Streptococcus agalactiae, an Emerging Pathogen for Cultured Ya‐Fish, Schizothorax prenanti, in China
Identifieur interne : 000F08 ( Istex/Corpus ); précédent : 000F07; suivant : 000F09Streptococcus agalactiae, an Emerging Pathogen for Cultured Ya‐Fish, Schizothorax prenanti, in China
Auteurs : Y. Geng ; K. Y. Wang ; X. L. Huang ; D. F. Chen ; C. W. Li ; S. Y. Ren ; Y. T. Liao ; Z. Y. Zhou ; Q. F. Liu ; Z. J. Du ; W. M. LaiSource :
- Transboundary and Emerging Diseases [ 1865-1674 ] ; 2012-08.
English descriptors
Abstract
Streptococcus agalactiae (Group B streptococcus) has emerged as an important pathogen that affects humans and animals, including aquatic species. S. agalactiae infections are becoming an increasing problem in aquaculture and have been reported worldwide in a variety of fish species, especially those living in warm water. Recently, a very serious infectious disease of unknown aetiology broke out in ya‐fish (Schizothorax prenanti) farms in Sichuan Province. A Gram‐positive, chain‐forming coccus was isolated from moribund cultured ya‐fish. The goals of this study were to identify the bacterial strains isolated from diseased fish between 2009 and 2011 in Sichuan Province, China, to evaluate the pathogenicity of the pathogen in ya‐fish, crucian carp (Carassius carassius) and the Nile tilapia (Oreochromis niloticus); and to determine the susceptibility of the pathogen strains to many currently available anti‐microbial agents. The virulence tests were conducted by intraperitoneal injection of bacterial suspensions. In this study, four strains of a Gram‐positive, chain‐forming coccus were isolated from moribund cultured ya‐fish (S. prenanti). The coccoid microorganism was identified as S. agalactiae using a commercial streptococcal grouping kit and 16S rDNA sequencing analysis. Susceptibility of the isolates to 22 antibiotics was tested using the disc diffusion method. All isolates showed a similar antibiotic susceptibility, which were sensitive to amoxicillin, ciprofloxacin, lomefloxacin, chloramphenicol, rifampin, vancomycin, azithromycin, florfenicol, cefalexin, cefradine and deoxycycline and resistant to gentamicin, sinomin (SMZ/TMP), penicillin, tenemycin, fradiomycin and streptomycin. Furthermore, the virulence tests were conducted by intraperitoneal injection of the isolated strain GY101 in ya‐fish, crucian carp and the Nile tilapia. This coccus was lethal to ya‐fish, Nile tilapia and crucian carp. The mortality rates of infected ya‐fish were 100%, 100%, 60% and 20% at doses of 1.0 × 107, 1.0 × 106, 1.0 × 105 and 1.0 × 104 CFU/fish, respectively. The values were 100%, 80%, 60% and 30% for Nile tilapia and 100%, 60%, 10% and 0% for crucian carp. To our knowledge, this is the first report of S. agalactiae as a pathogen of ya‐fish.
