Exposure of three generations of the estuarine sheepshead minnow (Cyprinodon variegatus) to the androgen, 17β‐trenbolone: Effects on survival, development, and reproduction
Identifieur interne : 001284 ( Istex/Corpus ); précédent : 001283; suivant : 001285Exposure of three generations of the estuarine sheepshead minnow (Cyprinodon variegatus) to the androgen, 17β‐trenbolone: Effects on survival, development, and reproduction
Auteurs : Geraldine M. Cripe ; Becky L. Hemmer ; Sandy Raimondo ; Larry R. Goodman ; Dannielle H. KulawSource :
- Environmental Toxicology and Chemistry [ 0730-7268 ] ; 2010-09.
English descriptors
Abstract
Estimating long‐term effects of endocrine‐disrupting chemicals on a species is important to assessing the overall risk to the populations. The present study reports the results of a 42‐week exposure of estuarine sheepshead minnows (Cyprinodon variegatus) to the androgen, 17β‐trenbolone (Tb) conducted to determine if partial‐(F0) or single‐generation (F1) fish exposures identify multigenerational (F0–F3) effects of androgens on fish. Adult F0 fish were exposed to 0.007, 0.027, 0.13, 0.87,and 4.1 µg Tb/L, the F1 generation to ≤0.87 µg Tb/L, the F2 fish to ≤0.13 µg Tb/L, and the F3 fish to ≤0.027 µg Tb/L. The highest concentrations with reproducing populations at the end of the F0, F1, and F2 generations were 4.1, 0.87, and 0.027 µg Tb/L, respectively. Reproduction in the F0, F1, and F2 generations was significantly reduced at 0.87, 0.027, and 0.027 µg Tb/L, respectively. Fish were significantly masculinized in the F1 generation exposed to 0.13 µg Tb/L or greater. Female plasma vitellogenin was significantly reduced in F0 fish exposed to ≥0.87 µg Tb/L. Gonadosomatic indices of the F0 and F1 generations were significantly increased at 0.87 and 0.13 µg Tb/L in the F0 and F1 generation, respectively, and were accompanied by ovarian histological changes. Reproduction was the most consistently sensitive measure of androgen effects and, after a life‐cycle exposure, the daily reproductive rate predicted concentrations affecting successive generations. The present study provides evidence that a multiple generation exposure of fish to some endocrine‐disrupting chemicals can result in developmental and reproductive changes that have a much greater impact on the success of a species than was indicated from shorter term exposures. Environ. Toxicol. Chem. 2010;29:2079–2087. © 2010 SETAC
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DOI: 10.1002/etc.261
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ISTEX:F842D993782BBB15410C20EDC29A6A5DFF9830A8Le document en format XML
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The present study reports the results of a 42‐week exposure of estuarine sheepshead minnows (Cyprinodon variegatus) to the androgen, 17β‐trenbolone (Tb) conducted to determine if partial‐(F0) or single‐generation (F1) fish exposures identify multigenerational (F0–F3) effects of androgens on fish. Adult F0 fish were exposed to 0.007, 0.027, 0.13, 0.87,and 4.1 µg Tb/L, the F1 generation to ≤0.87 µg Tb/L, the F2 fish to ≤0.13 µg Tb/L, and the F3 fish to ≤0.027 µg Tb/L. The highest concentrations with reproducing populations at the end of the F0, F1, and F2 generations were 4.1, 0.87, and 0.027 µg Tb/L, respectively. Reproduction in the F0, F1, and F2 generations was significantly reduced at 0.87, 0.027, and 0.027 µg Tb/L, respectively. Fish were significantly masculinized in the F1 generation exposed to 0.13 µg Tb/L or greater. Female plasma vitellogenin was significantly reduced in F0 fish exposed to ≥0.87 µg Tb/L. Gonadosomatic indices of the F0 and F1 generations were significantly increased at 0.87 and 0.13 µg Tb/L in the F0 and F1 generation, respectively, and were accompanied by ovarian histological changes. Reproduction was the most consistently sensitive measure of androgen effects and, after a life‐cycle exposure, the daily reproductive rate predicted concentrations affecting successive generations. The present study provides evidence that a multiple generation exposure of fish to some endocrine‐disrupting chemicals can result in developmental and reproductive changes that have a much greater impact on the success of a species than was indicated from shorter term exposures. Environ. Toxicol. Chem. 2010;29:2079–2087. © 2010 SETAC</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>Geraldine M. 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Gonadosomatic indices of the F0 and F1 generations were significantly increased at 0.87 and 0.13 µg Tb/L in the F0 and F1 generation, respectively, and were accompanied by ovarian histological changes. Reproduction was the most consistently sensitive measure of androgen effects and, after a life‐cycle exposure, the daily reproductive rate predicted concentrations affecting successive generations. The present study provides evidence that a multiple generation exposure of fish to some endocrine‐disrupting chemicals can result in developmental and reproductive changes that have a much greater impact on the success of a species than was indicated from shorter term exposures. Environ. Toxicol. 