Impact of dietary protein hydrolysates on skeleton quality and proteome in Diplodus sargus larvae
Identifieur interne : 001435 ( Istex/Corpus ); précédent : 001434; suivant : 001436Impact of dietary protein hydrolysates on skeleton quality and proteome in Diplodus sargus larvae
Auteurs : M. De Vareilles ; N. Richard ; P. J. Gavaia ; T. S. Silva ; O. Cordeiro ; I. Guerreiro ; M. Yúfera ; I. Batista ; C. Pires ; P. Pousão-Ferreira ; P. M. Rodrigues ; I. R Nnestad ; K. E. Fladmark ; L. E. C. ConceiçãoSource :
- Journal of Applied Ichthyology [ 0175-8659 ] ; 2012-06.
Abstract
In order to investigate the effects of dietary protein hydrolysates (PH) on larval growth performance, skeleton quality and proteome expression, triplicate groups of white seabream (Diplodus sargus) larvae were co‐fed from first‐feeding with live feed and three microencapsulated diets differing in the molecular weight of their PH fraction (Control – inclusion of CPSP‐90; H – inclusion of a high amount in 0.5–30 kDa hydrolysates; L – inclusion of a high amount in <0.5 kDa hydrolysates). At 15 days after hatching (DAH), proteome expression changes were assessed in entire larvae by two‐dimensional gel electrophoresis and the quality of larval skeleton was analysed at 28 DAH through double staining of cartilage and bone. Dietary PH fractions tested affected growth, the larvae fed diet L being significantly larger than those fed diet H, but it did not affect the incidence of deformed larvae, nor the number of deformities per fish. Two‐dimensional analysis of larvae proteome allowed the detection and the comparative quantification of a total of 709 protein spots having a pI between 4 and 7, around half of which had an expression significantly affected by dietary treatment, the main difference being between proteome of Control larvae with those of both groups L and H. From these spots, 52 proteins involved in diverse processes such as cytoskeletal dynamics, energetic, lipoprotein, amino acid (AA), and nucleotide metabolisms, protein chaperoning and degradation, and signal transduction, were identified. This study revealed that the molecular weight of the dietary protein hydrolysate fraction had a minor impact on skeletal deformities in white seabream larvae, but affected growth performance and had a strong impact on larvae whole body proteome.
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DOI: 10.1111/j.1439-0426.2012.01986.x
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De Vareilles</name><affiliation><mods:affiliation>CIMAR/CCMAR, Universidade do Algarve, Campus de Gambelas, Faro, Portugal</mods:affiliation></affiliation><affiliation><mods:affiliation>Department of Biology, University of Bergen, Bergen, Norway</mods:affiliation></affiliation></author><author><name sortKey="Richard, N" sort="Richard, N" uniqKey="Richard N" first="N." last="Richard">N. Richard</name><affiliation><mods:affiliation>CIMAR/CCMAR, Universidade do Algarve, Campus de Gambelas, Faro, Portugal</mods:affiliation></affiliation></author><author><name sortKey="Gavaia, P J" sort="Gavaia, P J" uniqKey="Gavaia P" first="P. J." last="Gavaia">P. J. Gavaia</name><affiliation><mods:affiliation>CIMAR/CCMAR, Universidade do Algarve, Campus de Gambelas, Faro, Portugal</mods:affiliation></affiliation></author><author><name sortKey="Silva, T S" sort="Silva, T S" uniqKey="Silva T" first="T. S." last="Silva">T. S. 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Pousão-Ferreira</name><affiliation><mods:affiliation>Instituto Nacional dos Recursos Biológicos (INRB/IPIMAR SUL), Olhão, Portugal</mods:affiliation></affiliation></author><author><name sortKey="Rodrigues, P M" sort="Rodrigues, P M" uniqKey="Rodrigues P" first="P. M." last="Rodrigues">P. M. Rodrigues</name><affiliation><mods:affiliation>CIMAR/CCMAR, Universidade do Algarve, Campus de Gambelas, Faro, Portugal</mods:affiliation></affiliation></author><author><name sortKey="R Nnestad, I" sort="R Nnestad, I" uniqKey="R Nnestad I" first="I." last="R Nnestad">I. R Nnestad</name><affiliation><mods:affiliation>Department of Biology, University of Bergen, Bergen, Norway</mods:affiliation></affiliation></author><author><name sortKey="Fladmark, K E" sort="Fladmark, K E" uniqKey="Fladmark K" first="K. E." last="Fladmark">K. E. Fladmark</name><affiliation><mods:affiliation>Department of Molecular Biology, University of Bergen, Bergen, Norway</mods:affiliation></affiliation></author><author><name