A Novel In Vitro Model of Canine Malignant Hemangioendothelioma
Identifieur interne : 000E43 ( Istex/Corpus ); précédent : 000E42; suivant : 000E44A Novel In Vitro Model of Canine Malignant Hemangioendothelioma
Auteurs : D. Kühn ; B. Kohn ; L. Brunnberg ; J. PlendlSource :
- Anatomia, Histologia, Embryologia [ 0340-2096 ] ; 2005-12.
Abstract
Introduction and Aim: Canine malignant haemangioendothelioma is an aggressive neoplasia that affects mostly older dogs of large breeds with a strong predilection for the spleen, liver, heart and skin. The tumour originates in the vascular endothelium and consists of transformed cells forming large and leaky vessel‐like structures. Prognosis is poor because surgery and chemotherapy have limited success in prolonging survival times and increasing quality of patients. A new strategy to treat this malignancy could be anti‐angiogenic therapy based on the inhibition of proliferation, migration and three‐dimensional organization of transformed cells. In order to reduce animal experiments, in vitro‐models are required to test the safety and efficacy of anti‐angiogenic drugs. So far only few models of angiogenesis are available using mostly human, rodent and bovine cells. Therefore, the aim of our study was to establish an in vitro model of canine haemangioendothelioma.
Url:
DOI: 10.1111/j.1439-0264.2005.00669_64.x
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Kühn</name><affiliation><mods:affiliation>Institute of Veterinary Anatomy</mods:affiliation></affiliation></author><author><name sortKey="Kohn, B" sort="Kohn, B" uniqKey="Kohn B" first="B." last="Kohn">B. Kohn</name><affiliation><mods:affiliation>Clinic and Polyclinic for Small Animals, Free University of Berlin, D‐14163 Berlin, Germany</mods:affiliation></affiliation></author><author><name sortKey="Brunnberg, L" sort="Brunnberg, L" uniqKey="Brunnberg L" first="L." last="Brunnberg">L. Brunnberg</name><affiliation><mods:affiliation>Clinic and Polyclinic for Small Animals, Free University of Berlin, D‐14163 Berlin, Germany</mods:affiliation></affiliation></author><author><name sortKey="Plendl, J" sort="Plendl, J" uniqKey="Plendl J" first="J." last="Plendl">J. 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The tumour originates in the vascular endothelium and consists of transformed cells forming large and leaky vessel‐like structures. Prognosis is poor because surgery and chemotherapy have limited success in prolonging survival times and increasing quality of patients. A new strategy to treat this malignancy could be anti‐angiogenic therapy based on the inhibition of proliferation, migration and three‐dimensional organization of transformed cells. In order to reduce animal experiments, in vitro‐models are required to test the safety and efficacy of anti‐angiogenic drugs. So far only few models of angiogenesis are available using mostly human, rodent and bovine cells. Therefore, the aim of our study was to establish an in vitro model of canine haemangioendothelioma.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>D. Kühn</name><affiliations><json:string>Institute of Veterinary Anatomy</json:string></affiliations></json:item><json:item><name>B. 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The tumour originates in the vascular endothelium and consists of transformed cells forming large and leaky vessel‐like structures. Prognosis is poor because surgery and chemotherapy have limited success in prolonging survival times and increasing quality of patients. A new strategy to treat this malignancy could be anti‐angiogenic therapy based on the inhibition of proliferation, migration and three‐dimensional organization of transformed cells. In order to reduce animal experiments, in vitro‐models are required to test the safety and efficacy of anti‐angiogenic drugs. So far only few models of angiogenesis are available using mostly human, rodent and bovine cells. Therefore, the aim of our study was to establish an in vitro model of canine haemangioendothelioma.</p></abstract><abstract><p>Materials and Methods: Tumours were collected from dogs during surgery or immediately after euthanasia. Isolation of cells was done from different areas of the tumours and by enzymatic digestion of the tissue. Cells were incubated in culture media with and without endothelial growth factors. Cells were characterized by lectin histochemistry using Dolichos biflorus agglutinin, Ulex europaeus agglutinin and Bandeiraea simplicifolia agglutinin I. Moreover, RT‐PCR (polymerase chain reaction) was employed to investigate the expression of vascular endothelial growth factor (VEGF) and its endothelium‐specific receptors VEGF‐R1 and ‐R2.</p></abstract><abstract><p>Results and Conclusions: Different populations of cells were isolated and cultured successfully from canine malignant haemangioendothelioma. Cells show characteristics of microvascular endothelial cells of an angiogenic phenotype, i.e. the formation of spheroids and tube‐like structures as well as strong labelling for Bandeiraea simplicifolia agglutinin I. Thus, morphological and glycohistochemical results confirm the vascular character of the cells isolated. RT‐PCR showed expression of VEGF. However, endothelium‐specific VEGF receptors were not expressed. 