longer intron relative to the short intron. A (CA)n microsatellite (saGHpCA) is found in the GH promoter. A similar repeat at the same location is present in GH promoters of several other fish species. High variability (11 alleles) of the saGHpCA was found in a hatchery population. Full‐sib family genotyping showed a Mendelian inheritance of these alleles. A significant association was found between allele distribution and body mass in large and average size fishes from a hatchery population. The intron minisatellites may serve as markers for hybrid population and parental assignment. Its presence in families and orders of the higher teleosts may help solving classification uncertainties. Their conservation and inhibitory effect suggest a biological role. The saGHpCA is correlated with growth and may be a good candidate for predicting growth performance.</abstract><relatedItem type="host"><titleInfo><title>Journal of Fish Biology</title></titleInfo><genre type="journal">journal</genre><identifier type="ISSN">0022-1112</identifier><identifier type="eISSN">1095-8649</identifier><identifier type="DOI">10.1111/(ISSN)1095-8649</identifier><identifier type="PublisherID">JFB</identifier><part><date>2004</date><detail type="volume"><caption>vol.</caption><number>65</number></detail><detail type="supplement"><caption>Suppl. no.</caption><number>s1</number></detail><extent unit="pages"><start>326</start><end>326</end><total>1</total></extent></part></relatedItem><identifier type="istex">F17ECAC340969B2F7E8F5D869A6771078055BC8F</identifier><identifier type="DOI">10.1111/j.0022-1112.2004.00559ab.x</identifier><identifier type="ArticleID">JFB559AB</identifier><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>Blackwell Science Ltd</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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