The use of pulse‐echo acoustic microscopy to non‐invasively determine the sex of living larval sea lampreys
Identifieur interne : 000D88 ( Istex/Corpus ); précédent : 000D87; suivant : 000D89The use of pulse‐echo acoustic microscopy to non‐invasively determine the sex of living larval sea lampreys
Auteurs : E. Maeva ; I. Bruno ; B. S. Zielinski ; M. F. Docker ; F. M. Severin ; R. G. MaevSource :
- Journal of Fish Biology [ 0022-1112 ] ; 2004-07.
English descriptors
Abstract
A rapid, accurate and non‐invasive method to determine the sex of larval sea lampreys Petromyzon marinus, using wide‐field pulse‐scanning acoustic microscopy, is described. Cross‐sectional pulse‐echo scans were made with a high‐resolution acoustic microscope in 48 larvae (110–130 mm total length, LT), and the acoustic images generated showed such internal body structures as the gonad, intestine, kidneys, cardinal veins, notochord and musculature. Females were identified by the presence of a relatively large (1–1·5 mm diameter) ovary, which was considerably less reflective to the 15 to 25 MHz acoustic signals than the surrounding kidney tissue. Males were recognized by the lack of the large non‐reflective ovary and, in some cases, the appearance of a small (0·2–0·3 mm) testis with slightly stronger reflective properties than the kidney. Identification of sex was confirmed by optical microscopy following dissection, and in a blind test on an additional 10 specimens (121–168 mm LT), the acoustic method reliably identified sex in 100% of the larvae. These results indicate that acoustic microscopy can determine the sex of live sea lamprey larvae in c. 30 s per animal, a process which until now required dissection or invasive surgery.
Url:
DOI: 10.1111/j.0022-1112.2004.00439.x
Links to Exploration step
ISTEX:9E3FE084B7E812AD4C8DE1A5716DAA34D3F225FELe document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">The use of pulse‐echo acoustic microscopy to non‐invasively determine the sex of living larval sea lampreys</title>
<author><name sortKey="Maeva, E" sort="Maeva, E" uniqKey="Maeva E" first="E." last="Maeva">E. Maeva</name>
<affiliation><mods:affiliation>Department of Physics, University of Windsor, Windsor, Ontario, N9B 3P4, Canada,</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Bruno, I" sort="Bruno, I" uniqKey="Bruno I" first="I." last="Bruno">I. Bruno</name>
<affiliation><mods:affiliation>Department of Physics, University of Windsor, Windsor, Ontario, N9B 3P4, Canada,</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Zielinski, B S" sort="Zielinski, B S" uniqKey="Zielinski B" first="B. S." last="Zielinski">B. S. Zielinski</name>
<affiliation><mods:affiliation>Department of Biological Sciences, University of Windsor, Windsor, Ontario, N9B 3P4, Canada and</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Docker, M F" sort="Docker, M F" uniqKey="Docker M" first="M. F." last="Docker">M. F. Docker</name>
<affiliation><mods:affiliation>Great Lakes Institute for Environmental Research, University of Windsor, Windsor, Ontario, N9B 3P4, Canada</mods:affiliation>
</affiliation>
<affiliation><mods:affiliation>E-mail: docker@uwindsor.ca</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Severin, F M" sort="Severin, F M" uniqKey="Severin F" first="F. M." last="Severin">F. M. Severin</name>
<affiliation><mods:affiliation>Department of Physics, University of Windsor, Windsor, Ontario, N9B 3P4, Canada,</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Maev, R G" sort="Maev, R G" uniqKey="Maev R" first="R. G." last="Maev">R. G. Maev</name>
<affiliation><mods:affiliation>Department of Physics, University of Windsor, Windsor, Ontario, N9B 3P4, Canada,</mods:affiliation>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:9E3FE084B7E812AD4C8DE1A5716DAA34D3F225FE</idno>
