Spawning induces a shift in energy metabolism from glucose to lipid in rainbow trout white muscle
Identifieur interne : 001304 ( Istex/Corpus ); précédent : 001303; suivant : 001305Spawning induces a shift in energy metabolism from glucose to lipid in rainbow trout white muscle
Auteurs : A. Kiessling ; L. Larsson ; K. H. Kiessling ; P. B. Lutes ; T. Storebakken ; S. S. S. HungSource :
- Fish Physiology and Biochemistry [ 0920-1742 ] ; 1995-12-01.
Abstract
Abstract: Enzymatic changes that occur in the white somatic muscle of rainbow trout (Oncorhynchus mykiss) in response to spawning were investigated, and the evenness of their distribution across the ventral-dorsal plane of this muscle was assessed. Four enzymes that are involved in energy metabolism were measured (phosphofructokinase: glycolytic capacity, 3-hydroxyacyl-CoA dehydrogenase: β-oxidation, citrate synthase: citric acid cycle, cytochrome oxidase: oxidative capacity). The enzyme activities were followed in different parts of the white muscle of non-spawning female rainbow trout from May, four months after their first spawning, until December, at second spawning. Samples were taken from white epaxial muscle along the lateral line, on the dorsum, and in between. Samples were also taken from red muscle of non-spawning fish. The isoforms of myosin heavy chains (MyHC) were electrophoretically identified on 6% SDS-PAGE gel to study possible changes in contractile properties of the muscle. Transformation from the non-spawning to spawning phase was associated with dramatic changes in the activity of the enzymes studied in white muscle: glycolytic capacity decreased to less than half, whereas oxidative metabolism increased about two- to four-fold in all areas. Significant quantitative differences in enzyme activities were found between the three epaxial muscle areas: in the non-spawning fish lateral line samples differed from those taken in the other two areas, whereas in spawning fish the dorsal sample difered from the other two. No difference in the expression of MyHC-isoforms was found between spawning and non-spawning fish. Co-expression of both slow and fast isoforms was found in single fibres isolated from red muscle. The results show that the energy metabolism in white muscle of domestic rainbow trout is altered during spawning; i.e., the metabolism becomes increasingly aerobic, with an increased capacity for fatty acid utilization, concomitant with phenotypic changes associated with sexual maturation. These changes are especially pronounced in ventral, superficially located fibres.
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DOI: 10.1007/BF00004344
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ISTEX:57F1F83A05B590E77CAE11A60668D1675EC72B38Le document en format XML
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H." last="Kiessling">K. H. Kiessling</name><affiliation><mods:affiliation>Animal Husbandry, University of Agricultural Sciences, Uppsala, Sweden</mods:affiliation></affiliation></author><author><name sortKey="Lutes, P B" sort="Lutes, P B" uniqKey="Lutes P" first="P. B." last="Lutes">P. B. Lutes</name><affiliation><mods:affiliation>Department of Animal Science, University of California, Davis, U.S.A.</mods:affiliation></affiliation></author><author><name sortKey="Storebakken, T" sort="Storebakken, T" uniqKey="Storebakken T" first="T." last="Storebakken">T. Storebakken</name><affiliation><mods:affiliation>AKVAFORSK Ltd, Sunndlöra, Norway</mods:affiliation></affiliation></author><author><name sortKey="Hung, S S S" sort="Hung, S S S" uniqKey="Hung S" first="S. S. S." last="Hung">S. S. S. Hung</name><affiliation><mods:affiliation>Department of Animal Science, University of California, Davis, U.S.A.</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:57F1F83A05B590E77CAE11A60668D1675EC72B38</idno><date when="1995" year="1995">1995</date><idno type="doi">10.1007/BF00004344</idno><idno type="url">https://api.istex.fr/document/57F1F83A05B590E77CAE11A60668D1675EC72B38/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">001304</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001304</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Spawning induces a shift in energy metabolism from glucose to lipid in rainbow trout white muscle</title><author><name sortKey="Kiessling, A" sort="Kiessling, A" uniqKey="Kiessling A" first="A." last="Kiessling">A. Kiessling</name><affiliation><mods:affiliation>Departments of Aquaculture, University of Agricultural