Determination and multivariate statistical analysis of biochemical responses to environmental contaminants in feral freshwater fish Leuciscus cephalus L.
Identifieur interne : 001404 ( Istex/Corpus ); précédent : 001403; suivant : 001405Determination and multivariate statistical analysis of biochemical responses to environmental contaminants in feral freshwater fish Leuciscus cephalus L.
Auteurs : Miroslav Machala ; Ladislav Dusek ; Klara Hilscherova ; Renata Kubinova ; Pavel Jurajda ; Jiri Neca ; Robert Ulrich ; Milan Gelnar ; Zdena Studnickova ; Ivan HoloubekSource :
- Environmental Toxicology and Chemistry [ 0730-7268 ] ; 2001-05.
English descriptors
- KwdEn :
Abstract
Modulations of 11 prospective biochemical markers of impacts of aquatic pollutants in liver tissue of chub (Leuciscus cephalus), caught at several sampling sites of a river with various pollution types and rates, were matched against analytical data of concentrations of organochlorine compounds, polycyclic aromatic hydrocarbons (PAHs), and heavy metals. Multivariate principal component analysis (PCA) of the field data showed general patterns of biochemical responses to different types of pollutants and relationships among the biomarkers. Cytochrome P4501A‐dependent 7‐ethoxyresorufin O‐deethylase (EROD) activity, inducible by 2,3,7,8‐tetrachlorodibenzo‐p‐dioxin and structurally related planar compounds, was strongly enhanced in the more contaminated areas. Compared with polychlorinated aromatic hydrocarbons, PAHs did not contribute so significantly to EROD induction. Testosterone 6β‐ and 16α‐hydroxylase activities, as an expression of the cytochrome P4503A27, were slightly increased at several sites but were significantly decreased in samples from some heavily polluted areas. Recently, these activities have been suggested as potential biomarkers of exposure to contaminants that do not induce cytochrome P4501A. In this study, their inhibition or induction was not associated with a specific class of monitored contaminants, and selectivities of these modulations are still to be investigated. Similar modulations of the prospective biochemical indicators of oxidative stress, including microsomal glutathione S‐transferase activity, cytosolic glutathione S‐transferase with ethacrynic acid, and glutathione reductase, were demonstrated by PCA. The pattern of the modulations of the microsomal nicotinamide adenine dinucleotide phosphate (NADPH)‐dependent lipid peroxidation in vitro differed from the responses of the rest of oxidative stress parameters at some sampling sites. Further biochemical markers of oxidative stress under study, including in vivo lipid peroxidation, in vitro production of reactive oxygen species, and the concentration of metallothioneins did not correlate well with the concentrations of the contaminants. Principal component analysis demonstrated that the EROD activity, glutathione‐dependent enzymes, and Fe(II)‐enhanced lipid peroxidation formed a suitable battery of biomarkers of exposure.
Url:
DOI: 10.1002/etc.5620200528
Links to Exploration step
ISTEX:3AE8F545906E07F2F216543FDEE8B60B8939F4E7Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Determination and multivariate statistical analysis of biochemical responses to environmental contaminants in feral freshwater fish Leuciscus cephalus L.</title>
<author><name sortKey="Machala, Miroslav" sort="Machala, Miroslav" uniqKey="Machala M" first="Miroslav" last="Machala">Miroslav Machala</name>
<affiliation><mods:affiliation>Veterinary Research Institute, 62132 Brno, Czech Republic</mods:affiliation>
</affiliation>
<affiliation><mods:affiliation>Veterinary Research Institute, 62132 Brno, Czech Republic</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Dusek, Ladislav" sort="Dusek, Ladislav" uniqKey="Dusek L" first="Ladislav" last="Dusek">Ladislav Dusek</name>
<affiliation><mods:affiliation>Masaryk University, Faculty of Science 61137 Brno Czech Republic</mods:affiliation>
</affiliation>
<affiliation><mods:affiliation>RECETOX‐TOCOEN, 61137 Brno, Czech Republic</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Hilscherova, Klara" sort="Hilscherova, Klara" uniqKey="Hilscherova K" first="Klara" last="Hilscherova">Klara Hilscherova</name>
<affiliation><mods:affiliation>Masaryk University, Faculty of Science 61137 Brno Czech Republic</mods:affiliation>
</affiliation>
<affiliation><mods:affiliation>RECETOX‐TOCOEN, 61137 Brno, Czech Republic</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Kubinova, Renata" sort="Kubinova, Renata" uniqKey="Kubinova R" first="Renata" last="Kubinova">Renata Kubinova</name>
<affiliation><mods:affiliation>Veterinary and Pharmaceutical University, 61200 Brno, Czech Republic</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Jurajda, Pavel" sort="Jurajda, Pavel" uniqKey="Jurajda P" first="Pavel" last="Jurajda">Pavel Jurajda</name>
<affiliation><mods:affiliation>Landscape Ecology Institute, Academy of Sciences of the Czech Republic, Brno, Czech Republic</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Neca, Jiri" sort="Neca, Jiri" uniqKey="Neca J" first="Jiri" last="Neca">Jiri Neca</name>
<affiliation><mods:affiliation>Veterinary Research Institute, 62132 Brno, Czech Republic</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Ulrich, Robert" sort="Ulrich, Robert" uniqKey="Ulrich R" first="Robert" last="Ulrich">Robert Ulrich</name>
<affiliation><mods:affiliation>Veterinary Research Institute, 62132 Brno, Czech Republic</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Gelnar, Milan" sort="Gelnar, Milan" uniqKey="Gelnar M" first="Milan" last="Gelnar">Milan Gelnar</name>
