Regulation of hepatic lipogenesis by dietary protein/energy in juvenile European seabass ( Dicentrarchus labrax )
Identifieur interne : 001054 ( Istex/Corpus ); précédent : 001053; suivant : 001055Regulation of hepatic lipogenesis by dietary protein/energy in juvenile European seabass ( Dicentrarchus labrax )
Auteurs : J. Dias ; M. J Alvarez ; A. Diez ; J. Arzel ; G. Corraze ; J. M Bautista ; S. J KaushikSource :
- Aquaculture [ 0044-8486 ] ; 1998.
Abstract
A growth trial was conducted with groups of European seabass having an initial weight of 6 g to study the lipogenic action of dietary protein and non-protein energy supplies. Six experimental diets were formulated to contain one of two crude protein levels (43 and 52%) with digestible protein (DP) to digestible energy (DE) ratios ranging from 19 to 26 mg/kJ. At the end of the growth trial (12 weeks), the activities of liver glucose-6-phosphate dehydrogenase (G6PD, EC 1.1.1.49), malic enzyme (ME, EC 1.1.1.40), ATP citrate lyase (ACL, EC 4.1.3.8), acetyl-CoA carboxylase (ACoAC, EC 6.4.1.2) and fatty acid synthetase (FAS, EC 2.3.1.38) were measured. Digestibility of main dietary components was also determined over a three-week period. At each protein level, an increase in dietary DE led to improved growth performance, protein efficiency, daily N deposition and to a reduction of N loss. Best results were achieved at 40% DP and a DP/DE ratio of 19–20 mg/kJ. G6PD, ME and ACoAC were found to be the key regulatory enzymes in the lipogenic pathway, with G6PD being the main NADPH-generating enzyme. Activities of G6PD, ME, ACL and FAS were reduced with increasing fat intake. Activities of G6PD, ME and ACL were increased with increasing starch intake. ACoAC activity was negatively correlated with starch intake and positively with fat intake.
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DOI: 10.1016/S0044-8486(97)00268-8
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Diez</name><affiliation><mods:affiliation>Departemento de Bioquimica y Biologia Molécular IV, Universidad Complutense de Madrid, Facultad de Veterinaria, 28040 Madrid, Spain</mods:affiliation></affiliation></author><author><name sortKey="Arzel, J" sort="Arzel, J" uniqKey="Arzel J" first="J" last="Arzel">J. Arzel</name><affiliation><mods:affiliation>Fish Nutrition Laboratory, Unité Mixte INRA-IFREMER, Centre de Brest IFREMER, 29280 Plouzané, France</mods:affiliation></affiliation></author><author><name sortKey="Corraze, G" sort="Corraze, G" uniqKey="Corraze G" first="G" last="Corraze">G. Corraze</name><affiliation><mods:affiliation>Fish Nutrition Laboratory, Unité Mixte INRA-IFREMER, 64310 Saint Pée-sur-Nivelle, France</mods:affiliation></affiliation></author><author><name sortKey="Bautista, J M" sort="Bautista, J M" uniqKey="Bautista J" first="J. M" last="Bautista">J. M Bautista</name><affiliation><mods:affiliation>Departemento de Bioquimica y Biologia Molécular IV, Universidad Complutense de Madrid, Facultad de Veterinaria, 28040 Madrid, Spain</mods:affiliation></affiliation></author><author><name sortKey="Kaushik, S J" sort="Kaushik, S J" uniqKey="Kaushik S" first="S. J" last="Kaushik">S. 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Dias</name><affiliation><mods:affiliation>Fish Nutrition Laboratory, Unité Mixte INRA-IFREMER, 64310 Saint Pée-sur-Nivelle, France</mods:affiliation></affiliation></author><author><name sortKey="Alvarez, M J" sort="Alvarez, M J" uniqKey="Alvarez M" first="M. J" last="Alvarez">M. J Alvarez</name><affiliation><mods:affiliation>Departemento de Bioquimica y Biologia Molécular IV, Universidad Complutense de Madrid, Facultad de Veterinaria, 28040 Madrid, Spain</mods:affiliation></affiliation></author><author><name sortKey="Diez, A" sort="Diez, A" uniqKey="Diez A" first="A" last="Diez">A. Diez</name><affiliation><mods:affiliation>Departemento de Bioquimica y Biologia Molécular IV, Universidad Complutense de Madrid, Facultad de Veterinaria, 28040 Madrid, Spain</mods:affiliation></affiliation></author><author><name sortKey="Arzel, J" sort="Arzel, J" uniqKey="Arzel J" first="J" last="Arzel">J. Arzel</name><affiliation><mods:affiliation>Fish