Isolation and characterization of Edwardsiella ictaluri strains as pathogens from diseased yellow catfish Pelteobagrus fulvidraco (Richardson) cultured in China
Identifieur interne : 001034 ( Istex/Corpus ); précédent : 001033; suivant : 001035Isolation and characterization of Edwardsiella ictaluri strains as pathogens from diseased yellow catfish Pelteobagrus fulvidraco (Richardson) cultured in China
Auteurs : Jin Yu Liu ; Ai Hua Li ; Dong Ren Zhou ; Zhou Rui Wen ; Xue Ping YeSource :
- Aquaculture Research [ 1355-557X ] ; 2010-11.
English descriptors
Abstract
Yellow catfish Pelteobagrus fulvidraco (Richardson) is a commercially important fish generally distributed in Southeast Asian countries. The well‐known aetiological agent of enteric septicaemia of catfish, Edwardsiella ictaluri, was isolated from diseased yellow catfish P. fulvidraco (Richardson) reared at two commercial fisheries in China. The economic losses due to the high mortalities (about 50%) caused by this bacterium have been increasing annually. The affected fish presented two different, typical symptoms: pale gills, slight exophthalmia and a ‘hole in the head’, and haemorrhage on the opercula, in the skin under the jaw, creating a ‘hole under the jaw’. These diseases were found frequently in cultured yellow catfish throughout China. The isolates from both outbreaks were all Gram negative, facultatively anaerobic and short rod. Morphological and biochemical tests and phylogenetic analysis based on the 16S rDNA sequences all strongly indicated that these yellow catfish isolates were highly identical to the known E. ictaluri. In addition, the isolates possessed the typical plasmid profile of E. ictaluri. Experimental infection assays were conducted and pathogenicity (by an intraperitoneal injection) was demonstrated in yellow catfish and channel catfish Ictalurus punctatus. The results showed that yellow catfish isolates were quite conservative phenotypically and genetically, and were able to cause two different, typical symptoms in this fish under unknown conditions and mechanism.
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DOI: 10.1111/j.1365-2109.2010.02571.x
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The well‐known aetiological agent of enteric septicaemia of catfish, Edwardsiella ictaluri, was isolated from diseased yellow catfish P. fulvidraco (Richardson) reared at two commercial fisheries in China. The economic losses due to the high mortalities (about 50%) caused by this bacterium have been increasing annually. The affected fish presented two different, typical symptoms: pale gills, slight exophthalmia and a ‘hole in the head’, and haemorrhage on the opercula, in the skin under the jaw, creating a ‘hole under the jaw’. These diseases were found frequently in cultured yellow catfish throughout China. The isolates from both outbreaks were all Gram negative, facultatively anaerobic and short rod. Morphological and biochemical tests and phylogenetic analysis based on the 16S rDNA sequences all strongly indicated that these yellow catfish isolates were highly identical to the known E. ictaluri. In addition, the isolates possessed the typical plasmid profile of E. ictaluri. Experimental infection assays were conducted and pathogenicity (by an intraperitoneal injection) was demonstrated in yellow catfish and channel catfish Ictalurus punctatus. 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Experimental infection assays were conducted and pathogenicity (by an intraperitoneal injection) was demonstrated in yellow catfish and channel catfish Ictalurus punctatus. 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Aquaculture Research © 2010 Blackwell Publishing Ltd</p></availability><date>2010</date></publicationStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Isolation and characterization of Edwardsiella ictaluri strains as pathogens from diseased yellow catfish Pelteobagrus fulvidraco (Richardson) cultured in China</title><author xml:id="author-1"><persName><forename type="first">Jin Yu</forename><surname>Liu</surname></persName><affiliation>Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China</affiliation><affiliation>Graduate University of Chinese Academy of Sciences, Beijing, China</affiliation></author><author xml:id="author-2"><persName><forename type="first">Ai Hua</forename><surname>Li</surname></persName><affiliation>Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China</affiliation></author><author xml:id="author-3"><persName><forename type="first">Dong Ren</forename><surname>Zhou</surname></persName><affiliation>Zhejiang