<affiliation><mods:affiliation>Masaryk University, Faculty of Science 61137 Brno Czech Republic</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Studnickova, Zdena" sort="Studnickova, Zdena" uniqKey="Studnickova Z" first="Zdena" last="Studnickova">Zdena Studnickova</name>
<affiliation><mods:affiliation>Masaryk University, Faculty of Science 61137 Brno Czech Republic</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Holoubek, Ivan" sort="Holoubek, Ivan" uniqKey="Holoubek I" first="Ivan" last="Holoubek">Ivan Holoubek</name>
<affiliation><mods:affiliation>Masaryk University, Faculty of Science 61137 Brno Czech Republic</mods:affiliation>
</affiliation>
<affiliation><mods:affiliation>RECETOX‐TOCOEN, 61137 Brno, Czech Republic</mods:affiliation>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:3AE8F545906E07F2F216543FDEE8B60B8939F4E7</idno>
<date when="2001" year="2001">2001</date>
<idno type="doi">10.1002/etc.5620200528</idno>
<idno type="url">https://api.istex.fr/document/3AE8F545906E07F2F216543FDEE8B60B8939F4E7/fulltext/pdf</idno>
<idno type="wicri:Area/Istex/Corpus">001404</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001404</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Determination and multivariate statistical analysis of biochemical responses to environmental contaminants in feral freshwater fish Leuciscus cephalus L.</title>
<author><name sortKey="Machala, Miroslav" sort="Machala, Miroslav" uniqKey="Machala M" first="Miroslav" last="Machala">Miroslav Machala</name>
<affiliation><mods:affiliation>Veterinary Research Institute, 62132 Brno, Czech Republic</mods:affiliation>
</affiliation>
<affiliation><mods:affiliation>Veterinary Research Institute, 62132 Brno, Czech Republic</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Dusek, Ladislav" sort="Dusek, Ladislav" uniqKey="Dusek L" first="Ladislav" last="Dusek">Ladislav Dusek</name>
<affiliation><mods:affiliation>Masaryk University, Faculty of Science 61137 Brno Czech Republic</mods:affiliation>
</affiliation>
<affiliation><mods:affiliation>RECETOX‐TOCOEN, 61137 Brno, Czech Republic</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Hilscherova, Klara" sort="Hilscherova, Klara" uniqKey="Hilscherova K" first="Klara" last="Hilscherova">Klara Hilscherova</name>
<affiliation><mods:affiliation>Masaryk University, Faculty of Science 61137 Brno Czech Republic</mods:affiliation>
</affiliation>
<affiliation><mods:affiliation>RECETOX‐TOCOEN, 61137 Brno, Czech Republic</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Kubinova, Renata" sort="Kubinova, Renata" uniqKey="Kubinova R" first="Renata" last="Kubinova">Renata Kubinova</name>
<affiliation><mods:affiliation>Veterinary and Pharmaceutical University, 61200 Brno, Czech Republic</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Jurajda, Pavel" sort="Jurajda, Pavel" uniqKey="Jurajda P" first="Pavel" last="Jurajda">Pavel Jurajda</name>
<affiliation><mods:affiliation>Landscape Ecology Institute, Academy of Sciences of the Czech Republic, Brno, Czech Republic</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Neca, Jiri" sort="Neca, Jiri" uniqKey="Neca J" first="Jiri" last="Neca">Jiri Neca</name>
<affiliation><mods:affiliation>Veterinary Research Institute, 62132 Brno, Czech Republic</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Ulrich, Robert" sort="Ulrich, Robert" uniqKey="Ulrich R" first="Robert" last="Ulrich">Robert Ulrich</name>
<affiliation><mods:affiliation>Veterinary Research Institute, 62132 Brno, Czech Republic</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Gelnar, Milan" sort="Gelnar, Milan" uniqKey="Gelnar M" first="Milan" last="Gelnar">Milan Gelnar</name>
<affiliation><mods:affiliation>Masaryk University, Faculty of Science 61137 Brno Czech Republic</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Studnickova, Zdena" sort="Studnickova, Zdena" uniqKey="Studnickova Z" first="Zdena" last="Studnickova">Zdena Studnickova</name>
<affiliation><mods:affiliation>Masaryk University, Faculty of Science 61137 Brno Czech Republic</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Holoubek, Ivan" sort="Holoubek, Ivan" uniqKey="Holoubek I" first="Ivan" last="Holoubek">Ivan Holoubek</name>
<affiliation><mods:affiliation>Masaryk University, Faculty of Science 61137 Brno Czech Republic</mods:affiliation>
</affiliation>
<affiliation><mods:affiliation>RECETOX‐TOCOEN, 61137 Brno, Czech Republic</mods:affiliation>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">Environmental Toxicology and Chemistry</title>
<title level="j" type="sub">An International Journal</title>
<title level="j" type="abbrev">Environmental Toxicology and Chemistry</title>
<idno type="ISSN">0730-7268</idno>
<idno type="eISSN">1552-8618</idno>
<imprint><publisher>Wiley Periodicals, Inc.</publisher>
<pubPlace>Hoboken</pubPlace>
<date type="published" when="2001-05">2001-05</date>
<biblScope unit="volume">20</biblScope>
<biblScope unit="issue">5</biblScope>
<biblScope unit="page" from="1141">1141</biblScope>
<biblScope unit="page" to="1148">1148</biblScope>
</imprint>
<idno type="ISSN">0730-7268</idno>
</series>
<idno type="istex">3AE8F545906E07F2F216543FDEE8B60B8939F4E7</idno>
<idno type="DOI">10.1002/etc.5620200528</idno>
<idno type="ArticleID">ETC5620200528</idno>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0730-7268</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biomarker</term>
<term>CYP1A</term>
<term>Glutathione enzymes</term>
<term>Oxidative stress</term>
<term>Testosterone</term>
</keywords>
</textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Modulations of 11 prospective biochemical markers of impacts of aquatic pollutants in liver tissue of chub (Leuciscus cephalus), caught at several sampling sites of a river with various pollution types and rates, were matched against analytical data of concentrations of organochlorine compounds, polycyclic aromatic hydrocarbons (PAHs), and heavy metals. Multivariate principal component analysis (PCA) of the field data showed general patterns of biochemical responses to different types of pollutants and relationships among the biomarkers. Cytochrome P4501A‐dependent 7‐ethoxyresorufin O‐deethylase (EROD) activity, inducible by 2,3,7,8‐tetrachlorodibenzo‐p‐dioxin and structurally related planar compounds, was strongly enhanced in the more contaminated areas. Compared with polychlorinated aromatic hydrocarbons, PAHs did not contribute so significantly to EROD induction. Testosterone 6β‐ and 16α‐hydroxylase activities, as an expression of the cytochrome P4503A27, were slightly increased at several sites but were significantly decreased in samples from some heavily polluted areas. Recently, these activities have been suggested as potential biomarkers of exposure to contaminants that do not induce cytochrome P4501A. In this study, their inhibition or induction was not associated with a specific class of monitored contaminants, and selectivities of these modulations are still to be investigated. Similar modulations of the prospective biochemical indicators of oxidative stress, including microsomal glutathione S‐transferase activity, cytosolic glutathione S‐transferase with ethacrynic acid, and glutathione reductase, were demonstrated by PCA. The pattern of the modulations of the microsomal nicotinamide adenine dinucleotide phosphate (NADPH)‐dependent lipid peroxidation in vitro differed from the responses of the rest of oxidative stress parameters at some sampling sites. Further biochemical markers of oxidative stress under study, including in vivo lipid peroxidation, in vitro production of reactive oxygen species, and the concentration of metallothioneins did not correlate well with the concentrations of the contaminants. Principal component analysis demonstrated that the EROD activity, glutathione‐dependent enzymes, and Fe(II)‐enhanced lipid peroxidation formed a suitable battery of biomarkers of exposure.</div>
</front>
</TEI>
<istex><corpusName>wiley</corpusName>
<author><json:item><name>Miroslav Machala</name>
<affiliations><json:string>Veterinary Research Institute, 62132 Brno, Czech Republic</json:string>
<json:string>Veterinary Research Institute, 62132 Brno, Czech Republic</json:string>
</affiliations>
</json:item>
<json:item><name>Ladislav Dusek</name>
<affiliations><json:string>Masaryk University, Faculty of Science 61137 Brno Czech Republic</json:string>
<json:string>RECETOX‐TOCOEN, 61137 Brno, Czech Republic</json:string>
</affiliations>
</json:item>
<json:item><name>Klara Hilscherova</name>
<affiliations><json:string>Masaryk University, Faculty of Science 61137 Brno Czech Republic</json:string>
<json:string>RECETOX‐TOCOEN, 61137 Brno, Czech Republic</json:string>
</affiliations>
</json:item>
<json:item><name>Renata Kubinova</name>
<affiliations><json:string>Veterinary and Pharmaceutical University, 61200 Brno, Czech Republic</json:string>
</affiliations>
</json:item>
<json:item><name>Pavel Jurajda</name>
<affiliations><json:string>Landscape Ecology Institute, Academy of Sciences of the Czech Republic, Brno, Czech Republic</json:string>
</affiliations>
</json:item>
<json:item><name>Jiri Neca</name>
<affiliations><json:string>Veterinary Research Institute, 62132 Brno, Czech Republic</json:string>
</affiliations>
</json:item>
<json:item><name>Robert Ulrich</name>
<affiliations><json:string>Veterinary Research Institute, 62132 Brno, Czech Republic</json:string>
</affiliations>
</json:item>
<json:item><name>Milan Gelnar</name>
<affiliations><json:string>Masaryk University, Faculty of Science 61137 Brno Czech Republic</json:string>
</affiliations>
</json:item>
<json:item><name>Zdena Studnickova</name>
<affiliations><json:string>Masaryk University, Faculty of Science 61137 Brno Czech Republic</json:string>
</affiliations>
</json:item>
<json:item><name>Ivan Holoubek</name>
<affiliations><json:string>Masaryk University, Faculty of Science 61137 Brno Czech Republic</json:string>
<json:string>RECETOX‐TOCOEN, 61137 Brno, Czech Republic</json:string>
</affiliations>
</json:item>
</author>
<subject><json:item><lang><json:string>eng</json:string>
</lang>
<value>CYP1A</value>
</json:item>
<json:item><lang><json:string>eng</json:string>
</lang>
<value>Testosterone</value>
</json:item>
<json:item><lang><json:string>eng</json:string>
</lang>
<value>Glutathione enzymes</value>
</json:item>
<json:item><lang><json:string>eng</json:string>
</lang>
<value>Oxidative stress</value>
</json:item>
<json:item><lang><json:string>eng</json:string>
</lang>
<value>Biomarker</value>
</json:item>
</subject>
<articleId><json:string>ETC5620200528</json:string>
</articleId>
<language><json:string>eng</json:string>
</language>
<originalGenre><json:string>article</json:string>
</originalGenre>
