Effects of flavonoids on aromatase activity, an in vitro study
Identifieur interne : 000D96 ( Istex/Corpus ); précédent : 000D95; suivant : 000D97Effects of flavonoids on aromatase activity, an in vitro study
Auteurs : C. Pelissero ; M. J. P. Lenczowski ; D. Chinzi ; B. Davail-Cuisset ; J. P. Sumpter ; A. FostierSource :
- Journal of Steroid Biochemistry and Molecular Biology [ 0960-0760 ] ; 1996.
Abstract
In the study, the inhibitory effect of flavonoids, including isoflavonic phytoestrogens, on the ovarian aromatase enzyme complex from the rainbow trout, Oncorhynchus mykiss, was assessed in vitro. Some of the compounds tested on fish were also tested on human placental aromatase activity as a comparison between the two sources of enzyme. It was found that flavone, dl-aminoglutethimide, apigenin, quercetin, 7,4′-dihydroxyflavone, α-naphthoflavone and equol were potent inhibitors of the ovarian aromatase activity in rainbow trout. Relative potencies (RP) of these compounds compared to flavone (assigned an effect of 1) were, respectively, 19.0, 8.7, 5.3, 3.7, 3.2 and 0.9. Two other phytoestrogens, namely biochanin A and genistein, slightly inhibited aromatase activity. Finally, 7-hydroxyflavone, formononetin, daidzein, coumestrol, chrysin, flavanone and estradiol-17β did not inhibit ovarian aromatase activity at doses up to 1000 μM. Experiments on human placental aromatase showed inhibitory effects of dl-aminoglutethimide, flavone, flavanone and equol with RP values of 2.8, 1, 1.5 and 0.4, respectively. These results are in accordance with previous studies. The influence of the experimental procedure on IC50 values and RP is discussed.
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DOI: 10.1016/0960-0760(95)00261-8
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Chinzi</name><affiliation><mods:affiliation>ENITA Bordeaux, Ave Général de Gaulle, 33175 Gradignan Cedex, France</mods:affiliation></affiliation></author><author><name sortKey="Davail Cuisset, B" sort="Davail Cuisset, B" uniqKey="Davail Cuisset B" first="B." last="Davail-Cuisset">B. Davail-Cuisset</name><affiliation><mods:affiliation>Unité INRA de Biologie de la Reproduction des Poissons, Université Bordeaux I, Avenue des Facultés, 33405 Talence Cedex, France</mods:affiliation></affiliation></author><author><name sortKey="Sumpter, J P" sort="Sumpter, J P" uniqKey="Sumpter J" first="J. P." last="Sumpter">J. P. Sumpter</name><affiliation><mods:affiliation>Department of Biology and Biochemistry, Brunel University, Uxbridge, Middlesex UB8 3PH, U.K.</mods:affiliation></affiliation></author><author><name sortKey="Fostier, A" sort="Fostier, A" uniqKey="Fostier A" first="A." last="Fostier">A. 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Davail-Cuisset</name><affiliation><mods:affiliation>Unité INRA de Biologie de la Reproduction des Poissons, Université Bordeaux I, Avenue des Facultés, 33405 Talence Cedex, France</mods:affiliation></affiliation></author><author><name sortKey="Sumpter, J P" sort="Sumpter, J P" uniqKey="Sumpter J" first="J. P." last="Sumpter">J. P. Sumpter</name><affiliation><mods:affiliation>Department of Biology and Biochemistry, Brunel University, Uxbridge, Middlesex UB8 3PH, U.K.</mods:affiliation></affiliation></author><author><name sortKey="Fostier, A" sort="Fostier, A" uniqKey="Fostier A" first="A." last="Fostier">A. Fostier</name><affiliation><mods:affiliation>Laboratoire INRA de Physiologie des Poissons, Université de Rennes I, 35042 Rennes, France</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Steroid Biochemistry and Molecular Biology</title><title level="j" type="abbrev">SBMB</title><idno type="ISSN">0960-0760</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1996">1996</date><biblScope unit="volume">57</biblScope><biblScope unit="issue">3–4</biblScope><biblScope