Effects of nonylphenol ethoxylate exposure on reproductive output and bioindicators of environmental estrogen exposure in fathead minnows, Pimephales promelas
Identifieur interne : 000F50 ( Istex/Corpus ); précédent : 000F49; suivant : 000F51Effects of nonylphenol ethoxylate exposure on reproductive output and bioindicators of environmental estrogen exposure in fathead minnows, Pimephales promelas
Auteurs : Krista M. Nichols ; Erin M. Snyder ; Shane A. Snyder ; Susan L. Pierens ; Stephanie R. Miles-Richardson ; John P. GiesySource :
- Environmental Toxicology and Chemistry [ 0730-7268 ] ; 2001-03.
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- KwdEn :
Abstract
Nonylphenol ethoxylates (NPEOs) were evaluated in the laboratory for potential effects on the reproductive physiology and fecundity of fathead minnows (Pimephales promelas). Groups of three adult male and three female fathead minnows were exposed in a continuous flow‐through system to 0, 0.21, 0.65, 2.1, or 7.9 μg NPEO/L for 42 d. Rabbit anti‐goldfish vitellogenin (VTG) antiserum was prepared and a competitive enzyme‐linked immunosorbent assay (ELISA) was adapted for measurement of plasma VTG in fish following exposure. Plasma 17β‐estradiol (E2) and testosterone (T) were also quantified by ELISA at the end of the exposure. Neither survival nor fecundity of fathead minnows exhibited a concentration‐dependent response to NPEOs. No significant differences were observed in plasma VTG concentrations among treatments for males or females. Mean plasma VTG concentrations in females ranged from 291.7 to 895.1 μ VTG/ml among treatments and did not overlap with mean concentrations measured in the plasma of males, which ranged from less than the method detection limit (0.27 μg VTG/ml) to 3.2 μg VTG/ml. Plasma E2 concentrations exhibited a significant difference between males and females within all NPEO treatments, but no differences were observed among treatments. Similarly, plasma T concentrations did not exhibit a concentration‐dependent response to NPEOs.
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DOI: 10.1002/etc.5620200309
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Groups of three adult male and three female fathead minnows were exposed in a continuous flow‐through system to 0, 0.21, 0.65, 2.1, or 7.9 μg NPEO/L for 42 d. Rabbit anti‐goldfish vitellogenin (VTG) antiserum was prepared and a competitive enzyme‐linked immunosorbent assay (ELISA) was adapted for measurement of plasma VTG in fish following exposure. Plasma 17β‐estradiol (E2) and testosterone (T) were also quantified by ELISA at the end of the exposure. Neither survival nor fecundity of fathead minnows exhibited a concentration‐dependent response to NPEOs. No significant differences were observed in plasma VTG concentrations among treatments for males or females. Mean plasma VTG concentrations in females ranged from 291.7 to 895.1 μ VTG/ml among treatments and did not overlap with mean concentrations measured in the plasma of males, which ranged from less than the method detection limit (0.27 μg VTG/ml) to 3.2 μg VTG/ml. Plasma E2 concentrations exhibited a significant difference between males and females within all NPEO treatments, but no differences were observed among treatments. Similarly, plasma T concentrations did not exhibit a concentration‐dependent response to NPEOs.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>Krista M. Nichols</name><affiliations><json:string>Department of Fisheries and Wildlife, Michigan State University, East Lansing, Michigan 48824, USA</json:string></affiliations></json:item><json:item><name>Erin M. Snyder</name><affiliations><json:string>Department of Zoology, Michigan State University, East Lansing, Michigan 48824, USA</json:string><json:string>Institute of Environmental Toxicology, #National Food Safety and Toxicology Center, Michigan State University, East Lansing, Michigan 48824, USA</json:string></affiliations></json:item><json:item><name>Shane A. Snyder</name><affiliations><json:string>Department of Zoology, Michigan State University, East Lansing, Michigan 48824, USA</json:string><json:string>Institute of Environmental Toxicology, #National Food Safety and Toxicology Center, Michigan State University, East Lansing, Michigan 48824, USA</json:string></affiliations></json:item><json:item><name>Susan