An evaluation of the etiology of reduced CYP1A1 messenger RNA expression in the Atlantic tomcod from the Hudson River, New York, USA, using reverse transcriptase polymerase chain reaction analysis
Identifieur interne : 001402 ( Istex/Corpus ); précédent : 001401; suivant : 001403An evaluation of the etiology of reduced CYP1A1 messenger RNA expression in the Atlantic tomcod from the Hudson River, New York, USA, using reverse transcriptase polymerase chain reaction analysis
Auteurs : Nirmal K. Roy ; Simon Courtenay ; Zhanpeng Yuan ; Michael Ikonomou ; Isaac WirginSource :
- Environmental Toxicology and Chemistry [ 0730-7268 ] ; 2001-05.
English descriptors
Abstract
Adult Atlantic tomcod, Microgadus tomcod, from the Hudson River, New York State, USA, exhibit reduced inducibility of hepatic cytochrome P4501A1 (CYP1A1) mRNA compared with adult tomcod from the cleaner Miramichi River, New Brunswick, Canada, when treated with coplanar polychlorinated biphenyl (PCB) congeners or 2,3,7,8‐tetrachlorodibenzo‐p‐dioxin. In contrast, little difference in CYP1A1 inducibility is observed between tomcod from these two rivers when treated with polycyclic aromatic hydrocarbons (PAHs). We sought to determine if impaired hepatic CYP1A1 inducibility in Hudson River tomcod results from a multigenerational, genetic adaptation or a single generational, physiological acclimation. Embryos and larvae from controlled experimental crosses of Hudson River and Miramichi River parents were exposed for 24 h to water‐borne PCB congener 77 (10 ppm), benzo[a]pyrene (BaP; 10 ppm), or dimethysulfoxide, and CYP1A1 expression was assessed in individual larva using competitive reverse transcriptase polymerase chain reaction (RT‐PCR) analysis. The CYP1A1 mRNA was significantly induced in larvae from both populations by BaP (47‐ and 52‐fold) and PCB 77 (9‐ and 22‐fold), although levels of expression were higher in offspring of Miramichi matings. Most important, CYP1A1 mRNA was significantly induced by PCB 77 in larvae from Hudson River parents. Concentrations of dioxin, furan, and PCB congeners were measured in livers and eggs of female tomcod from these two locales to quantify the extent of maternal transfer of contaminants. For both rivers, wet‐weight contaminant concentrations were significantly higher (4–7 times) in livers than in eggs of the same females, suggesting that a threshold level of contaminants may have to be reached before CYP1A1 transcription is impaired. We conclude that reduced inducibility of hepatic CYP1A1 mRNA in adult tomcod from the Hudson River is most consistent with single‐generational acclimation.
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DOI: 10.1002/etc.5620200512
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Roy</name><affiliation><mods:affiliation>Department of Environmental Medicine, New York University School of Medicine, 57 Old Forge Road, Tuxedo, New York 10987, USA</mods:affiliation></affiliation></author><author><name sortKey="Courtenay, Simon" sort="Courtenay, Simon" uniqKey="Courtenay S" first="Simon" last="Courtenay">Simon Courtenay</name><affiliation><mods:affiliation>Fisheries and Oceans Canada, Gulf Fisheries Centre, PO Box 5030, Moncton, New Brunswick E1C 9B6, Canada</mods:affiliation></affiliation></author><author><name sortKey="Yuan, Zhanpeng" sort="Yuan, Zhanpeng" uniqKey="Yuan Z" first="Zhanpeng" last="Yuan">Zhanpeng Yuan</name><affiliation><mods:affiliation>Department of Environmental Medicine, New York University School of Medicine, 57 Old Forge Road, Tuxedo, New York 10987, USA</mods:affiliation></affiliation></author><author><name sortKey="Ikonomou, Michael" sort="Ikonomou, Michael" uniqKey="Ikonomou M" first="Michael" last="Ikonomou">Michael Ikonomou</name><affiliation><mods:affiliation>Fisheries and Oceans Canada, Institute of Ocean Science, PO Box 9860, Sidney, British Columbia V8L 4B2, Canada</mods:affiliation></affiliation></author><author><name sortKey="Wirgin, Isaac" sort="Wirgin, Isaac" uniqKey="Wirgin I" first="Isaac" last="Wirgin">Isaac Wirgin</name><affiliation><mods:affiliation>Department of Environmental Medicine, New York University School of Medicine, 57 Old Forge Road, Tuxedo, New York 10987, USA</mods:affiliation></affiliation><affiliation><mods:affiliation>Department of Environmental Medicine, New York University School of Medicine, 57 Old Forge Road, Tuxedo, New York 10987, USA</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Environmental Toxicology and Chemistry</title><title level="j" type="sub">An International Journal</title><title level="j" type="abbrev">Environmental Toxicology and Chemistry</title><idno type="ISSN">0730-7268</idno><idno type="eISSN">1552-8618</idno><imprint><publisher>Wiley Periodicals, Inc.