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DOI: 10.1111/j.1865-1682.2011.01280.x
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Geng</name><affiliation><mods:affiliation> College of Veterinary Medicine, Sichuan Agricultural University, Ya’an, Sichuan, China</mods:affiliation></affiliation><affiliation><mods:affiliation> Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Ya’an, Sichuan, China</mods:affiliation></affiliation></author><author><name sortKey="Wang, K Y" sort="Wang, K Y" uniqKey="Wang K" first="K. Y." last="Wang">K. Y. Wang</name><affiliation><mods:affiliation> College of Veterinary Medicine, Sichuan Agricultural University, Ya’an, Sichuan, China</mods:affiliation></affiliation><affiliation><mods:affiliation> Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Ya’an, Sichuan, China</mods:affiliation></affiliation></author><author><name sortKey="Huang, X L" sort="Huang, X L" uniqKey="Huang X" first="X. L." last="Huang">X. L. Huang</name><affiliation><mods:affiliation> Department of Aquaculture, Sichuan Agricultural University, Ya’an Sichuan, China</mods:affiliation></affiliation></author><author><name sortKey="Chen, D F" sort="Chen, D F" uniqKey="Chen D" first="D. F." last="Chen">D. F. Chen</name><affiliation><mods:affiliation> Department of Aquaculture, Sichuan Agricultural University, Ya’an Sichuan, China</mods:affiliation></affiliation></author><author><name sortKey="Li, C W" sort="Li, C W" uniqKey="Li C" first="C. W." last="Li">C. W. Li</name><affiliation><mods:affiliation> College of Veterinary Medicine, Sichuan Agricultural University, Ya’an, Sichuan, China</mods:affiliation></affiliation></author><author><name sortKey="Ren, S Y" sort="Ren, S Y" uniqKey="Ren S" first="S. Y." last="Ren">S. Y. 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Liu</name><affiliation><mods:affiliation> College of Veterinary Medicine, Sichuan Agricultural University, Ya’an, Sichuan, China</mods:affiliation></affiliation></author><author><name sortKey="Du, Z J" sort="Du, Z J" uniqKey="Du Z" first="Z. J." last="Du">Z. J. Du</name><affiliation><mods:affiliation> Department of Aquaculture, Sichuan Agricultural University, Ya’an Sichuan, China</mods:affiliation></affiliation></author><author><name sortKey="Lai, W M" sort="Lai, W M" uniqKey="Lai W" first="W. M." last="Lai">W. M. 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S. agalactiae infections are becoming an increasing problem in aquaculture and have been reported worldwide in a variety of fish species, especially those living in warm water. Recently, a very serious infectious disease of unknown aetiology broke out in ya‐fish (Schizothorax prenanti) farms in Sichuan Province. A Gram‐positive, chain‐forming coccus was isolated from moribund cultured ya‐fish. The goals of this study were to identify the bacterial strains isolated from diseased fish between 2009 and 2011 in Sichuan Province, China, to evaluate the pathogenicity of the pathogen in ya‐fish, crucian carp (Carassius carassius) and the Nile tilapia (Oreochromis niloticus); and to determine the susceptibility of the pathogen strains to many currently available anti‐microbial agents. The virulence tests were conducted by intraperitoneal injection of bacterial suspensions. In this study, four strains of a Gram‐positive, chain‐forming coccus were isolated from moribund cultured ya‐fish (S. prenanti). The coccoid microorganism was identified as S. agalactiae using a commercial streptococcal grouping kit and 16S rDNA sequencing analysis. Susceptibility of the isolates to 22 antibiotics was tested using the disc diffusion method. All isolates showed a similar antibiotic susceptibility, which were sensitive to amoxicillin, ciprofloxacin, lomefloxacin, chloramphenicol, rifampin, vancomycin, azithromycin, florfenicol, cefalexin, cefradine and deoxycycline and resistant to gentamicin, sinomin (SMZ/TMP), penicillin, tenemycin, fradiomycin and streptomycin. Furthermore, the virulence tests were conducted by intraperitoneal injection of the isolated strain GY101 in ya‐fish, crucian carp and the Nile tilapia. This coccus was lethal to ya‐fish, Nile tilapia and crucian carp. The mortality rates of infected ya‐fish were 100%, 100%, 60% and 20% at doses of 1.0 × 107, 1.0 × 106, 1.0 × 105 and 1.0 × 104 CFU/fish, respectively. The values were 100%, 80%, 60% and 30% for Nile tilapia and 100%, 60%, 10% and 0% for crucian carp. To our knowledge, this is the first report of S. agalactiae as a pathogen of ya‐fish.