Contrasting effects of estrogen on transthyretin and vitellogenin expression in males of the marine fish, Sparus aurata
Identifieur interne : 000929 ( Istex/Corpus ); précédent : 000928; suivant : 000930Contrasting effects of estrogen on transthyretin and vitellogenin expression in males of the marine fish, Sparus aurata
Auteurs : B. Funkenstein ; C. J Bowman ; N. D Denslow ; M. Cardinali ; O. CarnevaliSource :
- Molecular and Cellular Endocrinology [ 0303-7207 ] ; 2000.
English descriptors
Abstract
A partial cDNA encoding for the C-terminus of vitellogenin (VTG) was cloned from liver of Sparus aurata male treated with 17β-estradiol (E2). E2 treatment of S. aurata males resulted in increased synthesis and secretion of VTG protein into the plasma, determined by a specific enzyme-linked immunosorbent assay (ELISA) in a time-dependent manner. While VTG mRNA was induced by E2 treatment, transthyretin (TTR) mRNA levels were reduced. These data provide the first demonstration that estrogen exhibits contrasting effect on VTG and on TTR gene expression in teleosts.
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DOI: 10.1016/S0303-7207(00)00301-4
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ISTEX:8AE9D710438FB1E491F287ABCDA8250E8B155A56Le document en format XML
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J Bowman</name><affiliation><mods:affiliation>Department of Biochemistry and Molecular Biology, University of Florida, 1600 S.W. Archer Road, Box 100245, Gainesville, FL 32610, USA</mods:affiliation></affiliation></author><author><name sortKey="Denslow, N D" sort="Denslow, N D" uniqKey="Denslow N" first="N. D" last="Denslow">N. D Denslow</name><affiliation><mods:affiliation>Department of Biochemistry and Molecular Biology, University of Florida, 1600 S.W. Archer Road, Box 100245, Gainesville, FL 32610, USA</mods:affiliation></affiliation></author><author><name sortKey="Cardinali, M" sort="Cardinali, M" uniqKey="Cardinali M" first="M" last="Cardinali">M. Cardinali</name><affiliation><mods:affiliation>Department of Biology (MCA), University of Ancona, Via Brecce Bianche, Ancona, Italy</mods:affiliation></affiliation></author><author><name sortKey="Carnevali, O" sort="Carnevali, O" uniqKey="Carnevali O" first="O" last="Carnevali">O. Carnevali</name><affiliation><mods:affiliation>Department of Biology (MCA), University of Ancona, Via Brecce Bianche, Ancona, Italy</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:8AE9D710438FB1E491F287ABCDA8250E8B155A56</idno><date when="2000" year="2000">2000</date><idno type="doi">10.1016/S0303-7207(00)00301-4</idno><idno type="url">https://api.istex.fr/document/8AE9D710438FB1E491F287ABCDA8250E8B155A56/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000929</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000929</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Contrasting effects of estrogen on transthyretin and vitellogenin expression in males of the marine fish, Sparus aurata</title><author><name sortKey="Funkenstein, B" sort="Funkenstein, B" uniqKey="Funkenstein B" first="B" last="Funkenstein">B. Funkenstein</name><affiliation><mods:affiliation>National Institute of Oceanography, Israel Oceanographic and Limnological Research, Tel Shikmona, PO Box 8030, Haifa 31080, Israel</mods:affiliation></affiliation><affiliation><mods:affiliation>Corresponding author. Tel.: +972-4-8515202; fax: +972-4-8511911</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: bruria@ocean.org.il</mods:affiliation></affiliation></author><author><name sortKey="Bowman, C J" sort="Bowman, C J" uniqKey="Bowman C" first="C. J" last="Bowman">C. J Bowman</name><affiliation><mods:affiliation>Department of Biochemistry and Molecular Biology, University of Florida, 1600 S.W. Archer Road, Box 100245, Gainesville, FL 32610, USA</mods:affiliation></affiliation></author><author><name sortKey="Denslow, N D" sort="Denslow, N D" uniqKey="Denslow N" first="N. D" last="Denslow">N. D Denslow</name><affiliation><mods:affiliation>Department of Biochemistry and Molecular Biology, University of Florida, 1600 S.W. Archer Road, Box 100245, Gainesville, FL 32610, USA</mods:affiliation></affiliation></author><author><name sortKey="Cardinali, M" sort="Cardinali, M" uniqKey="Cardinali