Effect of storage time on haematological parameters in mullet, Mugil cephalus
Identifieur interne : 000B30 ( Istex/Corpus ); précédent : 000B29; suivant : 000B31Effect of storage time on haematological parameters in mullet, Mugil cephalus
Auteurs : C. Faggio ; S. Casella ; F. Arfuso ; S. Marafioti ; G. Piccione ; F. FazioSource :
- Cell Biochemistry and Function [ 0263-6484 ] ; 2013-07.
Abstract
The aim of the present study was to assess the effect of storage time at +4 °C on red blood cell count (RBC), haematocrit (Hct), haemoglobin (Hb), white blood cell count (WBC), thrombocyte count (TC), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) in mullet (Mugil cephalus) using an automatic method. After blood collection (T0), all samples were analyzed using both the manual and automatic method. To test the validation of the automatic method, a paired t‐test was applied, and no statistical difference was observed. The samples were successively divided into four different aliquots and stored at +4 °C to assess the haematological parameters using the automatic method. The first aliquot was refrigerated for 6 h, the second one for 24 h, the third one for 48 h and the last one at for 72 h. One‐way repeated‐measures ANOVA showed a significant effect of storage time (P < 0.05) on Hb, WBC, TC, MCH and MCHC. These results suggest that haematological parameters can be assessed within 6 h from blood collection when samples are stored at +4 °C because long‐term storage modifies the results of the analyses. Further studies on these parameters could be still needed in various fish species to validate an appropriate method for haematological analysis useful not only for the evaluation of the health status of animal living in captivity and in aquaculture but also to have reliability environmental haematological biomarkers. Copyright © 2012 John Wiley & Sons, Ltd.
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DOI: 10.1002/cbf.2915
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ISTEX:AE1B06207CC88A6AD979353555E799E7F43A1F0ALe document en format XML
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Fazio</name><affiliation><mods:affiliation>Faculty of Veterinary Medicine, Department of Experimental Science and Applied Biotechnology, University of Messina, Polo Universitario Annunziata, 98168, Messina, Italy</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Cell Biochemistry and Function</title><title level="j" type="abbrev">Cell Biochem Funct</title><idno type="ISSN">0263-6484</idno><idno type="eISSN">1099-0844</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><date type="published" when="2013-07">2013-07</date><biblScope unit="volume">31</biblScope><biblScope unit="issue">5</biblScope><biblScope unit="page" from="412">412</biblScope><biblScope unit="page" to="416">416</biblScope></imprint><idno type="ISSN">0263-6484</idno></series><idno type="istex">AE1B06207CC88A6AD979353555E799E7F43A1F0A</idno><idno type="DOI">10.1002/cbf.2915</idno><idno type="ArticleID">CBF2915</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0263-6484</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract">The aim of the present study was to assess the effect of storage time at +4 °C on red blood cell count (RBC), haematocrit (Hct), haemoglobin (Hb), white blood cell count (WBC), thrombocyte count (TC), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) in mullet (Mugil cephalus) using an automatic method. After blood collection (T0), all samples were analyzed using both the manual and automatic method. To test the validation of the automatic method, a paired t‐test was applied, and no statistical difference was observed. The samples were successively divided into four different aliquots and stored at +4 °C to assess the haematological parameters using the automatic method. The first aliquot was refrigerated for 6 h, the second one for 24 h, the third one for 48 h and the last one at for 72 h. One‐way repeated‐measures ANOVA showed a significant effect of storage time (P < 0.05) on Hb, WBC, TC, MCH and MCHC. These results suggest that haematological parameters can be assessed within 6 h from blood collection when samples are stored at +4 °C because long‐term storage modifies the results of the analyses. Further studies on these parameters could be still needed in various fish species to validate an appropriate method for haematological analysis useful not only for the evaluation of the health status of animal living in captivity and in aquaculture but also to have reliability environmental haematological biomarkers. Copyright © 2012 John Wiley & Sons, Ltd.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>C. Faggio</name><affiliations><json:string>Faculty of Science, Department of Life Sciences ‘M. Malpighi’ Section of General Physiology, University of Messina, Viale Ferdinando Stagno d'Alcontres, 31 S. Agata, 98166, Messina, Italy</json:string></affiliations></json:item><json:item><name>S. 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Piccione</name><affiliations><json:string>Faculty of Veterinary Medicine, Department of Experimental Science and Applied Biotechnology, University of Messina, Polo Universitario Annunziata, 98168, Messina, Italy</json:string></affiliations></json:item><json:item><name>F. 