unit="page" from="215">215</biblScope><biblScope unit="page" to="223">223</biblScope></imprint><idno type="ISSN">0960-0760</idno></series><idno type="istex">83C88DFC377346CEEA4956B1475D3367C5B343A5</idno><idno type="DOI">10.1016/0960-0760(95)00261-8</idno><idno type="PII">0960-0760(95)00261-8</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0960-0760</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In the study, the inhibitory effect of flavonoids, including isoflavonic phytoestrogens, on the ovarian aromatase enzyme complex from the rainbow trout, Oncorhynchus mykiss, was assessed in vitro. Some of the compounds tested on fish were also tested on human placental aromatase activity as a comparison between the two sources of enzyme. It was found that flavone, dl-aminoglutethimide, apigenin, quercetin, 7,4′-dihydroxyflavone, α-naphthoflavone and equol were potent inhibitors of the ovarian aromatase activity in rainbow trout. Relative potencies (RP) of these compounds compared to flavone (assigned an effect of 1) were, respectively, 19.0, 8.7, 5.3, 3.7, 3.2 and 0.9. Two other phytoestrogens, namely biochanin A and genistein, slightly inhibited aromatase activity. Finally, 7-hydroxyflavone, formononetin, daidzein, coumestrol, chrysin, flavanone and estradiol-17β did not inhibit ovarian aromatase activity at doses up to 1000 μM. Experiments on human placental aromatase showed inhibitory effects of dl-aminoglutethimide, flavone, flavanone and equol with RP values of 2.8, 1, 1.5 and 0.4, respectively. These results are in accordance with previous studies. The influence of the experimental procedure on IC50 values and RP is discussed.</div></front></TEI><istex><corpusName>elsevier</corpusName><author><json:item><name>C. Pelissero</name><affiliations><json:string>ENITA Bordeaux, Ave Général de Gaulle, 33175 Gradignan Cedex, France</json:string></affiliations></json:item><json:item><name>M.J.P. Lenczowski</name><affiliations><json:string>Department of Experimental Zoology, Research Group Comparative Endocrinology, University of Utrecht, Padualaan 8, 3584 CH Utrecht, The Netherlands</json:string></affiliations></json:item><json:item><name>D. Chinzi</name><affiliations><json:string>ENITA Bordeaux, Ave Général de Gaulle, 33175 Gradignan Cedex, France</json:string></affiliations></json:item><json:item><name>B. 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Some of the compounds tested on fish were also tested on human placental aromatase activity as a comparison between the two sources of enzyme. It was found that flavone, dl-aminoglutethimide, apigenin, quercetin, 7,4′-dihydroxyflavone, α-naphthoflavone and equol were potent inhibitors of the ovarian aromatase activity in rainbow trout. Relative potencies (RP) of these compounds compared to flavone (assigned an effect of 1) were, respectively, 19.0, 8.7, 5.3, 3.7, 3.2 and 0.9. Two other phytoestrogens, namely biochanin A and genistein, slightly inhibited aromatase activity. Finally, 7-hydroxyflavone, formononetin, daidzein, coumestrol, chrysin, flavanone and estradiol-17β did not inhibit ovarian aromatase activity at doses up to 1000 μM. Experiments on human placental aromatase showed inhibitory effects of dl-aminoglutethimide, flavone, flavanone and equol with RP values of 2.8, 1, 1.5 and 0.4, respectively. These results are in accordance with previous studies. 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Pelissero.</p></note><affiliation>ENITA Bordeaux, Ave Général de Gaulle, 33175 Gradignan Cedex, France</affiliation></author><author xml:id="author-2"><persName><forename type="first">M.J.P.</forename><surname>Lenczowski</surname></persName><affiliation>Department of Experimental Zoology, Research Group Comparative Endocrinology, University of Utrecht, Padualaan 8, 3584 CH Utrecht, The Netherlands</affiliation></author><author xml:id="author-3"><persName><forename type="first">D.