Effects of p‐nonylphenol, methoxychlor, and endosulfan on vitellogenin induction and expression in sheepshead minnow (Cyprinodon variegatus)
Identifieur interne : 001429 ( Istex/Corpus ); précédent : 001428; suivant : 001430Effects of p‐nonylphenol, methoxychlor, and endosulfan on vitellogenin induction and expression in sheepshead minnow (Cyprinodon variegatus)
Auteurs : Michael J. Hemmer ; Becky L. Hemmer ; Chris J. Bowman ; Kevin J. Kroll ; Leroy C. Folmar ; Dragoslav Marcovich ; Marilynn D. Hoglund ; Nancy D. DenslowSource :
- Environmental Toxicology and Chemistry [ 0730-7268 ] ; 2001-02.
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Abstract
Temporal and dose‐responserelationshipsofvitellogenin(VTG) mRNA nductionand subsequent plasma VTG accumulation were established for sheepshead minnows (Cyprinodon variegatus) treated with p‐nonylphenol (an alkylphenol) and the organochlorine pesticides methoxychlor and endosulfan. Thirty‐two adult male fish per treatment were continuously exposed to measured concentrations of 0.64, 5.4, 11.8, 23.3, and 42.7 μg/L p‐nonylphenol; 1.1, 2.5, 5.6, 12.1, and 18.4 μg/L methoxychlor; and in two separate tests, 15.9, 36.3, 68.8, 162, 277, 403, 590, and 788 ng/L endosulfan using an intermittent flow‐through dosing apparatus. Separate triethylene glycol (50 (μl/L) and 17β‐estradiol (65.1 ng/L) treatments served as the negative and positive controls, respectively. Four fish were randomly sampled from each test concentration on days 2, 5, 13, 21, 35, and 42 of exposure, and levels of hepatic VTG mRNA induction and serum VTG accumulation were determined for each individual. Overall, fish exposed to p‐nonylphenol or methoxychlor demonstrated a rapid, dose‐dependent synthesis of VTG mRNA up to day 5 of exposure, followed by a relatively constant dose‐dependent expression through day 42. Both chemicals showed a dose‐dependent increase in plasma VTG over the entire time course of exposure, with significantly elevated VTG levels by the fifth day of exposure to p‐nonylphenol at concentrations of 5.4 μg/L or greater and to methoxychlor at concentrations of 2.5 μg/L or greater. Exposure to 0.64 μg/L p‐nonylphenol resulted in highly variable plasma VTG levels of less than 6 mg/ml. Exposures with endosulfan failed to induce measurable levels of either hepatic VTG mRNA or serum VTG at the chemical concentrations tested. Our results demonstrate that the sheepshead minnow bioassay is a suitable estuarine/marine teleost model for in vivo screening of potentially estrogenic substances.
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DOI: 10.1002/etc.5620200214
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Hemmer</name><affiliation><mods:affiliation>U.S. Environmental Protection Agency, National Health and Environmental Effects Research Laboratory, Gulf Ecology Division, 1 Sabine Island Drive, Gulf Breeze, Florida 32561</mods:affiliation></affiliation><affiliation><mods:affiliation>U.S. Environmental Protection Agency, National Health and Environmental Effects Research Laboratory, Gulf Ecology Division, 1 Sabine Island Drive, Gulf Breeze, Florida 32561</mods:affiliation></affiliation></author><author><name sortKey="Hemmer, Becky L" sort="Hemmer, Becky L" uniqKey="Hemmer B" first="Becky L." last="Hemmer">Becky L. Hemmer</name><affiliation><mods:affiliation>U.S. Environmental Protection Agency, National Health and Environmental Effects Research Laboratory, Gulf Ecology Division, 1 Sabine Island Drive, Gulf Breeze, Florida 32561</mods:affiliation></affiliation></author><author><name sortKey="Bowman, Chris J" sort="Bowman, Chris J" uniqKey="Bowman C" first="Chris J." last="Bowman">Chris J. 