Sulphur, thiols, and disulphides in the fish epidermis, with remarks on keratinization
Identifieur interne : 000B60 ( Istex/Corpus ); précédent : 000B59; suivant : 000B61Sulphur, thiols, and disulphides in the fish epidermis, with remarks on keratinization
Auteurs : W. Meyer ; U. Seegers ; R. StelzerSource :
- Journal of Fish Biology [ 0022-1112 ] ; 2007-10.
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- KwdEn :
Abstract
Energy dispersive x‐ray (EDX) analysis and qualitative and quantitative histochemistry were applied to study the distribution and contents of sulphur, thiols and disulphides in the epidermis of the river lamprey Lampetra fluviatilis, the lesser spotted dogfish Scyliorhinus canicula and the brown trout Salmo trutta fario. Thiols generally reacted weakly throughout the entire epidermis, whereas disulphide reactions were more distinct and differentiated. In the river lamprey, the concentrations of ‐S‐S‐ groups clearly increased in the developing mucous cells from the stratum basale to the stratum superficiale; skein cells and granular cells reacted negatively to weakly. In the lesser spotted dogfish, amounts of disulphides appeared at moderate concentrations, and only goblet cells displayed a strong reaction. In the brown trout, filament cells showed low concentrations or weak reactions of disulphides, goblet cells and the most outer superficial cells stained strongly. Sulphur distribution and contents generally supported the histochemical observations in normal epidermis cells (absolute sulphur contents: 41–59 mM), only the brown trout showed high amounts of sulphur in the stratum basale (81 mM). The findings corroborate the view that there is an inverse correlation between keratinization and mucous secretion in normal fish epidermis. The sometimes distinct contents of disulphides in the outer mucous layer indicate that this system could endure higher mechanical stress than predictable from its large amounts of neutral glycoproteins.
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DOI: 10.1111/j.1095-8649.2007.01585.x
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ISTEX:E18731258EB3F313F769544CD5AE0321965B3BE7Le document en format XML
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Meyer</name><affiliation><mods:affiliation>* Institute of Anatomy, University of Veterinary Medicine Hannover, Bischofsholer Damm 15, 30173 Hannover, Germany , ‡Institute of Pharmacology, Toxicology and Pharmacy, University of Veterinary Medicine Hannover, Germany and §Institute of Animal Ecology and Cell Biology, University of Veterinary Medicine Hannover, Germany</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: wilfried.meyer@tiho‐hannover.de</mods:affiliation></affiliation></author><author><name sortKey="Seegers, U" sort="Seegers, U" uniqKey="Seegers U" first="U." last="Seegers">U. 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Thiols generally reacted weakly throughout the entire epidermis, whereas disulphide reactions were more distinct and differentiated. In the river lamprey, the concentrations of ‐S‐S‐ groups clearly increased in the developing mucous cells from the stratum basale to the stratum superficiale; skein cells and granular cells reacted negatively to weakly. In the lesser spotted dogfish, amounts of disulphides appeared at moderate concentrations, and only goblet cells displayed a strong reaction. In the brown trout, filament cells showed low concentrations or weak reactions of disulphides, goblet cells and the most outer superficial cells stained strongly. Sulphur distribution and contents generally supported the histochemical observations in normal epidermis cells (absolute sulphur contents: 41–59 mM), only the brown trout showed high amounts of sulphur in the stratum basale (81 mM). The findings corroborate the view that there is an inverse correlation between keratinization and mucous secretion in normal fish epidermis. The sometimes distinct contents of disulphides in the outer mucous layer indicate that this system could endure higher mechanical stress than predictable from its large amounts of neutral glycoproteins.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>W. Meyer</name><affiliations><json:string>* Institute of Anatomy, University of Veterinary Medicine Hannover, Bischofsholer Damm 15, 30173 Hannover, Germany , ‡Institute of Pharmacology, Toxicology and Pharmacy, University of Veterinary Medicine Hannover, Germany and §Institute of Animal Ecology and Cell Biology, University of Veterinary Medicine Hannover, Germany</json:string><json:string>E-mail: wilfried.meyer@tiho‐hannover.de</json:string></affiliations></json:item><json:item><name>U. 