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>Y. Geng</name><affiliations><json:string>College of Veterinary Medicine, Sichuan Agricultural University, Ya’an, Sichuan, China</json:string><json:string>Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Ya’an, Sichuan, China</json:string></affiliations></json:item><json:item><name>K. Y. Wang</name><affiliations><json:string>College of Veterinary Medicine, Sichuan Agricultural University, Ya’an, Sichuan, China</json:string><json:string>Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Ya’an, Sichuan, China</json:string></affiliations></json:item><json:item><name>X. L. Huang</name><affiliations><json:string>Department of Aquaculture, Sichuan Agricultural University, Ya’an Sichuan, China</json:string></affiliations></json:item><json:item><name>D. F. Chen</name><affiliations><json:string>Department of Aquaculture, Sichuan Agricultural University, Ya’an Sichuan, China</json:string></affiliations></json:item><json:item><name>C. W. Li</name><affiliations><json:string>College of Veterinary Medicine, Sichuan Agricultural University, Ya’an, Sichuan, China</json:string></affiliations></json:item><json:item><name>S. Y. Ren</name><affiliations><json:string>College of Veterinary Medicine, Sichuan Agricultural University, Ya’an, Sichuan, China</json:string></affiliations></json:item><json:item><name>Y. T. Liao</name><affiliations><json:string>College of Veterinary Medicine, Sichuan Agricultural University, Ya’an, Sichuan, China</json:string></affiliations></json:item><json:item><name>Z. Y. Zhou</name><affiliations><json:string>College of Veterinary Medicine, Sichuan Agricultural University, Ya’an, Sichuan, China</json:string></affiliations></json:item><json:item><name>Q. F. Liu</name><affiliations><json:string>College of Veterinary Medicine, Sichuan Agricultural University, Ya’an, Sichuan, China</json:string></affiliations></json:item><json:item><name>Z. J. Du</name><affiliations><json:string>Department of Aquaculture, Sichuan Agricultural University, Ya’an Sichuan, China</json:string></affiliations></json:item><json:item><name>W. M. 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S. agalactiae infections are becoming an increasing problem in aquaculture and have been reported worldwide in a variety of fish species, especially those living in warm water. Recently, a very serious infectious disease of unknown aetiology broke out in ya‐fish (Schizothorax prenanti) farms in Sichuan Province. A Gram‐positive, chain‐forming coccus was isolated from moribund cultured ya‐fish. The goals of this study were to identify the bacterial strains isolated from diseased fish between 2009 and 2011 in Sichuan Province, China, to evaluate the pathogenicity of the pathogen in ya‐fish, crucian carp (Carassius carassius) and the Nile tilapia (Oreochromis niloticus); and to determine the susceptibility of the pathogen strains to many currently available anti‐microbial agents. The virulence tests were conducted by intraperitoneal injection of bacterial suspensions. In this study, four strains of a Gram‐positive, chain‐forming coccus were isolated from moribund cultured ya‐fish (S. prenanti). The coccoid microorganism was identified as S. agalactiae using a commercial streptococcal grouping kit and 16S rDNA sequencing analysis. Susceptibility of the isolates to 22 antibiotics was tested using the disc diffusion method. All isolates showed a similar antibiotic susceptibility, which were sensitive to amoxicillin, ciprofloxacin, lomefloxacin, chloramphenicol, rifampin, vancomycin, azithromycin, florfenicol, cefalexin, cefradine and deoxycycline and resistant to gentamicin, sinomin (SMZ/TMP), penicillin, tenemycin, fradiomycin and streptomycin. Furthermore, the virulence tests were conducted by intraperitoneal injection of the isolated strain GY101 in ya‐fish, crucian carp and the Nile tilapia. This coccus was lethal to ya‐fish, Nile tilapia and crucian carp. The mortality rates of infected ya‐fish were 100%, 100%, 60% and 20% at doses of 1.0 × 107, 1.0 × 106, 1.0 × 105 and 1.0 × 104 CFU/fish, respectively. The values were 100%, 80%, 60% and 30% for Nile tilapia and 100%, 60%, 10% and 0% for crucian carp. 