Histochemistry of the development of the digestive system of Siberian sturgeon during early ontogeny
Identifieur interne : 001265 ( Istex/Corpus ); précédent : 001264; suivant : 001266Histochemistry of the development of the digestive system of Siberian sturgeon during early ontogeny
Auteurs : E. Gisbert ; M. C. Sarasquete ; P. Williot ; F. Castell RvaySource :
- Journal of Fish Biology [ 0022-1112 ] ; 1999-09.
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- KwdEn :
Abstract
At hatching, the yolk‐sac matrix of Siberian sturgeon Acipenser baeri contained neutral glycoconjugates, glycogen, proteins rich in arginine, lysine, tyrosine, cysteine and cystine, glycoproteins containing mannose (Man) and/or glucose (Glc), N‐acetyl‐D‐galactosamine (GalNAc), L‐fucose (Fuc), sialic acid and/or N‐acetyl‐D‐glucosamine (GlcNAc) residues, as well as neutral and acidic lipids. Buccopharyngeal and anterior oesophageal goblet cellls produced a combination of neutral and acid sialoglycoproteins, while those from the posterior oesophagus secreted only neutral glycoproteins; both types of secretions contained tryptophan and ‐S‐S‐ groups and were unreactive to lectin techniques. Most intestinal goblet cells secreted mainly carboxylated and sulphated sialoglycoproteins with some rests of neutral glycoconjugates, while few of them produced only acid or neutral glycoproteins. Intestinal glycoproteins were rich in GalNAc, GlcNAc and sialic acid residues. Close relationships between digestive enzymes and morphological development of digestive organs were observed. Histochemistry of enzymes revealed that just after hatching, alkaline and acid phosphatase, ATP‐ase and non‐specific esterase activities were detected in the yolk sac. From the onset of exogenous feeding to the juvenile stage (30 days post‐hatch), an enhancement of enzymatic activities was observed, as alkaline and acid phosphatase, ATP‐ase, aminopeptidase M and nonspecific esterase sharply increased. However, lipase activity decreased in the liver and brush border of enterocytes by 13–14 days post‐hatch. Two types of lipase were detected in the alimentary canal, a non‐pancreatic lipase that was secreted in the cardiac stomach by gastric glands, and a pancreatic lipase, which activity was mainly detected in the brush border of the intestinal epithelium.
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DOI: 10.1111/j.1095-8649.1999.tb00702.x
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ISTEX:6DDEE7063A66BBE7CB56E74C27B166A0525C6337Le document en format XML
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Buccopharyngeal and anterior oesophageal goblet cellls produced a combination of neutral and acid sialoglycoproteins, while those from the posterior oesophagus secreted only neutral glycoproteins; both types of secretions contained tryptophan and ‐S‐S‐ groups and were unreactive to lectin techniques. Most intestinal goblet cells secreted mainly carboxylated and sulphated sialoglycoproteins with some rests of neutral glycoconjugates, while few of them produced only acid or neutral glycoproteins. Intestinal glycoproteins were rich in GalNAc, GlcNAc and sialic acid residues. Close relationships between digestive enzymes and morphological development of digestive organs were observed. Histochemistry of enzymes revealed that just after hatching, alkaline and acid phosphatase, ATP‐ase and non‐specific esterase activities were detected in the yolk sac. From the onset of exogenous feeding to the juvenile stage (30 days post‐hatch), an enhancement of enzymatic activities was observed, as alkaline and acid phosphatase, ATP‐ase, aminopeptidase M and nonspecific esterase sharply increased. However, lipase activity decreased in the liver and brush border of enterocytes by 13–14 days post‐hatch. Two types of lipase were detected in the alimentary canal, a non‐pancreatic lipase that was secreted in the cardiac stomach by gastric glands, and a pancreatic lipase, which activity was mainly detected in the brush border of the intestinal epithelium.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>E. Gisbert</name><affiliations><json:string>Laboratorio de Acuicultura, Depto. Biología Animal, Facultad de Biología, Universitat de Barcelona, Av. Diagonal 645, 08028 Barcelona, Spain</json:string><json:string>E-mail: gisbert@bio.ub.es</json:string></affiliations></json:item><json:item><name>M. C. 