Identification and characterization of maturation-promoting factor from catfish, Clarias batrachus
Identifieur interne : 000B71 ( Istex/Corpus ); précédent : 000B70; suivant : 000B72Identification and characterization of maturation-promoting factor from catfish, Clarias batrachus
Auteurs : S. Haider ; K. BalamuruganSource :
- Fish Physiology and Biochemistry [ 0920-1742 ] ; 1996-06-01.
Abstract
Abstract: Maturation-promoting factor (MPF) extracted from maturing oocytes of catfishes (Clarias batrachus andHeteropneustes fossilis) and carp (Labeo rohita) induces 100% germinal vesicle breakdown (GVBD) when microinjected intoClarias immature unstimulated oocytes. The presence of a similar MPF activity has also been demonstrated in the active fractions collected after superose 12. SDS-PAGE analyses of cytosolic extracts (CE) prepared from immature and mature oocytes revealed the presence of 34- and 46 kDa proteins apart from a few others. Antibody against the PSTAIR sequence of p34cdc2 recognized 32- and 34 kDa proteins of immature as well as mature oocytes while, 46 kDa protein of mature oocytes was recognized by anti-cyclin B1 antibody. Moreover, labelling of [35S]methionine was observed mainly in the region of 46 kDa protein band indicatingde novo synthesis of this particular protein. Anti-cyclin A antibody did not recognize any proteins of immature or mature oocytes. Cyclin B1 was absent in immature oocytes and ovulated eggs. These findings indicate the presence of p34cdc2 homologs and cyclin B in the MPF of the catfishes and carp oocytes.
Url:
DOI: 10.1007/BF01875576
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The presence of a similar MPF activity has also been demonstrated in the active fractions collected after superose 12. SDS-PAGE analyses of cytosolic extracts (CE) prepared from immature and mature oocytes revealed the presence of 34- and 46 kDa proteins apart from a few others. Antibody against the PSTAIR sequence of p34cdc2 recognized 32- and 34 kDa proteins of immature as well as mature oocytes while, 46 kDa protein of mature oocytes was recognized by anti-cyclin B1 antibody. Moreover, labelling of [35S]methionine was observed mainly in the region of 46 kDa protein band indicatingde novo synthesis of this particular protein. Anti-cyclin A antibody did not recognize any proteins of immature or mature oocytes. Cyclin B1 was absent in immature oocytes and ovulated eggs. These findings indicate the presence of p34cdc2 homologs and cyclin B in the MPF of the catfishes and carp oocytes.</div></front></TEI><istex><corpusName>springer</corpusName><author><json:item><name>S. 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SDS-PAGE analyses of cytosolic extracts (CE) prepared from immature and mature oocytes revealed the presence of 34- and 46 kDa proteins apart from a few others. Antibody against the PSTAIR sequence of p34cdc2 recognized 32- and 34 kDa proteins of immature as well as mature oocytes while, 46 kDa protein of mature oocytes was recognized by anti-cyclin B1 antibody. Moreover, labelling of [35S]methionine was observed mainly in the region of 46 kDa protein band indicatingde novo synthesis of this particular protein. Anti-cyclin A antibody did not recognize any proteins of immature or mature oocytes. Cyclin B1 was absent in immature oocytes and ovulated eggs. 