Characterization of the cDNA encoding a somatostatin variant in the chicken brain: Comparison of the distribution of the two somatostatin precursor mRNAs
Identifieur interne : 001324 ( Istex/Corpus ); précédent : 001323; suivant : 001325Characterization of the cDNA encoding a somatostatin variant in the chicken brain: Comparison of the distribution of the two somatostatin precursor mRNAs
Auteurs : Michele Trabucchi ; Hervé Tostivint ; Isabelle Lihrmann ; Sabine Bl Hser ; Mauro Vallarino ; Hubert VaudrySource :
- Journal of Comparative Neurology [ 0021-9967 ] ; 2003-07-07.
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Abstract
Although the existence of two somatostatin variants (SS1 and SS2) has now been demonstrated in the brain of mammals, amphibians, and fish, only one isoform of somatostatin (SS1) has been characterized to date in the brain of birds. Here we report cloning of the cDNA encoding a 101‐amino‐acid protein (PSS2) that encompasses the somatostatin variant [Pro2]somatostatin‐14 (SS2) at its C‐terminus. Sequence analysis indicated that chicken PSS2 is more closely related to fish PSS2 than to mammalian cortistatin precursors. Northern blot analysis showed that the chicken PSS1 gene is expressed in the central nervous system (CNS) and in the pancreas, whereas the PSS2 gene is expressed only in the CNS and not in peripheral organs. In situ hybridization histochemistry revealed that, in the chicken brain, PSS1 mRNA is more widely distributed than PSS2 mRNA. In particular, PSS1 mRNA expression was found in the hippocampus, the hyperstriatum, the preoptic area, the ventricular hypothalamic nuclei, the optic tectum, and several nuclei of the mesencephalon and rhombencephalon. In contrast, the distribution of PSS2 mRNA was restricted to a few regions of the brain, including the paraolfactory lobe, the paleostriatum, and some nuclei of the mesencephalon and rhombencephalon. The fact that the PSS1 and PSS2 genes are differently expressed in the brain and in peripheral organs indicates that, in chicken, the two somatostatin variants likely exert distinct functions. In particular, the observation that PSS1 mRNA, but not PSS2 mRNA, occurs in the preoptic area and in the ventral hypothalamic nuclei suggests that, of the two somatostatin isoforms, only SS1 acts as a hypophysiotropic factor. J. Comp. Neurol. 461:441–451, 2003. © 2003 Wiley‐Liss, Inc.
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DOI: 10.1002/cne.10690
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Here we report cloning of the cDNA encoding a 101‐amino‐acid protein (PSS2) that encompasses the somatostatin variant [Pro2]somatostatin‐14 (SS2) at its C‐terminus. Sequence analysis indicated that chicken PSS2 is more closely related to fish PSS2 than to mammalian cortistatin precursors. Northern blot analysis showed that the chicken PSS1 gene is expressed in the central nervous system (CNS) and in the pancreas, whereas the PSS2 gene is expressed only in the CNS and not in peripheral organs. In situ hybridization histochemistry revealed that, in the chicken brain, PSS1 mRNA is more widely distributed than PSS2 mRNA. In particular, PSS1 mRNA expression was found in the hippocampus, the hyperstriatum, the preoptic area, the ventricular hypothalamic nuclei, the optic tectum, and several nuclei of the mesencephalon and rhombencephalon. 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Here we report cloning of the cDNA encoding a 101‐amino‐acid protein (PSS2) that encompasses the somatostatin variant [Pro2]somatostatin‐14 (SS2) at its C‐terminus. Sequence analysis indicated that chicken PSS2 is more closely related to fish PSS2 than to mammalian cortistatin precursors. Northern blot analysis showed that the chicken PSS1 gene is expressed in the central nervous system (CNS) and in the pancreas, whereas the PSS2 gene is expressed only in the CNS and not in peripheral organs. In situ hybridization histochemistry revealed that, in the chicken brain, PSS1 mRNA is more widely distributed than PSS2 mRNA. In particular, PSS1 mRNA expression was found in the hippocampus, the hyperstriatum, the preoptic area, the ventricular hypothalamic nuclei, the optic tectum, and several