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Y.</forename><surname>Wang</surname></persName><affiliation> College of Veterinary Medicine, Sichuan Agricultural University, Ya’an, Sichuan, China</affiliation><affiliation> Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Ya’an, Sichuan, China</affiliation></author><author xml:id="author-3"><persName><forename type="first">X. L.</forename><surname>Huang</surname></persName><affiliation> Department of Aquaculture, Sichuan Agricultural University, Ya’an Sichuan, China</affiliation></author><author xml:id="author-4"><persName><forename type="first">D. F.</forename><surname>Chen</surname></persName><affiliation> Department of Aquaculture, Sichuan Agricultural University, Ya’an Sichuan, China</affiliation></author><author xml:id="author-5"><persName><forename type="first">C. W.</forename><surname>Li</surname></persName><affiliation> College of Veterinary Medicine, Sichuan Agricultural University, Ya’an, Sichuan, China</affiliation></author><author xml:id="author-6"><persName><forename type="first">S. Y.</forename><surname>Ren</surname></persName><affiliation> College of Veterinary Medicine, Sichuan Agricultural University, Ya’an, Sichuan, China</affiliation></author><author xml:id="author-7"><persName><forename type="first">Y. T.</forename><surname>Liao</surname></persName><affiliation> College of Veterinary Medicine, Sichuan Agricultural University, Ya’an, Sichuan, China</affiliation></author><author xml:id="author-8"><persName><forename type="first">Z. Y.</forename><surname>Zhou</surname></persName><affiliation> College of Veterinary Medicine, Sichuan Agricultural University, Ya’an, Sichuan, China</affiliation></author><author xml:id="author-9"><persName><forename type="first">Q. F.</forename><surname>Liu</surname></persName><affiliation> College of Veterinary Medicine, Sichuan Agricultural University, Ya’an, Sichuan, China</affiliation></author><author xml:id="author-10"><persName><forename type="first">Z. J.</forename><surname>Du</surname></persName><affiliation> Department of Aquaculture, Sichuan Agricultural University, Ya’an Sichuan, China</affiliation></author><author xml:id="author-11"><persName><forename type="first">W. M.</forename><surname>Lai</surname></persName><affiliation> College of Veterinary Medicine, Sichuan Agricultural University, Ya’an, Sichuan, China</affiliation></author></analytic><monogr><title level="j">Transboundary and Emerging Diseases</title><idno type="pISSN">1865-1674</idno><idno type="eISSN">1865-1682</idno><idno type="DOI">10.1111/(ISSN)1865-1682</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2012-08"></date><biblScope unit="volume">59</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="369">369</biblScope><biblScope unit="page" to="375">375</biblScope></imprint></monogr><idno type="istex">54D4F1AAE9E57F74DCC78548F076202545A152FE</idno><idno type="DOI">10.1111/j.1865-1682.2011.01280.x</idno><idno type="ArticleID">TBED1280</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2012</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Streptococcus agalactiae (Group B streptococcus) has emerged as an important pathogen that affects humans and animals, including aquatic species. S. agalactiae infections are becoming an increasing problem in aquaculture and have been reported worldwide in a variety of fish species, especially those living in warm water. Recently, a very serious infectious disease of unknown aetiology broke out in ya‐fish (Schizothorax prenanti) farms in Sichuan Province. A Gram‐positive, chain‐forming coccus was isolated from moribund cultured ya‐fish. The goals of this study were to identify the bacterial strains isolated from diseased fish between 2009 and 2011 in Sichuan Province, China, to evaluate the pathogenicity of the pathogen in ya‐fish, crucian