Pituitary gonadotropic hormone from a chondrostean fish, starred sturgeon ( Acipenser stellatus Pall.)
Identifieur interne : 000915 ( Istex/Corpus ); précédent : 000914; suivant : 000916Pituitary gonadotropic hormone from a chondrostean fish, starred sturgeon ( Acipenser stellatus Pall.)
Auteurs : A. A. Kuznetzov ; B. F. Goncharov ; E. Burzawa-GerardSource :
- General and Comparative Endocrinology [ 0016-6480 ] ; 1983.
Abstract
Four biologically active fractions of gonadotropic hormone (aci-GTH-A, -B, -C, -D) were isolated and purified from acetonized pituitaries of the starred sturgeon (Acipenser stellatus Pall.). Their separation was achieved by DEAE-cellulose chromatography. Disc-electrophoresis and especially isoelectric focusing in polyacrylamide gel showed that each fraction contained several components. Not less than 15 different components as a whole with isoelectric points ranging from 4.5 to 7.0 could be counted in four aci-GTH preparations. All these components were active in toad oocyte maturation test. Only two of four preparations (aci-GTH-A and -D) were practically free of common components. All aci-GTH preparations were shown to be homogeneous and identical by molecular weight, sedimentation coefficient, sialic acid content, and some immunological properties. N-terminal amino acid analysis revealed tyrosine and leucine in all aci-GTH preparations, with the only exception of aci-GTH-D that contained an additional polypeptide with N-terminal glycine. No differences in the spectra of aci-GTH isoforms were found when pituitary extract, newly purified or 3 years older hormone preparations were submitted to isoelectric focusing.
Url:
DOI: 10.1016/0016-6480(83)90201-0
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Burzawa-Gerard</name><affiliation><mods:affiliation>Laboratoire de Physiologie Générale et comparée et Laboratoire d'Endocrinologie comparée associé au CNRS, Muséum national d'Histoire naturelle, 7 rue Cuvier, 75230 Paris Cedex 5, France</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:2B85ACF483F193588431A7D60C0CDDCFCDECCE5A</idno><date when="1983" year="1983">1983</date><idno type="doi">10.1016/0016-6480(83)90201-0</idno><idno type="url">https://api.istex.fr/document/2B85ACF483F193588431A7D60C0CDDCFCDECCE5A/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000915</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000915</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Pituitary gonadotropic hormone from a chondrostean fish, starred sturgeon ( Acipenser stellatus Pall.)</title><author><name sortKey="Kuznetzov, A A" sort="Kuznetzov, A A" uniqKey="Kuznetzov A" first="A. A." last="Kuznetzov">A. A. Kuznetzov</name><affiliation><mods:affiliation>N. K. Koltzov Institute of Developmental Biology, USSR Academy of Sciences, 26 Vavilov St., Moscow 177 808, USSR</mods:affiliation></affiliation></author><author><name sortKey="Goncharov, B F" sort="Goncharov, B F" uniqKey="Goncharov B" first="B. F." last="Goncharov">B. F. Goncharov</name><affiliation><mods:affiliation>N. K. Koltzov Institute of Developmental Biology, USSR Academy of Sciences, 26 Vavilov St., Moscow 177 808, USSR</mods:affiliation></affiliation></author><author><name sortKey="Burzawa Gerard, E" sort="Burzawa Gerard, E" uniqKey="Burzawa Gerard E" first="E." last="Burzawa-Gerard">E. Burzawa-Gerard</name><affiliation><mods:affiliation>Laboratoire de Physiologie Générale et comparée et Laboratoire d'Endocrinologie comparée associé au CNRS, Muséum national d'Histoire naturelle, 7 rue Cuvier, 75230 Paris Cedex 5, France</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">General and Comparative Endocrinology</title><title level="j" type="abbrev">YGCEN</title><idno type="ISSN">0016-6480</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1983">1983</date><biblScope unit="volume">49</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="364">364</biblScope><biblScope unit="page" to="374">374</biblScope></imprint><idno type="ISSN">0016-6480</idno></series><idno type="istex">2B85ACF483F193588431A7D60C0CDDCFCDECCE5A</idno><idno type="DOI">10.1016/0016-6480(83)90201-0</idno><idno type="PII">0016-6480(83)90201-0</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0016-6480</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Four biologically active fractions of gonadotropic hormone (aci-GTH-A, -B, -C, -D) were isolated and purified from acetonized pituitaries of the starred sturgeon (Acipenser stellatus Pall.). Their separation was achieved by DEAE-cellulose chromatography. Disc-electrophoresis and especially isoelectric focusing in polyacrylamide gel showed that each fraction contained several components. Not less than 15 different components as a whole with isoelectric points ranging from 4.5 to 7.0 could be counted in four aci-GTH preparations. All these components were active in toad oocyte maturation test. Only two of four preparations (aci-GTH-A and -D) were practically free of common components. All aci-GTH preparations were shown to be homogeneous and identical by molecular weight, sedimentation coefficient, sialic acid content, and some immunological properties. N-terminal amino acid analysis revealed tyrosine and leucine in all aci-GTH preparations, with the only exception of aci-GTH-D that contained an additional polypeptide with N-terminal glycine. No differences in the spectra of aci-GTH isoforms were found when pituitary extract, newly purified or 3 years older hormone preparations were submitted to isoelectric focusing.