sortKey="Conceicao, L E C" sort="Conceicao, L E C" uniqKey="Conceicao L" first="L. E. C." last="Conceição">L. E. C. Conceição</name><affiliation><mods:affiliation>CIMAR/CCMAR, Universidade do Algarve, Campus de Gambelas, Faro, Portugal</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Applied Ichthyology</title><idno type="ISSN">0175-8659</idno><idno type="eISSN">1439-0426</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2012-06">2012-06</date><biblScope unit="volume">28</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="477">477</biblScope><biblScope unit="page" to="487">487</biblScope></imprint><idno type="ISSN">0175-8659</idno></series><idno type="istex">97BDFDA4A65E7382A81D31D149DFEB0D50395A37</idno><idno type="DOI">10.1111/j.1439-0426.2012.01986.x</idno><idno type="ArticleID">JAI1986</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0175-8659</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In order to investigate the effects of dietary protein hydrolysates (PH) on larval growth performance, skeleton quality and proteome expression, triplicate groups of white seabream (Diplodus sargus) larvae were co‐fed from first‐feeding with live feed and three microencapsulated diets differing in the molecular weight of their PH fraction (Control – inclusion of CPSP‐90; H – inclusion of a high amount in 0.5–30 kDa hydrolysates; L – inclusion of a high amount in <0.5 kDa hydrolysates). At 15 days after hatching (DAH), proteome expression changes were assessed in entire larvae by two‐dimensional gel electrophoresis and the quality of larval skeleton was analysed at 28 DAH through double staining of cartilage and bone. Dietary PH fractions tested affected growth, the larvae fed diet L being significantly larger than those fed diet H, but it did not affect the incidence of deformed larvae, nor the number of deformities per fish. Two‐dimensional analysis of larvae proteome allowed the detection and the comparative quantification of a total of 709 protein spots having a pI between 4 and 7, around half of which had an expression significantly affected by dietary treatment, the main difference being between proteome of Control larvae with those of both groups L and H. From these spots, 52 proteins involved in diverse processes such as cytoskeletal dynamics, energetic, lipoprotein, amino acid (AA), and nucleotide metabolisms, protein chaperoning and degradation, and signal transduction, were identified. This study revealed that the molecular weight of the dietary protein hydrolysate fraction had a minor impact on skeletal deformities in white seabream larvae, but affected growth performance and had a strong impact on larvae whole body proteome.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>M. de Vareilles</name><affiliations><json:string>CIMAR/CCMAR, Universidade do Algarve, Campus de Gambelas, Faro, Portugal</json:string><json:string>Department of Biology, University of Bergen, Bergen, Norway</json:string></affiliations></json:item><json:item><name>N. Richard</name><affiliations><json:string>CIMAR/CCMAR, Universidade do Algarve, Campus de Gambelas, Faro, Portugal</json:string></affiliations></json:item><json:item><name>P. J. Gavaia</name><affiliations><json:string>CIMAR/CCMAR, Universidade do Algarve, Campus de Gambelas, Faro, Portugal</json:string></affiliations></json:item><json:item><name>T. S. Silva</name><affiliations><json:string>CIMAR/CCMAR, Universidade do Algarve, Campus de Gambelas, Faro, Portugal</json:string></affiliations></json:item><json:item><name>O. Cordeiro</name><affiliations><json:string>CIMAR/CCMAR, Universidade do Algarve, Campus de Gambelas, Faro, Portugal</json:string></affiliations></json:item><json:item><name>I. Guerreiro</name><affiliations><json:string>CIMAR/CCMAR, Universidade do Algarve, Campus de Gambelas, Faro, Portugal</json:string></affiliations></json:item><json:item><name>M. Yúfera</name><affiliations><json:string>Instituto de Ciencias Marinas de Andalucía (ICMAN‐CSIC), Cádiz, Spain</json:string></affiliations></json:item><json:item><name>I. Batista</name><affiliations><json:string>Instituto Nacional dos Recursos Biológicos (INRB/IPIMAR), Lisboa, Portugal</json:string></affiliations></json:item><json:item><name>C. Pires</name><affiliations><json:string>Instituto Nacional dos Recursos Biológicos (INRB/IPIMAR), Lisboa, Portugal</json:string></affiliations></json:item><json:item><name>P. Pousão‐Ferreira</name><affiliations><json:string>Instituto