Sperm density, seminal plasma composition and their physiological relationship in the endangered Caspian brown trout (Salmo trutta caspius)
Identifieur interne : 000A15 ( Istex/Corpus ); précédent : 000A14; suivant : 000A16Sperm density, seminal plasma composition and their physiological relationship in the endangered Caspian brown trout (Salmo trutta caspius)
Auteurs : Azadeh Hatef ; Hamid Niksirat ; Bagher Mojazi Amiri ; Sayed Mohammad Hadi Alavi ; Mahmoud KaramiSource :
- Aquaculture Research [ 1355-557X ] ; 2007-08.
English descriptors
Abstract
Sperm density, mineral and organic composition of the seminal plasma and their physiological relationship were investigated in the Caspian brown trout (Salmo trutta caspius). To establish a rapid and accurate method for assessment of sperm density, three different techniques were used: sperm counting, spectrophotometry (at 480 nm) and determination of the spermatocrit. The seminal plasma contained 159.26±8.84 mM sodium (Na), 33.72±2.01 mM potassium (K), 133.04±5.96 mM chlorine (Cl), 1.68±0.2 mM calcium (Ca) and 0.988±0.13 mM magnesium (Mg). The following organic components were found: total protein 0.75±0.14 mg 100 mL−1, cholesterol 2.86±0.58 mg L−1 and glucose 3.81±1.04 mM L−1. The mean sperm density was estimated to be 3.3 × 109 spermatozoa mL−1. The spermatocrit (%) ranged from 25 to 52 in sperm samples. Highly significant linear relationships were found between sperm density and spermatocrit (R2=0.703, P<0.001) and sperm density and optical density (R2=0.909, P<0.001), indicating that optical density can be used as a quick and accurate method of estimating sperm density. Significant relationships were also found between sperm density and Ca, Mg and total protein of seminal plasma. A significant correlation was also observed between the Ca and Mg concentrations (R2=0.774, P<0.01). The following correlations were observed between mineral and organic components: total protein and Ca (R2=0.462, P<0.05), total protein and Mg (R2=0.518, P<0.05) and glucose and Cl (R2=0.374, P<0.05). These parameters should be considered when developing procedures for either artificial fertilization or for cryopreservation of sperm.
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DOI: 10.1111/j.1365-2109.2007.01784.x
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To establish a rapid and accurate method for assessment of sperm density, three different techniques were used: sperm counting, spectrophotometry (at 480 nm) and determination of the spermatocrit. The seminal plasma contained 159.26±8.84 mM sodium (Na), 33.72±2.01 mM potassium (K), 133.04±5.96 mM chlorine (Cl), 1.68±0.2 mM calcium (Ca) and 0.988±0.13 mM magnesium (Mg). The following organic components were found: total protein 0.75±0.14 mg 100 mL−1, cholesterol 2.86±0.58 mg L−1 and glucose 3.81±1.04 mM L−1. The mean sperm density was estimated to be 3.3 × 109 spermatozoa mL−1. The spermatocrit (%) ranged from 25 to 52 in sperm samples. Highly significant linear relationships were found between sperm density and spermatocrit (R2=0.703, P<0.001) and sperm density and optical density (R2=0.909, P<0.001), indicating that optical density can be used as a quick and accurate method of estimating sperm density. Significant relationships were also found between sperm density and Ca, Mg and total protein of seminal plasma. A significant correlation was also observed between the Ca and Mg concentrations (R2=0.774, P<0.01). The following correlations were observed between mineral and organic components: total protein and Ca (R2=0.462, P<0.05), total protein and Mg (R2=0.518, P<0.05) and glucose and Cl (R2=0.374, P<0.05). 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To establish a rapid and accurate method for assessment of sperm density, three different techniques were used: sperm counting, spectrophotometry (at 480 nm) and determination of the spermatocrit. The seminal plasma contained 159.26±8.84 mM sodium (Na), 33.72±2.01 mM potassium (K), 133.04±5.96 mM chlorine (Cl), 1.68±0.2 mM calcium (Ca) and 0.988±0.13 mM magnesium (Mg). The following organic components were found: total protein 0.75±0.14 mg 100 mL−1, cholesterol 2.86±0.58 mg L−1 and glucose 3.81±1.04 mM L−1. The mean sperm density was estimated to be 3.3 × 109 spermatozoa mL−1. The spermatocrit (%) ranged from 25 to 52 in sperm samples. Highly significant linear relationships were found between sperm density and spermatocrit (R2=0.703, P>0.001) and sperm density and optical density (R2=0.909, P>0.001), indicating that optical density can be used as a quick and accurate method of estimating sperm density. Significant relationships were also found between sperm density and Ca, Mg and total protein of seminal plasma. A significant correlation was also observed between the Ca and Mg concentrations (R2=0.774, P>0.01). The following correlations were observed between mineral and organic components: total protein and Ca (R2=0.462, P>0.05), total protein and Mg (R2=0.518, P>0.05) and glucose and Cl (R2=0.374, P>0.05). 