Enzyme immunoassay for 11-ketotestosterone using acetylcholinesterase as laberl: application to the measurement of 11-ketotestosterone in plasma of Siberian sturgeon
Identifieur interne : 000B09 ( Istex/Corpus ); précédent : 000B08; suivant : 000B10Enzyme immunoassay for 11-ketotestosterone using acetylcholinesterase as laberl: application to the measurement of 11-ketotestosterone in plasma of Siberian sturgeon
Auteurs : B. Cuisset ; P. Pradelles ; D. E. Kime ; E. R. Kühn ; P. Babin ; S. Davail ; F. Le MennSource :
- Comparative Biochemistry and Physiology, Part C: Comparative Pharmacology and Toxicology [ 0742-8413 ] ; 1994.
Abstract
A competitive enzyme immunoassay using 11-ketotestosterone (11-KT) covalently coupled to acetylcholinesterase (AChE) from electric eel is developed. Ninety-six well microtiter plates are coated with pure mouse-anti-rabbit-IgG monoclonal antibody and competition is performed in one-step reaction by adding successively 11-KT standard or samples, enzymatic tracer and 11-KT-specific antiserum. This assay is more sensitive (detection limit is 7 pg/ml) than radioimmunoassays previously described, and specificity was not modified by the use of enzymatic tracer. This assay can be performed in less than 5 hr. Validation of the EIA was achieved in a fish model, Siberian sturgeon (Acipenser baeri), by comparative determinations of 11-KT levels in plasma extracts using EIA and RIA. Application to the measurement of 11-KT in plasma of farmed Siberian sturgeon during the first reproductive cycle is reported.
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DOI: 10.1016/1367-8280(94)90035-3
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Cuisset</name><affiliation><mods:affiliation>UA-INRA 785.2 Biologie de la Reproduction des Poissons, Université Bordeaux I, Av. des Facultés, 33405 Talence, France</mods:affiliation></affiliation><affiliation><mods:affiliation>Correspondence to: B. Cuisset, UA-INRA 785.2 Biologie de la Reproduction des Poissons, Universite Bordeaux I, Av. des Facultes, 33405 Talence, France. Tel. 56848803; Fax 56848701.</mods:affiliation></affiliation></author><author><name sortKey="Pradelles, P" sort="Pradelles, P" uniqKey="Pradelles P" first="P." last="Pradelles">P. Pradelles</name><affiliation><mods:affiliation>Section de Pharmacologie et Immunologie, Département de Biologie, CE/Saclay, 91191 Gif sur Yvette, France</mods:affiliation></affiliation></author><author><name sortKey="Kime, D E" sort="Kime, D E" uniqKey="Kime D" first="D. E." last="Kime">D. E. Kime</name><affiliation><mods:affiliation>Department of Animal and Plant Sciences, University of Sheffield, Sheffield S10 2UQ, U.K.</mods:affiliation></affiliation></author><author><name sortKey="Kuhn, E R" sort="Kuhn, E R" uniqKey="Kuhn E" first="E. R." last="Kühn">E. R. Kühn</name><affiliation><mods:affiliation>Laboratory for Comparative Endocrinology, Catholic University of Leuven, Naamsestraat 61, B-3000 Leuven, Belgium</mods:affiliation></affiliation></author><author><name sortKey="Babin, P" sort="Babin, P" uniqKey="Babin P" first="P." last="Babin">P. Babin</name><affiliation><mods:affiliation>Laboratoire de Chimie Organique et Organométallique, UERA 35 CNRS, Université Bordeaux I, 254 Cours de la Libération, 33405 Talence, France</mods:affiliation></affiliation></author><author><name sortKey="Davail, S" sort="Davail, S" uniqKey="Davail S" first="S." last="Davail">S. Davail</name><affiliation><mods:affiliation>UA-INRA 785.2 Biologie de la Reproduction des Poissons, Université Bordeaux I, Av. des Facultés, 33405 Talence, France</mods:affiliation></affiliation></author><author><name sortKey="Le Menn, F" sort="Le Menn, F" uniqKey="Le Menn F" first="F." last="Le Menn">F. Le Menn</name><affiliation><mods:affiliation>UA-INRA 785.2 Biologie de la Reproduction des Poissons, Université Bordeaux I, Av. des Facultés, 33405 Talence, France</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Comparative Biochemistry and Physiology, Part C: Comparative Pharmacology and Toxicology</title><title