</publisher><pubPlace>Hoboken</pubPlace><date type="published" when="2001-05">2001-05</date><biblScope unit="volume">20</biblScope><biblScope unit="issue">5</biblScope><biblScope unit="page" from="1022">1022</biblScope><biblScope unit="page" to="1030">1030</biblScope></imprint><idno type="ISSN">0730-7268</idno></series><idno type="istex">3539CE898630A1A56D588CE70846580FB7D553B8</idno><idno type="DOI">10.1002/etc.5620200512</idno><idno type="ArticleID">ETC5620200512</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0730-7268</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cytochrome P4501A1</term><term>Dioxins</term><term>Furans</term><term>Gene expression</term><term>Polychlorinated biphenyls</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Adult Atlantic tomcod, Microgadus tomcod, from the Hudson River, New York State, USA, exhibit reduced inducibility of hepatic cytochrome P4501A1 (CYP1A1) mRNA compared with adult tomcod from the cleaner Miramichi River, New Brunswick, Canada, when treated with coplanar polychlorinated biphenyl (PCB) congeners or 2,3,7,8‐tetrachlorodibenzo‐p‐dioxin. In contrast, little difference in CYP1A1 inducibility is observed between tomcod from these two rivers when treated with polycyclic aromatic hydrocarbons (PAHs). We sought to determine if impaired hepatic CYP1A1 inducibility in Hudson River tomcod results from a multigenerational, genetic adaptation or a single generational, physiological acclimation. Embryos and larvae from controlled experimental crosses of Hudson River and Miramichi River parents were exposed for 24 h to water‐borne PCB congener 77 (10 ppm), benzo[a]pyrene (BaP; 10 ppm), or dimethysulfoxide, and CYP1A1 expression was assessed in individual larva using competitive reverse transcriptase polymerase chain reaction (RT‐PCR) analysis. The CYP1A1 mRNA was significantly induced in larvae from both populations by BaP (47‐ and 52‐fold) and PCB 77 (9‐ and 22‐fold), although levels of expression were higher in offspring of Miramichi matings. Most important, CYP1A1 mRNA was significantly induced by PCB 77 in larvae from Hudson River parents. Concentrations of dioxin, furan, and PCB congeners were measured in livers and eggs of female tomcod from these two locales to quantify the extent of maternal transfer of contaminants. For both rivers, wet‐weight contaminant concentrations were significantly higher (4–7 times) in livers than in eggs of the same females, suggesting that a threshold level of contaminants may have to be reached before CYP1A1 transcription is impaired. We conclude that reduced inducibility of hepatic CYP1A1 mRNA in adult tomcod from the Hudson River is most consistent with single‐generational acclimation.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>Nirmal K. Roy</name><affiliations><json:string>Department of Environmental Medicine, New York University School of Medicine, 57 Old Forge Road, Tuxedo, New York 10987, USA</json:string></affiliations></json:item><json:item><name>Simon Courtenay</name><affiliations><json:string>Fisheries and Oceans Canada, Gulf Fisheries Centre, PO Box 5030, Moncton, New Brunswick E1C 9B6, Canada</json:string></affiliations></json:item><json:item><name>Zhanpeng Yuan</name><affiliations><json:string>Department of Environmental Medicine, New York University School of Medicine, 57 Old Forge Road, Tuxedo, New York 10987, USA</json:string></affiliations></json:item><json:item><name>Michael Ikonomou</name><affiliations><json:string>Fisheries and Oceans Canada, Institute of Ocean Science, PO Box 9860, Sidney, British Columbia V8L 4B2, Canada</json:string></affiliations></json:item><json:item><name>Isaac Wirgin</name><affiliations><json:string>Department of Environmental Medicine, New