Sex assignment of lake sturgeon (Acipenser fluvescens) based on plasma sex hormone and vitellogenin levels
Identifieur interne : 001341 ( Istex/Corpus ); précédent : 001340; suivant : 001342Sex assignment of lake sturgeon (Acipenser fluvescens) based on plasma sex hormone and vitellogenin levels
Auteurs : J. M. Craig ; D. M. Papoulias ; M. V. Thomas ; M. L. Annis ; J. BoaseSource :
- Journal of Applied Ichthyology [ 0175-8659 ] ; 2009-10.
Abstract
This study focused on identifying the sex of lake sturgeon by measuring the sex hormones estradiol and testosterone, and the phosphoprotein vitellogenin (Vtg) in blood plasma by radioimmunoassay and enzyme‐linked immunosorbent assay, respectively, and evaluating these techniques as tools in lake sturgeon population management. Surveys of the St Clair River (SCR) lake sturgeon population have characterized it as rebounding by having steady or increasing recruitment since 1997. However, researchers have not been able to effectively determine the sex for most of the sturgeon they capture because few fish caught during surveys are releasing gametes. A total of 115 fish were sampled from May through June in 2004 and 2005 from the SCR, Michigan, USA. Of these, only four females and eight males were verified (i.e. they were releasing gametes at time of capture), resulting in very few fish with which to validate blood hormone and Vtg biomarkers of sex. Fifty‐six percent of the fish were assigned a sex designation based on biomarker criteria. Correspondence between actual gonadal sex and biomarker‐directed classification was good for the small subset of fish for which gonadal sex was definitively determined. Moreover, application of the steroid values in a predictive sex assignment model developed for white sturgeon misclassified only the same two fish that were misclassified with the steroid and Vtg biomarkers. The experimental results suggest a sex ratio of 1 : 2.7 (F:M), however more conclusive methods are needed to confirm this ratio because so few fish were available for sex validation. Of the 43 males, 14 were within the legal slot limit, 11 were smaller than 1067 mm total length (TL), and 18 were larger than 1270 mm TL. All 15 females were larger than 1270 mm TL, and thus protected by the slot limit criteria. Considering that lake sturgeon are threatened in Michigan, an advantage to using blood plasma assays was that fish were not harmed, and sample collection was quick, simple, and inexpensive. However, because a sufficiently large number of fish could not be validated for gonadal sex due to handling restrictions given the fish’s protected status, assignment of sex is not based on a robust multi‐variate model. An immediate alternative may be to use other non‐invasive field methods (e.g. ultrasound, fiber‐optic endoscope) to provide a more timely classification while establishing well‐validated plasma hormone and Vtg‐based predictive models for sex assignment of lake sturgeon.
Url:
DOI: 10.1111/j.1439-0426.2009.01289.x
Links to Exploration step
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<front><div type="abstract" xml:lang="en">This study focused on identifying the sex of lake sturgeon by measuring the sex hormones estradiol and testosterone, and the phosphoprotein vitellogenin (Vtg) in blood plasma by radioimmunoassay and enzyme‐linked immunosorbent assay, respectively, and evaluating these techniques as tools in lake sturgeon population management. Surveys of the St Clair River (SCR) lake sturgeon population have characterized it as rebounding by having steady or increasing recruitment since 1997. However, researchers have not been able to effectively determine the sex for most of the sturgeon they capture because few fish caught during surveys are releasing gametes. A total of 115 fish were sampled from May through June in 2004 and 2005 from the SCR, Michigan, USA. Of these, only four females and eight males were verified (i.e. they were releasing gametes at time of capture), resulting in very few fish with which to validate blood hormone and Vtg biomarkers of sex. Fifty‐six percent of the fish were assigned a sex designation based on biomarker criteria. Correspondence between actual gonadal sex and biomarker‐directed classification was good for the small subset of fish for which gonadal sex was definitively determined. Moreover, application of the steroid values in a predictive sex assignment model developed for white sturgeon misclassified only the same two fish that were misclassified with the steroid and Vtg biomarkers. The experimental results suggest a sex ratio of 1 : 2.7 (F:M), however more conclusive methods are needed to confirm this ratio because so few fish were available for sex validation. Of the 43 males, 14 were within the legal slot limit, 11 were smaller than 1067 mm total length (TL), and 18 were larger than 1270 mm TL. All 15 females were larger than 1270 mm TL, and thus protected by the slot limit criteria. Considering that lake sturgeon are threatened in Michigan, an advantage to using blood plasma assays was that fish were not harmed, and sample collection was quick, simple, and inexpensive. However, because a sufficiently large number of fish could not be validated for gonadal sex due to handling restrictions given the fish’s protected status, assignment of sex is not based on a robust multi‐variate model. An immediate alternative may be to use other non‐invasive field methods (e.g. ultrasound, fiber‐optic endoscope) to provide a more timely classification while establishing well‐validated plasma hormone and Vtg‐based predictive models for sex assignment of lake sturgeon.</div>