M" first="M" last="Cardinali">M. Cardinali</name><affiliation><mods:affiliation>Department of Biology (MCA), University of Ancona, Via Brecce Bianche, Ancona, Italy</mods:affiliation></affiliation></author><author><name sortKey="Carnevali, O" sort="Carnevali, O" uniqKey="Carnevali O" first="O" last="Carnevali">O. Carnevali</name><affiliation><mods:affiliation>Department of Biology (MCA), University of Ancona, Via Brecce Bianche, Ancona, Italy</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Molecular and Cellular Endocrinology</title><title level="j" type="abbrev">MCE</title><idno type="ISSN">0303-7207</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="2000">2000</date><biblScope unit="volume">167</biblScope><biblScope unit="issue">1–2</biblScope><biblScope unit="page" from="33">33</biblScope><biblScope unit="page" to="41">41</biblScope></imprint><idno type="ISSN">0303-7207</idno></series><idno type="istex">8AE9D710438FB1E491F287ABCDA8250E8B155A56</idno><idno type="DOI">10.1016/S0303-7207(00)00301-4</idno><idno type="PII">S0303-7207(00)00301-4</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0303-7207</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>ELISA</term><term>Fish</term><term>Liver</term><term>RNA blot hybridization</term><term>Transthyretin</term><term>Vitellogenin cDNA and protein</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A partial cDNA encoding for the C-terminus of vitellogenin (VTG) was cloned from liver of Sparus aurata male treated with 17β-estradiol (E2). E2 treatment of S. aurata males resulted in increased synthesis and secretion of VTG protein into the plasma, determined by a specific enzyme-linked immunosorbent assay (ELISA) in a time-dependent manner. While VTG mRNA was induced by E2 treatment, transthyretin (TTR) mRNA levels were reduced. These data provide the first demonstration that estrogen exhibits contrasting effect on VTG and on TTR gene expression in teleosts.</div></front></TEI><istex><corpusName>elsevier</corpusName><author><json:item><name>B Funkenstein</name><affiliations><json:string>National Institute of Oceanography, Israel Oceanographic and Limnological Research, Tel Shikmona, PO Box 8030, Haifa 31080, Israel</json:string><json:string>Corresponding author. Tel.: +972-4-8515202; fax: +972-4-8511911</json:string><json:string>E-mail: bruria@ocean.org.il</json:string></affiliations></json:item><json:item><name>C.J Bowman</name><affiliations><json:string>Department of Biochemistry and Molecular Biology, University of Florida, 1600 S.W. Archer Road, Box 100245, Gainesville, FL 32610, USA</json:string></affiliations></json:item><json:item><name>N.D Denslow</name><affiliations><json:string>Department of Biochemistry and Molecular Biology, University of Florida, 1600 S.W. Archer Road, Box 100245, Gainesville, FL 32610, USA</json:string></affiliations></json:item><json:item><name>M Cardinali</name><affiliations><json:string>Department of Biology (MCA), University of Ancona, Via Brecce Bianche, Ancona, Italy</json:string></affiliations></json:item><json:item><name>O Carnevali</name><affiliations><json:string>Department of Biology (MCA), University of Ancona, Via Brecce Bianche, Ancona, Italy</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Fish</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Liver</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Vitellogenin cDNA and protein</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Transthyretin</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>ELISA</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>RNA blot hybridization</value></json:item></subject><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>A partial cDNA encoding for the C-terminus of vitellogenin (VTG) was cloned from liver of Sparus aurata male treated with 17β-estradiol (E2). E2 treatment of S. aurata males resulted in increased synthesis and secretion of VTG protein into the plasma, determined by a specific enzyme-linked immunosorbent assay (ELISA) in a time-dependent manner. While VTG mRNA was induced by E2 treatment, transthyretin (TTR) mRNA levels were reduced. These data provide the first demonstration that estrogen exhibits contrasting effect on VTG and on TTR gene expression in teleosts.