Improvement of chilled seabass sperm conservation using a cell culture medium
Identifieur interne : 000C30 ( Istex/Corpus ); précédent : 000C29; suivant : 000C31Improvement of chilled seabass sperm conservation using a cell culture medium
Auteurs : C. Fauvel ; S. Boryshpolets ; J. Cosson ; J. G. Wilson Leedy ; C. Labbé ; P. Haffray ; M. SuquetSource :
- Journal of Applied Ichthyology [ 0175-8659 ] ; 2012-12.
Abstract
The sperm of seabass is very fragile and it quickly loses its ability to fertilize after collection either if kept undiluted or in classic saline media. In order to avoid cryopreservation when only short conservation is required, the process of sperm management including sperm collection, sperm dilution rate in storage medium and storage medium composition, was subject to experimental trials. A concentration of 20% urine generated a low pH of seminal fluid, and it immediately altered the motility ability. However, pH did not seem to be the key agent of motility prevention since sperm dilution in Leibovitz culture medium (L15) or in classic saline medium both presenting a similar low pH (7.3) did not affect motility. L15 increased the duration of sperm survival by 2 days at 4°C after collection. Moreover, dilution could be restricted to 1 : 3 (v : v) for conservation of chilled sperm. Chilled sperm could be cryopreserved with no more damages than those observed after freezing of fresh sperm.
Url:
DOI: 10.1111/jai.12071
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ISTEX:5413E02DE5C303416ED8330BBDA977E4B53E2BEALe document en format XML
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Fauvel</name><affiliation><mods:affiliation>Ifremer, UMR 5119 ECOSYM, UM2 – UM1 – CNRS – IRD – IFREMER, Station Expérimentale d'Aquaculture, 34250, Palavas, France</mods:affiliation></affiliation><affiliation><mods:affiliation>Christian Fauvel, Ifremer, UMR 5119 ECOSYM, UM2 – UM1 – CNRS – IRD – IFREMER, Station Expérimentale d'Aquaculture, Route de Maguelone 34250 Palavas, France.E‐mail:</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: Christian.Fauvel@ifremer.fr</mods:affiliation></affiliation></author><author><name sortKey="Boryshpolets, S" sort="Boryshpolets, S" uniqKey="Boryshpolets S" first="S." last="Boryshpolets">S. Boryshpolets</name><affiliation><mods:affiliation>Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Vodnany, Czech Republic</mods:affiliation></affiliation></author><author><name sortKey="Cosson, J" sort="Cosson, J" uniqKey="Cosson J" first="J." last="Cosson">J. Cosson</name><affiliation><mods:affiliation>Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Vodnany, Czech Republic</mods:affiliation></affiliation></author><author><name sortKey="Wilson Leedy, J G" sort="Wilson Leedy, J G" uniqKey="Wilson Leedy J" first="J. G." last="Wilson Leedy">J. G. Wilson Leedy</name><affiliation><mods:affiliation>Department of Biological Sciences, University of Idaho, Center for reproductive biology, ID, Moscow, USA</mods:affiliation></affiliation></author><author><name sortKey="Labbe, C" sort="Labbe, C" uniqKey="Labbe C" first="C." last="Labbé">C. Labbé</name><affiliation><mods:affiliation>INRA, UR 1037 Scribe, Rennes, France</mods:affiliation></affiliation></author><author><name sortKey="Haffray, P" sort="Haffray, P" uniqKey="Haffray P" first="P." last="Haffray">P. Haffray</name><affiliation><mods:affiliation>SYSAAF, Scribe, Rennes, France</mods:affiliation></affiliation></author><author><name sortKey="Suquet, M" sort="Suquet, M" uniqKey="Suquet M" first="M." last="Suquet">M. Suquet</name><affiliation><mods:affiliation>Ifremer, UMR 100, Station Expérimentale d'Argenton, Argenton, France</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Applied Ichthyology</title><title level="j" type="abbrev">J. Appl. 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In order to avoid cryopreservation when only short conservation is required, the process of sperm management including sperm collection, sperm dilution rate in storage medium and storage medium composition, was subject to experimental trials. A concentration of 20% urine generated a low pH of seminal fluid, and it immediately altered the motility ability. However, pH did not seem to be the key agent of motility prevention since sperm dilution in Leibovitz culture medium (L15) or in classic saline medium both presenting a similar low pH (7.3) did not affect motility. L15 increased the duration of sperm survival by 2 days at 4°C after collection. Moreover, dilution could be restricted to 1 : 3 (v : v) for conservation of chilled sperm. Chilled sperm could be cryopreserved with no more damages than those observed after freezing of fresh sperm.