L. Pierens</name><affiliations><json:string>Department of Zoology, Michigan State University, East Lansing, Michigan 48824, USA</json:string><json:string>Institute of Environmental Toxicology, #National Food Safety and Toxicology Center, Michigan State University, East Lansing, Michigan 48824, USA</json:string></affiliations></json:item><json:item><name>Stephanie R. Miles‐Richardson</name><affiliations><json:string>Institute of Environmental Toxicology, #National Food Safety and Toxicology Center, Michigan State University, East Lansing, Michigan 48824, USA</json:string><json:string>Department of Pathology, #National Food Safety and Toxicology Center, Michigan State University, East Lansing, Michigan 48824, USA</json:string></affiliations></json:item><json:item><name>John P. Giesy</name><affiliations><json:string>Institute of Environmental Toxicology, #National Food Safety and Toxicology Center, Michigan State University, East Lansing, Michigan 48824, USA</json:string><json:string>Department of Pathology, #National Food Safety and Toxicology Center, Michigan State University, East Lansing, Michigan 48824, USA</json:string><json:string>National Food Safety and Toxicology Center, Michigan State University, East Lansing, Michigan 48824, USA</json:string><json:string>Institute of Environmental Toxicology, #National Food Safety and Toxicology Center, Michigan State University, East Lansing, Michigan 48824, USA</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Vitellogenin</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Hormones</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Estradiol</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Reproduction</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Endocrine</value></json:item></subject><articleId><json:string>ETC5620200309</json:string></articleId><language><json:string>eng</json:string></language><originalGenre><json:string>article</json:string></originalGenre><abstract>Nonylphenol ethoxylates (NPEOs) were evaluated in the laboratory for potential effects on the reproductive physiology and fecundity of fathead minnows (Pimephales promelas). Groups of three adult male and three female fathead minnows were exposed in a continuous flow‐through system to 0, 0.21, 0.65, 2.1, or 7.9 μg NPEO/L for 42 d. Rabbit anti‐goldfish vitellogenin (VTG) antiserum was prepared and a competitive enzyme‐linked immunosorbent assay (ELISA) was adapted for measurement of plasma VTG in fish following exposure. Plasma 17β‐estradiol (E2) and testosterone (T) were also quantified by ELISA at the end of the exposure. Neither survival nor fecundity of fathead minnows exhibited a concentration‐dependent response to NPEOs. No significant differences were observed in plasma VTG concentrations among treatments for males or females. Mean plasma VTG concentrations in females ranged from 291.7 to 895.1 μ VTG/ml among treatments and did not overlap with mean concentrations measured in the plasma of males, which ranged from less than the method detection limit (0.27 μg VTG/ml) to 3.2 μg VTG/ml. Plasma E2 concentrations exhibited a significant difference between males and females within all NPEO treatments, but no differences were observed among treatments. 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minnows, Pimephales promelas</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>Wiley Periodicals, Inc.</publisher><pubPlace>Hoboken</pubPlace><availability><p>Copyright © 2001 SETAC</p></availability><date>2001</date></publicationStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Effects of nonylphenol ethoxylate exposure on reproductive output and bioindicators of environmental estrogen exposure in fathead minnows, Pimephales promelas</title><author xml:id="author-1"><persName><forename type="first">Krista M.</forename><surname>Nichols</surname></persName><affiliation>Department of Fisheries and Wildlife, Michigan State University, East Lansing, Michigan 48824, USA</affiliation></author><author xml:id="author-2"><persName><forename type="first">Erin M.