<abstract>Modulations of 11 prospective biochemical markers of impacts of aquatic pollutants in liver tissue of chub (Leuciscus cephalus), caught at several sampling sites of a river with various pollution types and rates, were matched against analytical data of concentrations of organochlorine compounds, polycyclic aromatic hydrocarbons (PAHs), and heavy metals. Multivariate principal component analysis (PCA) of the field data showed general patterns of biochemical responses to different types of pollutants and relationships among the biomarkers. Cytochrome P4501A‐dependent 7‐ethoxyresorufin O‐deethylase (EROD) activity, inducible by 2,3,7,8‐tetrachlorodibenzo‐p‐dioxin and structurally related planar compounds, was strongly enhanced in the more contaminated areas. Compared with polychlorinated aromatic hydrocarbons, PAHs did not contribute so significantly to EROD induction. Testosterone 6β‐ and 16α‐hydroxylase activities, as an expression of the cytochrome P4503A27, were slightly increased at several sites but were significantly decreased in samples from some heavily polluted areas. Recently, these activities have been suggested as potential biomarkers of exposure to contaminants that do not induce cytochrome P4501A. In this study, their inhibition or induction was not associated with a specific class of monitored contaminants, and selectivities of these modulations are still to be investigated. Similar modulations of the prospective biochemical indicators of oxidative stress, including microsomal glutathione S‐transferase activity, cytosolic glutathione S‐transferase with ethacrynic acid, and glutathione reductase, were demonstrated by PCA. The pattern of the modulations of the microsomal nicotinamide adenine dinucleotide phosphate (NADPH)‐dependent lipid peroxidation in vitro differed from the responses of the rest of oxidative stress parameters at some sampling sites. Further biochemical markers of oxidative stress under study, including in vivo lipid peroxidation, in vitro production of reactive oxygen species, and the concentration of metallothioneins did not correlate well with the concentrations of the contaminants. Principal component analysis demonstrated that the EROD activity, glutathione‐dependent enzymes, and Fe(II)‐enhanced lipid peroxidation formed a suitable battery of biomarkers of exposure.</abstract>
<qualityIndicators><score>8</score>
<pdfVersion>1.3</pdfVersion>
<pdfPageSize>612 x 792 pts (letter)</pdfPageSize>
<refBibsNative>true</refBibsNative>
<abstractCharCount>2341</abstractCharCount>
<pdfWordCount>5521</pdfWordCount>
<pdfCharCount>37345</pdfCharCount>
<pdfPageCount>8</pdfPageCount>
<abstractWordCount>302</abstractWordCount>
</qualityIndicators>
<title>Determination and multivariate statistical analysis of biochemical responses to environmental contaminants in feral freshwater fish Leuciscus cephalus L.</title>
<genre><json:string>article</json:string>
</genre>
<host><volume>20</volume>
<publisherId><json:string>ETC</json:string>
</publisherId>
<pages><total>8</total>
<last>1148</last>
<first>1141</first>
</pages>
<issn><json:string>0730-7268</json:string>
</issn>
<issue>5</issue>
<subject><json:item><value>Hazard/Risk Assessment</value>
</json:item>
</subject>
<genre><json:string>journal</json:string>
</genre>
<language><json:string>unknown</json:string>
</language>
<eissn><json:string>1552-8618</json:string>
</eissn>
<title>Environmental Toxicology and Chemistry</title>
<doi><json:string>10.1002/(ISSN)1552-8618</json:string>
</doi>
</host>
<categories><wos><json:string>science</json:string>
<json:string>toxicology</json:string>
<json:string>environmental sciences</json:string>
</wos>
<scienceMetrix><json:string>natural sciences</json:string>
<json:string>earth & environmental sciences</json:string>
<json:string>environmental sciences</json:string>
</scienceMetrix>
</categories>
<publicationDate>2001</publicationDate>
<copyrightDate>2001</copyrightDate>
<doi><json:string>10.1002/etc.5620200528</json:string>
</doi>
<id>3AE8F545906E07F2F216543FDEE8B60B8939F4E7</id>
<score>0.020024013</score>
<fulltext><json:item><extension>pdf</extension>
<original>true</original>
<mimetype>application/pdf</mimetype>
<uri>https://api.istex.fr/document/3AE8F545906E07F2F216543FDEE8B60B8939F4E7/fulltext/pdf</uri>
</json:item>
<json:item><extension>zip</extension>
<original>false</original>
<mimetype>application/zip</mimetype>
<uri>https://api.istex.fr/document/3AE8F545906E07F2F216543FDEE8B60B8939F4E7/fulltext/zip</uri>
</json:item>
<istex:fulltextTEI uri="https://api.istex.fr/document/3AE8F545906E07F2F216543FDEE8B60B8939F4E7/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Determination and multivariate statistical analysis of biochemical responses to environmental contaminants in feral freshwater fish Leuciscus cephalus L.</title>
</titleStmt>
<publicationStmt><authority>ISTEX</authority>
<publisher>Wiley Periodicals, Inc.</publisher>
<pubPlace>Hoboken</pubPlace>
<availability><p>Copyright © 2001 SETAC</p>
</availability>
<date>2001</date>
</publicationStmt>
<sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Determination and multivariate statistical analysis of biochemical responses to environmental contaminants in feral freshwater fish Leuciscus cephalus L.</title>
<author xml:id="author-1"><persName><forename type="first">Miroslav</forename>
<surname>Machala</surname>
</persName>
<affiliation>Veterinary Research Institute, 62132 Brno, Czech Republic</affiliation>
<affiliation>Veterinary Research Institute, 62132 Brno, Czech Republic</affiliation>
</author>
<author xml:id="author-2"><persName><forename type="first">Ladislav</forename>
<surname>Dusek</surname>
</persName>
<affiliation>Masaryk University, Faculty of Science 61137 Brno Czech Republic</affiliation>
<affiliation>RECETOX‐TOCOEN, 61137 Brno, Czech Republic</affiliation>
</author>
<author xml:id="author-3"><persName><forename type="first">Klara</forename>
<surname>Hilscherova</surname>
</persName>
<affiliation>Masaryk University, Faculty of Science 61137 Brno Czech Republic</affiliation>
<affiliation>RECETOX‐TOCOEN, 61137 Brno, Czech Republic</affiliation>
</author>