Nacional dos Recursos Biológicos (INRB/IPIMAR SUL), Olhão, Portugal</json:string></affiliations></json:item><json:item><name>P. M. Rodrigues</name><affiliations><json:string>CIMAR/CCMAR, Universidade do Algarve, Campus de Gambelas, Faro, Portugal</json:string></affiliations></json:item><json:item><name>I. Rønnestad</name><affiliations><json:string>Department of Biology, University of Bergen, Bergen, Norway</json:string></affiliations></json:item><json:item><name>K. E. Fladmark</name><affiliations><json:string>Department of Molecular Biology, University of Bergen, Bergen, Norway</json:string></affiliations></json:item><json:item><name>L. E. C. Conceição</name><affiliations><json:string>CIMAR/CCMAR, Universidade do Algarve, Campus de Gambelas, Faro, Portugal</json:string></affiliations></json:item></author><articleId><json:string>JAI1986</json:string></articleId><language><json:string>eng</json:string></language><originalGenre><json:string>article</json:string></originalGenre><abstract>In order to investigate the effects of dietary protein hydrolysates (PH) on larval growth performance, skeleton quality and proteome expression, triplicate groups of white seabream (Diplodus sargus) larvae were co‐fed from first‐feeding with live feed and three microencapsulated diets differing in the molecular weight of their PH fraction (Control – inclusion of CPSP‐90; H – inclusion of a high amount in 0.5–30 kDa hydrolysates; L – inclusion of a high amount in >0.5 kDa hydrolysates). At 15 days after hatching (DAH), proteome expression changes were assessed in entire larvae by two‐dimensional gel electrophoresis and the quality of larval skeleton was analysed at 28 DAH through double staining of cartilage and bone. Dietary PH fractions tested affected growth, the larvae fed diet L being significantly larger than those fed diet H, but it did not affect the incidence of deformed larvae, nor the number of deformities per fish. Two‐dimensional analysis of larvae proteome allowed the detection and the comparative quantification of a total of 709 protein spots having a pI between 4 and 7, around half of which had an expression significantly affected by dietary treatment, the main difference being between proteome of Control larvae with those of both groups L and H. From these spots, 52 proteins involved in diverse processes such as cytoskeletal dynamics, energetic, lipoprotein, amino acid (AA), and nucleotide metabolisms, protein chaperoning and degradation, and signal transduction, were identified. This study revealed that the molecular weight of the dietary protein hydrolysate fraction had a minor impact on skeletal deformities in white seabream larvae, but affected growth performance and had a strong impact on larvae whole body proteome.</abstract><qualityIndicators><score>8</score><pdfVersion>1.3</pdfVersion><pdfPageSize>595.276 x 841.89 pts (A4)</pdfPageSize><refBibsNative>true</refBibsNative><abstractCharCount>1766</abstractCharCount><pdfWordCount>7783</pdfWordCount><pdfCharCount>51956</pdfCharCount><pdfPageCount>11</pdfPageCount><abstractWordCount>266</abstractWordCount></qualityIndicators><title>Impact of dietary protein hydrolysates on skeleton quality and proteome in Diplodus sargus larvae</title><genre><json:string>article</json:string></genre><host><volume>28</volume><publisherId><json:string>JAI</json:string></publisherId><pages><total>11</total><last>487</last><first>477</first></pages><issn><json:string>0175-8659</json:string></issn><issue>3</issue><genre><json:string>journal</json:string></genre><language><json:string>unknown</json:string></language><eissn><json:string>1439-0426</json:string></eissn><title>Journal of Applied Ichthyology</title><doi><json:string>10.1111/(ISSN)1439-0426</json:string></doi></host><categories><wos><json:string>science</json:string><json:string>marine & freshwater biology</json:string><json:string>fisheries</json:string></wos><scienceMetrix><json:string>applied sciences</json:string><json:string>agriculture, fisheries & forestry</json:string><json:string>fisheries</json:string></scienceMetrix></categories><publicationDate>2012</publicationDate><copyrightDate>2012</copyrightDate><doi><json:string>10.1111/j.1439-0426.2012.01986.x</json:string></doi><id>97BDFDA4A65E7382A81D31D149DFEB0D50395A37</id><score>0.014626235</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/document/97BDFDA4A65E7382A81D31D149DFEB0D50395A37/fulltext/pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/document/97BDFDA4A65E7382A81D31D149DFEB0D50395A37/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/97BDFDA4A65E7382A81D31D149DFEB0D50395A37/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Impact