Glutathione-dependent resistance of the European eel Anguilla anguilla to the herbicide molinate
Identifieur interne : 000B39 ( Istex/Corpus ); précédent : 000B38; suivant : 000B40Glutathione-dependent resistance of the European eel Anguilla anguilla to the herbicide molinate
Auteurs : S. Pe A-Llopis ; J. B. Pe A ; E. Sancho ; C. Fernández-Vega ; M. D. FerrandoSource :
- Chemosphere [ 0045-6535 ] ; 2001.
Abstract
Eels of species Anguilla anguilla were exposed to 5/4 LC50 (41.8 mg/l) of the herbicide molinate for 96 h in a time to death (TTD) test. Glutathione content (GSx, GSH, GSSG), glutathione reductase (GR) and γ-glutamyl transpeptidase (γ-GT) activities were determined in the liver and muscle tissues of dead and surviving (intoxicated) animals and compared to control values (non-exposed eels). TTD was positively correlated to hepatic GSH, GSH:GSSG ratio, hepatic and muscular GR, but negatively correlated to muscular GSH, which was severely depleted. Furthermore, glutathione and enzyme activities were intercorrelated, especially GSH and GR. These results indicate that eels which were able to induce GR activity, increase GSH and maintain the GSH:GSSG ratio in the liver showed an extended survival under the oxidative stress generated by molinate than those that lost glutathione homeostasis.
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DOI: 10.1016/S0045-6535(00)00500-2
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Sancho</name><affiliation><mods:affiliation>Department of Animal Biology (Animal Physiology), Faculty of Biology, University of Valencia, Dr. Moliner-50, E-46100 Burjassot, Valencia, Spain</mods:affiliation></affiliation></author><author><name sortKey="Fernandez Vega, C" sort="Fernandez Vega, C" uniqKey="Fernandez Vega C" first="C." last="Fernández-Vega">C. Fernández-Vega</name><affiliation><mods:affiliation>Department of Animal Biology (Animal Physiology), Faculty of Biology, University of Valencia, Dr. Moliner-50, E-46100 Burjassot, Valencia, Spain</mods:affiliation></affiliation></author><author><name sortKey="Ferrando, M D" sort="Ferrando, M D" uniqKey="Ferrando M" first="M. D." last="Ferrando">M. D. Ferrando</name><affiliation><mods:affiliation>Department of Animal Biology (Animal Physiology), Faculty of Biology, University of Valencia, Dr. Moliner-50, E-46100 Burjassot, Valencia, Spain</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:6387D9C30C292CF17368841B99B181DC42BADC72</idno><date when="2001" year="2001">2001</date><idno type="doi">10.1016/S0045-6535(00)00500-2</idno><idno type="url">https://api.istex.fr/document/6387D9C30C292CF17368841B99B181DC42BADC72/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000B39</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000B39</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Glutathione-dependent resistance of the European eel Anguilla anguilla to the herbicide molinate</title><author><name sortKey="Pe A Llopis, S" sort="Pe A Llopis, S" uniqKey="Pe A Llopis S" first="S." last="Pe A-Llopis">S. Pe A-Llopis</name><affiliation><mods:affiliation>E-mail: samuel@iats.csic.es</mods:affiliation></affiliation><affiliation><mods:affiliation>Institute of Aquaculture Torre de la Sal (CSIC), E-12595 Ribera de Cabanes, Castellón, Spain</mods:affiliation></affiliation></author><author><name sortKey="Pe A, J B" sort="Pe A, J B" uniqKey="Pe A J" first="J. B." last="Pe A">J. B. Pe A</name><affiliation><mods:affiliation>Institute of Aquaculture Torre de la Sal (CSIC), E-12595 Ribera de Cabanes, Castellón, Spain</mods:affiliation></affiliation></author><author><name sortKey="Sancho, E" sort="Sancho, E" uniqKey="Sancho E" first="E." last="Sancho">E. Sancho</name><affiliation><mods:affiliation>Department of Animal Biology (Animal Physiology), Faculty of Biology, University of Valencia, Dr. Moliner-50, E-46100 Burjassot, Valencia, Spain</mods:affiliation></affiliation></author><author><name sortKey="Fernandez Vega, C" sort="Fernandez Vega, C" uniqKey="Fernandez Vega C" first="C." last="Fernández-Vega">C. Fernández-Vega</name><affiliation><mods:affiliation>Department of Animal Biology (Animal Physiology), Faculty of Biology, University of Valencia, Dr. Moliner-50, E-46100 Burjassot, Valencia, Spain</mods:affiliation></affiliation></author><author><name