Use of laparoscopy to determine sex and reproductive status of shortnose sturgeon (Acipenser brevirostrum) and Atlantic sturgeon (Acipenser oxyrinchus oxyrinchus)
Identifieur interne : 001271 ( Istex/Corpus ); précédent : 001270; suivant : 001272Use of laparoscopy to determine sex and reproductive status of shortnose sturgeon (Acipenser brevirostrum) and Atlantic sturgeon (Acipenser oxyrinchus oxyrinchus)
Auteurs : M. A. Matsche ; R. S. Bakal ; K. M. RosemarySource :
- Journal of Applied Ichthyology [ 0175-8659 ] ; 2011-04.
Abstract
Sex and reproductive maturity of Atlantic and shortnose sturgeon were determined by visual examination of the gonads using laparoscopy, and were validated by histological examination of gonadal biopsies. Surgical anesthesia was induced in all fish with 250 mg L−1 tricaine methanesulfonate (MS‐222) and maintained throughout procedures with 85 mg L−1 MS‐222 on a mobile surgical cart. A pair of Ternamian EndoTip cannulae installed through the ventral body wall in each fish, allowed access for a 5‐mm rigid laparoscope and biopsy forceps. Video endocamera use with the laparoscope, following air insufflation of the coelom, provided detailed, high quality imagery to aspirate the swim bladder, examine the gonad and collect biopsies without inducing iatrogenic trauma. Germinal tissue of all immature males, 25% of immature female shortnose sturgeon and 45% of immature female Atlantic sturgeon were concealed by fat preventing sex determination by visual assessment. Morphological features of gonads were used to determine sex in all remaining fish and were 100% in concordance with histological findings. Relative amount of gonadal fat; gonad size and color; presence of testicular lobes or ovarian lamellae; and color, size and density of oocytes were useful in determining reproductive stage. Gonad morphology of each reproductive stage was similar in Atlantic and shortnose sturgeon. All captive Atlantic sturgeon survived laparoscopy, gained weight at the same rate as unexamined fish and scars from incisions were no longer evident 9–12 months after surgery. Laparoscopic procedures presented here offer a safe and highly reliable way to determine sex and reproductive status for Atlantic and shortnose sturgeon.
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DOI: 10.1111/j.1439-0426.2011.01679.x
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Surgical anesthesia was induced in all fish with 250 mg L−1 tricaine methanesulfonate (MS‐222) and maintained throughout procedures with 85 mg L−1 MS‐222 on a mobile surgical cart. A pair of Ternamian EndoTip cannulae installed through the ventral body wall in each fish, allowed access for a 5‐mm rigid laparoscope and biopsy forceps. Video endocamera use with the laparoscope, following air insufflation of the coelom, provided detailed, high quality imagery to aspirate the swim bladder, examine the gonad and collect biopsies without inducing iatrogenic trauma. Germinal tissue of all immature males, 25% of immature female shortnose sturgeon and 45% of immature female Atlantic sturgeon were concealed by fat preventing sex determination by visual assessment. Morphological features of gonads were used to determine sex in all remaining fish and were 100% in concordance with histological findings. Relative amount of gonadal fat; gonad size and color; presence of testicular lobes or ovarian lamellae; and color, size and density of oocytes were useful in determining reproductive stage. Gonad morphology of each reproductive stage was similar in Atlantic and shortnose sturgeon. All captive Atlantic sturgeon survived laparoscopy, gained weight at the same rate as unexamined fish and scars from incisions were no longer evident 9–12 months after surgery. Laparoscopic procedures presented here offer a safe and highly reliable way to determine sex and reproductive status for Atlantic and shortnose sturgeon.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>M. A. Matsche</name><affiliations><json:string>Cooperative Oxford Laboratory, Oxford, MD, USA</json:string></affiliations></json:item><json:item><name>R. S. 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A pair of Ternamian EndoTip cannulae installed through the ventral body wall in each fish, allowed access for a 5‐mm rigid laparoscope and biopsy forceps. Video endocamera use with the laparoscope, following air insufflation of the coelom, provided detailed, high quality imagery to aspirate the swim bladder, examine the gonad and collect biopsies without inducing iatrogenic trauma. Germinal tissue of all immature males, 25% of immature female shortnose sturgeon and 45% of immature female Atlantic sturgeon were concealed by fat preventing sex determination by visual assessment. Morphological features of gonads were used to determine sex in all remaining fish and were 100% in concordance with histological findings. Relative amount of gonadal fat; gonad size and color; presence of testicular lobes or ovarian lamellae; and color, size and density of oocytes were useful in determining reproductive stage. Gonad morphology of each reproductive stage was similar in Atlantic and shortnose sturgeon. All captive Atlantic sturgeon survived laparoscopy, gained weight at the same rate as unexamined fish and scars from incisions were no longer evident 9–12 months after surgery. 