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Buccopharyngeal and anterior oesophageal goblet cellls produced a combination of neutral and acid sialoglycoproteins, while those from the posterior oesophagus secreted only neutral glycoproteins; both types of secretions contained tryptophan and ‐S‐S‐ groups and were unreactive to lectin techniques. Most intestinal goblet cells secreted mainly carboxylated and sulphated sialoglycoproteins with some rests of neutral glycoconjugates, while few of them produced only acid or neutral glycoproteins. Intestinal glycoproteins were rich in GalNAc, GlcNAc and sialic acid residues. Close relationships between digestive enzymes and morphological development of digestive organs were observed. Histochemistry of enzymes revealed that just after hatching, alkaline and acid phosphatase, ATP‐ase and non‐specific esterase activities were detected in the yolk sac. From the onset of exogenous feeding to the juvenile stage (30 days post‐hatch), an enhancement of enzymatic activities was observed, as alkaline and acid phosphatase, ATP‐ase, aminopeptidase M and nonspecific esterase sharply increased. However, lipase activity decreased in the liver and brush border of enterocytes by 13–14 days post‐hatch. 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Buccopharyngeal and anterior oesophageal goblet cellls produced a combination of neutral and acid sialoglycoproteins, while those from the posterior oesophagus secreted only neutral glycoproteins; both types of secretions contained tryptophan and ‐S‐S‐ groups and were unreactive to lectin techniques. Most intestinal goblet cells secreted mainly carboxylated and sulphated sialoglycoproteins with some rests of neutral glycoconjugates, while few of them produced only acid or neutral glycoproteins. Intestinal glycoproteins were rich in GalNAc, GlcNAc and sialic acid residues. Close relationships between digestive enzymes and morphological development of digestive organs were observed. Histochemistry of enzymes revealed that just after hatching, alkaline and acid phosphatase, ATP‐ase and non‐specific esterase activities were detected in the yolk sac. From the onset of exogenous feeding to the juvenile stage (30 days post‐hatch), an enhancement of enzymatic activities was observed, as alkaline and acid phosphatase, ATP‐ase, aminopeptidase M and nonspecific esterase sharply increased. However, lipase activity decreased in the liver and brush border of enterocytes by 13–14 days post‐hatch. Two types of lipase were detected in the alimentary canal, a non‐pancreatic lipase that was secreted in the cardiac stomach by gastric glands, and a pancreatic lipase, which activity was mainly detected in the brush border of the intestinal epithelium.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>keywords</head><item><term>digestive system</term></item><item><term>histochemistry</term></item><item><term>enzymes</term></item><item><term>Chondrostei</term></item><item><term>larvae</term></item><item><term>juveniles</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1999-09">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/6DDEE7063A66BBE7CB56E74C27B166A0525C6337/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>Blackwell Publishing Ltd</publisherName><publisherLoc>Oxford, UK</publisherLoc></publisherInfo><doi origin="wiley" registered="yes">10.1111/(ISSN)1095-8649</doi><issn type="print">0022-1112</issn><issn type="electronic">1095-8649</issn><idGroup><id type="product" value="JFB"></id><id type="publisherDivision" value="ST"></id></idGroup><titleGroup><title type="main" sort="JOURNAL OF FISH BIOLOGY">Journal of Fish Biology</title></titleGroup></publicationMeta><publicationMeta level="part" position="09003"><doi origin="wiley">10.1111/jfb.1999.55.issue-3</doi><numberingGroup><numbering type="journalVolume" number="55">55</numbering><numbering type="journalIssue" number="3">3</numbering></numberingGroup><coverDate startDate="1999-09">September 1999</coverDate></publicationMeta><publicationMeta level="unit" type="article" position="0059600" status="forIssue"><doi