sortKey="Ferrando, M D" sort="Ferrando, M D" uniqKey="Ferrando M" first="M. D." last="Ferrando">M. D. Ferrando</name><affiliation><mods:affiliation>Department of Animal Biology (Animal Physiology), Faculty of Biology, University of Valencia, Dr. Moliner-50, E-46100 Burjassot, Valencia, Spain</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Chemosphere</title><title level="j" type="abbrev">CHEM</title><idno type="ISSN">0045-6535</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="2001">2001</date><biblScope unit="volume">45</biblScope><biblScope unit="issue">4–5</biblScope><biblScope unit="page" from="671">671</biblScope><biblScope unit="page" to="681">681</biblScope></imprint><idno type="ISSN">0045-6535</idno></series><idno type="istex">6387D9C30C292CF17368841B99B181DC42BADC72</idno><idno type="DOI">10.1016/S0045-6535(00)00500-2</idno><idno type="PII">S0045-6535(00)00500-2</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0045-6535</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Eels of species Anguilla anguilla were exposed to 5/4 LC50 (41.8 mg/l) of the herbicide molinate for 96 h in a time to death (TTD) test. Glutathione content (GSx, GSH, GSSG), glutathione reductase (GR) and γ-glutamyl transpeptidase (γ-GT) activities were determined in the liver and muscle tissues of dead and surviving (intoxicated) animals and compared to control values (non-exposed eels). TTD was positively correlated to hepatic GSH, GSH:GSSG ratio, hepatic and muscular GR, but negatively correlated to muscular GSH, which was severely depleted. Furthermore, glutathione and enzyme activities were intercorrelated, especially GSH and GR. These results indicate that eels which were able to induce GR activity, increase GSH and maintain the GSH:GSSG ratio in the liver showed an extended survival under the oxidative stress generated by molinate than those that lost glutathione homeostasis.</div></front></TEI><istex><corpusName>elsevier</corpusName><author><json:item><name>S. Peña-Llopis</name><affiliations><json:string>E-mail: samuel@iats.csic.es</json:string><json:string>Institute of Aquaculture Torre de la Sal (CSIC), E-12595 Ribera de Cabanes, Castellón, Spain</json:string></affiliations></json:item><json:item><name>J.B. Peña</name><affiliations><json:string>Institute of Aquaculture Torre de la Sal (CSIC), E-12595 Ribera de Cabanes, Castellón, Spain</json:string></affiliations></json:item><json:item><name>E. Sancho</name><affiliations><json:string>Department of Animal Biology (Animal Physiology), Faculty of Biology, University of Valencia, Dr. Moliner-50, E-46100 Burjassot, Valencia, Spain</json:string></affiliations></json:item><json:item><name>C. Fernández-Vega</name><affiliations><json:string>Department of Animal Biology (Animal Physiology), Faculty of Biology, University of Valencia, Dr. Moliner-50, E-46100 Burjassot, Valencia, Spain</json:string></affiliations></json:item><json:item><name>M.D. Ferrando</name><affiliations><json:string>Department of Animal Biology (Animal Physiology), Faculty of Biology, University of Valencia, Dr. Moliner-50, E-46100 Burjassot, Valencia, Spain</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Pesticide</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Oxidative stress</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Fish</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Reductase</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Transpeptidase</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>TTD</value></json:item></subject><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>Eels of species Anguilla anguilla were exposed to 5/4 LC50 (41.8 mg/l) of the herbicide molinate for 96 h in a time to death (TTD) test. Glutathione content (GSx, GSH, GSSG), glutathione reductase (GR) and γ-glutamyl transpeptidase (γ-GT) activities were determined in the liver and muscle tissues of dead and surviving (intoxicated) animals and compared to control values (non-exposed eels). TTD was positively correlated to hepatic GSH, GSH:GSSG ratio, hepatic and muscular GR, but negatively correlated to muscular GSH, which was severely depleted. Furthermore, glutathione and enzyme activities were intercorrelated, especially GSH and GR. These results indicate that eels which were able to induce GR activity, increase GSH and maintain the GSH:GSSG ratio in the liver showed an extended survival under the oxidative stress generated by molinate than those that lost glutathione homeostasis.