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Physiology and Biochemistry</JournalTitle><JournalAbbreviatedTitle>Fish Physiol Biochem</JournalAbbreviatedTitle><JournalSubjectGroup><JournalSubject Type="Primary">Life Sciences</JournalSubject><JournalSubject Type="Secondary">Animal Biochemistry</JournalSubject><JournalSubject Type="Secondary">Hydrobiology</JournalSubject><JournalSubject Type="Secondary">Zoology</JournalSubject><JournalSubject Type="Secondary">Animal Anatomy / Morphology / Histology</JournalSubject><JournalSubject Type="Secondary">Animal Physiology</JournalSubject></JournalSubjectGroup></JournalInfo><Volume><VolumeInfo VolumeType="Regular" TocLevels="0"><VolumeIDStart>15</VolumeIDStart><VolumeIDEnd>15</VolumeIDEnd><VolumeIssueCount>6</VolumeIssueCount></VolumeInfo><Issue IssueType="Regular"><IssueInfo 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batrachus</Emphasis></ArticleTitle><ArticleFirstPage>255</ArticleFirstPage><ArticleLastPage>263</ArticleLastPage><ArticleHistory><RegistrationDate><Year>2005</Year><Month>6</Month><Day>8</Day></RegistrationDate><Accepted><Year>1995</Year><Month>11</Month><Day>24</Day></Accepted></ArticleHistory><ArticleCopyright><CopyrightHolderName>Kugler Publication</CopyrightHolderName><CopyrightYear>1996</CopyrightYear></ArticleCopyright><ArticleGrants Type="Regular"><MetadataGrant Grant="OpenAccess"></MetadataGrant><AbstractGrant Grant="OpenAccess"></AbstractGrant><BodyPDFGrant Grant="Restricted"></BodyPDFGrant><BodyHTMLGrant Grant="Restricted"></BodyHTMLGrant><BibliographyGrant Grant="Restricted"></BibliographyGrant><ESMGrant Grant="Restricted"></ESMGrant></ArticleGrants><ArticleContext><JournalID>10695</JournalID><VolumeIDStart>15</VolumeIDStart><VolumeIDEnd>15</VolumeIDEnd><IssueIDStart>3</IssueIDStart><IssueIDEnd>3</IssueIDEnd></ArticleContext></ArticleInfo><ArticleHeader><AuthorGroup><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>S.</GivenName><FamilyName>Haider</FamilyName></AuthorName></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>K.</GivenName><FamilyName>Balamurugan</FamilyName></AuthorName></Author><Affiliation ID="Aff1"><OrgDivision>Department of Zoology</OrgDivision><OrgName>Banaras Hindu University</OrgName><OrgAddress><Postcode>221 005</Postcode><City>Varanasi</City><Country>India</Country></OrgAddress></Affiliation></AuthorGroup><Abstract ID="Abs1" Language="En"><Heading>Abstract</Heading><Para>Maturation-promoting factor (MPF) extracted from maturing oocytes of catfishes (<Emphasis Type="Italic">Clarias batrachus</Emphasis> and<Emphasis Type="Italic">Heteropneustes fossilis</Emphasis>) and carp (<Emphasis Type="Italic">Labeo rohita</Emphasis>) induces 100% germinal vesicle breakdown (GVBD) when microinjected into<Emphasis Type="Italic">Clarias</Emphasis> immature unstimulated oocytes. The presence of a similar MPF activity has also been demonstrated in the active fractions collected after superose 12. SDS-PAGE analyses of cytosolic extracts (CE) prepared from immature and mature oocytes revealed the presence of 34- and 46 kDa proteins apart from a few others. Antibody against the PSTAIR sequence of p34<Superscript>cdc2</Superscript> recognized 32- and 34 kDa proteins of immature as well as mature oocytes while, 46 kDa protein of mature oocytes was recognized by anti-cyclin B1 antibody. Moreover, labelling of [<Superscript>35</Superscript>S]methionine was observed mainly in the region of 46 kDa protein band indicating<Emphasis Type="Italic">de novo</Emphasis> synthesis of this particular protein. Anti-cyclin A antibody did not recognize any proteins of immature or mature oocytes. Cyclin B1 was absent in immature oocytes and ovulated eggs. These findings indicate the presence of p34<Superscript>cdc2</Superscript> homologs and cyclin B in the MPF of the catfishes and carp oocytes.</Para></Abstract><KeywordGroup Language="En"><Heading>Keywords</Heading><Keyword>catfish</Keyword><Keyword>maturation-promoting factor</Keyword><Keyword>identification</Keyword><Keyword>characterization</Keyword></KeywordGroup></ArticleHeader><NoBody></NoBody></Article></Issue></Volume></Journal></Publisher></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Identification and characterization of maturation-promoting factor from catfish, Clarias batrachus</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Identification and characterization of maturation-promoting factor from catfish,Clarias batrachus</title></titleInfo><name type="personal"><namePart type="given">S.