</forename><surname>Chinzi</surname></persName><affiliation>ENITA Bordeaux, Ave Général de Gaulle, 33175 Gradignan Cedex, France</affiliation></author><author xml:id="author-4"><persName><forename type="first">B.</forename><surname>Davail-Cuisset</surname></persName><affiliation>Unité INRA de Biologie de la Reproduction des Poissons, Université Bordeaux I, Avenue des Facultés, 33405 Talence Cedex, France</affiliation></author><author xml:id="author-5"><persName><forename type="first">J.P.</forename><surname>Sumpter</surname></persName><affiliation>Department of Biology and Biochemistry, Brunel University, Uxbridge, Middlesex UB8 3PH, U.K.</affiliation></author><author xml:id="author-6"><persName><forename type="first">A.</forename><surname>Fostier</surname></persName><affiliation>Laboratoire INRA de Physiologie des Poissons, Université de Rennes I, 35042 Rennes, France</affiliation></author></analytic><monogr><title level="j">Journal of Steroid Biochemistry and Molecular Biology</title><title level="j" type="abbrev">SBMB</title><idno type="pISSN">0960-0760</idno><idno type="PII">S0960-0760(00)X0002-7</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1996"></date><biblScope unit="volume">57</biblScope><biblScope unit="issue">3–4</biblScope><biblScope unit="page" from="215">215</biblScope><biblScope unit="page" to="223">223</biblScope></imprint></monogr><idno type="istex">83C88DFC377346CEEA4956B1475D3367C5B343A5</idno><idno type="DOI">10.1016/0960-0760(95)00261-8</idno><idno type="PII">0960-0760(95)00261-8</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1996</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>In the study, the inhibitory effect of flavonoids, including isoflavonic phytoestrogens, on the ovarian aromatase enzyme complex from the rainbow trout, Oncorhynchus mykiss, was assessed in vitro. Some of the compounds tested on fish were also tested on human placental aromatase activity as a comparison between the two sources of enzyme. It was found that flavone, dl-aminoglutethimide, apigenin, quercetin, 7,4′-dihydroxyflavone, α-naphthoflavone and equol were potent inhibitors of the ovarian aromatase activity in rainbow trout. Relative potencies (RP) of these compounds compared to flavone (assigned an effect of 1) were, respectively, 19.0, 8.7, 5.3, 3.7, 3.2 and 0.9. Two other phytoestrogens, namely biochanin A and genistein, slightly inhibited aromatase activity. Finally, 7-hydroxyflavone, formononetin, daidzein, coumestrol, chrysin, flavanone and estradiol-17β did not inhibit ovarian aromatase activity at doses up to 1000 μM. Experiments on human placental aromatase showed inhibitory effects of dl-aminoglutethimide, flavone, flavanone and equol with RP values of 2.8, 1, 1.5 and 0.4, respectively. These results are in accordance with previous studies. The influence of the experimental procedure on IC50 values and RP is discussed.</p></abstract></profileDesc><revisionDesc><change when="1996">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/83C88DFC377346CEEA4956B1475D3367C5B343A5/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>SBMB</jid><aid>95002618</aid><ce:pii>0960-0760(95)00261-8</ce:pii><ce:doi>10.1016/0960-0760(95)00261-8</ce:doi><ce:copyright type="unknown" year="1996"></ce:copyright></item-info><head><ce:title>Effects of flavonoids on aromatase activity, an <ce:italic>in vitro</ce:italic> study</ce:title><ce:author-group><ce:author><ce:given-name>C.</ce:given-name><ce:surname>Pelissero</ce:surname><ce:cross-ref refid="COR1"><ce:sup>★</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF1"><ce:sup>1</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF2"><ce:sup>2</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>M.J.P.