level="j" type="abbrev">CBCOLD</title><idno type="ISSN">0742-8413</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1994">1994</date><biblScope unit="volume">108</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="229">229</biblScope><biblScope unit="page" to="241">241</biblScope></imprint><idno type="ISSN">0742-8413</idno></series><idno type="istex">2339DDE2BE1B2F5DC5B43F018C019D15114AB730</idno><idno type="DOI">10.1016/1367-8280(94)90035-3</idno><idno type="PII">1367-8280(94)90035-3</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0742-8413</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A competitive enzyme immunoassay using 11-ketotestosterone (11-KT) covalently coupled to acetylcholinesterase (AChE) from electric eel is developed. Ninety-six well microtiter plates are coated with pure mouse-anti-rabbit-IgG monoclonal antibody and competition is performed in one-step reaction by adding successively 11-KT standard or samples, enzymatic tracer and 11-KT-specific antiserum. This assay is more sensitive (detection limit is 7 pg/ml) than radioimmunoassays previously described, and specificity was not modified by the use of enzymatic tracer. This assay can be performed in less than 5 hr. Validation of the EIA was achieved in a fish model, Siberian sturgeon (Acipenser baeri), by comparative determinations of 11-KT levels in plasma extracts using EIA and RIA. Application to the measurement of 11-KT in plasma of farmed Siberian sturgeon during the first reproductive cycle is reported.</div></front></TEI><istex><corpusName>elsevier</corpusName><author><json:item><name>B. Cuisset</name><affiliations><json:string>UA-INRA 785.2 Biologie de la Reproduction des Poissons, Université Bordeaux I, Av. des Facultés, 33405 Talence, France</json:string><json:string>Correspondence to: B. Cuisset, UA-INRA 785.2 Biologie de la Reproduction des Poissons, Universite Bordeaux I, Av. des Facultes, 33405 Talence, France. Tel. 56848803; Fax 56848701.</json:string></affiliations></json:item><json:item><name>P. Pradelles</name><affiliations><json:string>Section de Pharmacologie et Immunologie, Département de Biologie, CE/Saclay, 91191 Gif sur Yvette, France</json:string></affiliations></json:item><json:item><name>D.E. Kime</name><affiliations><json:string>Department of Animal and Plant Sciences, University of Sheffield, Sheffield S10 2UQ, U.K.</json:string></affiliations></json:item><json:item><name>E.R. Kühn</name><affiliations><json:string>Laboratory for Comparative Endocrinology, Catholic University of Leuven, Naamsestraat 61, B-3000 Leuven, Belgium</json:string></affiliations></json:item><json:item><name>P. Babin</name><affiliations><json:string>Laboratoire de Chimie Organique et Organométallique, UERA 35 CNRS, Université Bordeaux I, 254 Cours de la Libération, 33405 Talence, France</json:string></affiliations></json:item><json:item><name>S. Davail</name><affiliations><json:string>UA-INRA 785.2 Biologie de la Reproduction des Poissons, Université Bordeaux I, Av. des Facultés, 33405 Talence, France</json:string></affiliations></json:item><json:item><name>F. Le Menn</name><affiliations><json:string>UA-INRA 785.2 Biologie de la Reproduction des Poissons, Université Bordeaux I, Av. des Facultés, 33405 Talence, France</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Acetylcholinesterase</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>11-Ketotestosterone</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Plasma</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Siberian sturgeon</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Enzyme immunoassay</value></json:item></subject><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>A competitive enzyme immunoassay using 11-ketotestosterone (11-KT) covalently coupled to acetylcholinesterase (AChE) from electric eel is developed. Ninety-six well microtiter plates are coated with pure mouse-anti-rabbit-IgG monoclonal antibody and competition is performed in one-step reaction by adding successively 11-KT standard or samples, enzymatic tracer and 11-KT-specific antiserum. This assay is more sensitive (detection limit is 7 pg/ml) than radioimmunoassays previously described, and specificity was not modified by the use of enzymatic tracer. This assay can be performed in less than 5 hr. Validation of the EIA was achieved in a fish model, Siberian sturgeon (Acipenser baeri), by comparative determinations of 11-KT levels in plasma extracts using EIA and RIA. Application to the measurement of 11-KT in plasma of farmed Siberian sturgeon during the first reproductive cycle is reported.