Nutrition Laboratory, Unité Mixte INRA-IFREMER, Centre de Brest IFREMER, 29280 Plouzané, France</mods:affiliation></affiliation></author><author><name sortKey="Corraze, G" sort="Corraze, G" uniqKey="Corraze G" first="G" last="Corraze">G. Corraze</name><affiliation><mods:affiliation>Fish Nutrition Laboratory, Unité Mixte INRA-IFREMER, 64310 Saint Pée-sur-Nivelle, France</mods:affiliation></affiliation></author><author><name sortKey="Bautista, J M" sort="Bautista, J M" uniqKey="Bautista J" first="J. M" last="Bautista">J. M Bautista</name><affiliation><mods:affiliation>Departemento de Bioquimica y Biologia Molécular IV, Universidad Complutense de Madrid, Facultad de Veterinaria, 28040 Madrid, Spain</mods:affiliation></affiliation></author><author><name sortKey="Kaushik, S J" sort="Kaushik, S J" uniqKey="Kaushik S" first="S. J" last="Kaushik">S. J Kaushik</name><affiliation><mods:affiliation>Fish Nutrition Laboratory, Unité Mixte INRA-IFREMER, 64310 Saint Pée-sur-Nivelle, France</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Aquaculture</title><title level="j" type="abbrev">AQUA</title><idno type="ISSN">0044-8486</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1998">1998</date><biblScope unit="volume">161</biblScope><biblScope unit="issue">1–4</biblScope><biblScope unit="page" from="169">169</biblScope><biblScope unit="page" to="186">186</biblScope></imprint><idno type="ISSN">0044-8486</idno></series><idno type="istex">5A5147C0BCF9F6937EC4149DB0D680B517705B7C</idno><idno type="DOI">10.1016/S0044-8486(97)00268-8</idno><idno type="PII">S0044-8486(97)00268-8</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0044-8486</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A growth trial was conducted with groups of European seabass having an initial weight of 6 g to study the lipogenic action of dietary protein and non-protein energy supplies. Six experimental diets were formulated to contain one of two crude protein levels (43 and 52%) with digestible protein (DP) to digestible energy (DE) ratios ranging from 19 to 26 mg/kJ. At the end of the growth trial (12 weeks), the activities of liver glucose-6-phosphate dehydrogenase (G6PD, EC 1.1.1.49), malic enzyme (ME, EC 1.1.1.40), ATP citrate lyase (ACL, EC 4.1.3.8), acetyl-CoA carboxylase (ACoAC, EC 6.4.1.2) and fatty acid synthetase (FAS, EC 2.3.1.38) were measured. Digestibility of main dietary components was also determined over a three-week period. At each protein level, an increase in dietary DE led to improved growth performance, protein efficiency, daily N deposition and to a reduction of N loss. Best results were achieved at 40% DP and a DP/DE ratio of 19–20 mg/kJ. G6PD, ME and ACoAC were found to be the key regulatory enzymes in the lipogenic pathway, with G6PD being the main NADPH-generating enzyme. Activities of G6PD, ME, ACL and FAS were reduced with increasing fat intake. Activities of G6PD, ME and ACL were increased with increasing starch intake. ACoAC activity was negatively correlated with starch intake and positively with fat intake.</div></front></TEI><istex><corpusName>elsevier</corpusName><author><json:item><name>J Dias</name><affiliations><json:string>Fish Nutrition Laboratory, Unité Mixte INRA-IFREMER, 64310 Saint Pée-sur-Nivelle, France</json:string></affiliations></json:item><json:item><name>M.J Alvarez</name><affiliations><json:string>Departemento de Bioquimica y Biologia Molécular IV, Universidad Complutense de Madrid, Facultad de Veterinaria, 28040 Madrid, Spain</json:string></affiliations></json:item><json:item><name>A Diez</name><affiliations><json:string>Departemento de Bioquimica y Biologia Molécular IV, Universidad Complutense de Madrid, Facultad de Veterinaria, 28040 Madrid, Spain</json:string></affiliations></json:item><json:item><name>J Arzel</name><affiliations><json:string>Fish Nutrition Laboratory, Unité Mixte INRA-IFREMER, Centre de Brest IFREMER, 29280 Plouzané, France</json:string></affiliations></json:item><json:item><name>G Corraze</name><affiliations><json:string>Fish Nutrition Laboratory, Unité Mixte INRA-IFREMER, 64310 Saint Pée-sur-Nivelle, France</json:string></affiliations></json:item><json:item><name>J.M