Isolation and cDNA Cloning of Somatolactin in Rabbitfish ( Siganus guttatus )
Identifieur interne : 000942 ( Istex/Corpus ); précédent : 000941; suivant : 000943Isolation and cDNA Cloning of Somatolactin in Rabbitfish ( Siganus guttatus )
Auteurs : Felix G. Ayson ; Evelyn Grace T. De Jesus ; Yutaka Amemiya ; Shunsuke Moriyama ; Tetsuya Hirano ; Hiroshi KawauchiSource :
- General and Comparative Endocrinology [ 0016-6480 ] ; 1999.
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Abstract
We report the isolation and cDNA cloning of somatolactin (SL) from rabbitfish, Siganus guttatus. Rabbitfish SL was isolated from an alkaline extract of the pituitary glands by gel filtration chromatography on Sephadex G-100 and reversed-phase high-performance liquid chromatography. SL was monitored by immunoblotting with flounder SL antiserum. The preparation (yield: 0.86 mg/g wet tissues) contained two immunoreactive bands of 24 and 28 kDa on SDS–PAGE. Overlapping partial cDNA clones corresponding to teleost SLs were amplified by PCR from single-strand cDNA from pituitary glands. Excluding the poly(A) tail, rabbitfish SL cDNA is 1605 bp long. It contains a 693-bp open reading frame encoding a signal peptide of 24 amino acids (aa) and a mature protein of 207 aa. Rabbitfish SL has two possible N-glycosylation sites at positions 11 and 121 and seven half Cys residues. The deduced amino acid sequence shows over 80% identity with those of advanced teleosts like sea bream, red drum, and flounder, 76% with the salmonids, 57% with the eel, and 46% with the goldfish SL.
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DOI: 10.1006/gcen.1999.7315
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ISTEX:2EDBA9B927A0BD795F771E84180FA447FC576C68Le document en format XML
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Rabbitfish SL was isolated from an alkaline extract of the pituitary glands by gel filtration chromatography on Sephadex G-100 and reversed-phase high-performance liquid chromatography. SL was monitored by immunoblotting with flounder SL antiserum. The preparation (yield: 0.86 mg/g wet tissues) contained two immunoreactive bands of 24 and 28 kDa on SDS–PAGE. Overlapping partial cDNA clones corresponding to teleost SLs were amplified by PCR from single-strand cDNA from pituitary glands. Excluding the poly(A) tail, rabbitfish SL cDNA is 1605 bp long. It contains a 693-bp open reading frame encoding a signal peptide of 24 amino acids (aa) and a mature protein of 207 aa. Rabbitfish SL has two possible N-glycosylation sites at positions 11 and 121 and seven half Cys residues. The deduced amino acid sequence shows over 80% identity with those of advanced teleosts like sea bream, red drum, and flounder, 76% with the salmonids, 57% with the eel, and 46% with the goldfish SL.</div></front></TEI><istex><corpusName>elsevier</corpusName><author><json:item><name>Felix G. 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xml:id="author-6"><persName><forename type="first">Hiroshi</forename><surname>Kawauchi</surname></persName><affiliation>Laboratory of Molecular Endocrinology, School of Fisheries Sciences, Kitasato University, Sanriku, Iwate, 022-0101, Japan</affiliation></author></analytic><monogr><title level="j">General and Comparative Endocrinology</title><title level="j" type="abbrev">YGCEN</title><idno type="pISSN">0016-6480</idno><idno type="PII">S0016-6480(00)X0031-7</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1999"></date><biblScope unit="volume">115</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="292">292</biblScope><biblScope unit="page" to="300">300</biblScope></imprint></monogr><idno type="istex">2EDBA9B927A0BD795F771E84180FA447FC576C68</idno><idno type="DOI">10.1006/gcen.1999.7315</idno><idno 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Rabbitfish SL was isolated from an alkaline extract of the pituitary glands by gel filtration chromatography on Sephadex G-100 and reversed-phase high-performance liquid chromatography. SL was monitored by immunoblotting with flounder SL antiserum. The preparation (yield: 0.86 mg/g wet tissues) contained two immunoreactive bands of 24 and 28 kDa on SDS–PAGE. Overlapping partial cDNA clones corresponding to teleost SLs were amplified by PCR from single-strand cDNA from pituitary glands. Excluding the poly(A) tail, rabbitfish SL cDNA is 1605 bp long. It contains a 693-bp open reading frame encoding a signal peptide of 24 amino acids (aa) and a mature protein of 207 aa. Rabbitfish SL has two possible N-glycosylation sites at positions 11 and 121 and seven half Cys residues. The deduced amino acid sequence shows over 80% identity with those of advanced teleosts like sea bream, red drum, and flounder, 76% with the salmonids, 57% with the eel, and 46% with the goldfish SL.