<author xml:id="author-4"><persName><forename type="first">Renata</forename>
<surname>Kubinova</surname>
</persName>
<affiliation>Veterinary and Pharmaceutical University, 61200 Brno, Czech Republic</affiliation>
</author>
<author xml:id="author-5"><persName><forename type="first">Pavel</forename>
<surname>Jurajda</surname>
</persName>
<affiliation>Landscape Ecology Institute, Academy of Sciences of the Czech Republic, Brno, Czech Republic</affiliation>
</author>
<author xml:id="author-6"><persName><forename type="first">Jiri</forename>
<surname>Neca</surname>
</persName>
<affiliation>Veterinary Research Institute, 62132 Brno, Czech Republic</affiliation>
</author>
<author xml:id="author-7"><persName><forename type="first">Robert</forename>
<surname>Ulrich</surname>
</persName>
<affiliation>Veterinary Research Institute, 62132 Brno, Czech Republic</affiliation>
</author>
<author xml:id="author-8"><persName><forename type="first">Milan</forename>
<surname>Gelnar</surname>
</persName>
<affiliation>Masaryk University, Faculty of Science 61137 Brno Czech Republic</affiliation>
</author>
<author xml:id="author-9"><persName><forename type="first">Zdena</forename>
<surname>Studnickova</surname>
</persName>
<affiliation>Masaryk University, Faculty of Science 61137 Brno Czech Republic</affiliation>
</author>
<author xml:id="author-10"><persName><forename type="first">Ivan</forename>
<surname>Holoubek</surname>
</persName>
<affiliation>Masaryk University, Faculty of Science 61137 Brno Czech Republic</affiliation>
<affiliation>RECETOX‐TOCOEN, 61137 Brno, Czech Republic</affiliation>
</author>
</analytic>
<monogr><title level="j">Environmental Toxicology and Chemistry</title>
<title level="j" type="sub">An International Journal</title>
<title level="j" type="abbrev">Environmental Toxicology and Chemistry</title>
<idno type="pISSN">0730-7268</idno>
<idno type="eISSN">1552-8618</idno>
<idno type="DOI">10.1002/(ISSN)1552-8618</idno>
<imprint><publisher>Wiley Periodicals, Inc.</publisher>
<pubPlace>Hoboken</pubPlace>
<date type="published" when="2001-05"></date>
<biblScope unit="volume">20</biblScope>
<biblScope unit="issue">5</biblScope>
<biblScope unit="page" from="1141">1141</biblScope>
<biblScope unit="page" to="1148">1148</biblScope>
</imprint>
</monogr>
<idno type="istex">3AE8F545906E07F2F216543FDEE8B60B8939F4E7</idno>
<idno type="DOI">10.1002/etc.5620200528</idno>
<idno type="ArticleID">ETC5620200528</idno>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><creation><date>2001</date>
</creation>
<langUsage><language ident="en">en</language>
</langUsage>
<abstract xml:lang="en"><p>Modulations of 11 prospective biochemical markers of impacts of aquatic pollutants in liver tissue of chub (Leuciscus cephalus), caught at several sampling sites of a river with various pollution types and rates, were matched against analytical data of concentrations of organochlorine compounds, polycyclic aromatic hydrocarbons (PAHs), and heavy metals. Multivariate principal component analysis (PCA) of the field data showed general patterns of biochemical responses to different types of pollutants and relationships among the biomarkers. Cytochrome P4501A‐dependent 7‐ethoxyresorufin O‐deethylase (EROD) activity, inducible by 2,3,7,8‐tetrachlorodibenzo‐p‐dioxin and structurally related planar compounds, was strongly enhanced in the more contaminated areas. Compared with polychlorinated aromatic hydrocarbons, PAHs did not contribute so significantly to EROD induction. Testosterone 6β‐ and 16α‐hydroxylase activities, as an expression of the cytochrome P4503A27, were slightly increased at several sites but were significantly decreased in samples from some heavily polluted areas. Recently, these activities have been suggested as potential biomarkers of exposure to contaminants that do not induce cytochrome P4501A. In this study, their inhibition or induction was not associated with a specific class of monitored contaminants, and selectivities of these modulations are still to be investigated. Similar modulations of the prospective biochemical indicators of oxidative stress, including microsomal glutathione S‐transferase activity, cytosolic glutathione S‐transferase with ethacrynic acid, and glutathione reductase, were demonstrated by PCA. The pattern of the modulations of the microsomal nicotinamide adenine dinucleotide phosphate (NADPH)‐dependent lipid peroxidation in vitro differed from the responses of the rest of oxidative stress parameters at some sampling sites. Further biochemical markers of oxidative stress under study, including in vivo lipid peroxidation, in vitro production of reactive oxygen species, and the concentration of metallothioneins did not correlate well with the concentrations of the contaminants. Principal component analysis demonstrated that the EROD activity, glutathione‐dependent enzymes, and Fe(II)‐enhanced lipid peroxidation formed a suitable battery of biomarkers of exposure.</p>
</abstract>
<textClass xml:lang="en"><keywords scheme="keyword"><list><head>keywords</head>
<item><term>CYP1A</term>
</item>
<item><term>Testosterone</term>
</item>
<item><term>Glutathione enzymes</term>
</item>
<item><term>Oxidative stress</term>
</item>
<item><term>Biomarker</term>
</item>
</list>
</keywords>
</textClass>
<textClass><keywords scheme="Journal Subject"><list><head>article-category</head>
<item><term>Hazard/Risk Assessment</term>
</item>
</list>
</keywords>
</textClass>
</profileDesc>
<revisionDesc><change when="1999-12-01">Received</change>
<change when="2000-10-13">Registration</change>
<change when="2001-05">Published</change>
</revisionDesc>
</teiHeader>
</istex:fulltextTEI>
