Effect of diets containing a purified soybean trypsin inhibitor on growth performance, digestive proteases and intestinal histology in juvenile sea bream (Sparus aurata L.)
Identifieur interne : 001297 ( Istex/Corpus ); précédent : 001296; suivant : 001298Effect of diets containing a purified soybean trypsin inhibitor on growth performance, digestive proteases and intestinal histology in juvenile sea bream (Sparus aurata L.)
Auteurs : Ester Santigosa ; Miguel Ngel Sáenz De Rodrigá Ez ; Ana Rodiles ; Fernando García Barroso ; Francisco Javier Alarc NSource :
- Aquaculture Research [ 1355-557X ] ; 2010-08.
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Abstract
Juvenile sea bream were fed on diets containing 0.0, 2.0 or 4.0 g kg−1 of a soybean trypsin inhibitor (SBTI) for 30 days. The growth performance, total protease activity and intestinal histology were studied after 0, 15 and 30 days of dietary treatment. No significant differences were found in the weight gain, specific growth rate (SGR) and feed conversion rate in fish fed on inhibitor‐supplemented diets when compared with those fed on an inhibitor‐free diet. Only the SGR at day 15 decreased significantly with protease inhibitor inclusion, although this effect was not observed at day 30. In relation to proteolytic activity at day 15, the total protease activity in the distal intestine decreased in fish fed on inhibitor‐supplemented diets. Zymograms of these extracts showed that the SBTI reduced the intensity of some proteolytic fractions in the distal intestine. A noticeable reduction in the protease activity of the intestinal content in fish fed on the highest level of soybean inhibitor (4.0 g kg−1) was also observed. However, at day 30, the inhibition effect on these active bands was not detected, and the total protease activity was similar to that in fish fed on an inhibitor‐free diet. Histological examination revealed no perceptible differences in the intestinal structure between any of the diet groups. In addition, all fish were maintained under experimentation for 10 more days and fed on an inhibitor‐free diet to determine whether the possible effects caused by the protease inhibitor could be reverted. The administration of SBTI‐supplemented diets did not affect sea bream growth performance or intestine histology after 30 days, and only a decrease in the total alkaline protease activity was found at day 15.
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DOI: 10.1111/j.1365-2109.2010.02500.x
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The growth performance, total protease activity and intestinal histology were studied after 0, 15 and 30 days of dietary treatment. No significant differences were found in the weight gain, specific growth rate (SGR) and feed conversion rate in fish fed on inhibitor‐supplemented diets when compared with those fed on an inhibitor‐free diet. Only the SGR at day 15 decreased significantly with protease inhibitor inclusion, although this effect was not observed at day 30. In relation to proteolytic activity at day 15, the total protease activity in the distal intestine decreased in fish fed on inhibitor‐supplemented diets. Zymograms of these extracts showed that the SBTI reduced the intensity of some proteolytic fractions in the distal intestine. A noticeable reduction in the protease activity of the intestinal content in fish fed on the highest level of soybean inhibitor (4.0 g kg−1) was also observed. However, at day 30, the inhibition effect on these active bands was not detected, and the total protease activity was similar to that in fish fed on an inhibitor‐free diet. Histological examination revealed no perceptible differences in the intestinal structure between any of the diet groups. In addition, all fish were maintained under experimentation for 10 more days and fed on an inhibitor‐free diet to determine whether the possible effects caused by the protease inhibitor could be reverted. The administration of SBTI‐supplemented diets did not affect sea bream growth performance or intestine histology after 30 days, and only a decrease in the total alkaline protease activity was found at day 15.