</ce:given-name><ce:surname>Lenczowski</ce:surname><ce:cross-ref refid="AFF3"><ce:sup>3</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>D.</ce:given-name><ce:surname>Chinzi</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>1</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>B.</ce:given-name><ce:surname>Davail-Cuisset</ce:surname><ce:cross-ref refid="AFF2"><ce:sup>2</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>J.P.</ce:given-name><ce:surname>Sumpter</ce:surname><ce:cross-ref refid="AFF4"><ce:sup>4</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>A.</ce:given-name><ce:surname>Fostier</ce:surname><ce:cross-ref refid="AFF5"><ce:sup>5</ce:sup></ce:cross-ref></ce:author><ce:affiliation id="AFF1"><ce:label>a</ce:label><ce:textfn>ENITA Bordeaux, Ave Général de Gaulle, 33175 Gradignan Cedex, France</ce:textfn></ce:affiliation><ce:affiliation id="AFF2"><ce:label>b</ce:label><ce:textfn>Unité INRA de Biologie de la Reproduction des Poissons, Université Bordeaux I, Avenue des Facultés, 33405 Talence Cedex, France</ce:textfn></ce:affiliation><ce:affiliation id="AFF3"><ce:label>c</ce:label><ce:textfn>Department of Experimental Zoology, Research Group Comparative Endocrinology, University of Utrecht, Padualaan 8, 3584 CH Utrecht, The Netherlands</ce:textfn></ce:affiliation><ce:affiliation id="AFF4"><ce:label>d</ce:label><ce:textfn>Department of Biology and Biochemistry, Brunel University, Uxbridge, Middlesex UB8 3PH, U.K.</ce:textfn></ce:affiliation><ce:affiliation id="AFF5"><ce:label>e</ce:label><ce:textfn>Laboratoire INRA de Physiologie des Poissons, Université de Rennes I, 35042 Rennes, France</ce:textfn></ce:affiliation><ce:correspondence id="COR1"><ce:label>★</ce:label><ce:text>Correspondence to C. Pelissero.</ce:text></ce:correspondence></ce:author-group><ce:date-received day="9" month="3" year="1995"></ce:date-received><ce:date-accepted day="11" month="10" year="1995"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>In the study, the inhibitory effect of flavonoids, including isoflavonic phytoestrogens, on the ovarian aromatase enzyme complex from the rainbow trout, <ce:italic>Oncorhynchus mykiss</ce:italic>, was assessed <ce:italic>in vitro</ce:italic>. Some of the compounds tested on fish were also tested on human placental aromatase activity as a comparison between the two sources of enzyme. It was found that flavone, <ce:italic>dl</ce:italic>-aminoglutethimide, apigenin, quercetin, 7,4′-dihydroxyflavone, α-naphthoflavone and equol were potent inhibitors of the ovarian aromatase activity in rainbow trout. Relative potencies (RP) of these compounds compared to flavone (assigned an effect of 1) were, respectively, 19.0, 8.7, 5.3, 3.7, 3.2 and 0.9. Two other phytoestrogens, namely biochanin A and genistein, slightly inhibited aromatase activity. Finally, 7-hydroxyflavone, formononetin, daidzein, coumestrol, chrysin, flavanone and estradiol-17β did not inhibit ovarian aromatase activity at doses up to 1000 μM. Experiments on human placental aromatase showed inhibitory effects of <ce:italic>dl</ce:italic>-aminoglutethimide, flavone, flavanone and equol with RP values of 2.8, 1, 1.5 and 0.4, respectively. These results are in accordance with previous studies. The influence of the experimental procedure on IC<ce:inf>50</ce:inf> values and RP is discussed.</ce:simple-para></ce:abstract-sec></ce:abstract></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Effects of flavonoids on aromatase activity, an in vitro study</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Effects of flavonoids on aromatase activity, an</title></titleInfo><name type="personal"><namePart type="given">C.</namePart><namePart type="family">Pelissero</namePart><affiliation>ENITA Bordeaux, Ave Général de Gaulle, 33175 Gradignan Cedex, France</affiliation><description>Correspondence to C. Pelissero.