nuclei of the mesencephalon and rhombencephalon. In contrast, the distribution of PSS2 mRNA was restricted to a few regions of the brain, including the paraolfactory lobe, the paleostriatum, and some nuclei of the mesencephalon and rhombencephalon. The fact that the PSS1 and PSS2 genes are differently expressed in the brain and in peripheral organs indicates that, in chicken, the two somatostatin variants likely exert distinct functions. In particular, the observation that PSS1 mRNA, but not PSS2 mRNA, occurs in the preoptic area and in the ventral hypothalamic nuclei suggests that, of the two somatostatin isoforms, only SS1 acts as a hypophysiotropic factor. J. Comp. 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No. U‐413;</note><note>MURST (40%)</note><note>Institut National de la Santé et de la Recherche Médicale and Centro Nazionale delle Ricerche (INSERM/CNR Exchange Program)</note><note>Conseil Régional de Haute‐Normandie</note><note>National Science Foundation - No. IBN‐9810516;</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Characterization of the cDNA encoding a somatostatin variant in the chicken brain: Comparison of the distribution of the two somatostatin precursor mRNAs</title><author xml:id="author-1"><persName><forename type="first">Michele</forename><surname>Trabucchi</surname></persName><affiliation>European Institute for Peptide Research (IFRMP 23), Laboratory of Cellular and Molecular Neuroendocrinology, INSERM U413, UA CNRS, University of Rouen, 76821 Mont‐Saint‐Aignan, France</affiliation><affiliation>Department of Experimental Biology, DIBISAA, University of Genova, 16132 Genova, Italy</affiliation></author><author xml:id="author-2"><persName><forename type="first">Hervé</forename><surname>Tostivint</surname></persName><affiliation>European Institute for Peptide Research (IFRMP 23), Laboratory of Cellular and Molecular Neuroendocrinology, INSERM U413, UA CNRS, University of Rouen, 76821 Mont‐Saint‐Aignan, France</affiliation></author><author xml:id="author-3"><persName><forename type="first">Isabelle</forename><surname>Lihrmann</surname></persName><affiliation>European Institute for Peptide Research (IFRMP 23), Laboratory of Cellular and Molecular Neuroendocrinology, INSERM U413, UA CNRS, University of Rouen, 76821 Mont‐Saint‐Aignan, France</affiliation></author><author xml:id="author-4"><persName><forename type="first">Sabine</forename><surname>Blähser</surname></persName><affiliation>Institute of Anatomy‐Cytobiology, University of Giessen, 35392 Giessen, Germany</affiliation></author><author xml:id="author-5"><persName><forename type="first">Mauro</forename><surname>Vallarino</surname></persName><affiliation>Department of Experimental Biology, DIBISAA, University of Genova, 16132 Genova, Italy</affiliation></author><author xml:id="author-6"><persName><forename type="first">Hubert</forename><surname>Vaudry</surname></persName><affiliation>European Institute for Peptide Research (IFRMP 23), Laboratory of Cellular and Molecular Neuroendocrinology, INSERM U413, UA CNRS, University of Rouen, 76821 Mont‐Saint‐Aignan, France</affiliation><affiliation>European Institute for Peptide Research (IFRMP 23), Laboratory of Cellular and Molecular Neuroendocrinology, INSERM U‐413, UA CNRS, University of Rouen, 76821 Mont‐Saint‐Aignan, France</affiliation></author></analytic><monogr><title level="j">Journal of Comparative Neurology</title><title level="j" type="abbrev">J. 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Here we report cloning of the cDNA encoding a 101‐amino‐acid protein (PSS2) that encompasses the somatostatin variant [Pro2]somatostatin‐14 (SS2) at its C‐terminus. Sequence analysis indicated that chicken PSS2 is more closely related to fish PSS2 than to mammalian cortistatin precursors. Northern blot analysis showed that the chicken PSS1 gene is expressed in the central nervous system (CNS) and in the pancreas, whereas the PSS2 gene is expressed only in the CNS and not in peripheral organs. In situ hybridization histochemistry revealed that, in the chicken brain, PSS1 mRNA is more widely distributed than PSS2 mRNA. In particular, PSS1 mRNA expression was found in the hippocampus, the hyperstriatum, the