</abstract><qualityIndicators><score>6.032</score><pdfVersion>1.2</pdfVersion><pdfPageSize>589 x 792 pts</pdfPageSize><refBibsNative>true</refBibsNative><keywordCount>6</keywordCount><abstractCharCount>568</abstractCharCount><pdfWordCount>5401</pdfWordCount><pdfCharCount>32464</pdfCharCount><pdfPageCount>9</pdfPageCount><abstractWordCount>86</abstractWordCount></qualityIndicators><title>Contrasting effects of estrogen on transthyretin and vitellogenin expression in males of the marine fish, Sparus aurata</title><pii><json:string>S0303-7207(00)00301-4</json:string></pii><genre><json:string>research-article</json:string></genre><serie><volume>2</volume><editor><json:item><name>M.B. Sporn</name></json:item><json:item><name>A.B. Roberts</name></json:item><json:item><name>D.S. Goodman</name></json:item></editor><pages><last>88</last><first>42</first></pages><language><json:string>unknown</json:string></language><title>Retinoids</title></serie><host><volume>167</volume><pii><json:string>S0303-7207(00)X0083-4</json:string></pii><pages><last>41</last><first>33</first></pages><issn><json:string>0303-7207</json:string></issn><issue>1–2</issue><genre><json:string>journal</json:string></genre><language><json:string>unknown</json:string></language><title>Molecular and Cellular Endocrinology</title><publicationDate>2000</publicationDate></host><publicationDate>2000</publicationDate><copyrightDate>2000</copyrightDate><doi><json:string>10.1016/S0303-7207(00)00301-4</json:string></doi><id>8AE9D710438FB1E491F287ABCDA8250E8B155A56</id><score>0.024088638</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/document/8AE9D710438FB1E491F287ABCDA8250E8B155A56/fulltext/pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/document/8AE9D710438FB1E491F287ABCDA8250E8B155A56/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/8AE9D710438FB1E491F287ABCDA8250E8B155A56/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Contrasting effects of estrogen on transthyretin and vitellogenin expression in males of the marine fish, Sparus aurata</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>ELSEVIER</publisher><availability><p>©2000 Elsevier Science Ireland Ltd</p></availability><date>2000</date></publicationStmt><notesStmt><note type="content">Fig. 1: Characterization of S. aurata VTG cDNA. (A) RT-PCR of liver total RNA extracted from a male sacrificed 5 days after E2 injection. MW, molecular marker (100-bp ladder); VTG, vitellogenin PCR fragment. (B) Alignment of Sparus VTG with Fundulus VTG II (Accession No. Q98893), rainbow trout VTG (Accession No. Q92093) and Fundulus VTG I (Accession No. Q90508). Numbers above represent amino acid number of Fundulus VTG II. Dashes represent identical amino acids. (C) Northern blot analysis of VTG mRNA. C, control; and E, E2-treated males.</note><note type="content">Fig. 2: Effect of E2 treatment on VTG protein in plasma of male S. aurata. (A) SDS-gel electrophoresis (7% acrylamide) of male seabream plasma stained with Coomassie Blue. Plasma after 3 days (lane 1), 10 days (lane 2) and 30 days (lane 3) of E2 treatment, (lane 4) male plasma, (lane 5) purified seabream VTG. (B) Western blot analysis of male plasma after 3 days (lanes 1 and 2), 10 days (lanes 3 and 4) and 30 days (lanes 5 and 6) of E2 treatment, using seabream anti-vitellogenin antiserum. (7) male plasma and (8) purified seabream VTG. (C) Plasma VTG levels determined by ELISA in seabream males after 3, 10 and 30 days of E2 treatment. Results are expressed as mean±S.E. (n=8).</note><note type="content">Fig. 3: Effect of E2 treatment on TTR and VTG expression in seabream. RNA was fractionated on 1% agarose gels and transferred to nylon membranes. The blots were sequentially hybridized with Sparus TTR, Sparus VTG and Sparus β-actin cDNAs. (A) Total RNA (10 μg) prepared from liver of a mature male (M) or a female (F) during spawning season. (B) Poly(A+)RNA prepared from a liver of an adult male, untreated (C), and 5 days after E2 injection (E). (C) Total RNA (30 μg) prepared from a liver of an adult male, untreated (C), and 30 days after E2 treatment (E).