Temporal and spatial SOX9 expression patterns in the course of gonad development of the caudate amphibian Pleurodeles waltl
Identifieur interne : 001388 ( Istex/Corpus ); précédent : 001387; suivant : 001389Temporal and spatial SOX9 expression patterns in the course of gonad development of the caudate amphibian Pleurodeles waltl
Auteurs : Hélène Dumond ; Imane Al-Asaad ; Amand Chesnel ; Dominique Chardard ; Brigitte Boizet-Bonhoure ; Stéphane Flament ; Sandra KuntzSource :
- Journal of Experimental Zoology Part B: Molecular and Developmental Evolution [ 1552-5007 ] ; 2011-05-15.
Abstract
The SOX family of transcription factors is thought to regulate gene expression in a wide variety of developmental processes. Namely, SOX9 expression is conserved in vertebrate sex determination or differentiation. Nevertheless, information about caudate amphibians is lacking. In this study, we provide data on Pleurodeles waltl, a species that displays a ZZ/ZW genetic mode of sex determination and a temperature‐dependent mechanism of female‐to‐male sex reversal. Phylogenetic analysis of SOX9 P. waltl ortholog reveals that the deduced protein segregates from the group of anuran and could be more closely related to amniote than to anamniote. However, SOX9 lacks the PQA‐rich domain present in amniotes. In larvae, SOX9 is expressed in both sexes in gonad–mesonephros complexes as soon as stage 42, before gonad differentiation. At stage 5460d at which testis differentiation is already in progress, analyses of isolated gonads reveal a male‐enriched expression of SOX9, which was quantified by real‐time PCR. At the end of metamorphosis (stage 56), SOX9 shows a nuclear localization only in the testis. In adults, SOX9 is still expressed in testes and ovaries. In the ovary, SOX9 is found in oocytes from stage I to stage VI but it is never detected in the nucleus. Our results suggest that in P. waltl, like in non mammalian vertebrates, SOX9 could play a role during the late phase of gonad differentiation rather than in sex determination. Its role in germ cells of the adult ovary has still to be elucidated. J. Exp. Zool. (Mol. Dev. Evol.) 316:199–211, 2011. © 2010 Wiley‐Liss, Inc.
Url:
DOI: 10.1002/jez.b.21390
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In adults, SOX9 is still expressed in testes and ovaries. In the ovary, SOX9 is found in oocytes from stage I to stage VI but it is never detected in the nucleus. Our results suggest that in P. waltl, like in non mammalian vertebrates, SOX9 could play a role during the late phase of gonad differentiation rather than in sex determination. Its role in germ cells of the adult ovary has still to be elucidated. J. Exp. Zool. (Mol. Dev. 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caudate amphibian Pleurodeles waltl</title><author xml:id="author-1"><persName><forename type="first">Hélène</forename><surname>Dumond</surname></persName><note type="biography">Hélène Dumond and Imane Al‐Asaad contributed equally to this work.</note><affiliation>Hélène Dumond and Imane Al‐Asaad contributed equally to this work.</affiliation><affiliation>EA 4421: Signalisation Genomique et Recherche Translationnelle en Oncologie, Nancy‐Université, Nancy, France</affiliation></author><author xml:id="author-2"><persName><forename type="first">Imane</forename><surname>Al‐Asaad</surname></persName><note type="biography">Hélène Dumond and Imane Al‐Asaad contributed equally to this work.</note><affiliation>Hélène Dumond and Imane Al‐Asaad contributed equally to this work.</affiliation><affiliation>EA 4421: Signalisation Genomique et Recherche Translationnelle en Oncologie, Nancy‐Université, Nancy, France</affiliation></author><author xml:id="author-3"><persName><forename 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type="pageFirst">199</numbering><numbering type="pageLast">211</numbering></numberingGroup><correspondenceTo>Faculté des Sciences, Nancy‐Université, EA4421: SIGRETO, Boulevard des Aiguillettes, BP 239, 54506 Vandœuvre‐lès‐Nancy cedex, France===</correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:JEZ.JEZ21390.pdf"></link></linkGroup></publicationMeta><contentMeta><countGroup><count type="figureTotal" number="6"></count><count type="tableTotal" number="1"></count><count type="referenceTotal" number="47"></count></countGroup><titleGroup><title type="main" xml:lang="en">Temporal and spatial <i>SOX9</i> expression patterns in the course of gonad development of the caudate amphibian <i>Pleurodeles waltl</i></title><title type="short" xml:lang="en"><fi>SOX9</fi> EXPRESSION PROFILE IN <fi>Pleurodeles waltl</fi></title></titleGroup><creators><creator xml:id="au1" creatorRole="author" affiliationRef="#af1" noteRef="#fn1"><personName><givenNames>Hélène</givenNames><familyName>Dumond</familyName></personName></creator><creator xml:id="au2" creatorRole="author" affiliationRef="#af1" noteRef="#fn1"><personName><givenNames>Imane</givenNames><familyName>Al‐Asaad</familyName></personName></creator><creator