Expression of Agouti‐related Protein (Agrp) and its mRNA in the Hypothalamus and the Adrenal Gland of the Duck (Anas platyrhynchos)
Identifieur interne : 000957 ( Istex/Corpus ); précédent : 000956; suivant : 000958Expression of Agouti‐related Protein (Agrp) and its mRNA in the Hypothalamus and the Adrenal Gland of the Duck (Anas platyrhynchos)
Auteurs : N. Mirabella ; V. Esposito ; C. Squillacioti ; A. De Luca ; G. Germano ; G. PainoSource :
- Anatomia, Histologia, Embryologia [ 0340-2096 ] ; 2005-12.
Abstract
Introduction: Agouti‐related protein (AGRP) is a neuropeptide involved in the control of body weight. Morphological and pharmacological studies have shown that AGRP is implicated in the central control of feeding behaviour acting as an endogenous antagonist of the alpha‐melanocyte stimulating hormone (α‐MSH), a potent satiety‐inducing factor, at the melanocortin 3 (MC3)‐ and four (MC4)‐receptors.
Url:
DOI: 10.1111/j.1439-0264.2005.00669_75.x
Links to Exploration step
ISTEX:42FFFC7EA512C2C04659B542CC5A894BED79C14CLe document en format XML
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Paino</name><affiliation><mods:affiliation>Department of structures, functions and biological technologies, Faculty of Veterinary Medicine, University of Naples Federico II, 80137, Naples, Italy</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Anatomia, Histologia, Embryologia</title><idno type="ISSN">0340-2096</idno><idno type="eISSN">1439-0264</idno><imprint><publisher>Blackwell Verlag GmbH</publisher><pubPlace>Berlin, Germany</pubPlace><date type="published" when="2005-12">2005-12</date><biblScope unit="volume">34</biblScope><biblScope unit="supplement">s1</biblScope><biblScope unit="page" from="33">33</biblScope><biblScope unit="page" to="33">33</biblScope></imprint><idno type="ISSN">0340-2096</idno></series><idno type="istex">42FFFC7EA512C2C04659B542CC5A894BED79C14C</idno><idno type="DOI">10.1111/j.1439-0264.2005.00669_75.x</idno><idno type="ArticleID">AHE669_75_75</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0340-2096</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract">Introduction: Agouti‐related protein (AGRP) is a neuropeptide involved in the control of body weight. Morphological and pharmacological studies have shown that AGRP is implicated in the central control of feeding behaviour acting as an endogenous antagonist of the alpha‐melanocyte stimulating hormone (α‐MSH), a potent satiety‐inducing factor, at the melanocortin 3 (MC3)‐ and four (MC4)‐receptors.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>N. Mirabella</name><affiliations><json:string>Department of structures, functions and biological technologies, Faculty of Veterinary Medicine, University of Naples Federico II, 80137, Naples, Italy</json:string></affiliations></json:item><json:item><name>V. Esposito</name><affiliations><json:string>Department of structures, functions and biological technologies, Faculty of Veterinary Medicine, University of Naples Federico II, 80137, Naples, Italy</json:string></affiliations></json:item><json:item><name>C. 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platyrhynchos)</title><respStmt><resp>Références bibliographiques récupérées via GROBID</resp><name resp="ISTEX-API">ISTEX-API (INIST-CNRS)</name></respStmt><respStmt><resp>Références bibliographiques récupérées via GROBID</resp><name resp="ISTEX-API">ISTEX-API (INIST-CNRS)</name></respStmt></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>Blackwell Verlag GmbH</publisher><pubPlace>Berlin, Germany</pubPlace><availability><p>WILEY</p></availability><date>2005</date></publicationStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Expression of Agouti‐related Protein (Agrp) and its mRNA in the Hypothalamus and the Adrenal Gland of the Duck (Anas platyrhynchos)</title><author xml:id="author-1"><persName><forename type="first">N.</forename><surname>Mirabella</surname></persName><affiliation>Department of structures, functions and biological technologies, Faculty of Veterinary Medicine, University of Naples Federico II, 80137, Naples, Italy</affiliation></author><author xml:id="author-2"><persName><forename type="first">V.</forename><surname>Esposito</surname></persName><affiliation>Department of structures, functions and biological technologies, Faculty of Veterinary Medicine, University of Naples Federico II, 80137, Naples, Italy</affiliation></author><author xml:id="author-3"><persName><forename type="first">C.</forename><surname>Squillacioti</surname></persName><affiliation>Department of structures, functions and biological technologies, Faculty of Veterinary Medicine, University of Naples Federico II, 80137, Naples, Italy</affiliation></author><author xml:id="author-4"><persName><forename type="first">A.