Sperm motility and seminal plasma characteristics in Barbus sharpeyi (Günther, 1874)
Identifieur interne : 000A09 ( Istex/Corpus ); précédent : 000A08; suivant : 000A10Sperm motility and seminal plasma characteristics in Barbus sharpeyi (Günther, 1874)
Auteurs : Sayyed Mohammad Hadi Alavi ; Elham Jorfi ; Azadeh Hatef ; Sayyed Abdul Saheb MortezaviSource :
- Aquaculture Research [ 1355-557X ] ; 2010-09.
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- KwdEn :
Abstract
Spermatozoa concentration, ionic composition, osmolality, glucose and total protein contents of seminal plasma and sperm motility were determined in Barbus sharpeyi (Cyprinidae, Teleosotei). Spermatozoa concentration ranged from 9.77 to 20.20 × 109 spermatozoa mL−1. Osmolality (mOsmol kg−1) and ionic contents (mM L−1) of the seminal plasma were 274.5±9.0, 70.0±3.4 Na+, 28.8±0.9 K+, 101.7±3.1 Cl−, 0.9±0.1 Mg2+ and 2.1±0.1 Ca2+ respectively. Total protein and glucose were 5.3±0.2 g L−1 and 76.7±4.3 mM L−1 respectively. Sperm motility was initiated in a hypo‐osmotic condition, composed of either an ionic (KCl or NaCl) or a non‐ionic (sucrose) activation medium. Duration of sperm motility was very short: <2 min after activation in distilled water. Percentage of motile spermatozoa was significantly higher in an activation medium containing NaCl compared with that of distilled water. An activating medium containing NaCl or KCl higher than 150 mM or sucrose higher than 275 mM totally inhibited the activation of sperm motility. Immediately after sperm activation, wave(s) propagated along the flagellum, but waves were restricted to the proximal part of the flagellum (close to the head) at 1 min post activation. Studied characteristics in the present study were compared with those of other cyprinids for understanding inter‐species differences.
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DOI: 10.1111/j.1365-2109.2010.02600.x
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ISTEX:4B20AC2D9111E0D0D6F942F3950B7F067480B1A5Le document en format XML
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Spermatozoa concentration ranged from 9.77 to 20.20 × 109 spermatozoa mL−1. Osmolality (mOsmol kg−1) and ionic contents (mM L−1) of the seminal plasma were 274.5±9.0, 70.0±3.4 Na+, 28.8±0.9 K+, 101.7±3.1 Cl−, 0.9±0.1 Mg2+ and 2.1±0.1 Ca2+ respectively. Total protein and glucose were 5.3±0.2 g L−1 and 76.7±4.3 mM L−1 respectively. Sperm motility was initiated in a hypo‐osmotic condition, composed of either an ionic (KCl or NaCl) or a non‐ionic (sucrose) activation medium. Duration of sperm motility was very short: <2 min after activation in distilled water. Percentage of motile spermatozoa was significantly higher in an activation medium containing NaCl compared with that of distilled water. An activating medium containing NaCl or KCl higher than 150 mM or sucrose higher than 275 mM totally inhibited the activation of sperm motility. Immediately after sperm activation, wave(s) propagated along the flagellum, but waves were restricted to the proximal part of the flagellum (close to the head) at 1 min post activation. Studied characteristics in the present study were compared with those of other cyprinids for understanding inter‐species differences.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>Sayyed Mohammad Hadi Alavi</name><affiliations><json:string>Research Institute of Fish Culture and Hydrobiology, Faculty of Fisheries and Protection of Waters, University of South Bohemia, Vodnany, Czech Republic</json:string></affiliations></json:item><json:item><name>Elham Jorfi</name><affiliations><json:string>Department of Genetics and Aquaculture, South of Iran Aquaculture Research Center, Ahwaz, Iran</json:string></affiliations></json:item><json:item><name>Azadeh Hatef</name><affiliations><json:string>Research Institute of Fish Culture and Hydrobiology, Faculty of Fisheries and Protection of Waters, University of South Bohemia, Vodnany, Czech Republic</json:string></affiliations></json:item><json:item><name>Sayyed Abdul Saheb Mortezavi</name><affiliations><json:string>Department of Genetics and Aquaculture, South of Iran Aquaculture Research Center, Ahwaz, Iran</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>sperm