Motility analysis and energetics of the Siberian sturgeon Acipenser baerii spermatozoa
Identifieur interne : 000A39 ( Istex/Corpus ); précédent : 000A38; suivant : 000A40Motility analysis and energetics of the Siberian sturgeon Acipenser baerii spermatozoa
Auteurs : R. Billard ; J. Cosson ; F. Fierville ; R. Brun ; T. Rouault ; P. WilliotSource :
- Journal of Applied Ichthyology [ 0175-8659 ] ; 1999-09.
Abstract
A study of motility was carried out on spermatozoa of the Siberian sturgeon (Acipenser baerii Brandt) reared at the Cemagref experimental research station, St Seurin sur l'Isle, France. About 90% spermatozoa are activated within a few seconds after a double step dilution (final dilution 1/600) and the number of motile cells declines regularly to 10–20% after 2 mn. During the same period of time, the flagellum beat frequency remains stable at about 50 Hz for 30 sec and drops to 30 Hz after 60 sec. A morphological analysis of the movement of the flagellum shows that during the first 20 sec, waves are distributed on the entire length of the flagellum; later, they disappear progressively from the distal part within 1 mn in the case of 90% of the spermatozoa. Finally after 2 mn, most of the flagella are straight and devoid of wave. The ATP content declines very rapidly after activation from 7.58 ± 1.85 to 4.05 ± 1.51 nmol/108 spermatozoa within 5 sec (P=0.004). Spermatozoa left in the genital tract of the males over a period of 24 hrs (in vivo survival) do not show significant change in the percentage of motile cells (82 ± 14 vs 74 ± 15) nor in the ATP content (6.13 ± 2.37 vs 4.59 ± 1.42 nmoles/108 spermatozoa). An aliquot of these spermatozoa stripped from testis and stored during the same period of 24 hrs at 4°C shows a significant decrease in the percentage of motile cells from 82 ± 14 to 59 ± 30 (P<0.01) and in the ATP content from 6.15 ± 2.4 to 1.95 ± 1.37 nmoles/108 spermatozoa (P=0.001).
Url:
DOI: 10.1111/j.1439-0426.1999.tb00234.x
Links to Exploration step
ISTEX:EAFE77E21C98BF153F6DFDF82C91DA66638A40D8Le document en format XML
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Fierville</name><affiliation><mods:affiliation>Muséum National d'Histoire Naturelle, Laboratoire d'Ichtyologie, 43, rue Cuvier 75231 Paris Cedex 05, France</mods:affiliation></affiliation></author><author><name sortKey="Brun, R" sort="Brun, R" uniqKey="Brun R" first="R." last="Brun">R. Brun</name><affiliation><mods:affiliation>Cemagref Bordeaux, 50 avenue de Verdun Gazinet 33612 Cestas, France</mods:affiliation></affiliation></author><author><name sortKey="Rouault, T" sort="Rouault, T" uniqKey="Rouault T" first="T." last="Rouault">T. Rouault</name><affiliation><mods:affiliation>Cemagref Bordeaux, 50 avenue de Verdun Gazinet 33612 Cestas, France</mods:affiliation></affiliation></author><author><name sortKey="Williot, P" sort="Williot, P" uniqKey="Williot P" first="P." last="Williot">P. 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About 90% spermatozoa are activated within a few seconds after a double step dilution (final dilution 1/600) and the number of motile cells declines regularly to 10–20% after 2 mn. During the same period of time, the flagellum beat frequency remains stable at about 50 Hz for 30 sec and drops to 30 Hz after 60 sec. A morphological analysis of the movement of the flagellum shows that during the first 20 sec, waves are distributed on the entire length of the flagellum; later, they disappear progressively from the distal part within 1 mn in the case of 90% of the spermatozoa. Finally after 2 mn, most of the flagella are straight and devoid of wave. The ATP content declines very rapidly after activation from 7.58 ± 1.85 to 4.05 ± 1.51 nmol/108 spermatozoa within 5 sec (P=0.004). Spermatozoa left in the genital tract of the males over a period of 24 hrs (in vivo survival) do not show significant change in the percentage of motile cells (82 ± 14 vs 74 ± 15) nor in the ATP content (6.13 ± 2.37 vs 4.59 ± 1.42 nmoles/108 spermatozoa). An aliquot of these spermatozoa stripped from testis and stored during the same period of 24 hrs at 4°C shows a significant decrease in the percentage of motile cells from 82 ± 14 to 59 ± 30 (P<0.01) and in the ATP content from 6.15 ± 2.4 to 1.95 ± 1.37 nmoles/108 spermatozoa (P=0.001).