Streptococcus agalactiae, an Emerging Pathogen for Cultured Ya‐Fish, Schizothorax prenanti, in China
Identifieur interne : 000F08 ( Istex/Corpus ); précédent : 000F07; suivant : 000F09Streptococcus agalactiae, an Emerging Pathogen for Cultured Ya‐Fish, Schizothorax prenanti, in China
Auteurs : Y. Geng ; K. Y. Wang ; X. L. Huang ; D. F. Chen ; C. W. Li ; S. Y. Ren ; Y. T. Liao ; Z. Y. Zhou ; Q. F. Liu ; Z. J. Du ; W. M. LaiSource :
- Transboundary and Emerging Diseases [ 1865-1674 ] ; 2012-08.
English descriptors
Abstract
Streptococcus agalactiae (Group B streptococcus) has emerged as an important pathogen that affects humans and animals, including aquatic species. S. agalactiae infections are becoming an increasing problem in aquaculture and have been reported worldwide in a variety of fish species, especially those living in warm water. Recently, a very serious infectious disease of unknown aetiology broke out in ya‐fish (Schizothorax prenanti) farms in Sichuan Province. A Gram‐positive, chain‐forming coccus was isolated from moribund cultured ya‐fish. The goals of this study were to identify the bacterial strains isolated from diseased fish between 2009 and 2011 in Sichuan Province, China, to evaluate the pathogenicity of the pathogen in ya‐fish, crucian carp (Carassius carassius) and the Nile tilapia (Oreochromis niloticus); and to determine the susceptibility of the pathogen strains to many currently available anti‐microbial agents. The virulence tests were conducted by intraperitoneal injection of bacterial suspensions. In this study, four strains of a Gram‐positive, chain‐forming coccus were isolated from moribund cultured ya‐fish (S. prenanti). The coccoid microorganism was identified as S. agalactiae using a commercial streptococcal grouping kit and 16S rDNA sequencing analysis. Susceptibility of the isolates to 22 antibiotics was tested using the disc diffusion method. All isolates showed a similar antibiotic susceptibility, which were sensitive to amoxicillin, ciprofloxacin, lomefloxacin, chloramphenicol, rifampin, vancomycin, azithromycin, florfenicol, cefalexin, cefradine and deoxycycline and resistant to gentamicin, sinomin (SMZ/TMP), penicillin, tenemycin, fradiomycin and streptomycin. Furthermore, the virulence tests were conducted by intraperitoneal injection of the isolated strain GY101 in ya‐fish, crucian carp and the Nile tilapia. This coccus was lethal to ya‐fish, Nile tilapia and crucian carp. The mortality rates of infected ya‐fish were 100%, 100%, 60% and 20% at doses of 1.0 × 107, 1.0 × 106, 1.0 × 105 and 1.0 × 104 CFU/fish, respectively. The values were 100%, 80%, 60% and 30% for Nile tilapia and 100%, 60%, 10% and 0% for crucian carp. To our knowledge, this is the first report of S. agalactiae as a pathogen of ya‐fish.
Url:
DOI: 10.1111/j.1865-1682.2011.01280.x
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<front><div type="abstract" xml:lang="en">Streptococcus agalactiae (Group B streptococcus) has emerged as an important pathogen that affects humans and animals, including aquatic species. S. agalactiae infections are becoming an increasing problem in aquaculture and have been reported worldwide in a variety of fish species, especially those living in warm water. Recently, a very serious infectious disease of unknown aetiology broke out in ya‐fish (Schizothorax prenanti) farms in Sichuan Province. A Gram‐positive, chain‐forming coccus was isolated from moribund cultured ya‐fish. The goals of this study were to identify the bacterial strains isolated from diseased fish between 2009 and 2011 in Sichuan Province, China, to evaluate the pathogenicity of the pathogen in ya‐fish, crucian carp (Carassius carassius) and the Nile tilapia (Oreochromis niloticus); and to determine the susceptibility of the pathogen strains to many currently available anti‐microbial agents. The virulence tests were conducted by intraperitoneal injection of bacterial suspensions. In this study, four strains of a Gram‐positive, chain‐forming coccus were isolated from moribund cultured ya‐fish (S. prenanti). The coccoid microorganism was identified as S. agalactiae using a commercial streptococcal grouping kit and 16S rDNA sequencing analysis. Susceptibility of the isolates to 22 antibiotics was tested using the disc diffusion method. All isolates showed a similar antibiotic susceptibility, which were sensitive to amoxicillin, ciprofloxacin, lomefloxacin, chloramphenicol, rifampin, vancomycin, azithromycin, florfenicol, cefalexin, cefradine and deoxycycline and resistant to gentamicin, sinomin (SMZ/TMP), penicillin, tenemycin, fradiomycin and streptomycin. Furthermore, the virulence tests were conducted by intraperitoneal injection of the isolated strain GY101 in ya‐fish, crucian carp and the Nile tilapia. This coccus was lethal to ya‐fish, Nile tilapia and crucian carp. The mortality rates of infected ya‐fish were 100%, 100%, 60% and 20% at doses of 1.0 × 107, 1.0 × 106, 1.0 × 105 and 1.0 × 104 CFU/fish, respectively. The values were 100%, 80%, 60% and 30% for Nile tilapia and 100%, 60%, 10% and 0% for crucian carp. To our knowledge, this is the first report of S. agalactiae as a pathogen of ya‐fish.</div>
