Distribution of choline acetyltransferase immunoreactivity in the brain of an elasmobranch, the lesser spotted dogfish (Scyliorhinus canicula)
Identifieur interne : 001333 ( Istex/Corpus ); précédent : 001332; suivant : 001334Distribution of choline acetyltransferase immunoreactivity in the brain of an elasmobranch, the lesser spotted dogfish (Scyliorhinus canicula)
Auteurs : Ram N Anad N ; Pilar Molist ; Isabel Rodríguez-Moldes ; Jesús María L Pez ; Inés Quintela ; María Carmen Cervi O ; Primitivo Barja ; Agustín GonzálezSource :
- Journal of Comparative Neurology [ 0021-9967 ] ; 2000-05-01.
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- KwdEn :
Abstract
Although the distribution of cholinergic cells is remarkably similar across the vertebrate species, no data are available on more primitive species, such as cartilaginous fishes. To extend the evolutionary analysis of the cholinergic systems, we studied the distribution of cholinergic neurons in the brain and rostral spinal cord of Scyliorhinus canicula by immunocytochemistry using an antibody against the enzyme choline acetyltransferase (ChAT). Western blot analysis of brain extracts of dogfish, sturgeon, trout, and rat showed that this antibody recognized similar bands in the four species. Putative cholinergic neurons were observed in most brain regions, including the telencephalon, diencephalon, cerebellum, and brainstem. In the retrobulbar region and superficial dorsal pallium of the telencephalon, numerous small pallial cells were ChAT‐like immunoreactive. In addition, tufted cells of the olfactory bulb and some cells in the lateral pallium showed faint immunoreactivity. In the preoptic‐hypothalamic region, ChAT‐immunoreactive (ChAT‐ir) cells were found in the preoptic nucleus, the vascular organ of the terminal lamina, and a small population in the caudal tuber. In the epithalamus, the pineal photoreceptors were intensely positive. Many cells of the habenula were faintly ChAT‐ir, but the neuropil of the interpeduncular nucleus showed intense ChAT immunoreactivity. In the pretectal region, ChAT‐ir cells were observed only in the superficial pretectal nucleus. In the brainstem, the somatomotor and branchiomotor nuclei, the octavolateral efferent nucleus, and a cell group just rostral to the Edinger‐Westphal (EW) nucleus contained ChAT‐ir neurons. In addition, the trigeminal mesencephalic nucleus, the nucleus G of the isthmus, some locus coeruleus cells, and some cell populations of the vestibular nuclei and of the electroreceptive nucleus of the octavolateral region exhibited ChAT immunoreactivity. In the reticular areas of the brainstem, the nucleus of the medial longitudinal fascicle, many reticular neurons of the rhombencephalon, and cells of the nucleus of the lateral funiculus were immunoreactive to this antibody. In the cerebellum, Golgi cells of the granule cell layer and some cells of the cerebellar nucleus were also ChAT‐ir. In the rostral spinal cord, ChAT immunoreactivity was observed in cells of the motor column, the dorsal horn, the marginal nucleus (a putative stretch‐receptor organ), and in interstitial cells of the ventral funiculus. These results demonstrate for the first time that cholinergic neurons are distributed widely in the central nervous system of elasmobranchs and that their cholinergic systems have evolved several characteristics that are unique to this group. J. Comp. Neurol. 420:139–170, 2000. © 2000 Wiley‐Liss, Inc.
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DOI: 10.1002/(SICI)1096-9861(20000501)420:2<139::AID-CNE1>3.0.CO;2-T
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canicula)</title><author><name sortKey="Anad N, Ram N" sort="Anad N, Ram N" uniqKey="Anad N R" first="Ram N" last="Anad N">Ram N Anad N</name><affiliation><mods:affiliation>Department of Fundamental Biology, University of Santiago de Compostela, 15706‐Santiago de Compostela, Spain</mods:affiliation></affiliation><affiliation><mods:affiliation>Department of Fundamental Biology, University of Santiago de Compostela, 15706‐Santiago de Compostela, Spain</mods:affiliation></affiliation></author><author><name sortKey="Molist, Pilar" sort="Molist, Pilar" uniqKey="Molist P" first="Pilar" last="Molist">Pilar Molist</name><affiliation><mods:affiliation>Department of Functional Biology and Health Sciences, University of Vigo, 36200‐Vigo, Spain</mods:affiliation></affiliation></author><author><name sortKey="Rodriguez Oldes, Isabel" sort="Rodriguez Oldes, Isabel" uniqKey="Rodriguez Oldes I" first="Isabel" last="Rodríguez-Moldes">Isabel Rodríguez-Moldes</name><affiliation><mods:affiliation>Department of Fundamental Biology, University of Santiago de Compostela, 15706‐Santiago de Compostela, Spain</mods:affiliation></affiliation></author><author><name sortKey="L Pez, Jesus Maria" sort="L Pez, Jesus Maria" uniqKey="L Pez J" first="Jesús María" last="L Pez">Jesús María L Pez</name><affiliation><mods:affiliation>Department of Cell Biology, University Complutense of Madrid, 28040‐Madrid, Spain</mods:affiliation></affiliation></author><author><name sortKey="Quintela, Ines" sort="Quintela, Ines" uniqKey="Quintela