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>C. 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However, pH did not seem to be the key agent of motility prevention since sperm dilution in Leibovitz culture medium (L15) or in classic saline medium both presenting a similar low pH (7.3) did not affect motility. L15 increased the duration of sperm survival by 2 days at 4°C after collection. Moreover, dilution could be restricted to 1 : 3 (v : v) for conservation of chilled sperm. 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G.</forename><surname>Wilson Leedy</surname></persName><affiliation>Department of Biological Sciences, University of Idaho, Center for reproductive biology, ID, Moscow, USA</affiliation></author><author xml:id="author-5"><persName><forename type="first">C.</forename><surname>Labbé</surname></persName><affiliation>INRA, UR 1037 Scribe, Rennes, France</affiliation></author><author xml:id="author-6"><persName><forename type="first">P.</forename><surname>Haffray</surname></persName><affiliation>SYSAAF, Scribe, Rennes, France</affiliation></author><author xml:id="author-7"><persName><forename type="first">M.</forename><surname>Suquet</surname></persName><affiliation>Ifremer, UMR 100, Station Expérimentale d'Argenton, Argenton, France</affiliation></author></analytic><monogr><title level="j">Journal of Applied Ichthyology</title><title level="j" type="abbrev">J. Appl. Ichthyol.</title><idno type="pISSN">0175-8659</idno><idno type="eISSN">1439-0426</idno><idno type="DOI">10.1111/(ISSN)1439-0426</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><date type="published" when="2012-12"></date><biblScope unit="volume">28</biblScope><biblScope unit="issue">6</biblScope><biblScope unit="page" from="961">961</biblScope><biblScope unit="page" to="966">966</biblScope></imprint></monogr><idno type="istex">5413E02DE5C303416ED8330BBDA977E4B53E2BEA</idno><idno type="DOI">10.1111/jai.12071</idno><idno type="ArticleID">JAI12071</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2012-09-28</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>The sperm of seabass is very fragile and it quickly loses its ability to fertilize after collection either if kept undiluted or in classic saline media. In order to avoid cryopreservation when only short conservation is required, the process of sperm management including sperm collection, sperm dilution rate in storage medium and storage medium composition, was subject to experimental trials. A concentration of 20% urine generated a low pH of seminal fluid, and it immediately altered the motility ability. However, pH did not seem to be the key agent of motility prevention since sperm dilution in Leibovitz culture medium (L15) or in classic saline medium both presenting a similar low pH (7.3) did not affect motility. L15 increased the duration of sperm survival by 2 days at 4°C after collection. Moreover, dilution could be restricted to 1 : 3 (v : v) for conservation of chilled sperm. Chilled sperm could be cryopreserved with no more damages than those observed after freezing of fresh sperm.</p></abstract><textClass><keywords scheme="Journal Subject"><list><head>article-category</head><item><term>Original Article</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2012-05-10">Received</change><change when="2012-08-10">Registration</change><change when="2012-09-28">Created</change><change when="2012-12">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/5413E02DE5C303416ED8330BBDA977E4B53E2BEA/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component type="serialArticle" version="2.0" xml:id="jai12071" xml:lang="en"><header><publicationMeta level="product"><doi origin="wiley" registered="yes">10.1111/(ISSN)1439-0426</doi><issn type="print">0175-8659</issn><issn type="electronic">1439-0426</issn><idGroup><id type="product" value="JAI"></id></idGroup><titleGroup><title sort="JOURNAL OF APPLIED ICHTHYOLOGY" type="main">Journal of Applied Ichthyology</title><title type="short">J. 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Fauvel et al.</title></titleGroup><creators><creator affiliationRef="#jai12071-aff-0001" corresponding="yes" creatorRole="author" xml:id="jai12071-cr-0001"><personName><givenNames>C.