</abstract><qualityIndicators><score>6.596</score><pdfVersion>1.2</pdfVersion><pdfPageSize>544 x 743 pts</pdfPageSize><refBibsNative>true</refBibsNative><keywordCount>6</keywordCount><abstractCharCount>896</abstractCharCount><pdfWordCount>5427</pdfWordCount><pdfCharCount>35074</pdfCharCount><pdfPageCount>11</pdfPageCount><abstractWordCount>133</abstractWordCount></qualityIndicators><title>Glutathione-dependent resistance of the European eel Anguilla anguilla to the herbicide molinate</title><pii><json:string>S0045-6535(00)00500-2</json:string></pii><genre><json:string>research-article</json:string></genre><host><volume>45</volume><pii><json:string>S0045-6535(00)X0166-X</json:string></pii><pages><last>681</last><first>671</first></pages><issn><json:string>0045-6535</json:string></issn><issue>4–5</issue><genre><json:string>journal</json:string></genre><language><json:string>unknown</json:string></language><title>Chemosphere</title><publicationDate>2001</publicationDate></host><categories><wos><json:string>science</json:string><json:string>environmental sciences</json:string></wos><scienceMetrix><json:string>natural sciences</json:string><json:string>earth & environmental sciences</json:string><json:string>environmental sciences</json:string></scienceMetrix></categories><publicationDate>2001</publicationDate><copyrightDate>2001</copyrightDate><doi><json:string>10.1016/S0045-6535(00)00500-2</json:string></doi><id>6387D9C30C292CF17368841B99B181DC42BADC72</id><score>0.03567499</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/document/6387D9C30C292CF17368841B99B181DC42BADC72/fulltext/pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/document/6387D9C30C292CF17368841B99B181DC42BADC72/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/6387D9C30C292CF17368841B99B181DC42BADC72/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a">Glutathione-dependent resistance of the European eel Anguilla anguilla to the herbicide molinate</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>ELSEVIER</publisher><availability><p>©2001 Elsevier Science Ltd</p></availability><date>2001</date></publicationStmt><notesStmt><note type="content">Fig. 1: Total (GSx), reduced (GSH) and oxidized (GSSG) glutathione in the liver (a) and muscle (b) of European eels exposed to 41.8 mg/l of molinate. Eels were grouped in four intervals of time according to their death time until 96 h, where surviving eels were considered as another group. Values are mean±SEM. (*) P<0.05; (**) P<0.01.</note><note type="content">Fig. 2: Relationship between reduced (GSH) and oxidized (GSSG) glutathione in the liver (a) and muscle (b) of eels exposed to 41.8 mg/l of molinate. Eels were grouped in four intervals of time according to their death time until 96 h, and survivors were considered as another group. Values are mean±SEM. (*) P<0.05; (**) P<0.01.</note><note type="content">Fig. 3: Glutathione reductase activity (GR) in both liver and muscle tissues of controls and eels exposed to 41.8 mg/l of molinate. Values are means±SEM. (*) P<0.05; (**) P<0.01.</note><note type="content">Fig. 4: γ-Glutamyl transpeptidase activity (γ-GT) in both liver and muscle tissues of controls and eels exposed to 41.8 mg/l of molinate. Values are mean±SEM. (*) P<0.05. p-NA=p-nitroanilide.</note><note type="content">Table 1: Pearson correlation coefficients (r) among TTD, glutathione and activities of glutathione-dependent enzymes in the liver and muscle from individual eels exposed to molinatea</note><note type="content">Table 2: ANOVA results of glutathione levels and activities of glutathione-dependent enzymes in the liver and muscle from eels exposed to molinate and control ones</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">Glutathione-dependent resistance of the European eel Anguilla anguilla to the herbicide molinate</title><author xml:id="author-1"><persName><forename type="first">S.</forename><surname>Peña-Llopis</surname></persName><email>samuel@iats.csic.es</email><affiliation>Institute of Aquaculture Torre de la Sal (CSIC), E-12595 Ribera de Cabanes, Castellón, Spain</affiliation></author><author xml:id="author-2"><persName><forename type="first">J.B.