</forename><surname>Snyder</surname></persName><affiliation>Department of Zoology, Michigan State University, East Lansing, Michigan 48824, 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Groups of three adult male and three female fathead minnows were exposed in a continuous flow‐through system to 0, 0.21, 0.65, 2.1, or 7.9 μg NPEO/L for 42 d. Rabbit anti‐goldfish vitellogenin (VTG) antiserum was prepared and a competitive enzyme‐linked immunosorbent assay (ELISA) was adapted for measurement of plasma VTG in fish following exposure. Plasma 17β‐estradiol (E2) and testosterone (T) were also quantified by ELISA at the end of the exposure. Neither survival nor fecundity of fathead minnows exhibited a concentration‐dependent response to NPEOs. No significant differences were observed in plasma VTG concentrations among treatments for males or females. Mean plasma VTG concentrations in females ranged from 291.7 to 895.1 μ VTG/ml among treatments and did not overlap with mean concentrations measured in the plasma of males, which ranged from less than the method detection limit (0.27 μg VTG/ml) to 3.2 μg VTG/ml. Plasma E2 concentrations exhibited a significant difference between males and females within all NPEO treatments, but no differences were observed among treatments. 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Chemistry</title><title type="subtitle">An International Journal</title><title type="short">Environmental Toxicology and Chemistry</title></titleGroup></publicationMeta><publicationMeta level="part" position="30"><doi origin="wiley" registered="no">10.1002/etc.v20:3</doi><numberingGroup><numbering type="journalVolume" number="20">20</numbering><numbering type="journalIssue">3</numbering></numberingGroup><coverDate startDate="2001-03">March 2001</coverDate></publicationMeta><publicationMeta level="unit" type="article" position="9" status="forIssue"><doi origin="wiley" registered="no">10.1002/etc.5620200309</doi><idGroup><id type="unit" value="ETC5620200309"></id></idGroup><countGroup><count type="pageTotal" number="13"></count></countGroup><titleGroup><title type="articleCategory">Environmental Toxicology</title><title type="tocHeading1">Environmental Toxicology</title></titleGroup><copyright ownership="thirdParty">Copyright © 2001 SETAC</copyright><eventGroup><event 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href="file:ETC.ETC5620200309.pdf"></link></linkGroup></publicationMeta><contentMeta><countGroup><count type="figureTotal" number="7"></count><count type="tableTotal" number="4"></count><count type="referenceTotal" number="69"></count></countGroup><titleGroup><title type="main" xml:lang="en">Effects of nonylphenol ethoxylate exposure on reproductive output and bioindicators of environmental estrogen exposure in fathead minnows, <i>Pimephales promelas</i></title><title type="short" xml:lang="en">NPEO effects on fathead minnows</title></titleGroup><creators><creator xml:id="au1" creatorRole="author" affiliationRef="#af1"><personName><givenNames>Krista M.</givenNames><familyName>Nichols</familyName></personName></creator><creator xml:id="au2" creatorRole="author" affiliationRef="#af2 #af3"><personName><givenNames>Erin M.</givenNames><familyName>Snyder</familyName></personName></creator><creator xml:id="au3" creatorRole="author" affiliationRef="#af2 #af3"><personName><givenNames>Shane A.</givenNames><familyName>Snyder</familyName></personName></creator><creator xml:id="au4" creatorRole="author" affiliationRef="#af2 #af3"><personName><givenNames>Susan L.</givenNames><familyName>Pierens</familyName></personName></creator><creator xml:id="au5" creatorRole="author" affiliationRef="#af3 #af4"><personName><givenNames>Stephanie R.</givenNames><familyName>Miles‐Richardson</familyName></personName></creator><creator xml:id="au6" creatorRole="author" affiliationRef="#af3 #af4 #af5" corresponding="yes"><personName><givenNames>John P.</givenNames><familyName>Giesy</familyName></personName><contactDetails><email>jgiesy@aol.com</email></contactDetails></creator></creators><affiliationGroup><affiliation xml:id="af1" countryCode="US" type="organization"><unparsedAffiliation>Department of Fisheries and Wildlife, Michigan State University, East Lansing, Michigan 48824, USA</unparsedAffiliation></affiliation><affiliation xml:id="af2" countryCode="US" type="organization"><unparsedAffiliation>Department of Zoology, Michigan State University, East Lansing, Michigan 48824, USA</unparsedAffiliation></affiliation><affiliation xml:id="af3" countryCode="US" type="organization"><unparsedAffiliation>Institute of Environmental Toxicology, #National Food Safety and Toxicology