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>Ester Santigosa</name><affiliations><json:string>Department of Physiology, Faculty of Biology, University of Barcelona, Barcelona, Spain</json:string></affiliations></json:item><json:item><name>Miguel Ángel Sáenz de Rodrigáñez</name><affiliations><json:string>Department of Applied Biology, University of Almería, Almería, Spain</json:string></affiliations></json:item><json:item><name>Ana Rodiles</name><affiliations><json:string>IFAPA Aguas del Pino, Cartaya‐Punta Umbría, Huelva, Spain</json:string></affiliations></json:item><json:item><name>Fernando García Barroso</name><affiliations><json:string>Department of Applied Biology, University of Almería, Almería, Spain</json:string></affiliations></json:item><json:item><name>Francisco Javier Alarcón</name><affiliations><json:string>Department of Applied Biology, University of Almería, Almería, Spain</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>digestive proteases</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>gilthead sea bream</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>growth</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>intestine histology</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>SDS‐PAGE</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>trypsin inhibitor</value></json:item></subject><articleId><json:string>ARE2500</json:string></articleId><language><json:string>eng</json:string></language><originalGenre><json:string>article</json:string></originalGenre><abstract>Juvenile sea bream were fed on diets containing 0.0, 2.0 or 4.0 g kg−1 of a soybean trypsin inhibitor (SBTI) for 30 days. 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However, at day 30, the inhibition effect on these active bands was not detected, and the total protease activity was similar to that in fish fed on an inhibitor‐free diet. Histological examination revealed no perceptible differences in the intestinal structure between any of the diet groups. In addition, all fish were maintained under experimentation for 10 more days and fed on an inhibitor‐free diet to determine whether the possible effects caused by the protease inhibitor could be reverted. 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Journal Compilation © 2010 Blackwell Publishing Ltd</p></availability><date>2010</date></publicationStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Effect of diets containing a purified soybean trypsin inhibitor on growth performance, digestive proteases and intestinal histology in juvenile sea bream (Sparus aurata L.)</title><author xml:id="author-1"><persName><forename type="first">Ester</forename><surname>Santigosa</surname></persName><note type="biography">* Present address: E Santigosa, Ifremer, UMR 1067 Fish Nutrition Unit, BP 70, 29280 Plouzane, France.</note><affiliation>* Present address: E Santigosa, Ifremer, UMR 1067 Fish Nutrition Unit, BP 70, 29280 Plouzane, France.</affiliation><affiliation>Department of Physiology, Faculty of Biology, University of Barcelona, Barcelona, Spain</affiliation></author><author xml:id="author-2"><persName><forename type="first">Miguel Ángel</forename><surname>Sáenz de Rodrigáñez</surname></persName><affiliation>Department of Applied Biology, University of Almería, Almería, Spain</affiliation></author><author xml:id="author-3"><persName><forename type="first">Ana</forename><surname>Rodiles</surname></persName><affiliation>IFAPA Aguas del Pino, Cartaya‐Punta Umbría, Huelva, Spain</affiliation></author><author xml:id="author-4"><persName><forename type="first">Fernando García</forename><surname>Barroso</surname></persName><affiliation>Department of Applied Biology, University of Almería, Almería, Spain</affiliation></author><author xml:id="author-5"><persName><forename type="first">Francisco Javier</forename><surname>Alarcón</surname></persName><affiliation>Department of Applied Biology, University of Almería, Almería, Spain</affiliation></author></analytic><monogr><title level="j">Aquaculture Research</title><idno type="pISSN">1355-557X</idno><idno type="eISSN">1365-2109</idno><idno type="DOI">10.1111/(ISSN)1365-2109</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2010-08"></date><biblScope unit="volume">41</biblScope><biblScope unit="issue">9</biblScope><biblScope unit="page" from="187">e187</biblScope><biblScope unit="page" to="198">e198</biblScope></imprint></monogr><idno type="istex">9A06B7B2FA606EDFCF6F5AB50EE4B980667A4EC2</idno><idno type="DOI">10.1111/j.1365-2109.2010.02500.x</idno><idno type="ArticleID">ARE2500</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2010</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Juvenile sea bream were fed on diets containing 0.0, 2.0 or 4.0 g kg−1 of a soybean trypsin inhibitor (SBTI) for 30 days. The growth performance, total protease activity and intestinal histology were studied after 0, 15 and 30 days of dietary