Institute of Freshwater Fisheries, Huzhou Zhejiang, China</affiliation></author><author xml:id="author-4"><persName><forename type="first">Zhou Rui</forename><surname>Wen</surname></persName><affiliation>Hubei Fisheries Science Research Institute, Wuhan, China</affiliation></author><author xml:id="author-5"><persName><forename type="first">Xue Ping</forename><surname>Ye</surname></persName><affiliation>Zhejiang Institute of Freshwater Fisheries, Huzhou Zhejiang, China</affiliation></author></analytic><monogr><title level="j">Aquaculture Research</title><idno type="pISSN">1355-557X</idno><idno type="eISSN">1365-2109</idno><idno type="DOI">10.1111/(ISSN)1365-2109</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2010-11"></date><biblScope unit="volume">41</biblScope><biblScope unit="issue">12</biblScope><biblScope unit="page" from="1835">1835</biblScope><biblScope unit="page" to="1844">1844</biblScope></imprint></monogr><idno type="istex">C5ED5CDAA6121358424AB4F96459BCEE2202199E</idno><idno type="DOI">10.1111/j.1365-2109.2010.02571.x</idno><idno type="ArticleID">ARE2571</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2010</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Yellow catfish Pelteobagrus fulvidraco (Richardson) is a commercially important fish generally distributed in Southeast Asian countries. The well‐known aetiological agent of enteric septicaemia of catfish, Edwardsiella ictaluri, was isolated from diseased yellow catfish P. fulvidraco (Richardson) reared at two commercial fisheries in China. The economic losses due to the high mortalities (about 50%) caused by this bacterium have been increasing annually. The affected fish presented two different, typical symptoms: pale gills, slight exophthalmia and a ‘hole in the head’, and haemorrhage on the opercula, in the skin under the jaw, creating a ‘hole under the jaw’. These diseases were found frequently in cultured yellow catfish throughout China. The isolates from both outbreaks were all Gram negative, facultatively anaerobic and short rod. Morphological and biochemical tests and phylogenetic analysis based on the 16S rDNA sequences all strongly indicated that these yellow catfish isolates were highly identical to the known E. ictaluri. In addition, the isolates possessed the typical plasmid profile of E. ictaluri. Experimental infection assays were conducted and pathogenicity (by an intraperitoneal injection) was demonstrated in yellow catfish and channel catfish Ictalurus punctatus. The results showed that yellow catfish isolates were quite conservative phenotypically and genetically, and were able to cause two different, typical symptoms in this fish under unknown conditions and mechanism.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>keywords</head><item><term>isolation</term></item><item><term>characterization</term></item><item><term>Edwardsiella ictaluri</term></item><item><term>yellow catfish</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2010-11">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/C5ED5CDAA6121358424AB4F96459BCEE2202199E/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>Blackwell Publishing Ltd</publisherName><publisherLoc>Oxford, UK</publisherLoc></publisherInfo><doi origin="wiley" registered="yes">10.1111/(ISSN)1365-2109</doi><issn type="print">1355-557X</issn><issn type="electronic">1365-2109</issn><idGroup><id type="product" value="ARE"></id><id type="publisherDivision" value="ST"></id></idGroup><titleGroup><title type="main" sort="AQUACULTURE RESEARCH">Aquaculture Research</title></titleGroup></publicationMeta><publicationMeta level="part" position="11112"><doi origin="wiley">10.1111/are.2010.41.issue-12</doi><numberingGroup><numbering type="journalVolume" number="41">41</numbering><numbering type="journalIssue" number="12">12</numbering></numberingGroup><coverDate startDate="2010-11">November 2010</coverDate></publicationMeta><publicationMeta level="unit" type="article" position="12" status="forIssue"><doi origin="wiley">10.1111/j.1365-2109.2010.02571.x</doi><idGroup><id type="unit" value="ARE2571"></id><id type="supplier" value="2571"></id></idGroup><countGroup><count type="pageTotal" number="10"></count></countGroup><titleGroup><title type="tocHeading1">Original Articles</title></titleGroup><copyright>© 2010 The Authors. Aquaculture Research © 2010 Blackwell Publishing Ltd</copyright><eventGroup><event type="firstOnline" date="2010-11-02"></event><event type="publishedOnlineAcceptedOrEarlyUnpaginated" date="2010-07-26"></event><event type="xmlConverted" agent="Converter:BPG_TO_WML3G version:2.3.25.1 