Specificity of antibodies established from mammals in rainbow trout (Oncorhynchus mykiss)
Identifieur interne : 000B16 ( Istex/Corpus ); précédent : 000B15; suivant : 000B17Specificity of antibodies established from mammals in rainbow trout (Oncorhynchus mykiss)
Auteurs : B. Grunow ; B. Böhmert ; K. FechnerSource :
- Journal of Applied Ichthyology [ 0175-8659 ] ; 2013-10.
Abstract
This study deals with the question as to whether antibodies established for mammals are also specific for rainbow trout. The reason for this examination is the major economic importance of rainbow trout in aquaculture and the growing scientific attention. However, there are few primary antibodies so far defined for this fish species. Therefore, the aim of the current study was to test the ability of 15 commercially available antibodies for rainbow trout in an indirect immunofluoresence assay to analyse tissue sections of organs. Five commercially available primary antibodies were identified, which were directed against proteins of one or two organs/ cell types in rainbow trout.
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DOI: 10.1111/jai.12146
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Grunow</name><affiliation><mods:affiliation>Fraunhofer Research Institution for Marine Biotechnology, Fraunhofer Society, Lübeck, Germany</mods:affiliation></affiliation><affiliation><mods:affiliation>Dr. Bianka Grunow, Fraunhofer Research Institution for Marine Biotechnology, Fraunhofer Society, Paul Ehrlich Str. 1‐3, D‐23562 Lübeck, Germany.E‐mail:</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: bianka.grunow@emb.fraunhofer.de</mods:affiliation></affiliation></author><author><name sortKey="Bohmert, B" sort="Bohmert, B" uniqKey="Bohmert B" first="B." last="Böhmert">B. Böhmert</name><affiliation><mods:affiliation>Fraunhofer Research Institution for Marine Biotechnology, Fraunhofer Society, Lübeck, Germany</mods:affiliation></affiliation></author><author><name sortKey="Fechner, K" sort="Fechner, K" uniqKey="Fechner K" first="K." last="Fechner">K. 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Appl. Ichthyol.</title><idno type="pISSN">0175-8659</idno><idno type="eISSN">1439-0426</idno><idno type="DOI">10.1111/(ISSN)1439-0426</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><date type="published" when="2013-10"></date><biblScope unit="volume">29</biblScope><biblScope unit="issue">5</biblScope><biblScope unit="page" from="1129">1129</biblScope><biblScope unit="page" to="1133">1133</biblScope></imprint></monogr><idno type="istex">EFB41357A261456D8649320C64931D5E4DF5ABA8</idno><idno type="DOI">10.1111/jai.12146</idno><idno type="ArticleID">JAI12146</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2012-11-27</date></creation><langUsage><language ident="en">en</language></langUsage><abstract><p>This study deals with the question as to whether antibodies established for mammals are also specific for rainbow trout. 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Ichthyol.</title></titleGroup></publicationMeta><publicationMeta level="part" position="10105"><doi origin="wiley">10.1111/jai.2013.29.issue-5</doi><copyright ownership="publisher">Copyright © 2013 Blackwell Verlag GmbH</copyright><numberingGroup><numbering type="journalVolume" number="29">29</numbering><numbering type="journalIssue">5</numbering></numberingGroup><coverDate startDate="2013-10">October 2013</coverDate></publicationMeta><publicationMeta level="unit" position="280" status="forIssue" type="article"><doi>10.1111/jai.12146</doi><idGroup><id type="unit" value="JAI12146"></id></idGroup><countGroup><count number="5" type="pageTotal"></count></countGroup><titleGroup><title type="articleCategory">Original Article</title><title type="tocHeading1">Original Articles</title></titleGroup><copyright ownership="publisher">© 2013 Blackwell Verlag GmbH</copyright><eventGroup><event date="2012-05-10" type="manuscriptReceived"></event><event date="2012-10-26" type="manuscriptAccepted"></event><event agent="SPS" date="2012-11-27" type="xmlCreated"></event><event