Cryopreservation of paddlefish sperm in 5‐mL straws
Identifieur interne : 000964 ( Istex/Corpus ); précédent : 000963; suivant : 000965Cryopreservation of paddlefish sperm in 5‐mL straws
Auteurs : Á. Horváth ; B. Urbányi ; C. Wang ; R. J. Onders ; S. D. MimsSource :
- Journal of Applied Ichthyology [ 0175-8659 ] ; 2010-10.
Abstract
Experiments were conducted to test the feasibility of using 5‐mL straws for the cryopreservation of paddlefish (Polyodon spathula) sperm. In experiment 1, the effects of 5% or 10% methanol as a cryoprotectant in combination with cooling times of 5 or 7 min on paddlefish sperm stored in 5‐mL straws were evaluated for fertilization and hatching rates. Highest fertilization rate of 48 ± 5% (mean ± SE) and hatching rate of 47 ± 10% were observed using sperm cryopreserved with 5% methanol and a 5‐min cooling time in liquid nitrogen vapors. However, fertilization and hatching rates were significantly lower with cryopreserved sperm than with fresh sperm (fertilization 77 ± 6%; hatching 66 ± 13%). In experiment 2, the effects of sperm : egg ratios on fertilization rates were investigated. When fresh sperm was used, fertilization rate was quadratically related to sperm : egg ratio (y = −13.19x2 + 55.90x + 38.44, r2 = 0.823) and the optimum range of sperm : egg ratios was between 1.379 × 106 and 2.758 × 106. When sperm were cooled for 5 min with 5% methanol, fertilization rate was linearly related to sperm : egg ratio (y = 22.51x + 23.26, r2 = 0.75) but optimum sperm : egg ratio was not reached. In experiment 3, the hatching rates were not significantly different between the three‐straw treatment and the control. With cryopreserved sperm, the relationship between the sperm and egg ratios and the hatching rates were best described by a quadratic equation (y = −29.65x2 + 119.2x − 51.04, r2 = 0.837). Therefore, when cryopreserved sperm is used, sperm : egg ratio should be increased significantly to optimize fertilization and hatching rates. This can be achieved either by increasing the total volume of cryopreserved sperm by at least 30% or by further research to improve the procedure to increase the viability of post‐thawed sperm per straw.
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DOI: 10.1111/j.1439-0426.2010.01551.x
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<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Cryopreservation of paddlefish sperm in 5‐mL straws</title><author><name sortKey="Horvath, A" sort="Horvath, A" uniqKey="Horvath A" first="Á." last="Horváth">Á. Horváth</name><affiliation><mods:affiliation>Szent István University, Páter K. u. 1., Gödöllő, Hungary</mods:affiliation></affiliation></author><author><name sortKey="Urbanyi, B" sort="Urbanyi, B" uniqKey="Urbanyi B" first="B." last="Urbányi">B. Urbányi</name><affiliation><mods:affiliation>Szent István University, Páter K. u. 1., Gödöllő, Hungary</mods:affiliation></affiliation></author><author><name sortKey="Wang, C" sort="Wang, C" uniqKey="Wang C" first="C." last="Wang">C. Wang</name><affiliation><mods:affiliation>Aquaculture Research Center, Kentucky State University, Frankfort, Kentucky, USA</mods:affiliation></affiliation></author><author><name sortKey="Onders, R J" sort="Onders, R J" uniqKey="Onders R" first="R. J." last="Onders">R. J. Onders</name><affiliation><mods:affiliation>Aquaculture Research Center, Kentucky State University, Frankfort, Kentucky, USA</mods:affiliation></affiliation></author><author><name sortKey="Mims, S D" sort="Mims, S D" uniqKey="Mims S" first="S. D." last="Mims">S. D. Mims</name><affiliation><mods:affiliation>Aquaculture Research Center, Kentucky State University, Frankfort, Kentucky, USA</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:321DCAB1B124E8D7F86706BF69429C0A0269627B</idno><date when="2010" year="2010">2010</date><idno type="doi">10.1111/j.1439-0426.2010.01551.x</idno><idno