preoptic area, the ventricular hypothalamic nuclei, the optic tectum, and several nuclei of the mesencephalon and rhombencephalon. In contrast, the distribution of PSS2 mRNA was restricted to a few regions of the brain, including the paraolfactory lobe, the paleostriatum, and some nuclei of the mesencephalon and rhombencephalon. The fact that the PSS1 and PSS2 genes are differently expressed in the brain and in peripheral organs indicates that, in chicken, the two somatostatin variants likely exert distinct functions. In particular, the observation that PSS1 mRNA, but not PSS2 mRNA, occurs in the preoptic area and in the ventral hypothalamic nuclei suggests that, of the two somatostatin isoforms, only SS1 acts as a hypophysiotropic factor. J. Comp. 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href="file:CNE.CNE10690.pdf"></link></linkGroup></publicationMeta><contentMeta><countGroup><count type="figureTotal" number="6"></count><count type="tableTotal" number="1"></count><count type="referenceTotal" number="53"></count><count type="wordTotal" number="7755"></count></countGroup><titleGroup><title type="main" xml:lang="en">Characterization of the cDNA encoding a somatostatin variant in the chicken brain: Comparison of the distribution of the two somatostatin precursor mRNAs</title><title type="short" xml:lang="en">Two Somatostatin Variants in Chicken Brain</title></titleGroup><creators><creator xml:id="au1" creatorRole="author" affiliationRef="#af1 #af2"><personName><givenNames>Michele</givenNames><familyName>Trabucchi</familyName></personName></creator><creator xml:id="au2" creatorRole="author" affiliationRef="#af1"><personName><givenNames>Hervé</givenNames><familyName>Tostivint</familyName></personName></creator><creator xml:id="au3" creatorRole="author" affiliationRef="#af1"><personName><givenNames>Isabelle</givenNames><familyName>Lihrmann</familyName></personName></creator><creator xml:id="au4" creatorRole="author" affiliationRef="#af3"><personName><givenNames>Sabine</givenNames><familyName>Blähser</familyName></personName></creator><creator xml:id="au5" creatorRole="author" affiliationRef="#af2"><personName><givenNames>Mauro</givenNames><familyName>Vallarino</familyName></personName></creator><creator xml:id="au6" creatorRole="author" affiliationRef="#af1" corresponding="yes"><personName><givenNames>Hubert</givenNames><familyName>Vaudry</familyName></personName><contactDetails><email normalForm="hubert.vaudry@univ-rouen.fr">hubert.vaudry@univ‐rouen.fr</email></contactDetails></creator></creators><affiliationGroup><affiliation xml:id="af1" countryCode="FR" type="organization"><unparsedAffiliation>European Institute for Peptide Research (IFRMP 23), Laboratory of Cellular and Molecular Neuroendocrinology, INSERM U413, UA CNRS, University of Rouen, 76821 Mont‐Saint‐Aignan, France</unparsedAffiliation></affiliation><affiliation xml:id="af2" countryCode="IT" type="organization"><unparsedAffiliation>Department of Experimental Biology, DIBISAA, University of Genova, 16132 Genova, Italy</unparsedAffiliation></affiliation><affiliation xml:id="af3" countryCode="DE" type="organization"><unparsedAffiliation>Institute of Anatomy‐Cytobiology, University of Giessen, 35392 Giessen, Germany</unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en" type="author"><keyword xml:id="kwd1">SRIF</keyword><keyword xml:id="kwd2">cortistatin</keyword><keyword xml:id="kwd3">central nervous system</keyword><keyword xml:id="kwd4">birds</keyword><keyword xml:id="kwd5">evolution</keyword></keywordGroup><fundingInfo><fundingAgency>Institut National de la Santé et de la Recherche Médicale (INSERM)</fundingAgency><fundingNumber>U‐413</fundingNumber></fundingInfo><fundingInfo><fundingAgency>MURST (40%)</fundingAgency></fundingInfo><fundingInfo><fundingAgency>Institut National de la Santé et de la Recherche Médicale and Centro Nazionale delle Ricerche (INSERM/CNR Exchange Program)</fundingAgency></fundingInfo><fundingInfo><fundingAgency>Conseil Régional de Haute‐Normandie</fundingAgency></fundingInfo><fundingInfo><fundingAgency>National Science Foundation</fundingAgency><fundingNumber>IBN‐9810516</fundingNumber></fundingInfo><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Abstract</title><p>Although