origin="wiley">10.1111/j.1095-8649.1999.tb00702.x</doi><idGroup><id type="unit" value="JFB596"></id></idGroup><countGroup><count type="pageTotal" number="21"></count></countGroup><eventGroup><event type="firstOnline" date="2005-04-01"></event><event type="publishedOnlineFinalForm" date="2005-04-01"></event><event type="xmlConverted" agent="Converter:BPG_TO_WML3G version:2.3.6 mode:FullText source:HeaderRef result:HeaderRef" date="2010-04-20"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:3.8.8" date="2014-01-30"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-10-30"></event></eventGroup><numberingGroup><numbering type="pageFirst" number="596">596</numbering><numbering type="pageLast" number="616">616</numbering></numberingGroup><correspondenceTo> Author to whom correspondence should be addressed. Tel.: +343 4021447; fax: +343 4035740; email: <email>gisbert@bio.ub.es</email></correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:JFB.JFB596.pdf"></link></linkGroup></publicationMeta><contentMeta><unparsedEditorialHistory>(Received 24 December 1998, Accepted 1 June 1999)</unparsedEditorialHistory><countGroup><count type="referenceTotal" number="45"></count><count type="linksCrossRef" number="5"></count></countGroup><titleGroup><title type="main">Histochemistry of the development of the digestive system of Siberian sturgeon during early ontogeny</title></titleGroup><creators><creator creatorRole="author" xml:id="cr1" affiliationRef="#a1" corresponding="yes"><personName><givenNames>E.</givenNames><familyName>Gisbert</familyName></personName></creator><creator creatorRole="author" xml:id="cr2" affiliationRef="#a2"><personName><givenNames>M. C.</givenNames><familyName>Sarasquete</familyName></personName></creator><creator creatorRole="author" xml:id="cr3" affiliationRef="#a3"><personName><givenNames>P.</givenNames><familyName>Williot</familyName></personName></creator><creator creatorRole="author" xml:id="cr4" affiliationRef="#a1"><personName><givenNames>F.</givenNames><familyName>Castelló‐Orvay</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="a1" countryCode="ES"><unparsedAffiliation>Laboratorio de Acuicultura, Depto. Biología Animal, Facultad de Biología, Universitat de Barcelona, Av. 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Buccopharyngeal and anterior oesophageal goblet cellls produced a combination of neutral and acid sialoglycoproteins, while those from the posterior oesophagus secreted only neutral glycoproteins; both types of secretions contained tryptophan and ‐S‐S‐ groups and were unreactive to lectin techniques. Most intestinal goblet cells secreted mainly carboxylated and sulphated sialoglycoproteins with some rests of neutral glycoconjugates, while few of them produced only acid or neutral glycoproteins. Intestinal glycoproteins were rich in GalNAc, GlcNAc and sialic acid residues. Close relationships between digestive enzymes and morphological development of digestive organs were observed. Histochemistry of enzymes revealed that just after hatching, alkaline and acid phosphatase, <i>ATP</i>‐ase and non‐specific esterase activities were detected in the yolk sac. From the onset of exogenous feeding to the juvenile stage (30 days post‐hatch), an enhancement of enzymatic activities was observed, as alkaline and acid phosphatase, <i>ATP</i>‐ase, aminopeptidase M and nonspecific esterase sharply increased. However, lipase activity decreased in the liver and brush border of enterocytes by 13–14 days post‐hatch. Two types of lipase were detected in the alimentary canal, a non‐pancreatic lipase that was secreted in the cardiac stomach by gastric glands, and a pancreatic lipase, which activity was mainly detected in the brush border of the intestinal epithelium.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Histochemistry of the development of the digestive system of Siberian sturgeon during early ontogeny</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Histochemistry of the development of the digestive system of Siberian sturgeon during early ontogeny</title></titleInfo><name type="personal"><namePart type="given">E.