treatment. No significant differences were found in the weight gain, specific growth rate (SGR) and feed conversion rate in fish fed on inhibitor‐supplemented diets when compared with those fed on an inhibitor‐free diet. Only the SGR at day 15 decreased significantly with protease inhibitor inclusion, although this effect was not observed at day 30. In relation to proteolytic activity at day 15, the total protease activity in the distal intestine decreased in fish fed on inhibitor‐supplemented diets. Zymograms of these extracts showed that the SBTI reduced the intensity of some proteolytic fractions in the distal intestine. A noticeable reduction in the protease activity of the intestinal content in fish fed on the highest level of soybean inhibitor (4.0 g kg−1) was also observed. However, at day 30, the inhibition effect on these active bands was not detected, and the total protease activity was similar to that in fish fed on an inhibitor‐free diet. Histological examination revealed no perceptible differences in the intestinal structure between any of the diet groups. In addition, all fish were maintained under experimentation for 10 more days and fed on an inhibitor‐free diet to determine whether the possible effects caused by the protease inhibitor could be reverted. The administration of SBTI‐supplemented diets did not affect sea bream growth performance or intestine histology after 30 days, and only a decrease in the total alkaline protease activity was found at day 15.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>keywords</head><item><term>digestive proteases</term></item><item><term>gilthead sea bream</term></item><item><term>growth</term></item><item><term>intestine histology</term></item><item><term>SDS‐PAGE</term></item><item><term>trypsin inhibitor</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2010-08">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/9A06B7B2FA606EDFCF6F5AB50EE4B980667A4EC2/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>Blackwell Publishing Ltd</publisherName><publisherLoc>Oxford, UK</publisherLoc></publisherInfo><doi origin="wiley" registered="yes">10.1111/(ISSN)1365-2109</doi><issn type="print">1355-557X</issn><issn type="electronic">1365-2109</issn><idGroup><id type="product" value="ARE"></id><id type="publisherDivision" value="ST"></id></idGroup><titleGroup><title type="main" sort="AQUACULTURE RESEARCH">Aquaculture Research</title></titleGroup></publicationMeta><publicationMeta level="part" position="08109"><doi origin="wiley">10.1111/are.2010.41.issue-9</doi><numberingGroup><numbering type="journalVolume" number="41">41</numbering><numbering type="journalIssue" number="9">9</numbering></numberingGroup><coverDate startDate="2010-08">August 2010</coverDate></publicationMeta><publicationMeta level="unit" type="article" position="34" status="forIssue"><doi origin="wiley">10.1111/j.1365-2109.2010.02500.x</doi><idGroup><id type="unit" value="ARE2500"></id><id type="supplier" value="2500"></id></idGroup><countGroup><count type="pageTotal" number="12"></count></countGroup><titleGroup><title type="tocHeading1">Original Articles</title></titleGroup><copyright>© 2010 The Authors. Journal Compilation © 2010 Blackwell Publishing Ltd</copyright><eventGroup><event type="firstOnline" date="2010-04-27"></event><event type="publishedOnlineAcceptedOrEarlyUnpaginated" date="2010-04-27"></event><event type="xmlConverted" agent="Converter:BPG_TO_WML3G version:2.3.25 mode:FullText source:FullText result:FullText" date="2010-11-10"></event><event type="publishedOnlineFinalForm" date="2010-08-19"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:3.8.8" date="2014-01-06"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-10-15"></event></eventGroup><numberingGroup><numbering type="pageFirst">e187</numbering><numbering type="pageLast">e198</numbering></numberingGroup><correspondenceTo><b>Correspondence:</b> F J Alarcón, Department of Applied Biology, University of Almería, 04120 Almería, Spain. E‐mail: <email normalForm="falarcon@ual.es">falarcon@ual.es</email></correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:ARE.ARE2500.pdf"></link></linkGroup></publicationMeta><contentMeta><countGroup><count type="figureTotal" number="5"></count><count type="tableTotal" number="2"></count><count type="formulaTotal" number="0"></count><count type="referenceTotal" number="51"></count><count type="wordTotal" number="7655"></count><count type="linksCrossRef" number="86"></count></countGroup><titleGroup><title type="main">Effect of diets containing a purified soybean trypsin inhibitor on growth performance, digestive proteases and intestinal histology in juvenile sea bream (<i>Sparus aurata</i> L.)