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Thirty‐two adult male fish per treatment were continuously exposed to measured concentrations of 0.64, 5.4, 11.8, 23.3, and 42.7 μg/L p‐nonylphenol; 1.1, 2.5, 5.6, 12.1, and 18.4 μg/L methoxychlor; and in two separate tests, 15.9, 36.3, 68.8, 162, 277, 403, 590, and 788 ng/L endosulfan using an intermittent flow‐through dosing apparatus. Separate triethylene glycol (50 (μl/L) and 17β‐estradiol (65.1 ng/L) treatments served as the negative and positive controls, respectively. Four fish were randomly sampled from each test concentration on days 2, 5, 13, 21, 35, and 42 of exposure, and levels of hepatic VTG mRNA induction and serum VTG accumulation were determined for each individual. Overall, fish exposed to p‐nonylphenol or methoxychlor demonstrated a rapid, dose‐dependent synthesis of VTG mRNA up to day 5 of exposure, followed by a relatively constant dose‐dependent expression through day 42. Both chemicals showed a dose‐dependent increase in plasma VTG over the entire time course of exposure, with significantly elevated VTG levels by the fifth day of exposure to p‐nonylphenol at concentrations of 5.4 μg/L or greater and to methoxychlor at concentrations of 2.5 μg/L or greater. Exposure to 0.64 μg/L p‐nonylphenol resulted in highly variable plasma VTG levels of less than 6 mg/ml. Exposures with endosulfan failed to induce measurable levels of either hepatic VTG mRNA or serum VTG at the chemical concentrations tested. Our results demonstrate that the sheepshead minnow bioassay is a suitable estuarine/marine teleost model for in vivo screening of potentially estrogenic substances.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>Michael J. 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Thirty‐two adult male fish per treatment were continuously exposed to measured concentrations of 0.64, 5.4, 11.8, 23.3, and 42.7 μg/L p‐nonylphenol; 1.1, 2.5, 5.6, 12.1, and 18.4 μg/L methoxychlor; and in two separate tests, 15.9, 36.3, 68.8, 162, 277, 403, 590, and 788 ng/L endosulfan using an intermittent flow‐through dosing apparatus. Separate triethylene glycol (50 (μl/L) and 17β‐estradiol (65.1 ng/L) treatments served as the negative and positive controls, respectively. Four fish were randomly sampled from each test concentration on days 2, 5, 13, 21, 35, and 42 of exposure, and levels of hepatic VTG mRNA induction and serum VTG accumulation were determined for each individual. Overall, fish exposed to p‐nonylphenol or methoxychlor demonstrated a rapid, dose‐dependent synthesis of VTG mRNA up to day 5 of exposure, followed by a relatively constant dose‐dependent expression through day 42. Both chemicals showed a dose‐dependent increase in plasma VTG over the entire time course of exposure, with significantly elevated VTG levels by the fifth day of exposure to p‐nonylphenol at concentrations of 5.4 μg/L or greater and to methoxychlor at concentrations of 2.5 μg/L or greater. Exposure to 0.64 μg/L p‐nonylphenol resulted in highly variable plasma VTG levels of less than 6 mg/ml. Exposures with endosulfan failed to induce measurable levels of either hepatic VTG mRNA or serum VTG at the chemical concentrations tested. Our results demonstrate that the sheepshead minnow bioassay is a suitable estuarine/marine teleost model for in vivo screening of potentially estrogenic substances.</abstract><qualityIndicators><score>8</score><pdfVersion>1.3</pdfVersion><pdfPageSize>612 x 792 pts (letter)</pdfPageSize><refBibsNative>true</refBibsNative><abstractCharCount>1888</abstractCharCount><pdfWordCount>6559</pdfWordCount><pdfCharCount>41410</pdfCharCount><pdfPageCount>8</pdfPageCount><abstractWordCount>270</abstractWordCount></qualityIndicators><title>Effects of p‐nonylphenol, methoxychlor, and endosulfan on vitellogenin induction and expression in sheepshead minnow (Cyprinodon variegatus)</title><genre><json:string>article</json:string></genre><host><volume>20</volume><publisherId><json:string>ETC</json:string></publisherId><pages><total>8</total><last>343</last><first>336</first></pages><issn><json:string>0730-7268</json:string></issn><issue>2</issue><subject><json:item><value>Environmental Toxicology</value></json:item></subject><genre><json:string>journal</json:string></genre><language><json:string>unknown</json:string></language><eissn><json:string>1552-8618</json:string></eissn><title>Environmental Toxicology and Chemistry</title><doi><json:string>10.1002/(ISSN)1552-8618</json:string></doi></host><categories><wos><json:string>science</json:string><json:string>toxicology</json:string><json:string>environmental sciences</json:string></wos><scienceMetrix><json:string>natural sciences</json:string><json:string>earth & environmental sciences</json:string><json:string>environmental