Central nervous system myelin proteins of the coelacanth Latimeria chalumnae: phylogenetic implications
Identifieur interne : 000C04 ( Istex/Corpus ); précédent : 000C03; suivant : 000C05Central nervous system myelin proteins of the coelacanth Latimeria chalumnae: phylogenetic implications
Auteurs : Thomas V. Waehneldt ; Joachim Malotka ; Gunnar Jeserich ; Jean-Marie MatthieuSource :
- Environmental Biology of Fishes [ 0378-1909 ] ; 1991-09-01.
Abstract
Synopsis: Myelin was isolated from the brain of a coelacanth. Its protein components were separated by polyacrylamide gel electrophoresis in the presence of sodium dodecylsulfate (SDS-PAGE). A protein component of 25000 Dalton was predominant; it was not glycosylated but reacted moderately with anti-mammalian CNS myelin proteolipid protein (PLP) antibodies and weakly with anti-lungfish CNS myelin glycosylated proteolipid protein (gPLP) antibodies. A component equivalent to mammalian DM-20 was not detectable. Presumably due to autolysis myelin basic protein (MBP) was not discernible by protein staining but showed up as a single band of 17000 Dalton with anti-mammalian MBP antibodies. Wolfgram protein (WP) was not present upon immunoblotting and the values for the myelin-specific 2′, 3′-cyclic nucleotide 3′-phosphodiesterase (CNPase) were extremely low. These results question a chondrichthyan association of the coelacanth but are strongly in favor of an Actinistia-Tetrapoda sister group relationship, with Dipnoi being most closely related to that combined group.
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DOI: 10.1007/BF00007449
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<affiliation>Max-Planck-Institut für experimentelle Medizin, Forschungsstelle Neurochemie, D-3400, Göttingen, Germany</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">Joachim</namePart>
<namePart type="family">Malotka</namePart>
<affiliation>Max-Planck-Institut für experimentelle Medizin, Forschungsstelle Neurochemie, D-3400, Göttingen, Germany</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">Gunnar</namePart>
<namePart type="family">Jeserich</namePart>
<affiliation>Zoophysiologie/Zellphysiologie, Universität Osnabrück, D-4500, Osnabrück, Germany</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">Jean-Marie</namePart>
<namePart type="family">Matthieu</namePart>
<affiliation>Laboratoire de Neurochimie, Service de Pédiatrie, CHUV, CH-1011, Lausanne, Switzerland</affiliation>
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<typeOfResource>text</typeOfResource>
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<originInfo><publisher>Kluwer Academic Publishers</publisher>
<place><placeTerm type="text">Dordrecht</placeTerm>
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<dateCreated encoding="w3cdtf">1989-03-01</dateCreated>
<dateIssued encoding="w3cdtf">1991-09-01</dateIssued>
<copyrightDate encoding="w3cdtf">1991</copyrightDate>
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<language><languageTerm type="code" authority="rfc3066">en</languageTerm>
<languageTerm type="code" authority="iso639-2b">eng</languageTerm>
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<abstract lang="en">Synopsis: Myelin was isolated from the brain of a coelacanth. Its protein components were separated by polyacrylamide gel electrophoresis in the presence of sodium dodecylsulfate (SDS-PAGE). A protein component of 25000 Dalton was predominant; it was not glycosylated but reacted moderately with anti-mammalian CNS myelin proteolipid protein (PLP) antibodies and weakly with anti-lungfish CNS myelin glycosylated proteolipid protein (gPLP) antibodies. A component equivalent to mammalian DM-20 was not detectable. Presumably due to autolysis myelin basic protein (MBP) was not discernible by protein staining but showed up as a single band of 17000 Dalton with anti-mammalian MBP antibodies. Wolfgram protein (WP) was not present upon immunoblotting and the values for the myelin-specific 2′, 3′-cyclic nucleotide 3′-phosphodiesterase (CNPase) were extremely low. These results question a chondrichthyan association of the coelacanth but are strongly in favor of an Actinistia-Tetrapoda sister group relationship, with Dipnoi being most closely related to that combined group.</abstract>
<relatedItem type="host"><titleInfo><title>Environmental Biology of Fishes</title>
</titleInfo>
<titleInfo type="abbreviated"><title>Environ Biol Fish</title>
</titleInfo>
<genre type="journal" displayLabel="Archive Journal"></genre>
<originInfo><dateIssued encoding="w3cdtf">1991-09-01</dateIssued>
<copyrightDate encoding="w3cdtf">1991</copyrightDate>
</originInfo>
<subject><genre>Environment</genre>
<topic>Hydrobiology</topic>
<topic>Zoology</topic>
<topic>Animal Systematics/Taxonomy/Biogeography</topic>
<topic>Environment, general</topic>
<topic>Nature Conservation</topic>
</subject>
<identifier type="ISSN">0378-1909</identifier>
<identifier type="eISSN">1573-5133</identifier>
<identifier type="JournalID">10641</identifier>
<identifier type="IssueArticleCount">31</identifier>
<identifier type="VolumeIssueCount">4</identifier>
<part><date>1991</date>
<detail type="volume"><number>32</number>
<caption>vol.</caption>
</detail>
<detail type="issue"><number>1-4</number>
<caption>no.</caption>
</detail>
<extent unit="pages"><start>131</start>
<end>143</end>
</extent>
</part>
<recordInfo><recordOrigin>Kluwer Academic Publishers, 1991</recordOrigin>
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<identifier type="istex">731C7B7ED94487D4B28748C6D2297628C3177AA6</identifier>
<identifier type="DOI">10.1007/BF00007449</identifier>
<identifier type="ArticleID">BF00007449</identifier>
<identifier type="ArticleID">Art8</identifier>
<accessCondition type="use and reproduction" contentType="copyright">Kluwer Academic Publishers, 1991</accessCondition>
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<recordOrigin>Kluwer Academic Publishers, 1991</recordOrigin>
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