</div></front></TEI><istex><corpusName>elsevier</corpusName><author><json:item><name>A.A. Kuznetzov</name><affiliations><json:string>N. K. Koltzov Institute of Developmental Biology, USSR Academy of Sciences, 26 Vavilov St., Moscow 177 808, USSR</json:string></affiliations></json:item><json:item><name>B.F. Goncharov</name><affiliations><json:string>N. K. Koltzov Institute of Developmental Biology, USSR Academy of Sciences, 26 Vavilov St., Moscow 177 808, USSR</json:string></affiliations></json:item><json:item><name>E. 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All these components were active in toad oocyte maturation test. Only two of four preparations (aci-GTH-A and -D) were practically free of common components. All aci-GTH preparations were shown to be homogeneous and identical by molecular weight, sedimentation coefficient, sialic acid content, and some immunological properties. N-terminal amino acid analysis revealed tyrosine and leucine in all aci-GTH preparations, with the only exception of aci-GTH-D that contained an additional polypeptide with N-terminal glycine. 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Koltzov Institute of Developmental Biology, USSR Academy of Sciences, 26 Vavilov St., Moscow 177 808, USSR</affiliation></author><author xml:id="author-3"><persName><forename type="first">E.</forename><surname>Burzawa-Gerard</surname></persName><affiliation>Laboratoire de Physiologie Générale et comparée et Laboratoire d'Endocrinologie comparée associé au CNRS, Muséum national d'Histoire naturelle, 7 rue Cuvier, 75230 Paris Cedex 5, France</affiliation></author></analytic><monogr><title level="j">General and Comparative Endocrinology</title><title level="j" type="abbrev">YGCEN</title><idno type="pISSN">0016-6480</idno><idno type="PII">S0016-6480(00)X0248-1</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1983"></date><biblScope unit="volume">49</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="364">364</biblScope><biblScope unit="page" to="374">374</biblScope></imprint></monogr><idno type="istex">2B85ACF483F193588431A7D60C0CDDCFCDECCE5A</idno><idno type="DOI">10.1016/0016-6480(83)90201-0</idno><idno type="PII">0016-6480(83)90201-0</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1983</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Four biologically active fractions of gonadotropic hormone (aci-GTH-A, -B, -C, -D) were isolated and purified from acetonized pituitaries of the starred sturgeon (Acipenser stellatus Pall.). Their separation was achieved by DEAE-cellulose chromatography. Disc-electrophoresis and especially isoelectric focusing in polyacrylamide gel showed that each fraction contained several components. Not less than 15 different components as a whole with isoelectric points ranging from 4.5 to 7.0 could be counted in four aci-GTH preparations. All these components were active in toad oocyte maturation test. Only two of four preparations (aci-GTH-A and -D) were practically free of common components. All aci-GTH preparations were shown to be homogeneous and identical by molecular weight, sedimentation coefficient, sialic acid content, and some immunological properties. N-terminal amino acid analysis revealed tyrosine and leucine in all aci-GTH preparations, with the only exception of aci-GTH-D that contained an additional polypeptide with N-terminal glycine. No differences in the spectra of aci-GTH isoforms were found when pituitary extract, newly purified or 3 years older hormone preparations were submitted to isoelectric focusing.</p></abstract></profileDesc><revisionDesc><change when="1983">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/2B85ACF483F193588431A7D60C0CDDCFCDECCE5A/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>YGCEN</jid><aid>83902010</aid><ce:pii>0016-6480(83)90201-0</ce:pii><ce:doi>10.1016/0016-6480(83)90201-0</ce:doi><ce:copyright type="unknown" year="1983"></ce:copyright></item-info><head><ce:title>Pituitary gonadotropic hormone from a chondrostean fish, starred sturgeon (<ce:italic>Acipenser stellatus</ce:italic> Pall.)</ce:title><ce:subtitle>III. Polymorphism</ce:subtitle><ce:author-group><ce:author><ce:given-name>A.A.</ce:given-name><ce:surname>Kuznetzov</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>a</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>B.F.</ce:given-name><ce:surname>Goncharov</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>a</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>E.