</abstract><qualityIndicators><score>6.536</score><pdfVersion>1.2</pdfVersion><pdfPageSize>546 x 771 pts</pdfPageSize><refBibsNative>true</refBibsNative><keywordCount>5</keywordCount><abstractCharCount>907</abstractCharCount><pdfWordCount>5838</pdfWordCount><pdfCharCount>38273</pdfCharCount><pdfPageCount>13</pdfPageCount><abstractWordCount>128</abstractWordCount></qualityIndicators><title>Enzyme immunoassay for 11-ketotestosterone using acetylcholinesterase as laberl: application to the measurement of 11-ketotestosterone in plasma of Siberian sturgeon</title><pii><json:string>1367-8280(94)90035-3</json:string></pii><genre><json:string>research-article</json:string></genre><serie><volume>Vol. IXA</volume><pages><last>372</last><first>277</first></pages><language><json:string>unknown</json:string></language><title>Fish Physiology</title></serie><host><volume>108</volume><pii><json:string>S1367-8280(00)X0003-2</json:string></pii><pages><last>241</last><first>229</first></pages><issn><json:string>0742-8413</json:string></issn><issue>2</issue><genre><json:string>journal</json:string></genre><language><json:string>unknown</json:string></language><title>Comparative Biochemistry and Physiology, Part C: Comparative Pharmacology and Toxicology</title><publicationDate>1994</publicationDate></host><publicationDate>1994</publicationDate><copyrightDate>1994</copyrightDate><doi><json:string>10.1016/1367-8280(94)90035-3</json:string></doi><id>2339DDE2BE1B2F5DC5B43F018C019D15114AB730</id><score>0.06351157</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/document/2339DDE2BE1B2F5DC5B43F018C019D15114AB730/fulltext/pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/document/2339DDE2BE1B2F5DC5B43F018C019D15114AB730/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/2339DDE2BE1B2F5DC5B43F018C019D15114AB730/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a">Enzyme immunoassay for 11-ketotestosterone using acetylcholinesterase as laberl: application to the measurement of 11-ketotestosterone in plasma of Siberian sturgeon</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>ELSEVIER</publisher><availability><p>ELSEVIER</p></availability><date>1994</date></publicationStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">Enzyme immunoassay for 11-ketotestosterone using acetylcholinesterase as laberl: application to the measurement of 11-ketotestosterone in plasma of Siberian sturgeon</title><author xml:id="author-1"><persName><forename type="first">B.</forename><surname>Cuisset</surname></persName><affiliation>UA-INRA 785.2 Biologie de la Reproduction des Poissons, Université Bordeaux I, Av. des Facultés, 33405 Talence, France</affiliation><affiliation>Correspondence to: B. Cuisset, UA-INRA 785.2 Biologie de la Reproduction des Poissons, Universite Bordeaux I, Av. des Facultes, 33405 Talence, France. Tel. 56848803; Fax 56848701.</affiliation></author><author xml:id="author-2"><persName><forename type="first">P.</forename><surname>Pradelles</surname></persName><affiliation>Section de Pharmacologie et Immunologie, Département de Biologie, CE/Saclay, 91191 Gif sur Yvette, France</affiliation></author><author xml:id="author-3"><persName><forename type="first">D.E.</forename><surname>Kime</surname></persName><affiliation>Department of Animal and Plant Sciences, University of Sheffield, Sheffield S10 2UQ, U.K.</affiliation></author><author xml:id="author-4"><persName><forename type="first">E.R.