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After blood collection (T0), all samples were analyzed using both the manual and automatic method. To test the validation of the automatic method, a paired t‐test was applied, and no statistical difference was observed. The samples were successively divided into four different aliquots and stored at +4 °C to assess the haematological parameters using the automatic method. The first aliquot was refrigerated for 6 h, the second one for 24 h, the third one for 48 h and the last one at for 72 h. One‐way repeated‐measures ANOVA showed a significant effect of storage time (P > 0.05) on Hb, WBC, TC, MCH and MCHC. These results suggest that haematological parameters can be assessed within 6 h from blood collection when samples are stored at +4 °C because long‐term storage modifies the results of the analyses. Further studies on these parameters could be still needed in various fish species to validate an appropriate method for haematological analysis useful not only for the evaluation of the health status of animal living in captivity and in aquaculture but also to have reliability environmental haematological biomarkers. 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After blood collection (T0), all samples were analyzed using both the manual and automatic method. To test the validation of the automatic method, a paired t‐test was applied, and no statistical difference was observed. The samples were successively divided into four different aliquots and stored at +4 °C to assess the haematological parameters using the automatic method. The first aliquot was refrigerated for 6 h, the second one for 24 h, the third one for 48 h and the last one at for 72 h. One‐way repeated‐measures ANOVA showed a significant effect of storage time (P < 0.05) on Hb, WBC, TC, MCH and MCHC. These results suggest that haematological parameters can be assessed within 6 h from blood collection when samples are stored at +4 °C because long‐term storage modifies the results of the analyses. Further studies on these parameters could be still needed in various fish species to validate an appropriate method for haematological analysis useful not only for the evaluation of the health status of animal living in captivity and in aquaculture but also to have reliability environmental haematological biomarkers. Copyright © 2012 John Wiley & Sons, Ltd.</p></abstract><textClass><keywords scheme="keyword"><list><head>keywords</head><item><term>haematological automatic analysis</term></item><item><term>haematological parameters</term></item><item><term>Mugil cephalus</term></item><item><term>storage time</term></item><item><term>teleost</term></item></list></keywords></textClass><textClass><keywords scheme="Journal Subject"><list><head>article-category</head><item><term>Research Article</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2012-06-20">Received</change><change when="2012-09-12">Registration</change><change when="2012-09-28">Created</change><change when="2013-07">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/AE1B06207CC88A6AD979353555E799E7F43A1F0A/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component type="serialArticle" version="2.0" xml:lang="en" xml:id="cbf2915"><header><publicationMeta level="product"><doi>10.1002/(ISSN)1099-0844</doi><issn type="print">0263-6484</issn><issn type="electronic">1099-0844</issn><idGroup><id type="product" value="CBF"></id></idGroup><titleGroup><title type="main" sort="CELL BIOCHEMISTRY AND FUNCTION">Cell Biochemistry and Function</title><title type="short">Cell Biochem Funct</title></titleGroup></publicationMeta><publicationMeta level="part" position="50"><doi>10.1002/cbf.v31.5</doi><copyright ownership="publisher">Copyright © 2013 John Wiley & Sons, Ltd.</copyright><numberingGroup><numbering type="journalVolume" number="31">31</numbering><numbering type="journalIssue">5</numbering></numberingGroup><coverDate startDate="2013-07">July 2013</coverDate></publicationMeta><publicationMeta level="unit" position="90" type="article" status="forIssue"><doi>10.1002/cbf.2915</doi><idGroup><id type="unit" value="CBF2915"></id></idGroup><countGroup><count type="pageTotal" number="5"></count></countGroup><titleGroup><title type="articleCategory">Research Article</title><title type="tocHeading1">Research Articles</title></titleGroup><copyright ownership="publisher">Copyright © 2012 John Wiley & Sons, Ltd.