</forename><surname>Peña</surname></persName><affiliation>Institute of Aquaculture Torre de la Sal (CSIC), E-12595 Ribera de Cabanes, Castellón, Spain</affiliation></author><author xml:id="author-3"><persName><forename type="first">E.</forename><surname>Sancho</surname></persName><affiliation>Department of Animal Biology (Animal Physiology), Faculty of Biology, University of Valencia, Dr. Moliner-50, E-46100 Burjassot, Valencia, Spain</affiliation></author><author xml:id="author-4"><persName><forename type="first">C.</forename><surname>Fernández-Vega</surname></persName><affiliation>Department of Animal Biology (Animal Physiology), Faculty of Biology, University of Valencia, Dr. Moliner-50, E-46100 Burjassot, Valencia, Spain</affiliation></author><author xml:id="author-5"><persName><forename type="first">M.D.</forename><surname>Ferrando</surname></persName><note type="correspondence"><p>Corresponding author. Tel.: +34-63864676; fax: +36-963864372</p></note><affiliation>Department of Animal Biology (Animal Physiology), Faculty of Biology, University of Valencia, Dr. Moliner-50, E-46100 Burjassot, Valencia, Spain</affiliation></author></analytic><monogr><title level="j">Chemosphere</title><title level="j" type="abbrev">CHEM</title><idno type="pISSN">0045-6535</idno><idno type="PII">S0045-6535(00)X0166-X</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="2001"></date><biblScope unit="volume">45</biblScope><biblScope unit="issue">4–5</biblScope><biblScope unit="page" from="671">671</biblScope><biblScope unit="page" to="681">681</biblScope></imprint></monogr><idno type="istex">6387D9C30C292CF17368841B99B181DC42BADC72</idno><idno type="DOI">10.1016/S0045-6535(00)00500-2</idno><idno type="PII">S0045-6535(00)00500-2</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2001</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Eels of species Anguilla anguilla were exposed to 5/4 LC50 (41.8 mg/l) of the herbicide molinate for 96 h in a time to death (TTD) test. Glutathione content (GSx, GSH, GSSG), glutathione reductase (GR) and γ-glutamyl transpeptidase (γ-GT) activities were determined in the liver and muscle tissues of dead and surviving (intoxicated) animals and compared to control values (non-exposed eels). TTD was positively correlated to hepatic GSH, GSH:GSSG ratio, hepatic and muscular GR, but negatively correlated to muscular GSH, which was severely depleted. Furthermore, glutathione and enzyme activities were intercorrelated, especially GSH and GR. These results indicate that eels which were able to induce GR activity, increase GSH and maintain the GSH:GSSG ratio in the liver showed an extended survival under the oxidative stress generated by molinate than those that lost glutathione homeostasis.</p></abstract><textClass><keywords scheme="keyword"><list><head>Keywords</head><item><term>Pesticide</term></item><item><term>Oxidative stress</term></item><item><term>Fish</term></item><item><term>Reductase</term></item><item><term>Transpeptidase</term></item><item><term>TTD</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2001">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/6387D9C30C292CF17368841B99B181DC42BADC72/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: ce:floats; body; tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"><istex:entity SYSTEM="gr1" NDATA="IMAGE" name="gr1"></istex:entity><istex:entity SYSTEM="gr2" NDATA="IMAGE" name="gr2"></istex:entity><istex:entity SYSTEM="gr3" NDATA="IMAGE" name="gr3"></istex:entity><istex:entity SYSTEM="gr4" NDATA="IMAGE" name="gr4"></istex:entity></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>CHEM</jid><aid>3609</aid><ce:pii>S0045-6535(00)00500-2</ce:pii><ce:doi>10.1016/S0045-6535(00)00500-2</ce:doi><ce:copyright type="full-transfer" year="2001">Elsevier Science Ltd</ce:copyright></item-info><head><ce:title>Glutathione-dependent resistance of the European eel <ce:italic>Anguilla anguilla</ce:italic> to the herbicide molinate</ce:title><ce:author-group><ce:author biographyid="VT1"><ce:given-name>S.</ce:given-name><ce:surname>Peña-Llopis</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>a</ce:sup></ce:cross-ref><ce:e-address>samuel@iats.csic.es</ce:e-address></ce:author><ce:author biographyid="VT2"><ce:given-name>J.B.