xml:id="au3" creatorRole="author" affiliationRef="#af1"><personName><givenNames>Amand</givenNames><familyName>Chesnel</familyName></personName></creator><creator xml:id="au4" creatorRole="author" affiliationRef="#af2"><personName><givenNames>Dominique</givenNames><familyName>Chardard</familyName></personName></creator><creator xml:id="au5" creatorRole="author" affiliationRef="#af2"><personName><givenNames>Brigitte</givenNames><familyName>Boizet‐Bonhoure</familyName></personName></creator><creator xml:id="au6" creatorRole="author" affiliationRef="#af1" corresponding="yes"><personName><givenNames>Stéphane</givenNames><familyName>Flament</familyName></personName><contactDetails><email normalForm="stephane.flament@scbiol.uhp-nancy.fr">stephane.flament@scbiol.uhp‐nancy.fr</email></contactDetails></creator><creator xml:id="au7" creatorRole="author" affiliationRef="#af1"><personName><givenNames>Sandra</givenNames><familyName>Kuntz</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="af1" countryCode="FR" type="organization"><unparsedAffiliation>EA 4421: Signalisation Genomique et Recherche Translationnelle en Oncologie, Nancy‐Université, Nancy, France</unparsedAffiliation></affiliation><affiliation xml:id="af2" countryCode="FR" type="organization"><unparsedAffiliation>Department of Genetics and Development, Institut de Génétique Humaine, CNRS UPR1142, Montpellier, Cedex, France</unparsedAffiliation></affiliation></affiliationGroup><fundingInfo><fundingAgency>Université Henri Poincaré (BQR)</fundingAgency></fundingInfo><fundingInfo><fundingAgency>Ligue contre le Cancer</fundingAgency></fundingInfo><fundingInfo><fundingAgency>Conseil Régional de Lorraine</fundingAgency></fundingInfo><fundingInfo><fundingAgency>Nancy‐Université</fundingAgency></fundingInfo><fundingInfo><fundingAgency>Association pour la Recherche sur le Cancer</fundingAgency></fundingInfo><fundingInfo><fundingAgency>FEDER</fundingAgency></fundingInfo><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Abstract</title><p>The SOX family of transcription factors is thought to regulate gene expression in a wide variety of developmental processes. Namely, SOX9 expression is conserved in vertebrate sex determination or differentiation. Nevertheless, information about caudate amphibians is lacking. In this study, we provide data on <i>Pleurodeles waltl</i>, a species that displays a ZZ/ZW genetic mode of sex determination and a temperature‐dependent mechanism of female‐to‐male sex reversal. Phylogenetic analysis of <i>SOX9 P. waltl</i> ortholog reveals that the deduced protein segregates from the group of anuran and could be more closely related to amniote than to anamniote. However, SOX9 lacks the PQA‐rich domain present in amniotes. In larvae, <i>SOX9</i> is expressed in both sexes in gonad–mesonephros complexes as soon as stage 42, before gonad differentiation. At stage 54<sup>60d</sup> at which testis differentiation is already in progress, analyses of isolated gonads reveal a male‐enriched expression of <i>SOX9</i>, which was quantified by real‐time PCR. At the end of metamorphosis (stage 56), SOX9 shows a nuclear localization only in the testis. In adults, SOX9 is still expressed in testes and ovaries. In the ovary, SOX9 is found in oocytes from stage I to stage VI but it is never detected in the nucleus. Our results suggest that in <i>P. waltl</i>, like in non mammalian vertebrates, SOX9 could play a role during the late phase of gonad differentiation rather than in sex determination. Its role in germ cells of the adult ovary has still to be elucidated. <i>J. Exp. Zool. (Mol. Dev. Evol.) 316:199–211, 2011</i>. © 2010 Wiley‐Liss, Inc.</p></abstract></abstractGroup></contentMeta><noteGroup><note xml:id="fn1"><p>Hélène Dumond and Imane Al‐Asaad contributed equally to this work.</p></note></noteGroup></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Temporal and spatial SOX9 expression patterns in the course of gonad development of the caudate amphibian Pleurodeles waltl</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>SOX9 EXPRESSION PROFILE IN Pleurodeles waltl</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Temporal and spatial SOX9 expression patterns in the course of gonad development of the caudate amphibian Pleurodeles waltl</title></titleInfo><name type="personal"><namePart type="given">Hélène</namePart><namePart type="family">Dumond</namePart><affiliation>EA 4421: Signalisation Genomique et Recherche Translationnelle en Oncologie, Nancy‐Université, Nancy, France</affiliation><description>Hélène Dumond and Imane Al‐Asaad contributed equally to this work.</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Imane</namePart><namePart type="family">Al‐Asaad</namePart><affiliation>EA 4421: Signalisation Genomique et Recherche Translationnelle en Oncologie, Nancy‐Université, Nancy, France</affiliation><description>Hélène Dumond and Imane Al‐Asaad contributed equally to this work.