motility</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>ions</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>osmolality</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>flagella</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>spermatozoa concentration</value></json:item></subject><articleId><json:string>ARE2600</json:string></articleId><language><json:string>eng</json:string></language><originalGenre><json:string>article</json:string></originalGenre><abstract>Spermatozoa concentration, ionic composition, osmolality, glucose and total protein contents of seminal plasma and sperm motility were determined in Barbus sharpeyi (Cyprinidae, Teleosotei). 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Immediately after sperm activation, wave(s) propagated along the flagellum, but waves were restricted to the proximal part of the flagellum (close to the head) at 1 min post activation. 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Spermatozoa concentration ranged from 9.77 to 20.20 × 109 spermatozoa mL−1. Osmolality (mOsmol kg−1) and ionic contents (mM L−1) of the seminal plasma were 274.5±9.0, 70.0±3.4 Na+, 28.8±0.9 K+, 101.7±3.1 Cl−, 0.9±0.1 Mg2+ and 2.1±0.1 Ca2+ respectively. Total protein and glucose were 5.3±0.2 g L−1 and 76.7±4.3 mM L−1 respectively. Sperm motility was initiated in a hypo‐osmotic condition, composed of either an ionic (KCl or NaCl) or a non‐ionic (sucrose) activation medium. Duration of sperm motility was very short: <2 min after activation in distilled water. Percentage of motile spermatozoa was significantly higher in an activation medium containing NaCl compared with that of distilled water. An activating medium containing NaCl or KCl higher than 150 mM or sucrose higher than 275 mM totally inhibited the activation of sperm motility. Immediately after sperm activation, wave(s) propagated along the flagellum, but waves were restricted to the proximal part of the flagellum (close to the head) at 1 min post activation. Studied characteristics in the present study were compared with those of other cyprinids for understanding inter‐species differences.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>keywords</head><item><term>sperm motility</term></item><item><term>ions</term></item><item><term>osmolality</term></item><item><term>flagella</term></item><item><term>spermatozoa concentration</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2010-09">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/4B20AC2D9111E0D0D6F942F3950B7F067480B1A5/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>Blackwell Publishing Ltd</publisherName><publisherLoc>Oxford, UK</publisherLoc></publisherInfo><doi origin="wiley" registered="yes">10.1111/(ISSN)1365-2109</doi><issn type="print">1355-557X</issn><issn type="electronic">1365-2109</issn><idGroup><id type="product" value="ARE"></id><id type="publisherDivision" value="ST"></id></idGroup><titleGroup><title type="main" sort="AQUACULTURE RESEARCH">Aquaculture Research</title></titleGroup></publicationMeta><publicationMeta level="part" position="09110"><doi origin="wiley">10.1111/are.2010.41.issue-10</doi><numberingGroup><numbering type="journalVolume" number="41">41</numbering><numbering type="journalIssue" number="10">10</numbering></numberingGroup><coverDate startDate="2010-09">September 2010</coverDate></publicationMeta><publicationMeta level="unit" type="article" position="50" status="forIssue"><doi origin="wiley">10.1111/j.1365-2109.2010.02600.x</doi><idGroup><id type="unit" value="ARE2600"></id><id type="supplier" value="2600"></id></idGroup><countGroup><count type="pageTotal" number="7"></count></countGroup><titleGroup><title type="tocHeading1">Original Articles</title></titleGroup><copyright>© 2010 The Authors. Aquaculture Research © 2010 Blackwell Publishing Ltd</copyright><eventGroup><event type="firstOnline" date="2010-07-22"></event><event type="publishedOnlineAcceptedOrEarlyUnpaginated" date="2010-07-22"></event><event type="xmlConverted" agent="Converter:BPG_TO_WML3G version:2.3.25 mode:FullText source:FullText result:FullText" date="2010-11-05"></event><event