Studies on the semen biology and sperm cryopreservation in the sterlet, Acipenser ruthenus L.
Identifieur interne : 001225 ( Istex/Corpus ); précédent : 001224; suivant : 001226Studies on the semen biology and sperm cryopreservation in the sterlet, Acipenser ruthenus L.
Auteurs : F. Lahnsteiner ; B. Berger ; A. Horvath ; B. UrbanyiSource :
- Aquaculture Research [ 1355-557X ] ; 2004-05.
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Abstract
The present study investigated motility, acrosome reaction, fertility and cryobiological parameters of the semen of the sterlet, Acipenser ruthenus L. Sperm motility persisted for about 4 min in water, and the main swimming type was the linear motion. Motility was prolonged at osmolalities of 12.5 mosmol kg−1 and in the presence of magnesium ions, while calcium had no effect. Also a pH in the range of 7.0–9.0 had no effect on ` motility. At osmolalities of 25–50 mosmol kg−1 the sperm motility was partly inhibited, at osmolalities of 100 mosmol kg−1, completely and irreversibly. In 50 mosmol kg−1 solutions with 2.5–5 mM L−1 KCl the motility inhibition was total, but reversible. The acrosome reaction was not induced by one of the described solutions, but the percentage of spermatozoa with reacted acrosomes was low (<20%) and highly variable in all experiments. The optimal extender base for cryopreservation was a solution consisting of 50 mM L−1 NaCl, 5 mM L−1 KCl, 10 mM L−1 Tris (pH 8.5). From the tested cryoprotectants only dimethylsulphoxide (DMSO) and methanol provided sufficient cryoprotection. After freezing and thawing, the motility rates and swimming velocities were higher with DMSO than with methanol. However, the fertility was very significantly reduced with DMSO (10.3±0.5%) while with methanol fertilization rates in a similar range (32.7±4.4%) as with fresh semen (33.90±0.8%) could be obtained. Optimal freezing conditions for sterlet semen were in the vapour of liquid nitrogen 3–5 cm (−95°C to −85°C) above its surface, the optimal thawing conditions at 25°C for 30 s. The acrosome reaction was not induced by these cryopreservation protocols.
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DOI: 10.1111/j.1365-2109.2004.01034.x
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Sperm motility persisted for about 4 min in water, and the main swimming type was the linear motion. Motility was prolonged at osmolalities of 12.5 mosmol kg−1 and in the presence of magnesium ions, while calcium had no effect. Also a pH in the range of 7.0–9.0 had no effect on ` motility. At osmolalities of 25–50 mosmol kg−1 the sperm motility was partly inhibited, at osmolalities of 100 mosmol kg−1, completely and irreversibly. In 50 mosmol kg−1 solutions with 2.5–5 mM L−1 KCl the motility inhibition was total, but reversible. The acrosome reaction was not induced by one of the described solutions, but the percentage of spermatozoa with reacted acrosomes was low (<20%) and highly variable in all experiments. The optimal extender base for cryopreservation was a solution consisting of 50 mM L−1 NaCl, 5 mM L−1 KCl, 10 mM L−1 Tris (pH 8.5). From the tested cryoprotectants only dimethylsulphoxide (DMSO) and methanol provided sufficient cryoprotection. After freezing and thawing, the motility rates and swimming velocities were higher with DMSO than with methanol. However, the fertility was very significantly reduced with DMSO (10.3±0.5%) while with methanol fertilization rates in a similar range (32.7±4.4%) as with fresh semen (33.90±0.8%) could be obtained. Optimal freezing conditions for sterlet semen were in the vapour of liquid nitrogen 3–5 cm (−95°C to −85°C) above its surface, the optimal thawing conditions at 25°C for 30 s. 