Cholinergic elements in the zebrafish central nervous system: Histochemical and immunohistochemical analysis
Identifieur interne : 001406 ( Istex/Corpus ); précédent : 001405; suivant : 001407Cholinergic elements in the zebrafish central nervous system: Histochemical and immunohistochemical analysis
Auteurs : Diego Clemente ; Ángel Porteros ; Eduardo Weruaga ; José Ram N Alonso ; Francisco Javier Arenzana ; José Aij N ; Rosario ArévaloSource :
- Journal of Comparative Neurology [ 0021-9967 ] ; 2004-06-14.
English descriptors
- KwdEn :
Abstract
Recently, the zebrafish has been extensively used for studying the development of the central nervous system (CNS). However, the zebrafish CNS has been poorly analyzed in the adult. The cholinergic/cholinoceptive system of the zebrafish CNS was analyzed by using choline acetyltransferase (ChAT) immunohistochemistry and acetylcholinesterase (AChE) histochemistry in the brain, retina, and spinal cord. AChE labeling was more abundant and more widely distributed than ChAT immunoreactivity. In the telencephalon, ChAT‐immunoreactive (ChAT‐ir) cells were absent, whereas AChE‐positive neurons were observed in both the olfactory bulb and the telencephalic hemispheres. The diencephalon was the region with the lowest density of AChE‐positive cells, mainly located in the pretectum, whereas ChAT‐ir cells were exclusively located in the preoptic region. ChAT‐ir cells were restricted to the periventricular stratum of the optic tectum, but AChE‐positive neurons were observed throughout the whole extension of the lamination except in the marginal stratum. Although ChAT immunoreactivity was restricted to the rostral tegmental, oculomotor, and trochlear nuclei within the mesencephalic tegmentum, a widespread distribution of AChE reactivity was observed in this region. The isthmic region showed abundant AChE‐positive and ChAT‐ir cells in the isthmic, secondary gustatory and superior reticular nucleus and in the nucleus lateralis valvulae. ChAT immunoreactivity was absent in the cerebellum, although AChE staining was observed in Purkinje and granule cells. The medulla oblongata showed a widespread distribution of AChE‐positive cells in all main subdivisions, including the octavolateral area, reticular formation, and motor nuclei of the cranial nerves. ChAT‐ir elements in this area were restricted to the descending octaval nucleus, the octaval efferent nucleus and the motor nuclei of the cranial nerves. Additionally, spinal cord motoneurons appeared positive to both markers. Substantial differences in the ChAT and AChE distribution between zebrafish and other fish species were observed, which could be important because zebrafish is widely used as a genetic or developmental animal model. J. Comp. Neurol. 474:75–107, 2004. © 2004 Wiley‐Liss, Inc.
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DOI: 10.1002/cne.20111
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Although ChAT immunoreactivity was restricted to the rostral tegmental, oculomotor, and trochlear nuclei within the mesencephalic tegmentum, a widespread distribution of AChE reactivity was observed in this region. The isthmic region showed abundant AChE‐positive and ChAT‐ir cells in the isthmic, secondary gustatory and superior reticular nucleus and in the nucleus lateralis valvulae. ChAT immunoreactivity was absent in the cerebellum, although AChE staining was observed in Purkinje and granule cells. The medulla oblongata showed a widespread distribution of AChE‐positive cells in all main subdivisions, including the octavolateral area, reticular formation, and motor nuclei of the cranial nerves. ChAT‐ir elements in this area were restricted to the descending octaval nucleus, the octaval efferent nucleus and the motor nuclei of the cranial nerves. Additionally, spinal cord motoneurons appeared positive to both markers. Substantial differences in the ChAT and AChE distribution between zebrafish and other fish species were observed, which could be important because zebrafish is widely used as a genetic or developmental animal model. J. Comp. Neurol. 474:75–107, 2004. © 2004 Wiley‐Liss, Inc.