Proteolytic Activity in California Halibut Larvae (Paralichthys californicus)
Identifieur interne : 001181 ( Istex/Corpus ); précédent : 001180; suivant : 001182Proteolytic Activity in California Halibut Larvae (Paralichthys californicus)
Auteurs : Magali Zacarias-Soto ; Jean B. Muguet ; Juan P. LazoSource :
- Journal of the World Aquaculture Society [ 0893-8849 ] ; 2006-06.
Abstract
The digestive tract of many marine fish larvae undergoes numerous morphological and functional changes during ontogeny that can substantially influence larval survival under culture conditions. Increasing our knowledge of the digestive capacity and nutritional requirements of the larvae of new candidate species for aquaculture will aid in the development of optimal feeding protocols and greatly improve production under hatchery conditions. In this study, we assess the proteolytic capacity of California halibut (Paralichthys californicus) larvae using biochemical and histological analyses. Newly hatched larvae were reared in a semiclosed recirculating system and fed with highly unsaturated fatty acid (HUFA)–enriched rotifers from hatching until 19 d posthatch (dph) and HUFA‐enriched Artemia nauplii thereafter. Total and specific activity of trypsin and leucine‐aminopeptidase (LAP) and acid and alkaline protease activities were assessed throughout development using spectrophotometric techniques. Trypsin‐like activity and LAP and alkaline protease activities were detected shortly after hatching and before the opening of the mouth. Acid protease activity was not detected until 36–40 dph, concomitant with the development of the gastric glands. The specific activity of trypsin and LAP showed two distinct peaks at 8 and 20 dph. The second peak coincided with the shift from rotifers to Artemia. Hence, newly hatched California halibut larvae possess alkaline proteolytic activity before first feeding. Based on the digestive capacity evaluated in this study and the timing of the development of the functional stomach, we propose that California halibut can be adequately weaned to formulated microdiets around 36 dph.
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DOI: 10.1111/j.1749-7345.2006.00024.x
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C.I.C.E.S.E., Km. 107 Carretera Tijuana – Ensenada, Ensenada, Baja California 22860 México</mods:affiliation></affiliation></author><author><name sortKey="Muguet, Jean B" sort="Muguet, Jean B" uniqKey="Muguet J" first="Jean B." last="Muguet">Jean B. Muguet</name><affiliation><mods:affiliation>Department of Animal Science, University of California, Davis, California 95616 USA</mods:affiliation></affiliation></author><author><name sortKey="Lazo, Juan P" sort="Lazo, Juan P" uniqKey="Lazo J" first="Juan P." last="Lazo">Juan P. Lazo</name><affiliation><mods:affiliation>Department of Aquaculture, Center for Scientific Research and Higher Education of Ensenada. C.I.C.E.S.E., Km. 107 Carretera Tijuana – Ensenada, Ensenada, Baja California 22860 México</mods:affiliation></affiliation><affiliation><mods:affiliation>Corresponding author.</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of the World Aquaculture Society</title><idno type="ISSN">0893-8849</idno><idno type="eISSN">1749-7345</idno><imprint><publisher>Blackwell Publishing Inc</publisher><pubPlace>Malden, USA</pubPlace><date type="published" when="2006-06">2006-06</date><biblScope unit="volume">37</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="175">175</biblScope><biblScope unit="page" to="185">185</biblScope></imprint><idno type="ISSN">0893-8849</idno></series><idno type="istex">5CB84C406AF118A9F0E524CA6848FF1893ED1FEE</idno><idno type="DOI">10.1111/j.1749-7345.2006.00024.x</idno><idno type="ArticleID">JWAS24</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0893-8849</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract">The digestive tract of many marine fish larvae undergoes numerous morphological and functional changes during ontogeny that can substantially influence larval survival under culture conditions. Increasing our knowledge of the digestive capacity and nutritional requirements of the larvae of new candidate species for aquaculture will aid in the development of optimal feeding protocols and greatly improve production under hatchery conditions. In this study, we assess the proteolytic capacity of California halibut (Paralichthys californicus) larvae using biochemical and histological analyses. Newly hatched larvae were reared in a semiclosed recirculating system and fed with highly unsaturated fatty acid (HUFA)–enriched rotifers from hatching until 19 d posthatch (dph) and HUFA‐enriched Artemia nauplii thereafter. Total and specific activity of trypsin and leucine‐aminopeptidase (LAP) and acid and alkaline protease activities were assessed throughout development using spectrophotometric techniques. Trypsin‐like activity and LAP and alkaline protease activities were detected shortly after hatching and before the opening of the mouth. Acid protease activity was not detected until 36–40 dph, concomitant with the development of the gastric glands. The specific activity of trypsin and LAP showed two distinct peaks at 8 and 20 dph. The second peak coincided with the shift from rotifers to Artemia. Hence, newly hatched California halibut larvae possess alkaline proteolytic activity before first feeding. Based on the digestive capacity evaluated in this study and the timing of the development of the functional stomach, we propose that California halibut can be adequately weaned to formulated microdiets around 36 dph.