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Sperm motility persisted for about 4 min in water, and the main swimming type was the linear motion. Motility was prolonged at osmolalities of 12.5 mosmol kg−1 and in the presence of magnesium ions, while calcium had no effect. Also a pH in the range of 7.0–9.0 had no effect on ` motility. At osmolalities of 25–50 mosmol kg−1 the sperm motility was partly inhibited, at osmolalities of 100 mosmol kg−1, completely and irreversibly. In 50 mosmol kg−1 solutions with 2.5–5 mM L−1 KCl the motility inhibition was total, but reversible. The acrosome reaction was not induced by one of the described solutions, but the percentage of spermatozoa with reacted acrosomes was low (<20%) and highly variable in all experiments. The optimal extender base for cryopreservation was a solution consisting of 50 mM L−1 NaCl, 5 mM L−1 KCl, 10 mM L−1 Tris (pH 8.5). From the tested cryoprotectants only dimethylsulphoxide (DMSO) and methanol provided sufficient cryoprotection. After freezing and thawing, the motility rates and swimming velocities were higher with DMSO than with methanol. However, the fertility was very significantly reduced with DMSO (10.3±0.5%) while with methanol fertilization rates in a similar range (32.7±4.4%) as with fresh semen (33.90±0.8%) could be obtained. Optimal freezing conditions for sterlet semen were in the vapour of liquid nitrogen 3–5 cm (−95°C to −85°C) above its surface, the optimal thawing conditions at 25°C for 30 s. The acrosome reaction was not induced by these cryopreservation protocols.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>keywords</head><item><term>spermatozoa</term></item><item><term>motility</term></item><item><term>acrosome</term></item><item><term>cryopreservation</term></item><item><term>sterlet</term></item><item><term>Acipenser ruthenus</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2004-05">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/34AE4679CF3B2774FCF4CAABD9C26B16A0126EF9/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>Blackwell Science Ltd</publisherName><publisherLoc>Oxford, UK</publisherLoc></publisherInfo><doi origin="wiley" registered="yes">10.1111/(ISSN)1365-2109</doi><issn type="print">1355-557X</issn><issn type="electronic">1365-2109</issn><idGroup><id type="product" value="ARE"></id><id type="publisherDivision" value="ST"></id></idGroup><titleGroup><title type="main" sort="AQUACULTURE RESEARCH">Aquaculture Research</title></titleGroup></publicationMeta><publicationMeta level="part" position="05006"><doi origin="wiley">10.1111/are.2004.35.issue-6</doi><numberingGroup><numbering type="journalVolume" number="35">35</numbering><numbering type="journalIssue" number="6">6</numbering></numberingGroup><coverDate startDate="2004-05">May 2004</coverDate></publicationMeta><publicationMeta level="unit" type="article" position="1" status="forIssue"><doi origin="wiley">10.1111/j.1365-2109.2004.01034.x</doi><idGroup><id type="unit" value="ARE1034"></id><id type="supplier" value="1034"></id></idGroup><countGroup><count type="pageTotal" number="10"></count></countGroup><titleGroup><title type="tocHeading1">Original articles</title></titleGroup><eventGroup><event type="firstOnline" date="2004-04-23"></event><event type="publishedOnlineFinalForm" date="2004-04-23"></event><event type="xmlConverted" agent="Converter:BPG_TO_WML3G version:2.3.4 mode:FullText source:FullText result:FullText" date="2010-03-30"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:3.8.8" date="2014-01-06"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-10-15"></event></eventGroup><numberingGroup><numbering type="pageFirst" number="519">519</numbering><numbering type="pageLast" number="528">528</numbering></numberingGroup><correspondenceTo><b>Correspondence:</b> Dr F Lahnsteiner, Institute for Zoology, University of Salzburg, Hellbrunnerstrasse 34, A‐5020 Salzburg, Austria. E‐mail: <email normalForm="Franz.Lahnsteiner@sbg.ac.at">Franz.Lahnsteiner@sbg.ac.at</email></correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:ARE.ARE1034.pdf"></link></linkGroup></publicationMeta><contentMeta><countGroup><count type="figureTotal" number="0"></count><count type="tableTotal" number="8"></count><count type="formulaTotal" number="0"></count><count type="referenceTotal" number="18"></count><count type="wordTotal" number="7244"></count><count type="linksCrossRef" number="63"></count></countGroup><titleGroup><title type="main">Studies on the semen biology and sperm cryopreservation in the sterlet, <i>Acipenser ruthenus</i> L.