Molecular genetic analysis among subspecies of two Eurasian sturgeon species, Acipenser baerii and A. stellatus
Identifieur interne : 000E77 ( Istex/Corpus ); précédent : 000E76; suivant : 000E78Molecular genetic analysis among subspecies of two Eurasian sturgeon species, Acipenser baerii and A. stellatus
Auteurs : P. Doukakis ; V. J. Birstein ; G. I. Ruban ; R. DesalleSource :
- Molecular Ecology [ 0962-1083 ] ; 1999-12.
English descriptors
- KwdEn :
Abstract
Two species, the Siberian sturgeon, Acipenser baerii, and stellate sturgeon, A. stellatus, were studied using mitochondrial DNA (mtDNA) (D‐loop, cytochrome b (cyt‐b) and ND5/6 genes) sequencing to determine whether traditionally defined subspecies correspond to taxonomic entities and conservation management units. Initially, several mtDNA regions for each taxon (A. baerii: 737 bp D‐loop, 750 bp ND5, 200 bp ND6, and 790 bp cyt‐b;A. stellatus: 737 bp D‐loop and 600 bp ND5) were examined. The D‐loop was the most variable region and was sequenced for 35 A. baerii and 82 A. stellatus individuals. No fixed, diagnostic differences were found between any of the subspecies. Geographical structuring of haplotypes was observed within A. baerii, and gene flow estimates suggest isolation of the A. baerii baicalensis subspecies and the Yenisie and Lena River populations. No intraspecific subdivisioning was found within the genetic data for A. stellatus. The use of the phylogenetic criterion (fixed diagnostic differences) for identifying conservation units is compared to the rationale and results of other methods. Overall, morphologically and geographically based subspecies designations within Acipenseridae may not directly correspond to the biological entities appropriate for management and should not be used for conservation programmes without genetic support.
Url:
DOI: 10.1046/j.1365-294X.1999.00816.x
Links to Exploration step
ISTEX:315AA04C393DAB1B0600AE9CF62C1EA9E64A83B2Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Molecular genetic analysis among subspecies of two Eurasian sturgeon species, Acipenser baerii and A. stellatus</title><author><name sortKey="Doukakis, P" sort="Doukakis, P" uniqKey="Doukakis P" first="P." last="Doukakis">P. Doukakis</name><affiliation><mods:affiliation>American Museum of Natural History, Central Park West at 79th Street, New York, NY 10024, USA,</mods:affiliation></affiliation><affiliation><mods:affiliation>Department of Ecology/Evolutionary Biology, Graduate School of Arts and Science, Yale University, New Haven, CT 06511 USA,</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: doukakis@amnh.org</mods:affiliation></affiliation></author><author><name sortKey="Birstein, V J" sort="Birstein, V J" uniqKey="Birstein V" first="V. J." last="Birstein">V. J. Birstein</name><affiliation><mods:affiliation>American Museum of Natural History, Central Park West at 79th Street, New York, NY 10024, USA,</mods:affiliation></affiliation></author><author><name sortKey="Ruban, G I" sort="Ruban, G I" uniqKey="Ruban G" first="G. I." last="Ruban">G. I. Ruban</name><affiliation><mods:affiliation>Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences, Leninskii prospekt 33, Moscow, 117071 Russia</mods:affiliation></affiliation></author><author><name sortKey="Desalle, R" sort="Desalle, R" uniqKey="Desalle R" first="R." last="Desalle">R. Desalle</name><affiliation><mods:affiliation>American Museum of Natural History, Central Park West at 79th Street, New York, NY 10024, USA,</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:315AA04C393DAB1B0600AE9CF62C1EA9E64A83B2</idno><date when="1999" year="1999">1999</date><idno type="doi">10.1046/j.1365-294X.1999.00816.x</idno><idno type="url">https://api.istex.fr/document/315AA04C393DAB1B0600AE9CF62C1EA9E64A83B2/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000E77</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000E77</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Molecular genetic analysis among subspecies of two Eurasian sturgeon species, Acipenser baerii and A. stellatus</title><author><name sortKey="Doukakis, P" sort="Doukakis, P" uniqKey="Doukakis P" first="P." last="Doukakis">P. Doukakis</name><affiliation><mods:affiliation>American Museum of Natural History, Central Park West at 79th Street, New York, NY 10024, USA,</mods:affiliation></affiliation><affiliation><mods:affiliation>Department of Ecology/Evolutionary Biology, Graduate School of Arts and Science, Yale University, New Haven, CT 06511 USA,</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: doukakis@amnh.org</mods:affiliation></affiliation></author><author><name