of dietary protein hydrolysates on skeleton quality and proteome in Diplodus sargus larvae</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><availability><p>© 2012 Blackwell Verlag, Berlin</p></availability><date>2012</date></publicationStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Impact of dietary protein hydrolysates on skeleton quality and proteome in Diplodus sargus larvae</title><author xml:id="author-1"><persName><forename type="first">M.</forename><surname>de Vareilles</surname></persName><note type="biography">These authors have contributed equally to this work.</note><affiliation>These authors have contributed equally to this work.</affiliation><affiliation>CIMAR/CCMAR, Universidade do Algarve, Campus de Gambelas, Faro, Portugal</affiliation><affiliation>Department of Biology, University of Bergen, Bergen, Norway</affiliation></author><author xml:id="author-2"><persName><forename type="first">N.</forename><surname>Richard</surname></persName><note type="biography">These authors have contributed equally to this work.</note><affiliation>These authors have contributed equally to this work.</affiliation><affiliation>CIMAR/CCMAR, Universidade do Algarve, Campus de Gambelas, Faro, Portugal</affiliation></author><author xml:id="author-3"><persName><forename type="first">P. J.</forename><surname>Gavaia</surname></persName><affiliation>CIMAR/CCMAR, Universidade do Algarve, Campus de Gambelas, Faro, Portugal</affiliation></author><author xml:id="author-4"><persName><forename type="first">T. S.</forename><surname>Silva</surname></persName><affiliation>CIMAR/CCMAR, Universidade do Algarve, Campus de Gambelas, Faro, Portugal</affiliation></author><author xml:id="author-5"><persName><forename type="first">O.</forename><surname>Cordeiro</surname></persName><affiliation>CIMAR/CCMAR, Universidade do Algarve, Campus de Gambelas, Faro, Portugal</affiliation></author><author xml:id="author-6"><persName><forename type="first">I.</forename><surname>Guerreiro</surname></persName><affiliation>CIMAR/CCMAR, Universidade do Algarve, Campus de Gambelas, Faro, Portugal</affiliation></author><author xml:id="author-7"><persName><forename type="first">M.</forename><surname>Yúfera</surname></persName><affiliation>Instituto de Ciencias Marinas de Andalucía (ICMAN‐CSIC), Cádiz, Spain</affiliation></author><author xml:id="author-8"><persName><forename type="first">I.</forename><surname>Batista</surname></persName><affiliation>Instituto Nacional dos Recursos Biológicos (INRB/IPIMAR), Lisboa, Portugal</affiliation></author><author xml:id="author-9"><persName><forename type="first">C.</forename><surname>Pires</surname></persName><affiliation>Instituto Nacional dos Recursos Biológicos (INRB/IPIMAR), Lisboa, Portugal</affiliation></author><author xml:id="author-10"><persName><forename type="first">P.</forename><surname>Pousão‐Ferreira</surname></persName><affiliation>Instituto Nacional dos Recursos Biológicos (INRB/IPIMAR SUL), Olhão, Portugal</affiliation></author><author xml:id="author-11"><persName><forename type="first">P. M.</forename><surname>Rodrigues</surname></persName><affiliation>CIMAR/CCMAR, Universidade do Algarve, Campus de Gambelas, Faro, Portugal</affiliation></author><author xml:id="author-12"><persName><forename type="first">I.</forename><surname>Rønnestad</surname></persName><affiliation>Department of Biology, University of Bergen, Bergen, Norway</affiliation></author><author xml:id="author-13"><persName><forename type="first">K. E.</forename><surname>Fladmark</surname></persName><affiliation>Department of Molecular Biology, University of Bergen, Bergen, Norway</affiliation></author><author xml:id="author-14"><persName><forename type="first">L. E. C.