A Molecular Approach to Control the International Trade in Black Caviar
Identifieur interne : 001063 ( Istex/Corpus ); précédent : 001062; suivant : 001064A Molecular Approach to Control the International Trade in Black Caviar
Auteurs : Arne Ludwig ; Lutz Debus ; Ingo JenneckensSource :
- International Review of Hydrobiology [ 1434-2944 ] ; 2002-11.
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- KwdEn :
Abstract
The aim of this study was to develop and establish a molecular method for species identification of sturgeon products, esp. black caviar, used in the international trade. Sequences of the entire cytochrome b (cytb) gene from 858 fish specimens were used for discriminating between 22 sturgeon species, and potential species‐specific restriction sites were determined. No single restriction endonuclease can be used for the differentiation of all species. Depending on the species, from one to four different enzymes are necessary for species identification. Overall, using seven different restriction endonucleases, 17 acipenseriform species can be separated on the mtDNA level on the basis of characteristic species‐specific restriction patterns. Three species of the genus Scaphirhynchus (S. albus, S. platorhynchus and S. suttkusi), as well as Acipenser gueldenstaedti and A. persicus, were not differentiated. Our approach provides an opportunity to identify and control the trade in sturgeon products outside of the three main caviar producing species, A. gueldenstaedti, A. stellatus, and Huso huso. Besides the trade, the method is important for the management and conservation programs. The necessity to combine nuclear and mtDNA markers for more precise identification is also discussed. The following hybrids were observed using mitochondrial and nuclear markers: one A. gueldenstaedti/A. stellatus hybrid, one A. gueldenstaedti/Acipenser ruthenus hybrid, five hybrids between A. gueldenstaedti or A. persicus and A. nudiventris.
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DOI: 10.1002/1522-2632(200211)87:5/6<661::AID-IROH661>3.0.CO;2-S
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Sequences of the entire cytochrome b (cytb) gene from 858 fish specimens were used for discriminating between 22 sturgeon species, and potential species‐specific restriction sites were determined. No single restriction endonuclease can be used for the differentiation of all species. Depending on the species, from one to four different enzymes are necessary for species identification. Overall, using seven different restriction endonucleases, 17 acipenseriform species can be separated on the mtDNA level on the basis of characteristic species‐specific restriction patterns. Three species of the genus Scaphirhynchus (S. albus, S. platorhynchus and S. suttkusi), as well as Acipenser gueldenstaedti and A. persicus, were not differentiated. Our approach provides an opportunity to identify and control the trade in sturgeon products outside of the three main caviar producing species, A. gueldenstaedti, A. stellatus, and Huso huso. Besides the trade, the method is important for the management and conservation programs. The necessity to combine nuclear and mtDNA markers for more precise identification is also discussed. The following hybrids were observed using mitochondrial and nuclear markers: one A. gueldenstaedti/A. stellatus hybrid, one A. gueldenstaedti/Acipenser ruthenus hybrid, five hybrids between A. gueldenstaedti or A. persicus and A. nudiventris.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>Arne Ludwig</name><affiliations><json:string>Institut für Gewässerökologie und Binnenfischerei, Abteilung für Biologie und Ökologie der Fische, 12561 Berlin, Germany</json:string><json:string>Current Address: Institut für Zoo‐ und Wildtierforschung, Fachgebiet Evolutionsgenetik, PF 601103, 10252 Berlin, Germany</json:string><json:string>E-mail: Ludwig@izw‐berlin.de</json:string></affiliations></json:item><json:item><name>Lutz Debus</name><affiliations><json:string>Institut für Zoologie, Universität Rostock, Germany</json:string></affiliations></json:item><json:item><name>Ingo Jenneckens</name><affiliations><json:string>Institut für Tierzucht und Genetik, Universität Göttingen, Germany</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>species identification</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>sturgeon</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>cytochrome b</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>RFLP</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>mtDNA</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>conservation</value></json:item></subject><articleId><json:string>IROH661</json:string></articleId><language><json:string>eng</json:string></language><originalGenre><json:string>article</json:string></originalGenre><abstract>The aim of this study was to develop and establish a molecular method for species identification of sturgeon products, esp. black caviar, used in the international trade. 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Sequences of the entire cytochrome b (cytb) gene from 858 fish specimens were used for discriminating between 22 sturgeon species, and potential species‐specific restriction sites were determined. No single restriction endonuclease can be used for the differentiation of all species. Depending on the species, from one to four different enzymes are necessary for species identification. Overall, using seven different restriction endonucleases, 17 acipenseriform species can be separated on the mtDNA level on the basis of characteristic species‐specific restriction patterns. Three species of the genus Scaphirhynchus (S. albus, S. platorhynchus and S. suttkusi), as well as Acipenser gueldenstaedti and A. persicus, were not differentiated. Our approach provides an opportunity to identify and control the trade in sturgeon products outside of the three main caviar producing species, A. gueldenstaedti, A. stellatus, and Huso huso. Besides the trade, the method is important for the management and conservation programs. The necessity to combine nuclear and mtDNA markers for more precise identification is also discussed. The following hybrids were observed using mitochondrial and nuclear markers: one A. gueldenstaedti/A. stellatus hybrid, one A. gueldenstaedti/Acipenser ruthenus hybrid, five hybrids between A. gueldenstaedti or A. persicus and A. nudiventris.