<json:item><extension>txt</extension>
<original>false</original>
<mimetype>text/plain</mimetype>
<uri>https://api.istex.fr/document/3AE8F545906E07F2F216543FDEE8B60B8939F4E7/fulltext/txt</uri>
</json:item>
</fulltext>
<metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration>
<istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>Wiley Periodicals, Inc.</publisherName>
<publisherLoc>Hoboken</publisherLoc>
</publisherInfo>
<doi registered="yes">10.1002/(ISSN)1552-8618</doi>
<issn type="print">0730-7268</issn>
<issn type="electronic">1552-8618</issn>
<idGroup><id type="product" value="ETC"></id>
</idGroup>
<titleGroup><title type="main" xml:lang="en" sort="ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY">Environmental Toxicology and Chemistry</title>
<title type="subtitle">An International Journal</title>
<title type="short">Environmental Toxicology and Chemistry</title>
</titleGroup>
</publicationMeta>
<publicationMeta level="part" position="50"><doi origin="wiley" registered="no">10.1002/etc.v20:5</doi>
<numberingGroup><numbering type="journalVolume" number="20">20</numbering>
<numbering type="journalIssue">5</numbering>
</numberingGroup>
<coverDate startDate="2001-05">May 2001</coverDate>
</publicationMeta>
<publicationMeta level="unit" type="article" position="28" status="forIssue"><doi origin="wiley" registered="no">10.1002/etc.5620200528</doi>
<idGroup><id type="unit" value="ETC5620200528"></id>
</idGroup>
<countGroup><count type="pageTotal" number="8"></count>
</countGroup>
<titleGroup><title type="articleCategory">Hazard/Risk Assessment</title>
<title type="tocHeading1">Hazard/Risk Assessments</title>
</titleGroup>
<copyright ownership="thirdParty">Copyright © 2001 SETAC</copyright>
<eventGroup><event type="manuscriptReceived" date="1999-12-01"></event>
<event type="manuscriptAccepted" date="2000-10-13"></event>
<event type="firstOnline" date="2009-11-03"></event>
<event type="publishedOnlineFinalForm" date="2009-11-03"></event>
<event type="xmlConverted" agent="Converter:JWSART34_TO_WML3G version:2.3.5 mode:FullText source:FullText result:FullText" date="2010-04-09"></event>
<event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:3.8.8" date="2014-01-25"></event>
<event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-10-16"></event>
</eventGroup>
<numberingGroup><numbering type="pageFirst">1141</numbering>
<numbering type="pageLast">1148</numbering>
</numberingGroup>
<correspondenceTo>Veterinary Research Institute, 62132 Brno, Czech Republic</correspondenceTo>
<linkGroup><link type="toTypesetVersion" href="file:ETC.ETC5620200528.pdf"></link>
</linkGroup>
</publicationMeta>
<contentMeta><countGroup><count type="figureTotal" number="6"></count>
<count type="tableTotal" number="1"></count>
<count type="referenceTotal" number="44"></count>
</countGroup>
<titleGroup><title type="main" xml:lang="en">Determination and multivariate statistical analysis of biochemical responses to environmental contaminants in feral freshwater fish <i>Leuciscus cephalus</i>
L.</title>
<title type="short" xml:lang="en">Biomarkers of contamination in feral fish</title>
</titleGroup>
<creators><creator xml:id="au1" creatorRole="author" affiliationRef="#af1" corresponding="yes"><personName><givenNames>Miroslav</givenNames>
<familyName>Machala</familyName>
</personName>
<contactDetails><email>machala@vri.cz</email>
</contactDetails>
</creator>
<creator xml:id="au2" creatorRole="author" affiliationRef="#af2 #af3"><personName><givenNames>Ladislav</givenNames>
<familyName>Dusek</familyName>
</personName>
</creator>
<creator xml:id="au3" creatorRole="author" affiliationRef="#af2 #af3"><personName><givenNames>Klara</givenNames>
<familyName>Hilscherova</familyName>
</personName>
</creator>
<creator xml:id="au4" creatorRole="author" affiliationRef="#af4"><personName><givenNames>Renata</givenNames>
<familyName>Kubinova</familyName>
</personName>
</creator>
<creator xml:id="au5" creatorRole="author" affiliationRef="#af5"><personName><givenNames>Pavel</givenNames>
<familyName>Jurajda</familyName>
</personName>
</creator>
<creator xml:id="au6" creatorRole="author" affiliationRef="#af1"><personName><givenNames>Jiri</givenNames>
<familyName>Neca</familyName>
</personName>
</creator>
<creator xml:id="au7" creatorRole="author" affiliationRef="#af1"><personName><givenNames>Robert</givenNames>
<familyName>Ulrich</familyName>
</personName>
</creator>
<creator xml:id="au8" creatorRole="author" affiliationRef="#af2"><personName><givenNames>Milan</givenNames>
<familyName>Gelnar</familyName>
</personName>
</creator>
<creator xml:id="au9" creatorRole="author" affiliationRef="#af2"><personName><givenNames>Zdena</givenNames>
<familyName>Studnickova</familyName>
</personName>
</creator>
<creator xml:id="au10" creatorRole="author" affiliationRef="#af2 #af3"><personName><givenNames>Ivan</givenNames>
<familyName>Holoubek</familyName>
</personName>
</creator>
</creators>
<affiliationGroup><affiliation xml:id="af1" countryCode="CZ" type="organization"><unparsedAffiliation>Veterinary Research Institute, 62132 Brno, Czech Republic</unparsedAffiliation>
</affiliation>
<affiliation xml:id="af2" countryCode="CZ" type="organization"><unparsedAffiliation>Masaryk University, Faculty of Science 61137 Brno Czech Republic</unparsedAffiliation>
</affiliation>
<affiliation xml:id="af3" countryCode="CZ" type="organization"><unparsedAffiliation>RECETOX‐TOCOEN, 61137 Brno, Czech Republic</unparsedAffiliation>
</affiliation>
<affiliation xml:id="af4" countryCode="CZ" type="organization"><unparsedAffiliation>Veterinary and Pharmaceutical University, 61200 Brno, Czech Republic</unparsedAffiliation>
</affiliation>
