Sulphur, thiols, and disulphides in the fish epidermis, with remarks on keratinization
Identifieur interne : 000B60 ( Istex/Corpus ); précédent : 000B59; suivant : 000B61Sulphur, thiols, and disulphides in the fish epidermis, with remarks on keratinization
Auteurs : W. Meyer ; U. Seegers ; R. StelzerSource :
- Journal of Fish Biology [ 0022-1112 ] ; 2007-10.
English descriptors
- KwdEn :
Abstract
Energy dispersive x‐ray (EDX) analysis and qualitative and quantitative histochemistry were applied to study the distribution and contents of sulphur, thiols and disulphides in the epidermis of the river lamprey Lampetra fluviatilis, the lesser spotted dogfish Scyliorhinus canicula and the brown trout Salmo trutta fario. Thiols generally reacted weakly throughout the entire epidermis, whereas disulphide reactions were more distinct and differentiated. In the river lamprey, the concentrations of ‐S‐S‐ groups clearly increased in the developing mucous cells from the stratum basale to the stratum superficiale; skein cells and granular cells reacted negatively to weakly. In the lesser spotted dogfish, amounts of disulphides appeared at moderate concentrations, and only goblet cells displayed a strong reaction. In the brown trout, filament cells showed low concentrations or weak reactions of disulphides, goblet cells and the most outer superficial cells stained strongly. Sulphur distribution and contents generally supported the histochemical observations in normal epidermis cells (absolute sulphur contents: 41–59 mM), only the brown trout showed high amounts of sulphur in the stratum basale (81 mM). The findings corroborate the view that there is an inverse correlation between keratinization and mucous secretion in normal fish epidermis. The sometimes distinct contents of disulphides in the outer mucous layer indicate that this system could endure higher mechanical stress than predictable from its large amounts of neutral glycoproteins.
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DOI: 10.1111/j.1095-8649.2007.01585.x
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<front><div type="abstract" xml:lang="en">Energy dispersive x‐ray (EDX) analysis and qualitative and quantitative histochemistry were applied to study the distribution and contents of sulphur, thiols and disulphides in the epidermis of the river lamprey Lampetra fluviatilis, the lesser spotted dogfish Scyliorhinus canicula and the brown trout Salmo trutta fario. Thiols generally reacted weakly throughout the entire epidermis, whereas disulphide reactions were more distinct and differentiated. In the river lamprey, the concentrations of ‐S‐S‐ groups clearly increased in the developing mucous cells from the stratum basale to the stratum superficiale; skein cells and granular cells reacted negatively to weakly. In the lesser spotted dogfish, amounts of disulphides appeared at moderate concentrations, and only goblet cells displayed a strong reaction. In the brown trout, filament cells showed low concentrations or weak reactions of disulphides, goblet cells and the most outer superficial cells stained strongly. Sulphur distribution and contents generally supported the histochemical observations in normal epidermis cells (absolute sulphur contents: 41–59 mM), only the brown trout showed high amounts of sulphur in the stratum basale (81 mM). The findings corroborate the view that there is an inverse correlation between keratinization and mucous secretion in normal fish epidermis. The sometimes distinct contents of disulphides in the outer mucous layer indicate that this system could endure higher mechanical stress than predictable from its large amounts of neutral glycoproteins.</div>
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<abstractGroup><abstract type="main" xml:lang="en"><p>Energy dispersive x‐ray (EDX) analysis and qualitative and quantitative histochemistry were applied to study the distribution and contents of sulphur, thiols and disulphides in the epidermis of the river lamprey <i>Lampetra fluviatilis</i>
, the lesser spotted dogfish <i>Scyliorhinus canicula</i>
and the brown trout <i>Salmo trutta fario</i>
. Thiols generally reacted weakly throughout the entire epidermis, whereas disulphide reactions were more distinct and differentiated. In the river lamprey, the concentrations of ‐S‐S‐ groups clearly increased in the developing mucous cells from the stratum basale to the stratum superficiale; skein cells and granular cells reacted negatively to weakly. In the lesser spotted dogfish, amounts of disulphides appeared at moderate concentrations, and only goblet cells displayed a strong reaction. In the brown trout, filament cells showed low concentrations or weak reactions of disulphides, goblet cells and the most outer superficial cells stained strongly. Sulphur distribution and contents generally supported the histochemical observations in normal epidermis cells (absolute sulphur contents: 41–59 mM), only the brown trout showed high amounts of sulphur in the stratum basale (81 mM). The findings corroborate the view that there is an inverse correlation between keratinization and mucous secretion in normal fish epidermis. The sometimes distinct contents of disulphides in the outer mucous layer indicate that this system could endure higher mechanical stress than predictable from its large amounts of neutral glycoproteins.</p>