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<correspondenceTo><b>Author’s address:</b>
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<affiliation xml:id="a4" countryCode="US"><unparsedAffiliation>United States Fish & Wildlife Service, Alpena Fishery Resources Office, Alpena, MI, USA</unparsedAffiliation>
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<abstractGroup><abstract type="main" xml:lang="en"><title type="main">Summary</title>
<p>This study focused on identifying the sex of lake sturgeon by measuring the sex hormones estradiol and testosterone, and the phosphoprotein vitellogenin (Vtg) in blood plasma by radioimmunoassay and enzyme‐linked immunosorbent assay, respectively, and evaluating these techniques as tools in lake sturgeon population management. Surveys of the St Clair River (SCR) lake sturgeon population have characterized it as rebounding by having steady or increasing recruitment since 1997. However, researchers have not been able to effectively determine the sex for most of the sturgeon they capture because few fish caught during surveys are releasing gametes. A total of 115 fish were sampled from May through June in 2004 and 2005 from the SCR, Michigan, USA. Of these, only four females and eight males were verified (i.e. they were releasing gametes at time of capture), resulting in very few fish with which to validate blood hormone and Vtg biomarkers of sex. Fifty‐six percent of the fish were assigned a sex designation based on biomarker criteria. Correspondence between actual gonadal sex and biomarker‐directed classification was good for the small subset of fish for which gonadal sex was definitively determined. Moreover, application of the steroid values in a predictive sex assignment model developed for white sturgeon misclassified only the same two fish that were misclassified with the steroid and Vtg biomarkers. The experimental results suggest a sex ratio of 1 : 2.7 (F:M), however more conclusive methods are needed to confirm this ratio because so few fish were available for sex validation. Of the 43 males, 14 were within the legal slot limit, 11 were smaller than 1067 mm total length (TL), and 18 were larger than 1270 mm TL. All 15 females were larger than 1270 mm TL, and thus protected by the slot limit criteria. Considering that lake sturgeon are threatened in Michigan, an advantage to using blood plasma assays was that fish were not harmed, and sample collection was quick, simple, and inexpensive. However, because a sufficiently large number of fish could not be validated for gonadal sex due to handling restrictions given the fish’s protected status, assignment of sex is not based on a robust multi‐variate model. An immediate alternative may be to use other non‐invasive field methods (e.g. ultrasound, fiber‐optic endoscope) to provide a more timely classification while establishing well‐validated plasma hormone and Vtg‐based predictive models for sex assignment of lake sturgeon.</p>
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<mods version="3.6"><titleInfo lang="en"><title>Sex assignment of lake sturgeon (Acipenser fluvescens) based on plasma sex hormone and vitellogenin levels</title>
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<titleInfo type="abbreviated" lang="en"><title>Lake sturgeon sex hormones</title>
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<titleInfo type="alternative" contentType="CDATA" lang="en"><title>Sex assignment of lake sturgeon (Acipenser fluvescens) based on plasma sex hormone and vitellogenin levels</title>
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<name type="personal"><namePart type="given">J. M.</namePart>
<namePart type="family">Craig</namePart>
<affiliation>United States Geological Survey, Great Lakes Science Center, Ann Arbor, MI, USA</affiliation>
<role><roleTerm type="text">author</roleTerm>
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</name>
<name type="personal"><namePart type="given">D. M.</namePart>
<namePart type="family">Papoulias</namePart>
<affiliation>United States Geological Survey, Columbia Environmental Research Center, Columbia, MO, USA</affiliation>
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<name type="personal"><namePart type="given">M. V.</namePart>
<namePart type="family">Thomas</namePart>
<affiliation>Michigan Department of Natural Resources, Lake St Clair Fisheries Research Station, Mt. Clemens, MI, USA</affiliation>