carp (Carassius carassius) and the Nile tilapia (Oreochromis niloticus); and to determine the susceptibility of the pathogen strains to many currently available anti‐microbial agents. The virulence tests were conducted by intraperitoneal injection of bacterial suspensions. In this study, four strains of a Gram‐positive, chain‐forming coccus were isolated from moribund cultured ya‐fish (S. prenanti). The coccoid microorganism was identified as S. agalactiae using a commercial streptococcal grouping kit and 16S rDNA sequencing analysis. Susceptibility of the isolates to 22 antibiotics was tested using the disc diffusion method. All isolates showed a similar antibiotic susceptibility, which were sensitive to amoxicillin, ciprofloxacin, lomefloxacin, chloramphenicol, rifampin, vancomycin, azithromycin, florfenicol, cefalexin, cefradine and deoxycycline and resistant to gentamicin, sinomin (SMZ/TMP), penicillin, tenemycin, fradiomycin and streptomycin. Furthermore, the virulence tests were conducted by intraperitoneal injection of the isolated strain GY101 in ya‐fish, crucian carp and the Nile tilapia. This coccus was lethal to ya‐fish, Nile tilapia and crucian carp. The mortality rates of infected ya‐fish were 100%, 100%, 60% and 20% at doses of 1.0 × 107, 1.0 × 106, 1.0 × 105 and 1.0 × 104 CFU/fish, respectively. The values were 100%, 80%, 60% and 30% for Nile tilapia and 100%, 60%, 10% and 0% for crucian carp. To our knowledge, this is the first report of S. agalactiae as a pathogen of ya‐fish.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>keywords</head><item><term>Streptococcus agalactiae</term></item><item><term>pathogenicity</term></item><item><term>characterization</term></item><item><term>Schizothorax prenanti</term></item></list></keywords></textClass><textClass><keywords scheme="Journal Subject"><list><head>article-category</head><item><term>ORIGINAL ARTICLE</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2012-08">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/54D4F1AAE9E57F74DCC78548F076202545A152FE/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>Blackwell Publishing Ltd</publisherName><publisherLoc>Oxford, UK</publisherLoc></publisherInfo><doi origin="wiley" registered="yes">10.1111/(ISSN)1865-1682</doi><issn type="print">1865-1674</issn><issn type="electronic">1865-1682</issn><idGroup><id type="product" value="TBED"></id><id type="publisherDivision" value="ST"></id></idGroup><titleGroup><title type="main" sort="TRANSBOUNDARY AND EMERGING DISEASES">Transboundary and Emerging Diseases</title></titleGroup></publicationMeta><publicationMeta level="part" position="08104"><doi origin="wiley">10.1111/tbed.2012.59.issue-4</doi><numberingGroup><numbering type="journalVolume" number="59">59</numbering><numbering type="journalIssue" number="4">4</numbering></numberingGroup><coverDate startDate="2012-08">August 2012</coverDate></publicationMeta><publicationMeta level="unit" type="article" position="11" status="forIssue"><doi origin="wiley">10.1111/j.1865-1682.2011.01280.x</doi><idGroup><id type="unit" value="TBED1280"></id></idGroup><countGroup><count type="pageTotal" number="7"></count></countGroup><titleGroup><title type="tocHeading1">ORIGINAL ARTICLES</title><title type="articleCategory">ORIGINAL ARTICLE</title></titleGroup><copyright>© 2011 Blackwell Verlag GmbH</copyright><eventGroup><event type="xmlConverted" agent="Converter:BPG_TO_WML3G version:3.1.5.1 mode:FullText" date="2012-07-06"></event><event type="publishedOnlineEarlyUnpaginated" date="2011-12-07"></event><event type="firstOnline" date="2011-12-07"></event><event type="publishedOnlineFinalForm" date="2012-07-06"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:3.8.8" date="2014-02-10"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.3.4 mode:FullText" date="2015-02-25"></event></eventGroup><numberingGroup><numbering type="pageFirst" number="369">369</numbering><numbering type="pageLast" number="375">375</numbering></numberingGroup><correspondenceTo>Geng Yi, Xinkang Street No. 46, Yaan, Sichuan Province, China. Tel.: 860835‐2885753; Fax: 860835‐2885302; E‐mail:<email>gengyisicau@126.com</email></correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:TBED.TBED1280.pdf"></link></linkGroup></publicationMeta><contentMeta><unparsedEditorialHistory>Received for publication October 8, 2011</unparsedEditorialHistory><countGroup><count type="figureTotal" number="3"></count><count type="tableTotal" number="1"></count></countGroup><titleGroup><title type="main"><i>Streptococcus agalactiae</i>, an Emerging Pathogen for Cultured Ya‐Fish, <i>Schizothorax prenanti</i>, in China</title><title type="shortAuthors">Y. Geng et al.