</namePart><namePart type="family">Haider</namePart><affiliation>Department of Zoology, Banaras Hindu University, 221 005, Varanasi, India</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">K.</namePart><namePart type="family">Balamurugan</namePart><affiliation>Department of Zoology, Banaras Hindu University, 221 005, Varanasi, India</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="OriginalPaper"></genre><originInfo><publisher>Kluwer Academic Publishers</publisher><place><placeTerm type="text">Dordrecht</placeTerm></place><dateIssued encoding="w3cdtf">1996-06-01</dateIssued><copyrightDate encoding="w3cdtf">1996</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">Abstract: Maturation-promoting factor (MPF) extracted from maturing oocytes of catfishes (Clarias batrachus andHeteropneustes fossilis) and carp (Labeo rohita) induces 100% germinal vesicle breakdown (GVBD) when microinjected intoClarias immature unstimulated oocytes. The presence of a similar MPF activity has also been demonstrated in the active fractions collected after superose 12. SDS-PAGE analyses of cytosolic extracts (CE) prepared from immature and mature oocytes revealed the presence of 34- and 46 kDa proteins apart from a few others. Antibody against the PSTAIR sequence of p34cdc2 recognized 32- and 34 kDa proteins of immature as well as mature oocytes while, 46 kDa protein of mature oocytes was recognized by anti-cyclin B1 antibody. Moreover, labelling of [35S]methionine was observed mainly in the region of 46 kDa protein band indicatingde novo synthesis of this particular protein. Anti-cyclin A antibody did not recognize any proteins of immature or mature oocytes. Cyclin B1 was absent in immature oocytes and ovulated eggs. These findings indicate the presence of p34cdc2 homologs and cyclin B in the MPF of the catfishes and carp oocytes.</abstract><relatedItem type="host"><titleInfo><title>Fish Physiology and Biochemistry</title></titleInfo><titleInfo type="abbreviated"><title>Fish Physiol Biochem</title></titleInfo><genre type="journal" displayLabel="Archive Journal"></genre><originInfo><dateIssued encoding="w3cdtf">1996-06-01</dateIssued><copyrightDate encoding="w3cdtf">1996</copyrightDate></originInfo><subject><genre>Life Sciences</genre><topic>Animal Biochemistry</topic><topic>Hydrobiology</topic><topic>Zoology</topic><topic>Animal Anatomy / Morphology / Histology</topic><topic>Animal Physiology</topic></subject><identifier type="ISSN">0920-1742</identifier><identifier type="eISSN">1573-5168</identifier><identifier type="JournalID">10695</identifier><identifier type="IssueArticleCount">9</identifier><identifier type="VolumeIssueCount">6</identifier><part><date>1996</date><detail type="volume"><number>15</number><caption>vol.</caption></detail><detail type="issue"><number>3</number><caption>no.</caption></detail><extent unit="pages"><start>255</start><end>263</end></extent></part><recordInfo><recordOrigin>Kugler Publication, 1996</recordOrigin></recordInfo></relatedItem><identifier type="istex">81B44E4FAE0AB61A2A686D590EC9AF6593C97ABF</identifier><identifier type="DOI">10.1007/BF01875576</identifier><identifier type="ArticleID">BF01875576</identifier><identifier type="ArticleID">Art8</identifier><accessCondition type="use and reproduction" contentType="copyright">Kugler Publication, 1996</accessCondition><recordInfo><recordContentSource>SPRINGER</recordContentSource><recordOrigin>Kugler Publication, 1996</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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