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A.</forename><surname>Matsche</surname></persName><affiliation>Cooperative Oxford Laboratory, Oxford, MD, USA</affiliation></author><author xml:id="author-2"><persName><forename type="first">R. S.</forename><surname>Bakal</surname></persName><affiliation>United States Fish and Wildlife Service, Raleigh, NC, USA</affiliation></author><author xml:id="author-3"><persName><forename type="first">K. M.</forename><surname>Rosemary</surname></persName><affiliation>Cooperative Oxford Laboratory, Oxford, MD, USA</affiliation></author></analytic><monogr><title level="j">Journal of Applied Ichthyology</title><idno type="pISSN">0175-8659</idno><idno type="eISSN">1439-0426</idno><idno type="DOI">10.1111/(ISSN)1439-0426</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2011-04"></date><biblScope unit="volume">27</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="627">627</biblScope><biblScope unit="page" to="636">636</biblScope></imprint></monogr><idno type="istex">846A72867B3945DC7967CED861A9E26205B8441C</idno><idno type="DOI">10.1111/j.1439-0426.2011.01679.x</idno><idno type="ArticleID">JAI1679</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2011</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Sex and reproductive maturity of Atlantic and shortnose sturgeon were determined by visual examination of the gonads using laparoscopy, and were validated by histological examination of gonadal biopsies. 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Relative amount of gonadal fat; gonad size and color; presence of testicular lobes or ovarian lamellae; and color, size and density of oocytes were useful in determining reproductive stage. Gonad morphology of each reproductive stage was similar in Atlantic and shortnose sturgeon. All captive Atlantic sturgeon survived laparoscopy, gained weight at the same rate as unexamined fish and scars from incisions were no longer evident 9–12 months after surgery. Laparoscopic procedures presented here offer a safe and highly reliable way to determine sex and reproductive status for Atlantic and shortnose sturgeon.</p></abstract></profileDesc><revisionDesc><change when="2011-04">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/846A72867B3945DC7967CED861A9E26205B8441C/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>Blackwell Publishing Ltd</publisherName><publisherLoc>Oxford, UK</publisherLoc></publisherInfo><doi origin="wiley" registered="yes">10.1111/(ISSN)1439-0426</doi><issn type="print">0175-8659</issn><issn type="electronic">1439-0426</issn><idGroup><id type="product" value="JAI"></id><id type="publisherDivision" value="ST"></id></idGroup><titleGroup><title type="main" sort="JOURNAL OF APPLIED ICHTHYOLOGY">Journal of Applied Ichthyology</title></titleGroup></publicationMeta><publicationMeta level="part" position="04102"><doi origin="wiley">10.1111/jai.2011.27.issue-2</doi><titleGroup><title type="specialIssueTitle">Proceedings of the 6th International Symposium on Sturgeon Wuhan, China October 25‐31, 2009</title></titleGroup><numberingGroup><numbering type="journalVolume" number="27">27</numbering><numbering type="journalIssue">2</numbering></numberingGroup><coverDate startDate="2011-04">April 2011</coverDate></publicationMeta><publicationMeta level="unit" type="article" position="78" status="forIssue"><doi origin="wiley">10.1111/j.1439-0426.2011.01679.x</doi><idGroup><id type="unit" value="JAI1679"></id></idGroup><countGroup><count type="pageTotal" number="10"></count></countGroup><titleGroup><title type="tocHeading1">Aquaculture</title><title type="tocHeading2"><i>Sorting (Laparascopy, ultrasound)</i></title></titleGroup><copyright>© 2011 Blackwell Verlag, Berlin</copyright><eventGroup><event type="xmlConverted" agent="Converter:BPG_TO_WML3G version:2.4.8 mode:FullText" date="2011-04-12"></event><event type="publishedOnlineFinalForm" date="2011-03-28"></event><event type="firstOnline" date="2011-03-28"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:3.8.8" date="2014-01-28"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-10-24"></event></eventGroup><numberingGroup><numbering type="pageFirst" number="627">627</numbering><numbering type="pageLast" number="636">636</numbering></numberingGroup><correspondenceTo><b>Author’s address:</b> Mark A. Matsche, Cooperative Oxford Laboratory, 904 South Morris Street, Oxford, MD 21654, USA.