</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.J.P.</namePart><namePart type="family">Lenczowski</namePart><affiliation>Department of Experimental Zoology, Research Group Comparative Endocrinology, University of Utrecht, Padualaan 8, 3584 CH Utrecht, The Netherlands</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">D.</namePart><namePart type="family">Chinzi</namePart><affiliation>ENITA Bordeaux, Ave Général de Gaulle, 33175 Gradignan Cedex, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">B.</namePart><namePart type="family">Davail-Cuisset</namePart><affiliation>Unité INRA de Biologie de la Reproduction des Poissons, Université Bordeaux I, Avenue des Facultés, 33405 Talence Cedex, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J.P.</namePart><namePart type="family">Sumpter</namePart><affiliation>Department of Biology and Biochemistry, Brunel University, Uxbridge, Middlesex UB8 3PH, U.K.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">A.</namePart><namePart type="family">Fostier</namePart><affiliation>Laboratoire INRA de Physiologie des Poissons, Université de Rennes I, 35042 Rennes, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article"></genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1996</dateIssued><copyrightDate encoding="w3cdtf">1996</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">In the study, the inhibitory effect of flavonoids, including isoflavonic phytoestrogens, on the ovarian aromatase enzyme complex from the rainbow trout, Oncorhynchus mykiss, was assessed in vitro. Some of the compounds tested on fish were also tested on human placental aromatase activity as a comparison between the two sources of enzyme. It was found that flavone, dl-aminoglutethimide, apigenin, quercetin, 7,4′-dihydroxyflavone, α-naphthoflavone and equol were potent inhibitors of the ovarian aromatase activity in rainbow trout. Relative potencies (RP) of these compounds compared to flavone (assigned an effect of 1) were, respectively, 19.0, 8.7, 5.3, 3.7, 3.2 and 0.9. Two other phytoestrogens, namely biochanin A and genistein, slightly inhibited aromatase activity. Finally, 7-hydroxyflavone, formononetin, daidzein, coumestrol, chrysin, flavanone and estradiol-17β did not inhibit ovarian aromatase activity at doses up to 1000 μM. Experiments on human placental aromatase showed inhibitory effects of dl-aminoglutethimide, flavone, flavanone and equol with RP values of 2.8, 1, 1.5 and 0.4, respectively. These results are in accordance with previous studies. The influence of the experimental procedure on IC50 values and RP is discussed.</abstract><relatedItem type="host"><titleInfo><title>Journal of Steroid Biochemistry and Molecular Biology</title></titleInfo><titleInfo type="abbreviated"><title>SBMB</title></titleInfo><genre type="journal">journal</genre><originInfo><dateIssued encoding="w3cdtf">199602</dateIssued></originInfo><identifier type="ISSN">0960-0760</identifier><identifier type="PII">S0960-0760(00)X0002-7</identifier><part><date>199602</date><detail type="volume"><number>57</number><caption>vol.</caption></detail><detail type="issue"><number>3–4</number><caption>no.</caption></detail><extent unit="issue pages"><start>153</start><end>257</end></extent><extent unit="pages"><start>215</start><end>223</end></extent></part></relatedItem><identifier type="istex">83C88DFC377346CEEA4956B1475D3367C5B343A5</identifier><identifier type="DOI">10.1016/0960-0760(95)00261-8</identifier><identifier type="PII">0960-0760(95)00261-8</identifier><recordInfo><recordContentSource>ELSEVIER</recordContentSource></recordInfo></mods></metadata></istex></record>Pour manipuler ce document sous Unix (Dilib)
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