Sciences, Uppsala, Sweden</mods:affiliation></affiliation><affiliation><mods:affiliation>Animal Husbandry, University of Agricultural Sciences, Uppsala, Sweden</mods:affiliation></affiliation></author><author><name sortKey="Larsson, L" sort="Larsson, L" uniqKey="Larsson L" first="L." last="Larsson">L. Larsson</name><affiliation><mods:affiliation>Department of Clinical Neurophysiology, Karolinska Hospital, Stockholm, Sweden</mods:affiliation></affiliation></author><author><name sortKey="Kiessling, K H" sort="Kiessling, K H" uniqKey="Kiessling K" first="K. H." last="Kiessling">K. H. Kiessling</name><affiliation><mods:affiliation>Animal Husbandry, University of Agricultural Sciences, Uppsala, Sweden</mods:affiliation></affiliation></author><author><name sortKey="Lutes, P B" sort="Lutes, P B" uniqKey="Lutes P" first="P. B." last="Lutes">P. B. Lutes</name><affiliation><mods:affiliation>Department of Animal Science, University of California, Davis, U.S.A.</mods:affiliation></affiliation></author><author><name sortKey="Storebakken, T" sort="Storebakken, T" uniqKey="Storebakken T" first="T." last="Storebakken">T. Storebakken</name><affiliation><mods:affiliation>AKVAFORSK Ltd, Sunndlöra, Norway</mods:affiliation></affiliation></author><author><name sortKey="Hung, S S S" sort="Hung, S S S" uniqKey="Hung S" first="S. S. S." last="Hung">S. S. S. Hung</name><affiliation><mods:affiliation>Department of Animal Science, University of California, Davis, U.S.A.</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Fish Physiology and Biochemistry</title><title level="j" type="abbrev">Fish Physiol Biochem</title><idno type="ISSN">0920-1742</idno><idno type="eISSN">1573-5168</idno><imprint><publisher>Kluwer Academic Publishers</publisher><pubPlace>Dordrecht</pubPlace><date type="published" when="1995-12-01">1995-12-01</date><biblScope unit="volume">14</biblScope><biblScope unit="issue">6</biblScope><biblScope unit="page" from="439">439</biblScope><biblScope unit="page" to="448">448</biblScope></imprint><idno type="ISSN">0920-1742</idno></series><idno type="istex">57F1F83A05B590E77CAE11A60668D1675EC72B38</idno><idno type="DOI">10.1007/BF00004344</idno><idno type="ArticleID">BF00004344</idno><idno type="ArticleID">Art2</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0920-1742</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Enzymatic changes that occur in the white somatic muscle of rainbow trout (Oncorhynchus mykiss) in response to spawning were investigated, and the evenness of their distribution across the ventral-dorsal plane of this muscle was assessed. Four enzymes that are involved in energy metabolism were measured (phosphofructokinase: glycolytic capacity, 3-hydroxyacyl-CoA dehydrogenase: β-oxidation, citrate synthase: citric acid cycle, cytochrome oxidase: oxidative capacity). The enzyme activities were followed in different parts of the white muscle of non-spawning female rainbow trout from May, four months after their first spawning, until December, at second spawning. Samples were taken from white epaxial muscle along the lateral line, on the dorsum, and in between. Samples were also taken from red muscle of non-spawning fish. The isoforms of myosin heavy chains (MyHC) were electrophoretically identified on 6% SDS-PAGE gel to study possible changes in contractile properties of the muscle. Transformation from the non-spawning to spawning phase was associated with dramatic changes in the activity of the enzymes studied in white muscle: glycolytic capacity decreased to less than half, whereas oxidative metabolism increased about two- to four-fold in all areas. Significant quantitative differences in enzyme activities were found between the three epaxial muscle areas: in the non-spawning fish lateral line samples differed from those taken in the other two areas, whereas in spawning fish the dorsal sample difered from the other two. No difference in the expression of MyHC-isoforms was found between spawning and non-spawning fish. Co-expression of both slow and fast isoforms was found in single fibres isolated from red muscle. The results show that the energy metabolism in white muscle of domestic rainbow trout is altered during spawning; i.e., the metabolism becomes increasingly aerobic, with an increased capacity for fatty acid utilization, concomitant with phenotypic changes associated with sexual maturation. These changes are especially pronounced in ventral, superficially located fibres.