type="url">https://api.istex.fr/document/321DCAB1B124E8D7F86706BF69429C0A0269627B/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000964</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000964</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Cryopreservation of paddlefish sperm in 5‐mL straws</title><author><name sortKey="Horvath, A" sort="Horvath, A" uniqKey="Horvath A" first="Á." last="Horváth">Á. Horváth</name><affiliation><mods:affiliation>Szent István University, Páter K. u. 1., Gödöllő, Hungary</mods:affiliation></affiliation></author><author><name sortKey="Urbanyi, B" sort="Urbanyi, B" uniqKey="Urbanyi B" first="B." last="Urbányi">B. Urbányi</name><affiliation><mods:affiliation>Szent István University, Páter K. u. 1., Gödöllő, Hungary</mods:affiliation></affiliation></author><author><name sortKey="Wang, C" sort="Wang, C" uniqKey="Wang C" first="C." last="Wang">C. Wang</name><affiliation><mods:affiliation>Aquaculture Research Center, Kentucky State University, Frankfort, Kentucky, USA</mods:affiliation></affiliation></author><author><name sortKey="Onders, R J" sort="Onders, R J" uniqKey="Onders R" first="R. J." last="Onders">R. J. Onders</name><affiliation><mods:affiliation>Aquaculture Research Center, Kentucky State University, Frankfort, Kentucky, USA</mods:affiliation></affiliation></author><author><name sortKey="Mims, S D" sort="Mims, S D" uniqKey="Mims S" first="S. D." last="Mims">S. D. Mims</name><affiliation><mods:affiliation>Aquaculture Research Center, Kentucky State University, Frankfort, Kentucky, USA</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Applied Ichthyology</title><idno type="ISSN">0175-8659</idno><idno type="eISSN">1439-0426</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2010-10">2010-10</date><biblScope unit="volume">26</biblScope><biblScope unit="issue">5</biblScope><biblScope unit="page" from="715">715</biblScope><biblScope unit="page" to="719">719</biblScope></imprint><idno type="ISSN">0175-8659</idno></series><idno type="istex">321DCAB1B124E8D7F86706BF69429C0A0269627B</idno><idno type="DOI">10.1111/j.1439-0426.2010.01551.x</idno><idno type="ArticleID">JAI1551</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0175-8659</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Experiments were conducted to test the feasibility of using 5‐mL straws for the cryopreservation of paddlefish (Polyodon spathula) sperm. In experiment 1, the effects of 5% or 10% methanol as a cryoprotectant in combination with cooling times of 5 or 7 min on paddlefish sperm stored in 5‐mL straws were evaluated for fertilization and hatching rates. Highest fertilization rate of 48 ± 5% (mean ± SE) and hatching rate of 47 ± 10% were observed using sperm cryopreserved with 5% methanol and a 5‐min cooling time in liquid nitrogen vapors. However, fertilization and hatching rates were significantly lower with cryopreserved sperm than with fresh sperm (fertilization 77 ± 6%; hatching 66 ± 13%). In experiment 2, the effects of sperm : egg ratios on fertilization rates were investigated. When fresh sperm was used, fertilization rate was quadratically related to sperm : egg ratio (y = −13.19x2 + 55.90x + 38.44, r2 = 0.823) and the optimum range of sperm : egg ratios was between 1.379 × 106 and 2.758 × 106. When sperm were cooled for 5 min with 5% methanol, fertilization rate was linearly related to sperm : egg ratio (y = 22.51x + 23.26, r2 = 0.75) but optimum sperm : egg ratio was not reached. In experiment 3, the hatching rates were not significantly different between the three‐straw treatment and the control. With cryopreserved sperm, the relationship between the sperm and egg ratios and the hatching rates were best described by a quadratic equation (y = −29.65x2 + 119.2x − 51.04, r2 = 0.837). Therefore, when cryopreserved sperm is used, sperm : egg ratio should be increased significantly to optimize fertilization and hatching rates. This can be achieved either by increasing the total volume of cryopreserved sperm by at least 30% or by further research to improve the procedure to increase the viability of post‐thawed sperm per straw.