sortKey="Birstein, V J" sort="Birstein, V J" uniqKey="Birstein V" first="V. J." last="Birstein">V. J. Birstein</name><affiliation><mods:affiliation>American Museum of Natural History, Central Park West at 79th Street, New York, NY 10024, USA,</mods:affiliation></affiliation></author><author><name sortKey="Ruban, G I" sort="Ruban, G I" uniqKey="Ruban G" first="G. I." last="Ruban">G. I. Ruban</name><affiliation><mods:affiliation>Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences, Leninskii prospekt 33, Moscow, 117071 Russia</mods:affiliation></affiliation></author><author><name sortKey="Desalle, R" sort="Desalle, R" uniqKey="Desalle R" first="R." last="Desalle">R. Desalle</name><affiliation><mods:affiliation>American Museum of Natural History, Central Park West at 79th Street, New York, NY 10024, USA,</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Molecular Ecology</title><idno type="ISSN">0962-1083</idno><idno type="eISSN">1365-294X</idno><imprint><publisher>Blackwell Science Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="1999-12">1999-12</date><biblScope unit="volume">8</biblScope><biblScope unit="supplement">s1</biblScope><biblScope unit="page" from="S117">S117</biblScope><biblScope unit="page" to="S127">S127</biblScope></imprint><idno type="ISSN">0962-1083</idno></series><idno type="istex">315AA04C393DAB1B0600AE9CF62C1EA9E64A83B2</idno><idno type="DOI">10.1046/j.1365-294X.1999.00816.x</idno><idno type="ArticleID">MEC816</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0962-1083</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acipenser baerii</term><term>Acipenser stellatus</term><term>conservation</term><term>mtDNA</term><term>sturgeon</term><term>subspecies</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Two species, the Siberian sturgeon, Acipenser baerii, and stellate sturgeon, A. stellatus, were studied using mitochondrial DNA (mtDNA) (D‐loop, cytochrome b (cyt‐b) and ND5/6 genes) sequencing to determine whether traditionally defined subspecies correspond to taxonomic entities and conservation management units. Initially, several mtDNA regions for each taxon (A. baerii: 737 bp D‐loop, 750 bp ND5, 200 bp ND6, and 790 bp cyt‐b;A. stellatus: 737 bp D‐loop and 600 bp ND5) were examined. The D‐loop was the most variable region and was sequenced for 35 A. baerii and 82 A. stellatus individuals. No fixed, diagnostic differences were found between any of the subspecies. Geographical structuring of haplotypes was observed within A. baerii, and gene flow estimates suggest isolation of the A. baerii baicalensis subspecies and the Yenisie and Lena River populations. No intraspecific subdivisioning was found within the genetic data for A. stellatus. The use of the phylogenetic criterion (fixed diagnostic differences) for identifying conservation units is compared to the rationale and results of other methods. Overall, morphologically and geographically based subspecies designations within Acipenseridae may not directly correspond to the biological entities appropriate for management and should not be used for conservation programmes without genetic support.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>P. Doukakis</name><affiliations><json:string>American Museum of Natural History, Central Park West at 79th Street, New York, NY 10024, USA,</json:string><json:string>Department of Ecology/Evolutionary Biology, Graduate School of Arts and Science, Yale University, New Haven, CT 06511 USA,</json:string><json:string>E-mail: doukakis@amnh.org</json:string></affiliations></json:item><json:item><name>V. J. Birstein</name><affiliations><json:string>American Museum of Natural History, Central Park West at 79th Street, New York, NY 10024, USA,</json:string></affiliations></json:item><json:item><name>G. I. Ruban</name><affiliations><json:string>Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences, Leninskii prospekt 33, Moscow, 117071 Russia</json:string></affiliations></json:item><json:item><name>R. DeSalle</name><affiliations><json:string>American Museum of Natural History, Central Park West at 79th Street, New York, NY 10024, USA,</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Acipenser baerii</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Acipenser stellatus</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>conservation</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>mtDNA</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>sturgeon</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>subspecies</value></json:item></subject><articleId><json:string>MEC816</json:string></articleId><language><json:string>eng</json:string></language><originalGenre><json:string>article</json:string></originalGenre><abstract>Two