</ce:given-name><ce:surname>Peña</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>a</ce:sup></ce:cross-ref></ce:author><ce:author biographyid="VT3"><ce:given-name>E.</ce:given-name><ce:surname>Sancho</ce:surname><ce:cross-ref refid="AFF2"><ce:sup>b</ce:sup></ce:cross-ref></ce:author><ce:author biographyid="VT4"><ce:given-name>C.</ce:given-name><ce:surname>Fernández-Vega</ce:surname><ce:cross-ref refid="AFF2"><ce:sup>b</ce:sup></ce:cross-ref></ce:author><ce:author biographyid="VT5"><ce:given-name>M.D.</ce:given-name><ce:surname>Ferrando</ce:surname><ce:cross-ref refid="AFF2"><ce:sup>b</ce:sup></ce:cross-ref><ce:cross-ref refid="CORR1">*</ce:cross-ref></ce:author><ce:affiliation id="AFF1"><ce:label>a</ce:label><ce:textfn>Institute of Aquaculture Torre de la Sal (CSIC), E-12595 Ribera de Cabanes, Castellón, Spain</ce:textfn></ce:affiliation><ce:affiliation id="AFF2"><ce:label>b</ce:label><ce:textfn>Department of Animal Biology (Animal Physiology), Faculty of Biology, University of Valencia, Dr. Moliner-50, E-46100 Burjassot, Valencia, Spain</ce:textfn></ce:affiliation><ce:correspondence id="CORR1"><ce:label>*</ce:label><ce:text>Corresponding author. Tel.: +34-63864676; fax: +36-963864372</ce:text></ce:correspondence></ce:author-group><ce:date-received day="17" month="3" year="2000"></ce:date-received><ce:date-accepted day="1" month="8" year="2000"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>Eels of species <ce:italic>Anguilla anguilla</ce:italic> were exposed to 5/4 LC<ce:inf>50</ce:inf> (41.8 mg/l) of the herbicide molinate for 96 h in a time to death (TTD) test. Glutathione content (GSx, GSH, GSSG), glutathione reductase (GR) and γ-glutamyl transpeptidase (γ-GT) activities were determined in the liver and muscle tissues of dead and surviving (intoxicated) animals and compared to control values (non-exposed eels). TTD was positively correlated to hepatic GSH, GSH:GSSG ratio, hepatic and muscular GR, but negatively correlated to muscular GSH, which was severely depleted. Furthermore, glutathione and enzyme activities were intercorrelated, especially GSH and GR. These results indicate that eels which were able to induce GR activity, increase GSH and maintain the GSH:GSSG ratio in the liver showed an extended survival under the oxidative stress generated by molinate than those that lost glutathione homeostasis.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords class="keyword"><ce:section-title>Keywords</ce:section-title><ce:keyword><ce:text>Pesticide</ce:text></ce:keyword><ce:keyword><ce:text>Oxidative stress</ce:text></ce:keyword><ce:keyword><ce:text>Fish</ce:text></ce:keyword><ce:keyword><ce:text>Reductase</ce:text></ce:keyword><ce:keyword><ce:text>Transpeptidase</ce:text></ce:keyword><ce:keyword><ce:text>TTD</ce:text></ce:keyword></ce:keywords></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Glutathione-dependent resistance of the European eel Anguilla anguilla to the herbicide molinate</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Glutathione-dependent resistance of the European eel</title></titleInfo><name type="personal"><namePart type="given">S.</namePart><namePart type="family">Peña-Llopis</namePart><affiliation>E-mail: samuel@iats.csic.es</affiliation><affiliation>Institute of Aquaculture Torre de la Sal (CSIC), E-12595 Ribera de Cabanes, Castellón, Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J.B.</namePart><namePart type="family">Peña</namePart><affiliation>Institute of Aquaculture Torre de la Sal (CSIC), E-12595 Ribera de Cabanes, Castellón, Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">E.</namePart><namePart type="family">Sancho</namePart><affiliation>Department of Animal Biology (Animal Physiology), Faculty of Biology, University of Valencia, Dr. Moliner-50, E-46100 Burjassot, Valencia, Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">C.</namePart><namePart type="family">Fernández-Vega</namePart><affiliation>Department of Animal Biology (Animal Physiology), Faculty of Biology, University of Valencia, Dr. Moliner-50, E-46100 Burjassot, Valencia, Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.D.