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<abstract>Streptococcus agalactiae (Group B streptococcus) has emerged as an important pathogen that affects humans and animals, including aquatic species. S. agalactiae infections are becoming an increasing problem in aquaculture and have been reported worldwide in a variety of fish species, especially those living in warm water. Recently, a very serious infectious disease of unknown aetiology broke out in ya‐fish (Schizothorax prenanti) farms in Sichuan Province. A Gram‐positive, chain‐forming coccus was isolated from moribund cultured ya‐fish. The goals of this study were to identify the bacterial strains isolated from diseased fish between 2009 and 2011 in Sichuan Province, China, to evaluate the pathogenicity of the pathogen in ya‐fish, crucian carp (Carassius carassius) and the Nile tilapia (Oreochromis niloticus); and to determine the susceptibility of the pathogen strains to many currently available anti‐microbial agents. The virulence tests were conducted by intraperitoneal injection of bacterial suspensions. In this study, four strains of a Gram‐positive, chain‐forming coccus were isolated from moribund cultured ya‐fish (S. prenanti). The coccoid microorganism was identified as S. agalactiae using a commercial streptococcal grouping kit and 16S rDNA sequencing analysis. Susceptibility of the isolates to 22 antibiotics was tested using the disc diffusion method. All isolates showed a similar antibiotic susceptibility, which were sensitive to amoxicillin, ciprofloxacin, lomefloxacin, chloramphenicol, rifampin, vancomycin, azithromycin, florfenicol, cefalexin, cefradine and deoxycycline and resistant to gentamicin, sinomin (SMZ/TMP), penicillin, tenemycin, fradiomycin and streptomycin. Furthermore, the virulence tests were conducted by intraperitoneal injection of the isolated strain GY101 in ya‐fish, crucian carp and the Nile tilapia. This coccus was lethal to ya‐fish, Nile tilapia and crucian carp. The mortality rates of infected ya‐fish were 100%, 100%, 60% and 20% at doses of 1.0 × 107, 1.0 × 106, 1.0 × 105 and 1.0 × 104 CFU/fish, respectively. The values were 100%, 80%, 60% and 30% for Nile tilapia and 100%, 60%, 10% and 0% for crucian carp. To our knowledge, this is the first report of S. agalactiae as a pathogen of ya‐fish.</abstract>
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<abstract xml:lang="en"><p>Streptococcus agalactiae (Group B streptococcus) has emerged as an important pathogen that affects humans and animals, including aquatic species. S. agalactiae infections are becoming an increasing problem in aquaculture and have been reported worldwide in a variety of fish species, especially those living in warm water. Recently, a very serious infectious disease of unknown aetiology broke out in ya‐fish (Schizothorax prenanti) farms in Sichuan Province. A Gram‐positive, chain‐forming coccus was isolated from moribund cultured ya‐fish. The goals of this study were to identify the bacterial strains isolated from diseased fish between 2009 and 2011 in Sichuan Province, China, to evaluate the pathogenicity of the pathogen in ya‐fish, crucian carp (Carassius carassius) and the Nile tilapia (Oreochromis niloticus); and to determine the susceptibility of the pathogen strains to many currently available anti‐microbial agents. The virulence tests were conducted by intraperitoneal injection of bacterial suspensions. In this study, four strains of a Gram‐positive, chain‐forming coccus were isolated from moribund cultured ya‐fish (S. prenanti). The coccoid microorganism was identified as S. agalactiae using a commercial streptococcal grouping kit and 16S rDNA sequencing analysis. Susceptibility of the isolates to 22 antibiotics was tested using the disc diffusion method. All isolates showed a similar antibiotic susceptibility, which were sensitive to amoxicillin, ciprofloxacin, lomefloxacin, chloramphenicol, rifampin, vancomycin, azithromycin, florfenicol, cefalexin, cefradine and deoxycycline and resistant to gentamicin, sinomin (SMZ/TMP), penicillin, tenemycin, fradiomycin and streptomycin. Furthermore, the virulence tests were conducted by intraperitoneal injection of the isolated strain GY101 in ya‐fish, crucian carp and the Nile tilapia. This coccus was lethal to ya‐fish, Nile tilapia and crucian carp. The mortality rates of infected ya‐fish were 100%, 100%, 60% and 20% at doses of 1.0 × 107, 1.0 × 106, 1.0 × 105 and 1.0 × 104 CFU/fish, respectively. The values were 100%, 80%, 60% and 30% for Nile tilapia and 100%, 60%, 10% and 0% for crucian carp. To our knowledge, this is the first report of S. agalactiae as a pathogen of ya‐fish.</p>
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<correspondenceTo>Geng Yi, Xinkang Street No. 46, Yaan, Sichuan Province, China. Tel.: 860835‐2885753; Fax: 860835‐2885302; E‐mail:<email>gengyisicau@126.com</email>
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<titleGroup><title type="main"><i>Streptococcus agalactiae</i>