<affiliation xml:id="af5" countryCode="CZ" type="organization"><unparsedAffiliation>Landscape Ecology Institute, Academy of Sciences of the Czech Republic, Brno, Czech Republic</unparsedAffiliation>
</affiliation>
</affiliationGroup>
<keywordGroup xml:lang="en" type="author"><keyword xml:id="kwd1">CYP1A</keyword>
<keyword xml:id="kwd2">Testosterone</keyword>
<keyword xml:id="kwd3">Glutathione enzymes</keyword>
<keyword xml:id="kwd4">Oxidative stress</keyword>
<keyword xml:id="kwd5">Biomarker</keyword>
</keywordGroup>
<abstractGroup><abstract type="main" xml:lang="en"><title type="main">Abstract</title>
<p>Modulations of 11 prospective biochemical markers of impacts of aquatic pollutants in liver tissue of chub (<i>Leuciscus cephalus</i>
), caught at several sampling sites of a river with various pollution types and rates, were matched against analytical data of concentrations of organochlorine compounds, polycyclic aromatic hydrocarbons (PAHs), and heavy metals. Multivariate principal component analysis (PCA) of the field data showed general patterns of biochemical responses to different types of pollutants and relationships among the biomarkers. Cytochrome P4501A‐dependent 7‐ethoxyresorufin <i>O</i>
‐deethylase (EROD) activity, inducible by 2,3,7,8‐tetrachlorodibenzo‐<i>p</i>
‐dioxin and structurally related planar compounds, was strongly enhanced in the more contaminated areas. Compared with polychlorinated aromatic hydrocarbons, PAHs did not contribute so significantly to EROD induction. Testosterone 6β‐ and 16α‐hydroxylase activities, as an expression of the cytochrome P4503A27, were slightly increased at several sites but were significantly decreased in samples from some heavily polluted areas. Recently, these activities have been suggested as potential biomarkers of exposure to contaminants that do not induce cytochrome P4501A. In this study, their inhibition or induction was not associated with a specific class of monitored contaminants, and selectivities of these modulations are still to be investigated. Similar modulations of the prospective biochemical indicators of oxidative stress, including microsomal glutathione S‐transferase activity, cytosolic glutathione <i>S</i>
‐transferase with ethacrynic acid, and glutathione reductase, were demonstrated by PCA. The pattern of the modulations of the microsomal nicotinamide adenine dinucleotide phosphate (NADPH)‐dependent lipid peroxidation in vitro differed from the responses of the rest of oxidative stress parameters at some sampling sites. Further biochemical markers of oxidative stress under study, including in vivo lipid peroxidation, in vitro production of reactive oxygen species, and the concentration of metallothioneins did not correlate well with the concentrations of the contaminants. Principal component analysis demonstrated that the EROD activity, glutathione‐dependent enzymes, and Fe(II)‐enhanced lipid peroxidation formed a suitable battery of biomarkers of exposure.</p>
</abstract>
</abstractGroup>
</contentMeta>
</header>
</component>
</istex:document>
</istex:metadataXml>
<mods version="3.6"><titleInfo lang="en"><title>Determination and multivariate statistical analysis of biochemical responses to environmental contaminants in feral freshwater fish Leuciscus cephalus L.</title>
</titleInfo>
<titleInfo type="abbreviated" lang="en"><title>Biomarkers of contamination in feral fish</title>
</titleInfo>
<titleInfo type="alternative" contentType="CDATA" lang="en"><title>Determination and multivariate statistical analysis of biochemical responses to environmental contaminants in feral freshwater fish Leuciscus cephalus L.</title>
</titleInfo>
<name type="personal"><namePart type="given">Miroslav</namePart>
<namePart type="family">Machala</namePart>
<affiliation>Veterinary Research Institute, 62132 Brno, Czech Republic</affiliation>
<affiliation>Veterinary Research Institute, 62132 Brno, Czech Republic</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">Ladislav</namePart>
<namePart type="family">Dusek</namePart>
<affiliation>Masaryk University, Faculty of Science 61137 Brno Czech Republic</affiliation>
<affiliation>RECETOX‐TOCOEN, 61137 Brno, Czech Republic</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">Klara</namePart>
<namePart type="family">Hilscherova</namePart>
<affiliation>Masaryk University, Faculty of Science 61137 Brno Czech Republic</affiliation>
<affiliation>RECETOX‐TOCOEN, 61137 Brno, Czech Republic</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">Renata</namePart>
<namePart type="family">Kubinova</namePart>
<affiliation>Veterinary and Pharmaceutical University, 61200 Brno, Czech Republic</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">Pavel</namePart>
<namePart type="family">Jurajda</namePart>
<affiliation>Landscape Ecology Institute, Academy of Sciences of the Czech Republic, Brno, Czech Republic</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">Jiri</namePart>
<namePart type="family">Neca</namePart>
<affiliation>Veterinary Research Institute, 62132 Brno, Czech Republic</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">Robert</namePart>
<namePart type="family">Ulrich</namePart>
<affiliation>Veterinary Research Institute, 62132 Brno, Czech Republic</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">Milan</namePart>
<namePart type="family">Gelnar</namePart>
<affiliation>Masaryk University, Faculty of Science 61137 Brno Czech Republic</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">Zdena</namePart>
<namePart type="family">Studnickova</namePart>