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<mods version="3.6"><titleInfo lang="en"><title>Sulphur, thiols, and disulphides in the fish epidermis, with remarks on keratinization</title>
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<titleInfo type="alternative" contentType="CDATA" lang="en"><title>Sulphur, thiols, and disulphides in the fish epidermis, with remarks on keratinization</title>
</titleInfo>
<name type="personal"><namePart type="given">W.</namePart>
<namePart type="family">Meyer</namePart>
<affiliation>* Institute of Anatomy, University of Veterinary Medicine Hannover, Bischofsholer Damm 15, 30173 Hannover, Germany , ‡Institute of Pharmacology, Toxicology and Pharmacy, University of Veterinary Medicine Hannover, Germany and §Institute of Animal Ecology and Cell Biology, University of Veterinary Medicine Hannover, Germany</affiliation>
<affiliation>E-mail: wilfried.meyer@tiho‐hannover.de</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">U.</namePart>
<namePart type="family">Seegers</namePart>
<affiliation>* Institute of Anatomy, University of Veterinary Medicine Hannover, Bischofsholer Damm 15, 30173 Hannover, Germany , ‡Institute of Pharmacology, Toxicology and Pharmacy, University of Veterinary Medicine Hannover, Germany and §Institute of Animal Ecology and Cell Biology, University of Veterinary Medicine Hannover, Germany</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">R.</namePart>
<namePart type="family">Stelzer</namePart>
<affiliation>* Institute of Anatomy, University of Veterinary Medicine Hannover, Bischofsholer Damm 15, 30173 Hannover, Germany , ‡Institute of Pharmacology, Toxicology and Pharmacy, University of Veterinary Medicine Hannover, Germany and §Institute of Animal Ecology and Cell Biology, University of Veterinary Medicine Hannover, Germany</affiliation>
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</role>
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<typeOfResource>text</typeOfResource>
<genre type="article" displayLabel="article"></genre>
<originInfo><publisher>Blackwell Publishing Ltd</publisher>
<place><placeTerm type="text">Oxford, UK</placeTerm>
</place>
<dateIssued encoding="w3cdtf">2007-10</dateIssued>
<edition>(Received 29 November 2004, Accepted 18 May 2007)</edition>
<copyrightDate encoding="w3cdtf">2007</copyrightDate>
</originInfo>
<language><languageTerm type="code" authority="rfc3066">en</languageTerm>
<languageTerm type="code" authority="iso639-2b">eng</languageTerm>
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<extent unit="figures">16</extent>
<extent unit="references">32</extent>
</physicalDescription>
<abstract lang="en">Energy dispersive x‐ray (EDX) analysis and qualitative and quantitative histochemistry were applied to study the distribution and contents of sulphur, thiols and disulphides in the epidermis of the river lamprey Lampetra fluviatilis, the lesser spotted dogfish Scyliorhinus canicula and the brown trout Salmo trutta fario. Thiols generally reacted weakly throughout the entire epidermis, whereas disulphide reactions were more distinct and differentiated. In the river lamprey, the concentrations of ‐S‐S‐ groups clearly increased in the developing mucous cells from the stratum basale to the stratum superficiale; skein cells and granular cells reacted negatively to weakly. In the lesser spotted dogfish, amounts of disulphides appeared at moderate concentrations, and only goblet cells displayed a strong reaction. In the brown trout, filament cells showed low concentrations or weak reactions of disulphides, goblet cells and the most outer superficial cells stained strongly. Sulphur distribution and contents generally supported the histochemical observations in normal epidermis cells (absolute sulphur contents: 41–59 mM), only the brown trout showed high amounts of sulphur in the stratum basale (81 mM). The findings corroborate the view that there is an inverse correlation between keratinization and mucous secretion in normal fish epidermis. The sometimes distinct contents of disulphides in the outer mucous layer indicate that this system could endure higher mechanical stress than predictable from its large amounts of neutral glycoproteins.</abstract>
<subject lang="en"><genre>keywords</genre>
<topic>disulphides</topic>
<topic>EDX analysis</topic>
<topic>fish epidermis</topic>
<topic>histochemistry</topic>
<topic>sulphur</topic>
<topic>thiols</topic>
</subject>
<relatedItem type="host"><titleInfo><title>Journal of Fish Biology</title>
</titleInfo>
<genre type="journal">journal</genre>
<identifier type="ISSN">0022-1112</identifier>
<identifier type="eISSN">1095-8649</identifier>
<identifier type="DOI">10.1111/(ISSN)1095-8649</identifier>
<identifier type="PublisherID">JFB</identifier>
<part><date>2007</date>
<detail type="volume"><caption>vol.</caption>
<number>71</number>
</detail>
<detail type="issue"><caption>no.</caption>
<number>4</number>
</detail>
<extent unit="pages"><start>1135</start>
<end>1144</end>
<total>10</total>
</extent>
</part>
</relatedItem>
<identifier type="istex">E18731258EB3F313F769544CD5AE0321965B3BE7</identifier>
<identifier type="DOI">10.1111/j.1095-8649.2007.01585.x</identifier>
<identifier type="ArticleID">JFB1585</identifier>
<accessCondition type="use and reproduction" contentType="copyright">2007 The Fisheries Society of the British Isles</accessCondition>
<recordInfo><recordContentSource>WILEY</recordContentSource>
<recordOrigin>Blackwell Publishing Ltd</recordOrigin>
</recordInfo>
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