<date when="2004" year="2004">2004</date>
<idno type="doi">10.1111/j.0022-1112.2004.00439.x</idno>
<idno type="url">https://api.istex.fr/document/9E3FE084B7E812AD4C8DE1A5716DAA34D3F225FE/fulltext/pdf</idno>
<idno type="wicri:Area/Istex/Corpus">000D88</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000D88</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">The use of pulse‐echo acoustic microscopy to non‐invasively determine the sex of living larval sea lampreys</title>
<author><name sortKey="Maeva, E" sort="Maeva, E" uniqKey="Maeva E" first="E." last="Maeva">E. Maeva</name>
<affiliation><mods:affiliation>Department of Physics, University of Windsor, Windsor, Ontario, N9B 3P4, Canada,</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Bruno, I" sort="Bruno, I" uniqKey="Bruno I" first="I." last="Bruno">I. Bruno</name>
<affiliation><mods:affiliation>Department of Physics, University of Windsor, Windsor, Ontario, N9B 3P4, Canada,</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Zielinski, B S" sort="Zielinski, B S" uniqKey="Zielinski B" first="B. S." last="Zielinski">B. S. Zielinski</name>
<affiliation><mods:affiliation>Department of Biological Sciences, University of Windsor, Windsor, Ontario, N9B 3P4, Canada and</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Docker, M F" sort="Docker, M F" uniqKey="Docker M" first="M. F." last="Docker">M. F. Docker</name>
<affiliation><mods:affiliation>Great Lakes Institute for Environmental Research, University of Windsor, Windsor, Ontario, N9B 3P4, Canada</mods:affiliation>
</affiliation>
<affiliation><mods:affiliation>E-mail: docker@uwindsor.ca</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Severin, F M" sort="Severin, F M" uniqKey="Severin F" first="F. M." last="Severin">F. M. Severin</name>
<affiliation><mods:affiliation>Department of Physics, University of Windsor, Windsor, Ontario, N9B 3P4, Canada,</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Maev, R G" sort="Maev, R G" uniqKey="Maev R" first="R. G." last="Maev">R. G. Maev</name>
<affiliation><mods:affiliation>Department of Physics, University of Windsor, Windsor, Ontario, N9B 3P4, Canada,</mods:affiliation>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">Journal of Fish Biology</title>
<idno type="ISSN">0022-1112</idno>
<idno type="eISSN">1095-8649</idno>
<imprint><publisher>Blackwell Science Ltd</publisher>
<pubPlace>Oxford, UK; Malden, USA</pubPlace>
<date type="published" when="2004-07">2004-07</date>
<biblScope unit="volume">65</biblScope>
<biblScope unit="issue">1</biblScope>
<biblScope unit="page" from="148">148</biblScope>
<biblScope unit="page" to="156">156</biblScope>
</imprint>
<idno type="ISSN">0022-1112</idno>
</series>
<idno type="istex">9E3FE084B7E812AD4C8DE1A5716DAA34D3F225FE</idno>
<idno type="DOI">10.1111/j.0022-1112.2004.00439.x</idno>
<idno type="ArticleID">JFB439</idno>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0022-1112</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Petromyzon marinus</term>
<term>acoustic microscopy</term>
<term>sea lamprey</term>
<term>sex determination</term>
<term>ultrasound</term>
</keywords>
</textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">A rapid, accurate and non‐invasive method to determine the sex of larval sea lampreys Petromyzon marinus, using wide‐field pulse‐scanning acoustic microscopy, is described. Cross‐sectional pulse‐echo scans were made with a high‐resolution acoustic microscope in 48 larvae (110–130 mm total length, LT), and the acoustic images generated showed such internal body structures as the gonad, intestine, kidneys, cardinal veins, notochord and musculature. Females were identified by the presence of a relatively large (1–1·5 mm diameter) ovary, which was considerably less reflective to the 15 