</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Contrasting effects of estrogen on transthyretin and vitellogenin expression in males of the marine fish, Sparus aurata</title><author xml:id="author-1"><persName><forename type="first">B</forename><surname>Funkenstein</surname></persName><email>bruria@ocean.org.il</email><affiliation>National Institute of Oceanography, Israel Oceanographic and Limnological Research, Tel Shikmona, PO Box 8030, Haifa 31080, Israel</affiliation><affiliation>Corresponding author. Tel.: +972-4-8515202; fax: +972-4-8511911</affiliation></author><author xml:id="author-2"><persName><forename type="first">C.J</forename><surname>Bowman</surname></persName><affiliation>Department of Biochemistry and Molecular Biology, University of Florida, 1600 S.W. Archer Road, Box 100245, Gainesville, FL 32610, USA</affiliation></author><author xml:id="author-3"><persName><forename type="first">N.D</forename><surname>Denslow</surname></persName><affiliation>Department of Biochemistry and Molecular Biology, University of Florida, 1600 S.W. Archer Road, Box 100245, Gainesville, FL 32610, USA</affiliation></author><author xml:id="author-4"><persName><forename type="first">M</forename><surname>Cardinali</surname></persName><affiliation>Department of Biology (MCA), University of Ancona, Via Brecce Bianche, Ancona, Italy</affiliation></author><author xml:id="author-5"><persName><forename type="first">O</forename><surname>Carnevali</surname></persName><affiliation>Department of Biology (MCA), University of Ancona, Via Brecce Bianche, Ancona, Italy</affiliation></author></analytic><monogr><title level="j">Molecular and Cellular Endocrinology</title><title level="j" type="abbrev">MCE</title><idno type="pISSN">0303-7207</idno><idno type="PII">S0303-7207(00)X0083-4</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="2000"></date><biblScope unit="volume">167</biblScope><biblScope unit="issue">1–2</biblScope><biblScope unit="page" from="33">33</biblScope><biblScope unit="page" to="41">41</biblScope></imprint></monogr><idno type="istex">8AE9D710438FB1E491F287ABCDA8250E8B155A56</idno><idno type="DOI">10.1016/S0303-7207(00)00301-4</idno><idno type="PII">S0303-7207(00)00301-4</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2000</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>A partial cDNA encoding for the C-terminus of vitellogenin (VTG) was cloned from liver of Sparus aurata male treated with 17β-estradiol (E2). E2 treatment of S. aurata males resulted in increased synthesis and secretion of VTG protein into the plasma, determined by a specific enzyme-linked immunosorbent assay (ELISA) in a time-dependent manner. While VTG mRNA was induced by E2 treatment, transthyretin (TTR) mRNA levels were reduced. These data provide the first demonstration that estrogen exhibits contrasting effect on VTG and on TTR gene expression in teleosts.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>Keywords</head><item><term>Fish</term></item><item><term>Liver</term></item><item><term>Vitellogenin cDNA and protein</term></item><item><term>Transthyretin</term></item><item><term>ELISA</term></item><item><term>RNA blot hybridization</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2000">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/8AE9D710438FB1E491F287ABCDA8250E8B155A56/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: ce:floats; body; tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"><istex:entity SYSTEM="gr1a" NDATA="IMAGE" name="gr1a"></istex:entity><istex:entity SYSTEM="gr1b" NDATA="IMAGE" name="gr1b"></istex:entity><istex:entity SYSTEM="gr1c" NDATA="IMAGE" name="gr1c"></istex:entity><istex:entity SYSTEM="gr2" NDATA="IMAGE" name="gr2"></istex:entity><istex:entity SYSTEM="gr3" NDATA="IMAGE" name="gr3"></istex:entity></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla" xml:lang="en"><item-info><jid>MCE</jid><aid>4871</aid><ce:pii>S0303-7207(00)00301-4</ce:pii><ce:doi>10.1016/S0303-7207(00)00301-4</ce:doi><ce:copyright type="full-transfer" year="2000">Elsevier Science Ireland Ltd</ce:copyright></item-info><head><ce:title>Contrasting effects of estrogen on transthyretin and vitellogenin expression in males of the marine fish, <ce:italic>Sparus aurata</ce:italic></ce:title><ce:author-group><ce:author><ce:given-name>B</ce:given-name><ce:surname>Funkenstein</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>a</ce:sup></ce:cross-ref><ce:cross-ref