species, the Siberian sturgeon, Acipenser baerii, and stellate sturgeon, A. stellatus, were studied using mitochondrial DNA (mtDNA) (D‐loop, cytochrome b (cyt‐b) and ND5/6 genes) sequencing to determine whether traditionally defined subspecies correspond to taxonomic entities and conservation management units. Initially, several mtDNA regions for each taxon (A. baerii: 737 bp D‐loop, 750 bp ND5, 200 bp ND6, and 790 bp cyt‐b;A. stellatus: 737 bp D‐loop and 600 bp ND5) were examined. The D‐loop was the most variable region and was sequenced for 35 A. baerii and 82 A. stellatus individuals. No fixed, diagnostic differences were found between any of the subspecies. Geographical structuring of haplotypes was observed within A. baerii, and gene flow estimates suggest isolation of the A. baerii baicalensis subspecies and the Yenisie and Lena River populations. No intraspecific subdivisioning was found within the genetic data for A. stellatus. The use of the phylogenetic criterion (fixed diagnostic differences) for identifying conservation units is compared to the rationale and results of other methods. Overall, morphologically and geographically based subspecies designations within Acipenseridae may not directly correspond to the biological entities appropriate for management and should not be used for conservation programmes without genetic support.</abstract><qualityIndicators><score>7.28</score><pdfVersion>1.2</pdfVersion><pdfPageSize>595 x 783 pts</pdfPageSize><refBibsNative>true</refBibsNative><abstractCharCount>1369</abstractCharCount><pdfWordCount>6380</pdfWordCount><pdfCharCount>41803</pdfCharCount><pdfPageCount>12</pdfPageCount><abstractWordCount>190</abstractWordCount></qualityIndicators><title>Molecular genetic analysis among subspecies of two Eurasian sturgeon species, Acipenser baerii and A. stellatus</title><genre><json:string>article</json:string></genre><host><volume>8</volume><publisherId><json:string>MEC</json:string></publisherId><pages><total>11</total><last>S127</last><first>S117</first></pages><issn><json:string>0962-1083</json:string></issn><genre><json:string>journal</json:string></genre><language><json:string>unknown</json:string></language><eissn><json:string>1365-294X</json:string></eissn><title>Molecular Ecology</title><doi><json:string>10.1111/(ISSN)1365-294X</json:string></doi></host><categories><wos><json:string>science</json:string><json:string>evolutionary biology</json:string><json:string>ecology</json:string><json:string>biochemistry & molecular biology</json:string></wos><scienceMetrix><json:string>natural sciences</json:string><json:string>biology</json:string><json:string>evolutionary biology</json:string></scienceMetrix></categories><publicationDate>1999</publicationDate><copyrightDate>1999</copyrightDate><doi><json:string>10.1046/j.1365-294X.1999.00816.x</json:string></doi><id>315AA04C393DAB1B0600AE9CF62C1EA9E64A83B2</id><score>0.39510968</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/document/315AA04C393DAB1B0600AE9CF62C1EA9E64A83B2/fulltext/pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/document/315AA04C393DAB1B0600AE9CF62C1EA9E64A83B2/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/315AA04C393DAB1B0600AE9CF62C1EA9E64A83B2/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Molecular genetic analysis among subspecies of two Eurasian sturgeon species, Acipenser baerii and A. stellatus</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>Blackwell Science Ltd</publisher><pubPlace>Oxford, UK</pubPlace><availability><p>WILEY</p></availability><date>1999</date></publicationStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Molecular genetic analysis among subspecies of two Eurasian sturgeon species, Acipenser baerii and A. stellatus</title><author xml:id="author-1"><persName><forename type="first">P.</forename><surname>Doukakis</surname></persName><email>doukakis@amnh.org</email><affiliation>American Museum of Natural History, Central Park West at 79th Street, New York, NY 10024, USA,</affiliation><affiliation>Department of Ecology/Evolutionary Biology, Graduate School of Arts and Science, Yale University, New Haven, CT 06511 USA,</affiliation></author><author xml:id="author-2"><persName><forename type="first">V. J.</forename><surname>Birstein</surname></persName><affiliation>American Museum of Natural History, Central Park West at 79th Street, New York, NY 10024, USA,</affiliation></author><author xml:id="author-3"><persName><forename type="first">G. I.