<role><roleTerm type="text">author</roleTerm>
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<name type="personal"><namePart type="given">M. L.</namePart>
<namePart type="family">Annis</namePart>
<affiliation>United States Geological Survey, Columbia Environmental Research Center, Columbia, MO, USA</affiliation>
<role><roleTerm type="text">author</roleTerm>
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<name type="personal"><namePart type="given">J.</namePart>
<namePart type="family">Boase</namePart>
<affiliation>United States Fish & Wildlife Service, Alpena Fishery Resources Office, Alpena, MI, USA</affiliation>
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<originInfo><publisher>Blackwell Publishing Ltd</publisher>
<place><placeTerm type="text">Oxford, UK</placeTerm>
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<dateIssued encoding="w3cdtf">2009-10</dateIssued>
<edition>Received: January 8, 2009 Accepted: February 28, 2009</edition>
<copyrightDate encoding="w3cdtf">2009</copyrightDate>
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<languageTerm type="code" authority="iso639-2b">eng</languageTerm>
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<abstract lang="en">This study focused on identifying the sex of lake sturgeon by measuring the sex hormones estradiol and testosterone, and the phosphoprotein vitellogenin (Vtg) in blood plasma by radioimmunoassay and enzyme‐linked immunosorbent assay, respectively, and evaluating these techniques as tools in lake sturgeon population management. Surveys of the St Clair River (SCR) lake sturgeon population have characterized it as rebounding by having steady or increasing recruitment since 1997. However, researchers have not been able to effectively determine the sex for most of the sturgeon they capture because few fish caught during surveys are releasing gametes. A total of 115 fish were sampled from May through June in 2004 and 2005 from the SCR, Michigan, USA. Of these, only four females and eight males were verified (i.e. they were releasing gametes at time of capture), resulting in very few fish with which to validate blood hormone and Vtg biomarkers of sex. Fifty‐six percent of the fish were assigned a sex designation based on biomarker criteria. Correspondence between actual gonadal sex and biomarker‐directed classification was good for the small subset of fish for which gonadal sex was definitively determined. Moreover, application of the steroid values in a predictive sex assignment model developed for white sturgeon misclassified only the same two fish that were misclassified with the steroid and Vtg biomarkers. The experimental results suggest a sex ratio of 1 : 2.7 (F:M), however more conclusive methods are needed to confirm this ratio because so few fish were available for sex validation. Of the 43 males, 14 were within the legal slot limit, 11 were smaller than 1067 mm total length (TL), and 18 were larger than 1270 mm TL. All 15 females were larger than 1270 mm TL, and thus protected by the slot limit criteria. Considering that lake sturgeon are threatened in Michigan, an advantage to using blood plasma assays was that fish were not harmed, and sample collection was quick, simple, and inexpensive. However, because a sufficiently large number of fish could not be validated for gonadal sex due to handling restrictions given the fish’s protected status, assignment of sex is not based on a robust multi‐variate model. An immediate alternative may be to use other non‐invasive field methods (e.g. ultrasound, fiber‐optic endoscope) to provide a more timely classification while establishing well‐validated plasma hormone and Vtg‐based predictive models for sex assignment of lake sturgeon.</abstract>
<relatedItem type="host"><titleInfo><title>Journal of Applied Ichthyology</title>
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<genre type="journal">journal</genre>
<identifier type="ISSN">0175-8659</identifier>
<identifier type="eISSN">1439-0426</identifier>
<identifier type="DOI">10.1111/(ISSN)1439-0426</identifier>
<identifier type="PublisherID">JAI</identifier>
<part><date>2009</date>
<detail type="title"><title>Proceedings of the Inaugural Meeting of the North American Chapter of the World Sturgeon Conservation Society, Ottawa, Canada, August 19‐21, 2008</title>
</detail>
<detail type="volume"><caption>vol.</caption>
<number>25</number>
</detail>
<detail type="supplement"><caption>Suppl. no.</caption>
<number>s2</number>
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<extent unit="pages"><start>60</start>
<end>67</end>
<total>8</total>
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<identifier type="DOI">10.1111/j.1439-0426.2009.01289.x</identifier>
<identifier type="ArticleID">JAI1289</identifier>
<accessCondition type="use and reproduction" contentType="copyright">No claim to original US government works. Journal compilation © 2009 Blackwell Verlag, Berlin</accessCondition>
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