I" first="Inés" last="Quintela">Inés Quintela</name><affiliation><mods:affiliation>Department of Fundamental Biology, University of Santiago de Compostela, 15706‐Santiago de Compostela, Spain</mods:affiliation></affiliation><affiliation><mods:affiliation>Department of Biochemistry and Molecular Biology, University of Santiago de Compostela, 15706‐Santiago de Compostela, Spain</mods:affiliation></affiliation></author><author><name sortKey="Cervi O, Maria Carmen" sort="Cervi O, Maria Carmen" uniqKey="Cervi O M" first="María Carmen" last="Cervi O">María Carmen Cervi O</name><affiliation><mods:affiliation>Department of Biochemistry and Molecular Biology, University of Santiago de Compostela, 15706‐Santiago de Compostela, Spain</mods:affiliation></affiliation></author><author><name sortKey="Barja, Primitivo" sort="Barja, Primitivo" uniqKey="Barja P" first="Primitivo" last="Barja">Primitivo Barja</name><affiliation><mods:affiliation>Department of Biochemistry and Molecular Biology, University of Santiago de Compostela, 15706‐Santiago de Compostela, Spain</mods:affiliation></affiliation></author><author><name sortKey="Gonzalez, Agustin" sort="Gonzalez, Agustin" uniqKey="Gonzalez A" first="Agustín" last="González">Agustín González</name><affiliation><mods:affiliation>Department of Cell Biology, University Complutense of Madrid, 28040‐Madrid, Spain</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Comparative Neurology</title><title level="j" type="abbrev">J. 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Neurol.</title><idno type="ISSN">0021-9967</idno><idno type="eISSN">1096-9861</idno><imprint><publisher>John Wiley & Sons, Inc.</publisher><pubPlace>New York</pubPlace><date type="published" when="2000-05-01">2000-05-01</date><biblScope unit="volume">420</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="139">139</biblScope><biblScope unit="page" to="170">170</biblScope></imprint><idno type="ISSN">0021-9967</idno></series><idno type="istex">5E0DCCC0DB7DC2395E94A4966E236310AC63C739</idno><idno type="DOI">10.1002/(SICI)1096-9861(20000501)420:2<139::AID-CNE1>3.0.CO;2-T</idno><idno type="ArticleID">CNE1</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0021-9967</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>acetylcholine</term><term>brain</term><term>dogfish</term><term>elasmobranchs</term><term>immunoblotting</term><term>immunohistochemistry</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Although the distribution of cholinergic cells is remarkably similar across the vertebrate species, no data are available on more primitive species, such as cartilaginous fishes. To extend the evolutionary analysis of the cholinergic systems, we studied the distribution of cholinergic neurons in the brain and rostral spinal cord of Scyliorhinus canicula by immunocytochemistry using an antibody against the enzyme choline acetyltransferase (ChAT). Western blot analysis of brain extracts of dogfish, sturgeon, trout, and rat showed that this antibody recognized similar bands in the four species. Putative cholinergic neurons were observed in most brain regions, including the telencephalon, diencephalon, cerebellum, and brainstem. In the retrobulbar region and superficial dorsal pallium of the telencephalon, numerous small pallial cells were ChAT‐like immunoreactive. In addition, tufted cells of the olfactory bulb and some cells in the lateral pallium showed faint immunoreactivity. In the preoptic‐hypothalamic region, ChAT‐immunoreactive (ChAT‐ir) cells were found in the preoptic nucleus, the vascular organ of the terminal lamina, and a small population in the caudal tuber. In the epithalamus, the pineal photoreceptors were intensely positive. Many cells of the habenula were faintly ChAT‐ir, but the neuropil of the interpeduncular nucleus showed intense ChAT immunoreactivity. In the pretectal region, ChAT‐ir cells were observed only in the superficial pretectal nucleus. In the brainstem, the somatomotor and branchiomotor nuclei, the octavolateral efferent nucleus, and a cell group just rostral to the Edinger‐Westphal (EW) nucleus contained ChAT‐ir neurons. In addition, the trigeminal mesencephalic nucleus, the nucleus G of the isthmus, some locus coeruleus cells, and some cell populations of the vestibular nuclei and of the electroreceptive nucleus of the octavolateral region exhibited ChAT immunoreactivity. In the reticular areas of the brainstem, the nucleus of the medial longitudinal fascicle, many reticular neurons of the rhombencephalon, and cells of the nucleus of the lateral funiculus were immunoreactive to this antibody. In the cerebellum, Golgi cells of the granule cell layer and some cells of the cerebellar nucleus were also ChAT‐ir. In the rostral spinal cord, ChAT immunoreactivity was observed in cells of the motor column, the dorsal horn, the marginal nucleus (a putative stretch‐receptor organ), and in interstitial cells of the ventral funiculus. These results demonstrate for the first time that cholinergic neurons are distributed widely in the central nervous system of elasmobranchs and that their cholinergic systems have evolved several characteristics that are unique to this group. J. Comp. Neurol. 