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type="wordTotal" number="8316"></count></countGroup><titleGroup><title type="main" xml:lang="en">Exposure of three generations of the estuarine sheepshead minnow (<i>Cyprinodon variegatus</i>) to the androgen, 17β‐trenbolone: Effects on survival, development, and reproduction</title><title type="short" xml:lang="en">Trenbolone effects on three generations of sheepshead minnow</title></titleGroup><creators><creator xml:id="au1" creatorRole="author" affiliationRef="#af1"><personName><givenNames>Geraldine M.</givenNames><familyName>Cripe</familyName></personName></creator><creator xml:id="au2" creatorRole="author" affiliationRef="#af1"><personName><givenNames>Becky L.</givenNames><familyName>Hemmer</familyName></personName></creator><creator xml:id="au3" creatorRole="author" affiliationRef="#af1" corresponding="yes"><personName><givenNames>Sandy</givenNames><familyName>Raimondo</familyName></personName><contactDetails><email>raimondo.sandy@epa.gov</email></contactDetails></creator><creator xml:id="au4" creatorRole="author" affiliationRef="#af1"><personName><givenNames>Larry R.</givenNames><familyName>Goodman</familyName></personName></creator><creator xml:id="au5" creatorRole="author" affiliationRef="#af2"><personName><givenNames>Dannielle H.</givenNames><familyName>Kulaw</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="af1" countryCode="US" type="organization"><unparsedAffiliation>U.S. Environmental Protection Agency, National Health and Environmental Effects Research Laboratory, Gulf Ecology Division, 1 Sabine Island Drive, Gulf Breeze, Florida 32561</unparsedAffiliation></affiliation><affiliation xml:id="af2" countryCode="US" type="organization"><unparsedAffiliation>Louisiana State University, Baton Rouge, Louisiana 70803, USA</unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en" type="author"><keyword xml:id="kwd1">17β‐Trenbolone</keyword><keyword xml:id="kwd2">Sheepshead minnow</keyword><keyword xml:id="kwd3">Multiple generation</keyword><keyword xml:id="kwd4">Endocrine disruption</keyword></keywordGroup><supportingInformation><p> All Supplemental Data may be found in the online version of this article. </p><supportingInfoItem><mediaResource alt="supporting information" href="urn-x:wiley:07307268:media:etc261:261_etc_sm_suppTab"></mediaResource><caption>Supplementary Table</caption></supportingInfoItem></supportingInformation><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Abstract</title><p>Estimating long‐term effects of endocrine‐disrupting chemicals on a species is important to assessing the overall risk to the populations. The present study reports the results of a 42‐week exposure of estuarine sheepshead minnows (<i>Cyprinodon variegatus</i>) to the androgen, 17β‐trenbolone (Tb) conducted to determine if partial‐(F0) or single‐generation (F1) fish exposures identify multigenerational (F0–F3) effects of androgens on fish. Adult F0 fish were exposed to 0.007, 0.027, 0.13, 0.87,and 4.1 µg Tb/L, the F1 generation to ≤0.87 µg Tb/L, the F2 fish to ≤0.13 µg Tb/L, and the F3 fish to ≤0.027 µg Tb/L. The highest concentrations with reproducing populations at the end of the F0, F1, and F2 generations were 4.1, 0.87, and 0.027 µg Tb/L, respectively. Reproduction in the F0, F1, and F2 generations was significantly reduced at 0.87, 0.027, and 0.027 µg Tb/L, respectively. Fish were significantly masculinized in the F1 generation exposed to 0.13 µg Tb/L or greater. Female plasma vitellogenin was significantly reduced in F0 fish exposed to ≥0.87 µg Tb/L. Gonadosomatic indices of the F0 and F1 generations were significantly increased at 0.87 and 0.13 µg Tb/L in the F0 and F1 generation, respectively, and were accompanied by ovarian histological changes. Reproduction was the most consistently sensitive measure of androgen effects and, after a life‐cycle exposure, the daily reproductive rate predicted concentrations affecting successive generations. The present study provides evidence that a multiple generation exposure of fish to some endocrine‐disrupting chemicals can result in developmental and reproductive changes that have a much greater impact on the success of a species than was indicated from shorter term exposures. Environ. Toxicol. Chem. 2010;29:2079–2087. © 2010 SETAC</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Exposure of three generations of the estuarine sheepshead minnow (Cyprinodon variegatus) to the androgen, 17β‐trenbolone: Effects on survival, development, and reproduction</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>Trenbolone effects on three generations of sheepshead minnow</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Exposure of three generations of the estuarine sheepshead minnow (Cyprinodon variegatus) to the androgen, 17β‐trenbolone: Effects on survival, development, and reproduction</title></titleInfo><name type="personal"><namePart type="given">Geraldine M.</namePart><namePart type="family">Cripe</namePart><affiliation>U.S. Environmental Protection Agency, National Health and Environmental Effects Research Laboratory, Gulf Ecology Division, 1 Sabine Island Drive, Gulf Breeze, Florida 32561</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Becky L.