York University School of Medicine, 57 Old Forge Road, Tuxedo, New York 10987, USA</json:string><json:string>Department of Environmental Medicine, New York University School of Medicine, 57 Old Forge Road, Tuxedo, New York 10987, USA</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Cytochrome P4501A1</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Gene expression</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Polychlorinated biphenyls</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Dioxins</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Furans</value></json:item></subject><articleId><json:string>ETC5620200512</json:string></articleId><language><json:string>eng</json:string></language><originalGenre><json:string>article</json:string></originalGenre><abstract>Adult Atlantic tomcod, Microgadus tomcod, from the Hudson River, New York State, USA, exhibit reduced inducibility of hepatic cytochrome P4501A1 (CYP1A1) mRNA compared with adult tomcod from the cleaner Miramichi River, New Brunswick, Canada, when treated with coplanar polychlorinated biphenyl (PCB) congeners or 2,3,7,8‐tetrachlorodibenzo‐p‐dioxin. In contrast, little difference in CYP1A1 inducibility is observed between tomcod from these two rivers when treated with polycyclic aromatic hydrocarbons (PAHs). We sought to determine if impaired hepatic CYP1A1 inducibility in Hudson River tomcod results from a multigenerational, genetic adaptation or a single generational, physiological acclimation. Embryos and larvae from controlled experimental crosses of Hudson River and Miramichi River parents were exposed for 24 h to water‐borne PCB congener 77 (10 ppm), benzo[a]pyrene (BaP; 10 ppm), or dimethysulfoxide, and CYP1A1 expression was assessed in individual larva using competitive reverse transcriptase polymerase chain reaction (RT‐PCR) analysis. The CYP1A1 mRNA was significantly induced in larvae from both populations by BaP (47‐ and 52‐fold) and PCB 77 (9‐ and 22‐fold), although levels of expression were higher in offspring of Miramichi matings. Most important, CYP1A1 mRNA was significantly induced by PCB 77 in larvae from Hudson River parents. Concentrations of dioxin, furan, and PCB congeners were measured in livers and eggs of female tomcod from these two locales to quantify the extent of maternal transfer of contaminants. For both rivers, wet‐weight contaminant concentrations were significantly higher (4–7 times) in livers than in eggs of the same females, suggesting that a threshold level of contaminants may have to be reached before CYP1A1 transcription is impaired. 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In contrast, little difference in CYP1A1 inducibility is observed between tomcod from these two rivers when treated with polycyclic aromatic hydrocarbons (PAHs). We sought to determine if impaired hepatic CYP1A1 inducibility in Hudson River tomcod results from a multigenerational, genetic adaptation or a single generational, physiological acclimation. Embryos and larvae from controlled experimental crosses of Hudson River and Miramichi River parents were exposed for 24 h to water‐borne PCB congener 77 (10 ppm), benzo[a]pyrene (BaP; 10 ppm), or dimethysulfoxide, and CYP1A1 expression was assessed in individual larva using competitive reverse transcriptase polymerase chain reaction (RT‐PCR) analysis. The CYP1A1 mRNA was significantly induced in larvae from both populations by BaP (47‐ and 52‐fold) and PCB 77 (9‐ and 22‐fold), although levels of expression were higher in offspring of Miramichi matings. Most important, CYP1A1 mRNA was significantly induced by PCB 77 in larvae from Hudson River parents. Concentrations of dioxin, furan, and PCB congeners were measured in livers and eggs of female tomcod from these two locales to quantify the extent of maternal transfer of contaminants. For both rivers, wet‐weight contaminant concentrations were significantly higher (4–7 times) in livers than in eggs of the same females, suggesting that a threshold level of contaminants may have to be reached before CYP1A1 transcription is impaired. 