</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>R. Billard</name><affiliations><json:string>Muséum National d'Histoire Naturelle, Laboratoire d'Ichtyologie, 43, rue Cuvier 75231 Paris Cedex 05, France</json:string></affiliations></json:item><json:item><name>J. 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A morphological analysis of the movement of the flagellum shows that during the first 20 sec, waves are distributed on the entire length of the flagellum; later, they disappear progressively from the distal part within 1 mn in the case of 90% of the spermatozoa. Finally after 2 mn, most of the flagella are straight and devoid of wave. The ATP content declines very rapidly after activation from 7.58 ± 1.85 to 4.05 ± 1.51 nmol/108 spermatozoa within 5 sec (P=0.004). Spermatozoa left in the genital tract of the males over a period of 24 hrs (in vivo survival) do not show significant change in the percentage of motile cells (82 ± 14 vs 74 ± 15) nor in the ATP content (6.13 ± 2.37 vs 4.59 ± 1.42 nmoles/108 spermatozoa). An aliquot of these spermatozoa stripped from testis and stored during the same period of 24 hrs at 4°C shows a significant decrease in the percentage of motile cells from 82 ± 14 to 59 ± 30 (P>0.01) and in the ATP content from 6.15 ± 2.4 to 1.95 ± 1.37 nmoles/108 spermatozoa (P=0.001).</abstract><qualityIndicators><score>6.411</score><pdfVersion>1.3</pdfVersion><pdfPageSize>612 x 791.759 pts</pdfPageSize><refBibsNative>true</refBibsNative><abstractCharCount>1514</abstractCharCount><pdfWordCount>3411</pdfWordCount><pdfCharCount>19692</pdfCharCount><pdfPageCount>5</pdfPageCount><abstractWordCount>270</abstractWordCount></qualityIndicators><title>Motility analysis and energetics of the Siberian sturgeon Acipenser baerii 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About 90% spermatozoa are activated within a few seconds after a double step dilution (final dilution 1/600) and the number of motile cells declines regularly to 10–20% after 2 mn. During the same period of time, the flagellum beat frequency remains stable at about 50 Hz for 30 sec and drops to 30 Hz after 60 sec. A morphological analysis of the movement of the flagellum shows that during the first 20 sec, waves are distributed on the entire length of the flagellum; later, they disappear progressively from the distal part within 1 mn in the case of 90% of the spermatozoa. Finally after 2 mn, most of the flagella are straight and devoid of wave. The ATP content declines very rapidly after activation from 7.58 ± 1.85 to 4.05 ± 1.51 nmol/108 spermatozoa within 5 sec (P=0.004). Spermatozoa left in the genital tract of the males over a period of 24 hrs (in vivo survival) do not show significant change in the percentage of motile cells (82 ± 14 vs 74 ± 15) nor in the ATP content (6.13 ± 2.37 vs 4.59 ± 1.42 nmoles/108 spermatozoa). An aliquot of these spermatozoa stripped from testis and stored during the same period of 24 hrs at 4°C shows a significant decrease in the percentage of motile cells from 82 ± 14 to 59 ± 30 (P<0.01) and in the ATP content from 6.15 ± 2.4 to 1.95 ± 1.37 nmoles/108 spermatozoa (P=0.001).</p></abstract></profileDesc><revisionDesc><change when="1999-09">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/EAFE77E21C98BF153F6DFDF82C91DA66638A40D8/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>Blackwell Publishing Ltd</publisherName><publisherLoc>Oxford, UK</publisherLoc></publisherInfo><doi origin="wiley" registered="yes">10.1111/(ISSN)1439-0426</doi><issn type="print">0175-8659</issn><issn type="electronic">1439-0426</issn><idGroup><id type="product" value="JAI"></id><id type="publisherDivision" value="ST"></id></idGroup><titleGroup><title type="main" sort="JOURNAL OF APPLIED ICHTHYOLOGY">Journal of Applied Ichthyology</title></titleGroup></publicationMeta><publicationMeta level="part" position="09004"><doi origin="wiley">10.1111/jai.1999.15.issue-4-5</doi><numberingGroup><numbering type="journalVolume" number="15">15</numbering><numbering type="journalIssue">4‐5</numbering></numberingGroup><coverDate startDate="1999-09">September 1999</coverDate></publicationMeta><publicationMeta level="unit" type="article" position="0019900" status="forIssue"><doi origin="wiley">10.1111/j.1439-0426.1999.tb00234.x</doi><idGroup><id