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>Diego Clemente</name><affiliations><json:string>Departamento de Biología Celular y Patología, Instituto de Neurociencias de Castilla y León, Universidad de Salamanca, E‐37007 Salamanca, Spain</json:string></affiliations></json:item><json:item><name>Ángel Porteros</name><affiliations><json:string>Departamento de Biología Celular y Patología, Instituto de Neurociencias de Castilla y León, Universidad de Salamanca, E‐37007 Salamanca, Spain</json:string></affiliations></json:item><json:item><name>Eduardo Weruaga</name><affiliations><json:string>Departamento de Biología Celular y Patología, Instituto de Neurociencias de Castilla y León, Universidad de Salamanca, E‐37007 Salamanca, Spain</json:string></affiliations></json:item><json:item><name>José Ramón Alonso</name><affiliations><json:string>Departamento de Biología Celular y Patología, Instituto de Neurociencias de Castilla y León, Universidad de Salamanca, E‐37007 Salamanca, Spain</json:string></affiliations></json:item><json:item><name>Francisco Javier Arenzana</name><affiliations><json:string>Departamento de Biología Celular y Patología, Instituto de Neurociencias de Castilla y León, Universidad de Salamanca, E‐37007 Salamanca, Spain</json:string></affiliations></json:item><json:item><name>José Aijón</name><affiliations><json:string>Departamento de Biología Celular y Patología, Instituto de Neurociencias de Castilla y León, Universidad de Salamanca, E‐37007 Salamanca, Spain</json:string></affiliations></json:item><json:item><name>Rosario Arévalo</name><affiliations><json:string>Departamento de Biología Celular y Patología, Instituto de Neurociencias de Castilla y León, Universidad de Salamanca, E‐37007 Salamanca, Spain</json:string><json:string>Dpto. de Biología Celular y Patología, Universidad de Salamanca, Facultad de Medicina, Campus Miguel de Unamuno, c/Alfonso X el Sabio, 1, E‐37007 Salamanca, Spain</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>acetylcholine</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>cholinergic</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>cholinoceptive</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>teleost</value></json:item></subject><articleId><json:string>CNE20111</json:string></articleId><language><json:string>eng</json:string></language><originalGenre><json:string>article</json:string></originalGenre><abstract>Recently, the zebrafish has been extensively used for studying the development of the central nervous system (CNS). However, the zebrafish CNS has been poorly analyzed in the adult. The cholinergic/cholinoceptive system of the zebrafish CNS was analyzed by using choline acetyltransferase (ChAT) immunohistochemistry and acetylcholinesterase (AChE) histochemistry in the brain, retina, and spinal cord. AChE labeling was more abundant and more widely distributed than ChAT immunoreactivity. In the telencephalon, ChAT‐immunoreactive (ChAT‐ir) cells were absent, whereas AChE‐positive neurons were observed in both the olfactory bulb and the telencephalic hemispheres. The diencephalon was the region with the lowest density of AChE‐positive cells, mainly located in the pretectum, whereas ChAT‐ir cells were exclusively located in the preoptic region. ChAT‐ir cells were restricted to the periventricular stratum of the optic tectum, but AChE‐positive neurons were observed throughout the whole extension of the lamination except in the marginal stratum. Although ChAT immunoreactivity was restricted to the rostral tegmental, oculomotor, and trochlear nuclei within the mesencephalic tegmentum, a widespread distribution of AChE reactivity was observed in this region. The isthmic region showed abundant AChE‐positive and ChAT‐ir cells in the isthmic, secondary gustatory and superior reticular nucleus and in the nucleus lateralis valvulae. ChAT immunoreactivity was absent in the cerebellum, although AChE staining was observed in Purkinje and granule cells. The medulla oblongata showed a widespread distribution of AChE‐positive cells in all main subdivisions, including the octavolateral area, reticular formation, and motor nuclei of the cranial nerves. ChAT‐ir elements in this area were restricted to the descending octaval nucleus, the octaval efferent nucleus and the motor nuclei of the cranial nerves. Additionally, spinal cord motoneurons appeared positive to both markers. Substantial differences in the ChAT and AChE distribution between zebrafish and other fish species were observed, which could be important because zebrafish is widely used as a genetic or developmental animal model. J. Comp. 