</namePart><namePart type="family">Ferrando</namePart><affiliation>Department of Animal Biology (Animal Physiology), Faculty of Biology, University of Valencia, Dr. Moliner-50, E-46100 Burjassot, Valencia, Spain</affiliation><description>Corresponding author. Tel.: +34-63864676; fax: +36-963864372</description><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article"></genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">2001</dateIssued><copyrightDate encoding="w3cdtf">2001</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">Eels of species Anguilla anguilla were exposed to 5/4 LC50 (41.8 mg/l) of the herbicide molinate for 96 h in a time to death (TTD) test. Glutathione content (GSx, GSH, GSSG), glutathione reductase (GR) and γ-glutamyl transpeptidase (γ-GT) activities were determined in the liver and muscle tissues of dead and surviving (intoxicated) animals and compared to control values (non-exposed eels). TTD was positively correlated to hepatic GSH, GSH:GSSG ratio, hepatic and muscular GR, but negatively correlated to muscular GSH, which was severely depleted. Furthermore, glutathione and enzyme activities were intercorrelated, especially GSH and GR. These results indicate that eels which were able to induce GR activity, increase GSH and maintain the GSH:GSSG ratio in the liver showed an extended survival under the oxidative stress generated by molinate than those that lost glutathione homeostasis.</abstract><note type="content">Fig. 1: Total (GSx), reduced (GSH) and oxidized (GSSG) glutathione in the liver (a) and muscle (b) of European eels exposed to 41.8 mg/l of molinate. Eels were grouped in four intervals of time according to their death time until 96 h, where surviving eels were considered as another group. Values are mean±SEM. (*) P<0.05; (**) P<0.01.</note><note type="content">Fig. 2: Relationship between reduced (GSH) and oxidized (GSSG) glutathione in the liver (a) and muscle (b) of eels exposed to 41.8 mg/l of molinate. Eels were grouped in four intervals of time according to their death time until 96 h, and survivors were considered as another group. Values are mean±SEM. (*) P<0.05; (**) P<0.01.</note><note type="content">Fig. 3: Glutathione reductase activity (GR) in both liver and muscle tissues of controls and eels exposed to 41.8 mg/l of molinate. Values are means±SEM. (*) P<0.05; (**) P<0.01.</note><note type="content">Fig. 4: γ-Glutamyl transpeptidase activity (γ-GT) in both liver and muscle tissues of controls and eels exposed to 41.8 mg/l of molinate. Values are mean±SEM. (*) P<0.05. p-NA=p-nitroanilide.</note><note type="content">Table 1: Pearson correlation coefficients (r) among TTD, glutathione and activities of glutathione-dependent enzymes in the liver and muscle from individual eels exposed to molinatea</note><note type="content">Table 2: ANOVA results of glutathione levels and activities of glutathione-dependent enzymes in the liver and muscle from eels exposed to molinate and control ones</note><subject><genre>Keywords</genre><topic>Pesticide</topic><topic>Oxidative stress</topic><topic>Fish</topic><topic>Reductase</topic><topic>Transpeptidase</topic><topic>TTD</topic></subject><relatedItem type="host"><titleInfo><title>Chemosphere</title></titleInfo><titleInfo type="abbreviated"><title>CHEM</title></titleInfo><genre type="journal">journal</genre><originInfo><dateIssued encoding="w3cdtf">200111</dateIssued></originInfo><identifier type="ISSN">0045-6535</identifier><identifier type="PII">S0045-6535(00)X0166-X</identifier><part><date>200111</date><detail type="volume"><number>45</number><caption>vol.</caption></detail><detail type="issue"><number>4–5</number><caption>no.</caption></detail><extent unit="issue pages"><start>399</start><end>704</end></extent><extent unit="pages"><start>671</start><end>681</end></extent></part></relatedItem><identifier type="istex">6387D9C30C292CF17368841B99B181DC42BADC72</identifier><identifier type="DOI">10.1016/S0045-6535(00)00500-2</identifier><identifier type="PII">S0045-6535(00)00500-2</identifier><accessCondition type="use and reproduction" contentType="copyright">©2001 Elsevier Science Ltd</accessCondition><recordInfo><recordContentSource>ELSEVIER</recordContentSource><recordOrigin>Elsevier Science Ltd, ©2001</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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