Assimilation and catabolism of dispensable and indispensable free amino acids in post-larval Senegal sole ( Solea senegalensis )
Identifieur interne : 000890 ( Istex/Corpus ); précédent : 000889; suivant : 000891Assimilation and catabolism of dispensable and indispensable free amino acids in post-larval Senegal sole ( Solea senegalensis )
Auteurs : I. R Nnestad ; L. E. C Conceição ; C. Aragão ; M. T DinisSource :
- Comparative Biochemistry and Physiology [ 1532-0456 ] ; 2001.
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Abstract
The postprandial metabolism of dietary free amino acids (AA) was studied in post-larval Senegal sole, Solea senegalensis, aged 32 days after hatching (DAH). The diet was administered as a single pulse (36 nl, 43.1 mmol/l) using a micro tube-feeding technique and a dissolved mixture of crystalline AA. In four separate treatments the diet contained L [U-14C] tracer for two indispensable AA (IAA), lysine and arginine or two dispensable amino acids (DAA), glutamate and alanine. The post-larva absorbed all tested AA from the diet with similar efficiency (97.5%). A small fraction of the IAA was catabolised (11.5±1.1 and 15.1±3.3%, for lysine and arginine, respectively) and a high proportion was retained in the body (86.7±1.3 and 81.6±4.1%). For the DAA more were catabolised (64.9±5.3% and 41.4±7.2% for glutamate and alanine, respectively) and less were retained (32.9±5.1% and 56.3±7.2%). On this basis, it appears that post-larval Senegal sole use DAA in preference to IAA as energy substrates while the retention (assimilation efficiency) is better for the IAA. These results support other recent studies that early stages of fish have a better capacity to regulate AA catabolism than previously believed and that indispensable AA are saved for body growth.
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DOI: 10.1016/S1532-0456(01)00272-1
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<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Assimilation and catabolism of dispensable and indispensable free amino acids in post-larval Senegal sole ( Solea senegalensis )</title><author><name sortKey="R Nnestad, I" sort="R Nnestad, I" uniqKey="R Nnestad I" first="I" last="R Nnestad">I. R Nnestad</name><affiliation><mods:affiliation>Department of Zoology, University of Bergen, Allégt 41, N-5007 Bergen, Norway</mods:affiliation></affiliation><affiliation><mods:affiliation>Corresponding author. Tel: +47-55-58-35-86; fax: +47-55-58-96-73</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: ivar.ronnestad@zoo.uib.no</mods:affiliation></affiliation></author><author><name sortKey="Conceicao, L E C" sort="Conceicao, L E C" uniqKey="Conceicao L" first="L. E. C" last="Conceição">L. E. C Conceição</name><affiliation><mods:affiliation>CCMAR, University of Algarve, Campus de Gambelas, 8000 Faro, Portugal</mods:affiliation></affiliation></author><author><name sortKey="Aragao, C" sort="Aragao, C" uniqKey="Aragao C" first="C" last="Aragão">C. Aragão</name><affiliation><mods:affiliation>CCMAR, University of Algarve, Campus de Gambelas, 8000 Faro, Portugal</mods:affiliation></affiliation></author><author><name sortKey="Dinis, M T" sort="Dinis, M T" uniqKey="Dinis M" first="M. T" last="Dinis">M. T Dinis</name><affiliation><mods:affiliation>CCMAR, University of Algarve, Campus de Gambelas, 8000 Faro, Portugal</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:7B9F048CB9E3B356A1BF648F5396FA6687B111FA</idno><date when="2001" year="2001">2001</date><idno type="doi">10.1016/S1532-0456(01)00272-1</idno><idno type="url">https://api.istex.fr/document/7B9F048CB9E3B356A1BF648F5396FA6687B111FA/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000890</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000890</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Assimilation and catabolism of dispensable and indispensable free amino acids in post-larval Senegal sole ( Solea senegalensis )</title><author><name sortKey="R Nnestad, I" sort="R Nnestad, I" uniqKey="R Nnestad I" first="I" last="R Nnestad">I. R Nnestad</name><affiliation><mods:affiliation>Department of Zoology, University of Bergen, Allégt 41, N-5007 Bergen, Norway</mods:affiliation></affiliation><affiliation><mods:affiliation>Corresponding author. Tel: +47-55-58-35-86; fax: +47-55-58-96-73</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: ivar.ronnestad@zoo.uib.no</mods:affiliation></affiliation></author><author><name sortKey="Conceicao, L E C" sort="Conceicao, L E C" uniqKey="Conceicao L" first="L. E. C" last="Conceição">L. E. C Conceição</name><affiliation><mods:affiliation>CCMAR, University of Algarve, Campus de Gambelas, 8000 Faro, Portugal</mods:affiliation></affiliation></author><author><name sortKey="Aragao, C" sort="Aragao, C" uniqKey="Aragao C" first="C" last="Aragão">C. Aragão</name><affiliation><mods:affiliation>CCMAR, University of Algarve, Campus de Gambelas, 8000 Faro, Portugal</mods:affiliation></affiliation></author><author><name sortKey="Dinis, M T" sort="Dinis, M T" uniqKey="Dinis M" first="M. T" last="Dinis">M. T Dinis</name><affiliation><mods:affiliation>CCMAR, University of Algarve, Campus de Gambelas, 8000 Faro, Portugal</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Comparative Biochemistry and Physiology</title><title level="j" type="abbrev">CBC</title><idno type="ISSN">1532-0456</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="2001">2001</date><biblScope unit="volume">130</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="461">461</biblScope><biblScope unit="page" to="466">466</biblScope></imprint><idno type="ISSN">1532-0456</idno></series><idno type="istex">7B9F048CB9E3B356A1BF648F5396FA6687B111FA</idno><idno type="DOI">10.1016/S1532-0456(01)00272-1</idno><idno