420:139–170, 2000. © 2000 Wiley‐Liss, Inc.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>Ramón Anadón</name><affiliations><json:string>Department of Fundamental Biology, University of Santiago de Compostela, 15706‐Santiago de Compostela, Spain</json:string><json:string>Department of Fundamental Biology, University of Santiago de Compostela, 15706‐Santiago de Compostela, Spain</json:string></affiliations></json:item><json:item><name>Pilar Molist</name><affiliations><json:string>Department of Functional Biology and Health Sciences, University of Vigo, 36200‐Vigo, Spain</json:string></affiliations></json:item><json:item><name>Isabel Rodríguez‐Moldes</name><affiliations><json:string>Department of Fundamental Biology, University of Santiago de Compostela, 15706‐Santiago de Compostela, Spain</json:string></affiliations></json:item><json:item><name>Jesús María López</name><affiliations><json:string>Department of Cell Biology, University Complutense of Madrid, 28040‐Madrid, Spain</json:string></affiliations></json:item><json:item><name>Inés Quintela</name><affiliations><json:string>Department of Fundamental Biology, University of Santiago de Compostela, 15706‐Santiago de Compostela, Spain</json:string><json:string>Department of Biochemistry and Molecular Biology, University of Santiago de Compostela, 15706‐Santiago de Compostela, Spain</json:string></affiliations></json:item><json:item><name>María Carmen Cerviño</name><affiliations><json:string>Department of Biochemistry and Molecular Biology, University of Santiago de Compostela, 15706‐Santiago de Compostela, Spain</json:string></affiliations></json:item><json:item><name>Primitivo Barja</name><affiliations><json:string>Department of Biochemistry and Molecular Biology, University of Santiago de Compostela, 15706‐Santiago de Compostela, Spain</json:string></affiliations></json:item><json:item><name>Agustín González</name><affiliations><json:string>Department of Cell Biology, University Complutense of Madrid, 28040‐Madrid, Spain</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>acetylcholine</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>brain</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>immunohistochemistry</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>immunoblotting</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>dogfish</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>elasmobranchs</value></json:item></subject><articleId><json:string>CNE1</json:string></articleId><language><json:string>eng</json:string></language><originalGenre><json:string>article</json:string></originalGenre><abstract>Although the distribution of cholinergic cells is remarkably similar across the vertebrate species, no data are available on more primitive species, such as cartilaginous fishes. To extend the evolutionary analysis of the cholinergic systems, we studied the distribution of cholinergic neurons in the brain and rostral spinal cord of Scyliorhinus canicula by immunocytochemistry using an antibody against the enzyme choline acetyltransferase (ChAT). Western blot analysis of brain extracts of dogfish, sturgeon, trout, and rat showed that this antibody recognized similar bands in the four species. Putative cholinergic neurons were observed in most brain regions, including the telencephalon, diencephalon, cerebellum, and brainstem. In the retrobulbar region and superficial dorsal pallium of the telencephalon, numerous small pallial cells were ChAT‐like immunoreactive. In addition, tufted cells of the olfactory bulb and some cells in the lateral pallium showed faint immunoreactivity. In the preoptic‐hypothalamic region, ChAT‐immunoreactive (ChAT‐ir) cells were found in the preoptic nucleus, the vascular organ of the terminal lamina, and a small population in the caudal tuber. In the epithalamus, the pineal photoreceptors were intensely positive. Many cells of the habenula were faintly ChAT‐ir, but the neuropil of the interpeduncular nucleus showed intense ChAT immunoreactivity. In the pretectal region, ChAT‐ir cells were observed only in the superficial pretectal nucleus. In the brainstem, the somatomotor and branchiomotor nuclei, the octavolateral efferent nucleus, and a cell group just rostral to the Edinger‐Westphal (EW) nucleus contained ChAT‐ir neurons. In addition, the trigeminal mesencephalic nucleus, the nucleus G of the isthmus, some locus coeruleus cells, and some cell populations of the vestibular nuclei and of the electroreceptive nucleus of the octavolateral region exhibited ChAT immunoreactivity. In the reticular areas of the brainstem, the nucleus of the medial longitudinal fascicle, many reticular neurons of the rhombencephalon, and cells of the nucleus of the lateral funiculus were immunoreactive to this antibody. In the cerebellum, Golgi cells of the granule cell layer and some cells of the cerebellar nucleus were also ChAT‐ir. In the rostral spinal cord, ChAT immunoreactivity was observed in cells of the motor column, the dorsal horn, the marginal nucleus (a putative stretch‐receptor organ), and in interstitial cells of the ventral funiculus. These results demonstrate for the first time that cholinergic neurons are distributed widely in the central nervous system of elasmobranchs and that their cholinergic systems have evolved several characteristics that are unique to this group. J. Comp. Neurol. 