</copyright><eventGroup><event type="manuscriptReceived" date="2012-06-20"></event><event type="manuscriptRevised" date="2012-09-06"></event><event type="manuscriptAccepted" date="2012-09-12"></event><event type="xmlCreated" agent="SPi Global" date="2012-09-28"></event><event type="publishedOnlineEarlyUnpaginated" date="2012-10-24"></event><event type="firstOnline" date="2012-10-24"></event><event type="publishedOnlineFinalForm" date="2013-07-02"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:3.8.8" date="2014-01-08"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.6.4 mode:FullText" date="2015-10-03"></event></eventGroup><numberingGroup><numbering type="pageFirst">412</numbering><numbering type="pageLast">416</numbering></numberingGroup><correspondenceTo>Correspondence to: S. Casella, Dipartimento di Scienze Sperimentali e Biotecnologie Applicate, Sezione di Fisiologia Applicata ed Etologia Comparata, Facoltà di Medicina Veterinaria, Università degli Studi di Messina, Polo Universitario dell'Annunziata, 98168 Messina, Italy. E‐mail: <email>stefania.casella@unime.it</email></correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:CBF.CBF2915.pdf"></link></linkGroup></publicationMeta><contentMeta><titleGroup><title type="main">Effect of storage time on haematological parameters in mullet, <i>Mugil cephalus</i></title><title type="short">BLOOD PARAMETERS AND STORAGE TIME E TEMPERATURE IN MULLET</title><title type="shortAuthors">C. FAGGIO <i>ET AL.</i></title></titleGroup><creators><creator creatorRole="author" xml:id="cbf2915-cr-0001" affiliationRef="#cbf2915-aff-0001"><personName><givenNames>C.</givenNames><familyName>Faggio</familyName></personName></creator><creator creatorRole="author" xml:id="cbf2915-cr-0002" affiliationRef="#cbf2915-aff-0002" corresponding="yes"><personName><givenNames>S.</givenNames><familyName>Casella</familyName></personName></creator><creator creatorRole="author" xml:id="cbf2915-cr-0003" affiliationRef="#cbf2915-aff-0002"><personName><givenNames>F.</givenNames><familyName>Arfuso</familyName></personName></creator><creator creatorRole="author" xml:id="cbf2915-cr-0004" affiliationRef="#cbf2915-aff-0002"><personName><givenNames>S.</givenNames><familyName>Marafioti</familyName></personName></creator><creator creatorRole="author" xml:id="cbf2915-cr-0005" affiliationRef="#cbf2915-aff-0002"><personName><givenNames>G.</givenNames><familyName>Piccione</familyName></personName></creator><creator creatorRole="author" xml:id="cbf2915-cr-0006" affiliationRef="#cbf2915-aff-0002"><personName><givenNames>F.</givenNames><familyName>Fazio</familyName></personName></creator></creators><affiliationGroup><affiliation countryCode="IT" type="organization" xml:id="cbf2915-aff-0001"><orgDiv>Faculty of Science, Department of Life Sciences ‘M. Malpighi’ Section of General Physiology</orgDiv><orgName>University of Messina</orgName><address><street>Viale Ferdinando Stagno d'Alcontres, 31 S. Agata</street><postCode>98166</postCode><city>Messina</city><country>Italy</country></address></affiliation><affiliation countryCode="IT" type="organization" xml:id="cbf2915-aff-0002"><orgDiv>Faculty of Veterinary Medicine, Department of Experimental Science and Applied Biotechnology</orgDiv><orgName>University of Messina</orgName><address><street>Polo Universitario Annunziata</street><postCode>98168</postCode><city>Messina</city><country>Italy</country></address></affiliation></affiliationGroup><keywordGroup type="author"><keyword xml:id="cbf2915-kwd-0001">haematological automatic analysis</keyword><keyword xml:id="cbf2915-kwd-0002">haematological parameters</keyword><keyword xml:id="cbf2915-kwd-0003"><i>Mugil cephalus</i></keyword><keyword xml:id="cbf2915-kwd-0004">storage time</keyword><keyword xml:id="cbf2915-kwd-0005">teleost</keyword></keywordGroup><abstractGroup><abstract type="main"><p>The aim of the present study was to assess the effect of storage time at +4 °C on red blood cell count (RBC), haematocrit (Hct), haemoglobin (Hb), white blood cell count (WBC), thrombocyte count (TC), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) in mullet (<i>Mugil cephalus</i>) using an automatic method. After blood collection (<i>T</i><sub>0</sub>), all samples were analyzed using both the manual and automatic method. To test the validation of the automatic method, a paired <i>t</i>‐test was applied, and no statistical difference was observed. The samples were successively divided into four different aliquots and stored at +4 °C to assess the haematological parameters using the automatic method. The first aliquot was refrigerated for 6 h, the second one for 24 h, the third one for 48 h and the last one at for 72 h. One‐way repeated‐measures ANOVA showed a significant effect of storage time (<i>P</i> < 0.05) on Hb, WBC, TC, MCH and MCHC. These results suggest that haematological parameters can be assessed within 6 h from blood collection when samples are stored at +4 °C because long‐term storage modifies the results of the analyses. Further studies on these parameters could be still needed in various fish species to validate an appropriate method for haematological analysis useful not only for the evaluation of the health status of animal living in captivity and in aquaculture but also to have reliability environmental haematological biomarkers. Copyright © 2012 John Wiley & Sons, Ltd.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Effect of storage time on haematological parameters in mullet, Mugil cephalus</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>BLOOD PARAMETERS AND STORAGE TIME E TEMPERATURE IN MULLET</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Effect of storage time on haematological parameters in mullet, Mugil cephalus</title></titleInfo><name type="personal"><namePart type="given">C.