</givenNames> <familyName>Fauvel</familyName></personName></creator><creator affiliationRef="#jai12071-aff-0002" creatorRole="author" xml:id="jai12071-cr-0002"><personName><givenNames>S.</givenNames> <familyName>Boryshpolets</familyName></personName></creator><creator affiliationRef="#jai12071-aff-0002" creatorRole="author" xml:id="jai12071-cr-0003"><personName><givenNames>J.</givenNames> <familyName>Cosson</familyName></personName></creator><creator affiliationRef="#jai12071-aff-0003" creatorRole="author" xml:id="jai12071-cr-0004"><personName><givenNames>J. G.</givenNames> <familyName>Wilson Leedy</familyName></personName></creator><creator affiliationRef="#jai12071-aff-0004" creatorRole="author" xml:id="jai12071-cr-0005"><personName><givenNames>C.</givenNames> <familyName>Labbé</familyName></personName></creator><creator affiliationRef="#jai12071-aff-0005" creatorRole="author" xml:id="jai12071-cr-0006"><personName><givenNames>P.</givenNames> <familyName>Haffray</familyName></personName></creator><creator affiliationRef="#jai12071-aff-0006" creatorRole="author" xml:id="jai12071-cr-0007"><personName><givenNames>M.</givenNames> <familyName>Suquet</familyName></personName></creator></creators><affiliationGroup><affiliation countryCode="FR" type="organization" xml:id="jai12071-aff-0001"><orgDiv>Ifremer</orgDiv> <orgDiv>UMR 5119 ECOSYM, UM2 – UM1 – CNRS – IRD – IFREMER</orgDiv> <orgName>Station Expérimentale d'Aquaculture</orgName> <address><city>34250, Palavas</city> <country>France</country></address></affiliation><affiliation countryCode="CZ" type="organization" xml:id="jai12071-aff-0002"><orgDiv>Faculty of Fisheries and Protection of Waters</orgDiv> <orgName>University of South Bohemia in Ceske Budejovice</orgName> <orgName>South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses</orgName> <address><city>Vodnany</city> <country>Czech Republic</country></address></affiliation><affiliation countryCode="US" type="organization" xml:id="jai12071-aff-0003"><orgDiv>Department of Biological Sciences</orgDiv> <orgName>University of Idaho</orgName> <orgName>Center for reproductive biology</orgName> <address><city>Moscow</city> <countryPart>ID</countryPart> <country>USA</country></address></affiliation><affiliation countryCode="FR" type="organization" xml:id="jai12071-aff-0004"><orgDiv>INRA</orgDiv> <orgName>UR 1037 Scribe</orgName> <address><city>Rennes</city> <country>France</country></address></affiliation><affiliation countryCode="FR" type="organization" xml:id="jai12071-aff-0005"><orgName>SYSAAF</orgName> <orgName>Scribe</orgName> <address><city>Rennes</city> <country>France</country></address></affiliation><affiliation countryCode="FR" type="organization" xml:id="jai12071-aff-0006"><orgDiv>Ifremer</orgDiv> <orgName>UMR 100</orgName> <orgName>Station Expérimentale d'Argenton</orgName> <address><city>Argenton</city> <country>France</country></address></affiliation></affiliationGroup><abstractGroup><abstract type="main" xml:id="jai12071-abs-0001" xml:lang="en"><title type="main">Summary</title><p>The sperm of seabass is very fragile and it quickly loses its ability to fertilize after collection either if kept undiluted or in classic saline media. In order to avoid cryopreservation when only short conservation is required, the process of sperm management including sperm collection, sperm dilution rate in storage medium and storage medium composition, was subject to experimental trials. A concentration of 20% urine generated a low <fc>pH</fc> of seminal fluid, and it immediately altered the motility ability. However, <fc>pH</fc> did not seem to be the key agent of motility prevention since sperm dilution in Leibovitz culture medium (L15) or in classic saline medium both presenting a similar low <fc>pH</fc> (7.3) did not affect motility. L15 increased the duration of sperm survival by 2 days at 4°C after collection. Moreover, dilution could be restricted to 1 : 3 (v : v) for conservation of chilled sperm. Chilled sperm could be cryopreserved with no more damages than those observed after freezing of fresh sperm.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Improvement of chilled seabass sperm conservation using a cell culture medium</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Improvement of chilled seabass sperm conservation using a cell culture medium</title></titleInfo><name type="personal"><namePart type="given">C.