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To establish a rapid and accurate method for assessment of sperm density, three different techniques were used: sperm counting, spectrophotometry (at 480 nm) and determination of the spermatocrit. The seminal plasma contained 159.26±8.84 mM sodium (Na), 33.72±2.01 mM potassium (K), 133.04±5.96 mM chlorine (Cl), 1.68±0.2 mM calcium (Ca) and 0.988±0.13 mM magnesium (Mg). The following organic components were found: total protein 0.75±0.14 mg 100 mL−1, cholesterol 2.86±0.58 mg L−1 and glucose 3.81±1.04 mM L−1. The mean sperm density was estimated to be 3.3 × 109 spermatozoa mL−1. The spermatocrit (%) ranged from 25 to 52 in sperm samples. Highly significant linear relationships were found between sperm density and spermatocrit (R2=0.703, P<0.001) and sperm density and optical density (R2=0.909, P<0.001), indicating that optical density can be used as a quick and accurate method of estimating sperm density. Significant relationships were also found between sperm density and Ca, Mg and total protein of seminal plasma. A significant correlation was also observed between the Ca and Mg concentrations (R2=0.774, P<0.01). The following correlations were observed between mineral and organic components: total protein and Ca (R2=0.462, P<0.05), total protein and Mg (R2=0.518, P<0.05) and glucose and Cl (R2=0.374, P<0.05). These parameters should be considered when developing procedures for either artificial fertilization or for cryopreservation of sperm.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>keywords</head><item><term>sperm density</term></item><item><term>seminal plasma</term></item><item><term>Caspian brown trout</term></item><item><term>mineral and organic components</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2007-08">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/6786B1158DB04194B36DB20A80D82CF9CA4464D6/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>Blackwell Publishing Ltd</publisherName><publisherLoc>Oxford, UK</publisherLoc></publisherInfo><doi origin="wiley" registered="yes">10.1111/(ISSN)1365-2109</doi><issn type="print">1355-557X</issn><issn type="electronic">1365-2109</issn><idGroup><id type="product" value="ARE"></id><id type="publisherDivision" value="ST"></id></idGroup><titleGroup><title type="main" sort="AQUACULTURE RESEARCH">Aquaculture Research</title></titleGroup></publicationMeta><publicationMeta level="part" position="08011"><doi origin="wiley">10.1111/are.2007.38.issue-11</doi><numberingGroup><numbering type="journalVolume" number="38">38</numbering><numbering type="journalIssue" number="11">11</numbering></numberingGroup><coverDate startDate="2007-08">August 2007</coverDate></publicationMeta><publicationMeta level="unit" type="article" position="8" status="forIssue"><doi origin="wiley">10.1111/j.1365-2109.2007.01784.x</doi><idGroup><id type="unit" value="ARE1784"></id><id type="supplier" value="1784"></id></idGroup><countGroup><count type="pageTotal" number="7"></count></countGroup><titleGroup><title type="tocHeading1">Original articles</title></titleGroup><eventGroup><event type="firstOnline" date="2007-07-10"></event><event type="publishedOnlineFinalForm" date="2007-08-01"></event><event type="xmlConverted" agent="Converter:BPG_TO_WML3G version:2.3.4 mode:FullText source:FullText result:FullText" date="2010-03-30"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:3.8.8" date="2014-01-06"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-10-15"></event></eventGroup><numberingGroup><numbering type="pageFirst" number="1175">1175</numbering><numbering type="pageLast" number="1181">1181</numbering></numberingGroup><correspondenceTo><b>Correspondence:</b> A Hatef, Department of Fisheries and Environmental Sciences, Faculty of Natural Resources, University of Tehran, 31585‐4314 Karaj, Iran. E‐mail: <email normalForm="a_hatef171@yahoo.com">a_hatef171@yahoo.com</email></correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:ARE.ARE1784.pdf"></link></linkGroup></publicationMeta><contentMeta><countGroup><count type="figureTotal" number="3"></count><count type="tableTotal" number="1"></count><count type="formulaTotal" number="0"></count><count type="referenceTotal" number="36"></count><count type="wordTotal" number="4447"></count><count type="linksCrossRef" number="57"></count></countGroup><titleGroup><title type="main">Sperm density, seminal plasma composition and their physiological relationship in the endangered Caspian brown trout (<i>Salmo trutta caspius</i>)</title><title type="shortAuthors">A Hatef <i>et al.