sciences</json:string></scienceMetrix></categories><publicationDate>2001</publicationDate><copyrightDate>2001</copyrightDate><doi><json:string>10.1002/etc.5620200214</json:string></doi><id>ECC4505F0A843E4FCE9D8C6B3718D3D1C26C5930</id><score>0.028416079</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/document/ECC4505F0A843E4FCE9D8C6B3718D3D1C26C5930/fulltext/pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/document/ECC4505F0A843E4FCE9D8C6B3718D3D1C26C5930/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/ECC4505F0A843E4FCE9D8C6B3718D3D1C26C5930/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Effects of p‐nonylphenol, methoxychlor, and endosulfan on vitellogenin induction and expression in sheepshead minnow (Cyprinodon variegatus)</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>Wiley Periodicals, Inc.</publisher><pubPlace>Hoboken</pubPlace><availability><p>Copyright © 2001 SETAC</p></availability><date>2001</date></publicationStmt><notesStmt><note type="content">*Contribution 1098, Gulf Ecology Division, Florida, USA. The contents of this document do not necessarily reflect the views or policies of the U.S. Environmental Protection Agency, nor does mention of trade names or commercial products constitute endorsement or recommendation for use.</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Effects of p‐nonylphenol, methoxychlor, and endosulfan on vitellogenin induction and expression in sheepshead minnow (Cyprinodon variegatus)</title><author xml:id="author-1"><persName><forename type="first">Michael J.</forename><surname>Hemmer</surname></persName><affiliation>U.S. Environmental Protection Agency, National Health and Environmental Effects Research Laboratory, Gulf Ecology Division, 1 Sabine Island Drive, Gulf Breeze, Florida 32561</affiliation><affiliation>U.S. Environmental Protection Agency, National Health and Environmental Effects Research Laboratory, Gulf Ecology Division, 1 Sabine Island Drive, Gulf Breeze, Florida 32561</affiliation></author><author xml:id="author-2"><persName><forename type="first">Becky L.</forename><surname>Hemmer</surname></persName><affiliation>U.S. Environmental Protection Agency, National Health and Environmental Effects Research Laboratory, Gulf Ecology Division, 1 Sabine Island Drive, Gulf Breeze, Florida 32561</affiliation></author><author xml:id="author-3"><persName><forename type="first">Chris J.</forename><surname>Bowman</surname></persName><affiliation>Department of Biochemistry and Molecular Biology University of Florida, Gainesville, Florida 32610, USA</affiliation></author><author xml:id="author-4"><persName><forename type="first">Kevin J.</forename><surname>Kroll</surname></persName><affiliation>Department of Biochemistry and Molecular Biology University of Florida, Gainesville, Florida 32610, USA</affiliation></author><author xml:id="author-5"><persName><forename type="first">Leroy C</forename><surname>Folmar</surname></persName><affiliation>U.S. Environmental Protection Agency, National Health and Environmental Effects Research Laboratory, Gulf Ecology Division, 1 Sabine Island Drive, Gulf Breeze, Florida 32561</affiliation></author><author xml:id="author-6"><persName><forename type="first">Dragoslav</forename><surname>Marcovich</surname></persName><affiliation>U.S. Environmental Protection Agency, National Health and Environmental Effects Research Laboratory, Gulf Ecology Division, 1 Sabine Island Drive, Gulf Breeze, Florida 32561</affiliation></author><author xml:id="author-7"><persName><forename type="first">Marilynn D.</forename><surname>Hoglund</surname></persName><affiliation>U.S. Environmental Protection Agency, National Health and Environmental Effects Research Laboratory, Gulf Ecology Division, 1 Sabine Island Drive, Gulf Breeze, Florida 32561</affiliation></author><author xml:id="author-8"><persName><forename type="first">Nancy D.</forename><surname>Denslow</surname></persName><affiliation>Department of Biochemistry and Molecular Biology University of Florida, Gainesville, Florida 32610, USA</affiliation></author></analytic><monogr><title level="j">Environmental Toxicology and Chemistry</title><title level="j" type="sub">An International Journal</title><title level="j" type="abbrev">Environmental Toxicology and Chemistry</title><idno type="pISSN">0730-7268</idno><idno type="eISSN">1552-8618</idno><idno type="DOI">10.1002/(ISSN)1552-8618</idno><imprint><publisher>Wiley Periodicals, Inc.