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>Á. Horváth</name><affiliations><json:string>Szent István University, Páter K. u. 1., Gödöllő, Hungary</json:string></affiliations></json:item><json:item><name>B. Urbányi</name><affiliations><json:string>Szent István University, Páter K. u. 1., Gödöllő, Hungary</json:string></affiliations></json:item><json:item><name>C. Wang</name><affiliations><json:string>Aquaculture Research Center, Kentucky State University, Frankfort, Kentucky, USA</json:string></affiliations></json:item><json:item><name>R. J. Onders</name><affiliations><json:string>Aquaculture Research Center, Kentucky State University, Frankfort, Kentucky, USA</json:string></affiliations></json:item><json:item><name>S. D. Mims</name><affiliations><json:string>Aquaculture Research Center, Kentucky State University, Frankfort, Kentucky, USA</json:string></affiliations></json:item></author><articleId><json:string>JAI1551</json:string></articleId><language><json:string>eng</json:string></language><originalGenre><json:string>article</json:string></originalGenre><abstract>Experiments were conducted to test the feasibility of using 5‐mL straws for the cryopreservation of paddlefish (Polyodon spathula) sperm. In experiment 1, the effects of 5% or 10% methanol as a cryoprotectant in combination with cooling times of 5 or 7 min on paddlefish sperm stored in 5‐mL straws were evaluated for fertilization and hatching rates. Highest fertilization rate of 48 ± 5% (mean ± SE) and hatching rate of 47 ± 10% were observed using sperm cryopreserved with 5% methanol and a 5‐min cooling time in liquid nitrogen vapors. However, fertilization and hatching rates were significantly lower with cryopreserved sperm than with fresh sperm (fertilization 77 ± 6%; hatching 66 ± 13%). In experiment 2, the effects of sperm : egg ratios on fertilization rates were investigated. When fresh sperm was used, fertilization rate was quadratically related to sperm : egg ratio (y = −13.19x2 + 55.90x + 38.44, r2 = 0.823) and the optimum range of sperm : egg ratios was between 1.379 × 106 and 2.758 × 106. When sperm were cooled for 5 min with 5% methanol, fertilization rate was linearly related to sperm : egg ratio (y = 22.51x + 23.26, r2 = 0.75) but optimum sperm : egg ratio was not reached. In experiment 3, the hatching rates were not significantly different between the three‐straw treatment and the control. With cryopreserved sperm, the relationship between the sperm and egg ratios and the hatching rates were best described by a quadratic equation (y = −29.65x2 + 119.2x − 51.04, r2 = 0.837). Therefore, when cryopreserved sperm is used, sperm : egg ratio should be increased significantly to optimize fertilization and hatching rates. 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J.</forename><surname>Onders</surname></persName><affiliation>Aquaculture Research Center, Kentucky State University, Frankfort, Kentucky, USA</affiliation></author><author xml:id="author-5"><persName><forename type="first">S. D.</forename><surname>Mims</surname></persName><affiliation>Aquaculture Research Center, Kentucky State University, Frankfort, Kentucky, USA</affiliation></author></analytic><monogr><title level="j">Journal of Applied Ichthyology</title><idno type="pISSN">0175-8659</idno><idno type="eISSN">1439-0426</idno><idno type="DOI">10.1111/(ISSN)1439-0426</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2010-10"></date><biblScope unit="volume">26</biblScope><biblScope unit="issue">5</biblScope><biblScope unit="page" from="715">715</biblScope><biblScope unit="page" to="719">719</biblScope></imprint></monogr><idno type="istex">321DCAB1B124E8D7F86706BF69429C0A0269627B</idno><idno type="DOI">10.1111/j.1439-0426.2010.01551.x</idno><idno type="ArticleID">JAI1551</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2010</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Experiments were conducted to test the feasibility of using 5‐mL straws for the cryopreservation of paddlefish (Polyodon