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Thiols generally reacted weakly throughout the entire epidermis, whereas disulphide reactions were more distinct and differentiated. In the river lamprey, the concentrations of ‐S‐S‐ groups clearly increased in the developing mucous cells from the stratum basale to the stratum superficiale; skein cells and granular cells reacted negatively to weakly. In the lesser spotted dogfish, amounts of disulphides appeared at moderate concentrations, and only goblet cells displayed a strong reaction. In the brown trout, filament cells showed low concentrations or weak reactions of disulphides, goblet cells and the most outer superficial cells stained strongly. Sulphur distribution and contents generally supported the histochemical observations in normal epidermis cells (absolute sulphur contents: 41–59 mM), only the brown trout showed high amounts of sulphur in the stratum basale (81 mM). 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lamprey Lampetra fluviatilis, the lesser spotted dogfish Scyliorhinus canicula and the brown trout Salmo trutta fario. Thiols generally reacted weakly throughout the entire epidermis, whereas disulphide reactions were more distinct and differentiated. In the river lamprey, the concentrations of ‐S‐S‐ groups clearly increased in the developing mucous cells from the stratum basale to the stratum superficiale; skein cells and granular cells reacted negatively to weakly. In the lesser spotted dogfish, amounts of disulphides appeared at moderate concentrations, and only goblet cells displayed a strong reaction. In the brown trout, filament cells showed low concentrations or weak reactions of disulphides, goblet cells and the most outer superficial cells stained strongly. Sulphur distribution and contents generally supported the histochemical observations in normal epidermis cells (absolute sulphur contents: 41–59 mM), only the brown trout showed high amounts of sulphur in the stratum basale (81 mM). The findings corroborate the view that there is an inverse correlation between keratinization and mucous secretion in normal fish epidermis. The sometimes distinct contents of disulphides in the outer mucous layer indicate that this system could endure higher mechanical stress than predictable from its large amounts of neutral glycoproteins.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>keywords</head><item><term>disulphides</term></item><item><term>EDX analysis</term></item><item><term>fish epidermis</term></item><item><term>histochemistry</term></item><item><term>sulphur</term></item><item><term>thiols</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2007-10">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/E18731258EB3F313F769544CD5AE0321965B3BE7/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>Blackwell Publishing Ltd</publisherName><publisherLoc>Oxford, UK</publisherLoc></publisherInfo><doi origin="wiley" registered="yes">10.1111/(ISSN)1095-8649</doi><issn type="print">0022-1112</issn><issn type="electronic">1095-8649</issn><idGroup><id type="product" value="JFB"></id><id type="publisherDivision" value="ST"></id></idGroup><titleGroup><title type="main" sort="JOURNAL OF FISH BIOLOGY">Journal of Fish Biology</title></titleGroup></publicationMeta><publicationMeta level="part" position="10004"><doi origin="wiley">10.1111/jfb.2007.71.issue-4</doi><numberingGroup><numbering type="journalVolume" number="71">71</numbering><numbering type="journalIssue" number="4">4</numbering></numberingGroup><coverDate startDate="2007-10">October 2007</coverDate></publicationMeta><publicationMeta level="unit" type="article" position="13" status="forIssue"><doi origin="wiley">10.1111/j.1095-8649.2007.01585.x</doi><idGroup><id type="unit" value="JFB1585"></id></idGroup><countGroup><count type="pageTotal" number="10"></count></countGroup><titleGroup><title type="tocHeading1">REGULAR PAPERS</title></titleGroup><copyright>2007 The Fisheries Society of the British Isles</copyright><eventGroup><event type="firstOnline" date="2007-10-09"></event><event type="publishedOnlineFinalForm" date="2007-10-09"></event><event type="xmlConverted" agent="Converter:BPG_TO_WML3G version:2.3.5 mode:FullText source:HeaderRef