type="publishedOnlineFinalForm" date="2010-07-22"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:3.8.8" date="2014-01-06"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-10-15"></event></eventGroup><numberingGroup><numbering type="pageFirst">e688</numbering><numbering type="pageLast">e694</numbering></numberingGroup><correspondenceTo><b>Correspondence:</b> S M H Alavi, Research Institute of Fish Culture and Hydrobiology, Faculty of Fisheries and Protection of Waters, University of South Bohemia, Vodnany 389 25, Czech Republic. E‐mail: <email normalForm="alavi@vurh.jcu.cz">alavi@vurh.jcu.cz</email></correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:ARE.ARE2600.pdf"></link></linkGroup></publicationMeta><contentMeta><countGroup><count type="figureTotal" number="4"></count><count type="tableTotal" number="1"></count><count type="formulaTotal" number="0"></count><count type="referenceTotal" number="45"></count><count type="wordTotal" number="5153"></count><count type="linksCrossRef" number="63"></count></countGroup><titleGroup><title type="main">Sperm motility and seminal plasma characteristics in <i>Barbus sharpeyi</i> (Günther, 1874)</title><title type="shortAuthors">S M Hadi Alavi <i>et al.</i></title><title type="short"><i>Barbus sharpeyi</i> sperm</title></titleGroup><creators><creator creatorRole="author" xml:id="cr1" affiliationRef="#a1"><personName><givenNames>Sayyed Mohammad</givenNames><familyName>Hadi Alavi</familyName></personName></creator><creator creatorRole="author" xml:id="cr2" affiliationRef="#a2"><personName><givenNames>Elham</givenNames><familyName>Jorfi</familyName></personName></creator><creator creatorRole="author" xml:id="cr3" affiliationRef="#a1"><personName><givenNames>Azadeh</givenNames><familyName>Hatef</familyName></personName></creator><creator creatorRole="author" xml:id="cr4" affiliationRef="#a2"><personName><givenNames>Sayyed Abdul</givenNames><familyName>Saheb Mortezavi</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="a1" countryCode="CZ"><unparsedAffiliation>Research Institute of Fish Culture and Hydrobiology, Faculty of Fisheries and Protection of Waters, University of South Bohemia, Vodnany, Czech Republic</unparsedAffiliation></affiliation><affiliation xml:id="a2"><unparsedAffiliation>Department of Genetics and Aquaculture, South of Iran Aquaculture Research Center, Ahwaz, Iran</unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en"><keyword xml:id="k1">sperm motility</keyword><keyword xml:id="k2">ions</keyword><keyword xml:id="k3">osmolality</keyword><keyword xml:id="k4">flagella</keyword><keyword xml:id="k5">spermatozoa concentration</keyword></keywordGroup><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Abstract</title><p>Spermatozoa concentration, ionic composition, osmolality, glucose and total protein contents of seminal plasma and sperm motility were determined in <i>Barbus sharpeyi</i> (Cyprinidae, Teleosotei). Spermatozoa concentration ranged from 9.77 to 20.20 × 10<sup>9</sup> spermatozoa mL<sup>−1</sup>. Osmolality (mOsmol kg<sup>−1</sup>) and ionic contents (mM L<sup>−1</sup>) of the seminal plasma were 274.5±9.0, 70.0±3.4 Na<sup>+</sup>, 28.8±0.9 K<sup>+</sup>, 101.7±3.1 Cl<sup>−</sup>, 0.9±0.1 Mg<sup>2+</sup> and 2.1±0.1 Ca<sup>2+</sup> respectively. Total protein and glucose were 5.3±0.2 g L<sup>−1</sup> and 76.7±4.3 mM L<sup>−1</sup> respectively. Sperm motility was initiated in a hypo‐osmotic condition, composed of either an ionic (KCl or NaCl) or a non‐ionic (sucrose) activation medium. Duration of sperm motility was very short: <2 min after activation in distilled water. Percentage of motile spermatozoa was significantly higher in an activation medium containing NaCl compared with that of distilled water. An activating medium containing NaCl or KCl higher than 150 mM or sucrose higher than 275 mM totally inhibited the activation of sperm motility. Immediately after sperm activation, wave(s) propagated along the flagellum, but waves were restricted to the proximal part of the flagellum (close to the head) at 1 min post activation. Studied characteristics in the present study were compared with those of other cyprinids for understanding inter‐species differences.