</forename><surname>Ruban</surname></persName><affiliation>Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences, Leninskii prospekt 33, Moscow, 117071 Russia</affiliation></author><author xml:id="author-4"><persName><forename type="first">R.</forename><surname>DeSalle</surname></persName><affiliation>American Museum of Natural History, Central Park West at 79th Street, New York, NY 10024, USA,</affiliation></author></analytic><monogr><title level="j">Molecular Ecology</title><idno type="pISSN">0962-1083</idno><idno type="eISSN">1365-294X</idno><idno type="DOI">10.1111/(ISSN)1365-294X</idno><imprint><publisher>Blackwell Science Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="1999-12"></date><biblScope unit="volume">8</biblScope><biblScope unit="supplement">s1</biblScope><biblScope unit="page" from="S117">S117</biblScope><biblScope unit="page" to="S127">S127</biblScope></imprint></monogr><idno type="istex">315AA04C393DAB1B0600AE9CF62C1EA9E64A83B2</idno><idno type="DOI">10.1046/j.1365-294X.1999.00816.x</idno><idno type="ArticleID">MEC816</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1999</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Two species, the Siberian sturgeon, Acipenser baerii, and stellate sturgeon, A. stellatus, were studied using mitochondrial DNA (mtDNA) (D‐loop, cytochrome b (cyt‐b) and ND5/6 genes) sequencing to determine whether traditionally defined subspecies correspond to taxonomic entities and conservation management units. Initially, several mtDNA regions for each taxon (A. baerii: 737 bp D‐loop, 750 bp ND5, 200 bp ND6, and 790 bp cyt‐b;A. stellatus: 737 bp D‐loop and 600 bp ND5) were examined. The D‐loop was the most variable region and was sequenced for 35 A. baerii and 82 A. stellatus individuals. No fixed, diagnostic differences were found between any of the subspecies. Geographical structuring of haplotypes was observed within A. baerii, and gene flow estimates suggest isolation of the A. baerii baicalensis subspecies and the Yenisie and Lena River populations. No intraspecific subdivisioning was found within the genetic data for A. stellatus. The use of the phylogenetic criterion (fixed diagnostic differences) for identifying conservation units is compared to the rationale and results of other methods. Overall, morphologically and geographically based subspecies designations within Acipenseridae may not directly correspond to the biological entities appropriate for management and should not be used for conservation programmes without genetic support.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>keywords</head><item><term>Acipenser baerii</term></item><item><term>Acipenser stellatus</term></item><item><term>conservation</term></item><item><term>mtDNA</term></item><item><term>sturgeon</term></item><item><term>subspecies</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1999-12">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/315AA04C393DAB1B0600AE9CF62C1EA9E64A83B2/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>Blackwell Science Ltd</publisherName><publisherLoc>Oxford, UK</publisherLoc></publisherInfo><doi origin="wiley" registered="yes">10.1111/(ISSN)1365-294X</doi><issn type="print">0962-1083</issn><issn type="electronic">1365-294X</issn><idGroup><id type="product" value="MEC"></id><id type="publisherDivision" value="ST"></id></idGroup><titleGroup><title type="main" sort="MOLECULAR ECOLOGY">Molecular Ecology</title></titleGroup></publicationMeta><publicationMeta level="part" position="12000"><doi origin="wiley">10.1111/mec.1999.8.issue-s1</doi><numberingGroup><numbering type="journalVolume" number="8">8</numbering><numbering type="supplement">s1</numbering></numberingGroup><coverDate startDate="1999-12">December 1999</coverDate></publicationMeta><publicationMeta level="unit" type="article" position="0011700" status="forIssue"><doi origin="wiley">10.1046/j.1365-294X.1999.00816.x</doi><idGroup><id type="unit" value="MEC816"></id></idGroup><countGroup><count type="pageTotal" number="11"></count></countGroup><titleGroup><title type="tocHeading1">Original Articles</title></titleGroup><eventGroup><event type="firstOnline" date="2002-01-04"></event><event type="publishedOnlineFinalForm" date="2002-01-04"></event><event type="xmlConverted" agent="Converter:BPG_TO_WML3G version:2.3.2 mode:FullText source:Header result:Header" date="2010-03-10"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:4.0.1" date="2014-03-20"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-10-31"></event></eventGroup><numberingGroup><numbering type="pageFirst">S117</numbering><numbering type="pageLast">S127</numbering></numberingGroup><correspondenceTo> P. Doukakis. American