spathula) sperm. In experiment 1, the effects of 5% or 10% methanol as a cryoprotectant in combination with cooling times of 5 or 7 min on paddlefish sperm stored in 5‐mL straws were evaluated for fertilization and hatching rates. Highest fertilization rate of 48 ± 5% (mean ± SE) and hatching rate of 47 ± 10% were observed using sperm cryopreserved with 5% methanol and a 5‐min cooling time in liquid nitrogen vapors. However, fertilization and hatching rates were significantly lower with cryopreserved sperm than with fresh sperm (fertilization 77 ± 6%; hatching 66 ± 13%). In experiment 2, the effects of sperm : egg ratios on fertilization rates were investigated. When fresh sperm was used, fertilization rate was quadratically related to sperm : egg ratio (y = −13.19x2 + 55.90x + 38.44, r2 = 0.823) and the optimum range of sperm : egg ratios was between 1.379 × 106 and 2.758 × 106. When sperm were cooled for 5 min with 5% methanol, fertilization rate was linearly related to sperm : egg ratio (y = 22.51x + 23.26, r2 = 0.75) but optimum sperm : egg ratio was not reached. In experiment 3, the hatching rates were not significantly different between the three‐straw treatment and the control. With cryopreserved sperm, the relationship between the sperm and egg ratios and the hatching rates were best described by a quadratic equation (y = −29.65x2 + 119.2x − 51.04, r2 = 0.837). Therefore, when cryopreserved sperm is used, sperm : egg ratio should be increased significantly to optimize fertilization and hatching rates. This can be achieved either by increasing the total volume of cryopreserved sperm by at least 30% or by further research to improve the procedure to increase the viability of post‐thawed sperm per straw.</p></abstract></profileDesc><revisionDesc><change when="2010-10">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/321DCAB1B124E8D7F86706BF69429C0A0269627B/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>Blackwell Publishing Ltd</publisherName><publisherLoc>Oxford, UK</publisherLoc></publisherInfo><doi origin="wiley" registered="yes">10.1111/(ISSN)1439-0426</doi><issn type="print">0175-8659</issn><issn type="electronic">1439-0426</issn><idGroup><id type="product" value="JAI"></id><id type="publisherDivision" value="ST"></id></idGroup><titleGroup><title type="main" sort="JOURNAL OF APPLIED ICHTHYOLOGY">Journal of Applied Ichthyology</title></titleGroup></publicationMeta><publicationMeta level="part" position="10105"><doi origin="wiley">10.1111/jai.2010.26.issue-5</doi><titleGroup><title type="specialIssueTitle">Proceedings of the Second International Workshop on Biology of Fish Gametes
Special Issue Editors: Harald Rosenthal, Juan F. Asturiano, Otomar Linhart, Akos Horvath</title></titleGroup><numberingGroup><numbering type="journalVolume" number="26">26</numbering><numbering type="journalIssue">5</numbering></numberingGroup><coverDate startDate="2010-10">October 2010</coverDate></publicationMeta><publicationMeta level="unit" type="article" position="16" status="forIssue"><doi origin="wiley">10.1111/j.1439-0426.2010.01551.x</doi><idGroup><id type="unit" value="JAI1551"></id></idGroup><countGroup><count type="pageTotal" number="5"></count></countGroup><titleGroup><title type="tocHeading1">Original articles</title></titleGroup><copyright>© 2010 Blackwell Verlag, Berlin</copyright><eventGroup><event type="xmlConverted" agent="Converter:BPG_TO_WML3G version:2.3.19 mode:FullText" date="2010-09-17"></event><event type="firstOnline" date="2010-09-17"></event><event type="publishedOnlineFinalForm" date="2010-09-17"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:3.8.8" date="2014-01-28"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-10-23"></event></eventGroup><numberingGroup><numbering type="pageFirst" number="715">715</numbering><numbering type="pageLast" number="719">719</numbering></numberingGroup><correspondenceTo><b>Author’s address:</b>Ákos Horváth, Szent István University, Páter K. u. 1., Gödöllő, H‐2103, Hungary.