420:139–170, 2000. © 2000 Wiley‐Liss, Inc.</abstract><qualityIndicators><score>8</score><pdfVersion>1.2</pdfVersion><pdfPageSize>594 x 792 pts</pdfPageSize><refBibsNative>true</refBibsNative><abstractCharCount>2800</abstractCharCount><pdfWordCount>18725</pdfWordCount><pdfCharCount>119333</pdfCharCount><pdfPageCount>32</pdfPageCount><abstractWordCount>400</abstractWordCount></qualityIndicators><title>Distribution of choline acetyltransferase immunoreactivity in the brain of an elasmobranch, the lesser spotted dogfish (Scyliorhinus canicula)</title><genre><json:string>article</json:string></genre><host><volume>420</volume><publisherId><json:string>CNE</json:string></publisherId><pages><total>32</total><last>170</last><first>139</first></pages><issn><json:string>0021-9967</json:string></issn><issue>2</issue><subject><json:item><value>Article</value></json:item></subject><genre><json:string>journal</json:string></genre><language><json:string>unknown</json:string></language><eissn><json:string>1096-9861</json:string></eissn><title>Journal of Comparative Neurology</title><doi><json:string>10.1002/(ISSN)1096-9861</json:string></doi></host><categories><wos><json:string>science</json:string><json:string>zoology</json:string><json:string>neurosciences</json:string></wos><scienceMetrix><json:string>health sciences</json:string><json:string>clinical medicine</json:string><json:string>neurology & neurosurgery</json:string></scienceMetrix></categories><publicationDate>2000</publicationDate><copyrightDate>2000</copyrightDate><doi><json:string>10.1002/(SICI)1096-9861(20000501)420:2>139::AID-CNE1>3.0.CO;2-T</json:string></doi><id>5E0DCCC0DB7DC2395E94A4966E236310AC63C739</id><score>0.01566074</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/document/5E0DCCC0DB7DC2395E94A4966E236310AC63C739/fulltext/pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/document/5E0DCCC0DB7DC2395E94A4966E236310AC63C739/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/5E0DCCC0DB7DC2395E94A4966E236310AC63C739/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Distribution of choline acetyltransferase immunoreactivity in the brain of an elasmobranch, the lesser spotted dogfish (Scyliorhinus canicula)</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>John Wiley & Sons, Inc.</publisher><pubPlace>New York</pubPlace><availability><p>Copyright © 2000 Wiley‐Liss, Inc.</p></availability><date>2000</date></publicationStmt><notesStmt><note>Spanish Education Ministry - No. PB96‐0945‐C03; No. PB96‐0606;</note><note>The Xunta de Galicia - No. XUGA20002B97;</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Distribution of choline acetyltransferase immunoreactivity in the brain of an elasmobranch, the lesser spotted dogfish (Scyliorhinus canicula)</title><author xml:id="author-1"><persName><forename type="first">Ramón</forename><surname>Anadón</surname></persName><affiliation>Department of Fundamental Biology, University of Santiago de Compostela, 15706‐Santiago de Compostela, Spain</affiliation><affiliation>Department of Fundamental Biology, University of Santiago de Compostela, 15706‐Santiago de Compostela, Spain</affiliation></author><author xml:id="author-2"><persName><forename type="first">Pilar</forename><surname>Molist</surname></persName><affiliation>Department of Functional Biology and Health Sciences, University of Vigo, 36200‐Vigo, Spain</affiliation></author><author xml:id="author-3"><persName><forename type="first">Isabel</forename><surname>Rodríguez‐Moldes</surname></persName><affiliation>Department of Fundamental Biology, University of Santiago de Compostela, 15706‐Santiago de Compostela, Spain</affiliation></author><author xml:id="author-4"><persName><forename type="first">Jesús María</forename><surname>López</surname></persName><affiliation>Department of Cell Biology, University Complutense of Madrid, 28040‐Madrid, Spain</affiliation></author><author xml:id="author-5"><persName><forename type="first">Inés</forename><surname>Quintela</surname></persName><affiliation>Department of Fundamental Biology, University of Santiago de Compostela, 15706‐Santiago de Compostela, Spain</affiliation><affiliation>Department of Biochemistry and Molecular Biology, University of Santiago de Compostela, 15706‐Santiago de Compostela, Spain</affiliation></author><author xml:id="author-6"><persName><forename type="first">María Carmen</forename><surname>Cerviño</surname></persName><affiliation>Department of Biochemistry and Molecular Biology, University of Santiago de Compostela, 15706‐Santiago de Compostela, Spain</affiliation></author><author xml:id="author-7"><persName><forename type="first">Primitivo</forename><surname>Barja</surname></persName><affiliation>Department of Biochemistry and Molecular Biology, University of Santiago de Compostela, 15706‐Santiago de Compostela, Spain</affiliation></author><author xml:id="author-8"><persName><forename type="first">Agustín</forename><surname>González</surname></persName><affiliation>Department of Cell Biology, University Complutense of Madrid, 28040‐Madrid, Spain</affiliation></author></analytic><monogr><title level="j">Journal of Comparative Neurology</title><title level="j" type="abbrev">J. Comp. Neurol.