Molecular Characterization, Sequence Analysis, and Taxonomic Position of Newly Isolated Fish Iridoviruses
Identifieur interne : 000E40 ( Istex/Corpus ); précédent : 000E39; suivant : 000E41Molecular Characterization, Sequence Analysis, and Taxonomic Position of Newly Isolated Fish Iridoviruses
Auteurs : Jinghe Mao ; R. P Hedrick ; V. G ChincharSource :
- Virology [ 0042-6822 ] ; 1997.
Abstract
Within the past decade, iridoviruses have been identified as the causative agents of systemic disease in a variety of commercially and recreationally important fish. Here we examine nine iridoviruses from fish, reptiles, and amphibians and demonstrate that all isolates were more similar to frog virus 3, the type species of the genusRanavirus,than to lymphocystis disease virus, the type species of the genusLymphocystivirus.Comparison of viral protein synthesis profiles, restriction endonuclease digestion patterns, and the amino acid sequence of the major capsid protein indicated that iridoviruses isolated from the same geographic region were similar, if not identical, whereas viruses from different areas were distinct. Moreover, using primers complementary to the conserved major capsid protein, we found that both PCR and RT-PCR successfully amplified virus-specific nucleic acid from all nine isolates. These studies demonstrate that the piscine iridoviruses examined here were members of the genusRanavirus,and suggest that surveys of pathogenic “fish viruses” may need to include neighboring amphibian and reptilian populations. In addition, the results indicate that PCR readily identified vertebrate iridoviruses and suggest that PCR will be useful in the diagnosis of fish disease.
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DOI: 10.1006/viro.1996.8435
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A. MurphyC. M. FauquetD. H. L. BishopS. A. GhabrialA. W. JarvisG. P. MartelliM. A. MayoM. D. 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Here we examine nine iridoviruses from fish, reptiles, and amphibians and demonstrate that all isolates were more similar to frog virus 3, the type species of the genusRanavirus,than to lymphocystis disease virus, the type species of the genusLymphocystivirus.Comparison of viral protein synthesis profiles, restriction endonuclease digestion patterns, and the amino acid sequence of the major capsid protein indicated that iridoviruses isolated from the same geographic region were similar, if not identical, whereas viruses from different areas were distinct. Moreover, using primers complementary to the conserved major capsid protein, we found that both PCR and RT-PCR successfully amplified virus-specific nucleic acid from all nine isolates. These studies demonstrate that the piscine iridoviruses examined here were members of the genusRanavirus,and suggest that surveys of pathogenic “fish viruses” may need to include neighboring amphibian and reptilian populations. 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A. MurphyC. M. FauquetD. H. L. BishopS. A. GhabrialA. W. JarvisG. P. MartelliM. A. MayoM. D. Summers, Eds.</ce:note-para></ce:article-footnote><ce:dochead><ce:textfn>Regular Article</ce:textfn></ce:dochead><ce:title>Molecular Characterization, Sequence Analysis, and Taxonomic Position of Newly Isolated Fish Iridoviruses</ce:title><ce:author-group><ce:author><ce:given-name>Jinghe</ce:given-name><ce:surname>Mao</ce:surname><ce:cross-ref refid="VY968435A1"><ce:sup>a</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>R.P</ce:given-name><ce:surname>Hedrick</ce:surname><ce:cross-ref refid="VY968435A2"><ce:sup>b</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>V.G</ce:given-name><ce:surname>Chinchar</ce:surname><ce:cross-ref refid="VY968435A1"><ce:sup>a</ce:sup></ce:cross-ref><ce:cross-ref refid="VY968435FN1"><ce:sup>1</ce:sup></ce:cross-ref></ce:author><ce:affiliation id="VY968435A1"><ce:label>a</ce:label><ce:textfn>Department of Microbiology, University of Mississippi Medical Center, Jackson, Mississippi, 39216</ce:textfn></ce:affiliation><ce:affiliation id="VY968435A2"><ce:label>b</ce:label><ce:textfn>Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, California, 95616</ce:textfn></ce:affiliation><ce:footnote id="VY968435FN1"><ce:label>1</ce:label><ce:note-para>To whom correspondence and reprint requests should be addressed. Fax: (601) 984-1708; E-mail: vgregc@fiona.umsmed.edu.