</div></front></TEI><istex><corpusName>springer</corpusName><author><json:item><name>A. Kiessling</name><affiliations><json:string>Departments of Aquaculture, University of Agricultural Sciences, Uppsala, Sweden</json:string><json:string>Animal Husbandry, University of Agricultural Sciences, Uppsala, Sweden</json:string></affiliations></json:item><json:item><name>L. Larsson</name><affiliations><json:string>Department of Clinical Neurophysiology, Karolinska Hospital, Stockholm, Sweden</json:string></affiliations></json:item><json:item><name>K. -H. 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The enzyme activities were followed in different parts of the white muscle of non-spawning female rainbow trout from May, four months after their first spawning, until December, at second spawning. Samples were taken from white epaxial muscle along the lateral line, on the dorsum, and in between. Samples were also taken from red muscle of non-spawning fish. The isoforms of myosin heavy chains (MyHC) were electrophoretically identified on 6% SDS-PAGE gel to study possible changes in contractile properties of the muscle. Transformation from the non-spawning to spawning phase was associated with dramatic changes in the activity of the enzymes studied in white muscle: glycolytic capacity decreased to less than half, whereas oxidative metabolism increased about two- to four-fold in all areas. Significant quantitative differences in enzyme activities were found between the three epaxial muscle areas: in the non-spawning fish lateral line samples differed from those taken in the other two areas, whereas in spawning fish the dorsal sample difered from the other two. No difference in the expression of MyHC-isoforms was found between spawning and non-spawning fish. Co-expression of both slow and fast isoforms was found in single fibres isolated from red muscle. The results show that the energy metabolism in white muscle of domestic rainbow trout is altered during spawning; i.e., the metabolism becomes increasingly aerobic, with an increased capacity for fatty acid utilization, concomitant with phenotypic changes associated with sexual maturation. These changes are especially pronounced in ventral, superficially located fibres.</abstract><qualityIndicators><score>8</score><pdfVersion>1.3</pdfVersion><pdfPageSize>540 x 738 pts</pdfPageSize><refBibsNative>false</refBibsNative><keywordCount>0</keywordCount><abstractCharCount>2126</abstractCharCount><pdfWordCount>5177</pdfWordCount><pdfCharCount>31685</pdfCharCount><pdfPageCount>10</pdfPageCount><abstractWordCount>304</abstractWordCount></qualityIndicators><title>Spawning induces a shift in energy metabolism from glucose to lipid in rainbow trout white muscle</title><genre><json:string>research-article</json:string></genre><host><volume>14</volume><pages><last>448</last><first>439</first></pages><issn><json:string>0920-1742</json:string></issn><issue>6</issue><subject><json:item><value>Animal Biochemistry</value></json:item><json:item><value>Hydrobiology</value></json:item><json:item><value>Zoology</value></json:item><json:item><value>Animal Anatomy / Morphology / 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Four enzymes that are involved in energy metabolism were measured (phosphofructokinase: glycolytic capacity, 3-hydroxyacyl-CoA dehydrogenase: β-oxidation, citrate synthase: citric acid cycle, cytochrome oxidase: oxidative capacity). The enzyme activities were followed in different parts of the white muscle of non-spawning female rainbow trout from May, four months after their first spawning, until December, at second spawning. Samples were taken from white epaxial muscle along the lateral line, on the dorsum, and in between. Samples were also taken from red muscle of non-spawning fish. The isoforms of myosin heavy chains (MyHC) were electrophoretically identified on 6% SDS-PAGE gel to study possible changes in contractile properties of the muscle. Transformation from the non-spawning to spawning phase was associated with dramatic changes in the activity of the enzymes studied in white muscle: glycolytic capacity decreased to less than half, whereas oxidative metabolism increased about two- to four-fold in all areas. Significant quantitative differences in enzyme activities were found between the three epaxial muscle areas: in the non-spawning fish lateral line samples differed from those taken in the other two areas, whereas in spawning fish the dorsal sample difered from the other two. No difference in the expression of MyHC-isoforms was found between spawning and non-spawning fish. Co-expression of both slow and fast isoforms was found in single fibres isolated from red muscle. The results show that the energy metabolism in white muscle of domestic rainbow trout is altered during spawning; i.e., the metabolism becomes increasingly aerobic, with an increased capacity for fatty acid utilization, concomitant with phenotypic changes associated with sexual maturation. These changes are especially pronounced in ventral, superficially located fibres.