</title><idno type="pISSN">0021-9967</idno><idno type="eISSN">1096-9861</idno><idno type="DOI">10.1002/(ISSN)1096-9861</idno><imprint><publisher>John Wiley & Sons, Inc.</publisher><pubPlace>New York</pubPlace><date type="published" when="2000-05-01"></date><biblScope unit="volume">420</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="139">139</biblScope><biblScope unit="page" to="170">170</biblScope></imprint></monogr><idno type="istex">5E0DCCC0DB7DC2395E94A4966E236310AC63C739</idno><idno type="DOI">10.1002/(SICI)1096-9861(20000501)420:2<139::AID-CNE1>3.0.CO;2-T</idno><idno type="ArticleID">CNE1</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2000</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Although the distribution of cholinergic cells is remarkably similar across the vertebrate species, no data are available on more primitive species, such as cartilaginous fishes. To extend the evolutionary analysis of the cholinergic systems, we studied the distribution of cholinergic neurons in the brain and rostral spinal cord of Scyliorhinus canicula by immunocytochemistry using an antibody against the enzyme choline acetyltransferase (ChAT). Western blot analysis of brain extracts of dogfish, sturgeon, trout, and rat showed that this antibody recognized similar bands in the four species. Putative cholinergic neurons were observed in most brain regions, including the telencephalon, diencephalon, cerebellum, and brainstem. In the retrobulbar region and superficial dorsal pallium of the telencephalon, numerous small pallial cells were ChAT‐like immunoreactive. In addition, tufted cells of the olfactory bulb and some cells in the lateral pallium showed faint immunoreactivity. In the preoptic‐hypothalamic region, ChAT‐immunoreactive (ChAT‐ir) cells were found in the preoptic nucleus, the vascular organ of the terminal lamina, and a small population in the caudal tuber. In the epithalamus, the pineal photoreceptors were intensely positive. Many cells of the habenula were faintly ChAT‐ir, but the neuropil of the interpeduncular nucleus showed intense ChAT immunoreactivity. In the pretectal region, ChAT‐ir cells were observed only in the superficial pretectal nucleus. In the brainstem, the somatomotor and branchiomotor nuclei, the octavolateral efferent nucleus, and a cell group just rostral to the Edinger‐Westphal (EW) nucleus contained ChAT‐ir neurons. In addition, the trigeminal mesencephalic nucleus, the nucleus G of the isthmus, some locus coeruleus cells, and some cell populations of the vestibular nuclei and of the electroreceptive nucleus of the octavolateral region exhibited ChAT immunoreactivity. In the reticular areas of the brainstem, the nucleus of the medial longitudinal fascicle, many reticular neurons of the rhombencephalon, and cells of the nucleus of the lateral funiculus were immunoreactive to this antibody. In the cerebellum, Golgi cells of the granule cell layer and some cells of the cerebellar nucleus were also ChAT‐ir. In the rostral spinal cord, ChAT immunoreactivity was observed in cells of the motor column, the dorsal horn, the marginal nucleus (a putative stretch‐receptor organ), and in interstitial cells of the ventral funiculus. These results demonstrate for the first time that cholinergic neurons are distributed widely in the central nervous system of elasmobranchs and that their cholinergic systems have evolved several characteristics that are unique to this group. J. Comp. Neurol. 420:139–170, 2000. © 2000 Wiley‐Liss, Inc.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>keywords</head><item><term>acetylcholine</term></item><item><term>brain</term></item><item><term>immunohistochemistry</term></item><item><term>immunoblotting</term></item><item><term>dogfish</term></item><item><term>elasmobranchs</term></item></list></keywords></textClass><textClass><keywords scheme="Journal Subject"><list><head>article-category</head><item><term>Article</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1999-04-20">Received</change><change when="1999-12-15">Registration</change><change when="2000-05-01">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/5E0DCCC0DB7DC2395E94A4966E236310AC63C739/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>John Wiley & Sons, Inc.</publisherName><publisherLoc>New York</publisherLoc></publisherInfo><doi registered="yes">10.1002/(ISSN)1096-9861</doi><issn type="print">0021-9967</issn><issn type="electronic">1096-9861</issn><idGroup><id type="product" value="CNE"></id></idGroup><titleGroup><title type="main" xml:lang="en">Journal of Comparative Neurology</title><title type="short">J. Comp. Neurol.