Museum of Natural History, Central Park West at 79th Street, New York, NY 10024, USA. Fax: +1‐212‐769‐5277; E‐mail: <email>doukakis@amnh.org</email></correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:MEC.MEC816.pdf"></link></linkGroup></publicationMeta><contentMeta><unparsedEditorialHistory>Received 11 February 1999; revision received 19 May 1999;accepted 10 August 1999</unparsedEditorialHistory><countGroup><count type="figureTotal" number="4"></count><count type="tableTotal" number="2"></count><count type="formulaTotal" number="0"></count><count type="referenceTotal" number="71"></count><count type="wordTotal" number="6938"></count><count type="linksPubMed" number="0"></count><count type="linksCrossRef" number="0"></count></countGroup><titleGroup><title type="main">Molecular genetic analysis among subspecies of two Eurasian sturgeon species, <i>Acipenser baerii</i> and <i>A. stellatus</i></title><title type="shortAuthors"><sc>p. doukakis <i>et al</i>. </sc></title><title type="short"><sc>molecular analysis of sturgeon subspecies</sc></title></titleGroup><creators><creator creatorRole="author" xml:id="cr1" affiliationRef="#a1 #a2" corresponding="yes"><personName><givenNames>P.</givenNames><familyName>Doukakis</familyName></personName></creator><creator creatorRole="author" xml:id="cr2" affiliationRef="#a1"><personName><givenNames>V. J.</givenNames><familyName>Birstein</familyName></personName></creator><creator creatorRole="author" xml:id="cr3" affiliationRef="#a3"><personName><givenNames>G. I.</givenNames><familyName>Ruban</familyName></personName></creator><creator creatorRole="author" xml:id="cr4" affiliationRef="#a1"><personName><givenNames>R.</givenNames><familyName>DeSalle</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="a1" countryCode="US"><unparsedAffiliation>American Museum of Natural History, Central Park West at 79th Street, New York, NY 10024, USA,</unparsedAffiliation></affiliation><affiliation xml:id="a2"><unparsedAffiliation>Department of Ecology/Evolutionary Biology, Graduate School of Arts and Science, Yale University, New Haven, CT 06511 USA,</unparsedAffiliation></affiliation><affiliation xml:id="a3" countryCode="RU"><unparsedAffiliation>Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences, Leninskii prospekt 33, Moscow, 117071 Russia</unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en"><keyword xml:id="k1"><i>Acipenser baerii</i></keyword><keyword xml:id="k2"><i>Acipenser stellatus</i></keyword><keyword xml:id="k3">conservation</keyword><keyword xml:id="k4">mtDNA</keyword><keyword xml:id="k5">sturgeon</keyword><keyword xml:id="k6">subspecies</keyword></keywordGroup><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Abstract</title><p>Two species, the Siberian sturgeon, <i>Acipenser baerii</i>, and stellate sturgeon, <i>A. stellatus</i>, were studied using mitochondrial DNA (mtDNA) (D‐loop, cytochrome <i>b</i> (cyt‐<i>b</i>) and ND5/6 genes) sequencing to determine whether traditionally defined subspecies correspond to taxonomic entities and conservation management units. Initially, several mtDNA regions for each taxon (<i>A. baerii</i>: 737 bp D‐loop, 750 bp ND5, 200 bp ND6, and 790 bp cyt‐<i>b;</i><i>A. stellatus</i>: 737 bp D‐loop and 600 bp ND5) were examined. The D‐loop was the most variable region and was sequenced for 35 <i>A. baerii</i> and 82 <i>A. stellatus</i> individuals. No fixed, diagnostic differences were found between any of the subspecies. Geographical structuring of haplotypes was observed within <i>A. baerii</i>, and gene flow estimates suggest isolation of the <i>A. baerii baicalensis</i> subspecies and the Yenisie and Lena River populations. No intraspecific subdivisioning was found within the genetic data for <i>A. stellatus</i>. The use of the phylogenetic criterion (fixed diagnostic differences) for identifying conservation units is compared to the rationale and results of other methods. Overall, morphologically and geographically based subspecies designations within Acipenseridae may not directly correspond to the biological entities appropriate for management and should not be used for conservation programmes without genetic support.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Molecular genetic analysis among subspecies of two Eurasian sturgeon species, Acipenser baerii and A. stellatus</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>molecular analysis of sturgeon subspecies</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Molecular genetic analysis among subspecies of two Eurasian sturgeon species, Acipenser baerii and A. stellatus</title></titleInfo><name type="personal"><namePart type="given">P.