to 25 MHz acoustic signals than the surrounding kidney tissue. Males were recognized by the lack of the large non‐reflective ovary and, in some cases, the appearance of a small (0·2–0·3 mm) testis with slightly stronger reflective properties than the kidney. Identification of sex was confirmed by optical microscopy following dissection, and in a blind test on an additional 10 specimens (121–168 mm LT), the acoustic method reliably identified sex in 100% of the larvae. These results indicate that acoustic microscopy can determine the sex of live sea lamprey larvae in c. 30 s per animal, a process which until now required dissection or invasive surgery.</div>
</front>
</TEI>
<istex><corpusName>wiley</corpusName>
<author><json:item><name>E. Maeva</name>
<affiliations><json:string>Department of Physics, University of Windsor, Windsor, Ontario, N9B 3P4, Canada,</json:string>
</affiliations>
</json:item>
<json:item><name>I. Bruno</name>
<affiliations><json:string>Department of Physics, University of Windsor, Windsor, Ontario, N9B 3P4, Canada,</json:string>
</affiliations>
</json:item>
<json:item><name>B. S. Zielinski</name>
<affiliations><json:string>Department of Biological Sciences, University of Windsor, Windsor, Ontario, N9B 3P4, Canada and</json:string>
</affiliations>
</json:item>
<json:item><name>M. F. Docker</name>
<affiliations><json:string>Great Lakes Institute for Environmental Research, University of Windsor, Windsor, Ontario, N9B 3P4, Canada</json:string>
<json:string>E-mail: docker@uwindsor.ca</json:string>
</affiliations>
</json:item>
<json:item><name>F. M. Severin</name>
<affiliations><json:string>Department of Physics, University of Windsor, Windsor, Ontario, N9B 3P4, Canada,</json:string>
</affiliations>
</json:item>
<json:item><name>R. G. Maev</name>
<affiliations><json:string>Department of Physics, University of Windsor, Windsor, Ontario, N9B 3P4, Canada,</json:string>
</affiliations>
</json:item>
</author>
<subject><json:item><lang><json:string>eng</json:string>
</lang>
<value>acoustic microscopy</value>
</json:item>
<json:item><lang><json:string>eng</json:string>
</lang>
<value>Petromyzon marinus</value>
</json:item>
<json:item><lang><json:string>eng</json:string>
</lang>
<value>sex determination</value>
</json:item>
<json:item><lang><json:string>eng</json:string>
</lang>
<value>sea lamprey</value>
</json:item>
<json:item><lang><json:string>eng</json:string>
</lang>
<value>ultrasound</value>
</json:item>
</subject>
<articleId><json:string>JFB439</json:string>
</articleId>
<language><json:string>eng</json:string>
</language>
<originalGenre><json:string>article</json:string>
</originalGenre>
<abstract>A rapid, accurate and non‐invasive method to determine the sex of larval sea lampreys Petromyzon marinus, using wide‐field pulse‐scanning acoustic microscopy, is described. Cross‐sectional pulse‐echo scans were made with a high‐resolution acoustic microscope in 48 larvae (110–130 mm total length, LT), and the acoustic images generated showed such internal body structures as the gonad, intestine, kidneys, cardinal veins, notochord and musculature. Females were identified by the presence of a relatively large (1–1·5 mm diameter) ovary, which was considerably less reflective to the 15 to 25 MHz acoustic signals than the surrounding kidney tissue. Males were recognized by the lack of the large non‐reflective ovary and, in some cases, the appearance of a small (0·2–0·3 mm) testis with slightly stronger reflective properties than the kidney. Identification of sex was confirmed by optical microscopy following dissection, and in a blind test on an additional 10 specimens (121–168 mm LT), the acoustic method reliably identified sex in 100% of the larvae. These results indicate that acoustic microscopy can determine the sex of live sea lamprey larvae in c. 30 s per animal, a process which until now required dissection or invasive surgery.</abstract>