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The tumour originates in the vascular endothelium and consists of transformed cells forming large and leaky vessel‐like structures. Prognosis is poor because surgery and chemotherapy have limited success in prolonging survival times and increasing quality of patients. A new strategy to treat this malignancy could be anti‐angiogenic therapy based on the inhibition of proliferation, migration and three‐dimensional organization of transformed cells. In order to reduce animal experiments, <i>in vitro</i>‐models are required to test the safety and efficacy of anti‐angiogenic drugs. So far only few models of angiogenesis are available using mostly human, rodent and bovine cells. Therefore, the aim of our study was to establish an <i>in vitro</i> model of canine haemangioendothelioma.</p><p><b>Materials and Methods: </b> Tumours were collected from dogs during surgery or immediately after euthanasia. Isolation of cells was done from different areas of the tumours and by enzymatic digestion of the tissue. Cells were incubated in culture media with and without endothelial growth factors. Cells were characterized by lectin histochemistry using <i>Dolichos biflorus</i> agglutinin, <i>Ulex europaeus</i> agglutinin and <i>Bandeiraea simplicifolia</i> agglutinin I. Moreover, RT‐PCR (polymerase chain reaction) was employed to investigate the expression of vascular endothelial growth factor (VEGF) and its endothelium‐specific receptors VEGF‐R1 and ‐R2.</p><p><b>Results and Conclusions: </b> Different populations of cells were isolated and cultured successfully from canine malignant haemangioendothelioma. Cells show characteristics of microvascular endothelial cells of an angiogenic phenotype, i.e. the formation of spheroids and tube‐like structures as well as strong labelling for <i>Bandeiraea simplicifolia</i> agglutinin I. Thus, morphological and glycohistochemical results confirm the vascular character of the cells isolated. RT‐PCR showed expression of VEGF. However, endothelium‐specific VEGF receptors were not expressed. Loss of typical receptors is common in cancer and may correlate with increased tumour dedifferentiation.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>A Novel In Vitro Model of Canine Malignant Hemangioendothelioma</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>Abstracts</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>A Novel In Vitro Model of Canine Malignant Hemangioendothelioma</title></titleInfo><name type="personal"><namePart type="given">D.</namePart><namePart type="family">Kühn</namePart><affiliation>Institute of Veterinary Anatomy</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">B.</namePart><namePart type="family">Kohn</namePart><affiliation>Clinic and Polyclinic for Small Animals, Free University of Berlin, D‐14163 Berlin, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">L.</namePart><namePart type="family">Brunnberg</namePart><affiliation>Clinic and Polyclinic for Small Animals, Free University of Berlin, D‐14163 Berlin, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J.</namePart><namePart type="family">Plendl</namePart><affiliation>Institute of Veterinary Anatomy</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="abstract" displayLabel="abstract"></genre><originInfo><publisher>Blackwell Verlag GmbH</publisher><place><placeTerm type="text">Berlin, Germany</placeTerm></place><dateIssued encoding="w3cdtf">2005-12</dateIssued><copyrightDate encoding="w3cdtf">2005</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract>Introduction and Aim: Canine malignant haemangioendothelioma is an aggressive neoplasia that affects mostly older dogs of large breeds with a strong predilection for the spleen, liver, heart and skin. The tumour originates in the vascular endothelium and consists of transformed cells forming large and leaky vessel‐like structures. Prognosis is poor because surgery and chemotherapy have limited success in prolonging survival times and increasing quality of patients. A new strategy to treat this malignancy could be anti‐angiogenic therapy based on the inhibition of proliferation, migration and three‐dimensional organization of transformed cells. In order to reduce animal experiments, in vitro‐models are required to test the safety and efficacy of anti‐angiogenic drugs. So far only few models of angiogenesis are available using mostly human, rodent and bovine cells. Therefore, the aim of our study was to establish an in vitro model of canine haemangioendothelioma.</abstract><abstract>Materials and Methods: Tumours were collected from dogs during surgery or immediately after euthanasia. Isolation of cells was done from different areas of the tumours and by enzymatic digestion of the tissue. Cells were incubated in culture media with and without endothelial growth factors. Cells were characterized by lectin histochemistry using Dolichos biflorus agglutinin, Ulex europaeus agglutinin and Bandeiraea simplicifolia agglutinin I. Moreover, RT‐PCR (polymerase chain reaction) was employed to investigate the expression of vascular endothelial growth factor (VEGF) and its endothelium‐specific receptors VEGF‐R1 and ‐R2.</abstract><abstract>Results and Conclusions: Different populations of cells were isolated and cultured successfully from canine malignant haemangioendothelioma. Cells show characteristics of microvascular endothelial cells of an angiogenic phenotype, i.e. the formation of spheroids and tube‐like structures as well as strong labelling for Bandeiraea simplicifolia agglutinin I. Thus, morphological and glycohistochemical results confirm the vascular character of the cells isolated. RT‐PCR showed expression of VEGF. However, endothelium‐specific VEGF receptors were not expressed. 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