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, in China</title>
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, an Emerging Pathogen for Cultured Ya‐Fish</title>
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<abstractGroup><abstract type="main" xml:lang="en"><title type="main">Summary</title>
<p><i>Streptococcus agalactia</i>
e (Group B streptococcus) has emerged as an important pathogen that affects humans and animals, including aquatic species. <i>S. agalactia</i>
e infections are becoming an increasing problem in aquaculture and have been reported worldwide in a variety of fish species, especially those living in warm water. Recently, a very serious infectious disease of unknown aetiology broke out in ya‐fish (<i>Schizothorax prenanti</i>
) farms in Sichuan Province. A Gram‐positive, chain‐forming coccus was isolated from moribund cultured ya‐fish. The goals of this study were to identify the bacterial strains isolated from diseased fish between 2009 and 2011 in Sichuan Province, China, to evaluate the pathogenicity of the pathogen in ya‐fish, crucian carp (<i>Carassius carassius</i>
) and the Nile tilapia (<i>Oreochromis niloticus</i>
); and to determine the susceptibility of the pathogen strains to many currently available anti‐microbial agents. The virulence tests were conducted by intraperitoneal injection of bacterial suspensions. In this study, four strains of a Gram‐positive, chain‐forming coccus were isolated from moribund cultured ya‐fish (<i>S. prenanti</i>
). The coccoid microorganism was identified as <i>S. agalactiae</i>
using a commercial streptococcal grouping kit and 16S rDNA sequencing analysis. Susceptibility of the isolates to 22 antibiotics was tested using the disc diffusion method. All isolates showed a similar antibiotic susceptibility, which were sensitive to amoxicillin, ciprofloxacin, lomefloxacin, chloramphenicol, rifampin, vancomycin, azithromycin, florfenicol, cefalexin, cefradine and deoxycycline and resistant to gentamicin, sinomin (SMZ/TMP), penicillin, tenemycin, fradiomycin and streptomycin. Furthermore, the virulence tests were conducted by intraperitoneal injection of the isolated strain GY101 in ya‐fish, crucian carp and the Nile tilapia. This coccus was lethal to ya‐fish, Nile tilapia and crucian carp. The mortality rates of infected ya‐fish were 100%, 100%, 60% and 20% at doses of 1.0 × 10<sup>7</sup>
, 1.0 × 10<sup>6</sup>
, 1.0 × 10<sup>5</sup>
and 1.0 × 10<sup>4</sup>
CFU/fish, respectively. The values were 100%, 80%, 60% and 30% for Nile tilapia and 100%, 60%, 10% and 0% for crucian carp. To our knowledge, this is the first report of <i>S. agalactiae</i>
as a pathogen of ya‐fish.</p>
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<abstract lang="en">Streptococcus agalactiae (Group B streptococcus) has emerged as an important pathogen that affects humans and animals, including aquatic species. S. agalactiae infections are becoming an increasing problem in aquaculture and have been reported worldwide in a variety of fish species, especially those living in warm water. Recently, a very serious infectious disease of unknown aetiology broke out in ya‐fish (Schizothorax prenanti) farms in Sichuan Province. A Gram‐positive, chain‐forming coccus was isolated from moribund cultured ya‐fish. The goals of this study were to identify the bacterial strains isolated from diseased fish between 2009 and 2011 in Sichuan Province, China, to evaluate the pathogenicity of the pathogen in ya‐fish, crucian carp (Carassius carassius) and the Nile tilapia (Oreochromis niloticus); and to determine the susceptibility of the pathogen strains to many currently available anti‐microbial agents. The virulence tests were conducted by intraperitoneal injection of bacterial suspensions. In this study, four strains of a Gram‐positive, chain‐forming coccus were isolated from moribund cultured ya‐fish (S. prenanti). The coccoid microorganism was identified as S. agalactiae using a commercial streptococcal grouping kit and 16S rDNA sequencing analysis. Susceptibility of the isolates to 22 antibiotics was tested using the disc diffusion method. All isolates showed a similar antibiotic susceptibility, which were sensitive to amoxicillin, ciprofloxacin, lomefloxacin, chloramphenicol, rifampin, vancomycin, azithromycin, florfenicol, cefalexin, cefradine and deoxycycline and resistant to gentamicin, sinomin (SMZ/TMP), penicillin, tenemycin, fradiomycin and streptomycin. Furthermore, the virulence tests were conducted by intraperitoneal injection of the isolated strain GY101 in ya‐fish, crucian carp and the Nile tilapia. This coccus was lethal to ya‐fish, Nile tilapia and crucian carp. The mortality rates of infected ya‐fish were 100%, 100%, 60% and 20% at doses of 1.0 × 107, 1.0 × 106, 1.0 × 105 and 1.0 × 104 CFU/fish, respectively. The values were 100%, 80%, 60% and 30% for Nile tilapia and 100%, 60%, 10% and 0% for crucian carp. To our knowledge, this is the first report of S. agalactiae as a pathogen of ya‐fish.</abstract>
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