</title><title type="short"><i>Streptococcus agalactiae</i>, an Emerging Pathogen for Cultured Ya‐Fish</title></titleGroup><creators><creator creatorRole="author" xml:id="cr1" affiliationRef="#a1 #a2"><personName><givenNames>Y.</givenNames><familyName>Geng</familyName></personName></creator><creator creatorRole="author" xml:id="cr2" affiliationRef="#a1 #a2"><personName><givenNames>K. Y.</givenNames><familyName>Wang</familyName></personName></creator><creator creatorRole="author" xml:id="cr3" affiliationRef="#a3"><personName><givenNames>X. L.</givenNames><familyName>Huang</familyName></personName></creator><creator creatorRole="author" xml:id="cr4" affiliationRef="#a3"><personName><givenNames>D. F.</givenNames><familyName>Chen</familyName></personName></creator><creator creatorRole="author" xml:id="cr5" affiliationRef="#a1"><personName><givenNames>C. W.</givenNames><familyName>Li</familyName></personName></creator><creator creatorRole="author" xml:id="cr6" affiliationRef="#a1"><personName><givenNames>S. Y.</givenNames><familyName>Ren</familyName></personName></creator><creator creatorRole="author" xml:id="cr7" affiliationRef="#a1"><personName><givenNames>Y. T.</givenNames><familyName>Liao</familyName></personName></creator><creator creatorRole="author" xml:id="cr8" affiliationRef="#a1"><personName><givenNames>Z. Y.</givenNames><familyName>Zhou</familyName></personName></creator><creator creatorRole="author" xml:id="cr9" affiliationRef="#a1"><personName><givenNames>Q. F.</givenNames><familyName>Liu</familyName></personName></creator><creator creatorRole="author" xml:id="cr10" affiliationRef="#a3"><personName><givenNames>Z. J.</givenNames><familyName>Du</familyName></personName></creator><creator creatorRole="author" xml:id="cr11" affiliationRef="#a1"><personName><givenNames>W. M.</givenNames><familyName>Lai</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="a1" countryCode="CN"><unparsedAffiliation> College of Veterinary Medicine, Sichuan Agricultural University, Ya’an, Sichuan, China</unparsedAffiliation></affiliation><affiliation xml:id="a2" countryCode="CN"><unparsedAffiliation> Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Ya’an, Sichuan, China</unparsedAffiliation></affiliation><affiliation xml:id="a3" countryCode="CN"><unparsedAffiliation> Department of Aquaculture, Sichuan Agricultural University, Ya’an Sichuan, China</unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en"><keyword xml:id="k1"><i>Streptococcus agalactiae</i></keyword><keyword xml:id="k2">pathogenicity</keyword><keyword xml:id="k3">characterization</keyword><keyword xml:id="k4"><i>Schizothorax prenanti</i></keyword></keywordGroup><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Summary</title><p><i>Streptococcus agalactia</i>e (Group B streptococcus) has emerged as an important pathogen that affects humans and animals, including aquatic species. <i>S. agalactia</i>e infections are becoming an increasing problem in aquaculture and have been reported worldwide in a variety of fish species, especially those living in warm water. Recently, a very serious infectious disease of unknown aetiology broke out in ya‐fish (<i>Schizothorax prenanti</i>) farms in Sichuan Province. A Gram‐positive, chain‐forming coccus was isolated from moribund cultured ya‐fish. The goals of this study were to identify the bacterial strains isolated from diseased fish between 2009 and 2011 in Sichuan Province, China, to evaluate the pathogenicity of the pathogen in ya‐fish, crucian carp (<i>Carassius carassius</i>) and the Nile tilapia (<i>Oreochromis niloticus</i>); and to determine the susceptibility of the pathogen strains to many currently