</forename><surname>Kühn</surname></persName><affiliation>Laboratory for Comparative Endocrinology, Catholic University of Leuven, Naamsestraat 61, B-3000 Leuven, Belgium</affiliation></author><author xml:id="author-5"><persName><forename type="first">P.</forename><surname>Babin</surname></persName><affiliation>Laboratoire de Chimie Organique et Organométallique, UERA 35 CNRS, Université Bordeaux I, 254 Cours de la Libération, 33405 Talence, France</affiliation></author><author xml:id="author-6"><persName><forename type="first">S.</forename><surname>Davail</surname></persName><affiliation>UA-INRA 785.2 Biologie de la Reproduction des Poissons, Université Bordeaux I, Av. des Facultés, 33405 Talence, France</affiliation></author><author xml:id="author-7"><persName><forename type="first">F.</forename><surname>Le Menn</surname></persName><affiliation>UA-INRA 785.2 Biologie de la Reproduction des Poissons, Université Bordeaux I, Av. des Facultés, 33405 Talence, France</affiliation></author></analytic><monogr><title level="j">Comparative Biochemistry and Physiology, Part C: Comparative Pharmacology and Toxicology</title><title level="j" type="abbrev">CBCOLD</title><idno type="pISSN">0742-8413</idno><idno type="PII">S1367-8280(00)X0003-2</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1994"></date><biblScope unit="volume">108</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="229">229</biblScope><biblScope unit="page" to="241">241</biblScope></imprint></monogr><idno type="istex">2339DDE2BE1B2F5DC5B43F018C019D15114AB730</idno><idno type="DOI">10.1016/1367-8280(94)90035-3</idno><idno type="PII">1367-8280(94)90035-3</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1994</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>A competitive enzyme immunoassay using 11-ketotestosterone (11-KT) covalently coupled to acetylcholinesterase (AChE) from electric eel is developed. Ninety-six well microtiter plates are coated with pure mouse-anti-rabbit-IgG monoclonal antibody and competition is performed in one-step reaction by adding successively 11-KT standard or samples, enzymatic tracer and 11-KT-specific antiserum. This assay is more sensitive (detection limit is 7 pg/ml) than radioimmunoassays previously described, and specificity was not modified by the use of enzymatic tracer. This assay can be performed in less than 5 hr. Validation of the EIA was achieved in a fish model, Siberian sturgeon (Acipenser baeri), by comparative determinations of 11-KT levels in plasma extracts using EIA and RIA. Application to the measurement of 11-KT in plasma of farmed Siberian sturgeon during the first reproductive cycle is reported.</p></abstract><textClass><keywords scheme="keyword"><list><head>Keywords</head><item><term>Acetylcholinesterase</term></item><item><term>11-Ketotestosterone</term></item><item><term>Plasma</term></item><item><term>Siberian sturgeon</term></item><item><term>Enzyme immunoassay</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1994">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/2339DDE2BE1B2F5DC5B43F018C019D15114AB730/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>CBCOLD</jid><aid>94900353</aid><ce:pii>1367-8280(94)90035-3</ce:pii><ce:doi>10.1016/1367-8280(94)90035-3</ce:doi><ce:copyright type="unknown" year="1994"></ce:copyright></item-info><head><ce:title>Enzyme immunoassay for 11-ketotestosterone using acetylcholinesterase as laberl: application to the measurement of 11-ketotestosterone in plasma of Siberian sturgeon</ce:title><ce:author-group><ce:author><ce:given-name>B.</ce:given-name><ce:surname>Cuisset</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>∗</ce:sup></ce:cross-ref><ce:cross-ref refid="COR1"><ce:sup>1</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>P.</ce:given-name><ce:surname>Pradelles</ce:surname><ce:cross-ref refid="AFF2"><ce:sup>†</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>D.E.</ce:given-name><ce:surname>Kime</ce:surname><ce:cross-ref refid="AFF3"><ce:sup>‡</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>E.R.