Bautista</name><affiliations><json:string>Departemento de Bioquimica y Biologia Molécular IV, Universidad Complutense de Madrid, Facultad de Veterinaria, 28040 Madrid, Spain</json:string></affiliations></json:item><json:item><name>S.J Kaushik</name><affiliations><json:string>Fish Nutrition Laboratory, Unité Mixte INRA-IFREMER, 64310 Saint Pée-sur-Nivelle, France</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>European seabass</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Dietary energy</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Lipid deposition</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Lipogenesis</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Liver</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Enzymes</value></json:item></subject><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>A growth trial was conducted with groups of European seabass having an initial weight of 6 g to study the lipogenic action of dietary protein and non-protein energy supplies. Six experimental diets were formulated to contain one of two crude protein levels (43 and 52%) with digestible protein (DP) to digestible energy (DE) ratios ranging from 19 to 26 mg/kJ. At the end of the growth trial (12 weeks), the activities of liver glucose-6-phosphate dehydrogenase (G6PD, EC 1.1.1.49), malic enzyme (ME, EC 1.1.1.40), ATP citrate lyase (ACL, EC 4.1.3.8), acetyl-CoA carboxylase (ACoAC, EC 6.4.1.2) and fatty acid synthetase (FAS, EC 2.3.1.38) were measured. Digestibility of main dietary components was also determined over a three-week period. At each protein level, an increase in dietary DE led to improved growth performance, protein efficiency, daily N deposition and to a reduction of N loss. Best results were achieved at 40% DP and a DP/DE ratio of 19–20 mg/kJ. G6PD, ME and ACoAC were found to be the key regulatory enzymes in the lipogenic pathway, with G6PD being the main NADPH-generating enzyme. Activities of G6PD, ME, ACL and FAS were reduced with increasing fat intake. Activities of G6PD, ME and ACL were increased with increasing starch intake. ACoAC activity was negatively correlated with starch intake and positively with fat intake.</abstract><qualityIndicators><score>7.592</score><pdfVersion>1.2</pdfVersion><pdfPageSize>435 x 643 pts</pdfPageSize><refBibsNative>true</refBibsNative><keywordCount>6</keywordCount><abstractCharCount>1351</abstractCharCount><pdfWordCount>6803</pdfWordCount><pdfCharCount>43416</pdfCharCount><pdfPageCount>18</pdfPageCount><abstractWordCount>216</abstractWordCount></qualityIndicators><title>Regulation of hepatic lipogenesis by dietary protein/energy in juvenile European seabass ( Dicentrarchus labrax )</title><pii><json:string>S0044-8486(97)00268-8</json:string></pii><genre><json:string>research-article</json:string></genre><host><volume>161</volume><pii><json:string>S0044-8486(00)X0062-2</json:string></pii><pages><last>186</last><first>169</first></pages><issn><json:string>0044-8486</json:string></issn><issue>1–4</issue><genre><json:string>journal</json:string></genre><language><json:string>unknown</json:string></language><title>Aquaculture</title><publicationDate>1998</publicationDate></host><publicationDate>1998</publicationDate><copyrightDate>1998</copyrightDate><doi><json:string>10.1016/S0044-8486(97)00268-8</json:string></doi><id>5A5147C0BCF9F6937EC4149DB0D680B517705B7C</id><score>0.02558224</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/document/5A5147C0BCF9F6937EC4149DB0D680B517705B7C/fulltext/pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/document/5A5147C0BCF9F6937EC4149DB0D680B517705B7C/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/5A5147C0BCF9F6937EC4149DB0D680B517705B7C/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a">Regulation of hepatic lipogenesis by dietary protein/energy in juvenile European seabass ( Dicentrarchus labrax )</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>ELSEVIER</publisher><availability><p>©1998 Elsevier Science B.V.