E‐mail: <email>Horvath.Akos@mkk.szie.hu</email></correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:JAI.JAI1551.pdf"></link></linkGroup></publicationMeta><contentMeta><unparsedEditorialHistory>Received: March 14, 2010 Accepted: July 30, 2010</unparsedEditorialHistory><countGroup><count type="figureTotal" number="4"></count><count type="tableTotal" number="1"></count></countGroup><titleGroup><title type="main">Cryopreservation of paddlefish sperm in 5‐mL straws</title><title type="shortAuthors">Á. Horváth et al.</title><title type="short">Paddlefish sperm cryopreservation</title></titleGroup><creators><creator creatorRole="author" xml:id="cr1" affiliationRef="#a1"><personName><givenNames>Á.</givenNames><familyName>Horváth</familyName></personName></creator><creator creatorRole="author" xml:id="cr2" affiliationRef="#a1"><personName><givenNames>B.</givenNames><familyName>Urbányi</familyName></personName></creator><creator creatorRole="author" xml:id="cr3" affiliationRef="#a2"><personName><givenNames>C.</givenNames><familyName>Wang</familyName></personName></creator><creator creatorRole="author" xml:id="cr4" affiliationRef="#a2"><personName><givenNames>R. J.</givenNames><familyName>Onders</familyName></personName></creator><creator creatorRole="author" xml:id="cr5" affiliationRef="#a2"><personName><givenNames>S. D.</givenNames><familyName>Mims</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="a1" countryCode="HU"><unparsedAffiliation>Szent István University, Páter K. u. 1., Gödöllő, Hungary</unparsedAffiliation></affiliation><affiliation xml:id="a2" countryCode="US"><unparsedAffiliation>Aquaculture Research Center, Kentucky State University, Frankfort, Kentucky, USA</unparsedAffiliation></affiliation></affiliationGroup><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Summary</title><p>Experiments were conducted to test the feasibility of using 5‐mL straws for the cryopreservation of paddlefish (<i>Polyodon spathula</i>) sperm. In experiment 1, the effects of 5% or 10% methanol as a cryoprotectant in combination with cooling times of 5 or 7 min on paddlefish sperm stored in 5‐mL straws were evaluated for fertilization and hatching rates. Highest fertilization rate of 48 ± 5% (mean ± SE) and hatching rate of 47 ± 10% were observed using sperm cryopreserved with 5% methanol and a 5‐min cooling time in liquid nitrogen vapors. However, fertilization and hatching rates were significantly lower with cryopreserved sperm than with fresh sperm (fertilization 77 ± 6%; hatching 66 ± 13%). In experiment 2, the effects of sperm : egg ratios on fertilization rates were investigated. When fresh sperm was used, fertilization rate was quadratically related to sperm : egg ratio (<i>y</i> = −13.19<i>x</i><sup>2</sup> + 55.90<i>x</i> + 38.44, <i>r</i><sup>2</sup> = 0.823) and the optimum range of sperm : egg ratios was between 1.379 × 10<sup>6</sup> and 2.758 × 10<sup>6</sup>. When sperm were cooled for 5 min with 5% methanol, fertilization rate was linearly related to sperm : egg ratio (<i>y</i> = 22.51<i>x</i> + 23.26, <i>r</i><sup>2</sup> = 0.75) but optimum sperm : egg ratio was not reached. In experiment 3, the hatching rates were not significantly different between the three‐straw treatment and the control. With cryopreserved sperm, the relationship between the sperm and egg ratios and the hatching rates were best described by a quadratic equation (<i>y</i> = −29.65<i>x</i><sup>2</sup> + 119.2<i>x</i> − 51.04, <i>r</i><sup>2</sup> = 0.837). Therefore, when cryopreserved sperm is used, sperm : egg ratio should be increased significantly to optimize fertilization and hatching rates. This can be achieved either by increasing the total volume of cryopreserved sperm by at least 30% or by further research to improve the procedure to increase the viability of post‐thawed sperm per straw.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Cryopreservation of paddlefish sperm in 5‐mL straws</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>Paddlefish sperm cryopreservation</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Cryopreservation of paddlefish sperm in 5‐mL straws</title></titleInfo><name type="personal"><namePart type="given">Á.</namePart><namePart type="family">Horváth</namePart><affiliation>Szent István University, Páter K. u. 1., Gödöllő, Hungary</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">B.