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>keywords</head><item><term>species identification</term></item><item><term>sturgeon</term></item><item><term>cytochrome b</term></item><item><term>RFLP</term></item><item><term>mtDNA</term></item><item><term>conservation</term></item></list></keywords></textClass><textClass><keywords scheme="Journal Subject"><list><head>article-category</head><item><term>IV. 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Sequences of the entire cytochrome <i>b</i> (cyt<i>b</i>) gene from 858 fish specimens were used for discriminating between 22 sturgeon species, and potential species‐specific restriction sites were determined. No single restriction endonuclease can be used for the differentiation of all species. Depending on the species, from one to four different enzymes are necessary for species identification. Overall, using seven different restriction endonucleases, 17 acipenseriform species can be separated on the mtDNA level on the basis of characteristic species‐specific restriction patterns. Three species of the genus <i>Scaphirhynchus</i> (<i>S. albus</i>, <i>S. platorhynchus</i> and <i>S. suttkusi</i>), as well as <i>Acipenser gueldenstaedti</i> and <i>A. persicus,</i> were not differentiated. Our approach provides an opportunity to identify and control the trade in sturgeon products outside of the three main caviar producing species, <i>A. gueldenstaedti</i>, <i>A. stellatus,</i> and <i>Huso huso</i>. Besides the trade, the method is important for the management and conservation programs. The necessity to combine nuclear and mtDNA markers for more precise identification is also discussed. The following hybrids were observed using mitochondrial and nuclear markers: one <i>A. gueldenstaedti</i>/<i>A. stellatus</i> hybrid, one <i>A. gueldenstaedti</i>/<i>Acipenser ruthenus</i> hybrid, five hybrids between <i>A. gueldenstaedti</i> or <i>A. persicus</i> and <i>A. nudiventris</i>.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>A Molecular Approach to Control the International Trade in Black Caviar</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>A Molecular Approach to Control Trade in Caviar</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>A Molecular Approach to Control the International Trade in Black Caviar</title></titleInfo><name type="personal"><namePart type="given">Arne</namePart><namePart type="family">Ludwig</namePart><affiliation>Institut für Gewässerökologie und Binnenfischerei, Abteilung für Biologie und Ökologie der Fische, 12561 Berlin, Germany</affiliation><affiliation>Current Address: Institut für Zoo‐ und Wildtierforschung, Fachgebiet Evolutionsgenetik, PF 601103, 10252 Berlin, Germany</affiliation><affiliation>E-mail: Ludwig@izw‐berlin.de</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Lutz</namePart><namePart type="family">Debus</namePart><affiliation>Institut für Zoologie, Universität Rostock, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Ingo</namePart><namePart type="family">Jenneckens</namePart><affiliation>Institut für Tierzucht und Genetik, Universität Göttingen, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>WILEY‐VCH Verlag</publisher><place><placeTerm type="text">Berlin</placeTerm></place><dateIssued encoding="w3cdtf">2002-11</dateIssued><copyrightDate encoding="w3cdtf">2002</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">1</extent><extent unit="tables">5</extent><extent unit="references">41</extent></physicalDescription><abstract lang="en">The aim of this study was to develop and establish a molecular method for species identification of sturgeon products, esp. black caviar, used in the international trade. Sequences of the entire cytochrome b (cytb) gene from 858 fish specimens were used for discriminating between 22 sturgeon species, and potential species‐specific restriction sites were determined. No single restriction endonuclease can be used for the differentiation of all species. Depending on the species, from one to four different enzymes are necessary for species identification. Overall, using seven different restriction endonucleases, 17 acipenseriform species can be separated on the mtDNA level on the basis of characteristic species‐specific restriction patterns. Three species of the genus Scaphirhynchus (S. albus, S. platorhynchus and S. suttkusi), as well as Acipenser gueldenstaedti and A. persicus, were not differentiated. Our approach provides an opportunity to identify and control the trade in sturgeon products outside of the three main caviar producing species, A. gueldenstaedti, A. stellatus, and Huso huso. Besides the trade, the method is important for the management and conservation programs. The necessity to combine nuclear and mtDNA markers for more precise identification is also discussed. The following hybrids were observed using mitochondrial and nuclear markers: one A. gueldenstaedti/A. stellatus hybrid, one A. gueldenstaedti/Acipenser ruthenus hybrid, five hybrids between A. gueldenstaedti or A. persicus and A. nudiventris.</abstract><subject lang="en"><genre>keywords</genre><topic>species identification</topic><topic>sturgeon</topic><topic>cytochrome b</topic><topic>RFLP</topic><topic>mtDNA</topic><topic>conservation</topic></subject><relatedItem type="host"><titleInfo><title>International Review of Hydrobiology</title><subTitle>A Journal Covering all Aspects of Limnology and Marine Biology</subTitle></titleInfo><titleInfo type="abbreviated"><title>International Review of Hydrobiology</title></titleInfo><genre type="journal">journal</genre><subject><genre>article-category</genre><topic>IV. Chemical and Biochemical Composition of Sturgeon Products: Original Paper</topic></subject><identifier type="ISSN">1434-2944</identifier><identifier type="eISSN">1522-2632</identifier><identifier type="DOI">10.1002/(ISSN)1522-2632</identifier><identifier type="PublisherID">IROH</identifier><part><date>2002</date><detail type="volume"><caption>vol.</caption><number>87</number></detail><detail type="issue"><caption>no.</caption><number>5‐6</number></detail><extent unit="pages"><start>661</start><end>674</end><total>14</total></extent></part></relatedItem><identifier type="istex">AA542C0EAFC9DB814F86E4A1E0FA8363AF6F24A5</identifier><identifier type="DOI">10.1002/1522-2632(200211)87:5/6<661::AID-IROH661>3.0.CO;2-S</identifier><identifier type="ArticleID">IROH661</identifier><accessCondition type="use and reproduction" contentType="copyright">© 2002 WILEY‐VCH Verlag GmbH & Co. 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