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>Keywords</head><item><term>rabbitfish</term></item><item><term>somatolactin.</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1999">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/2EDBA9B927A0BD795F771E84180FA447FC576C68/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla" xml:lang="en"><item-info><jid>YGCEN</jid><aid>97315</aid><ce:pii>S0016-6480(99)97315-X</ce:pii><ce:doi>10.1006/gcen.1999.7315</ce:doi><ce:copyright type="full-transfer" year="1999">Academic Press</ce:copyright></item-info><head><ce:article-footnote><ce:label>☆</ce:label><ce:note-para>The nucleotide sequence data reported in this paper have been deposited in the DDBJ/EMBL/GenBank databases under Accession No. AB026186.</ce:note-para></ce:article-footnote><ce:dochead><ce:textfn>Regular Article</ce:textfn></ce:dochead><ce:title>Isolation and cDNA Cloning of Somatolactin in Rabbitfish (<ce:italic>Siganus guttatus</ce:italic>)</ce:title><ce:author-group><ce:author><ce:given-name>Felix G.</ce:given-name><ce:surname>Ayson</ce:surname><ce:cross-ref refid="A1"><ce:sup>a</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Evelyn Grace T.</ce:given-name><ce:surname>de Jesus</ce:surname><ce:cross-ref refid="A1"><ce:sup>a</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Yutaka</ce:given-name><ce:surname>Amemiya</ce:surname><ce:cross-ref refid="A2"><ce:sup>b</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Shunsuke</ce:given-name><ce:surname>Moriyama</ce:surname><ce:cross-ref refid="A2"><ce:sup>b</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Tetsuya</ce:given-name><ce:surname>Hirano</ce:surname><ce:cross-ref refid="A3"><ce:sup>c</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Hiroshi</ce:given-name><ce:surname>Kawauchi</ce:surname><ce:cross-ref refid="A2"><ce:sup>b</ce:sup></ce:cross-ref></ce:author><ce:affiliation id="A1"><ce:label>a</ce:label><ce:textfn>Aquaculture Department, Southeast Asian Fisheries Development Center (SEAFDEC AQD), Tigbauan, 5021, Iloilo, Philippines</ce:textfn></ce:affiliation><ce:affiliation id="A2"><ce:label>b</ce:label><ce:textfn>Laboratory of Molecular Endocrinology, School of Fisheries Sciences, Kitasato University, Sanriku, Iwate, 022-0101, Japan</ce:textfn></ce:affiliation><ce:affiliation id="A3"><ce:label>c</ce:label><ce:textfn>Hawaii Institute of Marine Biology, University of Hawaii, Kaneohe Bay, Hawaii, 96744</ce:textfn></ce:affiliation></ce:author-group><ce:date-accepted day="26" month="4" year="1999"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>We report the isolation and cDNA cloning of somatolactin (SL) from rabbitfish, <ce:italic>Siganus guttatus.</ce:italic> Rabbitfish SL was isolated from an alkaline extract of the pituitary glands by gel filtration chromatography on Sephadex G-100 and reversed-phase high-performance liquid chromatography. SL was monitored by immunoblotting with flounder SL antiserum. The preparation (yield: 0.86 mg/g wet tissues) contained two immunoreactive bands of 24 and 28 kDa on SDS–PAGE. Overlapping partial cDNA clones corresponding to teleost SLs were amplified by PCR from single-strand cDNA from pituitary glands. Excluding the poly(A) tail, rabbitfish SL cDNA is 1605 bp long. It contains a 693-bp open reading frame encoding a signal peptide of 24 amino acids (aa) and a mature protein of 207 aa. Rabbitfish SL has two possible N-glycosylation sites at positions 11 and 121 and seven half Cys residues. The deduced amino acid sequence shows over 80% identity with those of advanced teleosts like sea bream, red drum, and flounder, 76% with the salmonids, 57% with the eel, and 46% with the goldfish SL.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords><ce:section-title>Keywords</ce:section-title><ce:keyword><ce:text>rabbitfish</ce:text></ce:keyword><ce:keyword><ce:text>somatolactin.