mode:FullText" date="2010-11-02"></event><event type="publishedOnlineFinalForm" date="2010-11-02"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:3.8.8" date="2014-01-06"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-10-15"></event></eventGroup><numberingGroup><numbering type="pageFirst" number="1835">1835</numbering><numbering type="pageLast" number="1844">1844</numbering></numberingGroup><correspondenceTo><b>Correspondence:</b> A H Li, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China 430072. E‐mail: <email normalForm="liaihua@ihb.ac.cn">liaihua@ihb.ac.cn</email></correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:ARE.ARE2571.pdf"></link></linkGroup></publicationMeta><contentMeta><countGroup><count type="figureTotal" number="3"></count><count type="tableTotal" number="3"></count><count type="formulaTotal" number="0"></count><count type="referenceTotal" number="50"></count><count type="wordTotal" number="7485"></count><count type="linksCrossRef" number="65"></count></countGroup><titleGroup><title type="main">Isolation and characterization of <i>Edwardsiella ictaluri</i> strains as pathogens from diseased yellow catfish <i>Pelteobagrus fulvidraco</i> (Richardson) cultured in China</title><title type="shortAuthors">J Y Liu <i>et al.</i></title><title type="short"><i>E. ictaluri</i> isolates from diseased yellow catfish</title></titleGroup><creators><creator creatorRole="author" xml:id="cr1" affiliationRef="#a1 #a2"><personName><givenNames>Jin Yu</givenNames><familyName>Liu</familyName></personName></creator><creator creatorRole="author" xml:id="cr2" affiliationRef="#a1"><personName><givenNames>Ai Hua</givenNames><familyName>Li</familyName></personName></creator><creator creatorRole="author" xml:id="cr3" affiliationRef="#a3"><personName><givenNames>Dong Ren</givenNames><familyName>Zhou</familyName></personName></creator><creator creatorRole="author" xml:id="cr4" affiliationRef="#a4"><personName><givenNames>Zhou Rui</givenNames><familyName>Wen</familyName></personName></creator><creator creatorRole="author" xml:id="cr5" affiliationRef="#a3"><personName><givenNames>Xue Ping</givenNames><familyName>Ye</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="a1" countryCode="CN"><unparsedAffiliation>Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China</unparsedAffiliation></affiliation><affiliation xml:id="a2" countryCode="CN"><unparsedAffiliation>Graduate University of Chinese Academy of Sciences, Beijing, China</unparsedAffiliation></affiliation><affiliation xml:id="a3" countryCode="CN"><unparsedAffiliation>Zhejiang Institute of Freshwater Fisheries, Huzhou Zhejiang, China</unparsedAffiliation></affiliation><affiliation xml:id="a4" countryCode="CN"><unparsedAffiliation>Hubei Fisheries Science Research Institute, Wuhan, China</unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en"><keyword xml:id="k1">isolation</keyword><keyword xml:id="k2">characterization</keyword><keyword xml:id="k3"><i>Edwardsiella ictaluri</i></keyword><keyword xml:id="k4">yellow catfish</keyword></keywordGroup><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Abstract</title><p>Yellow catfish <i>Pelteobagrus fulvidraco</i> (Richardson) is a commercially important fish generally distributed in Southeast Asian countries. The well‐known aetiological agent of enteric septicaemia of catfish, <i>Edwardsiella ictaluri</i>, was isolated from diseased yellow catfish <i>P. fulvidraco</i> (Richardson) reared at two commercial fisheries in China. The economic losses due to the high mortalities (about 50%) caused by this bacterium have been increasing annually. The affected fish presented two different, typical symptoms: pale gills, slight exophthalmia and a ‘hole in the head’, and haemorrhage on the opercula, in the skin under the jaw, creating a ‘hole under the jaw’. These diseases were found frequently in cultured yellow catfish throughout China. The isolates from both outbreaks were all Gram negative, facultatively anaerobic and short rod. Morphological and biochemical tests and phylogenetic analysis based on the 16S rDNA sequences all strongly indicated that these yellow catfish isolates were highly identical to the known <i>E. ictaluri</i>. In addition, the isolates possessed the typical plasmid profile of <i>E. ictaluri</i>. Experimental infection assays were conducted and pathogenicity (by an intraperitoneal injection) was demonstrated in yellow catfish and channel catfish <i>Ictalurus punctatus</i>. The results showed that yellow catfish isolates were quite conservative phenotypically and genetically, and were able to cause two different, typical symptoms in this fish under unknown conditions and mechanism.