the existence of two somatostatin variants (SS1 and SS2) has now been demonstrated in the brain of mammals, amphibians, and fish, only one isoform of somatostatin (SS1) has been characterized to date in the brain of birds. Here we report cloning of the cDNA encoding a 101‐amino‐acid protein (PSS2) that encompasses the somatostatin variant [Pro<sup>2</sup>]somatostatin‐14 (SS2) at its C‐terminus. Sequence analysis indicated that chicken PSS2 is more closely related to fish PSS2 than to mammalian cortistatin precursors. Northern blot analysis showed that the chicken PSS1 gene is expressed in the central nervous system (CNS) and in the pancreas, whereas the PSS2 gene is expressed only in the CNS and not in peripheral organs. In situ hybridization histochemistry revealed that, in the chicken brain, PSS1 mRNA is more widely distributed than PSS2 mRNA. In particular, PSS1 mRNA expression was found in the hippocampus, the hyperstriatum, the preoptic area, the ventricular hypothalamic nuclei, the optic tectum, and several nuclei of the mesencephalon and rhombencephalon. In contrast, the distribution of PSS2 mRNA was restricted to a few regions of the brain, including the paraolfactory lobe, the paleostriatum, and some nuclei of the mesencephalon and rhombencephalon. The fact that the PSS1 and PSS2 genes are differently expressed in the brain and in peripheral organs indicates that, in chicken, the two somatostatin variants likely exert distinct functions. In particular, the observation that PSS1 mRNA, but not PSS2 mRNA, occurs in the preoptic area and in the ventral hypothalamic nuclei suggests that, of the two somatostatin isoforms, only SS1 acts as a hypophysiotropic factor. J. Comp. Neurol. 461:441–451, 2003. © 2003 Wiley‐Liss, Inc.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Characterization of the cDNA encoding a somatostatin variant in the chicken brain: Comparison of the distribution of the two somatostatin precursor mRNAs</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>Two Somatostatin Variants in Chicken Brain</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Characterization of the cDNA encoding a somatostatin variant in the chicken brain: Comparison of the distribution of the two somatostatin precursor mRNAs</title></titleInfo><name type="personal"><namePart type="given">Michele</namePart><namePart type="family">Trabucchi</namePart><affiliation>European Institute for Peptide Research (IFRMP 23), Laboratory of Cellular and Molecular Neuroendocrinology, INSERM U413, UA CNRS, University of Rouen, 76821 Mont‐Saint‐Aignan, France</affiliation><affiliation>Department of Experimental Biology, DIBISAA, University of Genova, 16132 Genova, Italy</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Hervé</namePart><namePart type="family">Tostivint</namePart><affiliation>European Institute for Peptide Research (IFRMP 23), Laboratory of Cellular and Molecular Neuroendocrinology, INSERM U413, UA CNRS, University of Rouen, 76821 Mont‐Saint‐Aignan, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Isabelle</namePart><namePart type="family">Lihrmann</namePart><affiliation>European Institute for Peptide Research (IFRMP 23), Laboratory of Cellular and Molecular Neuroendocrinology, INSERM U413, UA CNRS, University of Rouen, 76821 Mont‐Saint‐Aignan, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Sabine</namePart><namePart type="family">Blähser</namePart><affiliation>Institute of Anatomy‐Cytobiology, University of Giessen, 35392 Giessen, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Mauro</namePart><namePart type="family">Vallarino</namePart><affiliation>Department of Experimental Biology, DIBISAA, University of Genova, 16132 Genova, Italy</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Hubert</namePart><namePart type="family">Vaudry</namePart><affiliation>European Institute for Peptide Research (IFRMP 23), Laboratory of Cellular and Molecular Neuroendocrinology, INSERM U413, UA CNRS, University of Rouen, 76821 Mont‐Saint‐Aignan, France</affiliation><affiliation>European Institute for Peptide Research (IFRMP 23), Laboratory of Cellular and Molecular Neuroendocrinology, INSERM