</namePart><namePart type="family">Gisbert</namePart><affiliation>Laboratorio de Acuicultura, Depto. Biología Animal, Facultad de Biología, Universitat de Barcelona, Av. Diagonal 645, 08028 Barcelona, Spain</affiliation><affiliation>E-mail: gisbert@bio.ub.es</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M. C.</namePart><namePart type="family">Sarasquete</namePart><affiliation>Instituto de Ciencias Marinas de Andalucía (C.S.I.C.), Apartado Oficial, 11510 Puerto Real, Cádiz, Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">P.</namePart><namePart type="family">Williot</namePart><affiliation>Cemagref, Groupement de Bordeaux, 50 avenue de Verdum, B.P. 3, 33611 Gazinet Cedex, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">F.</namePart><namePart type="family">Castelló‐Orvay</namePart><affiliation>Laboratorio de Acuicultura, Depto. Biología Animal, Facultad de Biología, Universitat de Barcelona, Av. Diagonal 645, 08028 Barcelona, Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Blackwell Publishing Ltd</publisher><place><placeTerm type="text">Oxford, UK</placeTerm></place><dateIssued encoding="w3cdtf">1999-09</dateIssued><edition>(Received 24 December 1998, Accepted 1 June 1999)</edition><copyrightDate encoding="w3cdtf">1999</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="references">45</extent></physicalDescription><abstract lang="en">At hatching, the yolk‐sac matrix of Siberian sturgeon Acipenser baeri contained neutral glycoconjugates, glycogen, proteins rich in arginine, lysine, tyrosine, cysteine and cystine, glycoproteins containing mannose (Man) and/or glucose (Glc), N‐acetyl‐D‐galactosamine (GalNAc), L‐fucose (Fuc), sialic acid and/or N‐acetyl‐D‐glucosamine (GlcNAc) residues, as well as neutral and acidic lipids. Buccopharyngeal and anterior oesophageal goblet cellls produced a combination of neutral and acid sialoglycoproteins, while those from the posterior oesophagus secreted only neutral glycoproteins; both types of secretions contained tryptophan and ‐S‐S‐ groups and were unreactive to lectin techniques. Most intestinal goblet cells secreted mainly carboxylated and sulphated sialoglycoproteins with some rests of neutral glycoconjugates, while few of them produced only acid or neutral glycoproteins. Intestinal glycoproteins were rich in GalNAc, GlcNAc and sialic acid residues. Close relationships between digestive enzymes and morphological development of digestive organs were observed. Histochemistry of enzymes revealed that just after hatching, alkaline and acid phosphatase, ATP‐ase and non‐specific esterase activities were detected in the yolk sac. From the onset of exogenous feeding to the juvenile stage (30 days post‐hatch), an enhancement of enzymatic activities was observed, as alkaline and acid phosphatase, ATP‐ase, aminopeptidase M and nonspecific esterase sharply increased. However, lipase activity decreased in the liver and brush border of enterocytes by 13–14 days post‐hatch. Two types of lipase were detected in the alimentary canal, a non‐pancreatic lipase that was secreted in the cardiac stomach by gastric glands, and a pancreatic lipase, which activity was mainly detected in the brush border of the intestinal epithelium.</abstract><subject lang="en"><genre>keywords</genre><topic>digestive system</topic><topic>histochemistry</topic><topic>enzymes</topic><topic>Chondrostei</topic><topic>larvae</topic><topic>juveniles</topic></subject><relatedItem type="host"><titleInfo><title>Journal of Fish Biology</title></titleInfo><genre type="journal">journal</genre><identifier type="ISSN">0022-1112</identifier><identifier type="eISSN">1095-8649</identifier><identifier type="DOI">10.1111/(ISSN)1095-8649</identifier><identifier type="PublisherID">JFB</identifier><part><date>1999</date><detail type="volume"><caption>vol.</caption><number>55</number></detail><detail type="issue"><caption>no.</caption><number>3</number></detail><extent unit="pages"><start>596</start><end>616</end><total>21</total></extent></part></relatedItem><identifier type="istex">6DDEE7063A66BBE7CB56E74C27B166A0525C6337</identifier><identifier type="DOI">10.1111/j.1095-8649.1999.tb00702.x</identifier><identifier type="ArticleID">JFB596</identifier><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>Blackwell Publishing Ltd</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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