</forename><surname>Conceição</surname></persName><affiliation>CIMAR/CCMAR, Universidade do Algarve, Campus de Gambelas, Faro, Portugal</affiliation></author></analytic><monogr><title level="j">Journal of Applied Ichthyology</title><idno type="pISSN">0175-8659</idno><idno type="eISSN">1439-0426</idno><idno type="DOI">10.1111/(ISSN)1439-0426</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2012-06"></date><biblScope unit="volume">28</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="477">477</biblScope><biblScope unit="page" to="487">487</biblScope></imprint></monogr><idno type="istex">97BDFDA4A65E7382A81D31D149DFEB0D50395A37</idno><idno type="DOI">10.1111/j.1439-0426.2012.01986.x</idno><idno type="ArticleID">JAI1986</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2012</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>In order to investigate the effects of dietary protein hydrolysates (PH) on larval growth performance, skeleton quality and proteome expression, triplicate groups of white seabream (Diplodus sargus) larvae were co‐fed from first‐feeding with live feed and three microencapsulated diets differing in the molecular weight of their PH fraction (Control – inclusion of CPSP‐90; H – inclusion of a high amount in 0.5–30 kDa hydrolysates; L – inclusion of a high amount in <0.5 kDa hydrolysates). At 15 days after hatching (DAH), proteome expression changes were assessed in entire larvae by two‐dimensional gel electrophoresis and the quality of larval skeleton was analysed at 28 DAH through double staining of cartilage and bone. Dietary PH fractions tested affected growth, the larvae fed diet L being significantly larger than those fed diet H, but it did not affect the incidence of deformed larvae, nor the number of deformities per fish. Two‐dimensional analysis of larvae proteome allowed the detection and the comparative quantification of a total of 709 protein spots having a pI between 4 and 7, around half of which had an expression significantly affected by dietary treatment, the main difference being between proteome of Control larvae with those of both groups L and H. From these spots, 52 proteins involved in diverse processes such as cytoskeletal dynamics, energetic, lipoprotein, amino acid (AA), and nucleotide metabolisms, protein chaperoning and degradation, and signal transduction, were identified. This study revealed that the molecular weight of the dietary protein hydrolysate fraction had a minor impact on skeletal deformities in white seabream larvae, but affected growth performance and had a strong impact on larvae whole body proteome.</p></abstract></profileDesc><revisionDesc><change when="2012-06">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/97BDFDA4A65E7382A81D31D149DFEB0D50395A37/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>Blackwell Publishing Ltd</publisherName><publisherLoc>Oxford, UK</publisherLoc></publisherInfo><doi origin="wiley" registered="yes">10.1111/(ISSN)1439-0426</doi><issn type="print">0175-8659</issn><issn type="electronic">1439-0426</issn><idGroup><id type="product" value="JAI"></id><id type="publisherDivision" value="ST"></id></idGroup><titleGroup><title type="main" sort="JOURNAL OF APPLIED ICHTHYOLOGY">Journal of Applied Ichthyology</title></titleGroup></publicationMeta><publicationMeta level="part" position="06103"><doi origin="wiley">10.1111/jai.2012.28.issue-3</doi><titleGroup><title type="specialIssueTitle">Interdisciplinary Approaches in Fish Skeletal Biology Proceedings of the Second Conference Tavira, Algarve, Portugal April 26‐28, 2011</title></titleGroup><numberingGroup><numbering type="journalVolume" number="28">28</numbering><numbering type="journalIssue">3</numbering></numberingGroup><coverDate startDate="2012-06">June 2012</coverDate></publicationMeta><publicationMeta level="unit" type="article" position="33" status="forIssue"><doi origin="wiley">10.1111/j.1439-0426.2012.01986.x</doi><idGroup><id type="unit" value="JAI1986"></id></idGroup><countGroup><count type="pageTotal" number="11"></count></countGroup><titleGroup><title type="tocHeading1">Malformations</title></titleGroup><copyright>© 2012 Blackwell Verlag, Berlin</copyright><eventGroup><event type="xmlConverted" agent="Converter:BPG_TO_WML3G version:3.1.5 mode:FullText" date="2012-05-25"></event><event type="publishedOnlineFinalForm" date="2012-05-21"></event><event type="firstOnline" date="2012-05-21"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:3.8.8" date="2014-01-28"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.3.4 mode:FullText" date="2015-02-25"></event></eventGroup><numberingGroup><numbering type="pageFirst" number="477">477</numbering><numbering type="pageLast" number="487">487</numbering></numberingGroup><correspondenceTo><b>Author’s address:</b> Mahaut de Vareilles, CIMAR/CCMAR, Universidade do Algarve, Campus de Gambelas, 8000‐139 Faro, Portugal.