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Sperm motility and seminal plasma characteristics in Barbus sharpeyi (Günther, 1874)</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>Barbus sharpeyi sperm</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Sperm motility and seminal plasma characteristics in Barbus sharpeyi (Günther, 1874)</title></titleInfo><name type="personal"><namePart type="given">Sayyed Mohammad</namePart><namePart type="family">Hadi Alavi</namePart><affiliation>Research Institute of Fish Culture and Hydrobiology, Faculty of Fisheries and Protection of Waters, University of South Bohemia, Vodnany, Czech Republic</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Elham</namePart><namePart type="family">Jorfi</namePart><affiliation>Department of Genetics and Aquaculture, South of Iran Aquaculture Research Center, Ahwaz, Iran</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Azadeh</namePart><namePart type="family">Hatef</namePart><affiliation>Research Institute of Fish Culture and Hydrobiology, Faculty of Fisheries and Protection of Waters, University of South Bohemia, Vodnany, Czech Republic</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Sayyed Abdul</namePart><namePart type="family">Saheb Mortezavi</namePart><affiliation>Department of Genetics and Aquaculture, South of Iran Aquaculture Research Center, Ahwaz, Iran</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Blackwell Publishing Ltd</publisher><place><placeTerm type="text">Oxford, UK</placeTerm></place><dateIssued encoding="w3cdtf">2010-09</dateIssued><copyrightDate encoding="w3cdtf">2010</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">4</extent><extent unit="tables">1</extent><extent unit="references">45</extent><extent unit="words">5153</extent></physicalDescription><abstract lang="en">Spermatozoa concentration, ionic composition, osmolality, glucose and total protein contents of seminal plasma and sperm motility were determined in Barbus sharpeyi (Cyprinidae, Teleosotei). Spermatozoa concentration ranged from 9.77 to 20.20 × 109 spermatozoa mL−1. Osmolality (mOsmol kg−1) and ionic contents (mM L−1) of the seminal plasma were 274.5±9.0, 70.0±3.4 Na+, 28.8±0.9 K+, 101.7±3.1 Cl−, 0.9±0.1 Mg2+ and 2.1±0.1 Ca2+ respectively. Total protein and glucose were 5.3±0.2 g L−1 and 76.7±4.3 mM L−1 respectively. Sperm motility was initiated in a hypo‐osmotic condition, composed of either an ionic (KCl or NaCl) or a non‐ionic (sucrose) activation medium. Duration of sperm motility was very short: <2 min after activation in distilled water. Percentage of motile spermatozoa was significantly higher in an activation medium containing NaCl compared with that of distilled water. An activating medium containing NaCl or KCl higher than 150 mM or sucrose higher than 275 mM totally inhibited the activation of sperm motility. Immediately after sperm activation, wave(s) propagated along the flagellum, but waves were restricted to the proximal part of the flagellum (close to the head) at 1 min post activation. Studied characteristics in the present study were compared with those of other cyprinids for understanding inter‐species differences.</abstract><subject lang="en"><genre>keywords</genre><topic>sperm motility</topic><topic>ions</topic><topic>osmolality</topic><topic>flagella</topic><topic>spermatozoa concentration</topic></subject><relatedItem type="host"><titleInfo><title>Aquaculture Research</title></titleInfo><genre type="journal">journal</genre><identifier type="ISSN">1355-557X</identifier><identifier type="eISSN">1365-2109</identifier><identifier type="DOI">10.1111/(ISSN)1365-2109</identifier><identifier type="PublisherID">ARE</identifier><part><date>2010</date><detail type="volume"><caption>vol.</caption><number>41</number></detail><detail type="issue"><caption>no.</caption><number>10</number></detail><extent unit="pages"><start>e688</start><end>e694</end><total>7</total></extent></part></relatedItem><identifier type="istex">4B20AC2D9111E0D0D6F942F3950B7F067480B1A5</identifier><identifier type="DOI">10.1111/j.1365-2109.2010.02600.x</identifier><identifier type="ArticleID">ARE2600</identifier><accessCondition type="use and reproduction" contentType="copyright">© 2010 The Authors. 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