type="unit" value="JAI199"></id></idGroup><countGroup><count type="pageTotal" number="5"></count></countGroup><titleGroup><title type="tocHeading1">Original Article</title></titleGroup><eventGroup><event type="firstOnline" date="2007-07-26"></event><event type="publishedOnlineFinalForm" date="2007-07-26"></event><event type="xmlConverted" agent="Converter:BPG_TO_WML3G version:2.3.5 mode:FullText source:HeaderRef result:HeaderRef" date="2010-04-07"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:3.8.8" date="2014-01-28"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-10-23"></event></eventGroup><numberingGroup><numbering type="pageFirst" number="199">199</numbering><numbering type="pageLast" number="203">203</numbering></numberingGroup><linkGroup><link type="toTypesetVersion" href="file:JAI.JAI199.pdf"></link></linkGroup></publicationMeta><contentMeta><countGroup><count type="referenceTotal" number="21"></count><count type="linksCrossRef" number="6"></count></countGroup><titleGroup><title type="main">Motility analysis and energetics of the Siberian sturgeon Acipenser baerii spermatozoa</title></titleGroup><creators><creator creatorRole="author" xml:id="cr1" affiliationRef="#a1"><personName><givenNames>R.</givenNames><familyName>Billard</familyName></personName></creator><creator creatorRole="author" xml:id="cr2" affiliationRef="#a2"><personName><givenNames>J.</givenNames><familyName>Cosson</familyName></personName></creator><creator creatorRole="author" xml:id="cr3" affiliationRef="#a1"><personName><givenNames>F.</givenNames><familyName>Fierville</familyName></personName></creator><creator creatorRole="author" xml:id="cr4" affiliationRef="#a3"><personName><givenNames>R.</givenNames><familyName>Brun</familyName></personName></creator><creator creatorRole="author" xml:id="cr5" affiliationRef="#a3"><personName><givenNames>T.</givenNames><familyName>Rouault</familyName></personName></creator><creator creatorRole="author" xml:id="cr6" affiliationRef="#a3"><personName><givenNames>P.</givenNames><familyName>Williot</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="a1" countryCode="FR"><unparsedAffiliation>Muséum National d'Histoire Naturelle, Laboratoire d'Ichtyologie, 43, rue Cuvier 75231 Paris Cedex 05, France</unparsedAffiliation></affiliation><affiliation xml:id="a2" countryCode="FR"><unparsedAffiliation>CNRS URA 671 Station Zoologique 06230 Villefranche sur Mer, France</unparsedAffiliation></affiliation><affiliation xml:id="a3" countryCode="FR"><unparsedAffiliation>Cemagref Bordeaux, 50 avenue de Verdun Gazinet 33612 Cestas, France</unparsedAffiliation></affiliation></affiliationGroup><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Summary</title><p>A study of motility was carried out on spermatozoa of the Siberian sturgeon (<i>Acipenser baerii</i> Brandt) reared at the Cemagref experimental research station, St Seurin sur l'Isle, France. About 90% spermatozoa are activated within a few seconds after a double step dilution (final dilution 1/600) and the number of motile cells declines regularly to 10–20% after 2 mn. During the same period of time, the flagellum beat frequency remains stable at about 50 Hz for 30 sec and drops to 30 Hz after 60 sec. A morphological analysis of the movement of the flagellum shows that during the first 20 sec, waves are distributed on the entire length of the flagellum; later, they disappear progressively from the distal part within 1 mn in the case of 90% of the spermatozoa. Finally after 2 mn, most of the flagella are straight and devoid of wave. The ATP content declines very rapidly after activation from 7.58 ± 1.85 to 4.05 ± 1.51 nmol/108 spermatozoa within 5 sec (P=0.004). Spermatozoa left in the genital tract of the males over a period of 24 hrs (in vivo survival) do not show significant change in the percentage of motile cells (82 ± 14 vs 74 ± 15) nor in the ATP content (6.13 ± 2.37 vs 4.59 ± 1.42 nmoles/108 spermatozoa). An aliquot of these spermatozoa stripped from testis and stored during the same period of 24 hrs at 4°C shows a significant decrease in the percentage of motile cells from 82 ± 14 to 59 ± 30 (P<0.01) and in the ATP content from 6.15 ± 2.4 to 1.95 ± 1.37 nmoles/108 spermatozoa (P=0.001).