</title><title type="shortAuthors">E Santigosa <i>et al.</i></title><title type="short">Effect of SBTI on growth and digestive proteases of sea bream</title></titleGroup><creators><creator creatorRole="author" xml:id="cr1" affiliationRef="#a1" noteRef="#fn1"><personName><givenNames>Ester</givenNames><familyName>Santigosa</familyName></personName></creator><creator creatorRole="author" xml:id="cr2" affiliationRef="#a2"><personName><givenNames>Miguel Ángel</givenNames><familyName>Sáenz de Rodrigáñez</familyName></personName></creator><creator creatorRole="author" xml:id="cr3" affiliationRef="#a3"><personName><givenNames>Ana</givenNames><familyName>Rodiles</familyName></personName></creator><creator creatorRole="author" xml:id="cr4" affiliationRef="#a2"><personName><givenNames>Fernando García</givenNames><familyName>Barroso</familyName></personName></creator><creator creatorRole="author" xml:id="cr5" affiliationRef="#a2"><personName><givenNames>Francisco Javier</givenNames><familyName>Alarcón</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="a1" countryCode="ES"><unparsedAffiliation>Department of Physiology, Faculty of Biology, University of Barcelona, Barcelona, Spain</unparsedAffiliation></affiliation><affiliation xml:id="a2" countryCode="ES"><unparsedAffiliation>Department of Applied Biology, University of Almería, Almería, Spain</unparsedAffiliation></affiliation><affiliation xml:id="a3" countryCode="ES"><unparsedAffiliation>IFAPA Aguas del Pino, Cartaya‐Punta Umbría, Huelva, Spain</unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en"><keyword xml:id="k1">digestive proteases</keyword><keyword xml:id="k2">gilthead sea bream</keyword><keyword xml:id="k3">growth</keyword><keyword xml:id="k4">intestine histology</keyword><keyword xml:id="k5">SDS‐PAGE</keyword><keyword xml:id="k6">trypsin inhibitor</keyword></keywordGroup><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Abstract</title><p>Juvenile sea bream were fed on diets containing 0.0, 2.0 or 4.0 g kg<sup>−1</sup> of a soybean trypsin inhibitor (SBTI) for 30 days. The growth performance, total protease activity and intestinal histology were studied after 0, 15 and 30 days of dietary treatment. No significant differences were found in the weight gain, specific growth rate (SGR) and feed conversion rate in fish fed on inhibitor‐supplemented diets when compared with those fed on an inhibitor‐free diet. Only the SGR at day 15 decreased significantly with protease inhibitor inclusion, although this effect was not observed at day 30. In relation to proteolytic activity at day 15, the total protease activity in the distal intestine decreased in fish fed on inhibitor‐supplemented diets. Zymograms of these extracts showed that the SBTI reduced the intensity of some proteolytic fractions in the distal intestine. A noticeable reduction in the protease activity of the intestinal content in fish fed on the highest level of soybean inhibitor (4.0 g kg<sup>−1</sup>) was also observed. However, at day 30, the inhibition effect on these active bands was not detected, and the total protease activity was similar to that in fish fed on an inhibitor‐free diet. Histological examination revealed no perceptible differences in the intestinal structure between any of the diet groups. In addition, all fish were maintained under experimentation for 10 more days and fed on an inhibitor‐free diet to determine whether the possible effects caused by the protease inhibitor could be reverted. The administration of SBTI‐supplemented diets did not affect sea bream growth performance or intestine histology after 30 days, and only a decrease in the total alkaline protease activity was found at day 15.</p></abstract></abstractGroup></contentMeta><noteGroup><note xml:id="fn1"><p><sup>*</sup><b>Present address:</b> E Santigosa, Ifremer, UMR 1067 Fish Nutrition Unit, BP 70, 29280 Plouzane, France.</p></note></noteGroup></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Effect of diets containing a purified soybean trypsin inhibitor on growth performance, digestive proteases and intestinal histology in juvenile sea bream (Sparus aurata L.)</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>Effect of SBTI on growth and digestive proteases of sea bream</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Effect of diets containing a purified soybean trypsin inhibitor on growth performance, digestive proteases and intestinal histology in juvenile sea bream (Sparus aurata L.)