</title><title type="shortAuthors">F Lahnsteiner et al.</title><title type="short">Semen biology and sperm cryopreservation in the sterlet</title></titleGroup><creators><creator creatorRole="author" xml:id="cr1" affiliationRef="#a1"><personName><givenNames>F</givenNames><familyName>Lahnsteiner</familyName></personName></creator><creator creatorRole="author" xml:id="cr2" affiliationRef="#a2"><personName><givenNames>B</givenNames><familyName>Berger</familyName></personName></creator><creator creatorRole="author" xml:id="cr3" affiliationRef="#a3"><personName><givenNames>A</givenNames><familyName>Horvath</familyName></personName></creator><creator creatorRole="author" xml:id="cr4" affiliationRef="#a3"><personName><givenNames>B</givenNames><familyName>Urbanyi</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="a1" countryCode="AT"><unparsedAffiliation>Institute for Zoology, University of Salzburg, Salzburg, Austria</unparsedAffiliation></affiliation><affiliation xml:id="a2" countryCode="AT"><unparsedAffiliation>Institut für biologische Landwirtschaft und Biodiversität, Austrasse, Thalheim, Austria</unparsedAffiliation></affiliation><affiliation xml:id="a3" countryCode="HU"><unparsedAffiliation>Laboratory of Fish Culture, Institute of Animal Husbandry, Department of Applied Animal Genetics and Breeding, Gödöllö University of Agricultural Sciences, Hungary</unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en"><keyword xml:id="k1">spermatozoa</keyword><keyword xml:id="k2">motility</keyword><keyword xml:id="k3">acrosome</keyword><keyword xml:id="k4">cryopreservation</keyword><keyword xml:id="k5">sterlet</keyword><keyword xml:id="k6"><i>Acipenser ruthenus</i></keyword></keywordGroup><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Abstract</title><p>The present study investigated motility, acrosome reaction, fertility and cryobiological parameters of the semen of the sterlet, <i>Acipenser ruthenu</i>s L. Sperm motility persisted for about 4 min in water, and the main swimming type was the linear motion. Motility was prolonged at osmolalities of 12.5 mosmol kg<sup>−1</sup> and in the presence of magnesium ions, while calcium had no effect. Also a pH in the range of 7.0–9.0 had no effect on ` motility. At osmolalities of 25–50 mosmol kg<sup>−1</sup> the sperm motility was partly inhibited, at osmolalities of 100 mosmol kg<sup>−1</sup>, completely and irreversibly. In 50 mosmol kg<sup>−1</sup> solutions with 2.5–5 mM L<sup>−1</sup> KCl the motility inhibition was total, but reversible. The acrosome reaction was not induced by one of the described solutions, but the percentage of spermatozoa with reacted acrosomes was low (<20%) and highly variable in all experiments. The optimal extender base for cryopreservation was a solution consisting of 50 mM L<sup>−1</sup> NaCl, 5 mM L<sup>−1</sup> KCl, 10 mM L<sup>−1</sup> Tris (pH 8.5). From the tested cryoprotectants only dimethylsulphoxide (DMSO) and methanol provided sufficient cryoprotection. After freezing and thawing, the motility rates and swimming velocities were higher with DMSO than with methanol. However, the fertility was very significantly reduced with DMSO (10.3±0.5%) while with methanol fertilization rates in a similar range (32.7±4.4%) as with fresh semen (33.90±0.8%) could be obtained. Optimal freezing conditions for sterlet semen were in the vapour of liquid nitrogen 3–5 cm (−95°C to −85°C) above its surface, the optimal thawing conditions at 25°C for 30 s. The acrosome reaction was not induced by these cryopreservation protocols.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Studies on the semen biology and sperm cryopreservation in the sterlet, Acipenser ruthenus L.</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>Semen biology and sperm cryopreservation in the sterlet</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Studies on the semen biology and sperm cryopreservation in the sterlet, Acipenser ruthenus L.