The use of pulse‐echo acoustic microscopy to non‐invasively determine the sex of living larval sea lampreys
Identifieur interne : 000D88 ( Istex/Corpus ); précédent : 000D87; suivant : 000D89The use of pulse‐echo acoustic microscopy to non‐invasively determine the sex of living larval sea lampreys
Auteurs : E. Maeva ; I. Bruno ; B. S. Zielinski ; M. F. Docker ; F. M. Severin ; R. G. MaevSource :
- Journal of Fish Biology [ 0022-1112 ] ; 2004-07.
English descriptors
Abstract
A rapid, accurate and non‐invasive method to determine the sex of larval sea lampreys Petromyzon marinus, using wide‐field pulse‐scanning acoustic microscopy, is described. Cross‐sectional pulse‐echo scans were made with a high‐resolution acoustic microscope in 48 larvae (110–130 mm total length, LT), and the acoustic images generated showed such internal body structures as the gonad, intestine, kidneys, cardinal veins, notochord and musculature. Females were identified by the presence of a relatively large (1–1·5 mm diameter) ovary, which was considerably less reflective to the 15 to 25 MHz acoustic signals than the surrounding kidney tissue. Males were recognized by the lack of the large non‐reflective ovary and, in some cases, the appearance of a small (0·2–0·3 mm) testis with slightly stronger reflective properties than the kidney. Identification of sex was confirmed by optical microscopy following dissection, and in a blind test on an additional 10 specimens (121–168 mm LT), the acoustic method reliably identified sex in 100% of the larvae. These results indicate that acoustic microscopy can determine the sex of live sea lamprey larvae in c. 30 s per animal, a process which until now required dissection or invasive surgery.
Url:
DOI: 10.1111/j.0022-1112.2004.00439.x
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ISTEX:9E3FE084B7E812AD4C8DE1A5716DAA34D3F225FELe document en format XML
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Cross‐sectional pulse‐echo scans were made with a high‐resolution acoustic microscope in 48 larvae (110–130 mm total length, <i>L</i><sub>T</sub>), and the acoustic images generated showed such internal body structures as the gonad, intestine, kidneys, cardinal veins, notochord and musculature. Females were identified by the presence of a relatively large (1–1·5 mm diameter) ovary, which was considerably less reflective to the 15 to 25 MHz acoustic signals than the surrounding kidney tissue. Males were recognized by the lack of the large non‐reflective ovary and, in some cases, the appearance of a small (0·2–0·3 mm) testis with slightly stronger reflective properties than the kidney. Identification of sex was confirmed by optical microscopy following dissection, and in a blind test on an additional 10 specimens (121–168 mm <i>L</i><sub>T</sub>), the acoustic method reliably identified sex in 100% of the larvae. These results indicate that acoustic microscopy can determine the sex of live sea lamprey larvae in <i>c</i>. 30 s per animal, a process which until now required dissection or invasive surgery.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>The use of pulse‐echo acoustic microscopy to non‐invasively determine the sex of living larval sea lampreys</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>acoustic sex determination in live lampreys</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>The use of pulse‐echo acoustic microscopy to non‐invasively determine the sex of living larval sea lampreys</title></titleInfo><name type="personal"><namePart type="given">E.</namePart><namePart type="family">Maeva</namePart><affiliation>Department of Physics, University of Windsor, Windsor, Ontario, N9B 3P4, Canada,</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">I.</namePart><namePart type="family">Bruno</namePart><affiliation>Department of Physics, University of Windsor, Windsor, Ontario, N9B 3P4, Canada,</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">B. S.</namePart><namePart type="family">Zielinski</namePart><affiliation>Department of Biological Sciences, University of Windsor, Windsor, Ontario, N9B 3P4, Canada and</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M. F.</namePart><namePart type="family">Docker</namePart><affiliation>Great Lakes Institute for Environmental Research, University of Windsor, Windsor, Ontario, N9B 3P4, Canada</affiliation><affiliation>E-mail: docker@uwindsor.ca</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">F. M.</namePart><namePart type="family">Severin</namePart><affiliation>Department of Physics, University of Windsor, Windsor, Ontario, N9B 3P4, Canada,</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">R. G.</namePart><namePart type="family">Maev</namePart><affiliation>Department of Physics, University of Windsor, Windsor, Ontario, N9B 3P4, Canada,</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Blackwell Science Ltd</publisher><place><placeTerm type="text">Oxford, UK; Malden, USA</placeTerm></place><dateIssued encoding="w3cdtf">2004-07</dateIssued><edition>(Received 17 June 2003, Accepted 30 March 2004)</edition><copyrightDate encoding="w3cdtf">2004</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">2</extent><extent unit="references">33</extent></physicalDescription><abstract lang="en">A rapid, accurate and non‐invasive method to determine the sex of larval sea lampreys Petromyzon marinus, using wide‐field pulse‐scanning acoustic microscopy, is described. Cross‐sectional pulse‐echo scans were made with a high‐resolution acoustic microscope in 48 larvae (110–130 mm total length, LT), and the acoustic images generated showed such internal body structures as the gonad, intestine, kidneys, cardinal veins, notochord and musculature. Females were identified by the presence of a relatively large (1–1·5 mm diameter) ovary, which was considerably less reflective to the 15 to 25 MHz acoustic signals than the surrounding kidney tissue. Males were recognized by the lack of the large non‐reflective ovary and, in some cases, the appearance of a small (0·2–0·3 mm) testis with slightly stronger reflective properties than the kidney. Identification of sex was confirmed by optical microscopy following dissection, and in a blind test on an additional 10 specimens (121–168 mm LT), the acoustic method reliably identified sex in 100% of the larvae. These results indicate that acoustic microscopy can determine the sex of live sea lamprey larvae in c. 30 s per animal, a process which until now required dissection or invasive surgery.</abstract><subject lang="en"><genre>keywords</genre><topic>acoustic microscopy</topic><topic>Petromyzon marinus</topic><topic>sex determination</topic><topic>sea lamprey</topic><topic>ultrasound</topic></subject><relatedItem type="host"><titleInfo><title>Journal of Fish Biology</title></titleInfo><genre type="journal">journal</genre><identifier type="ISSN">0022-1112</identifier><identifier type="eISSN">1095-8649</identifier><identifier type="DOI">10.1111/(ISSN)1095-8649</identifier><identifier type="PublisherID">JFB</identifier><part><date>2004</date><detail type="volume"><caption>vol.</caption><number>65</number></detail><detail type="issue"><caption>no.</caption><number>1</number></detail><extent unit="pages"><start>148</start><end>156</end><total>9</total></extent></part></relatedItem><identifier type="istex">9E3FE084B7E812AD4C8DE1A5716DAA34D3F225FE</identifier><identifier type="DOI">10.1111/j.0022-1112.2004.00439.x</identifier><identifier type="ArticleID">JFB439</identifier><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>Blackwell Science Ltd</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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