result:HeaderRef" date="2010-04-06"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:3.8.8" date="2014-01-30"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-10-30"></event></eventGroup><numberingGroup><numbering type="pageFirst" number="1135">1135</numbering><numbering type="pageLast" number="1144">1144</numbering></numberingGroup><correspondenceTo> †Tel.: +49 511 8567215; fax: +49 511 8567683; email: <email normalForm="wilfried.meyer@tiho-hannover.de">wilfried.meyer@tiho‐hannover.de</email></correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:JFB.JFB1585.pdf"></link></linkGroup></publicationMeta><contentMeta><unparsedEditorialHistory>(Received 29 November 2004, Accepted 18 May 2007)</unparsedEditorialHistory><countGroup><count type="figureTotal" number="16"></count><count type="tableTotal" number="0"></count><count type="formulaTotal" number="0"></count><count type="referenceTotal" number="32"></count><count type="wordTotal" number="0"></count><count type="linksPubMed" number="0"></count><count type="linksCrossRef" number="0"></count></countGroup><titleGroup><title type="main">Sulphur, thiols, and disulphides in the fish epidermis, with remarks on keratinization</title></titleGroup><creators><creator creatorRole="author" xml:id="cr1" affiliationRef="#aff-1-1" corresponding="yes"><personName><givenNames>W.</givenNames><familyName>Meyer</familyName></personName></creator><creator creatorRole="author" xml:id="cr2" affiliationRef="#aff-1-1"><personName><givenNames>U.</givenNames><familyName>Seegers</familyName></personName></creator><creator creatorRole="author" xml:id="cr3" affiliationRef="#aff-1-1"><personName><givenNames>R.</givenNames><familyName>Stelzer</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="aff-1-1" countryCode="DE"><unparsedAffiliation> * <i>Institute of Anatomy, University of Veterinary Medicine Hannover, Bischofsholer Damm 15, 30173 Hannover, Germany</i> , ‡<i>Institute of Pharmacology, Toxicology and Pharmacy, University of Veterinary Medicine Hannover, Germany</i><i> and </i>§<i>Institute of Animal Ecology and Cell Biology, University of Veterinary Medicine Hannover, Germany</i></unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en"><keyword xml:id="k1">disulphides</keyword><keyword xml:id="k2">EDX analysis</keyword><keyword xml:id="k3">fish epidermis</keyword><keyword xml:id="k4">histochemistry</keyword><keyword xml:id="k5">sulphur</keyword><keyword xml:id="k6">thiols</keyword></keywordGroup><abstractGroup><abstract type="main" xml:lang="en"><p>Energy dispersive x‐ray (EDX) analysis and qualitative and quantitative histochemistry were applied to study the distribution and contents of sulphur, thiols and disulphides in the epidermis of the river lamprey <i>Lampetra fluviatilis</i>, the lesser spotted dogfish <i>Scyliorhinus canicula</i> and the brown trout <i>Salmo trutta fario</i>. Thiols generally reacted weakly throughout the entire epidermis, whereas disulphide reactions were more distinct and differentiated. In the river lamprey, the concentrations of ‐S‐S‐ groups clearly increased in the developing mucous cells from the stratum basale to the stratum superficiale; skein cells and granular cells reacted negatively to weakly. In the lesser spotted dogfish, amounts of disulphides appeared at moderate concentrations, and only goblet cells displayed a strong reaction. In the brown trout, filament cells showed low concentrations or weak reactions of disulphides, goblet cells and the most outer superficial cells stained strongly. Sulphur distribution and contents generally supported the histochemical observations in normal epidermis cells (absolute sulphur contents: 41–59 mM), only the brown trout showed high amounts of sulphur in the stratum basale (81 mM). The findings corroborate the view that there is an inverse correlation between keratinization and mucous secretion in normal fish epidermis. The sometimes distinct contents of disulphides in the outer mucous layer indicate that this system could endure higher mechanical stress than predictable from its large amounts of neutral glycoproteins.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Sulphur, thiols, and disulphides in the fish epidermis, with remarks on keratinization</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Sulphur, thiols, and disulphides in the fish epidermis, with remarks on keratinization</title></titleInfo><name type="personal"><namePart type="given">W.