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The cholinergic/cholinoceptive system of the zebrafish CNS was analyzed by using choline acetyltransferase (ChAT) immunohistochemistry and acetylcholinesterase (AChE) histochemistry in the brain, retina, and spinal cord. AChE labeling was more abundant and more widely distributed than ChAT immunoreactivity. In the telencephalon, ChAT‐immunoreactive (ChAT‐ir) cells were absent, whereas AChE‐positive neurons were observed in both the olfactory bulb and the telencephalic hemispheres. The diencephalon was the region with the lowest density of AChE‐positive cells, mainly located in the pretectum, whereas ChAT‐ir cells were exclusively located in the preoptic region. ChAT‐ir cells were restricted to the periventricular stratum of the optic tectum, but AChE‐positive neurons were observed throughout the whole extension of the lamination except in the marginal stratum. Although ChAT immunoreactivity was restricted to the rostral tegmental, oculomotor, and trochlear nuclei within the mesencephalic tegmentum, a widespread distribution of AChE reactivity was observed in this region. The isthmic region showed abundant AChE‐positive and ChAT‐ir cells in the isthmic, secondary gustatory and superior reticular nucleus and in the nucleus lateralis valvulae. ChAT immunoreactivity was absent in the cerebellum, although AChE staining was observed in Purkinje and granule cells. The medulla oblongata showed a widespread distribution of AChE‐positive cells in all main subdivisions, including the octavolateral area, reticular formation, and motor nuclei of the cranial nerves. ChAT‐ir elements in this area were restricted to the descending octaval nucleus, the octaval efferent nucleus and the motor nuclei of the cranial nerves. Additionally, spinal cord motoneurons appeared positive to both markers. Substantial differences in the ChAT and AChE distribution between zebrafish and other fish species were observed, which could be important because zebrafish is widely used as a genetic or developmental animal model. J. Comp. Neurol. 474:75–107, 2004. © 2004 Wiley‐Liss, Inc.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>keywords</head><item><term>acetylcholine</term></item><item><term>cholinergic</term></item><item><term>cholinoceptive</term></item><item><term>teleost</term></item></list></keywords></textClass><textClass><keywords scheme="Journal Subject"><list><head>article-category</head><item><term>Article</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2003-08-06">Received</change><change when="2004-01-26">Registration</change><change when="2004-06-14">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/A40D84C44F8BBF0F8471F9C892E0B3790628DB34/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>Wiley Subscription Services, Inc., A Wiley Company</publisherName><publisherLoc>Hoboken</publisherLoc></publisherInfo><doi registered="yes">10.1002/(ISSN)1096-9861</doi><issn type="print">0021-9967</issn><issn type="electronic">1096-9861</issn><idGroup><id type="product" value="CNE"></id></idGroup><titleGroup><title type="main" xml:lang="en">Journal of Comparative Neurology</title><title type="short">J. 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Neurol.</title></titleGroup></publicationMeta><publicationMeta level="part" position="10"><doi origin="wiley" registered="yes">10.1002/cne.v474:1</doi><numberingGroup><numbering type="journalVolume" number="474">474</numbering><numbering type="journalIssue">1</numbering></numberingGroup><coverDate startDate="2004-06-14">14 June 2004</coverDate></publicationMeta><publicationMeta level="unit" type="article" position="60" status="forIssue"><doi origin="wiley" registered="yes">10.1002/cne.20111</doi><idGroup><id type="unit" value="CNE20111"></id></idGroup><countGroup><count type="pageTotal" number="33"></count></countGroup><titleGroup><title type="articleCategory">Article</title><title type="tocHeading1">Article</title></titleGroup><copyright ownership="publisher">Copyright © 2004 Wiley‐Liss, Inc.