<qualityIndicators><score>7.077</score>
<pdfVersion>1.4</pdfVersion>
<pdfPageSize>488 x 703 pts</pdfPageSize>
<refBibsNative>true</refBibsNative>
<abstractCharCount>1248</abstractCharCount>
<pdfWordCount>4381</pdfWordCount>
<pdfCharCount>25790</pdfCharCount>
<pdfPageCount>9</pdfPageCount>
<abstractWordCount>183</abstractWordCount>
</qualityIndicators>
<title>The use of pulse‐echo acoustic microscopy to non‐invasively determine the sex of living larval sea lampreys</title>
<genre><json:string>article</json:string>
</genre>
<host><volume>65</volume>
<publisherId><json:string>JFB</json:string>
</publisherId>
<pages><total>9</total>
<last>156</last>
<first>148</first>
</pages>
<issn><json:string>0022-1112</json:string>
</issn>
<issue>1</issue>
<genre><json:string>journal</json:string>
</genre>
<language><json:string>unknown</json:string>
</language>
<eissn><json:string>1095-8649</json:string>
</eissn>
<title>Journal of Fish Biology</title>
<doi><json:string>10.1111/(ISSN)1095-8649</json:string>
</doi>
</host>
<categories><wos><json:string>science</json:string>
<json:string>marine & freshwater biology</json:string>
<json:string>fisheries</json:string>
</wos>
<scienceMetrix><json:string>applied sciences</json:string>
<json:string>agriculture, fisheries & forestry</json:string>
<json:string>fisheries</json:string>
</scienceMetrix>
</categories>
<publicationDate>2004</publicationDate>
<copyrightDate>2004</copyrightDate>
<doi><json:string>10.1111/j.0022-1112.2004.00439.x</json:string>
</doi>
<id>9E3FE084B7E812AD4C8DE1A5716DAA34D3F225FE</id>
<score>0.03111167</score>
<fulltext><json:item><extension>pdf</extension>
<original>true</original>
<mimetype>application/pdf</mimetype>
<uri>https://api.istex.fr/document/9E3FE084B7E812AD4C8DE1A5716DAA34D3F225FE/fulltext/pdf</uri>
</json:item>
<json:item><extension>zip</extension>
<original>false</original>
<mimetype>application/zip</mimetype>
<uri>https://api.istex.fr/document/9E3FE084B7E812AD4C8DE1A5716DAA34D3F225FE/fulltext/zip</uri>
</json:item>
<istex:fulltextTEI uri="https://api.istex.fr/document/9E3FE084B7E812AD4C8DE1A5716DAA34D3F225FE/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">The use of pulse‐echo acoustic microscopy to non‐invasively determine the sex of living larval sea lampreys</title>
</titleStmt>
<publicationStmt><authority>ISTEX</authority>
<publisher>Blackwell Science Ltd</publisher>
<pubPlace>Oxford, UK; Malden, USA</pubPlace>
<availability><p>WILEY</p>
</availability>
<date>2004</date>
</publicationStmt>
<sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">The use of pulse‐echo acoustic microscopy to non‐invasively determine the sex of living larval sea lampreys</title>
<author xml:id="author-1"><persName><forename type="first">E.</forename>
<surname>Maeva</surname>
</persName>
<affiliation>Department of Physics, University of Windsor, Windsor, Ontario, N9B 3P4, Canada,</affiliation>
</author>
<author xml:id="author-2"><persName><forename type="first">I.</forename>
<surname>Bruno</surname>
</persName>
<affiliation>Department of Physics, University of Windsor, Windsor, Ontario, N9B 3P4, Canada,</affiliation>
</author>
<author xml:id="author-3"><persName><forename type="first">B. S.</forename>
<surname>Zielinski</surname>
</persName>
<affiliation>Department of Biological Sciences, University of Windsor, Windsor, Ontario, N9B 3P4, Canada and</affiliation>
</author>
<author xml:id="author-4"><persName><forename type="first">M. F.</forename>
<surname>Docker</surname>
</persName>
<email>docker@uwindsor.ca</email>
<affiliation>Great Lakes Institute for Environmental Research, University of Windsor, Windsor, Ontario, N9B 3P4, Canada</affiliation>
</author>