</title></titleInfo><name type="personal"><namePart type="given">F</namePart><namePart type="family">Lahnsteiner</namePart><affiliation>Institute for Zoology, University of Salzburg, Salzburg, Austria</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">B</namePart><namePart type="family">Berger</namePart><affiliation>Institut für biologische Landwirtschaft und Biodiversität, Austrasse, Thalheim, Austria</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">A</namePart><namePart type="family">Horvath</namePart><affiliation>Laboratory of Fish Culture, Institute of Animal Husbandry, Department of Applied Animal Genetics and Breeding, Gödöllö University of Agricultural Sciences, Hungary</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">B</namePart><namePart type="family">Urbanyi</namePart><affiliation>Laboratory of Fish Culture, Institute of Animal Husbandry, Department of Applied Animal Genetics and Breeding, Gödöllö University of Agricultural Sciences, Hungary</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Blackwell Science Ltd</publisher><place><placeTerm type="text">Oxford, UK</placeTerm></place><dateIssued encoding="w3cdtf">2004-05</dateIssued><copyrightDate encoding="w3cdtf">2004</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="tables">8</extent><extent unit="references">18</extent><extent unit="words">7244</extent></physicalDescription><abstract lang="en">The present study investigated motility, acrosome reaction, fertility and cryobiological parameters of the semen of the sterlet, Acipenser ruthenus L. Sperm motility persisted for about 4 min in water, and the main swimming type was the linear motion. Motility was prolonged at osmolalities of 12.5 mosmol kg−1 and in the presence of magnesium ions, while calcium had no effect. Also a pH in the range of 7.0–9.0 had no effect on ` motility. At osmolalities of 25–50 mosmol kg−1 the sperm motility was partly inhibited, at osmolalities of 100 mosmol kg−1, completely and irreversibly. In 50 mosmol kg−1 solutions with 2.5–5 mM L−1 KCl the motility inhibition was total, but reversible. The acrosome reaction was not induced by one of the described solutions, but the percentage of spermatozoa with reacted acrosomes was low (<20%) and highly variable in all experiments. The optimal extender base for cryopreservation was a solution consisting of 50 mM L−1 NaCl, 5 mM L−1 KCl, 10 mM L−1 Tris (pH 8.5). From the tested cryoprotectants only dimethylsulphoxide (DMSO) and methanol provided sufficient cryoprotection. After freezing and thawing, the motility rates and swimming velocities were higher with DMSO than with methanol. However, the fertility was very significantly reduced with DMSO (10.3±0.5%) while with methanol fertilization rates in a similar range (32.7±4.4%) as with fresh semen (33.90±0.8%) could be obtained. Optimal freezing conditions for sterlet semen were in the vapour of liquid nitrogen 3–5 cm (−95°C to −85°C) above its surface, the optimal thawing conditions at 25°C for 30 s. The acrosome reaction was not induced by these cryopreservation protocols.</abstract><subject lang="en"><genre>keywords</genre><topic>spermatozoa</topic><topic>motility</topic><topic>acrosome</topic><topic>cryopreservation</topic><topic>sterlet</topic><topic>Acipenser ruthenus</topic></subject><relatedItem type="host"><titleInfo><title>Aquaculture Research</title></titleInfo><genre type="journal">journal</genre><identifier type="ISSN">1355-557X</identifier><identifier type="eISSN">1365-2109</identifier><identifier type="DOI">10.1111/(ISSN)1365-2109</identifier><identifier type="PublisherID">ARE</identifier><part><date>2004</date><detail type="volume"><caption>vol.</caption><number>35</number></detail><detail type="issue"><caption>no.</caption><number>6</number></detail><extent unit="pages"><start>519</start><end>528</end><total>10</total></extent></part></relatedItem><identifier type="istex">34AE4679CF3B2774FCF4CAABD9C26B16A0126EF9</identifier><identifier type="DOI">10.1111/j.1365-2109.2004.01034.x</identifier><identifier type="ArticleID">ARE1034</identifier><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>Blackwell Science Ltd</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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