</publisher><pubPlace>Hoboken</pubPlace><date type="published" when="2001-02"></date><biblScope unit="volume">20</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="336">336</biblScope><biblScope unit="page" to="343">343</biblScope></imprint></monogr><idno type="istex">ECC4505F0A843E4FCE9D8C6B3718D3D1C26C5930</idno><idno type="DOI">10.1002/etc.5620200214</idno><idno type="ArticleID">ETC5620200214</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2001</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Temporal and dose‐responserelationshipsofvitellogenin(VTG) mRNA nductionand subsequent plasma VTG accumulation were established for sheepshead minnows (Cyprinodon variegatus) treated with p‐nonylphenol (an alkylphenol) and the organochlorine pesticides methoxychlor and endosulfan. Thirty‐two adult male fish per treatment were continuously exposed to measured concentrations of 0.64, 5.4, 11.8, 23.3, and 42.7 μg/L p‐nonylphenol; 1.1, 2.5, 5.6, 12.1, and 18.4 μg/L methoxychlor; and in two separate tests, 15.9, 36.3, 68.8, 162, 277, 403, 590, and 788 ng/L endosulfan using an intermittent flow‐through dosing apparatus. Separate triethylene glycol (50 (μl/L) and 17β‐estradiol (65.1 ng/L) treatments served as the negative and positive controls, respectively. Four fish were randomly sampled from each test concentration on days 2, 5, 13, 21, 35, and 42 of exposure, and levels of hepatic VTG mRNA induction and serum VTG accumulation were determined for each individual. Overall, fish exposed to p‐nonylphenol or methoxychlor demonstrated a rapid, dose‐dependent synthesis of VTG mRNA up to day 5 of exposure, followed by a relatively constant dose‐dependent expression through day 42. Both chemicals showed a dose‐dependent increase in plasma VTG over the entire time course of exposure, with significantly elevated VTG levels by the fifth day of exposure to p‐nonylphenol at concentrations of 5.4 μg/L or greater and to methoxychlor at concentrations of 2.5 μg/L or greater. Exposure to 0.64 μg/L p‐nonylphenol resulted in highly variable plasma VTG levels of less than 6 mg/ml. Exposures with endosulfan failed to induce measurable levels of either hepatic VTG mRNA or serum VTG at the chemical concentrations tested. Our results demonstrate that the sheepshead minnow bioassay is a suitable estuarine/marine teleost model for in vivo screening of potentially estrogenic substances.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>keywords</head><item><term>Fish</term></item><item><term>Vitellogenin</term></item><item><term>p‐Nonylphenol</term></item><item><term>Methoxychlor</term></item><item><term>Endosulfan</term></item></list></keywords></textClass><textClass><keywords scheme="Journal Subject"><list><head>article-category</head><item><term>Environmental Toxicology</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2000-02-15">Received</change><change when="2000-06-05">Registration</change><change 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Chemistry</title><title type="subtitle">An International Journal</title><title type="short">Environmental Toxicology and Chemistry</title></titleGroup></publicationMeta><publicationMeta level="part" position="20"><doi origin="wiley" registered="no">10.1002/etc.v20:2</doi><numberingGroup><numbering type="journalVolume" number="20">20</numbering><numbering type="journalIssue">2</numbering></numberingGroup><coverDate startDate="2001-02">February 2001</coverDate></publicationMeta><publicationMeta level="unit" type="article" position="14" status="forIssue"><doi origin="wiley" registered="no">10.1002/etc.5620200214</doi><idGroup><id type="unit" value="ETC5620200214"></id></idGroup><countGroup><count type="pageTotal" number="8"></count></countGroup><titleGroup><title type="articleCategory">Environmental Toxicology</title><title type="tocHeading1">Environmental Toxicology</title></titleGroup><copyright ownership="thirdParty">Copyright © 2001 SETAC</copyright><eventGroup><event type="manuscriptReceived" date="2000-02-15"></event><event type="manuscriptAccepted" date="2000-06-05"></event><event type="firstOnline" date="2009-11-03"></event><event type="publishedOnlineFinalForm" date="2009-11-03"></event><event type="xmlConverted" agent="Converter:JWSART34_TO_WML3G