<author xml:id="author-5"><persName><forename type="first">F. M.</forename>
<surname>Severin</surname>
</persName>
<affiliation>Department of Physics, University of Windsor, Windsor, Ontario, N9B 3P4, Canada,</affiliation>
</author>
<author xml:id="author-6"><persName><forename type="first">R. G.</forename>
<surname>Maev</surname>
</persName>
<affiliation>Department of Physics, University of Windsor, Windsor, Ontario, N9B 3P4, Canada,</affiliation>
</author>
</analytic>
<monogr><title level="j">Journal of Fish Biology</title>
<idno type="pISSN">0022-1112</idno>
<idno type="eISSN">1095-8649</idno>
<idno type="DOI">10.1111/(ISSN)1095-8649</idno>
<imprint><publisher>Blackwell Science Ltd</publisher>
<pubPlace>Oxford, UK; Malden, USA</pubPlace>
<date type="published" when="2004-07"></date>
<biblScope unit="volume">65</biblScope>
<biblScope unit="issue">1</biblScope>
<biblScope unit="page" from="148">148</biblScope>
<biblScope unit="page" to="156">156</biblScope>
</imprint>
</monogr>
<idno type="istex">9E3FE084B7E812AD4C8DE1A5716DAA34D3F225FE</idno>
<idno type="DOI">10.1111/j.0022-1112.2004.00439.x</idno>
<idno type="ArticleID">JFB439</idno>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><creation><date>2004</date>
</creation>
<langUsage><language ident="en">en</language>
</langUsage>
<abstract xml:lang="en"><p>A rapid, accurate and non‐invasive method to determine the sex of larval sea lampreys Petromyzon marinus, using wide‐field pulse‐scanning acoustic microscopy, is described. Cross‐sectional pulse‐echo scans were made with a high‐resolution acoustic microscope in 48 larvae (110–130 mm total length, LT), and the acoustic images generated showed such internal body structures as the gonad, intestine, kidneys, cardinal veins, notochord and musculature. Females were identified by the presence of a relatively large (1–1·5 mm diameter) ovary, which was considerably less reflective to the 15 to 25 MHz acoustic signals than the surrounding kidney tissue. Males were recognized by the lack of the large non‐reflective ovary and, in some cases, the appearance of a small (0·2–0·3 mm) testis with slightly stronger reflective properties than the kidney. Identification of sex was confirmed by optical microscopy following dissection, and in a blind test on an additional 10 specimens (121–168 mm LT), the acoustic method reliably identified sex in 100% of the larvae. These results indicate that acoustic microscopy can determine the sex of live sea lamprey larvae in c. 30 s per animal, a process which until now required dissection or invasive surgery.</p>
</abstract>
<textClass xml:lang="en"><keywords scheme="keyword"><list><head>keywords</head>
<item><term>acoustic microscopy</term>
</item>
<item><term>Petromyzon marinus</term>
</item>
<item><term>sex determination</term>
</item>
<item><term>sea lamprey</term>
</item>
<item><term>ultrasound</term>
</item>
</list>
</keywords>
</textClass>
</profileDesc>
<revisionDesc><change when="2004-07">Published</change>
</revisionDesc>
</teiHeader>
</istex:fulltextTEI>
<json:item><extension>txt</extension>
<original>false</original>
<mimetype>text/plain</mimetype>
<uri>https://api.istex.fr/document/9E3FE084B7E812AD4C8DE1A5716DAA34D3F225FE/fulltext/txt</uri>
</json:item>
</fulltext>
<metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration>
<istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>Blackwell Science Ltd</publisherName>
<publisherLoc>Oxford, UK; Malden, USA</publisherLoc>
</publisherInfo>
<doi origin="wiley" registered="yes">10.1111/(ISSN)1095-8649</doi>
<issn type="print">0022-1112</issn>
<issn type="electronic">1095-8649</issn>
<idGroup><id type="product" value="JFB"></id>
<id type="publisherDivision" value="ST"></id>
</idGroup>
<titleGroup><title type="main" sort="JOURNAL OF FISH BIOLOGY">Journal of Fish Biology</title>