</copyright><eventGroup><event type="manuscriptReceived" date="2003-08-06"></event><event type="manuscriptRevised" date="2003-11-25"></event><event type="manuscriptAccepted" date="2004-01-26"></event><event type="firstOnline" date="2004-05-17"></event><event type="publishedOnlineFinalForm" date="2004-05-17"></event><event type="xmlConverted" agent="Converter:JWSART34_TO_WML3G version:2.3.2 mode:FullText source:FullText result:FullText" date="2010-03-01"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:3.8.8" date="2014-01-15"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-10-30"></event></eventGroup><numberingGroup><numbering type="pageFirst">75</numbering><numbering type="pageLast">107</numbering></numberingGroup><correspondenceTo>Dpto. de Biología Celular y Patología, Universidad de Salamanca, Facultad de Medicina, Campus Miguel de Unamuno, c/Alfonso X el Sabio, 1, E‐37007 Salamanca, Spain</correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:CNE.CNE20111.pdf"></link></linkGroup></publicationMeta><contentMeta><countGroup><count type="figureTotal" number="12"></count><count type="tableTotal" number="1"></count><count type="referenceTotal" number="172"></count><count type="wordTotal" number="21331"></count></countGroup><titleGroup><title type="main" xml:lang="en">Cholinergic elements in the zebrafish central nervous system: Histochemical and immunohistochemical analysis</title><title type="short" xml:lang="en">Zebrafish Cholinergic System</title></titleGroup><creators><creator xml:id="au1" creatorRole="author" affiliationRef="#af1"><personName><givenNames>Diego</givenNames><familyName>Clemente</familyName></personName></creator><creator xml:id="au2" creatorRole="author" affiliationRef="#af1"><personName><givenNames>Ángel</givenNames><familyName>Porteros</familyName></personName></creator><creator xml:id="au3" creatorRole="author" affiliationRef="#af1"><personName><givenNames>Eduardo</givenNames><familyName>Weruaga</familyName></personName></creator><creator xml:id="au4" creatorRole="author" affiliationRef="#af1"><personName><givenNames>José Ramón</givenNames><familyName>Alonso</familyName></personName></creator><creator xml:id="au5" creatorRole="author" affiliationRef="#af1"><personName><givenNames>Francisco Javier</givenNames><familyName>Arenzana</familyName></personName></creator><creator xml:id="au6" creatorRole="author" affiliationRef="#af1"><personName><givenNames>José</givenNames><familyName>Aijón</familyName></personName></creator><creator xml:id="au7" creatorRole="author" affiliationRef="#af1" corresponding="yes"><personName><givenNames>Rosario</givenNames><familyName>Arévalo</familyName></personName><contactDetails><email>mraa@usal.es</email></contactDetails></creator></creators><affiliationGroup><affiliation xml:id="af1" countryCode="ES" type="organization"><unparsedAffiliation>Departamento de Biología Celular y Patología, Instituto de Neurociencias de Castilla y León, Universidad de Salamanca, E‐37007 Salamanca, Spain</unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en" type="author"><keyword xml:id="kwd1">acetylcholine</keyword><keyword xml:id="kwd2">cholinergic</keyword><keyword xml:id="kwd3">cholinoceptive</keyword><keyword xml:id="kwd4">teleost</keyword></keywordGroup><fundingInfo><fundingAgency>Spanish Fondo de Investigaciones Sanitarias</fundingAgency><fundingNumber>PI021730</fundingNumber></fundingInfo><fundingInfo><fundingAgency>Junta de Castilla y León</fundingAgency></fundingInfo><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Abstract</title><p>Recently, the zebrafish has been extensively used for studying the development of the central nervous system (CNS). However, the zebrafish CNS has been poorly analyzed in the adult. The cholinergic/cholinoceptive system of the zebrafish CNS was analyzed by using choline acetyltransferase (ChAT) immunohistochemistry and acetylcholinesterase (AChE) histochemistry in the brain, retina, and spinal cord. AChE labeling was more abundant and more widely distributed than ChAT immunoreactivity. In the telencephalon, ChAT‐immunoreactive (ChAT‐ir) cells were absent, whereas AChE‐positive