</p></abstract><textClass><keywords scheme="Journal Subject"><list><head>Life Sciences</head><item><term>Animal Biochemistry</term></item><item><term>Hydrobiology</term></item><item><term>Zoology</term></item><item><term>Animal Anatomy / Morphology / Histology</term></item><item><term>Animal Physiology</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1995-12-01">Published</change><change xml:id="refBibs-istex" who="#ISTEX-API" when="2016-11-22">References added</change><change xml:id="refBibs-istex" who="#ISTEX-API" when="2017-01-20">References added</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/57F1F83A05B590E77CAE11A60668D1675EC72B38/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Springer, 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ContainsESM="No"><ArticleID>BF00004344</ArticleID><ArticleDOI>10.1007/BF00004344</ArticleDOI><ArticleSequenceNumber>2</ArticleSequenceNumber><ArticleTitle Language="En">Spawning induces a shift in energy metabolism from glucose to lipid in rainbow trout white muscle</ArticleTitle><ArticleFirstPage>439</ArticleFirstPage><ArticleLastPage>448</ArticleLastPage><ArticleHistory><RegistrationDate><Year>2004</Year><Month>3</Month><Day>24</Day></RegistrationDate><Accepted><Year>1995</Year><Month>6</Month><Day>4</Day></Accepted></ArticleHistory><ArticleCopyright><CopyrightHolderName>Kugler Publications</CopyrightHolderName><CopyrightYear>1995</CopyrightYear></ArticleCopyright><ArticleGrants Type="Regular"><MetadataGrant Grant="OpenAccess"></MetadataGrant><AbstractGrant Grant="OpenAccess"></AbstractGrant><BodyPDFGrant Grant="Restricted"></BodyPDFGrant><BodyHTMLGrant Grant="Restricted"></BodyHTMLGrant><BibliographyGrant Grant="Restricted"></BibliographyGrant><ESMGrant Grant="Restricted"></ESMGrant></ArticleGrants><ArticleContext><JournalID>10695</JournalID><VolumeIDStart>14</VolumeIDStart><VolumeIDEnd>14</VolumeIDEnd><IssueIDStart>6</IssueIDStart><IssueIDEnd>6</IssueIDEnd></ArticleContext></ArticleInfo><ArticleHeader><AuthorGroup><Author AffiliationIDS="Aff1 Aff2" CorrespondingAffiliationID="Aff7" PresentAffiliationID="Aff6"><AuthorName DisplayOrder="Western"><GivenName>A.</GivenName><FamilyName>Kiessling</FamilyName></AuthorName><Contact><Phone>+46 18 672116</Phone><Fax>+46 18 672995</Fax></Contact></Author><Author AffiliationIDS="Aff3"><AuthorName DisplayOrder="Western"><GivenName>L.</GivenName><FamilyName>Larsson</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff2"><AuthorName DisplayOrder="Western"><GivenName>K.</GivenName><GivenName>-H.</GivenName><FamilyName>Kiessling</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff5"><AuthorName DisplayOrder="Western"><GivenName>P.</GivenName><GivenName>B.</GivenName><FamilyName>Lutes</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff4"><AuthorName DisplayOrder="Western"><GivenName>T.</GivenName><FamilyName>Storebakken</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff5"><AuthorName DisplayOrder="Western"><GivenName>S.</GivenName><GivenName>S.</GivenName><GivenName>S.</GivenName><FamilyName>Hung</FamilyName></AuthorName></Author><Affiliation ID="Aff1"><OrgDivision>Departments of Aquaculture</OrgDivision><OrgName>University of Agricultural Sciences</OrgName><OrgAddress><City>Uppsala</City><Country>Sweden</Country></OrgAddress></Affiliation><Affiliation ID="Aff2"><OrgDivision>Animal Husbandry</OrgDivision><OrgName>University of Agricultural Sciences</OrgName><OrgAddress><City>Uppsala</City><Country>Sweden</Country></OrgAddress></Affiliation><Affiliation ID="Aff3"><OrgDivision>Department of Clinical Neurophysiology</OrgDivision><OrgName>Karolinska Hospital</OrgName><OrgAddress><City>Stockholm</City><Country>Sweden</Country></OrgAddress></Affiliation><Affiliation ID="Aff4"><OrgName>AKVAFORSK Ltd</OrgName><OrgAddress><City>Sunndlöra</City><Country>Norway</Country></OrgAddress></Affiliation><Affiliation ID="Aff5"><OrgDivision>Department of Animal Science</OrgDivision><OrgName>University of California</OrgName><OrgAddress><City>Davis</City><Country>U.S.A.