</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Motility analysis and energetics of the Siberian sturgeon Acipenser baerii spermatozoa</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Motility analysis and energetics of the Siberian sturgeon Acipenser baerii spermatozoa</title></titleInfo><name type="personal"><namePart type="given">R.</namePart><namePart type="family">Billard</namePart><affiliation>Muséum National d'Histoire Naturelle, Laboratoire d'Ichtyologie, 43, rue Cuvier 75231 Paris Cedex 05, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J.</namePart><namePart type="family">Cosson</namePart><affiliation>CNRS URA 671 Station Zoologique 06230 Villefranche sur Mer, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">F.</namePart><namePart type="family">Fierville</namePart><affiliation>Muséum National d'Histoire Naturelle, Laboratoire d'Ichtyologie, 43, rue Cuvier 75231 Paris Cedex 05, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">R.</namePart><namePart type="family">Brun</namePart><affiliation>Cemagref Bordeaux, 50 avenue de Verdun Gazinet 33612 Cestas, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">T.</namePart><namePart type="family">Rouault</namePart><affiliation>Cemagref Bordeaux, 50 avenue de Verdun Gazinet 33612 Cestas, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">P.</namePart><namePart type="family">Williot</namePart><affiliation>Cemagref Bordeaux, 50 avenue de Verdun Gazinet 33612 Cestas, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Blackwell Publishing Ltd</publisher><place><placeTerm type="text">Oxford, UK</placeTerm></place><dateIssued encoding="w3cdtf">1999-09</dateIssued><copyrightDate encoding="w3cdtf">1999</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="references">21</extent></physicalDescription><abstract lang="en">A study of motility was carried out on spermatozoa of the Siberian sturgeon (Acipenser baerii Brandt) reared at the Cemagref experimental research station, St Seurin sur l'Isle, France. About 90% spermatozoa are activated within a few seconds after a double step dilution (final dilution 1/600) and the number of motile cells declines regularly to 10–20% after 2 mn. During the same period of time, the flagellum beat frequency remains stable at about 50 Hz for 30 sec and drops to 30 Hz after 60 sec. A morphological analysis of the movement of the flagellum shows that during the first 20 sec, waves are distributed on the entire length of the flagellum; later, they disappear progressively from the distal part within 1 mn in the case of 90% of the spermatozoa. Finally after 2 mn, most of the flagella are straight and devoid of wave. The ATP content declines very rapidly after activation from 7.58 ± 1.85 to 4.05 ± 1.51 nmol/108 spermatozoa within 5 sec (P=0.004). Spermatozoa left in the genital tract of the males over a period of 24 hrs (in vivo survival) do not show significant change in the percentage of motile cells (82 ± 14 vs 74 ± 15) nor in the ATP content (6.13 ± 2.37 vs 4.59 ± 1.42 nmoles/108 spermatozoa). An aliquot of these spermatozoa stripped from testis and stored during the same period of 24 hrs at 4°C shows a significant decrease in the percentage of motile cells from 82 ± 14 to 59 ± 30 (P<0.01) and in the ATP content from 6.15 ± 2.4 to 1.95 ± 1.37 nmoles/108 spermatozoa (P=0.001).</abstract><relatedItem type="host"><titleInfo><title>Journal of Applied Ichthyology</title></titleInfo><genre type="journal">journal</genre><identifier type="ISSN">0175-8659</identifier><identifier type="eISSN">1439-0426</identifier><identifier type="DOI">10.1111/(ISSN)1439-0426</identifier><identifier type="PublisherID">JAI</identifier><part><date>1999</date><detail type="volume"><caption>vol.</caption><number>15</number></detail><detail type="issue"><caption>no.</caption><number>4‐5</number></detail><extent unit="pages"><start>199</start><end>203</end><total>5</total></extent></part></relatedItem><identifier type="istex">EAFE77E21C98BF153F6DFDF82C91DA66638A40D8</identifier><identifier type="DOI">10.1111/j.1439-0426.1999.tb00234.x</identifier><identifier type="ArticleID">JAI199</identifier><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>Blackwell Publishing Ltd</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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