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Canada</unparsedAffiliation></affiliation><affiliation xml:id="af3" countryCode="CA" type="organization"><unparsedAffiliation>Fisheries and Oceans Canada, Institute of Ocean Science, PO Box 9860, Sidney, British Columbia V8L 4B2, Canada</unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en" type="author"><keyword xml:id="kwd1">Cytochrome P4501A1</keyword><keyword xml:id="kwd2">Gene expression</keyword><keyword xml:id="kwd3">Polychlorinated biphenyls</keyword><keyword xml:id="kwd4">Dioxins</keyword><keyword xml:id="kwd5">Furans</keyword></keywordGroup><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Abstract</title><p>Adult Atlantic tomcod, <i>Microgadus tomcod</i>, from the Hudson River, New York State, USA, exhibit reduced inducibility of hepatic cytochrome P4501A1 (CYP1A1) mRNA compared with adult tomcod from the cleaner Miramichi River, New Brunswick, Canada, when treated with coplanar polychlorinated biphenyl (PCB) congeners or 2,3,7,8‐tetrachlorodibenzo‐<i>p</i>‐dioxin. In contrast, little difference in CYP1A1 inducibility is observed between tomcod from these two rivers when treated with polycyclic aromatic hydrocarbons (PAHs). We sought to determine if impaired hepatic CYP1A1 inducibility in Hudson River tomcod results from a multigenerational, genetic adaptation or a single generational, physiological acclimation. Embryos and larvae from controlled experimental crosses of Hudson River and Miramichi River parents were exposed for 24 h to water‐borne PCB congener 77 (10 ppm), benzo[<i>a</i>]pyrene (B<i>a</i>P; 10 ppm), or dimethysulfoxide, and CYP1A1 expression was assessed in individual larva using competitive reverse transcriptase polymerase chain reaction (RT‐PCR) analysis. The CYP1A1 mRNA was significantly induced in larvae from both populations by B<i>a</i>P (47‐ and 52‐fold) and PCB 77 (9‐ and 22‐fold), although levels of expression were higher in offspring of Miramichi matings. Most important, CYP1A1 mRNA was significantly induced by PCB 77 in larvae from Hudson River parents. Concentrations of dioxin, furan, and PCB congeners were measured in livers and eggs of female tomcod from these two locales to quantify the extent of maternal transfer of contaminants. For both rivers, wet‐weight contaminant concentrations were significantly higher (4–7 times) in livers than in eggs of the same females, suggesting that a threshold level of contaminants may have to be reached before CYP1A1 transcription is impaired. We conclude that reduced inducibility of hepatic CYP1A1 mRNA in adult tomcod from the Hudson River is most consistent with single‐generational acclimation.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>An evaluation of the etiology of reduced CYP1A1 messenger RNA expression in the Atlantic tomcod from the Hudson River, New York, USA, using reverse transcriptase polymerase chain reaction analysis</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>Etiology of reduced CYP1A1 mRNA expression in tomcod</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>An evaluation of the etiology of reduced CYP1A1 messenger RNA expression in the Atlantic tomcod from the Hudson River, New York, USA, using reverse transcriptase polymerase chain reaction analysis</title></titleInfo><name type="personal"><namePart type="given">Nirmal K.</namePart><namePart type="family">Roy</namePart><affiliation>Department of Environmental Medicine, New York University School of Medicine, 57 Old Forge Road, Tuxedo, New York 10987, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Simon</namePart><namePart type="family">Courtenay</namePart><affiliation>Fisheries and Oceans Canada, Gulf Fisheries Centre, PO Box 5030, Moncton, New Brunswick E1C 9B6, Canada</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Zhanpeng</namePart><namePart type="family">Yuan</namePart><affiliation>Department of Environmental Medicine, New York University School of Medicine, 57 Old Forge Road, Tuxedo, New York 10987, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Michael</namePart><namePart type="family">Ikonomou</namePart><affiliation>Fisheries and Oceans Canada, Institute of Ocean Science, PO Box 9860, Sidney, British Columbia V8L 4B2, Canada</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Isaac</namePart><namePart type="family">Wirgin</namePart><affiliation>Department of Environmental Medicine, New York University School of Medicine, 57 Old Forge Road, Tuxedo, New York 10987, USA</affiliation><affiliation>Department of Environmental Medicine, New York University School of Medicine, 57 Old Forge Road, Tuxedo, New York 10987, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Wiley Periodicals, Inc.