</titleGroup>
</publicationMeta>
<publicationMeta level="part" position="07001"><doi origin="wiley">10.1111/jfb.2004.65.issue-1</doi>
<numberingGroup><numbering type="journalVolume" number="65">65</numbering>
<numbering type="journalIssue" number="1">1</numbering>
</numberingGroup>
<coverDate startDate="2004-07">July 2004</coverDate>
</publicationMeta>
<publicationMeta level="unit" type="article" position="0014800" status="forIssue"><doi origin="wiley">10.1111/j.0022-1112.2004.00439.x</doi>
<idGroup><id type="unit" value="JFB439"></id>
</idGroup>
<countGroup><count type="pageTotal" number="9"></count>
</countGroup>
<titleGroup><title type="tocHeading1">Regular Papers</title>
</titleGroup>
<eventGroup><event type="firstOnline" date="2004-07-02"></event>
<event type="publishedOnlineFinalForm" date="2004-07-02"></event>
<event type="xmlConverted" agent="Converter:BPG_TO_WML3G version:2.3.5 mode:FullText source:Header result:Header" date="2010-04-06"></event>
<event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:3.8.8" date="2014-01-30"></event>
<event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-10-30"></event>
</eventGroup>
<numberingGroup><numbering type="pageFirst" number="148">148</numbering>
<numbering type="pageLast" number="156">156</numbering>
</numberingGroup>
<correspondenceTo> §Tel.: +1 519 253 3000, ext. 3761; fax: +1 519 971 3616; email: <email>docker@uwindsor.ca</email>
</correspondenceTo>
<linkGroup><link type="toTypesetVersion" href="file:JFB.JFB439.pdf"></link>
</linkGroup>
</publicationMeta>
<contentMeta><unparsedEditorialHistory>(Received 17 June 2003, Accepted 30 March 2004)</unparsedEditorialHistory>
<countGroup><count type="figureTotal" number="2"></count>
<count type="tableTotal" number="0"></count>
<count type="formulaTotal" number="0"></count>
<count type="referenceTotal" number="33"></count>
<count type="wordTotal" number="0"></count>
<count type="linksPubMed" number="0"></count>
<count type="linksCrossRef" number="0"></count>
</countGroup>
<titleGroup><title type="main">The use of pulse‐echo acoustic microscopy to non‐invasively determine the sex of living larval sea lampreys</title>
<title type="shortAuthors"><sc>e. maeva <i>et al.</i>
</sc>
</title>
<title type="short"><sc>acoustic sex determination in live lampreys</sc>
</title>
</titleGroup>
<creators><creator creatorRole="author" xml:id="cr1" affiliationRef="#a1"><personName><givenNames>E.</givenNames>
<familyName>Maeva</familyName>
</personName>
</creator>
<creator creatorRole="author" xml:id="cr2" affiliationRef="#a1"><personName><givenNames>I.</givenNames>
<familyName>Bruno</familyName>
</personName>
</creator>
<creator creatorRole="author" xml:id="cr3" affiliationRef="#a2"><personName><givenNames>B. S.</givenNames>
<familyName>Zielinski</familyName>
</personName>
</creator>
<creator creatorRole="author" xml:id="cr4" affiliationRef="#a3" corresponding="yes"><personName><givenNames>M. F.</givenNames>
<familyName>Docker</familyName>
</personName>
</creator>
<creator creatorRole="author" xml:id="cr5" affiliationRef="#a1"><personName><givenNames>F. M.</givenNames>
<familyName>Severin</familyName>
</personName>
</creator>
<creator creatorRole="author" xml:id="cr6" affiliationRef="#a1"><personName><givenNames>R. G.</givenNames>
<familyName>Maev</familyName>
</personName>
</creator>
</creators>
<affiliationGroup><affiliation xml:id="a1" countryCode="CA"><unparsedAffiliation><i>Department of Physics, University of Windsor, Windsor, Ontario, N9B 3P4, Canada,</i>
</unparsedAffiliation>
</affiliation>
<affiliation xml:id="a2" countryCode="CA"><unparsedAffiliation><i>Department of Biological Sciences, University of Windsor, Windsor, Ontario, N9B 3P4, Canada and</i>
</unparsedAffiliation>
</affiliation>