neurons were observed in both the olfactory bulb and the telencephalic hemispheres. The diencephalon was the region with the lowest density of AChE‐positive cells, mainly located in the pretectum, whereas ChAT‐ir cells were exclusively located in the preoptic region. ChAT‐ir cells were restricted to the periventricular stratum of the optic tectum, but AChE‐positive neurons were observed throughout the whole extension of the lamination except in the marginal stratum. Although ChAT immunoreactivity was restricted to the rostral tegmental, oculomotor, and trochlear nuclei within the mesencephalic tegmentum, a widespread distribution of AChE reactivity was observed in this region. The isthmic region showed abundant AChE‐positive and ChAT‐ir cells in the isthmic, secondary gustatory and superior reticular nucleus and in the nucleus lateralis valvulae. ChAT immunoreactivity was absent in the cerebellum, although AChE staining was observed in Purkinje and granule cells. The medulla oblongata showed a widespread distribution of AChE‐positive cells in all main subdivisions, including the octavolateral area, reticular formation, and motor nuclei of the cranial nerves. ChAT‐ir elements in this area were restricted to the descending octaval nucleus, the octaval efferent nucleus and the motor nuclei of the cranial nerves. Additionally, spinal cord motoneurons appeared positive to both markers. Substantial differences in the ChAT and AChE distribution between zebrafish and other fish species were observed, which could be important because zebrafish is widely used as a genetic or developmental animal model. J. Comp. Neurol. 474:75–107, 2004. © 2004 Wiley‐Liss, Inc.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Cholinergic elements in the zebrafish central nervous system: Histochemical and immunohistochemical analysis</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>Zebrafish Cholinergic System</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Cholinergic elements in the zebrafish central nervous system: Histochemical and immunohistochemical analysis</title></titleInfo><name type="personal"><namePart type="given">Diego</namePart><namePart type="family">Clemente</namePart><affiliation>Departamento de Biología Celular y Patología, Instituto de Neurociencias de Castilla y León, Universidad de Salamanca, E‐37007 Salamanca, Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Ángel</namePart><namePart type="family">Porteros</namePart><affiliation>Departamento de Biología Celular y Patología, Instituto de Neurociencias de Castilla y León, Universidad de Salamanca, E‐37007 Salamanca, Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Eduardo</namePart><namePart type="family">Weruaga</namePart><affiliation>Departamento de Biología Celular y Patología, Instituto de Neurociencias de Castilla y León, Universidad de Salamanca, E‐37007 Salamanca, Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">José Ramón</namePart><namePart type="family">Alonso</namePart><affiliation>Departamento de Biología Celular y Patología, Instituto de Neurociencias de Castilla y León, Universidad de Salamanca, E‐37007 Salamanca, Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Francisco Javier</namePart><namePart type="family">Arenzana</namePart><affiliation>Departamento de Biología Celular y Patología, Instituto de Neurociencias de Castilla y León, Universidad de Salamanca, E‐37007 Salamanca, Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">José</namePart><namePart type="family">Aijón</namePart><affiliation>Departamento de Biología Celular y Patología, Instituto de Neurociencias de Castilla y León, Universidad de Salamanca, E‐37007 Salamanca, Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Rosario</namePart><namePart type="family">Arévalo</namePart><affiliation>Departamento de Biología Celular y Patología, Instituto de Neurociencias de Castilla y León, Universidad de Salamanca, E‐37007 Salamanca, Spain</affiliation><affiliation>Dpto. de Biología Celular y Patología, Universidad de Salamanca, Facultad de Medicina, Campus Miguel de Unamuno, c/Alfonso X el Sabio, 1, E‐37007 Salamanca, Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><place><placeTerm type="text">Hoboken</placeTerm></place><dateIssued