E‐mail: <email>mmatsche@dnr.state.md.us</email></correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:JAI.JAI1679.pdf"></link></linkGroup></publicationMeta><contentMeta><unparsedEditorialHistory>Received: July 19, 2010 Accepted: January 10, 2011</unparsedEditorialHistory><countGroup><count type="figureTotal" number="10"></count><count type="tableTotal" number="1"></count></countGroup><titleGroup><title type="main">Use of laparoscopy to determine sex and reproductive status of shortnose sturgeon (<i>Acipenser brevirostrum</i>) and Atlantic sturgeon (<i>Acipenser oxyrinchus oxyrinchus</i>)</title><title type="shortAuthors">M. A. Matsche, R. S. Bakal and K. M. Rosemary</title><title type="short">Laparoscopy to determine sex and reproductive status of sturgeons</title></titleGroup><creators><creator creatorRole="author" xml:id="cr1" affiliationRef="#a1"><personName><givenNames>M. A.</givenNames><familyName>Matsche</familyName></personName></creator><creator creatorRole="author" xml:id="cr2" affiliationRef="#a2"><personName><givenNames>R. S.</givenNames><familyName>Bakal</familyName></personName></creator><creator creatorRole="author" xml:id="cr3" affiliationRef="#a1"><personName><givenNames>K. M.</givenNames><familyName>Rosemary</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="a1" countryCode="US"><unparsedAffiliation>Cooperative Oxford Laboratory, Oxford, MD, USA</unparsedAffiliation></affiliation><affiliation xml:id="a2" countryCode="US"><unparsedAffiliation>United States Fish and Wildlife Service, Raleigh, NC, USA</unparsedAffiliation></affiliation></affiliationGroup><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Summary</title><p>Sex and reproductive maturity of Atlantic and shortnose sturgeon were determined by visual examination of the gonads using laparoscopy, and were validated by histological examination of gonadal biopsies. Surgical anesthesia was induced in all fish with 250 mg L<sup>−1</sup> tricaine methanesulfonate (MS‐222) and maintained throughout procedures with 85 mg L<sup>−1</sup> MS‐222 on a mobile surgical cart. A pair of Ternamian EndoTip cannulae installed through the ventral body wall in each fish, allowed access for a 5‐mm rigid laparoscope and biopsy forceps. Video endocamera use with the laparoscope, following air insufflation of the coelom, provided detailed, high quality imagery to aspirate the swim bladder, examine the gonad and collect biopsies without inducing iatrogenic trauma. Germinal tissue of all immature males, 25% of immature female shortnose sturgeon and 45% of immature female Atlantic sturgeon were concealed by fat preventing sex determination by visual assessment. Morphological features of gonads were used to determine sex in all remaining fish and were 100% in concordance with histological findings. Relative amount of gonadal fat; gonad size and color; presence of testicular lobes or ovarian lamellae; and color, size and density of oocytes were useful in determining reproductive stage. Gonad morphology of each reproductive stage was similar in Atlantic and shortnose sturgeon. All captive Atlantic sturgeon survived laparoscopy, gained weight at the same rate as unexamined fish and scars from incisions were no longer evident 9–12 months after surgery. Laparoscopic procedures presented here offer a safe and highly reliable way to determine sex and reproductive status for Atlantic and shortnose sturgeon.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Use of laparoscopy to determine sex and reproductive status of shortnose sturgeon (Acipenser brevirostrum) and Atlantic sturgeon (Acipenser oxyrinchus oxyrinchus)</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>Laparoscopy to determine sex and reproductive status of sturgeons</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Use of laparoscopy to determine sex and reproductive status of shortnose sturgeon (Acipenser brevirostrum) and Atlantic sturgeon (Acipenser oxyrinchus oxyrinchus)</title></titleInfo><name type="personal"><namePart type="given">M. 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Surgical anesthesia was induced in all fish with 250 mg L−1 tricaine methanesulfonate (MS‐222) and maintained throughout procedures with 85 mg L−1 MS‐222 on a mobile surgical cart. A pair of Ternamian EndoTip cannulae installed through the ventral body wall in each fish, allowed access for a 5‐mm rigid laparoscope and biopsy forceps. Video endocamera use with the laparoscope, following air insufflation of the coelom, provided detailed, high quality imagery to aspirate the swim bladder, examine the gonad and collect biopsies without inducing iatrogenic trauma. Germinal tissue of all immature males, 25% of immature female shortnose sturgeon and 45% of immature female Atlantic sturgeon were concealed by fat preventing sex determination by visual assessment. Morphological features of gonads were used to determine sex in all remaining fish and were 100% in concordance with histological findings. Relative amount of gonadal fat; gonad size and color; presence of testicular lobes or ovarian lamellae; and color, size and density of oocytes were useful in determining reproductive stage. Gonad morphology of each reproductive stage was similar in Atlantic and shortnose sturgeon. All captive Atlantic sturgeon survived laparoscopy, gained weight at the same rate as unexamined fish and scars from incisions were no longer evident 9–12 months after surgery. Laparoscopic procedures presented here offer a safe and highly reliable way to determine sex and reproductive status for Atlantic and shortnose sturgeon.</abstract><relatedItem type="host"><titleInfo><title>Journal of Applied Ichthyology</title></titleInfo><genre type="journal">journal</genre><identifier type="ISSN">0175-8659</identifier><identifier type="eISSN">1439-0426</identifier><identifier type="DOI">10.1111/(ISSN)1439-0426</identifier><identifier type="PublisherID">JAI</identifier><part><date>2011</date><detail type="title"><title>Proceedings of the 6th International Symposium on Sturgeon Wuhan, China October 25‐31, 2009</title></detail><detail type="volume"><caption>vol.</caption><number>27</number></detail><detail type="issue"><caption>no.</caption><number>2</number></detail><extent unit="pages"><start>627</start><end>636</end><total>10</total></extent></part></relatedItem><identifier type="istex">846A72867B3945DC7967CED861A9E26205B8441C</identifier><identifier type="DOI">10.1111/j.1439-0426.2011.01679.x</identifier><identifier type="ArticleID">JAI1679</identifier><accessCondition type="use and reproduction" contentType="copyright">© 2011 Blackwell Verlag, Berlin</accessCondition><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>Blackwell Publishing Ltd</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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