Utility of a juvenile fathead minnow screening assay for detecting (anti‐)estrogenic substances
Identifieur interne : 000E03 ( Istex/Corpus ); précédent : 000E02; suivant : 000E04Utility of a juvenile fathead minnow screening assay for detecting (anti‐)estrogenic substances
Auteurs : Grace H. Panter ; Thomas H. Hutchinson ; Reinhard L Nge ; Christina M. Lye ; John P. Sumpter ; Melanie Zerulla ; Charles R. TylerSource :
- Environmental Toxicology and Chemistry [ 0730-7268 ] ; 2002-02.
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Abstract
The European Chemical Industry's aquatic research program for endocrine disrupters includes the development of an in vivo juvenile fathead minnow (Pimephales promelas) screening assay.Working within the Organization for Economic Cooperation and Development's (OECD, Paris, France) tiered approach to endocrine disrupter evaluation in fish, the juvenile fish screening protocol was adapted from the OECD test guideline 204. Six chemicals, with different (anti‐)estrogenic potencies, were used to develop the in vivo juvenile fish screening protocol: diethylstilbestrol, 17α‐ethynylestradiol, genistein, methoxychlor, 4‐tert‐pentylphenol, and ZM189,154 (a novel pharmaceutical antiestrogen). Mixed‐sex juvenile fathead minnows were exposed to individual chemicals (with chemical analyzes) and sampled after 4, 7, 14, and 21 d of exposure. Wet weight, total length, condition factor, and whole‐body homogenate concentrations of vitellogenin (VTG) were determined. Estrogens and antiestrogens were detected in this screen by virtue of the VTG response (an elevation or suppression, respectively) after 14 d. The study showed that the use of VTG concentrations in mixed‐sex juvenile fish provides a sensitive and robust assay for the detection of both estrogenic and antiestrogenic chemicals, with widely divergent potencies.
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DOI: 10.1002/etc.5620210213
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ISTEX:92D1C3D6D2926B50E3E7F114D92C67B7D9B11D43Le document en format XML
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Six chemicals, with different (anti‐)estrogenic potencies, were used to develop the in vivo juvenile fish screening protocol: diethylstilbestrol, 17α‐ethynylestradiol, genistein, methoxychlor, 4‐tert‐pentylphenol, and ZM189,154 (a novel pharmaceutical antiestrogen). Mixed‐sex juvenile fathead minnows were exposed to individual chemicals (with chemical analyzes) and sampled after 4, 7, 14, and 21 d of exposure. Wet weight, total length, condition factor, and whole‐body homogenate concentrations of vitellogenin (VTG) were determined. Estrogens and antiestrogens were detected in this screen by virtue of the VTG response (an elevation or suppression, respectively) after 14 d. 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Six chemicals, with different (anti‐)estrogenic potencies, were used to develop the in vivo juvenile fish screening protocol: diethylstilbestrol, 17α‐ethynylestradiol, genistein, methoxychlor, 4‐tert‐pentylphenol, and ZM189,154 (a novel pharmaceutical antiestrogen). Mixed‐sex juvenile fathead minnows were exposed to individual chemicals (with chemical analyzes) and sampled after 4, 7, 14, and 21 d of exposure. Wet weight, total length, condition factor, and whole‐body homogenate concentrations of vitellogenin (VTG) were determined. Estrogens and antiestrogens were detected in this screen by virtue of the VTG response (an elevation or suppression, respectively) after 14 d. 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affiliationRef="#af2"><personName><givenNames>Reinhard</givenNames><familyName>Länge</familyName></personName></creator><creator xml:id="au4" creatorRole="author" affiliationRef="#af3"><personName><givenNames>Christina M.</givenNames><familyName>Lye</familyName></personName></creator><creator xml:id="au5" creatorRole="author" affiliationRef="#af4"><personName><givenNames>John P.</givenNames><familyName>Sumpter</familyName></personName></creator><creator xml:id="au6" creatorRole="author" affiliationRef="#af2"><personName><givenNames>Melanie</givenNames><familyName>Zerulla</familyName></personName></creator><creator xml:id="au7" creatorRole="author" affiliationRef="#af5"><personName><givenNames>Charles R.