</p></availability><date>1998</date></publicationStmt><notesStmt><note type="content">Fig. 1: Activities of hepatic lipogenic enzymes in juvenile seabass as affected by different dietary protein to energy ratios. The numbers in the x-axis correspond to dietary treatments as described in Table 1.</note><note type="content">Table 1: Ingredient and proximate composition of the experimental diets</note><note type="content">Table 2: Apparent digestibility coefficients (ADC) of the dietary components.</note><note type="content">Table 3: Effects of dietary protein to energy ratios on growth performance, feed utilization efficiency, nutrient and energy retention and N balance in juvenile seabass grown over 12 weeks at 18°C (IBW1=5.9 g)</note><note type="content">Table 4: Effects of dietary protein to energy ratios on HSI, VSI and tissue lipid content in juvenile seabass</note><note type="content">Table 5: Muscle fatty acid composition of seabass fed the different experimental diets</note><note type="content">Table 6: Effects of dietary protein to energy ratios on hepatic lipogenic enzyme activities</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">Regulation of hepatic lipogenesis by dietary protein/energy in juvenile European seabass ( Dicentrarchus labrax )</title><author xml:id="author-1"><persName><forename type="first">J</forename><surname>Dias</surname></persName><note type="biography">J. Dias and M.J. Alvarez made equal contributions to the work presented here.</note><affiliation>J. Dias and M.J. Alvarez made equal contributions to the work presented here.</affiliation><affiliation>Fish Nutrition Laboratory, Unité Mixte INRA-IFREMER, 64310 Saint Pée-sur-Nivelle, France</affiliation></author><author xml:id="author-2"><persName><forename type="first">M.J</forename><surname>Alvarez</surname></persName><note type="biography">J. Dias and M.J. Alvarez made equal contributions to the work presented here.</note><affiliation>J. Dias and M.J. Alvarez made equal contributions to the work presented here.</affiliation><affiliation>Departemento de Bioquimica y Biologia Molécular IV, Universidad Complutense de Madrid, Facultad de Veterinaria, 28040 Madrid, Spain</affiliation></author><author xml:id="author-3"><persName><forename type="first">A</forename><surname>Diez</surname></persName><affiliation>Departemento de Bioquimica y Biologia Molécular IV, Universidad Complutense de Madrid, Facultad de Veterinaria, 28040 Madrid, Spain</affiliation></author><author xml:id="author-4"><persName><forename type="first">J</forename><surname>Arzel</surname></persName><affiliation>Fish Nutrition Laboratory, Unité Mixte INRA-IFREMER, Centre de Brest IFREMER, 29280 Plouzané, France</affiliation></author><author xml:id="author-5"><persName><forename type="first">G</forename><surname>Corraze</surname></persName><affiliation>Fish Nutrition Laboratory, Unité Mixte INRA-IFREMER, 64310 Saint Pée-sur-Nivelle, France</affiliation></author><author xml:id="author-6"><persName><forename type="first">J.M</forename><surname>Bautista</surname></persName><affiliation>Departemento de Bioquimica y Biologia Molécular IV, Universidad Complutense de Madrid, Facultad de Veterinaria, 28040 Madrid, Spain</affiliation></author><author xml:id="author-7"><persName><forename type="first">S.J</forename><surname>Kaushik</surname></persName><affiliation>Fish Nutrition Laboratory, Unité Mixte INRA-IFREMER, 64310 Saint Pée-sur-Nivelle, France</affiliation></author></analytic><monogr><title level="j">Aquaculture</title><title level="j" type="abbrev">AQUA</title><idno type="pISSN">0044-8486</idno><idno type="PII">S0044-8486(00)X0062-2</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1998"></date><biblScope unit="volume">161</biblScope><biblScope unit="issue">1–4</biblScope><biblScope unit="page" from="169">169</biblScope><biblScope unit="page" to="186">186</biblScope></imprint></monogr><idno type="istex">5A5147C0BCF9F6937EC4149DB0D680B517705B7C</idno><idno type="DOI">10.1016/S0044-8486(97)00268-8</idno><idno type="PII">S0044-8486(97)00268-8</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1998</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>A growth trial was conducted with groups of European seabass having an initial weight of 6 g to study the lipogenic action