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>Magali Zacarias‐Soto</name><affiliations><json:string>Department of Aquaculture, Center for Scientific Research and Higher Education of Ensenada. C.I.C.E.S.E., Km. 107 Carretera Tijuana – Ensenada, Ensenada, Baja California 22860 México</json:string></affiliations></json:item><json:item><name>Jean B. Muguet</name><affiliations><json:string>Department of Animal Science, University of California, Davis, California 95616 USA</json:string></affiliations></json:item><json:item><name>Juan P. Lazo</name><affiliations><json:string>Department of Aquaculture, Center for Scientific Research and Higher Education of Ensenada. C.I.C.E.S.E., Km. 107 Carretera Tijuana – Ensenada, Ensenada, Baja California 22860 México</json:string><json:string>Corresponding author.</json:string></affiliations></json:item></author><articleId><json:string>JWAS24</json:string></articleId><language><json:string>eng</json:string></language><originalGenre><json:string>article</json:string></originalGenre><abstract>The digestive tract of many marine fish larvae undergoes numerous morphological and functional changes during ontogeny that can substantially influence larval survival under culture conditions. Increasing our knowledge of the digestive capacity and nutritional requirements of the larvae of new candidate species for aquaculture will aid in the development of optimal feeding protocols and greatly improve production under hatchery conditions. In this study, we assess the proteolytic capacity of California halibut (Paralichthys californicus) larvae using biochemical and histological analyses. Newly hatched larvae were reared in a semiclosed recirculating system and fed with highly unsaturated fatty acid (HUFA)–enriched rotifers from hatching until 19 d posthatch (dph) and HUFA‐enriched Artemia nauplii thereafter. Total and specific activity of trypsin and leucine‐aminopeptidase (LAP) and acid and alkaline protease activities were assessed throughout development using spectrophotometric techniques. Trypsin‐like activity and LAP and alkaline protease activities were detected shortly after hatching and before the opening of the mouth. Acid protease activity was not detected until 36–40 dph, concomitant with the development of the gastric glands. The specific activity of trypsin and LAP showed two distinct peaks at 8 and 20 dph. The second peak coincided with the shift from rotifers to Artemia. Hence, newly hatched California halibut larvae possess alkaline proteolytic activity before first feeding. 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Acid protease activity was not detected until 36–40 dph, concomitant with the development of the gastric glands. The specific activity of trypsin and LAP showed two distinct peaks at 8 and 20 dph. The second peak coincided with the shift from rotifers to Artemia. Hence, newly hatched California halibut larvae possess alkaline proteolytic activity before first feeding. Based on the digestive capacity evaluated in this study and the timing of the development of the functional stomach, we propose that California halibut can be adequately weaned to formulated microdiets around 36 dph.</p></abstract></profileDesc><revisionDesc><change when="2006-06">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/5CB84C406AF118A9F0E524CA6848FF1893ED1FEE/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>Blackwell Publishing Inc</publisherName><publisherLoc>Malden, USA</publisherLoc></publisherInfo><doi origin="wiley" registered="yes">10.1111/(ISSN)1749-7345</doi><issn type="print">0893-8849</issn><issn type="electronic">1749-7345</issn><idGroup><id type="product" value="JWAS"></id><id type="publisherDivision" value="ST"></id></idGroup><titleGroup><title type="main" sort="JOURNAL OF WORLD AQUACULTURE SOCIETY">Journal of the World Aquaculture Society</title></titleGroup></publicationMeta><publicationMeta level="part" position="06002"><doi origin="wiley">10.1111/jwas.2006.37.issue-2</doi><numberingGroup><numbering type="journalVolume" number="37">37</numbering><numbering type="journalIssue" number="2">2</numbering></numberingGroup><coverDate startDate="2006-06">June 2006</coverDate></publicationMeta><publicationMeta level="unit" type="article" position="5" status="forIssue"><doi origin="wiley">10.1111/j.1749-7345.2006.00024.x</doi><idGroup><id type="unit" value="JWAS24"></id></idGroup><countGroup><count type="pageTotal" number="11"></count></countGroup><titleGroup><title type="tocHeading1">Research Articles</title></titleGroup><eventGroup><event type="firstOnline" date="2006-05-18"></event><event type="publishedOnlineFinalForm" date="2006-05-18"></event><event type="xmlConverted" agent="Converter:BPG_TO_WML3G version:2.3.6 mode:FullText source:Header result:Header" date="2010-04-21"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:3.8.8" date="2014-02-01"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-11-01"></event></eventGroup><numberingGroup><numbering type="pageFirst" number="175">175</numbering><numbering type="pageLast" number="185">185</numbering></numberingGroup><correspondenceTo>Corresponding author.</correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:JWAS.JWAS24.pdf"></link></linkGroup></publicationMeta><contentMeta><countGroup><count type="figureTotal" number="6"></count><count type="tableTotal" number="0"></count><count type="formulaTotal" number="0"></count><count type="referenceTotal" number="46"></count><count type="wordTotal" number="0"></count><count type="linksPubMed" number="0"></count><count type="linksCrossRef" number="0"></count></countGroup><titleGroup><title type="main">Proteolytic Activity in California Halibut Larvae (<i>Paralichthys californicus</i>)</title></titleGroup><creators><creator creatorRole="author" xml:id="cr1" affiliationRef="#a1" noteRef="#fn1"><personName><givenNames>Magali</givenNames><familyName>Zacarias‐Soto</familyName></personName></creator><creator creatorRole="author" xml:id="cr2" affiliationRef="#a2"><personName><givenNames>Jean B.