</ce:given-name><ce:surname>Kühn</ce:surname><ce:cross-ref refid="AFF4"><ce:sup>§</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>P.</ce:given-name><ce:surname>Babin</ce:surname><ce:cross-ref refid="AFF5"><ce:sup>∥</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>S.</ce:given-name><ce:surname>Davail</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>∗</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>F.</ce:given-name><ce:surname>Le Menn</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>∗</ce:sup></ce:cross-ref></ce:author><ce:affiliation id="AFF1"><ce:label>∗</ce:label><ce:textfn>UA-INRA 785.2 Biologie de la Reproduction des Poissons, Université Bordeaux I, Av. des Facultés, 33405 Talence, France</ce:textfn></ce:affiliation><ce:affiliation id="AFF2"><ce:label>†</ce:label><ce:textfn>Section de Pharmacologie et Immunologie, Département de Biologie, CE/Saclay, 91191 Gif sur Yvette, France</ce:textfn></ce:affiliation><ce:affiliation id="AFF3"><ce:label>‡</ce:label><ce:textfn>Department of Animal and Plant Sciences, University of Sheffield, Sheffield S10 2UQ, U.K.</ce:textfn></ce:affiliation><ce:affiliation id="AFF4"><ce:label>§</ce:label><ce:textfn>Laboratory for Comparative Endocrinology, Catholic University of Leuven, Naamsestraat 61, B-3000 Leuven, Belgium</ce:textfn></ce:affiliation><ce:affiliation id="AFF5"><ce:label>∥</ce:label><ce:textfn>Laboratoire de Chimie Organique et Organométallique, UERA 35 CNRS, Université Bordeaux I, 254 Cours de la Libération, 33405 Talence, France</ce:textfn></ce:affiliation><ce:correspondence id="COR1"><ce:label>1</ce:label><ce:text>Correspondence to: B. Cuisset, UA-INRA 785.2 Biologie de la Reproduction des Poissons, Universite Bordeaux I, Av. des Facultes, 33405 Talence, France. Tel. 56848803; Fax 56848701.</ce:text></ce:correspondence></ce:author-group><ce:date-received day="23" month="9" year="1993"></ce:date-received><ce:date-accepted day="2" month="2" year="1994"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>A competitive enzyme immunoassay using 11-ketotestosterone (11-KT) covalently coupled to acetylcholinesterase (AChE) from electric eel is developed. Ninety-six well microtiter plates are coated with pure mouse-anti-rabbit-IgG monoclonal antibody and competition is performed in one-step reaction by adding successively 11-KT standard or samples, enzymatic tracer and 11-KT-specific antiserum. This assay is more sensitive (detection limit is 7 pg/ml) than radioimmunoassays previously described, and specificity was not modified by the use of enzymatic tracer. This assay can be performed in less than 5 hr. Validation of the EIA was achieved in a fish model, Siberian sturgeon (<ce:italic>Acipenser baeri</ce:italic>), by comparative determinations of 11-KT levels in plasma extracts using EIA and RIA. Application to the measurement of 11-KT in plasma of farmed Siberian sturgeon during the first reproductive cycle is reported.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords><ce:section-title>Keywords</ce:section-title><ce:keyword><ce:text>Acetylcholinesterase</ce:text></ce:keyword><ce:keyword><ce:text>11-Ketotestosterone</ce:text></ce:keyword><ce:keyword><ce:text>Plasma</ce:text></ce:keyword><ce:keyword><ce:text>Siberian sturgeon</ce:text></ce:keyword><ce:keyword><ce:text>Enzyme immunoassay</ce:text></ce:keyword></ce:keywords></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Enzyme immunoassay for 11-ketotestosterone using acetylcholinesterase as laberl: application to the measurement of 11-ketotestosterone in plasma of Siberian sturgeon</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Enzyme immunoassay for 11-ketotestosterone using acetylcholinesterase as laberl: application to the measurement of 11-ketotestosterone in plasma of Siberian sturgeon</title></titleInfo><name type="personal"><namePart type="given">B.</namePart><namePart type="family">Cuisset</namePart><affiliation>UA-INRA 785.2 Biologie de la Reproduction des Poissons, Université Bordeaux I, Av. des Facultés, 33405 Talence, France</affiliation><affiliation>Correspondence to: B. Cuisset, UA-INRA 785.2 Biologie de la Reproduction des Poissons, Universite Bordeaux I, Av. des Facultes, 33405 Talence, France. Tel. 56848803; Fax 56848701.