</publisher><place><placeTerm type="text">Hoboken</placeTerm></place><dateIssued encoding="w3cdtf">2001-05</dateIssued><dateCaptured encoding="w3cdtf">2000-01-10</dateCaptured><dateValid encoding="w3cdtf">2000-09-19</dateValid><copyrightDate encoding="w3cdtf">2001</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">3</extent><extent unit="tables">1</extent><extent unit="references">40</extent></physicalDescription><abstract lang="en">Adult Atlantic tomcod, Microgadus tomcod, from the Hudson River, New York State, USA, exhibit reduced inducibility of hepatic cytochrome P4501A1 (CYP1A1) mRNA compared with adult tomcod from the cleaner Miramichi River, New Brunswick, Canada, when treated with coplanar polychlorinated biphenyl (PCB) congeners or 2,3,7,8‐tetrachlorodibenzo‐p‐dioxin. In contrast, little difference in CYP1A1 inducibility is observed between tomcod from these two rivers when treated with polycyclic aromatic hydrocarbons (PAHs). We sought to determine if impaired hepatic CYP1A1 inducibility in Hudson River tomcod results from a multigenerational, genetic adaptation or a single generational, physiological acclimation. Embryos and larvae from controlled experimental crosses of Hudson River and Miramichi River parents were exposed for 24 h to water‐borne PCB congener 77 (10 ppm), benzo[a]pyrene (BaP; 10 ppm), or dimethysulfoxide, and CYP1A1 expression was assessed in individual larva using competitive reverse transcriptase polymerase chain reaction (RT‐PCR) analysis. The CYP1A1 mRNA was significantly induced in larvae from both populations by BaP (47‐ and 52‐fold) and PCB 77 (9‐ and 22‐fold), although levels of expression were higher in offspring of Miramichi matings. Most important, CYP1A1 mRNA was significantly induced by PCB 77 in larvae from Hudson River parents. Concentrations of dioxin, furan, and PCB congeners were measured in livers and eggs of female tomcod from these two locales to quantify the extent of maternal transfer of contaminants. For both rivers, wet‐weight contaminant concentrations were significantly higher (4–7 times) in livers than in eggs of the same females, suggesting that a threshold level of contaminants may have to be reached before CYP1A1 transcription is impaired. We conclude that reduced inducibility of hepatic CYP1A1 mRNA in adult tomcod from the Hudson River is most consistent with single‐generational acclimation.</abstract><subject lang="en"><genre>keywords</genre><topic>Cytochrome P4501A1</topic><topic>Gene expression</topic><topic>Polychlorinated biphenyls</topic><topic>Dioxins</topic><topic>Furans</topic></subject><relatedItem type="host"><titleInfo><title>Environmental Toxicology and Chemistry</title><subTitle>An International Journal</subTitle></titleInfo><titleInfo type="abbreviated"><title>Environmental Toxicology and Chemistry</title></titleInfo><genre type="journal">journal</genre><subject><genre>article-category</genre><topic>Environmental Toxicology</topic></subject><identifier type="ISSN">0730-7268</identifier><identifier type="eISSN">1552-8618</identifier><identifier type="DOI">10.1002/(ISSN)1552-8618</identifier><identifier type="PublisherID">ETC</identifier><part><date>2001</date><detail type="volume"><caption>vol.</caption><number>20</number></detail><detail type="issue"><caption>no.</caption><number>5</number></detail><extent unit="pages"><start>1022</start><end>1030</end><total>9</total></extent></part></relatedItem><identifier type="istex">3539CE898630A1A56D588CE70846580FB7D553B8</identifier><identifier type="DOI">10.1002/etc.5620200512</identifier><identifier type="ArticleID">ETC5620200512</identifier><accessCondition type="use and reproduction" contentType="copyright">Copyright © 2001 SETAC</accessCondition><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>Wiley Periodicals, Inc.</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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