</title></titleGroup></publicationMeta><publicationMeta level="part" position="20"><doi origin="wiley" registered="yes">10.1002/(SICI)1096-9861(20000501)420:2<>1.0.CO;2-3</doi><numberingGroup><numbering type="journalVolume" number="420">420</numbering><numbering type="journalIssue">2</numbering></numberingGroup><coverDate startDate="2000-05-01">1 May 2000</coverDate></publicationMeta><publicationMeta level="unit" type="article" position="1" status="forIssue"><doi origin="wiley" registered="yes">10.1002/(SICI)1096-9861(20000501)420:2<139::AID-CNE1>3.0.CO;2-T</doi><idGroup><id type="unit" value="CNE1"></id></idGroup><countGroup><count type="pageTotal" number="32"></count></countGroup><titleGroup><title type="articleCategory">Article</title><title type="tocHeading1">ARTICLES</title></titleGroup><copyright ownership="publisher">Copyright © 2000 Wiley‐Liss, Inc.</copyright><eventGroup><event type="manuscriptReceived" date="1999-04-20"></event><event type="manuscriptRevised" date="1999-12-06"></event><event type="manuscriptAccepted" date="1999-12-15"></event><event type="firstOnline" date="2000-04-06"></event><event type="publishedOnlineFinalForm" date="2000-04-06"></event><event type="xmlConverted" agent="Converter:JWSART34_TO_WML3G version:2.3.5 mode:FullText source:FullText result:FullText" date="2010-04-09"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:3.8.8" date="2014-01-15"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-10-24"></event></eventGroup><numberingGroup><numbering type="pageFirst">139</numbering><numbering type="pageLast">170</numbering></numberingGroup><correspondenceTo>Department of Fundamental Biology, University of Santiago de Compostela, 15706‐Santiago de Compostela, Spain</correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:CNE.CNE1.pdf"></link></linkGroup></publicationMeta><contentMeta><countGroup><count type="figureTotal" number="11"></count><count type="tableTotal" number="0"></count><count type="referenceTotal" number="180"></count><count type="wordTotal" number="21028"></count></countGroup><titleGroup><title type="main" xml:lang="en">Distribution of choline acetyltransferase immunoreactivity in the brain of an elasmobranch, the lesser spotted dogfish (<i>Scyliorhinus canicula</i>)</title><title type="short" xml:lang="en">CHOLINERGIC NEURONS IN THE ELASMOBRANCH BRAIN</title></titleGroup><creators><creator xml:id="au1" creatorRole="author" affiliationRef="#af1" corresponding="yes"><personName><givenNames>Ramón</givenNames><familyName>Anadón</familyName></personName><contactDetails><email>bfanadon@usc.es</email></contactDetails></creator><creator xml:id="au2" creatorRole="author" affiliationRef="#af2"><personName><givenNames>Pilar</givenNames><familyName>Molist</familyName></personName></creator><creator xml:id="au3" creatorRole="author" affiliationRef="#af1"><personName><givenNames>Isabel</givenNames><familyName>Rodríguez‐Moldes</familyName></personName></creator><creator xml:id="au4" creatorRole="author" affiliationRef="#af3"><personName><givenNames>Jesús María</givenNames><familyName>López</familyName></personName></creator><creator xml:id="au5" creatorRole="author" affiliationRef="#af1 #af4"><personName><givenNames>Inés</givenNames><familyName>Quintela</familyName></personName></creator><creator xml:id="au6" creatorRole="author" affiliationRef="#af4"><personName><givenNames>María Carmen</givenNames><familyName>Cerviño</familyName></personName></creator><creator xml:id="au7" creatorRole="author" affiliationRef="#af4"><personName><givenNames>Primitivo</givenNames><familyName>Barja</familyName></personName></creator><creator xml:id="au8" creatorRole="author" affiliationRef="#af3"><personName><givenNames>Agustín</givenNames><familyName>González</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="af1" countryCode="ES" type="organization"><unparsedAffiliation>Department of Fundamental Biology, University of Santiago de Compostela, 15706‐Santiago de Compostela, Spain</unparsedAffiliation></affiliation><affiliation xml:id="af2" countryCode="ES" type="organization"><unparsedAffiliation>Department of Functional Biology and Health Sciences, University of Vigo, 36200‐Vigo, Spain</unparsedAffiliation></affiliation><affiliation xml:id="af3" countryCode="ES" type="organization"><unparsedAffiliation>Department of Cell Biology, University Complutense of Madrid, 28040‐Madrid, Spain</unparsedAffiliation></affiliation><affiliation xml:id="af4" countryCode="ES" type="organization"><unparsedAffiliation>Department of Biochemistry and Molecular Biology, University of Santiago de Compostela, 15706‐Santiago de Compostela, Spain</unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en" type="author"><keyword xml:id="kwd1">acetylcholine</keyword><keyword xml:id="kwd2">brain</keyword><keyword xml:id="kwd3">immunohistochemistry</keyword><keyword xml:id="kwd4">immunoblotting</keyword><keyword xml:id="kwd5">dogfish</keyword><keyword xml:id="kwd6">elasmobranchs</keyword></keywordGroup><fundingInfo><fundingAgency>Spanish Education Ministry</fundingAgency><fundingNumber>PB96‐0945‐C03</fundingNumber><fundingNumber>PB96‐0606</fundingNumber></fundingInfo><fundingInfo><fundingAgency>The Xunta de Galicia</fundingAgency><fundingNumber>XUGA20002B97</fundingNumber></fundingInfo><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Abstract</title><p>Although the distribution of cholinergic