</i></title><title type="short">Sperm density and seminal plasma composition in <i>Salmo trutta caspius</i></title></titleGroup><creators><creator creatorRole="author" xml:id="cr1" affiliationRef="#a1"><personName><givenNames>Azadeh</givenNames><familyName>Hatef</familyName></personName></creator><creator creatorRole="author" xml:id="cr2" affiliationRef="#a1"><personName><givenNames>Hamid</givenNames><familyName>Niksirat</familyName></personName></creator><creator creatorRole="author" xml:id="cr3" affiliationRef="#a1"><personName><givenNames>Bagher Mojazi</givenNames><familyName>Amiri</familyName></personName></creator><creator creatorRole="author" xml:id="cr4" affiliationRef="#a2"><personName><givenNames>Sayed Mohammad Hadi</givenNames><familyName>Alavi</familyName></personName></creator><creator creatorRole="author" xml:id="cr5" affiliationRef="#a1"><personName><givenNames>Mahmoud</givenNames><familyName>Karami</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="a1"><unparsedAffiliation>Department of Fisheries and Environmental Sciences, Faculty of Natural Resources, University of Tehran, Karaj, Iran</unparsedAffiliation></affiliation><affiliation xml:id="a2" countryCode="CZ"><unparsedAffiliation>Research Institute of Fish Culture and Hydrobiology, University of South Bohemia, Vodnany, Czech Republic</unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en"><keyword xml:id="k1">sperm density</keyword><keyword xml:id="k2">seminal plasma</keyword><keyword xml:id="k3">Caspian brown trout</keyword><keyword xml:id="k4">mineral and organic components</keyword></keywordGroup><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Abstract</title><p>Sperm density, mineral and organic composition of the seminal plasma and their physiological relationship were investigated in the Caspian brown trout (<i>Salmo trutta caspius</i>). To establish a rapid and accurate method for assessment of sperm density, three different techniques were used: sperm counting, spectrophotometry (at 480 nm) and determination of the spermatocrit. The seminal plasma contained 159.26±8.84 mM sodium (Na), 33.72±2.01 mM potassium (K), 133.04±5.96 mM chlorine (Cl), 1.68±0.2 mM calcium (Ca) and 0.988±0.13 mM magnesium (Mg). The following organic components were found: total protein 0.75±0.14 mg 100 mL<sup>−1</sup>, cholesterol 2.86±0.58 mg L<sup>−1</sup> and glucose 3.81±1.04 mM L<sup>−1</sup>. The mean sperm density was estimated to be 3.3 × 10<sup>9</sup> spermatozoa mL<sup>−1</sup>. The spermatocrit (%) ranged from 25 to 52 in sperm samples. Highly significant linear relationships were found between sperm density and spermatocrit (<i>R</i><sup>2</sup>=0.703, <i>P</i><0.001) and sperm density and optical density (<i>R</i><sup>2</sup>=0.909, <i>P</i><0.001), indicating that optical density can be used as a quick and accurate method of estimating sperm density. Significant relationships were also found between sperm density and Ca, Mg and total protein of seminal plasma. A significant correlation was also observed between the Ca and Mg concentrations (<i>R</i><sup>2</sup>=0.774, <i>P</i><0.01). The following correlations were observed between mineral and organic components: total protein and Ca (<i>R</i><sup>2</sup>=0.462, <i>P</i><0.05), total protein and Mg (<i>R</i><sup>2</sup>=0.518, <i>P</i><0.05) and glucose and Cl (<i>R</i><sup>2</sup>=0.374, <i>P</i><0.05). These parameters should be considered when developing procedures for either artificial fertilization or for cryopreservation of sperm.