of dietary protein and non-protein energy supplies. Six experimental diets were formulated to contain one of two crude protein levels (43 and 52%) with digestible protein (DP) to digestible energy (DE) ratios ranging from 19 to 26 mg/kJ. At the end of the growth trial (12 weeks), the activities of liver glucose-6-phosphate dehydrogenase (G6PD, EC 1.1.1.49), malic enzyme (ME, EC 1.1.1.40), ATP citrate lyase (ACL, EC 4.1.3.8), acetyl-CoA carboxylase (ACoAC, EC 6.4.1.2) and fatty acid synthetase (FAS, EC 2.3.1.38) were measured. Digestibility of main dietary components was also determined over a three-week period. At each protein level, an increase in dietary DE led to improved growth performance, protein efficiency, daily N deposition and to a reduction of N loss. Best results were achieved at 40% DP and a DP/DE ratio of 19–20 mg/kJ. G6PD, ME and ACoAC were found to be the key regulatory enzymes in the lipogenic pathway, with G6PD being the main NADPH-generating enzyme. Activities of G6PD, ME, ACL and FAS were reduced with increasing fat intake. Activities of G6PD, ME and ACL were increased with increasing starch intake. ACoAC activity was negatively correlated with starch intake and positively with fat intake.</p></abstract><textClass><keywords scheme="keyword"><list><head>Keywords</head><item><term>European seabass</term></item><item><term>Dietary energy</term></item><item><term>Lipid deposition</term></item><item><term>Lipogenesis</term></item><item><term>Liver</term></item><item><term>Enzymes</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1998">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/5A5147C0BCF9F6937EC4149DB0D680B517705B7C/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: ce:floats; body; tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"><istex:entity SYSTEM="gr1" NDATA="IMAGE" name="gr1"></istex:entity></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>AQUA</jid><aid>60388</aid><ce:pii>S0044-8486(97)00268-8</ce:pii><ce:doi>10.1016/S0044-8486(97)00268-8</ce:doi><ce:copyright year="1998" type="full-transfer">Elsevier Science B.V.</ce:copyright></item-info><head><ce:title>Regulation of hepatic lipogenesis by dietary protein/energy in juvenile European seabass (<ce:italic>Dicentrarchus labrax</ce:italic>)</ce:title><ce:author-group><ce:author><ce:given-name>J</ce:given-name><ce:surname>Dias</ce:surname><ce:cross-ref refid="AFF1">a</ce:cross-ref><ce:cross-ref refid="FN1"><ce:sup>1</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>M.J</ce:given-name><ce:surname>Alvarez</ce:surname><ce:cross-ref refid="AFF2">b</ce:cross-ref><ce:cross-ref refid="FN1"><ce:sup>1</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>A</ce:given-name><ce:surname>Diez</ce:surname><ce:cross-ref refid="AFF2">b</ce:cross-ref></ce:author><ce:author><ce:given-name>J</ce:given-name><ce:surname>Arzel</ce:surname><ce:cross-ref refid="AFF3">c</ce:cross-ref></ce:author><ce:author><ce:given-name>G</ce:given-name><ce:surname>Corraze</ce:surname><ce:cross-ref refid="AFF1">a</ce:cross-ref></ce:author><ce:author><ce:given-name>J.M</ce:given-name><ce:surname>Bautista</ce:surname><ce:cross-ref refid="AFF2">b</ce:cross-ref></ce:author><ce:author><ce:given-name>S.J</ce:given-name><ce:surname>Kaushik</ce:surname><ce:cross-ref refid="AFF1">a</ce:cross-ref><ce:cross-ref refid="CORR1">*</ce:cross-ref></ce:author><ce:affiliation id="AFF1"><ce:label>a</ce:label><ce:textfn>Fish Nutrition Laboratory, Unité Mixte INRA-IFREMER, 64310 Saint Pée-sur-Nivelle, France</ce:textfn></ce:affiliation><ce:affiliation id="AFF2"><ce:label>b</ce:label><ce:textfn>Departemento de Bioquimica y Biologia Molécular IV, Universidad Complutense de Madrid, Facultad de Veterinaria, 28040 Madrid, Spain</ce:textfn></ce:affiliation><ce:affiliation id="AFF3"><ce:label>c</ce:label><ce:textfn>Fish Nutrition Laboratory, Unité Mixte INRA-IFREMER, Centre de Brest IFREMER, 29280 Plouzané, France</ce:textfn></ce:affiliation><ce:correspondence id="CORR1"><ce:label>*</ce:label><ce:text>Corresponding author. Tel.: +33-0-5-59-515951; Fax.: +33-0-5-59-545152; E-mail: kaushik@st-pee.inra.fr.