version:2.3.2 mode:FullText source:FullText result:FullText" date="2010-03-15"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:3.8.8" date="2014-01-25"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-10-16"></event></eventGroup><numberingGroup><numbering type="pageFirst">336</numbering><numbering type="pageLast">343</numbering></numberingGroup><correspondenceTo>U.S. Environmental Protection Agency, National Health and Environmental Effects Research Laboratory, Gulf Ecology Division, 1 Sabine Island Drive, Gulf Breeze, Florida 32561</correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:ETC.ETC5620200214.pdf"></link></linkGroup></publicationMeta><contentMeta><countGroup><count type="figureTotal" number="4"></count><count type="tableTotal" number="3"></count><count type="referenceTotal" number="48"></count></countGroup><titleGroup><title type="main" xml:lang="en">Effects of <i>p</i>‐nonylphenol, methoxychlor, and endosulfan on vitellogenin induction and expression in sheepshead minnow (<i>Cyprinodon variegatus</i>)<link href="#fn1"></link></title><title type="short" xml:lang="en">Vitellogenin induction in sheepshead minnow</title></titleGroup><creators><creator xml:id="au1" creatorRole="author" affiliationRef="#af1" corresponding="yes"><personName><givenNames>Michael J.</givenNames><familyName>Hemmer</familyName></personName><contactDetails><email>hemmer.michael@epa.gov</email></contactDetails></creator><creator xml:id="au2" creatorRole="author" affiliationRef="#af1"><personName><givenNames>Becky L.</givenNames><familyName>Hemmer</familyName></personName></creator><creator xml:id="au3" creatorRole="author" affiliationRef="#af2"><personName><givenNames>Chris J.</givenNames><familyName>Bowman</familyName></personName></creator><creator xml:id="au4" creatorRole="author" affiliationRef="#af2"><personName><givenNames>Kevin J.</givenNames><familyName>Kroll</familyName></personName></creator><creator xml:id="au5" creatorRole="author" affiliationRef="#af1"><personName><givenNames>Leroy C</givenNames><familyName>Folmar</familyName></personName></creator><creator xml:id="au6" creatorRole="author" affiliationRef="#af1"><personName><givenNames>Dragoslav</givenNames><familyName>Marcovich</familyName></personName></creator><creator xml:id="au7" creatorRole="author" affiliationRef="#af1"><personName><givenNames>Marilynn D.</givenNames><familyName>Hoglund</familyName></personName></creator><creator xml:id="au8" creatorRole="author" affiliationRef="#af2"><personName><givenNames>Nancy D.</givenNames><familyName>Denslow</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="af1" countryCode="US" type="organization"><unparsedAffiliation>U.S. Environmental Protection Agency, National Health and Environmental Effects Research Laboratory, Gulf Ecology Division, 1 Sabine Island Drive, Gulf Breeze, Florida 32561</unparsedAffiliation></affiliation><affiliation xml:id="af2" countryCode="US" type="organization"><unparsedAffiliation>Department of Biochemistry and Molecular Biology University of Florida, Gainesville, Florida 32610, USA</unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en" type="author"><keyword xml:id="kwd1">Fish</keyword><keyword xml:id="kwd2">Vitellogenin</keyword><keyword xml:id="kwd3"><i>p</i>‐Nonylphenol</keyword><keyword xml:id="kwd4">Methoxychlor</keyword><keyword xml:id="kwd5">Endosulfan</keyword></keywordGroup><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Abstract</title><p>Temporal and dose‐responserelationshipsofvitellogenin(VTG) mRNA nductionand subsequent plasma VTG accumulation were established for sheepshead minnows (<i>Cyprinodon variegatus</i>) treated with <i>p</i>‐nonylphenol (an alkylphenol) and the organochlorine pesticides methoxychlor and endosulfan. Thirty‐two adult male fish per treatment were continuously exposed to measured concentrations of 0.64, 5.4, 11.8, 23.3, and 42.7 μg/L <i>p</i>‐nonylphenol; 1.1, 2.5, 5.6, 12.1, and 18.4 μg/L methoxychlor; and in two separate tests, 15.9, 36.3, 68.8, 162, 277, 403, 590, and 788 ng/L endosulfan using an intermittent flow‐through dosing apparatus. Separate triethylene glycol (50 (μl/L) and 17β‐estradiol (65.1 ng/L) treatments served as the negative and positive controls, respectively. Four fish were randomly sampled from each test