</namePart><namePart type="family">Urbányi</namePart><affiliation>Szent István University, Páter K. u. 1., Gödöllő, Hungary</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">C.</namePart><namePart type="family">Wang</namePart><affiliation>Aquaculture Research Center, Kentucky State University, Frankfort, Kentucky, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">R. J.</namePart><namePart type="family">Onders</namePart><affiliation>Aquaculture Research Center, Kentucky State University, Frankfort, Kentucky, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S. D.</namePart><namePart type="family">Mims</namePart><affiliation>Aquaculture Research Center, Kentucky State University, Frankfort, Kentucky, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Blackwell Publishing Ltd</publisher><place><placeTerm type="text">Oxford, UK</placeTerm></place><dateIssued encoding="w3cdtf">2010-10</dateIssued><edition>Received: March 14, 2010 Accepted: July 30, 2010</edition><copyrightDate encoding="w3cdtf">2010</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">4</extent><extent unit="tables">1</extent></physicalDescription><abstract lang="en">Experiments were conducted to test the feasibility of using 5‐mL straws for the cryopreservation of paddlefish (Polyodon spathula) sperm. In experiment 1, the effects of 5% or 10% methanol as a cryoprotectant in combination with cooling times of 5 or 7 min on paddlefish sperm stored in 5‐mL straws were evaluated for fertilization and hatching rates. Highest fertilization rate of 48 ± 5% (mean ± SE) and hatching rate of 47 ± 10% were observed using sperm cryopreserved with 5% methanol and a 5‐min cooling time in liquid nitrogen vapors. However, fertilization and hatching rates were significantly lower with cryopreserved sperm than with fresh sperm (fertilization 77 ± 6%; hatching 66 ± 13%). In experiment 2, the effects of sperm : egg ratios on fertilization rates were investigated. When fresh sperm was used, fertilization rate was quadratically related to sperm : egg ratio (y = −13.19x2 + 55.90x + 38.44, r2 = 0.823) and the optimum range of sperm : egg ratios was between 1.379 × 106 and 2.758 × 106. When sperm were cooled for 5 min with 5% methanol, fertilization rate was linearly related to sperm : egg ratio (y = 22.51x + 23.26, r2 = 0.75) but optimum sperm : egg ratio was not reached. In experiment 3, the hatching rates were not significantly different between the three‐straw treatment and the control. With cryopreserved sperm, the relationship between the sperm and egg ratios and the hatching rates were best described by a quadratic equation (y = −29.65x2 + 119.2x − 51.04, r2 = 0.837). Therefore, when cryopreserved sperm is used, sperm : egg ratio should be increased significantly to optimize fertilization and hatching rates. This can be achieved either by increasing the total volume of cryopreserved sperm by at least 30% or by further research to improve the procedure to increase the viability of post‐thawed sperm per straw.</abstract><relatedItem type="host"><titleInfo><title>Journal of Applied Ichthyology</title></titleInfo><genre type="journal">journal</genre><identifier type="ISSN">0175-8659</identifier><identifier type="eISSN">1439-0426</identifier><identifier type="DOI">10.1111/(ISSN)1439-0426</identifier><identifier type="PublisherID">JAI</identifier><part><date>2010</date><detail type="title"><title>Proceedings of the Second International Workshop on Biology of Fish Gametes Special Issue Editors: Harald Rosenthal, Juan F. Asturiano, Otomar Linhart, Akos Horvath</title></detail><detail type="volume"><caption>vol.</caption><number>26</number></detail><detail type="issue"><caption>no.</caption><number>5</number></detail><extent unit="pages"><start>715</start><end>719</end><total>5</total></extent></part></relatedItem><identifier type="istex">321DCAB1B124E8D7F86706BF69429C0A0269627B</identifier><identifier type="DOI">10.1111/j.1439-0426.2010.01551.x</identifier><identifier type="ArticleID">JAI1551</identifier><accessCondition type="use and reproduction" contentType="copyright">© 2010 Blackwell Verlag, Berlin</accessCondition><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>Blackwell Publishing Ltd</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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