</forename><surname>De Luca</surname></persName><affiliation>Department of structures, functions and biological technologies, Faculty of Veterinary Medicine, University of Naples Federico II, 80137, Naples, Italy</affiliation></author><author xml:id="author-5"><persName><forename type="first">G.</forename><surname>Germano</surname></persName><affiliation>Department of structures, functions and biological technologies, Faculty of Veterinary Medicine, University of Naples Federico II, 80137, Naples, Italy</affiliation></author><author xml:id="author-6"><persName><forename type="first">G.</forename><surname>Paino</surname></persName><affiliation>Department of structures, functions and biological technologies, Faculty of Veterinary Medicine, University of Naples Federico II, 80137, Naples, Italy</affiliation></author></analytic><monogr><title level="j">Anatomia, Histologia, Embryologia</title><idno type="pISSN">0340-2096</idno><idno type="eISSN">1439-0264</idno><idno type="DOI">10.1111/(ISSN)1439-0264</idno><imprint><publisher>Blackwell Verlag GmbH</publisher><pubPlace>Berlin, Germany</pubPlace><date type="published" when="2005-12"></date><biblScope unit="volume">34</biblScope><biblScope unit="supplement">s1</biblScope><biblScope unit="page" 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Morphological and pharmacological studies have shown that AGRP is implicated in the central control of feeding behaviour acting as an endogenous antagonist of the alpha‐melanocyte stimulating hormone (α‐MSH), a potent satiety‐inducing factor, at the melanocortin 3 (MC3)‐ and four (MC4)‐receptors.</p></abstract><abstract><p>Aim: The aim of the present study was to investigate the expression of AGRP and its mRNA in the hypothalamus and adrenal gland of the duck and, in particular, to establish which type of adrenal tissue is involved in the AGRP synthesis.</p></abstract><abstract><p>Methods: Immunohistochemistry, western blotting, reverse transcriptase (RT)‐polimerase chain reaction (PCR).</p></abstract><abstract><p>Results and Discussion: AGRP‐immunoreactivity was observed in neurons and nerve fibres in a restricted area of the hypothalamus. AGRP‐ir neurons were located in the nucleus infundibularis and distributed ventromedially to the third ventricle in the hypothalamic tuberal region. These neurons were round or, with a lesser extent, elongated in shape. AGRP‐ir fibres were seen to project to the median eminence (ME) and anterior periventricular hypothalamus. The AGRP ir‐fibres in the ME were distributed in the external layer in close vicinity to the capillaries of the hypothalamo‐hypophysial portal system. In the avian adrenal gland, AGRP immunoreactivity was observed in the medullary tissue. A partial sequence of AGRP cDNA was identified using RT‐PCR cloning and sequencing. This sequence was highly homologous to the corresponding fragment of the chicken AGRP gene. The western blotting analysis of adrenal gland and hypothalamus tissue extracts showed a well‐defined single band with an electrophoretic mobility consistent with the molecular weight of the avian AGRP protein. These results demonstrate that AGRP is expressed in the hypothalamus and adrenal glands of the duck and suggest an involvement of this peptide in the regulation of the melanocortin system in birds.</p></abstract></profileDesc><revisionDesc><change when="2005-12">Published</change><change xml:id="refBibs-istex" who="#ISTEX-API" when="2016-12-13">References added</change><change xml:id="refBibs-istex" who="#ISTEX-API" when="2017-02-8">References added</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/42FFFC7EA512C2C04659B542CC5A894BED79C14C/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley component found"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>Blackwell Verlag GmbH</publisherName><publisherLoc>Berlin, Germany</publisherLoc></publisherInfo><doi origin="wiley" registered="yes">10.1111/(ISSN)1439-0264</doi><issn type="print">0340-2096</issn><issn type="electronic">1439-0264</issn><idGroup><id type="product" value="AHE"></id><id type="publisherDivision" value="ST"></id></idGroup><titleGroup><title type="main" sort="ANATOMIA HISTOLOGIA EMBRYOLOGIA">Anatomia, Histologia, Embryologia</title></titleGroup></publicationMeta><publicationMeta level="part" position="12000"><doi origin="wiley">10.1111/ahe.2005.34.issue-s1</doi><numberingGroup><numbering type="journalVolume" number="34">34</numbering><numbering type="supplement">s1</numbering></numberingGroup><coverDate startDate="2005-12">December 2005</coverDate></publicationMeta><publicationMeta level="unit" type="abstract" position="1" status="forIssue"><doi origin="wiley">10.1111/j.1439-0264.2005.00669_75.x</doi><idGroup><id type="unit" value="AHE669_75_75"></id></idGroup><titleGroup><title type="tocHeading1">Abstracts of the XXVth Congress of the European Association of Veterinary Anatomists (EAVA), Oslo, 28–31 July 2004</title></titleGroup><eventGroup><event type="firstOnline" date="2005-11-22"></event><event