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Sperm density, seminal plasma composition and their physiological relationship in the endangered Caspian brown trout (Salmo trutta caspius)</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>Sperm density and seminal plasma composition in Salmo trutta caspius</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Sperm density, seminal plasma composition and their physiological relationship in the endangered Caspian brown trout (Salmo trutta caspius)</title></titleInfo><name type="personal"><namePart type="given">Azadeh</namePart><namePart type="family">Hatef</namePart><affiliation>Department of Fisheries and Environmental Sciences, Faculty of Natural Resources, University of Tehran, Karaj, Iran</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Hamid</namePart><namePart type="family">Niksirat</namePart><affiliation>Department of Fisheries and Environmental Sciences, Faculty of Natural Resources, University of Tehran, Karaj, Iran</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Bagher Mojazi</namePart><namePart type="family">Amiri</namePart><affiliation>Department of Fisheries and Environmental Sciences, Faculty of Natural Resources, University of Tehran, Karaj, Iran</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Sayed Mohammad Hadi</namePart><namePart type="family">Alavi</namePart><affiliation>Research Institute of Fish Culture and Hydrobiology, University of South Bohemia, Vodnany, Czech Republic</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Mahmoud</namePart><namePart type="family">Karami</namePart><affiliation>Department of Fisheries and Environmental Sciences, Faculty of Natural Resources, University of Tehran, Karaj, Iran</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Blackwell Publishing Ltd</publisher><place><placeTerm type="text">Oxford, UK</placeTerm></place><dateIssued encoding="w3cdtf">2007-08</dateIssued><copyrightDate encoding="w3cdtf">2007</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">3</extent><extent unit="tables">1</extent><extent unit="references">36</extent><extent unit="words">4447</extent></physicalDescription><abstract lang="en">Sperm density, mineral and organic composition of the seminal plasma and their physiological relationship were investigated in the Caspian brown trout (Salmo trutta caspius). To establish a rapid and accurate method for assessment of sperm density, three different techniques were used: sperm counting, spectrophotometry (at 480 nm) and determination of the spermatocrit. The seminal plasma contained 159.26±8.84 mM sodium (Na), 33.72±2.01 mM potassium (K), 133.04±5.96 mM chlorine (Cl), 1.68±0.2 mM calcium (Ca) and 0.988±0.13 mM magnesium (Mg). The following organic components were found: total protein 0.75±0.14 mg 100 mL−1, cholesterol 2.86±0.58 mg L−1 and glucose 3.81±1.04 mM L−1. The mean sperm density was estimated to be 3.3 × 109 spermatozoa mL−1. The spermatocrit (%) ranged from 25 to 52 in sperm samples. Highly significant linear relationships were found between sperm density and spermatocrit (R2=0.703, P<0.001) and sperm density and optical density (R2=0.909, P<0.001), indicating that optical density can be used as a quick and accurate method of estimating sperm density. Significant relationships were also found between sperm density and Ca, Mg and total protein of seminal plasma. A significant correlation was also observed between the Ca and Mg concentrations (R2=0.774, P<0.01). The following correlations were observed between mineral and organic components: total protein and Ca (R2=0.462, P<0.05), total protein and Mg (R2=0.518, P<0.05) and glucose and Cl (R2=0.374, P<0.05). These parameters should be considered when developing procedures for either artificial fertilization or for cryopreservation of sperm.</abstract><subject lang="en"><genre>keywords</genre><topic>sperm density</topic><topic>seminal plasma</topic><topic>Caspian brown trout</topic><topic>mineral and organic components</topic></subject><relatedItem type="host"><titleInfo><title>Aquaculture Research</title></titleInfo><genre type="journal">journal</genre><identifier type="ISSN">1355-557X</identifier><identifier type="eISSN">1365-2109</identifier><identifier type="DOI">10.1111/(ISSN)1365-2109</identifier><identifier type="PublisherID">ARE</identifier><part><date>2007</date><detail type="volume"><caption>vol.</caption><number>38</number></detail><detail type="issue"><caption>no.</caption><number>11</number></detail><extent unit="pages"><start>1175</start><end>1181</end><total>7</total></extent></part></relatedItem><identifier type="istex">6786B1158DB04194B36DB20A80D82CF9CA4464D6</identifier><identifier type="DOI">10.1111/j.1365-2109.2007.01784.x</identifier><identifier type="ArticleID">ARE1784</identifier><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>Blackwell Publishing Ltd</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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