available anti‐microbial agents. The virulence tests were conducted by intraperitoneal injection of bacterial suspensions. In this study, four strains of a Gram‐positive, chain‐forming coccus were isolated from moribund cultured ya‐fish (<i>S. prenanti</i>). The coccoid microorganism was identified as <i>S. agalactiae</i> using a commercial streptococcal grouping kit and 16S rDNA sequencing analysis. Susceptibility of the isolates to 22 antibiotics was tested using the disc diffusion method. All isolates showed a similar antibiotic susceptibility, which were sensitive to amoxicillin, ciprofloxacin, lomefloxacin, chloramphenicol, rifampin, vancomycin, azithromycin, florfenicol, cefalexin, cefradine and deoxycycline and resistant to gentamicin, sinomin (SMZ/TMP), penicillin, tenemycin, fradiomycin and streptomycin. Furthermore, the virulence tests were conducted by intraperitoneal injection of the isolated strain GY101 in ya‐fish, crucian carp and the Nile tilapia. This coccus was lethal to ya‐fish, Nile tilapia and crucian carp. The mortality rates of infected ya‐fish were 100%, 100%, 60% and 20% at doses of 1.0 × 10<sup>7</sup>, 1.0 × 10<sup>6</sup>, 1.0 × 10<sup>5</sup> and 1.0 × 10<sup>4</sup> CFU/fish, respectively. The values were 100%, 80%, 60% and 30% for Nile tilapia and 100%, 60%, 10% and 0% for crucian carp. To our knowledge, this is the first report of <i>S. agalactiae</i> as a pathogen of ya‐fish.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Streptococcus agalactiae, an Emerging Pathogen for Cultured Ya‐Fish, Schizothorax prenanti, in China</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>Streptococcus agalactiae, an Emerging Pathogen for Cultured Ya‐Fish</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Streptococcus agalactiae, an Emerging Pathogen for Cultured Ya‐Fish, Schizothorax prenanti, in China</title></titleInfo><name type="personal"><namePart type="given">Y.</namePart><namePart type="family">Geng</namePart><affiliation> College of Veterinary Medicine, Sichuan Agricultural University, Ya’an, Sichuan, China</affiliation><affiliation> Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Ya’an, Sichuan, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">K. Y.</namePart><namePart type="family">Wang</namePart><affiliation> College of Veterinary Medicine, Sichuan Agricultural University, Ya’an, Sichuan, China</affiliation><affiliation> Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Ya’an, Sichuan, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">X. L.</namePart><namePart type="family">Huang</namePart><affiliation> Department of Aquaculture, Sichuan Agricultural University, Ya’an Sichuan, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">D. F.</namePart><namePart type="family">Chen</namePart><affiliation> Department of Aquaculture, Sichuan Agricultural University, Ya’an Sichuan, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">C. W.</namePart><namePart type="family">Li</namePart><affiliation> College of Veterinary Medicine, Sichuan Agricultural University, Ya’an, Sichuan, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S. Y.</namePart><namePart type="family">Ren</namePart><affiliation> College of Veterinary Medicine, Sichuan Agricultural University, Ya’an, Sichuan, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Y. T.</namePart><namePart type="family">Liao</namePart><affiliation> College of Veterinary Medicine, Sichuan Agricultural University, Ya’an, Sichuan, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Z. Y.</namePart><namePart type="family">Zhou</namePart><affiliation> College of Veterinary Medicine, Sichuan Agricultural University, Ya’an, Sichuan, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Q. F.</namePart><namePart type="family">Liu</namePart><affiliation> College of Veterinary Medicine, Sichuan Agricultural University, Ya’an, Sichuan, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Z. J.</namePart><namePart type="family">Du</namePart><affiliation> Department of Aquaculture, Sichuan Agricultural University, Ya’an Sichuan, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">W. M.