</namePart><namePart type="family">Faggio</namePart><affiliation>Faculty of Science, Department of Life Sciences ‘M. Malpighi’ Section of General Physiology, University of Messina, Viale Ferdinando Stagno d'Alcontres, 31 S. Agata, 98166, Messina, Italy</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S.</namePart><namePart type="family">Casella</namePart><affiliation>Faculty of Veterinary Medicine, Department of Experimental Science and Applied Biotechnology, University of Messina, Polo Universitario Annunziata, 98168, Messina, Italy</affiliation><affiliation>E-mail: stefania.casella@unime.it</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">F.</namePart><namePart type="family">Arfuso</namePart><affiliation>Faculty of Veterinary Medicine, Department of Experimental Science and Applied Biotechnology, University of Messina, Polo Universitario Annunziata, 98168, Messina, Italy</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S.</namePart><namePart type="family">Marafioti</namePart><affiliation>Faculty of Veterinary Medicine, Department of Experimental Science and Applied Biotechnology, University of Messina, Polo Universitario Annunziata, 98168, Messina, Italy</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">G.</namePart><namePart type="family">Piccione</namePart><affiliation>Faculty of Veterinary Medicine, Department of Experimental Science and Applied Biotechnology, University of Messina, Polo Universitario Annunziata, 98168, Messina, Italy</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">F.</namePart><namePart type="family">Fazio</namePart><affiliation>Faculty of Veterinary Medicine, Department of Experimental Science and Applied Biotechnology, University of Messina, Polo Universitario Annunziata, 98168, Messina, Italy</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Blackwell Publishing Ltd</publisher><dateIssued encoding="w3cdtf">2013-07</dateIssued><dateCreated encoding="w3cdtf">2012-09-28</dateCreated><dateCaptured encoding="w3cdtf">2012-06-20</dateCaptured><dateValid encoding="w3cdtf">2012-09-12</dateValid><copyrightDate encoding="w3cdtf">2013</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract>The aim of the present study was to assess the effect of storage time at +4 °C on red blood cell count (RBC), haematocrit (Hct), haemoglobin (Hb), white blood cell count (WBC), thrombocyte count (TC), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) in mullet (Mugil cephalus) using an automatic method. After blood collection (T0), all samples were analyzed using both the manual and automatic method. To test the validation of the automatic method, a paired t‐test was applied, and no statistical difference was observed. The samples were successively divided into four different aliquots and stored at +4 °C to assess the haematological parameters using the automatic method. The first aliquot was refrigerated for 6 h, the second one for 24 h, the third one for 48 h and the last one at for 72 h. One‐way repeated‐measures ANOVA showed a significant effect of storage time (P < 0.05) on Hb, WBC, TC, MCH and MCHC. These results suggest that haematological parameters can be assessed within 6 h from blood collection when samples are stored at +4 °C because long‐term storage modifies the results of the analyses. Further studies on these parameters could be still needed in various fish species to validate an appropriate method for haematological analysis useful not only for the evaluation of the health status of animal living in captivity and in aquaculture but also to have reliability environmental haematological biomarkers. Copyright © 2012 John Wiley & Sons, Ltd.</abstract><subject><genre>keywords</genre><topic>haematological automatic analysis</topic><topic>haematological parameters</topic><topic>Mugil cephalus</topic><topic>storage time</topic><topic>teleost</topic></subject><relatedItem type="host"><titleInfo><title>Cell Biochemistry and Function</title></titleInfo><titleInfo type="abbreviated"><title>Cell Biochem Funct</title></titleInfo><genre type="journal">journal</genre><subject><genre>article-category</genre><topic>Research Article</topic></subject><identifier type="ISSN">0263-6484</identifier><identifier type="eISSN">1099-0844</identifier><identifier type="DOI">10.1002/(ISSN)1099-0844</identifier><identifier type="PublisherID">CBF</identifier><part><date>2013</date><detail type="volume"><caption>vol.</caption><number>31</number></detail><detail type="issue"><caption>no.</caption><number>5</number></detail><extent unit="pages"><start>412</start><end>416</end><total>5</total></extent></part></relatedItem><identifier type="istex">AE1B06207CC88A6AD979353555E799E7F43A1F0A</identifier><identifier type="DOI">10.1002/cbf.2915</identifier><identifier type="ArticleID">CBF2915</identifier><accessCondition type="use and reproduction" contentType="copyright">Copyright © 2013 John Wiley & Sons, Ltd.Copyright © 2012 John Wiley & Sons, Ltd.</accessCondition><recordInfo><recordContentSource>WILEY</recordContentSource></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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