</ce:note-para></ce:footnote></ce:author-group><ce:date-received day="29" month="10" year="1996"></ce:date-received><ce:date-revised day="25" month="11" year="1996"></ce:date-revised><ce:date-accepted day="30" month="12" year="1996"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>Within the past decade, iridoviruses have been identified as the causative agents of systemic disease in a variety of commercially and recreationally important fish. Here we examine nine iridoviruses from fish, reptiles, and amphibians and demonstrate that all isolates were more similar to frog virus 3, the type species of the genus<ce:italic>Ranavirus,</ce:italic>than to lymphocystis disease virus, the type species of the genus<ce:italic>Lymphocystivirus.</ce:italic>Comparison of viral protein synthesis profiles, restriction endonuclease digestion patterns, and the amino acid sequence of the major capsid protein indicated that iridoviruses isolated from the same geographic region were similar, if not identical, whereas viruses from different areas were distinct. Moreover, using primers complementary to the conserved major capsid protein, we found that both PCR and RT-PCR successfully amplified virus-specific nucleic acid from all nine isolates. These studies demonstrate that the piscine iridoviruses examined here were members of the genus<ce:italic>Ranavirus,</ce:italic>and suggest that surveys of pathogenic “fish viruses” may need to include neighboring amphibian and reptilian populations. In addition, the results indicate that PCR readily identified vertebrate iridoviruses and suggest that PCR will be useful in the diagnosis of fish disease.</ce:simple-para></ce:abstract-sec></ce:abstract></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Molecular Characterization, Sequence Analysis, and Taxonomic Position of Newly Isolated Fish Iridoviruses</title></titleInfo><titleInfo type="alternative" lang="en" contentType="CDATA"><title>Molecular Characterization, Sequence Analysis, and Taxonomic Position of Newly Isolated Fish Iridoviruses</title></titleInfo><name type="personal"><namePart type="given">Jinghe</namePart><namePart type="family">Mao</namePart><affiliation>Department of Microbiology, University of Mississippi Medical Center, Jackson, Mississippi, 39216</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">R.P</namePart><namePart type="family">Hedrick</namePart><affiliation>Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, California, 95616</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">V.G</namePart><namePart type="family">Chinchar</namePart><affiliation>Department of Microbiology, University of Mississippi Medical Center, Jackson, Mississippi, 39216</affiliation><description>To whom correspondence and reprint requests should be addressed. Fax: (601) 984-1708; E-mail: vgregc@fiona.umsmed.edu.</description><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article"></genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1997</dateIssued><dateModified encoding="w3cdtf">1996-11-25</dateModified><copyrightDate encoding="w3cdtf">1997</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">Within the past decade, iridoviruses have been identified as the causative agents of systemic disease in a variety of commercially and recreationally important fish. Here we examine nine iridoviruses from fish, reptiles, and amphibians and demonstrate that all isolates were more similar to frog virus 3, the type species of the genusRanavirus,than to lymphocystis disease virus, the type species of the genusLymphocystivirus.Comparison of viral protein synthesis profiles, restriction endonuclease digestion patterns, and the amino acid sequence of the major capsid protein indicated that iridoviruses isolated from the same geographic region were similar, if not identical, whereas viruses from different areas were distinct. Moreover, using primers complementary to the conserved major capsid protein, we found that both PCR and RT-PCR successfully amplified virus-specific nucleic acid from all nine isolates. These studies demonstrate that the piscine iridoviruses examined here were members of the genusRanavirus,and suggest that surveys of pathogenic “fish viruses” may need to include neighboring amphibian and reptilian populations. 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