</ce:text></ce:keyword></ce:keywords></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Isolation and cDNA Cloning of Somatolactin in Rabbitfish ( Siganus guttatus )</title></titleInfo><titleInfo type="alternative" lang="en" contentType="CDATA"><title>Isolation and cDNA Cloning of Somatolactin in Rabbitfish (</title></titleInfo><name type="personal"><namePart type="given">Felix G.</namePart><namePart type="family">Ayson</namePart><affiliation>Aquaculture Department, Southeast Asian Fisheries Development Center (SEAFDEC AQD), Tigbauan, 5021, Iloilo, Philippines</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Evelyn Grace T.</namePart><namePart type="family">de Jesus</namePart><affiliation>Aquaculture Department, Southeast Asian Fisheries Development Center (SEAFDEC AQD), Tigbauan, 5021, Iloilo, Philippines</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Yutaka</namePart><namePart type="family">Amemiya</namePart><affiliation>Laboratory of Molecular Endocrinology, School of Fisheries Sciences, Kitasato University, Sanriku, Iwate, 022-0101, Japan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Shunsuke</namePart><namePart type="family">Moriyama</namePart><affiliation>Laboratory of Molecular Endocrinology, School of Fisheries Sciences, Kitasato University, Sanriku, Iwate, 022-0101, Japan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Tetsuya</namePart><namePart type="family">Hirano</namePart><affiliation>Hawaii Institute of Marine Biology, University of Hawaii, Kaneohe Bay, Hawaii, 96744</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Hiroshi</namePart><namePart type="family">Kawauchi</namePart><affiliation>Laboratory of Molecular Endocrinology, School of Fisheries Sciences, Kitasato University, Sanriku, Iwate, 022-0101, Japan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article"></genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1999</dateIssued><copyrightDate encoding="w3cdtf">1999</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">We report the isolation and cDNA cloning of somatolactin (SL) from rabbitfish, Siganus guttatus. Rabbitfish SL was isolated from an alkaline extract of the pituitary glands by gel filtration chromatography on Sephadex G-100 and reversed-phase high-performance liquid chromatography. SL was monitored by immunoblotting with flounder SL antiserum. The preparation (yield: 0.86 mg/g wet tissues) contained two immunoreactive bands of 24 and 28 kDa on SDS–PAGE. Overlapping partial cDNA clones corresponding to teleost SLs were amplified by PCR from single-strand cDNA from pituitary glands. Excluding the poly(A) tail, rabbitfish SL cDNA is 1605 bp long. It contains a 693-bp open reading frame encoding a signal peptide of 24 amino acids (aa) and a mature protein of 207 aa. Rabbitfish SL has two possible N-glycosylation sites at positions 11 and 121 and seven half Cys residues. The deduced amino acid sequence shows over 80% identity with those of advanced teleosts like sea bream, red drum, and flounder, 76% with the salmonids, 57% with the eel, and 46% with the goldfish SL.</abstract><note>The nucleotide sequence data reported in this paper have been deposited in the DDBJ/EMBL/GenBank databases under Accession No. AB026186.</note><note type="content">Section title: Regular Article</note><subject lang="en"><genre>Keywords</genre><topic>rabbitfish</topic><topic>somatolactin.</topic></subject><relatedItem type="host"><titleInfo><title>General and Comparative Endocrinology</title></titleInfo><titleInfo type="abbreviated"><title>YGCEN</title></titleInfo><genre type="journal">journal</genre><originInfo><dateIssued encoding="w3cdtf">199908</dateIssued></originInfo><identifier type="ISSN">0016-6480</identifier><identifier type="PII">S0016-6480(00)X0031-7</identifier><part><date>199908</date><detail type="volume"><number>115</number><caption>vol.</caption></detail><detail type="issue"><number>2</number><caption>no.</caption></detail><extent unit="issue pages"><start>169</start><end>308</end></extent><extent unit="pages"><start>292</start><end>300</end></extent></part></relatedItem><identifier type="istex">2EDBA9B927A0BD795F771E84180FA447FC576C68</identifier><identifier type="DOI">10.1006/gcen.1999.7315</identifier><identifier type="PII">S0016-6480(99)97315-X</identifier><accessCondition type="use and reproduction" contentType="copyright">©1999 Academic Press</accessCondition><recordInfo><recordContentSource>ELSEVIER</recordContentSource><recordOrigin>Academic Press, ©1999</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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