</namePart><namePart type="family">Lai</namePart><affiliation> College of Veterinary Medicine, Sichuan Agricultural University, Ya’an, Sichuan, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Blackwell Publishing Ltd</publisher><place><placeTerm type="text">Oxford, UK</placeTerm></place><dateIssued encoding="w3cdtf">2012-08</dateIssued><edition>Received for publication October 8, 2011</edition><copyrightDate encoding="w3cdtf">2012</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">3</extent><extent unit="tables">1</extent></physicalDescription><abstract lang="en">Streptococcus agalactiae (Group B streptococcus) has emerged as an important pathogen that affects humans and animals, including aquatic species. S. agalactiae infections are becoming an increasing problem in aquaculture and have been reported worldwide in a variety of fish species, especially those living in warm water. Recently, a very serious infectious disease of unknown aetiology broke out in ya‐fish (Schizothorax prenanti) farms in Sichuan Province. A Gram‐positive, chain‐forming coccus was isolated from moribund cultured ya‐fish. The goals of this study were to identify the bacterial strains isolated from diseased fish between 2009 and 2011 in Sichuan Province, China, to evaluate the pathogenicity of the pathogen in ya‐fish, crucian carp (Carassius carassius) and the Nile tilapia (Oreochromis niloticus); and to determine the susceptibility of the pathogen strains to many currently available anti‐microbial agents. The virulence tests were conducted by intraperitoneal injection of bacterial suspensions. In this study, four strains of a Gram‐positive, chain‐forming coccus were isolated from moribund cultured ya‐fish (S. prenanti). The coccoid microorganism was identified as S. agalactiae using a commercial streptococcal grouping kit and 16S rDNA sequencing analysis. Susceptibility of the isolates to 22 antibiotics was tested using the disc diffusion method. All isolates showed a similar antibiotic susceptibility, which were sensitive to amoxicillin, ciprofloxacin, lomefloxacin, chloramphenicol, rifampin, vancomycin, azithromycin, florfenicol, cefalexin, cefradine and deoxycycline and resistant to gentamicin, sinomin (SMZ/TMP), penicillin, tenemycin, fradiomycin and streptomycin. Furthermore, the virulence tests were conducted by intraperitoneal injection of the isolated strain GY101 in ya‐fish, crucian carp and the Nile tilapia. This coccus was lethal to ya‐fish, Nile tilapia and crucian carp. The mortality rates of infected ya‐fish were 100%, 100%, 60% and 20% at doses of 1.0 × 107, 1.0 × 106, 1.0 × 105 and 1.0 × 104 CFU/fish, respectively. The values were 100%, 80%, 60% and 30% for Nile tilapia and 100%, 60%, 10% and 0% for crucian carp. To our knowledge, this is the first report of S. agalactiae as a pathogen of ya‐fish.</abstract><subject lang="en"><genre>keywords</genre><topic>Streptococcus agalactiae</topic><topic>pathogenicity</topic><topic>characterization</topic><topic>Schizothorax prenanti</topic></subject><relatedItem type="host"><titleInfo><title>Transboundary and Emerging Diseases</title></titleInfo><genre type="journal">journal</genre><subject><genre>article-category</genre><topic>ORIGINAL ARTICLE</topic></subject><identifier type="ISSN">1865-1674</identifier><identifier type="eISSN">1865-1682</identifier><identifier type="DOI">10.1111/(ISSN)1865-1682</identifier><identifier type="PublisherID">TBED</identifier><part><date>2012</date><detail type="volume"><caption>vol.</caption><number>59</number></detail><detail type="issue"><caption>no.</caption><number>4</number></detail><extent unit="pages"><start>369</start><end>375</end><total>7</total></extent></part></relatedItem><identifier type="istex">54D4F1AAE9E57F74DCC78548F076202545A152FE</identifier><identifier type="DOI">10.1111/j.1865-1682.2011.01280.x</identifier><identifier type="ArticleID">TBED1280</identifier><accessCondition type="use and reproduction" contentType="copyright">© 2011 Blackwell Verlag GmbH</accessCondition><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>Blackwell Publishing Ltd</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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