cells is remarkably similar across the vertebrate species, no data are available on more primitive species, such as cartilaginous fishes. To extend the evolutionary analysis of the cholinergic systems, we studied the distribution of cholinergic neurons in the brain and rostral spinal cord of <i>Scyliorhinus canicula</i> by immunocytochemistry using an antibody against the enzyme choline acetyltransferase (ChAT). Western blot analysis of brain extracts of dogfish, sturgeon, trout, and rat showed that this antibody recognized similar bands in the four species. Putative cholinergic neurons were observed in most brain regions, including the telencephalon, diencephalon, cerebellum, and brainstem. In the retrobulbar region and superficial dorsal pallium of the telencephalon, numerous small pallial cells were ChAT‐like immunoreactive. In addition, tufted cells of the olfactory bulb and some cells in the lateral pallium showed faint immunoreactivity. In the preoptic‐hypothalamic region, ChAT‐immunoreactive (ChAT‐ir) cells were found in the preoptic nucleus, the vascular organ of the terminal lamina, and a small population in the caudal tuber. In the epithalamus, the pineal photoreceptors were intensely positive. Many cells of the habenula were faintly ChAT‐ir, but the neuropil of the interpeduncular nucleus showed intense ChAT immunoreactivity. In the pretectal region, ChAT‐ir cells were observed only in the superficial pretectal nucleus. In the brainstem, the somatomotor and branchiomotor nuclei, the octavolateral efferent nucleus, and a cell group just rostral to the Edinger‐Westphal (EW) nucleus contained ChAT‐ir neurons. In addition, the trigeminal mesencephalic nucleus, the nucleus G of the isthmus, some locus coeruleus cells, and some cell populations of the vestibular nuclei and of the electroreceptive nucleus of the octavolateral region exhibited ChAT immunoreactivity. In the reticular areas of the brainstem, the nucleus of the medial longitudinal fascicle, many reticular neurons of the rhombencephalon, and cells of the nucleus of the lateral funiculus were immunoreactive to this antibody. In the cerebellum, Golgi cells of the granule cell layer and some cells of the cerebellar nucleus were also ChAT‐ir. In the rostral spinal cord, ChAT immunoreactivity was observed in cells of the motor column, the dorsal horn, the marginal nucleus (a putative stretch‐receptor organ), and in interstitial cells of the ventral funiculus. These results demonstrate for the first time that cholinergic neurons are distributed widely in the central nervous system of elasmobranchs and that their cholinergic systems have evolved several characteristics that are unique to this group. J. Comp. Neurol. 420:139–170, 2000. © 2000 Wiley‐Liss, Inc.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Distribution of choline acetyltransferase immunoreactivity in the brain of an elasmobranch, the lesser spotted dogfish (Scyliorhinus canicula)</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>CHOLINERGIC NEURONS IN THE ELASMOBRANCH BRAIN</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Distribution of choline acetyltransferase immunoreactivity in the brain of an elasmobranch, the lesser spotted dogfish (Scyliorhinus canicula)</title></titleInfo><name type="personal"><namePart type="given">Ramón</namePart><namePart type="family">Anadón</namePart><affiliation>Department of Fundamental Biology, University of Santiago de Compostela, 15706‐Santiago de Compostela, Spain</affiliation><affiliation>Department of Fundamental Biology, University of Santiago de Compostela, 15706‐Santiago de Compostela, Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Pilar</namePart><namePart type="family">Molist</namePart><affiliation>Department of Functional Biology and Health Sciences, University of Vigo, 36200‐Vigo, Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Isabel</namePart><namePart type="family">Rodríguez‐Moldes</namePart><affiliation>Department of Fundamental Biology, University of Santiago de Compostela, 15706‐Santiago de Compostela, Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Jesús María</namePart><namePart type="family">López</namePart><affiliation>Department of Cell Biology, University Complutense of Madrid, 28040‐Madrid, Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Inés</namePart><namePart type="family">Quintela</namePart><affiliation>Department of Fundamental Biology, University of Santiago de Compostela, 15706‐Santiago de Compostela, Spain</affiliation><affiliation>Department of Biochemistry and Molecular Biology, University of Santiago de Compostela, 15706‐Santiago de Compostela, Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">María Carmen</namePart><namePart type="family">Cerviño</namePart><affiliation>Department of Biochemistry and Molecular Biology, University of Santiago de Compostela, 15706‐Santiago de Compostela, Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Primitivo</namePart><namePart type="family">Barja</namePart><affiliation>Department of Biochemistry and Molecular Biology, University of Santiago de Compostela, 15706‐Santiago de Compostela, Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Agustín</namePart><namePart type="family">González</namePart><affiliation>Department of Cell Biology, University Complutense of Madrid, 28040‐Madrid, Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>John Wiley & Sons, Inc.