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Isolation and characterization of Edwardsiella ictaluri strains as pathogens from diseased yellow catfish Pelteobagrus fulvidraco (Richardson) cultured in China</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>E. ictaluri isolates from diseased yellow catfish</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Isolation and characterization of Edwardsiella ictaluri strains as pathogens from diseased yellow catfish Pelteobagrus fulvidraco (Richardson) cultured in China</title></titleInfo><name type="personal"><namePart type="given">Jin Yu</namePart><namePart type="family">Liu</namePart><affiliation>Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China</affiliation><affiliation>Graduate University of Chinese Academy of Sciences, Beijing, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Ai Hua</namePart><namePart type="family">Li</namePart><affiliation>Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Dong Ren</namePart><namePart type="family">Zhou</namePart><affiliation>Zhejiang Institute of Freshwater Fisheries, Huzhou Zhejiang, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Zhou Rui</namePart><namePart type="family">Wen</namePart><affiliation>Hubei Fisheries Science Research Institute, Wuhan, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Xue Ping</namePart><namePart type="family">Ye</namePart><affiliation>Zhejiang Institute of Freshwater Fisheries, Huzhou Zhejiang, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Blackwell Publishing Ltd</publisher><place><placeTerm type="text">Oxford, UK</placeTerm></place><dateIssued encoding="w3cdtf">2010-11</dateIssued><copyrightDate encoding="w3cdtf">2010</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">3</extent><extent unit="tables">3</extent><extent unit="references">50</extent><extent unit="words">7485</extent></physicalDescription><abstract lang="en">Yellow catfish Pelteobagrus fulvidraco (Richardson) is a commercially important fish generally distributed in Southeast Asian countries. The well‐known aetiological agent of enteric septicaemia of catfish, Edwardsiella ictaluri, was isolated from diseased yellow catfish P. fulvidraco (Richardson) reared at two commercial fisheries in China. The economic losses due to the high mortalities (about 50%) caused by this bacterium have been increasing annually. The affected fish presented two different, typical symptoms: pale gills, slight exophthalmia and a ‘hole in the head’, and haemorrhage on the opercula, in the skin under the jaw, creating a ‘hole under the jaw’. These diseases were found frequently in cultured yellow catfish throughout China. The isolates from both outbreaks were all Gram negative, facultatively anaerobic and short rod. Morphological and biochemical tests and phylogenetic analysis based on the 16S rDNA sequences all strongly indicated that these yellow catfish isolates were highly identical to the known E. ictaluri. In addition, the isolates possessed the typical plasmid profile of E. ictaluri. Experimental infection assays were conducted and pathogenicity (by an intraperitoneal injection) was demonstrated in yellow catfish and channel catfish Ictalurus punctatus. The results showed that yellow catfish isolates were quite conservative phenotypically and genetically, and were able to cause two different, typical symptoms in this fish under unknown conditions and mechanism.</abstract><subject lang="en"><genre>keywords</genre><topic>isolation</topic><topic>characterization</topic><topic>Edwardsiella ictaluri</topic><topic>yellow catfish</topic></subject><relatedItem type="host"><titleInfo><title>Aquaculture Research</title></titleInfo><genre type="journal">journal</genre><identifier type="ISSN">1355-557X</identifier><identifier type="eISSN">1365-2109</identifier><identifier type="DOI">10.1111/(ISSN)1365-2109</identifier><identifier type="PublisherID">ARE</identifier><part><date>2010</date><detail type="volume"><caption>vol.</caption><number>41</number></detail><detail type="issue"><caption>no.</caption><number>12</number></detail><extent unit="pages"><start>1835</start><end>1844</end><total>10</total></extent></part></relatedItem><identifier type="istex">C5ED5CDAA6121358424AB4F96459BCEE2202199E</identifier><identifier type="DOI">10.1111/j.1365-2109.2010.02571.x</identifier><identifier type="ArticleID">ARE2571</identifier><accessCondition type="use and reproduction" contentType="copyright">© 2010 The Authors. 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