</ce:text></ce:correspondence><ce:footnote id="FN1"><ce:label>1</ce:label><ce:note-para>J. Dias and M.J. Alvarez made equal contributions to the work presented here.</ce:note-para></ce:footnote></ce:author-group><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>A growth trial was conducted with groups of European seabass having an initial weight of 6 g to study the lipogenic action of dietary protein and non-protein energy supplies. Six experimental diets were formulated to contain one of two crude protein levels (43 and 52%) with digestible protein (DP) to digestible energy (DE) ratios ranging from 19 to 26 mg/kJ. At the end of the growth trial (12 weeks), the activities of liver glucose-6-phosphate dehydrogenase (G6PD, EC 1.1.1.49), malic enzyme (ME, EC 1.1.1.40), ATP citrate lyase (ACL, EC 4.1.3.8), acetyl-CoA carboxylase (ACoAC, EC 6.4.1.2) and fatty acid synthetase (FAS, EC 2.3.1.38) were measured. Digestibility of main dietary components was also determined over a three-week period. At each protein level, an increase in dietary DE led to improved growth performance, protein efficiency, daily N deposition and to a reduction of N loss. Best results were achieved at 40% DP and a DP/DE ratio of 19–20 mg/kJ. G6PD, ME and ACoAC were found to be the key regulatory enzymes in the lipogenic pathway, with G6PD being the main NADPH-generating enzyme. Activities of G6PD, ME, ACL and FAS were reduced with increasing fat intake. Activities of G6PD, ME and ACL were increased with increasing starch intake. ACoAC activity was negatively correlated with starch intake and positively with fat intake.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords class="keyword"><ce:section-title>Keywords</ce:section-title><ce:keyword><ce:text>European seabass</ce:text></ce:keyword><ce:keyword><ce:text>Dietary energy</ce:text></ce:keyword><ce:keyword><ce:text>Lipid deposition</ce:text></ce:keyword><ce:keyword><ce:text>Lipogenesis</ce:text></ce:keyword><ce:keyword><ce:text>Liver</ce:text></ce:keyword><ce:keyword><ce:text>Enzymes</ce:text></ce:keyword></ce:keywords></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Regulation of hepatic lipogenesis by dietary protein/energy in juvenile European seabass ( Dicentrarchus labrax )</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Regulation of hepatic lipogenesis by dietary protein/energy in juvenile European seabass (</title></titleInfo><name type="personal"><namePart type="given">J</namePart><namePart type="family">Dias</namePart><affiliation>Fish Nutrition Laboratory, Unité Mixte INRA-IFREMER, 64310 Saint Pée-sur-Nivelle, France</affiliation><description>J. Dias and M.J. Alvarez made equal contributions to the work presented here.</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.J</namePart><namePart type="family">Alvarez</namePart><affiliation>Departemento de Bioquimica y Biologia Molécular IV, Universidad Complutense de Madrid, Facultad de Veterinaria, 28040 Madrid, Spain</affiliation><description>J. Dias and M.J. Alvarez made equal contributions to the work presented here.</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">A</namePart><namePart type="family">Diez</namePart><affiliation>Departemento de Bioquimica y Biologia Molécular IV, Universidad Complutense de Madrid, Facultad de Veterinaria, 28040 Madrid, Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J</namePart><namePart type="family">Arzel</namePart><affiliation>Fish Nutrition Laboratory, Unité Mixte INRA-IFREMER, Centre de Brest IFREMER, 29280 Plouzané, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">G</namePart><namePart type="family">Corraze</namePart><affiliation>Fish Nutrition Laboratory, Unité Mixte INRA-IFREMER, 64310 Saint Pée-sur-Nivelle, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J.M</namePart><namePart type="family">Bautista</namePart><affiliation>Departemento de Bioquimica y Biologia Molécular IV, Universidad Complutense de Madrid, Facultad de Veterinaria, 28040 Madrid, Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S.J</namePart><namePart type="family">Kaushik</namePart><affiliation>Fish Nutrition Laboratory, Unité Mixte INRA-IFREMER, 64310 Saint Pée-sur-Nivelle, France</affiliation><description>Corresponding author. Tel.: +33-0-5-59-515951; Fax.: +33-0-5-59-545152; E-mail: kaushik@st-pee.inra.fr.