U‐413, UA CNRS, University of Rouen, 76821 Mont‐Saint‐Aignan, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><place><placeTerm type="text">Hoboken</placeTerm></place><dateIssued encoding="w3cdtf">2003-07-07</dateIssued><dateCaptured encoding="w3cdtf">2002-07-16</dateCaptured><dateValid encoding="w3cdtf">2003-02-06</dateValid><copyrightDate encoding="w3cdtf">2003</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">6</extent><extent unit="tables">1</extent><extent unit="references">53</extent><extent unit="words">7755</extent></physicalDescription><abstract lang="en">Although the existence of two somatostatin variants (SS1 and SS2) has now been demonstrated in the brain of mammals, amphibians, and fish, only one isoform of somatostatin (SS1) has been characterized to date in the brain of birds. Here we report cloning of the cDNA encoding a 101‐amino‐acid protein (PSS2) that encompasses the somatostatin variant [Pro2]somatostatin‐14 (SS2) at its C‐terminus. Sequence analysis indicated that chicken PSS2 is more closely related to fish PSS2 than to mammalian cortistatin precursors. Northern blot analysis showed that the chicken PSS1 gene is expressed in the central nervous system (CNS) and in the pancreas, whereas the PSS2 gene is expressed only in the CNS and not in peripheral organs. In situ hybridization histochemistry revealed that, in the chicken brain, PSS1 mRNA is more widely distributed than PSS2 mRNA. In particular, PSS1 mRNA expression was found in the hippocampus, the hyperstriatum, the preoptic area, the ventricular hypothalamic nuclei, the optic tectum, and several nuclei of the mesencephalon and rhombencephalon. In contrast, the distribution of PSS2 mRNA was restricted to a few regions of the brain, including the paraolfactory lobe, the paleostriatum, and some nuclei of the mesencephalon and rhombencephalon. The fact that the PSS1 and PSS2 genes are differently expressed in the brain and in peripheral organs indicates that, in chicken, the two somatostatin variants likely exert distinct functions. In particular, the observation that PSS1 mRNA, but not PSS2 mRNA, occurs in the preoptic area and in the ventral hypothalamic nuclei suggests that, of the two somatostatin isoforms, only SS1 acts as a hypophysiotropic factor. J. Comp. Neurol. 461:441–451, 2003. © 2003 Wiley‐Liss, Inc.</abstract><note type="funding">Institut National de la Santé et de la Recherche Médicale (INSERM) - No. U‐413; </note><note type="funding">MURST (40%)</note><note type="funding">Institut National de la Santé et de la Recherche Médicale and Centro Nazionale delle Ricerche (INSERM/CNR Exchange Program)</note><note type="funding">Conseil Régional de Haute‐Normandie</note><note type="funding">National Science Foundation - No. IBN‐9810516; </note><subject lang="en"><genre>keywords</genre><topic>SRIF</topic><topic>cortistatin</topic><topic>central nervous system</topic><topic>birds</topic><topic>evolution</topic></subject><relatedItem type="host"><titleInfo><title>Journal of Comparative Neurology</title></titleInfo><titleInfo type="abbreviated"><title>J. Comp. Neurol.</title></titleInfo><genre type="journal">journal</genre><subject><genre>article-category</genre><topic>Article</topic></subject><identifier type="ISSN">0021-9967</identifier><identifier type="eISSN">1096-9861</identifier><identifier type="DOI">10.1002/(ISSN)1096-9861</identifier><identifier type="PublisherID">CNE</identifier><part><date>2003</date><detail type="volume"><caption>vol.</caption><number>461</number></detail><detail type="issue"><caption>no.</caption><number>4</number></detail><extent unit="pages"><start>441</start><end>451</end><total>11</total></extent></part></relatedItem><identifier type="istex">167E43F99FE00BE2179CDF3FBE4FB83144C051B5</identifier><identifier type="DOI">10.1002/cne.10690</identifier><identifier type="ArticleID">CNE10690</identifier><accessCondition type="use and reproduction" contentType="copyright">Copyright © 2003 Wiley‐Liss, Inc.</accessCondition><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>Wiley Subscription Services, Inc., A Wiley Company</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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