</Country></OrgAddress></Affiliation><Affiliation ID="Aff6"><OrgDivision>Department of Food Science</OrgDivision><OrgName>University of Agricultural Sciences</OrgName><OrgAddress><Postbox>Box 7024</Postbox><Postcode>S-750 07</Postcode><City>Uppsala</City><Country>Sweden</Country></OrgAddress></Affiliation><Affiliation ID="Aff7"><OrgDivision>Department of Food Science</OrgDivision><OrgName>University of Agriculture Science</OrgName><OrgAddress><Postbox>Box 7024</Postbox><Postcode>S-75007</Postcode><City>Uppsala</City><Country>Sweeden</Country></OrgAddress></Affiliation></AuthorGroup><Abstract ID="Abs1" Language="En"><Heading>Abstract</Heading><Para>Enzymatic changes that occur in the white somatic muscle of rainbow trout (<Emphasis Type="Italic">Oncorhynchus mykiss</Emphasis>) in response to spawning were investigated, and the evenness of their distribution across the ventral-dorsal plane of this muscle was assessed. Four enzymes that are involved in energy metabolism were measured (phosphofructokinase: glycolytic capacity, 3-hydroxyacyl-CoA dehydrogenase: β-oxidation, citrate synthase: citric acid cycle, cytochrome oxidase: oxidative capacity). The enzyme activities were followed in different parts of the white muscle of non-spawning female rainbow trout from May, four months after their first spawning, until December, at second spawning. Samples were taken from white epaxial muscle along the lateral line, on the dorsum, and in between. Samples were also taken from red muscle of non-spawning fish. The isoforms of myosin heavy chains (MyHC) were electrophoretically identified on 6% SDS-PAGE gel to study possible changes in contractile properties of the muscle.</Para><Para>Transformation from the non-spawning to spawning phase was associated with dramatic changes in the activity of the enzymes studied in white muscle: glycolytic capacity decreased to less than half, whereas oxidative metabolism increased about two- to four-fold in all areas. Significant quantitative differences in enzyme activities were found between the three epaxial muscle areas: in the non-spawning fish lateral line samples differed from those taken in the other two areas, whereas in spawning fish the dorsal sample difered from the other two. No difference in the expression of MyHC-isoforms was found between spawning and non-spawning fish. Co-expression of both slow and fast isoforms was found in single fibres isolated from red muscle.</Para><Para>The results show that the energy metabolism in white muscle of domestic rainbow trout is altered during spawning; <Emphasis Type="Italic">i.e.</Emphasis>, the metabolism becomes increasingly aerobic, with an increased capacity for fatty acid utilization, concomitant with phenotypic changes associated with sexual maturation. These changes are especially pronounced in ventral, superficially located fibres.</Para></Abstract><KeywordGroup Language="En"><Heading>Keywords</Heading><Keyword>salmonids</Keyword><Keyword>spawning</Keyword><Keyword>muscle enzyme</Keyword><Keyword>lipid catabolism</Keyword><Keyword>glucose catabolism</Keyword><Keyword>myosin heavy chain</Keyword></KeywordGroup></ArticleHeader><NoBody></NoBody></Article></Issue></Volume></Journal></Publisher></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Spawning induces a shift in energy metabolism from glucose to lipid in rainbow trout white muscle</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Spawning induces a shift in energy metabolism from glucose to lipid in rainbow trout white muscle</title></titleInfo><name type="personal" displayLabel="corresp"><namePart type="given">A.</namePart><namePart type="family">Kiessling</namePart><affiliation>Departments of Aquaculture, University of Agricultural Sciences, Uppsala, Sweden</affiliation><affiliation>Animal Husbandry, University of Agricultural Sciences, Uppsala, Sweden</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">L.</namePart><namePart type="family">Larsson</namePart><affiliation>Department of Clinical Neurophysiology, Karolinska Hospital, Stockholm, Sweden</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">K.</namePart><namePart type="given">-H.</namePart><namePart type="family">Kiessling</namePart><affiliation>Animal Husbandry, University of Agricultural Sciences, Uppsala, Sweden</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">P.</namePart><namePart type="given">B.</namePart><namePart type="family">Lutes</namePart><affiliation>Department of Animal Science, University of California, Davis, U.S.A.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">T.