</description><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article"></genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1998</dateIssued><copyrightDate encoding="w3cdtf">1998</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">A growth trial was conducted with groups of European seabass having an initial weight of 6 g to study the lipogenic action of dietary protein and non-protein energy supplies. Six experimental diets were formulated to contain one of two crude protein levels (43 and 52%) with digestible protein (DP) to digestible energy (DE) ratios ranging from 19 to 26 mg/kJ. At the end of the growth trial (12 weeks), the activities of liver glucose-6-phosphate dehydrogenase (G6PD, EC 1.1.1.49), malic enzyme (ME, EC 1.1.1.40), ATP citrate lyase (ACL, EC 4.1.3.8), acetyl-CoA carboxylase (ACoAC, EC 6.4.1.2) and fatty acid synthetase (FAS, EC 2.3.1.38) were measured. Digestibility of main dietary components was also determined over a three-week period. At each protein level, an increase in dietary DE led to improved growth performance, protein efficiency, daily N deposition and to a reduction of N loss. Best results were achieved at 40% DP and a DP/DE ratio of 19–20 mg/kJ. G6PD, ME and ACoAC were found to be the key regulatory enzymes in the lipogenic pathway, with G6PD being the main NADPH-generating enzyme. Activities of G6PD, ME, ACL and FAS were reduced with increasing fat intake. Activities of G6PD, ME and ACL were increased with increasing starch intake. ACoAC activity was negatively correlated with starch intake and positively with fat intake.</abstract><note type="content">Fig. 1: Activities of hepatic lipogenic enzymes in juvenile seabass as affected by different dietary protein to energy ratios. The numbers in the x-axis correspond to dietary treatments as described in Table 1.</note><note type="content">Table 1: Ingredient and proximate composition of the experimental diets</note><note type="content">Table 2: Apparent digestibility coefficients (ADC) of the dietary components.</note><note type="content">Table 3: Effects of dietary protein to energy ratios on growth performance, feed utilization efficiency, nutrient and energy retention and N balance in juvenile seabass grown over 12 weeks at 18°C (IBW1=5.9 g)</note><note type="content">Table 4: Effects of dietary protein to energy ratios on HSI, VSI and tissue lipid content in juvenile seabass</note><note type="content">Table 5: Muscle fatty acid composition of seabass fed the different experimental diets</note><note type="content">Table 6: Effects of dietary protein to energy ratios on hepatic lipogenic enzyme activities</note><subject><genre>Keywords</genre><topic>European seabass</topic><topic>Dietary energy</topic><topic>Lipid deposition</topic><topic>Lipogenesis</topic><topic>Liver</topic><topic>Enzymes</topic></subject><relatedItem type="host"><titleInfo><title>Aquaculture</title></titleInfo><titleInfo type="abbreviated"><title>AQUA</title></titleInfo><genre type="journal">journal</genre><originInfo><dateIssued encoding="w3cdtf">19980201</dateIssued></originInfo><identifier type="ISSN">0044-8486</identifier><identifier type="PII">S0044-8486(00)X0062-2</identifier><part><date>19980201</date><detail type="volume"><number>161</number><caption>vol.</caption></detail><detail type="issue"><number>1–4</number><caption>no.</caption></detail><extent unit="issue pages"><start>1</start><end>516</end></extent><extent unit="pages"><start>169</start><end>186</end></extent></part></relatedItem><identifier type="istex">5A5147C0BCF9F6937EC4149DB0D680B517705B7C</identifier><identifier type="DOI">10.1016/S0044-8486(97)00268-8</identifier><identifier type="PII">S0044-8486(97)00268-8</identifier><accessCondition type="use and reproduction" contentType="copyright">©1998 Elsevier Science B.V.</accessCondition><recordInfo><recordContentSource>ELSEVIER</recordContentSource><recordOrigin>Elsevier Science B.V., ©1998</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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