E‐mail: <email>mdvareilles@ualg.pt</email></correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:JAI.JAI1986.pdf"></link></linkGroup></publicationMeta><contentMeta><unparsedEditorialHistory>Received: September 23, 2011 Accepted: March 08, 2012</unparsedEditorialHistory><countGroup><count type="figureTotal" number="4"></count><count type="tableTotal" number="4"></count></countGroup><titleGroup><title type="main">Impact of dietary protein hydrolysates on skeleton quality and proteome in <i>Diplodus sargus</i> larvae</title><title type="shortAuthors">M. de Vareilles et al.</title><title type="short">Dietary protein: skeleton and proteome in larvae</title></titleGroup><creators><creator creatorRole="author" xml:id="cr1" affiliationRef="#a1 #a2" noteRef="#fn1"><personName><givenNames>M.</givenNames><familyName>de Vareilles</familyName></personName></creator><creator creatorRole="author" xml:id="cr2" affiliationRef="#a1" noteRef="#fn1"><personName><givenNames>N.</givenNames><familyName>Richard</familyName></personName></creator><creator creatorRole="author" xml:id="cr3" affiliationRef="#a1"><personName><givenNames>P. J.</givenNames><familyName>Gavaia</familyName></personName></creator><creator creatorRole="author" xml:id="cr4" affiliationRef="#a1"><personName><givenNames>T. S.</givenNames><familyName>Silva</familyName></personName></creator><creator creatorRole="author" xml:id="cr5" affiliationRef="#a1"><personName><givenNames>O.</givenNames><familyName>Cordeiro</familyName></personName></creator><creator creatorRole="author" xml:id="cr6" affiliationRef="#a1"><personName><givenNames>I.</givenNames><familyName>Guerreiro</familyName></personName></creator><creator creatorRole="author" xml:id="cr7" affiliationRef="#a3"><personName><givenNames>M.</givenNames><familyName>Yúfera</familyName></personName></creator><creator creatorRole="author" xml:id="cr8" affiliationRef="#a4"><personName><givenNames>I.</givenNames><familyName>Batista</familyName></personName></creator><creator creatorRole="author" xml:id="cr9" affiliationRef="#a4"><personName><givenNames>C.</givenNames><familyName>Pires</familyName></personName></creator><creator creatorRole="author" xml:id="cr10" affiliationRef="#a5"><personName><givenNames>P.</givenNames><familyName>Pousão‐Ferreira</familyName></personName></creator><creator creatorRole="author" xml:id="cr11" affiliationRef="#a1"><personName><givenNames>P. M.</givenNames><familyName>Rodrigues</familyName></personName></creator><creator creatorRole="author" xml:id="cr12" affiliationRef="#a2"><personName><givenNames>I.</givenNames><familyName>Rønnestad</familyName></personName></creator><creator creatorRole="author" xml:id="cr13" affiliationRef="#a6"><personName><givenNames>K. E.</givenNames><familyName>Fladmark</familyName></personName></creator><creator creatorRole="author" xml:id="cr14" affiliationRef="#a1"><personName><givenNames>L. E. C.</givenNames><familyName>Conceição</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="a1" countryCode="PT"><unparsedAffiliation>CIMAR/CCMAR, Universidade do Algarve, Campus de Gambelas, Faro, Portugal</unparsedAffiliation></affiliation><affiliation xml:id="a2" countryCode="NO"><unparsedAffiliation>Department of Biology, University of Bergen, Bergen, Norway</unparsedAffiliation></affiliation><affiliation xml:id="a3" countryCode="ES"><unparsedAffiliation>Instituto de Ciencias Marinas de Andalucía (ICMAN‐CSIC), Cádiz, Spain</unparsedAffiliation></affiliation><affiliation xml:id="a4" countryCode="PT"><unparsedAffiliation>Instituto Nacional dos Recursos Biológicos (INRB/IPIMAR), Lisboa, Portugal</unparsedAffiliation></affiliation><affiliation xml:id="a5" countryCode="PT"><unparsedAffiliation>Instituto Nacional dos Recursos Biológicos (INRB/IPIMAR SUL), Olhão, Portugal</unparsedAffiliation></affiliation><affiliation xml:id="a6" countryCode="NO"><unparsedAffiliation>Department of Molecular Biology, University of Bergen, Bergen, Norway</unparsedAffiliation></affiliation></affiliationGroup><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Summary</title><p>In order to investigate the effects of dietary protein hydrolysates (PH) on larval growth performance, skeleton quality and proteome expression, triplicate groups of white seabream (<i>Diplodus sargus</i>) larvae were co‐fed