</namePart><namePart type="family">Fauvel</namePart><affiliation>Ifremer, UMR 5119 ECOSYM, UM2 – UM1 – CNRS – IRD – IFREMER, Station Expérimentale d'Aquaculture, 34250, Palavas, France</affiliation><affiliation>Christian Fauvel, Ifremer, UMR 5119 ECOSYM, UM2 – UM1 – CNRS – IRD – IFREMER, Station Expérimentale d'Aquaculture, Route de Maguelone 34250 Palavas, France.E‐mail:</affiliation><affiliation>E-mail: Christian.Fauvel@ifremer.fr</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S.</namePart><namePart type="family">Boryshpolets</namePart><affiliation>Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Vodnany, Czech Republic</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J.</namePart><namePart type="family">Cosson</namePart><affiliation>Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Vodnany, Czech Republic</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J. G.</namePart><namePart type="family">Wilson Leedy</namePart><affiliation>Department of Biological Sciences, University of Idaho, Center for reproductive biology, ID, Moscow, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">C.</namePart><namePart type="family">Labbé</namePart><affiliation>INRA, UR 1037 Scribe, Rennes, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">P.</namePart><namePart type="family">Haffray</namePart><affiliation>SYSAAF, Scribe, Rennes, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Suquet</namePart><affiliation>Ifremer, UMR 100, Station Expérimentale d'Argenton, Argenton, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Blackwell Publishing Ltd</publisher><dateIssued encoding="w3cdtf">2012-12</dateIssued><dateCreated encoding="w3cdtf">2012-09-28</dateCreated><dateCaptured encoding="w3cdtf">2012-05-10</dateCaptured><dateValid encoding="w3cdtf">2012-08-10</dateValid><copyrightDate encoding="w3cdtf">2012</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">The sperm of seabass is very fragile and it quickly loses its ability to fertilize after collection either if kept undiluted or in classic saline media. In order to avoid cryopreservation when only short conservation is required, the process of sperm management including sperm collection, sperm dilution rate in storage medium and storage medium composition, was subject to experimental trials. A concentration of 20% urine generated a low pH of seminal fluid, and it immediately altered the motility ability. However, pH did not seem to be the key agent of motility prevention since sperm dilution in Leibovitz culture medium (L15) or in classic saline medium both presenting a similar low pH (7.3) did not affect motility. L15 increased the duration of sperm survival by 2 days at 4°C after collection. Moreover, dilution could be restricted to 1 : 3 (v : v) for conservation of chilled sperm. Chilled sperm could be cryopreserved with no more damages than those observed after freezing of fresh sperm.</abstract><relatedItem type="host"><titleInfo><title>Journal of Applied Ichthyology</title></titleInfo><titleInfo type="abbreviated"><title>J. Appl. Ichthyol.</title></titleInfo><genre type="journal">journal</genre><subject><genre>article-category</genre><topic>Original Article</topic></subject><identifier type="ISSN">0175-8659</identifier><identifier type="eISSN">1439-0426</identifier><identifier type="DOI">10.1111/(ISSN)1439-0426</identifier><identifier type="PublisherID">JAI</identifier><part><date>2012</date><detail type="title"><title>Proceedings of the Third International Workshop on the “Biology of Fish Gametes” : Budapest, Hungary, 7–9 September 2011</title></detail><detail type="volume"><caption>vol.</caption><number>28</number></detail><detail type="issue"><caption>no.</caption><number>6</number></detail><extent unit="pages"><start>961</start><end>966</end><total>6</total></extent></part></relatedItem><identifier type="istex">5413E02DE5C303416ED8330BBDA977E4B53E2BEA</identifier><identifier type="DOI">10.1111/jai.12071</identifier><identifier type="ArticleID">JAI12071</identifier><accessCondition type="use and reproduction" contentType="copyright">Copyright © 2012 Blackwell Verlag GmbH, Wiley‐Blackwell, Rotherstraße 21, 10245 Berlin, Germany© 2012 Blackwell Verlag GmbH</accessCondition><recordInfo><recordContentSource>WILEY</recordContentSource></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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