</title></titleInfo><name type="personal"><namePart type="given">Ester</namePart><namePart type="family">Santigosa</namePart><affiliation>Department of Physiology, Faculty of Biology, University of Barcelona, Barcelona, Spain</affiliation><description>* Present address: E Santigosa, Ifremer, UMR 1067 Fish Nutrition Unit, BP 70, 29280 Plouzane, France.</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Miguel Ángel</namePart><namePart type="family">Sáenz de Rodrigáñez</namePart><affiliation>Department of Applied Biology, University of Almería, Almería, Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Ana</namePart><namePart type="family">Rodiles</namePart><affiliation>IFAPA Aguas del Pino, Cartaya‐Punta Umbría, Huelva, Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Fernando García</namePart><namePart type="family">Barroso</namePart><affiliation>Department of Applied Biology, University of Almería, Almería, Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Francisco Javier</namePart><namePart type="family">Alarcón</namePart><affiliation>Department of Applied Biology, University of Almería, Almería, Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Blackwell Publishing Ltd</publisher><place><placeTerm type="text">Oxford, UK</placeTerm></place><dateIssued encoding="w3cdtf">2010-08</dateIssued><copyrightDate encoding="w3cdtf">2010</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">5</extent><extent unit="tables">2</extent><extent unit="references">51</extent><extent unit="words">7655</extent></physicalDescription><abstract lang="en">Juvenile sea bream were fed on diets containing 0.0, 2.0 or 4.0 g kg−1 of a soybean trypsin inhibitor (SBTI) for 30 days. The growth performance, total protease activity and intestinal histology were studied after 0, 15 and 30 days of dietary treatment. No significant differences were found in the weight gain, specific growth rate (SGR) and feed conversion rate in fish fed on inhibitor‐supplemented diets when compared with those fed on an inhibitor‐free diet. Only the SGR at day 15 decreased significantly with protease inhibitor inclusion, although this effect was not observed at day 30. In relation to proteolytic activity at day 15, the total protease activity in the distal intestine decreased in fish fed on inhibitor‐supplemented diets. Zymograms of these extracts showed that the SBTI reduced the intensity of some proteolytic fractions in the distal intestine. A noticeable reduction in the protease activity of the intestinal content in fish fed on the highest level of soybean inhibitor (4.0 g kg−1) was also observed. However, at day 30, the inhibition effect on these active bands was not detected, and the total protease activity was similar to that in fish fed on an inhibitor‐free diet. Histological examination revealed no perceptible differences in the intestinal structure between any of the diet groups. In addition, all fish were maintained under experimentation for 10 more days and fed on an inhibitor‐free diet to determine whether the possible effects caused by the protease inhibitor could be reverted. The administration of SBTI‐supplemented diets did not affect sea bream growth performance or intestine histology after 30 days, and only a decrease in the total alkaline protease activity was found at day 15.</abstract><subject lang="en"><genre>keywords</genre><topic>digestive proteases</topic><topic>gilthead sea bream</topic><topic>growth</topic><topic>intestine histology</topic><topic>SDS‐PAGE</topic><topic>trypsin inhibitor</topic></subject><relatedItem type="host"><titleInfo><title>Aquaculture Research</title></titleInfo><genre type="journal">journal</genre><identifier type="ISSN">1355-557X</identifier><identifier type="eISSN">1365-2109</identifier><identifier type="DOI">10.1111/(ISSN)1365-2109</identifier><identifier type="PublisherID">ARE</identifier><part><date>2010</date><detail type="volume"><caption>vol.</caption><number>41</number></detail><detail type="issue"><caption>no.</caption><number>9</number></detail><extent unit="pages"><start>e187</start><end>e198</end><total>12</total></extent></part></relatedItem><identifier type="istex">9A06B7B2FA606EDFCF6F5AB50EE4B980667A4EC2</identifier><identifier type="DOI">10.1111/j.1365-2109.2010.02500.x</identifier><identifier type="ArticleID">ARE2500</identifier><accessCondition type="use and reproduction" contentType="copyright">© 2010 The Authors. 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