</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Amand</namePart><namePart type="family">Chesnel</namePart><affiliation>EA 4421: Signalisation Genomique et Recherche Translationnelle en Oncologie, Nancy‐Université, Nancy, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Dominique</namePart><namePart type="family">Chardard</namePart><affiliation>Department of Genetics and Development, Institut de Génétique Humaine, CNRS UPR1142, Montpellier, Cedex, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Brigitte</namePart><namePart type="family">Boizet‐Bonhoure</namePart><affiliation>Department of Genetics and Development, Institut de Génétique Humaine, CNRS UPR1142, Montpellier, Cedex, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Stéphane</namePart><namePart type="family">Flament</namePart><affiliation>EA 4421: Signalisation Genomique et Recherche Translationnelle en Oncologie, Nancy‐Université, Nancy, France</affiliation><affiliation>Faculté des Sciences, Nancy‐Université, EA4421: SIGRETO, Boulevard des Aiguillettes, BP 239, 54506 Vandœuvre‐lès‐Nancy cedex, France===</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Sandra</namePart><namePart type="family">Kuntz</namePart><affiliation>EA 4421: Signalisation Genomique et Recherche Translationnelle en Oncologie, Nancy‐Université, Nancy, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><place><placeTerm type="text">Hoboken</placeTerm></place><dateIssued encoding="w3cdtf">2011-05-15</dateIssued><dateCaptured encoding="w3cdtf">2010-06-16</dateCaptured><dateValid encoding="w3cdtf">2010-10-27</dateValid><copyrightDate encoding="w3cdtf">2011</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">6</extent><extent unit="tables">1</extent><extent unit="references">47</extent></physicalDescription><abstract lang="en">The SOX family of transcription factors is thought to regulate gene expression in a wide variety of developmental processes. Namely, SOX9 expression is conserved in vertebrate sex determination or differentiation. Nevertheless, information about caudate amphibians is lacking. In this study, we provide data on Pleurodeles waltl, a species that displays a ZZ/ZW genetic mode of sex determination and a temperature‐dependent mechanism of female‐to‐male sex reversal. Phylogenetic analysis of SOX9 P. waltl ortholog reveals that the deduced protein segregates from the group of anuran and could be more closely related to amniote than to anamniote. However, SOX9 lacks the PQA‐rich domain present in amniotes. In larvae, SOX9 is expressed in both sexes in gonad–mesonephros complexes as soon as stage 42, before gonad differentiation. At stage 5460d at which testis differentiation is already in progress, analyses of isolated gonads reveal a male‐enriched expression of SOX9, which was quantified by real‐time PCR. At the end of metamorphosis (stage 56), SOX9 shows a nuclear localization only in the testis. In adults, SOX9 is still expressed in testes and ovaries. In the ovary, SOX9 is found in oocytes from stage I to stage VI but it is never detected in the nucleus. Our results suggest that in P. waltl, like in non mammalian vertebrates, SOX9 could play a role during the late phase of gonad differentiation rather than in sex determination. Its role in germ cells of the adult ovary has still to be elucidated. J. Exp. Zool. (Mol. Dev. Evol.) 316:199–211, 2011. © 2010 Wiley‐Liss, Inc.</abstract><note type="funding">Université Henri Poincaré (BQR)</note><note type="funding">Ligue contre le Cancer</note><note type="funding">Conseil Régional de Lorraine</note><note type="funding">Nancy‐Université</note><note type="funding">Association pour la Recherche sur le Cancer</note><note type="funding">FEDER</note><relatedItem type="host"><titleInfo><title>Journal of Experimental Zoology Part B: Molecular and Developmental Evolution</title></titleInfo><titleInfo type="abbreviated"><title>J. Exp. Zool.</title></titleInfo><genre type="journal">journal</genre><subject><genre>article-category</genre><topic>Research Article</topic></subject><identifier type="ISSN">1552-5007</identifier><identifier type="eISSN">1552-5015</identifier><identifier type="DOI">10.1002/(ISSN)1552-5015</identifier><identifier type="PublisherID">JEZ</identifier><part><date>2011</date><detail type="volume"><caption>vol.</caption><number>316B</number></detail><detail type="issue"><caption>no.</caption><number>3</number></detail><extent unit="pages"><start>199</start><end>211</end><total>13</total></extent></part></relatedItem><relatedItem type="preceding"><titleInfo><title>Journal of Experimental Zoology</title></titleInfo><identifier type="ISSN">0022-104X</identifier><identifier type="ISSN">1097-010X</identifier><part><date point="end">2004</date></part></relatedItem><identifier type="istex">EE56B6F819AED85F42399B23CDC00CE6AB0DF70E</identifier><identifier type="DOI">10.1002/jez.b.21390</identifier><identifier type="ArticleID">JEZ21390</identifier><accessCondition type="use and reproduction" contentType="copyright">Copyright © 2010 Wiley‐Liss, Inc., A Wiley Company</accessCondition><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>Wiley Subscription Services, Inc., A Wiley Company</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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