</namePart><namePart type="family">Doukakis</namePart><affiliation>American Museum of Natural History, Central Park West at 79th Street, New York, NY 10024, USA,</affiliation><affiliation>Department of Ecology/Evolutionary Biology, Graduate School of Arts and Science, Yale University, New Haven, CT 06511 USA,</affiliation><affiliation>E-mail: doukakis@amnh.org</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">V. J.</namePart><namePart type="family">Birstein</namePart><affiliation>American Museum of Natural History, Central Park West at 79th Street, New York, NY 10024, USA,</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">G. I.</namePart><namePart type="family">Ruban</namePart><affiliation>Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences, Leninskii prospekt 33, Moscow, 117071 Russia</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">R.</namePart><namePart type="family">DeSalle</namePart><affiliation>American Museum of Natural History, Central Park West at 79th Street, New York, NY 10024, USA,</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Blackwell Science Ltd</publisher><place><placeTerm type="text">Oxford, UK</placeTerm></place><dateIssued encoding="w3cdtf">1999-12</dateIssued><edition>Received 11 February 1999; revision received 19 May 1999;accepted 10 August 1999</edition><copyrightDate encoding="w3cdtf">1999</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">4</extent><extent unit="tables">2</extent><extent unit="references">71</extent><extent unit="words">6938</extent></physicalDescription><abstract lang="en">Two species, the Siberian sturgeon, Acipenser baerii, and stellate sturgeon, A. stellatus, were studied using mitochondrial DNA (mtDNA) (D‐loop, cytochrome b (cyt‐b) and ND5/6 genes) sequencing to determine whether traditionally defined subspecies correspond to taxonomic entities and conservation management units. Initially, several mtDNA regions for each taxon (A. baerii: 737 bp D‐loop, 750 bp ND5, 200 bp ND6, and 790 bp cyt‐b;A. stellatus: 737 bp D‐loop and 600 bp ND5) were examined. The D‐loop was the most variable region and was sequenced for 35 A. baerii and 82 A. stellatus individuals. No fixed, diagnostic differences were found between any of the subspecies. Geographical structuring of haplotypes was observed within A. baerii, and gene flow estimates suggest isolation of the A. baerii baicalensis subspecies and the Yenisie and Lena River populations. No intraspecific subdivisioning was found within the genetic data for A. stellatus. The use of the phylogenetic criterion (fixed diagnostic differences) for identifying conservation units is compared to the rationale and results of other methods. Overall, morphologically and geographically based subspecies designations within Acipenseridae may not directly correspond to the biological entities appropriate for management and should not be used for conservation programmes without genetic support.</abstract><subject lang="en"><genre>keywords</genre><topic>Acipenser baerii</topic><topic>Acipenser stellatus</topic><topic>conservation</topic><topic>mtDNA</topic><topic>sturgeon</topic><topic>subspecies</topic></subject><relatedItem type="host"><titleInfo><title>Molecular Ecology</title></titleInfo><genre type="journal">journal</genre><identifier type="ISSN">0962-1083</identifier><identifier type="eISSN">1365-294X</identifier><identifier type="DOI">10.1111/(ISSN)1365-294X</identifier><identifier type="PublisherID">MEC</identifier><part><date>1999</date><detail type="volume"><caption>vol.</caption><number>8</number></detail><detail type="supplement"><caption>Suppl. no.</caption><number>s1</number></detail><extent unit="pages"><start>S117</start><end>S127</end><total>11</total></extent></part></relatedItem><identifier type="istex">315AA04C393DAB1B0600AE9CF62C1EA9E64A83B2</identifier><identifier type="DOI">10.1046/j.1365-294X.1999.00816.x</identifier><identifier type="ArticleID">MEC816</identifier><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>Blackwell Science Ltd</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Eau/explor/EsturgeonV1/Data/Istex/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000E77 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Corpus/biblio.hfd -nk 000E77 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Eau
|area= EsturgeonV1
|flux= Istex
|étape= Corpus
|type= RBID
|clé= ISTEX:315AA04C393DAB1B0600AE9CF62C1EA9E64A83B2
|texte= Molecular genetic analysis among subspecies of two Eurasian sturgeon species, Acipenser baerii and A. stellatus
}}
| This area was generated with Dilib version V0.6.27. |  |