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">P.</namePart><namePart type="family">Pradelles</namePart><affiliation>Section de Pharmacologie et Immunologie, Département de Biologie, CE/Saclay, 91191 Gif sur Yvette, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">D.E.</namePart><namePart type="family">Kime</namePart><affiliation>Department of Animal and Plant Sciences, University of Sheffield, Sheffield S10 2UQ, U.K.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">E.R.</namePart><namePart type="family">Kühn</namePart><affiliation>Laboratory for Comparative Endocrinology, Catholic University of Leuven, Naamsestraat 61, B-3000 Leuven, Belgium</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">P.</namePart><namePart type="family">Babin</namePart><affiliation>Laboratoire de Chimie Organique et Organométallique, UERA 35 CNRS, Université Bordeaux I, 254 Cours de la Libération, 33405 Talence, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S.</namePart><namePart type="family">Davail</namePart><affiliation>UA-INRA 785.2 Biologie de la Reproduction des Poissons, Université Bordeaux I, Av. des Facultés, 33405 Talence, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">F.</namePart><namePart type="family">Le Menn</namePart><affiliation>UA-INRA 785.2 Biologie de la Reproduction des Poissons, Université Bordeaux I, Av. des Facultés, 33405 Talence, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article"></genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1994</dateIssued><copyrightDate encoding="w3cdtf">1994</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">A competitive enzyme immunoassay using 11-ketotestosterone (11-KT) covalently coupled to acetylcholinesterase (AChE) from electric eel is developed. Ninety-six well microtiter plates are coated with pure mouse-anti-rabbit-IgG monoclonal antibody and competition is performed in one-step reaction by adding successively 11-KT standard or samples, enzymatic tracer and 11-KT-specific antiserum. This assay is more sensitive (detection limit is 7 pg/ml) than radioimmunoassays previously described, and specificity was not modified by the use of enzymatic tracer. This assay can be performed in less than 5 hr. Validation of the EIA was achieved in a fish model, Siberian sturgeon (Acipenser baeri), by comparative determinations of 11-KT levels in plasma extracts using EIA and RIA. Application to the measurement of 11-KT in plasma of farmed Siberian sturgeon during the first reproductive cycle is reported.</abstract><subject><genre>Keywords</genre><topic>Acetylcholinesterase</topic><topic>11-Ketotestosterone</topic><topic>Plasma</topic><topic>Siberian sturgeon</topic><topic>Enzyme immunoassay</topic></subject><relatedItem type="host"><titleInfo><title>Comparative Biochemistry and Physiology, Part C: Comparative Pharmacology and Toxicology</title></titleInfo><titleInfo type="abbreviated"><title>CBCOLD</title></titleInfo><genre type="journal">journal</genre><originInfo><dateIssued encoding="w3cdtf">199407</dateIssued></originInfo><identifier type="ISSN">0742-8413</identifier><identifier type="PII">S1367-8280(00)X0003-2</identifier><part><date>199407</date><detail type="volume"><number>108</number><caption>vol.</caption></detail><detail type="issue"><number>2</number><caption>no.</caption></detail><extent unit="issue pages"><start>137</start><end>257</end></extent><extent unit="pages"><start>229</start><end>241</end></extent></part></relatedItem><identifier type="istex">2339DDE2BE1B2F5DC5B43F018C019D15114AB730</identifier><identifier type="DOI">10.1016/1367-8280(94)90035-3</identifier><identifier type="PII">1367-8280(94)90035-3</identifier><recordInfo><recordContentSource>ELSEVIER</recordContentSource></recordInfo></mods></metadata></istex></record>Pour manipuler ce document sous Unix (Dilib)
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