Center, Michigan State University, East Lansing, Michigan 48824, USA</unparsedAffiliation></affiliation><affiliation xml:id="af4" countryCode="US" type="organization"><unparsedAffiliation>Department of Pathology, #National Food Safety and Toxicology Center, Michigan State University, East Lansing, Michigan 48824, USA</unparsedAffiliation></affiliation><affiliation xml:id="af5" countryCode="US" type="organization"><unparsedAffiliation>National Food Safety and Toxicology Center, Michigan State University, East Lansing, Michigan 48824, USA</unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en" type="author"><keyword xml:id="kwd1">Vitellogenin</keyword><keyword xml:id="kwd2">Hormones</keyword><keyword xml:id="kwd3">Estradiol</keyword><keyword xml:id="kwd4">Reproduction</keyword><keyword xml:id="kwd5">Endocrine</keyword></keywordGroup><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Abstract</title><p>Nonylphenol ethoxylates (NPEOs) were evaluated in the laboratory for potential effects on the reproductive physiology and fecundity of fathead minnows (<i>Pimephales promelas</i>). Groups of three adult male and three female fathead minnows were exposed in a continuous flow‐through system to 0, 0.21, 0.65, 2.1, or 7.9 μg NPEO/L for 42 d. Rabbit anti‐goldfish vitellogenin (VTG) antiserum was prepared and a competitive enzyme‐linked immunosorbent assay (ELISA) was adapted for measurement of plasma VTG in fish following exposure. Plasma 17β‐estradiol (E2) and testosterone (T) were also quantified by ELISA at the end of the exposure. Neither survival nor fecundity of fathead minnows exhibited a concentration‐dependent response to NPEOs. No significant differences were observed in plasma VTG concentrations among treatments for males or females. Mean plasma VTG concentrations in females ranged from 291.7 to 895.1 μ VTG/ml among treatments and did not overlap with mean concentrations measured in the plasma of males, which ranged from less than the method detection limit (0.27 μg VTG/ml) to 3.2 μg VTG/ml. Plasma E2 concentrations exhibited a significant difference between males and females within all NPEO treatments, but no differences were observed among treatments. Similarly, plasma T concentrations did not exhibit a concentration‐dependent response to NPEOs.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Effects of nonylphenol ethoxylate exposure on reproductive output and bioindicators of environmental estrogen exposure in fathead minnows, Pimephales promelas</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>NPEO effects on fathead minnows</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Effects of nonylphenol ethoxylate exposure on reproductive output and bioindicators of environmental estrogen exposure in fathead minnows, Pimephales promelas</title></titleInfo><name type="personal"><namePart type="given">Krista M.</namePart><namePart type="family">Nichols</namePart><affiliation>Department of Fisheries and Wildlife, Michigan State University, East Lansing, Michigan 48824, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Erin M.</namePart><namePart type="family">Snyder</namePart><affiliation>Department of Zoology, Michigan State University, East Lansing, Michigan 48824, USA</affiliation><affiliation>Institute of Environmental Toxicology, #National Food Safety and Toxicology Center, Michigan State University, East Lansing, Michigan 48824, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Shane A.</namePart><namePart type="family">Snyder</namePart><affiliation>Department of Zoology, Michigan State University, East Lansing, Michigan 48824, USA</affiliation><affiliation>Institute of Environmental Toxicology, #National Food Safety and Toxicology Center, Michigan State University, East Lansing, Michigan 48824, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Susan L.</namePart><namePart type="family">Pierens</namePart><affiliation>Department of Zoology, Michigan State University, East Lansing, Michigan 48824, USA</affiliation><affiliation>Institute of Environmental Toxicology, #National Food Safety and Toxicology Center, Michigan State University, East Lansing, Michigan 48824, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Stephanie R.