</namePart><namePart type="family">Storebakken</namePart><affiliation>AKVAFORSK Ltd, Sunndlöra, Norway</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S.</namePart><namePart type="given">S.</namePart><namePart type="given">S.</namePart><namePart type="family">Hung</namePart><affiliation>Department of Animal Science, University of California, Davis, U.S.A.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="OriginalPaper"></genre><originInfo><publisher>Kluwer Academic Publishers</publisher><place><placeTerm type="text">Dordrecht</placeTerm></place><dateIssued encoding="w3cdtf">1995-12-01</dateIssued><copyrightDate encoding="w3cdtf">1995</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">Abstract: Enzymatic changes that occur in the white somatic muscle of rainbow trout (Oncorhynchus mykiss) in response to spawning were investigated, and the evenness of their distribution across the ventral-dorsal plane of this muscle was assessed. Four enzymes that are involved in energy metabolism were measured (phosphofructokinase: glycolytic capacity, 3-hydroxyacyl-CoA dehydrogenase: β-oxidation, citrate synthase: citric acid cycle, cytochrome oxidase: oxidative capacity). The enzyme activities were followed in different parts of the white muscle of non-spawning female rainbow trout from May, four months after their first spawning, until December, at second spawning. Samples were taken from white epaxial muscle along the lateral line, on the dorsum, and in between. Samples were also taken from red muscle of non-spawning fish. The isoforms of myosin heavy chains (MyHC) were electrophoretically identified on 6% SDS-PAGE gel to study possible changes in contractile properties of the muscle. Transformation from the non-spawning to spawning phase was associated with dramatic changes in the activity of the enzymes studied in white muscle: glycolytic capacity decreased to less than half, whereas oxidative metabolism increased about two- to four-fold in all areas. Significant quantitative differences in enzyme activities were found between the three epaxial muscle areas: in the non-spawning fish lateral line samples differed from those taken in the other two areas, whereas in spawning fish the dorsal sample difered from the other two. No difference in the expression of MyHC-isoforms was found between spawning and non-spawning fish. Co-expression of both slow and fast isoforms was found in single fibres isolated from red muscle. The results show that the energy metabolism in white muscle of domestic rainbow trout is altered during spawning; i.e., the metabolism becomes increasingly aerobic, with an increased capacity for fatty acid utilization, concomitant with phenotypic changes associated with sexual maturation. These changes are especially pronounced in ventral, superficially located fibres.</abstract><relatedItem type="host"><titleInfo><title>Fish Physiology and Biochemistry</title></titleInfo><titleInfo type="abbreviated"><title>Fish Physiol Biochem</title></titleInfo><genre type="journal" displayLabel="Archive Journal"></genre><originInfo><dateIssued encoding="w3cdtf">1995-12-01</dateIssued><copyrightDate encoding="w3cdtf">1995</copyrightDate></originInfo><subject><genre>Life Sciences</genre><topic>Animal Biochemistry</topic><topic>Hydrobiology</topic><topic>Zoology</topic><topic>Animal Anatomy / Morphology / Histology</topic><topic>Animal Physiology</topic></subject><identifier type="ISSN">0920-1742</identifier><identifier type="eISSN">1573-5168</identifier><identifier type="JournalID">10695</identifier><identifier type="IssueArticleCount">13</identifier><identifier type="VolumeIssueCount">6</identifier><part><date>1995</date><detail type="volume"><number>14</number><caption>vol.</caption></detail><detail type="issue"><number>6</number><caption>no.</caption></detail><extent unit="pages"><start>439</start><end>448</end></extent></part><recordInfo><recordOrigin>Kugler Publications, 1995</recordOrigin></recordInfo></relatedItem><identifier type="istex">57F1F83A05B590E77CAE11A60668D1675EC72B38</identifier><identifier type="DOI">10.1007/BF00004344</identifier><identifier type="ArticleID">BF00004344</identifier><identifier type="ArticleID">Art2</identifier><accessCondition type="use and reproduction" contentType="copyright">Kugler Publications, 1995</accessCondition><recordInfo><recordContentSource>SPRINGER</recordContentSource><recordOrigin>Kugler Publications, 1995</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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