refid="CORR1">*</ce:cross-ref><ce:e-address>bruria@ocean.org.il</ce:e-address></ce:author><ce:author><ce:given-name>C.J</ce:given-name><ce:surname>Bowman</ce:surname><ce:cross-ref refid="AFF2"><ce:sup>b</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>N.D</ce:given-name><ce:surname>Denslow</ce:surname><ce:cross-ref refid="AFF2"><ce:sup>b</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>M</ce:given-name><ce:surname>Cardinali</ce:surname><ce:cross-ref refid="AFF3"><ce:sup>c</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>O</ce:given-name><ce:surname>Carnevali</ce:surname><ce:cross-ref refid="AFF3"><ce:sup>c</ce:sup></ce:cross-ref></ce:author><ce:affiliation id="AFF1"><ce:label>a</ce:label><ce:textfn>National Institute of Oceanography, Israel Oceanographic and Limnological Research, Tel Shikmona, PO Box 8030, Haifa 31080, Israel</ce:textfn></ce:affiliation><ce:affiliation id="AFF2"><ce:label>b</ce:label><ce:textfn>Department of Biochemistry and Molecular Biology, University of Florida, 1600 S.W. Archer Road, Box 100245, Gainesville, FL 32610, USA</ce:textfn></ce:affiliation><ce:affiliation id="AFF3"><ce:label>c</ce:label><ce:textfn>Department of Biology (MCA), University of Ancona, Via Brecce Bianche, Ancona, Italy</ce:textfn></ce:affiliation><ce:correspondence id="CORR1"><ce:label>*</ce:label><ce:text>Corresponding author. Tel.: +972-4-8515202; fax: +972-4-8511911</ce:text></ce:correspondence></ce:author-group><ce:date-received day="22" month="2" year="2000"></ce:date-received><ce:date-accepted day="12" month="6" year="2000"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>A partial cDNA encoding for the C-terminus of vitellogenin (VTG) was cloned from liver of <ce:italic>Sparus aurata</ce:italic> male treated with 17β-estradiol (E<ce:inf>2</ce:inf>). E<ce:inf>2</ce:inf> treatment of <ce:italic>S. aurata</ce:italic> males resulted in increased synthesis and secretion of VTG protein into the plasma, determined by a specific enzyme-linked immunosorbent assay (ELISA) in a time-dependent manner. While VTG mRNA was induced by E<ce:inf>2</ce:inf> treatment, transthyretin (TTR) mRNA levels were reduced. These data provide the first demonstration that estrogen exhibits contrasting effect on VTG and on TTR gene expression in teleosts.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords class="keyword"><ce:section-title>Keywords</ce:section-title><ce:keyword><ce:text>Fish</ce:text></ce:keyword><ce:keyword><ce:text>Liver</ce:text></ce:keyword><ce:keyword><ce:text>Vitellogenin cDNA and protein</ce:text></ce:keyword><ce:keyword><ce:text>Transthyretin</ce:text></ce:keyword><ce:keyword><ce:text>ELISA</ce:text></ce:keyword><ce:keyword><ce:text>RNA blot hybridization</ce:text></ce:keyword></ce:keywords></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Contrasting effects of estrogen on transthyretin and vitellogenin expression in males of the marine fish, Sparus aurata</title></titleInfo><titleInfo type="alternative" lang="en" contentType="CDATA"><title>Contrasting effects of estrogen on transthyretin and vitellogenin expression in males of the marine fish,</title></titleInfo><name type="personal"><namePart type="given">B</namePart><namePart type="family">Funkenstein</namePart><affiliation>National Institute of Oceanography, Israel Oceanographic and Limnological Research, Tel Shikmona, PO Box 8030, Haifa 31080, Israel</affiliation><affiliation>Corresponding author. Tel.: +972-4-8515202; fax: +972-4-8511911</affiliation><affiliation>E-mail: bruria@ocean.org.il</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">C.J</namePart><namePart type="family">Bowman</namePart><affiliation>Department of Biochemistry and Molecular Biology, University of Florida, 1600 S.W. Archer Road, Box 100245, Gainesville, FL 32610, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">N.D</namePart><namePart type="family">Denslow</namePart><affiliation>Department of Biochemistry and Molecular Biology, University of Florida, 1600 S.W. Archer Road, Box 100245, Gainesville, FL 32610, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M</namePart><namePart type="family">Cardinali</namePart><affiliation>Department of Biology (MCA), University of Ancona, Via Brecce Bianche, Ancona, Italy</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">O</namePart><namePart type="family">Carnevali</namePart><affiliation>Department