</namePart><namePart type="family">Miles‐Richardson</namePart><affiliation>Institute of Environmental Toxicology, #National Food Safety and Toxicology Center, Michigan State University, East Lansing, Michigan 48824, USA</affiliation><affiliation>Department of Pathology, #National Food Safety and Toxicology Center, Michigan State University, East Lansing, Michigan 48824, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">John P.</namePart><namePart type="family">Giesy</namePart><affiliation>Institute of Environmental Toxicology, #National Food Safety and Toxicology Center, Michigan State University, East Lansing, Michigan 48824, USA</affiliation><affiliation>Department of Pathology, #National Food Safety and Toxicology Center, Michigan State University, East Lansing, Michigan 48824, USA</affiliation><affiliation>National Food Safety and Toxicology Center, Michigan State University, East Lansing, Michigan 48824, USA</affiliation><affiliation>Institute of Environmental Toxicology, #National Food Safety and Toxicology Center, Michigan State University, East Lansing, Michigan 48824, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Wiley Periodicals, Inc.</publisher><place><placeTerm type="text">Hoboken</placeTerm></place><dateIssued encoding="w3cdtf">2001-03</dateIssued><dateCaptured encoding="w3cdtf">2000-01-18</dateCaptured><dateValid encoding="w3cdtf">2000-06-25</dateValid><copyrightDate encoding="w3cdtf">2001</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">7</extent><extent unit="tables">4</extent><extent unit="references">69</extent></physicalDescription><abstract lang="en">Nonylphenol ethoxylates (NPEOs) were evaluated in the laboratory for potential effects on the reproductive physiology and fecundity of fathead minnows (Pimephales promelas). Groups of three adult male and three female fathead minnows were exposed in a continuous flow‐through system to 0, 0.21, 0.65, 2.1, or 7.9 μg NPEO/L for 42 d. Rabbit anti‐goldfish vitellogenin (VTG) antiserum was prepared and a competitive enzyme‐linked immunosorbent assay (ELISA) was adapted for measurement of plasma VTG in fish following exposure. Plasma 17β‐estradiol (E2) and testosterone (T) were also quantified by ELISA at the end of the exposure. Neither survival nor fecundity of fathead minnows exhibited a concentration‐dependent response to NPEOs. No significant differences were observed in plasma VTG concentrations among treatments for males or females. Mean plasma VTG concentrations in females ranged from 291.7 to 895.1 μ VTG/ml among treatments and did not overlap with mean concentrations measured in the plasma of males, which ranged from less than the method detection limit (0.27 μg VTG/ml) to 3.2 μg VTG/ml. Plasma E2 concentrations exhibited a significant difference between males and females within all NPEO treatments, but no differences were observed among treatments. Similarly, plasma T concentrations did not exhibit a concentration‐dependent response to NPEOs.</abstract><subject lang="en"><genre>keywords</genre><topic>Vitellogenin</topic><topic>Hormones</topic><topic>Estradiol</topic><topic>Reproduction</topic><topic>Endocrine</topic></subject><relatedItem type="host"><titleInfo><title>Environmental Toxicology and Chemistry</title><subTitle>An International Journal</subTitle></titleInfo><titleInfo type="abbreviated"><title>Environmental Toxicology and Chemistry</title></titleInfo><genre type="journal">journal</genre><subject><genre>article-category</genre><topic>Environmental Toxicology</topic></subject><identifier type="ISSN">0730-7268</identifier><identifier type="eISSN">1552-8618</identifier><identifier type="DOI">10.1002/(ISSN)1552-8618</identifier><identifier type="PublisherID">ETC</identifier><part><date>2001</date><detail type="volume"><caption>vol.</caption><number>20</number></detail><detail type="issue"><caption>no.</caption><number>3</number></detail><extent unit="pages"><start>510</start><end>522</end><total>13</total></extent></part></relatedItem><identifier type="istex">D3D67032C9B4CBF9F048C35AAF81C4D87BF5415A</identifier><identifier type="DOI">10.1002/etc.5620200309</identifier><identifier type="ArticleID">ETC5620200309</identifier><accessCondition type="use and reproduction" contentType="copyright">Copyright © 2001 SETAC</accessCondition><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>Wiley Periodicals, Inc.</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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