</namePart><namePart type="family">Meyer</namePart><affiliation>* Institute of Anatomy, University of Veterinary Medicine Hannover, Bischofsholer Damm 15, 30173 Hannover, Germany , ‡Institute of Pharmacology, Toxicology and Pharmacy, University of Veterinary Medicine Hannover, Germany and §Institute of Animal Ecology and Cell Biology, University of Veterinary Medicine Hannover, Germany</affiliation><affiliation>E-mail: wilfried.meyer@tiho‐hannover.de</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">U.</namePart><namePart type="family">Seegers</namePart><affiliation>* Institute of Anatomy, University of Veterinary Medicine Hannover, Bischofsholer Damm 15, 30173 Hannover, Germany , ‡Institute of Pharmacology, Toxicology and Pharmacy, University of Veterinary Medicine Hannover, Germany and §Institute of Animal Ecology and Cell Biology, University of Veterinary Medicine Hannover, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">R.</namePart><namePart type="family">Stelzer</namePart><affiliation>* Institute of Anatomy, University of Veterinary Medicine Hannover, Bischofsholer Damm 15, 30173 Hannover, Germany , ‡Institute of Pharmacology, Toxicology and Pharmacy, University of Veterinary Medicine Hannover, Germany and §Institute of Animal Ecology and Cell Biology, University of Veterinary Medicine Hannover, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Blackwell Publishing Ltd</publisher><place><placeTerm type="text">Oxford, UK</placeTerm></place><dateIssued encoding="w3cdtf">2007-10</dateIssued><edition>(Received 29 November 2004, Accepted 18 May 2007)</edition><copyrightDate encoding="w3cdtf">2007</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">16</extent><extent unit="references">32</extent></physicalDescription><abstract lang="en">Energy dispersive x‐ray (EDX) analysis and qualitative and quantitative histochemistry were applied to study the distribution and contents of sulphur, thiols and disulphides in the epidermis of the river lamprey Lampetra fluviatilis, the lesser spotted dogfish Scyliorhinus canicula and the brown trout Salmo trutta fario. Thiols generally reacted weakly throughout the entire epidermis, whereas disulphide reactions were more distinct and differentiated. In the river lamprey, the concentrations of ‐S‐S‐ groups clearly increased in the developing mucous cells from the stratum basale to the stratum superficiale; skein cells and granular cells reacted negatively to weakly. In the lesser spotted dogfish, amounts of disulphides appeared at moderate concentrations, and only goblet cells displayed a strong reaction. In the brown trout, filament cells showed low concentrations or weak reactions of disulphides, goblet cells and the most outer superficial cells stained strongly. Sulphur distribution and contents generally supported the histochemical observations in normal epidermis cells (absolute sulphur contents: 41–59 mM), only the brown trout showed high amounts of sulphur in the stratum basale (81 mM). The findings corroborate the view that there is an inverse correlation between keratinization and mucous secretion in normal fish epidermis. The sometimes distinct contents of disulphides in the outer mucous layer indicate that this system could endure higher mechanical stress than predictable from its large amounts of neutral glycoproteins.</abstract><subject lang="en"><genre>keywords</genre><topic>disulphides</topic><topic>EDX analysis</topic><topic>fish epidermis</topic><topic>histochemistry</topic><topic>sulphur</topic><topic>thiols</topic></subject><relatedItem type="host"><titleInfo><title>Journal of Fish Biology</title></titleInfo><genre type="journal">journal</genre><identifier type="ISSN">0022-1112</identifier><identifier type="eISSN">1095-8649</identifier><identifier type="DOI">10.1111/(ISSN)1095-8649</identifier><identifier type="PublisherID">JFB</identifier><part><date>2007</date><detail type="volume"><caption>vol.</caption><number>71</number></detail><detail type="issue"><caption>no.</caption><number>4</number></detail><extent unit="pages"><start>1135</start><end>1144</end><total>10</total></extent></part></relatedItem><identifier type="istex">E18731258EB3F313F769544CD5AE0321965B3BE7</identifier><identifier type="DOI">10.1111/j.1095-8649.2007.01585.x</identifier><identifier type="ArticleID">JFB1585</identifier><accessCondition type="use and reproduction" contentType="copyright">2007 The Fisheries Society of the British Isles</accessCondition><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>Blackwell Publishing Ltd</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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