type="publishedOnlineFinalForm" date="2005-11-22"></event><event type="xmlConverted" agent="Converter:BPG_TO_WML3G version:2.3.6 mode:FullText source:Header result:Header" date="2010-04-14"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:3.8.8" date="2014-01-01"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-10-14"></event></eventGroup><numberingGroup><numbering type="pageFirst" number="33">33</numbering><numbering type="pageLast" number="33">33</numbering></numberingGroup><linkGroup><link type="toTypesetVersion" href="file:AHE.AHE669_75_75.pdf"></link></linkGroup></publicationMeta><contentMeta><titleGroup><title type="main">Expression of Agouti‐related Protein (Agrp) and its mRNA in the Hypothalamus and the Adrenal Gland of the Duck (Anas platyrhynchos)</title><title type="short">Abstracts</title></titleGroup><creators><creator creatorRole="author" xml:id="cr1" affiliationRef="#aff-1-1"><personName><givenNames>N.</givenNames><familyName>Mirabella</familyName></personName></creator><creator creatorRole="author" xml:id="cr2" affiliationRef="#aff-1-1"><personName><givenNames>V.</givenNames><familyName>Esposito</familyName></personName></creator><creator creatorRole="author" xml:id="cr3" affiliationRef="#aff-1-1"><personName><givenNames>C.</givenNames><familyName>Squillacioti</familyName></personName></creator><creator creatorRole="author" xml:id="cr4" affiliationRef="#aff-1-1"><personName><givenNames>A.</givenNames><familyName>De Luca</familyName></personName></creator><creator creatorRole="author" xml:id="cr5" affiliationRef="#aff-1-1"><personName><givenNames>G.</givenNames><familyName>Germano</familyName></personName></creator><creator creatorRole="author" xml:id="cr6" affiliationRef="#aff-1-1"><personName><givenNames>G.</givenNames><familyName>Paino</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="aff-1-1" countryCode="IT"><unparsedAffiliation>Department of structures, functions and biological technologies, Faculty of Veterinary Medicine, University of Naples Federico II, 80137, Naples, Italy</unparsedAffiliation></affiliation></affiliationGroup><abstractGroup><abstract type="main" xml:lang="en"><p><b>Introduction: </b> Agouti‐related protein (AGRP) is a neuropeptide involved in the control of body weight. Morphological and pharmacological studies have shown that AGRP is implicated in the central control of feeding behaviour acting as an endogenous antagonist of the alpha‐melanocyte stimulating hormone (α‐MSH), a potent satiety‐inducing factor, at the melanocortin 3 (MC3)‐ and four (MC4)‐receptors.</p><p><b>Aim: </b> The aim of the present study was to investigate the expression of AGRP and its mRNA in the hypothalamus and adrenal gland of the duck and, in particular, to establish which type of adrenal tissue is involved in the AGRP synthesis.</p><p><b>Methods: </b> Immunohistochemistry, western blotting, reverse transcriptase (RT)‐polimerase chain reaction (PCR).</p><p><b>Results and Discussion: </b> AGRP‐immunoreactivity was observed in neurons and nerve fibres in a restricted area of the hypothalamus. AGRP‐ir neurons were located in the nucleus infundibularis and distributed ventromedially to the third ventricle in the hypothalamic tuberal region. These neurons were round or, with a lesser extent, elongated in shape. AGRP‐ir fibres were seen to project to the median eminence (ME) and anterior periventricular hypothalamus. The AGRP ir‐fibres in the ME were distributed in the external layer in close vicinity to the capillaries of the hypothalamo‐hypophysial portal system. In the avian adrenal gland, AGRP immunoreactivity was observed in the medullary tissue. A partial sequence of AGRP cDNA was identified using RT‐PCR cloning and sequencing. This sequence was highly homologous to the corresponding fragment of the chicken AGRP gene. The western blotting analysis of adrenal gland and hypothalamus tissue extracts showed a well‐defined single band with an electrophoretic mobility consistent with the molecular weight of the avian AGRP protein. These results demonstrate that AGRP is expressed in the hypothalamus and adrenal glands of the duck and suggest an involvement of this peptide in the regulation of the melanocortin system in birds.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Expression of Agouti‐related Protein (Agrp) and its mRNA in the Hypothalamus and the Adrenal Gland of the Duck (Anas platyrhynchos)</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>Abstracts</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Expression of Agouti‐related Protein (Agrp) and its mRNA in the Hypothalamus and the Adrenal Gland of the Duck (Anas platyrhynchos)</title></titleInfo><name type="personal"><namePart type="given">N.</namePart><namePart type="family">Mirabella</namePart><affiliation>Department of structures, functions and biological technologies, Faculty of Veterinary Medicine, University of Naples Federico II, 80137, Naples, Italy</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">V.