from first‐feeding with live feed and three microencapsulated diets differing in the molecular weight of their PH fraction (Control – inclusion of CPSP‐90; H – inclusion of a high amount in 0.5–30 kDa hydrolysates; L – inclusion of a high amount in <0.5 kDa hydrolysates). At 15 days after hatching (DAH), proteome expression changes were assessed in entire larvae by two‐dimensional gel electrophoresis and the quality of larval skeleton was analysed at 28 DAH through double staining of cartilage and bone. Dietary PH fractions tested affected growth, the larvae fed diet L being significantly larger than those fed diet H, but it did not affect the incidence of deformed larvae, nor the number of deformities per fish. Two‐dimensional analysis of larvae proteome allowed the detection and the comparative quantification of a total of 709 protein spots having a pI between 4 and 7, around half of which had an expression significantly affected by dietary treatment, the main difference being between proteome of Control larvae with those of both groups L and H. From these spots, 52 proteins involved in diverse processes such as cytoskeletal dynamics, energetic, lipoprotein, amino acid (AA), and nucleotide metabolisms, protein chaperoning and degradation, and signal transduction, were identified. This study revealed that the molecular weight of the dietary protein hydrolysate fraction had a minor impact on skeletal deformities in white seabream larvae, but affected growth performance and had a strong impact on larvae whole body proteome.</p></abstract></abstractGroup></contentMeta><noteGroup><note xml:id="fn1"><p> These authors have contributed equally to this work.</p></note></noteGroup></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Impact of dietary protein hydrolysates on skeleton quality and proteome in Diplodus sargus larvae</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>Dietary protein: skeleton and proteome in larvae</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Impact of dietary protein hydrolysates on skeleton quality and proteome in Diplodus sargus larvae</title></titleInfo><name type="personal"><namePart type="given">M.</namePart><namePart type="family">de Vareilles</namePart><affiliation>CIMAR/CCMAR, Universidade do Algarve, Campus de Gambelas, Faro, Portugal</affiliation><affiliation>Department of Biology, University of Bergen, Bergen, Norway</affiliation><description>These authors have contributed equally to this work.</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">N.</namePart><namePart type="family">Richard</namePart><affiliation>CIMAR/CCMAR, Universidade do Algarve, Campus de Gambelas, Faro, Portugal</affiliation><description>These authors have contributed equally to this work.</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">P. J.</namePart><namePart type="family">Gavaia</namePart><affiliation>CIMAR/CCMAR, Universidade do Algarve, Campus de Gambelas, Faro, Portugal</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">T. S.</namePart><namePart type="family">Silva</namePart><affiliation>CIMAR/CCMAR, Universidade do Algarve, Campus de Gambelas, Faro, Portugal</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">O.</namePart><namePart type="family">Cordeiro</namePart><affiliation>CIMAR/CCMAR, Universidade do Algarve, Campus de Gambelas, Faro, Portugal</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">I.</namePart><namePart type="family">Guerreiro</namePart><affiliation>CIMAR/CCMAR, Universidade do Algarve, Campus de Gambelas, Faro, Portugal</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Yúfera</namePart><affiliation>Instituto de Ciencias Marinas de Andalucía (ICMAN‐CSIC), Cádiz, Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">I.</namePart><namePart type="family">Batista</namePart><affiliation>Instituto Nacional dos Recursos Biológicos (INRB/IPIMAR), Lisboa, Portugal</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">C.</namePart><namePart type="family">Pires</namePart><affiliation>Instituto Nacional dos Recursos Biológicos (INRB/IPIMAR), Lisboa, Portugal</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">P.</namePart><namePart type="family">Pousão‐Ferreira</namePart><affiliation>Instituto Nacional dos Recursos Biológicos (INRB/IPIMAR SUL), Olhão, Portugal</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">P. M.