</publisher><place><placeTerm type="text">New York</placeTerm></place><dateIssued encoding="w3cdtf">2000-05-01</dateIssued><dateCaptured encoding="w3cdtf">1999-04-20</dateCaptured><dateValid encoding="w3cdtf">1999-12-15</dateValid><copyrightDate encoding="w3cdtf">2000</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">11</extent><extent unit="references">180</extent><extent unit="words">21028</extent></physicalDescription><abstract lang="en">Although the distribution of cholinergic cells is remarkably similar across the vertebrate species, no data are available on more primitive species, such as cartilaginous fishes. To extend the evolutionary analysis of the cholinergic systems, we studied the distribution of cholinergic neurons in the brain and rostral spinal cord of Scyliorhinus canicula by immunocytochemistry using an antibody against the enzyme choline acetyltransferase (ChAT). Western blot analysis of brain extracts of dogfish, sturgeon, trout, and rat showed that this antibody recognized similar bands in the four species. Putative cholinergic neurons were observed in most brain regions, including the telencephalon, diencephalon, cerebellum, and brainstem. In the retrobulbar region and superficial dorsal pallium of the telencephalon, numerous small pallial cells were ChAT‐like immunoreactive. In addition, tufted cells of the olfactory bulb and some cells in the lateral pallium showed faint immunoreactivity. In the preoptic‐hypothalamic region, ChAT‐immunoreactive (ChAT‐ir) cells were found in the preoptic nucleus, the vascular organ of the terminal lamina, and a small population in the caudal tuber. In the epithalamus, the pineal photoreceptors were intensely positive. Many cells of the habenula were faintly ChAT‐ir, but the neuropil of the interpeduncular nucleus showed intense ChAT immunoreactivity. In the pretectal region, ChAT‐ir cells were observed only in the superficial pretectal nucleus. In the brainstem, the somatomotor and branchiomotor nuclei, the octavolateral efferent nucleus, and a cell group just rostral to the Edinger‐Westphal (EW) nucleus contained ChAT‐ir neurons. In addition, the trigeminal mesencephalic nucleus, the nucleus G of the isthmus, some locus coeruleus cells, and some cell populations of the vestibular nuclei and of the electroreceptive nucleus of the octavolateral region exhibited ChAT immunoreactivity. In the reticular areas of the brainstem, the nucleus of the medial longitudinal fascicle, many reticular neurons of the rhombencephalon, and cells of the nucleus of the lateral funiculus were immunoreactive to this antibody. In the cerebellum, Golgi cells of the granule cell layer and some cells of the cerebellar nucleus were also ChAT‐ir. In the rostral spinal cord, ChAT immunoreactivity was observed in cells of the motor column, the dorsal horn, the marginal nucleus (a putative stretch‐receptor organ), and in interstitial cells of the ventral funiculus. These results demonstrate for the first time that cholinergic neurons are distributed widely in the central nervous system of elasmobranchs and that their cholinergic systems have evolved several characteristics that are unique to this group. J. Comp. Neurol. 420:139–170, 2000. © 2000 Wiley‐Liss, Inc.</abstract><note type="funding">Spanish Education Ministry - No. PB96‐0945‐C03; No. PB96‐0606; </note><note type="funding">The Xunta de Galicia - No. XUGA20002B97; </note><subject lang="en"><genre>keywords</genre><topic>acetylcholine</topic><topic>brain</topic><topic>immunohistochemistry</topic><topic>immunoblotting</topic><topic>dogfish</topic><topic>elasmobranchs</topic></subject><relatedItem type="host"><titleInfo><title>Journal of Comparative Neurology</title></titleInfo><titleInfo type="abbreviated"><title>J. Comp. Neurol.</title></titleInfo><genre type="journal">journal</genre><subject><genre>article-category</genre><topic>Article</topic></subject><identifier type="ISSN">0021-9967</identifier><identifier type="eISSN">1096-9861</identifier><identifier type="DOI">10.1002/(ISSN)1096-9861</identifier><identifier type="PublisherID">CNE</identifier><part><date>2000</date><detail type="volume"><caption>vol.</caption><number>420</number></detail><detail type="issue"><caption>no.</caption><number>2</number></detail><extent unit="pages"><start>139</start><end>170</end><total>32</total></extent></part></relatedItem><identifier type="istex">5E0DCCC0DB7DC2395E94A4966E236310AC63C739</identifier><identifier type="DOI">10.1002/(SICI)1096-9861(20000501)420:2<139::AID-CNE1>3.0.CO;2-T</identifier><identifier type="ArticleID">CNE1</identifier><accessCondition type="use and reproduction" contentType="copyright">Copyright © 2000 Wiley‐Liss, Inc.</accessCondition><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>John Wiley & Sons, Inc.</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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