</namePart><namePart type="family">Esposito</namePart><affiliation>Department of structures, functions and biological technologies, Faculty of Veterinary Medicine, University of Naples Federico II, 80137, Naples, Italy</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">C.</namePart><namePart type="family">Squillacioti</namePart><affiliation>Department of structures, functions and biological technologies, Faculty of Veterinary Medicine, University of Naples Federico II, 80137, Naples, Italy</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">A.</namePart><namePart type="family">De Luca</namePart><affiliation>Department of structures, functions and biological technologies, Faculty of Veterinary Medicine, University of Naples Federico II, 80137, Naples, Italy</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">G.</namePart><namePart type="family">Germano</namePart><affiliation>Department of structures, functions and biological technologies, Faculty of Veterinary Medicine, University of Naples Federico II, 80137, Naples, Italy</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">G.</namePart><namePart type="family">Paino</namePart><affiliation>Department of structures, functions and biological technologies, Faculty of Veterinary Medicine, University of Naples Federico II, 80137, Naples, Italy</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="abstract" displayLabel="abstract"></genre><originInfo><publisher>Blackwell Verlag GmbH</publisher><place><placeTerm type="text">Berlin, Germany</placeTerm></place><dateIssued encoding="w3cdtf">2005-12</dateIssued><copyrightDate encoding="w3cdtf">2005</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract>Introduction: Agouti‐related protein (AGRP) is a neuropeptide involved in the control of body weight. Morphological and pharmacological studies have shown that AGRP is implicated in the central control of feeding behaviour acting as an endogenous antagonist of the alpha‐melanocyte stimulating hormone (α‐MSH), a potent satiety‐inducing factor, at the melanocortin 3 (MC3)‐ and four (MC4)‐receptors.</abstract><abstract>Aim: The aim of the present study was to investigate the expression of AGRP and its mRNA in the hypothalamus and adrenal gland of the duck and, in particular, to establish which type of adrenal tissue is involved in the AGRP synthesis.</abstract><abstract>Methods: Immunohistochemistry, western blotting, reverse transcriptase (RT)‐polimerase chain reaction (PCR).</abstract><abstract>Results and Discussion: AGRP‐immunoreactivity was observed in neurons and nerve fibres in a restricted area of the hypothalamus. AGRP‐ir neurons were located in the nucleus infundibularis and distributed ventromedially to the third ventricle in the hypothalamic tuberal region. These neurons were round or, with a lesser extent, elongated in shape. AGRP‐ir fibres were seen to project to the median eminence (ME) and anterior periventricular hypothalamus. The AGRP ir‐fibres in the ME were distributed in the external layer in close vicinity to the capillaries of the hypothalamo‐hypophysial portal system. In the avian adrenal gland, AGRP immunoreactivity was observed in the medullary tissue. A partial sequence of AGRP cDNA was identified using RT‐PCR cloning and sequencing. This sequence was highly homologous to the corresponding fragment of the chicken AGRP gene. The western blotting analysis of adrenal gland and hypothalamus tissue extracts showed a well‐defined single band with an electrophoretic mobility consistent with the molecular weight of the avian AGRP protein. These results demonstrate that AGRP is expressed in the hypothalamus and adrenal glands of the duck and suggest an involvement of this peptide in the regulation of the melanocortin system in birds.</abstract><relatedItem type="host"><titleInfo><title>Anatomia, Histologia, Embryologia</title></titleInfo><genre type="journal">journal</genre><identifier type="ISSN">0340-2096</identifier><identifier type="eISSN">1439-0264</identifier><identifier type="DOI">10.1111/(ISSN)1439-0264</identifier><identifier type="PublisherID">AHE</identifier><part><date>2005</date><detail type="volume"><caption>vol.</caption><number>34</number></detail><detail type="supplement"><caption>Suppl. no.</caption><number>s1</number></detail><extent unit="pages"><start>33</start><end>33</end></extent></part></relatedItem><identifier type="istex">42FFFC7EA512C2C04659B542CC5A894BED79C14C</identifier><identifier type="DOI">10.1111/j.1439-0264.2005.00669_75.x</identifier><identifier type="ArticleID">AHE669_75_75</identifier><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>Blackwell Verlag GmbH</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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