</namePart><namePart type="family">Rodrigues</namePart><affiliation>CIMAR/CCMAR, Universidade do Algarve, Campus de Gambelas, Faro, Portugal</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">I.</namePart><namePart type="family">Rønnestad</namePart><affiliation>Department of Biology, University of Bergen, Bergen, Norway</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">K. E.</namePart><namePart type="family">Fladmark</namePart><affiliation>Department of Molecular Biology, University of Bergen, Bergen, Norway</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">L. E. C.</namePart><namePart type="family">Conceição</namePart><affiliation>CIMAR/CCMAR, Universidade do Algarve, Campus de Gambelas, Faro, Portugal</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Blackwell Publishing Ltd</publisher><place><placeTerm type="text">Oxford, UK</placeTerm></place><dateIssued encoding="w3cdtf">2012-06</dateIssued><edition>Received: September 23, 2011 Accepted: March 08, 2012</edition><copyrightDate encoding="w3cdtf">2012</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">4</extent><extent unit="tables">4</extent></physicalDescription><abstract lang="en">In order to investigate the effects of dietary protein hydrolysates (PH) on larval growth performance, skeleton quality and proteome expression, triplicate groups of white seabream (Diplodus sargus) larvae were co‐fed from first‐feeding with live feed and three microencapsulated diets differing in the molecular weight of their PH fraction (Control – inclusion of CPSP‐90; H – inclusion of a high amount in 0.5–30 kDa hydrolysates; L – inclusion of a high amount in <0.5 kDa hydrolysates). At 15 days after hatching (DAH), proteome expression changes were assessed in entire larvae by two‐dimensional gel electrophoresis and the quality of larval skeleton was analysed at 28 DAH through double staining of cartilage and bone. Dietary PH fractions tested affected growth, the larvae fed diet L being significantly larger than those fed diet H, but it did not affect the incidence of deformed larvae, nor the number of deformities per fish. Two‐dimensional analysis of larvae proteome allowed the detection and the comparative quantification of a total of 709 protein spots having a pI between 4 and 7, around half of which had an expression significantly affected by dietary treatment, the main difference being between proteome of Control larvae with those of both groups L and H. From these spots, 52 proteins involved in diverse processes such as cytoskeletal dynamics, energetic, lipoprotein, amino acid (AA), and nucleotide metabolisms, protein chaperoning and degradation, and signal transduction, were identified. This study revealed that the molecular weight of the dietary protein hydrolysate fraction had a minor impact on skeletal deformities in white seabream larvae, but affected growth performance and had a strong impact on larvae whole body proteome.</abstract><relatedItem type="host"><titleInfo><title>Journal of Applied Ichthyology</title></titleInfo><genre type="journal">journal</genre><identifier type="ISSN">0175-8659</identifier><identifier type="eISSN">1439-0426</identifier><identifier type="DOI">10.1111/(ISSN)1439-0426</identifier><identifier type="PublisherID">JAI</identifier><part><date>2012</date><detail type="title"><title>Interdisciplinary Approaches in Fish Skeletal Biology Proceedings of the Second Conference Tavira, Algarve, Portugal April 26‐28, 2011</title></detail><detail type="volume"><caption>vol.</caption><number>28</number></detail><detail type="issue"><caption>no.</caption><number>3</number></detail><extent unit="pages"><start>477</start><end>487</end><total>11</total></extent></part></relatedItem><identifier type="istex">97BDFDA4A65E7382A81D31D149DFEB0D50395A37</identifier><identifier type="DOI">10.1111/j.1439-0426.2012.01986.x</identifier><identifier type="ArticleID">JAI1986</identifier><accessCondition type="use and reproduction" contentType="copyright">© 2012 Blackwell Verlag, Berlin</accessCondition><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>Blackwell Publishing Ltd</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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