</ce:given-name><ce:surname>Burzawa-Gerard</ce:surname><ce:cross-ref refid="AFF2"><ce:sup>∗</ce:sup></ce:cross-ref></ce:author><ce:affiliation id="AFF1"><ce:label>a</ce:label><ce:textfn>N. K. Koltzov Institute of Developmental Biology, USSR Academy of Sciences, 26 Vavilov St., Moscow 177 808, USSR</ce:textfn></ce:affiliation><ce:affiliation id="AFF2"><ce:label>b</ce:label><ce:textfn>Laboratoire de Physiologie Générale et comparée et Laboratoire d'Endocrinologie comparée associé au CNRS, Muséum national d'Histoire naturelle, 7 rue Cuvier, 75230 Paris Cedex 5, France</ce:textfn></ce:affiliation></ce:author-group><ce:date-accepted day="30" month="3" year="1982"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>Four biologically active fractions of gonadotropic hormone (aci-GTH-A, -B, -C, -D) were isolated and purified from acetonized pituitaries of the starred sturgeon (<ce:italic>Acipenser stellatus</ce:italic> Pall.). Their separation was achieved by DEAE-cellulose chromatography. Disc-electrophoresis and especially isoelectric focusing in polyacrylamide gel showed that each fraction contained several components. Not less than 15 different components as a whole with isoelectric points ranging from 4.5 to 7.0 could be counted in four aci-GTH preparations. All these components were active in toad oocyte maturation test. Only two of four preparations (aci-GTH-A and -D) were practically free of common components. All aci-GTH preparations were shown to be homogeneous and identical by molecular weight, sedimentation coefficient, sialic acid content, and some immunological properties. <ce:italic>N</ce:italic>-terminal amino acid analysis revealed tyrosine and leucine in all aci-GTH preparations, with the only exception of aci-GTH-D that contained an additional polypeptide with <ce:italic>N</ce:italic>-terminal glycine. No differences in the spectra of aci-GTH isoforms were found when pituitary extract, newly purified or 3 years older hormone preparations were submitted to isoelectric focusing.</ce:simple-para></ce:abstract-sec></ce:abstract></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Pituitary gonadotropic hormone from a chondrostean fish, starred sturgeon ( Acipenser stellatus Pall.)</title><subTitle>III. Polymorphism</subTitle></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Pituitary gonadotropic hormone from a chondrostean fish, starred sturgeon (</title><subTitle>III. Polymorphism</subTitle></titleInfo><name type="personal"><namePart type="given">A.A.</namePart><namePart type="family">Kuznetzov</namePart><affiliation>N. K. 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Koltzov Institute of Developmental Biology, USSR Academy of Sciences, 26 Vavilov St., Moscow 177 808, USSR</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">E.</namePart><namePart type="family">Burzawa-Gerard</namePart><affiliation>Laboratoire de Physiologie Générale et comparée et Laboratoire d'Endocrinologie comparée associé au CNRS, Muséum national d'Histoire naturelle, 7 rue Cuvier, 75230 Paris Cedex 5, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article"></genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1983</dateIssued><copyrightDate encoding="w3cdtf">1983</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">Four biologically active fractions of gonadotropic hormone (aci-GTH-A, -B, -C, -D) were isolated and purified from acetonized pituitaries of the starred sturgeon (Acipenser stellatus Pall.). Their separation was achieved by DEAE-cellulose chromatography. Disc-electrophoresis and especially isoelectric focusing in polyacrylamide gel showed that each fraction contained several components. Not less than 15 different components as a whole with isoelectric points ranging from 4.5 to 7.0 could be counted in four aci-GTH preparations. All these components were active in toad oocyte maturation test. Only two of four preparations (aci-GTH-A and -D) were practically free of common components. All aci-GTH preparations were shown to be homogeneous and identical by molecular weight, sedimentation coefficient, sialic acid content, and some immunological properties. N-terminal amino acid analysis revealed tyrosine and leucine in all aci-GTH preparations, with the only exception of aci-GTH-D that contained an additional polypeptide with N-terminal glycine. No differences in the spectra of aci-GTH isoforms were found when pituitary extract, newly purified or 3 years older hormone preparations were submitted to isoelectric focusing.</abstract><relatedItem type="host"><titleInfo><title>General and Comparative Endocrinology</title></titleInfo><titleInfo type="abbreviated"><title>YGCEN</title></titleInfo><genre type="journal">journal</genre><originInfo><dateIssued encoding="w3cdtf">198303</dateIssued></originInfo><identifier type="ISSN">0016-6480</identifier><identifier type="PII">S0016-6480(00)X0248-1</identifier><part><date>198303</date><detail type="volume"><number>49</number><caption>vol.</caption></detail><detail type="issue"><number>3</number><caption>no.</caption></detail><extent unit="issue pages"><start>333</start><end>499</end></extent><extent unit="pages"><start>364</start><end>374</end></extent></part></relatedItem><identifier type="istex">2B85ACF483F193588431A7D60C0CDDCFCDECCE5A</identifier><identifier type="DOI">10.1016/0016-6480(83)90201-0</identifier><identifier type="PII">0016-6480(83)90201-0</identifier><recordInfo><recordContentSource>ELSEVIER</recordContentSource></recordInfo></mods></metadata></istex></record>Pour manipuler ce document sous Unix (Dilib)
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