<affiliation xml:id="a3" countryCode="CA"><unparsedAffiliation><i>Great Lakes Institute for Environmental Research, University of Windsor, Windsor, Ontario, N9B 3P4, Canada</i>
</unparsedAffiliation>
</affiliation>
</affiliationGroup>
<keywordGroup xml:lang="en"><keyword xml:id="k1">acoustic microscopy</keyword>
<keyword xml:id="k2"><i>Petromyzon marinus</i>
</keyword>
<keyword xml:id="k3">sex determination</keyword>
<keyword xml:id="k4">sea lamprey</keyword>
<keyword xml:id="k5">ultrasound</keyword>
</keywordGroup>
<abstractGroup><abstract type="main" xml:lang="en"><p>A rapid, accurate and non‐invasive method to determine the sex of larval sea lampreys <i>Petromyzon marinus</i>
, using wide‐field pulse‐scanning acoustic microscopy, is described. Cross‐sectional pulse‐echo scans were made with a high‐resolution acoustic microscope in 48 larvae (110–130 mm total length, <i>L</i>
<sub>T</sub>
), and the acoustic images generated showed such internal body structures as the gonad, intestine, kidneys, cardinal veins, notochord and musculature. Females were identified by the presence of a relatively large (1–1·5 mm diameter) ovary, which was considerably less reflective to the 15 to 25 MHz acoustic signals than the surrounding kidney tissue. Males were recognized by the lack of the large non‐reflective ovary and, in some cases, the appearance of a small (0·2–0·3 mm) testis with slightly stronger reflective properties than the kidney. Identification of sex was confirmed by optical microscopy following dissection, and in a blind test on an additional 10 specimens (121–168 mm <i>L</i>
<sub>T</sub>
), the acoustic method reliably identified sex in 100% of the larvae. These results indicate that acoustic microscopy can determine the sex of live sea lamprey larvae in <i>c</i>
. 30 s per animal, a process which until now required dissection or invasive surgery.</p>
</abstract>
</abstractGroup>
</contentMeta>
</header>
</component>
</istex:document>
</istex:metadataXml>
<mods version="3.6"><titleInfo lang="en"><title>The use of pulse‐echo acoustic microscopy to non‐invasively determine the sex of living larval sea lampreys</title>
</titleInfo>
<titleInfo type="abbreviated" lang="en"><title>acoustic sex determination in live lampreys</title>
</titleInfo>
<titleInfo type="alternative" contentType="CDATA" lang="en"><title>The use of pulse‐echo acoustic microscopy to non‐invasively determine the sex of living larval sea lampreys</title>
</titleInfo>
<name type="personal"><namePart type="given">E.</namePart>
<namePart type="family">Maeva</namePart>
<affiliation>Department of Physics, University of Windsor, Windsor, Ontario, N9B 3P4, Canada,</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">I.</namePart>
<namePart type="family">Bruno</namePart>
<affiliation>Department of Physics, University of Windsor, Windsor, Ontario, N9B 3P4, Canada,</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">B. S.</namePart>
<namePart type="family">Zielinski</namePart>
<affiliation>Department of Biological Sciences, University of Windsor, Windsor, Ontario, N9B 3P4, Canada and</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">M. F.</namePart>
<namePart type="family">Docker</namePart>
<affiliation>Great Lakes Institute for Environmental Research, University of Windsor, Windsor, Ontario, N9B 3P4, Canada</affiliation>
<affiliation>E-mail: docker@uwindsor.ca</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">F. M.</namePart>
<namePart type="family">Severin</namePart>
<affiliation>Department of Physics, University of Windsor, Windsor, Ontario, N9B 3P4, Canada,</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">R. G.</namePart>
<namePart type="family">Maev</namePart>
<affiliation>Department of Physics, University of Windsor, Windsor, Ontario, N9B 3P4, Canada,</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<typeOfResource>text</typeOfResource>