concentration on days 2, 5, 13, 21, 35, and 42 of exposure, and levels of hepatic VTG mRNA induction and serum VTG accumulation were determined for each individual. Overall, fish exposed to <i>p</i>‐nonylphenol or methoxychlor demonstrated a rapid, dose‐dependent synthesis of VTG mRNA up to day 5 of exposure, followed by a relatively constant dose‐dependent expression through day 42. Both chemicals showed a dose‐dependent increase in plasma VTG over the entire time course of exposure, with significantly elevated VTG levels by the fifth day of exposure to <i>p</i>‐nonylphenol at concentrations of 5.4 μg/L or greater and to methoxychlor at concentrations of 2.5 μg/L or greater. Exposure to 0.64 μg/L <i>p</i>‐nonylphenol resulted in highly variable plasma VTG levels of less than 6 mg/ml. Exposures with endosulfan failed to induce measurable levels of either hepatic VTG mRNA or serum VTG at the chemical concentrations tested. Our results demonstrate that the sheepshead minnow bioassay is a suitable estuarine/marine teleost model for in vivo screening of potentially estrogenic substances.</p></abstract></abstractGroup></contentMeta><noteGroup><note xml:id="fn1"><p>Contribution 1098, Gulf Ecology Division, Florida, USA. The contents of this document do not necessarily reflect the views or policies of the U.S. Environmental Protection Agency, nor does mention of trade names or commercial products constitute endorsement or recommendation for use.</p></note></noteGroup></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Effects of p‐nonylphenol, methoxychlor, and endosulfan on vitellogenin induction and expression in sheepshead minnow (Cyprinodon variegatus)</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>Vitellogenin induction in sheepshead minnow</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Effects of p‐nonylphenol, methoxychlor, and endosulfan on vitellogenin induction and expression in sheepshead minnow (Cyprinodon variegatus)</title></titleInfo><name type="personal"><namePart type="given">Michael J.</namePart><namePart type="family">Hemmer</namePart><affiliation>U.S. Environmental Protection Agency, National Health and Environmental Effects Research Laboratory, Gulf Ecology Division, 1 Sabine Island Drive, Gulf Breeze, Florida 32561</affiliation><affiliation>U.S. Environmental Protection Agency, National Health and Environmental Effects Research Laboratory, Gulf Ecology Division, 1 Sabine Island Drive, Gulf Breeze, Florida 32561</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Becky L.</namePart><namePart type="family">Hemmer</namePart><affiliation>U.S. Environmental Protection Agency, National Health and Environmental Effects Research Laboratory, Gulf Ecology Division, 1 Sabine Island Drive, Gulf Breeze, Florida 32561</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Chris J.</namePart><namePart type="family">Bowman</namePart><affiliation>Department of Biochemistry and Molecular Biology University of Florida, Gainesville, Florida 32610, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Kevin J.</namePart><namePart type="family">Kroll</namePart><affiliation>Department of Biochemistry and Molecular Biology University of Florida, Gainesville, Florida 32610, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Leroy C</namePart><namePart type="family">Folmar</namePart><affiliation>U.S. Environmental Protection Agency, National Health and Environmental Effects Research Laboratory, Gulf Ecology Division, 1 Sabine Island Drive, Gulf Breeze, Florida 32561</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Dragoslav</namePart><namePart type="family">Marcovich</namePart><affiliation>U.S. Environmental Protection Agency, National Health and Environmental Effects Research Laboratory, Gulf Ecology Division, 1 Sabine Island Drive, Gulf Breeze, Florida 32561</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Marilynn D.</namePart><namePart type="family">Hoglund</namePart><affiliation>U.S. Environmental Protection Agency, National Health and Environmental Effects Research Laboratory, Gulf Ecology Division, 1 Sabine Island Drive, Gulf Breeze, Florida 32561</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Nancy D.