encoding="w3cdtf">2004-06-14</dateIssued><dateCaptured encoding="w3cdtf">2003-08-06</dateCaptured><dateValid encoding="w3cdtf">2004-01-26</dateValid><copyrightDate encoding="w3cdtf">2004</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">12</extent><extent unit="tables">1</extent><extent unit="references">172</extent><extent unit="words">21331</extent></physicalDescription><abstract lang="en">Recently, the zebrafish has been extensively used for studying the development of the central nervous system (CNS). However, the zebrafish CNS has been poorly analyzed in the adult. The cholinergic/cholinoceptive system of the zebrafish CNS was analyzed by using choline acetyltransferase (ChAT) immunohistochemistry and acetylcholinesterase (AChE) histochemistry in the brain, retina, and spinal cord. AChE labeling was more abundant and more widely distributed than ChAT immunoreactivity. In the telencephalon, ChAT‐immunoreactive (ChAT‐ir) cells were absent, whereas AChE‐positive neurons were observed in both the olfactory bulb and the telencephalic hemispheres. The diencephalon was the region with the lowest density of AChE‐positive cells, mainly located in the pretectum, whereas ChAT‐ir cells were exclusively located in the preoptic region. ChAT‐ir cells were restricted to the periventricular stratum of the optic tectum, but AChE‐positive neurons were observed throughout the whole extension of the lamination except in the marginal stratum. Although ChAT immunoreactivity was restricted to the rostral tegmental, oculomotor, and trochlear nuclei within the mesencephalic tegmentum, a widespread distribution of AChE reactivity was observed in this region. The isthmic region showed abundant AChE‐positive and ChAT‐ir cells in the isthmic, secondary gustatory and superior reticular nucleus and in the nucleus lateralis valvulae. ChAT immunoreactivity was absent in the cerebellum, although AChE staining was observed in Purkinje and granule cells. The medulla oblongata showed a widespread distribution of AChE‐positive cells in all main subdivisions, including the octavolateral area, reticular formation, and motor nuclei of the cranial nerves. ChAT‐ir elements in this area were restricted to the descending octaval nucleus, the octaval efferent nucleus and the motor nuclei of the cranial nerves. Additionally, spinal cord motoneurons appeared positive to both markers. Substantial differences in the ChAT and AChE distribution between zebrafish and other fish species were observed, which could be important because zebrafish is widely used as a genetic or developmental animal model. J. Comp. Neurol. 474:75–107, 2004. © 2004 Wiley‐Liss, Inc.</abstract><note type="funding">Spanish Fondo de Investigaciones Sanitarias - No. PI021730; </note><note type="funding">Junta de Castilla y León</note><subject lang="en"><genre>keywords</genre><topic>acetylcholine</topic><topic>cholinergic</topic><topic>cholinoceptive</topic><topic>teleost</topic></subject><relatedItem type="host"><titleInfo><title>Journal of Comparative Neurology</title></titleInfo><titleInfo type="abbreviated"><title>J. Comp. Neurol.</title></titleInfo><genre type="journal">journal</genre><subject><genre>article-category</genre><topic>Article</topic></subject><identifier type="ISSN">0021-9967</identifier><identifier type="eISSN">1096-9861</identifier><identifier type="DOI">10.1002/(ISSN)1096-9861</identifier><identifier type="PublisherID">CNE</identifier><part><date>2004</date><detail type="volume"><caption>vol.</caption><number>474</number></detail><detail type="issue"><caption>no.</caption><number>1</number></detail><extent unit="pages"><start>75</start><end>107</end><total>33</total></extent></part></relatedItem><identifier type="istex">A40D84C44F8BBF0F8471F9C892E0B3790628DB34</identifier><identifier type="DOI">10.1002/cne.20111</identifier><identifier type="ArticleID">CNE20111</identifier><accessCondition type="use and reproduction" contentType="copyright">Copyright © 2004 Wiley‐Liss, Inc.</accessCondition><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>Wiley Subscription Services, Inc., A Wiley Company</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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