<affiliation>Masaryk University, Faculty of Science 61137 Brno Czech Republic</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">Ivan</namePart>
<namePart type="family">Holoubek</namePart>
<affiliation>Masaryk University, Faculty of Science 61137 Brno Czech Republic</affiliation>
<affiliation>RECETOX‐TOCOEN, 61137 Brno, Czech Republic</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<typeOfResource>text</typeOfResource>
<genre type="article" displayLabel="article"></genre>
<originInfo><publisher>Wiley Periodicals, Inc.</publisher>
<place><placeTerm type="text">Hoboken</placeTerm>
</place>
<dateIssued encoding="w3cdtf">2001-05</dateIssued>
<dateCaptured encoding="w3cdtf">1999-12-01</dateCaptured>
<dateValid encoding="w3cdtf">2000-10-13</dateValid>
<copyrightDate encoding="w3cdtf">2001</copyrightDate>
</originInfo>
<language><languageTerm type="code" authority="rfc3066">en</languageTerm>
<languageTerm type="code" authority="iso639-2b">eng</languageTerm>
</language>
<physicalDescription><internetMediaType>text/html</internetMediaType>
<extent unit="figures">6</extent>
<extent unit="tables">1</extent>
<extent unit="references">44</extent>
</physicalDescription>
<abstract lang="en">Modulations of 11 prospective biochemical markers of impacts of aquatic pollutants in liver tissue of chub (Leuciscus cephalus), caught at several sampling sites of a river with various pollution types and rates, were matched against analytical data of concentrations of organochlorine compounds, polycyclic aromatic hydrocarbons (PAHs), and heavy metals. Multivariate principal component analysis (PCA) of the field data showed general patterns of biochemical responses to different types of pollutants and relationships among the biomarkers. Cytochrome P4501A‐dependent 7‐ethoxyresorufin O‐deethylase (EROD) activity, inducible by 2,3,7,8‐tetrachlorodibenzo‐p‐dioxin and structurally related planar compounds, was strongly enhanced in the more contaminated areas. Compared with polychlorinated aromatic hydrocarbons, PAHs did not contribute so significantly to EROD induction. Testosterone 6β‐ and 16α‐hydroxylase activities, as an expression of the cytochrome P4503A27, were slightly increased at several sites but were significantly decreased in samples from some heavily polluted areas. Recently, these activities have been suggested as potential biomarkers of exposure to contaminants that do not induce cytochrome P4501A. In this study, their inhibition or induction was not associated with a specific class of monitored contaminants, and selectivities of these modulations are still to be investigated. Similar modulations of the prospective biochemical indicators of oxidative stress, including microsomal glutathione S‐transferase activity, cytosolic glutathione S‐transferase with ethacrynic acid, and glutathione reductase, were demonstrated by PCA. The pattern of the modulations of the microsomal nicotinamide adenine dinucleotide phosphate (NADPH)‐dependent lipid peroxidation in vitro differed from the responses of the rest of oxidative stress parameters at some sampling sites. Further biochemical markers of oxidative stress under study, including in vivo lipid peroxidation, in vitro production of reactive oxygen species, and the concentration of metallothioneins did not correlate well with the concentrations of the contaminants. Principal component analysis demonstrated that the EROD activity, glutathione‐dependent enzymes, and Fe(II)‐enhanced lipid peroxidation formed a suitable battery of biomarkers of exposure.</abstract>
<subject lang="en"><genre>keywords</genre>
<topic>CYP1A</topic>
<topic>Testosterone</topic>
<topic>Glutathione enzymes</topic>
<topic>Oxidative stress</topic>
<topic>Biomarker</topic>
</subject>
<relatedItem type="host"><titleInfo><title>Environmental Toxicology and Chemistry</title>
<subTitle>An International Journal</subTitle>
</titleInfo>
<titleInfo type="abbreviated"><title>Environmental Toxicology and Chemistry</title>
</titleInfo>
<genre type="journal">journal</genre>
<subject><genre>article-category</genre>
<topic>Hazard/Risk Assessment</topic>
</subject>
<identifier type="ISSN">0730-7268</identifier>
<identifier type="eISSN">1552-8618</identifier>
<identifier type="DOI">10.1002/(ISSN)1552-8618</identifier>
<identifier type="PublisherID">ETC</identifier>
<part><date>2001</date>
<detail type="volume"><caption>vol.</caption>
<number>20</number>
</detail>
<detail type="issue"><caption>no.</caption>
<number>5</number>
</detail>
<extent unit="pages"><start>1141</start>
<end>1148</end>
<total>8</total>
</extent>
</part>
</relatedItem>
<identifier type="istex">3AE8F545906E07F2F216543FDEE8B60B8939F4E7</identifier>
<identifier type="DOI">10.1002/etc.5620200528</identifier>
<identifier type="ArticleID">ETC5620200528</identifier>
<accessCondition type="use and reproduction" contentType="copyright">Copyright © 2001 SETAC</accessCondition>
<recordInfo><recordContentSource>WILEY</recordContentSource>
<recordOrigin>Wiley Periodicals, Inc.</recordOrigin>
</recordInfo>
</mods>
</metadata>
<serie></serie>
</istex>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Eau/explor/EsturgeonV1/Data/Istex/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001404 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Corpus/biblio.hfd -nk 001404 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Eau |area= EsturgeonV1 |flux= Istex |étape= Corpus |type= RBID |clé= ISTEX:3AE8F545906E07F2F216543FDEE8B60B8939F4E7 |texte= Determination and multivariate statistical analysis of biochemical responses to environmental contaminants in feral freshwater fish Leuciscus cephalus L. }}
![]() | This area was generated with Dilib version V0.6.27. | ![]() |