of Biology (MCA), University of Ancona, Via Brecce Bianche, Ancona, Italy</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article"></genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">2000</dateIssued><copyrightDate encoding="w3cdtf">2000</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">A partial cDNA encoding for the C-terminus of vitellogenin (VTG) was cloned from liver of Sparus aurata male treated with 17β-estradiol (E2). E2 treatment of S. aurata males resulted in increased synthesis and secretion of VTG protein into the plasma, determined by a specific enzyme-linked immunosorbent assay (ELISA) in a time-dependent manner. While VTG mRNA was induced by E2 treatment, transthyretin (TTR) mRNA levels were reduced. These data provide the first demonstration that estrogen exhibits contrasting effect on VTG and on TTR gene expression in teleosts.</abstract><note type="content">Fig. 1: Characterization of S. aurata VTG cDNA. (A) RT-PCR of liver total RNA extracted from a male sacrificed 5 days after E2 injection. MW, molecular marker (100-bp ladder); VTG, vitellogenin PCR fragment. (B) Alignment of Sparus VTG with Fundulus VTG II (Accession No. Q98893), rainbow trout VTG (Accession No. Q92093) and Fundulus VTG I (Accession No. Q90508). Numbers above represent amino acid number of Fundulus VTG II. Dashes represent identical amino acids. (C) Northern blot analysis of VTG mRNA. C, control; and E, E2-treated males.</note><note type="content">Fig. 2: Effect of E2 treatment on VTG protein in plasma of male S. aurata. (A) SDS-gel electrophoresis (7% acrylamide) of male seabream plasma stained with Coomassie Blue. Plasma after 3 days (lane 1), 10 days (lane 2) and 30 days (lane 3) of E2 treatment, (lane 4) male plasma, (lane 5) purified seabream VTG. (B) Western blot analysis of male plasma after 3 days (lanes 1 and 2), 10 days (lanes 3 and 4) and 30 days (lanes 5 and 6) of E2 treatment, using seabream anti-vitellogenin antiserum. (7) male plasma and (8) purified seabream VTG. (C) Plasma VTG levels determined by ELISA in seabream males after 3, 10 and 30 days of E2 treatment. Results are expressed as mean±S.E. (n=8).</note><note type="content">Fig. 3: Effect of E2 treatment on TTR and VTG expression in seabream. RNA was fractionated on 1% agarose gels and transferred to nylon membranes. The blots were sequentially hybridized with Sparus TTR, Sparus VTG and Sparus β-actin cDNAs. (A) Total RNA (10 μg) prepared from liver of a mature male (M) or a female (F) during spawning season. (B) Poly(A+)RNA prepared from a liver of an adult male, untreated (C), and 5 days after E2 injection (E). (C) Total RNA (30 μg) prepared from a liver of an adult male, untreated (C), and 30 days after E2 treatment (E).</note><subject lang="en"><genre>Keywords</genre><topic>Fish</topic><topic>Liver</topic><topic>Vitellogenin cDNA and protein</topic><topic>Transthyretin</topic><topic>ELISA</topic><topic>RNA blot hybridization</topic></subject><relatedItem type="host"><titleInfo><title>Molecular and Cellular Endocrinology</title></titleInfo><titleInfo type="abbreviated"><title>MCE</title></titleInfo><genre type="journal">journal</genre><originInfo><dateIssued encoding="w3cdtf">20000925</dateIssued></originInfo><identifier type="ISSN">0303-7207</identifier><identifier type="PII">S0303-7207(00)X0083-4</identifier><part><date>20000925</date><detail type="volume"><number>167</number><caption>vol.</caption></detail><detail type="issue"><number>1–2</number><caption>no.</caption></detail><extent unit="issue pages"><start>1</start><end>162</end></extent><extent unit="pages"><start>33</start><end>41</end></extent></part></relatedItem><identifier type="istex">8AE9D710438FB1E491F287ABCDA8250E8B155A56</identifier><identifier type="DOI">10.1016/S0303-7207(00)00301-4</identifier><identifier type="PII">S0303-7207(00)00301-4</identifier><accessCondition type="use and reproduction" contentType="copyright">©2000 Elsevier Science Ireland Ltd</accessCondition><recordInfo><recordContentSource>ELSEVIER</recordContentSource><recordOrigin>Elsevier Science Ireland Ltd, ©2000</recordOrigin></recordInfo></mods></metadata></istex></record>Pour manipuler ce document sous Unix (Dilib)
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