</givenNames><familyName>Muguet</familyName></personName></creator><creator creatorRole="author" xml:id="cr3" affiliationRef="#a3" noteRef="#fn1" corresponding="yes"><personName><givenNames>Juan P.</givenNames><familyName>Lazo</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="a1" countryCode="US"><unparsedAffiliation><i>Department of Aquaculture, Center for Scientific Research and Higher Education of Ensenada. 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Increasing our knowledge of the digestive capacity and nutritional requirements of the larvae of new candidate species for aquaculture will aid in the development of optimal feeding protocols and greatly improve production under hatchery conditions. In this study, we assess the proteolytic capacity of California halibut (<i>Paralichthys californicus</i>) larvae using biochemical and histological analyses. Newly hatched larvae were reared in a semiclosed recirculating system and fed with highly unsaturated fatty acid (HUFA)–enriched rotifers from hatching until 19 d posthatch (dph) and HUFA‐enriched <i>Artemia</i> nauplii thereafter. Total and specific activity of trypsin and leucine‐aminopeptidase (LAP) and acid and alkaline protease activities were assessed throughout development using spectrophotometric techniques. Trypsin‐like activity and LAP and alkaline protease activities were detected shortly after hatching and before the opening of the mouth. Acid protease activity was not detected until 36–40 dph, concomitant with the development of the gastric glands. The specific activity of trypsin and LAP showed two distinct peaks at 8 and 20 dph. The second peak coincided with the shift from rotifers to <i>Artemia</i>. Hence, newly hatched California halibut larvae possess alkaline proteolytic activity before first feeding. Based on the digestive capacity evaluated in this study and the timing of the development of the functional stomach, we propose that California halibut can be adequately weaned to formulated microdiets around 36 dph.</b> </p></abstract></abstractGroup></contentMeta><noteGroup><note xml:id="fn1"><label>1</label><p>Present address: PO Box 434844, San Diego, California 92143‐4844 USA.</p></note></noteGroup></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Proteolytic Activity in California Halibut Larvae (Paralichthys californicus)</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Proteolytic Activity in California Halibut Larvae (Paralichthys californicus)</title></titleInfo><name type="personal"><namePart type="given">Magali</namePart><namePart type="family">Zacarias‐Soto</namePart><affiliation>Department of Aquaculture, Center for Scientific Research and Higher Education of Ensenada. 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Increasing our knowledge of the digestive capacity and nutritional requirements of the larvae of new candidate species for aquaculture will aid in the development of optimal feeding protocols and greatly improve production under hatchery conditions. In this study, we assess the proteolytic capacity of California halibut (Paralichthys californicus) larvae using biochemical and histological analyses. Newly hatched larvae were reared in a semiclosed recirculating system and fed with highly unsaturated fatty acid (HUFA)–enriched rotifers from hatching until 19 d posthatch (dph) and HUFA‐enriched Artemia nauplii thereafter. Total and specific activity of trypsin and leucine‐aminopeptidase (LAP) and acid and alkaline protease activities were assessed throughout development using spectrophotometric techniques. Trypsin‐like activity and LAP and alkaline protease activities were detected shortly after hatching and before the opening of the mouth. Acid protease activity was not detected until 36–40 dph, concomitant with the development of the gastric glands. The specific activity of trypsin and LAP showed two distinct peaks at 8 and 20 dph. The second peak coincided with the shift from rotifers to Artemia. Hence, newly hatched California halibut larvae possess alkaline proteolytic activity before first feeding. Based on the digestive capacity evaluated in this study and the timing of the development of the functional stomach, we propose that California halibut can be adequately weaned to formulated microdiets around 36 dph.</abstract><relatedItem type="host"><titleInfo><title>Journal of the World Aquaculture Society</title></titleInfo><genre type="journal">journal</genre><identifier type="ISSN">0893-8849</identifier><identifier type="eISSN">1749-7345</identifier><identifier type="DOI">10.1111/(ISSN)1749-7345</identifier><identifier type="PublisherID">JWAS</identifier><part><date>2006</date><detail type="volume"><caption>vol.</caption><number>37</number></detail><detail type="issue"><caption>no.</caption><number>2</number></detail><extent unit="pages"><start>175</start><end>185</end><total>11</total></extent></part></relatedItem><identifier type="istex">5CB84C406AF118A9F0E524CA6848FF1893ED1FEE</identifier><identifier type="DOI">10.1111/j.1749-7345.2006.00024.x</identifier><identifier type="ArticleID">JWAS24</identifier><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>Blackwell Publishing Inc</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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|texte= Proteolytic Activity in California Halibut Larvae (Paralichthys californicus)
}}
| This area was generated with Dilib version V0.6.27. |  |