<genre type="article" displayLabel="article"></genre>
<originInfo><publisher>Blackwell Science Ltd</publisher>
<place><placeTerm type="text">Oxford, UK; Malden, USA</placeTerm>
</place>
<dateIssued encoding="w3cdtf">2004-07</dateIssued>
<edition>(Received 17 June 2003, Accepted 30 March 2004)</edition>
<copyrightDate encoding="w3cdtf">2004</copyrightDate>
</originInfo>
<language><languageTerm type="code" authority="rfc3066">en</languageTerm>
<languageTerm type="code" authority="iso639-2b">eng</languageTerm>
</language>
<physicalDescription><internetMediaType>text/html</internetMediaType>
<extent unit="figures">2</extent>
<extent unit="references">33</extent>
</physicalDescription>
<abstract lang="en">A rapid, accurate and non‐invasive method to determine the sex of larval sea lampreys Petromyzon marinus, using wide‐field pulse‐scanning acoustic microscopy, is described. Cross‐sectional pulse‐echo scans were made with a high‐resolution acoustic microscope in 48 larvae (110–130 mm total length, LT), and the acoustic images generated showed such internal body structures as the gonad, intestine, kidneys, cardinal veins, notochord and musculature. Females were identified by the presence of a relatively large (1–1·5 mm diameter) ovary, which was considerably less reflective to the 15 to 25 MHz acoustic signals than the surrounding kidney tissue. Males were recognized by the lack of the large non‐reflective ovary and, in some cases, the appearance of a small (0·2–0·3 mm) testis with slightly stronger reflective properties than the kidney. Identification of sex was confirmed by optical microscopy following dissection, and in a blind test on an additional 10 specimens (121–168 mm LT), the acoustic method reliably identified sex in 100% of the larvae. These results indicate that acoustic microscopy can determine the sex of live sea lamprey larvae in c. 30 s per animal, a process which until now required dissection or invasive surgery.</abstract>
<subject lang="en"><genre>keywords</genre>
<topic>acoustic microscopy</topic>
<topic>Petromyzon marinus</topic>
<topic>sex determination</topic>
<topic>sea lamprey</topic>
<topic>ultrasound</topic>
</subject>
<relatedItem type="host"><titleInfo><title>Journal of Fish Biology</title>
</titleInfo>
<genre type="journal">journal</genre>
<identifier type="ISSN">0022-1112</identifier>
<identifier type="eISSN">1095-8649</identifier>
<identifier type="DOI">10.1111/(ISSN)1095-8649</identifier>
<identifier type="PublisherID">JFB</identifier>
<part><date>2004</date>
<detail type="volume"><caption>vol.</caption>
<number>65</number>
</detail>
<detail type="issue"><caption>no.</caption>
<number>1</number>
</detail>
<extent unit="pages"><start>148</start>
<end>156</end>
<total>9</total>
</extent>
</part>
</relatedItem>
<identifier type="istex">9E3FE084B7E812AD4C8DE1A5716DAA34D3F225FE</identifier>
<identifier type="DOI">10.1111/j.0022-1112.2004.00439.x</identifier>
<identifier type="ArticleID">JFB439</identifier>
<recordInfo><recordContentSource>WILEY</recordContentSource>
<recordOrigin>Blackwell Science Ltd</recordOrigin>
</recordInfo>
</mods>
</metadata>
<serie></serie>
</istex>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Eau/explor/EsturgeonV1/Data/Istex/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000D88 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Corpus/biblio.hfd -nk 000D88 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Eau |area= EsturgeonV1 |flux= Istex |étape= Corpus |type= RBID |clé= ISTEX:9E3FE084B7E812AD4C8DE1A5716DAA34D3F225FE |texte= The use of pulse‐echo acoustic microscopy to non‐invasively determine the sex of living larval sea lampreys }}
![]() | This area was generated with Dilib version V0.6.27. | ![]() |