</givenNames><familyName>Tyler</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="af1" countryCode="GB" type="organization"><unparsedAffiliation>AstraZeneca Global Safety, Health&Environment, Brixham Environmental Laboratory, Freshwater Quarry, Brixham, Devon TQ5 8BA, United Kingdom</unparsedAffiliation></affiliation><affiliation xml:id="af2" countryCode="DE" type="organization"><unparsedAffiliation>Experimental Toxicology, Schering AG, D‐13342, Berlin, Germany</unparsedAffiliation></affiliation><affiliation xml:id="af3" countryCode="GB" type="organization"><unparsedAffiliation>Shell Research&Technology Centre, Thornton, Cheshire CH1 3SH, United Kingdom</unparsedAffiliation></affiliation><affiliation xml:id="af4" countryCode="GB" type="organization"><unparsedAffiliation>Department of Biological Sciences, Brunel University, Uxbridge, Middlesex UB8 3PH, United Kingdom</unparsedAffiliation></affiliation><affiliation xml:id="af5" countryCode="GB" type="organization"><unparsedAffiliation>School of Biological Sciences, Hatherly Laboratories, Prince of Wales Road, Exeter University, Exeter, Devon EX4 4PS, United Kingdom</unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en" type="author"><keyword xml:id="kwd1">Screen</keyword><keyword xml:id="kwd2">Fathead minnow</keyword><keyword xml:id="kwd3">(Anti‐)estrogenic</keyword><keyword xml:id="kwd4">Vitellogenin</keyword></keywordGroup><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Abstract</title><p>The European Chemical Industry's aquatic research program for endocrine disrupters includes the development of an in vivo juvenile fathead minnow (<i>Pimephales promelas</i>) screening assay.Working within the Organization for Economic Cooperation and Development's (OECD, Paris, France) tiered approach to endocrine disrupter evaluation in fish, the juvenile fish screening protocol was adapted from the OECD test guideline 204. Six chemicals, with different (anti‐)estrogenic potencies, were used to develop the in vivo juvenile fish screening protocol: diethylstilbestrol, 17α‐ethynylestradiol, genistein, methoxychlor, 4‐<i>tert</i>‐pentylphenol, and ZM189,154 (a novel pharmaceutical antiestrogen). Mixed‐sex juvenile fathead minnows were exposed to individual chemicals (with chemical analyzes) and sampled after 4, 7, 14, and 21 d of exposure. Wet weight, total length, condition factor, and whole‐body homogenate concentrations of vitellogenin (VTG) were determined. Estrogens and antiestrogens were detected in this screen by virtue of the VTG response (an elevation or suppression, respectively) after 14 d. The study showed that the use of VTG concentrations in mixed‐sex juvenile fish provides a sensitive and robust assay for the detection of both estrogenic and antiestrogenic chemicals, with widely divergent potencies.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Utility of a juvenile fathead minnow screening assay for detecting (anti‐)estrogenic substances</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>Fish screening assay for (anti‐)estrogenic chemicals</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Utility of a juvenile fathead minnow screening assay for detecting (anti‐)estrogenic substances</title></titleInfo><name type="personal"><namePart type="given">Grace H.</namePart><namePart type="family">Panter</namePart><affiliation>AstraZeneca Global Safety, Health&Environment, Brixham Environmental Laboratory, Freshwater Quarry, Brixham, Devon TQ5 8BA, United Kingdom</affiliation><affiliation>AstraZeneca Global Safety, Health&Environment, Brixham Environmental Laboratory, Freshwater Quarry, Brixham, Devon TQ5 8BA, United Kingdom</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Thomas H.</namePart><namePart type="family">Hutchinson</namePart><affiliation>AstraZeneca Global Safety, Health&Environment, Brixham Environmental Laboratory, Freshwater Quarry, Brixham, Devon TQ5 8BA, United Kingdom</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Reinhard</namePart><namePart type="family">Länge</namePart><affiliation>Experimental Toxicology, Schering AG, D‐13342, Berlin, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Christina M.