</namePart><namePart type="family">Denslow</namePart><affiliation>Department of Biochemistry and Molecular Biology University of Florida, Gainesville, Florida 32610, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Wiley Periodicals, Inc.</publisher><place><placeTerm type="text">Hoboken</placeTerm></place><dateIssued encoding="w3cdtf">2001-02</dateIssued><dateCaptured encoding="w3cdtf">2000-02-15</dateCaptured><dateValid encoding="w3cdtf">2000-06-05</dateValid><copyrightDate encoding="w3cdtf">2001</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">4</extent><extent unit="tables">3</extent><extent unit="references">48</extent></physicalDescription><abstract lang="en">Temporal and dose‐responserelationshipsofvitellogenin(VTG) mRNA nductionand subsequent plasma VTG accumulation were established for sheepshead minnows (Cyprinodon variegatus) treated with p‐nonylphenol (an alkylphenol) and the organochlorine pesticides methoxychlor and endosulfan. Thirty‐two adult male fish per treatment were continuously exposed to measured concentrations of 0.64, 5.4, 11.8, 23.3, and 42.7 μg/L p‐nonylphenol; 1.1, 2.5, 5.6, 12.1, and 18.4 μg/L methoxychlor; and in two separate tests, 15.9, 36.3, 68.8, 162, 277, 403, 590, and 788 ng/L endosulfan using an intermittent flow‐through dosing apparatus. Separate triethylene glycol (50 (μl/L) and 17β‐estradiol (65.1 ng/L) treatments served as the negative and positive controls, respectively. Four fish were randomly sampled from each test concentration on days 2, 5, 13, 21, 35, and 42 of exposure, and levels of hepatic VTG mRNA induction and serum VTG accumulation were determined for each individual. Overall, fish exposed to p‐nonylphenol or methoxychlor demonstrated a rapid, dose‐dependent synthesis of VTG mRNA up to day 5 of exposure, followed by a relatively constant dose‐dependent expression through day 42. Both chemicals showed a dose‐dependent increase in plasma VTG over the entire time course of exposure, with significantly elevated VTG levels by the fifth day of exposure to p‐nonylphenol at concentrations of 5.4 μg/L or greater and to methoxychlor at concentrations of 2.5 μg/L or greater. Exposure to 0.64 μg/L p‐nonylphenol resulted in highly variable plasma VTG levels of less than 6 mg/ml. Exposures with endosulfan failed to induce measurable levels of either hepatic VTG mRNA or serum VTG at the chemical concentrations tested. Our results demonstrate that the sheepshead minnow bioassay is a suitable estuarine/marine teleost model for in vivo screening of potentially estrogenic substances.</abstract><note type="content">*Contribution 1098, Gulf Ecology Division, Florida, USA. The contents of this document do not necessarily reflect the views or policies of the U.S. Environmental Protection Agency, nor does mention of trade names or commercial products constitute endorsement or recommendation for use.</note><subject lang="en"><genre>keywords</genre><topic>Fish</topic><topic>Vitellogenin</topic><topic>p‐Nonylphenol</topic><topic>Methoxychlor</topic><topic>Endosulfan</topic></subject><relatedItem type="host"><titleInfo><title>Environmental Toxicology and Chemistry</title><subTitle>An International Journal</subTitle></titleInfo><titleInfo type="abbreviated"><title>Environmental Toxicology and Chemistry</title></titleInfo><genre type="journal">journal</genre><subject><genre>article-category</genre><topic>Environmental Toxicology</topic></subject><identifier type="ISSN">0730-7268</identifier><identifier type="eISSN">1552-8618</identifier><identifier type="DOI">10.1002/(ISSN)1552-8618</identifier><identifier type="PublisherID">ETC</identifier><part><date>2001</date><detail type="volume"><caption>vol.</caption><number>20</number></detail><detail type="issue"><caption>no.</caption><number>2</number></detail><extent unit="pages"><start>336</start><end>343</end><total>8</total></extent></part></relatedItem><identifier type="istex">ECC4505F0A843E4FCE9D8C6B3718D3D1C26C5930</identifier><identifier type="DOI">10.1002/etc.5620200214</identifier><identifier type="ArticleID">ETC5620200214</identifier><accessCondition type="use and reproduction" contentType="copyright">Copyright © 2001 SETAC</accessCondition><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>Wiley Periodicals, Inc.</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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