type="publishedOnlineEarlyUnpaginated" date="2013-02-02"></event><event type="firstOnline" date="2013-02-02"></event><event type="publishedOnlineFinalForm" date="2013-09-10"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:3.8.8" date="2014-01-28"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.6.4 mode:FullText" date="2015-10-01"></event></eventGroup><numberingGroup><numbering type="pageFirst">1129</numbering><numbering type="pageLast">1133</numbering></numberingGroup><correspondenceTo><lineatedText><line><b>Author's address:</b> Dr. Bianka Grunow, Fraunhofer Research Institution for Marine Biotechnology, Fraunhofer Society, Paul Ehrlich Str. 1‐3, D‐23562 Lübeck, Germany.</line><line>E‐mail: <email>bianka.grunow@emb.fraunhofer.de</email></line></lineatedText></correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:JAI.JAI12146.pdf"></link></linkGroup></publicationMeta><contentMeta><titleGroup><title type="main">Specificity of antibodies established from mammals in rainbow trout (<i>Oncorhynchus mykiss)</i></title><title type="shortAuthors">B. Grunow, B. Böhmert and K. Fechner</title></titleGroup><creators><creator affiliationRef="#jai12146-aff-0001" corresponding="yes" creatorRole="author" xml:id="jai12146-cr-0001"><personName><givenNames>B.</givenNames> <familyName>Grunow</familyName></personName></creator><creator affiliationRef="#jai12146-aff-0001" creatorRole="author" xml:id="jai12146-cr-0002"><personName><givenNames>B.</givenNames> <familyName>Böhmert</familyName></personName></creator><creator affiliationRef="#jai12146-aff-0002" creatorRole="author" xml:id="jai12146-cr-0003"><personName><givenNames>K.</givenNames> <familyName>Fechner</familyName></personName></creator></creators><affiliationGroup><affiliation countryCode="DE" type="organization" xml:id="jai12146-aff-0001"><orgName>Fraunhofer Research Institution for Marine Biotechnology, Fraunhofer Society</orgName> <address><city>Lübeck</city> <country>Germany</country></address></affiliation><affiliation countryCode="DE" type="organization" xml:id="jai12146-aff-0002"><orgName>Euroimmun AG</orgName> <address><city>Lübeck</city> <country>Germany</country></address></affiliation></affiliationGroup><fundingInfo><fundingAgency>Ministry of Science, Economy and Transport of Schleswig‐Holstein</fundingAgency></fundingInfo><fundingInfo><fundingAgency>European Regional Development Fund (RDF)</fundingAgency></fundingInfo><abstractGroup><abstract type="main" xml:id="jai12146-abs-0001"><title type="main">Summary</title><p>This study deals with the question as to whether antibodies established for mammals are also specific for rainbow trout. The reason for this examination is the major economic importance of rainbow trout in aquaculture and the growing scientific attention. However, there are few primary antibodies so far defined for this fish species. Therefore, the aim of the current study was to test the ability of 15 commercially available antibodies for rainbow trout in an indirect immunofluoresence assay to analyse tissue sections of organs. 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The reason for this examination is the major economic importance of rainbow trout in aquaculture and the growing scientific attention. However, there are few primary antibodies so far defined for this fish species. Therefore, the aim of the current study was to test the ability of 15 commercially available antibodies for rainbow trout in an indirect immunofluoresence assay to analyse tissue sections of organs. Five commercially available primary antibodies were identified, which were directed against proteins of one or two organs/ cell types in rainbow trout.</abstract><note type="funding">Ministry of Science, Economy and Transport of Schleswig‐Holstein</note><note type="funding">European Regional Development Fund (RDF)</note><relatedItem type="host"><titleInfo><title>Journal of Applied Ichthyology</title></titleInfo><titleInfo type="abbreviated"><title>J. Appl. 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