</namePart><namePart type="family">Lye</namePart><affiliation>Shell Research&Technology Centre, Thornton, Cheshire CH1 3SH, United Kingdom</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">John P.</namePart><namePart type="family">Sumpter</namePart><affiliation>Department of Biological Sciences, Brunel University, Uxbridge, Middlesex UB8 3PH, United Kingdom</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Melanie</namePart><namePart type="family">Zerulla</namePart><affiliation>Experimental Toxicology, Schering AG, D‐13342, Berlin, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Charles R.</namePart><namePart type="family">Tyler</namePart><affiliation>School of Biological Sciences, Hatherly Laboratories, Prince of Wales Road, Exeter University, Exeter, Devon EX4 4PS, United Kingdom</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Wiley Periodicals, Inc.</publisher><place><placeTerm type="text">Hoboken</placeTerm></place><dateIssued encoding="w3cdtf">2002-02</dateIssued><dateCaptured encoding="w3cdtf">2000-11-14</dateCaptured><dateValid encoding="w3cdtf">2001-06-25</dateValid><copyrightDate encoding="w3cdtf">2002</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">6</extent><extent unit="tables">3</extent><extent unit="references">45</extent></physicalDescription><abstract lang="en">The European Chemical Industry's aquatic research program for endocrine disrupters includes the development of an in vivo juvenile fathead minnow (Pimephales promelas) screening assay.Working within the Organization for Economic Cooperation and Development's (OECD, Paris, France) tiered approach to endocrine disrupter evaluation in fish, the juvenile fish screening protocol was adapted from the OECD test guideline 204. Six chemicals, with different (anti‐)estrogenic potencies, were used to develop the in vivo juvenile fish screening protocol: diethylstilbestrol, 17α‐ethynylestradiol, genistein, methoxychlor, 4‐tert‐pentylphenol, and ZM189,154 (a novel pharmaceutical antiestrogen). Mixed‐sex juvenile fathead minnows were exposed to individual chemicals (with chemical analyzes) and sampled after 4, 7, 14, and 21 d of exposure. Wet weight, total length, condition factor, and whole‐body homogenate concentrations of vitellogenin (VTG) were determined. Estrogens and antiestrogens were detected in this screen by virtue of the VTG response (an elevation or suppression, respectively) after 14 d. The study showed that the use of VTG concentrations in mixed‐sex juvenile fish provides a sensitive and robust assay for the detection of both estrogenic and antiestrogenic chemicals, with widely divergent potencies.</abstract><subject lang="en"><genre>keywords</genre><topic>Screen</topic><topic>Fathead minnow</topic><topic>(Anti‐)estrogenic</topic><topic>Vitellogenin</topic></subject><relatedItem type="host"><titleInfo><title>Environmental Toxicology and Chemistry</title><subTitle>An International Journal</subTitle></titleInfo><titleInfo type="abbreviated"><title>Environmental Toxicology and Chemistry</title></titleInfo><genre type="journal">journal</genre><subject><genre>article-category</genre><topic>Environmental Toxicology</topic></subject><identifier type="ISSN">0730-7268</identifier><identifier type="eISSN">1552-8618</identifier><identifier type="DOI">10.1002/(ISSN)1552-8618</identifier><identifier type="PublisherID">ETC</identifier><part><date>2002</date><detail type="volume"><caption>vol.</caption><number>21</number></detail><detail type="issue"><caption>no.</caption><number>2</number></detail><extent unit="pages"><start>319</start><end>326</end><total>8</total></extent></part></relatedItem><identifier type="istex">92D1C3D6D2926B50E3E7F114D92C67B7D9B11D43</identifier><identifier type="DOI">10.1002/etc.5620210213</identifier><identifier type="ArticleID">ETC5620210213</identifier><accessCondition type="use and reproduction" contentType="copyright">Copyright © 2002 SETAC</accessCondition><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>Wiley Periodicals, Inc.</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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