</namePart><namePart type="family">Hemmer</namePart><affiliation>U.S. Environmental Protection Agency, National Health and Environmental Effects Research Laboratory, Gulf Ecology Division, 1 Sabine Island Drive, Gulf Breeze, Florida 32561</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Sandy</namePart><namePart type="family">Raimondo</namePart><affiliation>U.S. Environmental Protection Agency, National Health and Environmental Effects Research Laboratory, Gulf Ecology Division, 1 Sabine Island Drive, Gulf Breeze, Florida 32561</affiliation><affiliation>U.S. Environmental Protection Agency, National Health and Environmental Effects Research Laboratory, Gulf Ecology Division, 1 Sabine Island Drive, Gulf Breeze, Florida 32561.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Larry R.</namePart><namePart type="family">Goodman</namePart><affiliation>U.S. Environmental Protection Agency, National Health and Environmental Effects Research Laboratory, Gulf Ecology Division, 1 Sabine Island Drive, Gulf Breeze, Florida 32561</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Dannielle H.</namePart><namePart type="family">Kulaw</namePart><affiliation>Louisiana State University, Baton Rouge, Louisiana 70803, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>John Wiley & Sons, Inc.</publisher><place><placeTerm type="text">Hoboken, USA</placeTerm></place><dateIssued encoding="w3cdtf">2010-09</dateIssued><dateCaptured encoding="w3cdtf">2009-10-28</dateCaptured><dateValid encoding="w3cdtf">2010-04-01</dateValid><copyrightDate encoding="w3cdtf">2010</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">4</extent><extent unit="tables">3</extent><extent unit="references">32</extent><extent unit="words">8316</extent></physicalDescription><abstract lang="en">Estimating long‐term effects of endocrine‐disrupting chemicals on a species is important to assessing the overall risk to the populations. The present study reports the results of a 42‐week exposure of estuarine sheepshead minnows (Cyprinodon variegatus) to the androgen, 17β‐trenbolone (Tb) conducted to determine if partial‐(F0) or single‐generation (F1) fish exposures identify multigenerational (F0–F3) effects of androgens on fish. Adult F0 fish were exposed to 0.007, 0.027, 0.13, 0.87,and 4.1 µg Tb/L, the F1 generation to ≤0.87 µg Tb/L, the F2 fish to ≤0.13 µg Tb/L, and the F3 fish to ≤0.027 µg Tb/L. The highest concentrations with reproducing populations at the end of the F0, F1, and F2 generations were 4.1, 0.87, and 0.027 µg Tb/L, respectively. Reproduction in the F0, F1, and F2 generations was significantly reduced at 0.87, 0.027, and 0.027 µg Tb/L, respectively. Fish were significantly masculinized in the F1 generation exposed to 0.13 µg Tb/L or greater. Female plasma vitellogenin was significantly reduced in F0 fish exposed to ≥0.87 µg Tb/L. Gonadosomatic indices of the F0 and F1 generations were significantly increased at 0.87 and 0.13 µg Tb/L in the F0 and F1 generation, respectively, and were accompanied by ovarian histological changes. Reproduction was the most consistently sensitive measure of androgen effects and, after a life‐cycle exposure, the daily reproductive rate predicted concentrations affecting successive generations. The present study provides evidence that a multiple generation exposure of fish to some endocrine‐disrupting chemicals can result in developmental and reproductive changes that have a much greater impact on the success of a species than was indicated from shorter term exposures. Environ. Toxicol. Chem. 2010;29:2079–2087. © 2010 SETAC</abstract><subject lang="en"><genre>keywords</genre><topic>17β‐Trenbolone</topic><topic>Sheepshead minnow</topic><topic>Multiple generation</topic><topic>Endocrine disruption</topic></subject><relatedItem type="host"><titleInfo><title>Environmental Toxicology and Chemistry</title></titleInfo><titleInfo type="abbreviated"><title>Environmental Toxicology and Chemistry</title></titleInfo><genre type="journal">journal</genre><note type="content"> All Supplemental Data may be found in the online version of this article.Supporting Info Item: Supplementary Table - </note><subject><genre>article-category</genre><topic>Environmental Toxicology</topic></subject><identifier type="ISSN">0730-7268</identifier><identifier type="eISSN">1552-8618</identifier><identifier type="DOI">10.1002/(ISSN)1552-8618</identifier><identifier type="PublisherID">ETC</identifier><part><date>2010</date><detail type="volume"><caption>vol.</caption><number>29</number></detail><detail type="issue"><caption>no.</caption><number>9</number></detail><extent unit="pages"><start>2079</start><end>2087</end><total>9</total></extent></part></relatedItem><identifier type="istex">F842D993782BBB15410C20EDC29A6A5DFF9830A8</identifier><identifier type="DOI">10.1002/etc.261</identifier><identifier type="ArticleID">ETC261</identifier><accessCondition type="use and reproduction" contentType="copyright">Copyright © 2010 SETAC</accessCondition><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>John Wiley & Sons, Inc.</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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