type="PII">S1532-0456(01)00272-1</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1532-0456</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Alanine</term><term>Arginine</term><term>Assimilation</term><term>Fish larvae</term><term>Free amino acids</term><term>Glutamate</term><term>Indispensable amino acids</term><term>Lysine</term><term>Oxidation</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The postprandial metabolism of dietary free amino acids (AA) was studied in post-larval Senegal sole, Solea senegalensis, aged 32 days after hatching (DAH). The diet was administered as a single pulse (36 nl, 43.1 mmol/l) using a micro tube-feeding technique and a dissolved mixture of crystalline AA. In four separate treatments the diet contained L [U-14C] tracer for two indispensable AA (IAA), lysine and arginine or two dispensable amino acids (DAA), glutamate and alanine. The post-larva absorbed all tested AA from the diet with similar efficiency (97.5%). A small fraction of the IAA was catabolised (11.5±1.1 and 15.1±3.3%, for lysine and arginine, respectively) and a high proportion was retained in the body (86.7±1.3 and 81.6±4.1%). For the DAA more were catabolised (64.9±5.3% and 41.4±7.2% for glutamate and alanine, respectively) and less were retained (32.9±5.1% and 56.3±7.2%). On this basis, it appears that post-larval Senegal sole use DAA in preference to IAA as energy substrates while the retention (assimilation efficiency) is better for the IAA. These results support other recent studies that early stages of fish have a better capacity to regulate AA catabolism than previously believed and that indispensable AA are saved for body growth.</div></front></TEI><istex><corpusName>elsevier</corpusName><author><json:item><name>I Rønnestad</name><affiliations><json:string>Department of Zoology, University of Bergen, Allégt 41, N-5007 Bergen, Norway</json:string><json:string>Corresponding author. Tel: +47-55-58-35-86; fax: +47-55-58-96-73</json:string><json:string>E-mail: ivar.ronnestad@zoo.uib.no</json:string></affiliations></json:item><json:item><name>L.E.C Conceição</name><affiliations><json:string>CCMAR, University of Algarve, Campus de Gambelas, 8000 Faro, Portugal</json:string></affiliations></json:item><json:item><name>C Aragão</name><affiliations><json:string>CCMAR, University of Algarve, Campus de Gambelas, 8000 Faro, Portugal</json:string></affiliations></json:item><json:item><name>M.T Dinis</name><affiliations><json:string>CCMAR, University of Algarve, Campus de Gambelas, 8000 Faro, Portugal</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Alanine</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Arginine</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Assimilation</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Fish larvae</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Free amino acids</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Glutamate</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Indispensable amino acids</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Lysine</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Oxidation</value></json:item></subject><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>The postprandial metabolism of dietary free amino acids (AA) was studied in post-larval Senegal sole, Solea senegalensis, aged 32 days after hatching (DAH). The diet was administered as a single pulse (36 nl, 43.1 mmol/l) using a micro tube-feeding technique and a dissolved mixture of crystalline AA. In four separate treatments the diet contained L [U-14C] tracer for two indispensable AA (IAA), lysine and arginine or two dispensable amino acids (DAA), glutamate and alanine. The post-larva absorbed all tested AA from the diet with similar efficiency (97.5%). A small fraction of the IAA was catabolised (11.5±1.1 and 15.1±3.3%, for lysine and arginine, respectively) and a high proportion was retained in the body (86.7±1.3 and 81.6±4.1%). For the DAA more were catabolised (64.9±5.3% and 41.4±7.2% for glutamate and alanine, respectively) and less were retained (32.9±5.1% and 56.3±7.2%). On this basis, it appears that post-larval Senegal sole use DAA in preference to IAA as energy substrates while the retention (assimilation efficiency) is better for the IAA. These results support other recent studies that early stages of fish have a better capacity to regulate AA catabolism than previously believed and that indispensable AA are saved for body growth.</abstract><qualityIndicators><score>5.908</score><pdfVersion>1.2</pdfVersion><pdfPageSize>595 x 794 pts</pdfPageSize><refBibsNative>true</refBibsNative><keywordCount>9</keywordCount><abstractCharCount>1265</abstractCharCount><pdfWordCount>3568</pdfWordCount><pdfCharCount>21276</pdfCharCount><pdfPageCount>6</pdfPageCount><abstractWordCount>195</abstractWordCount></qualityIndicators><title>Assimilation and catabolism of dispensable and indispensable free amino acids in post-larval Senegal sole ( Solea senegalensis )</title><pii><json:string>S1532-0456(01)00272-1</json:string></pii><genre><json:string>research-article</json:string></genre><serie><volume>vol. VIII</volume><editor><json:item><name>W.S. Hoar</name></json:item><json:item><name>D.J. Randall</name></json:item><json:item><name>J.R. Brett</name></json:item></editor><pages><last>70</last><first>1</first></pages><language><json:string>unknown</json:string></language><title>Fish Physiology</title></serie><host><volume>130</volume><pii><json:string>S1532-0456(00)X0012-9</json:string></pii><pages><last>466</last><first>461</first></pages><issn><json:string>1532-0456</json:string></issn><issue>4</issue><genre><json:string>journal</json:string></genre><language><json:string>unknown</json:string></language><title>Comparative Biochemistry and Physiology</title><publicationDate>2001</publicationDate></host><categories><wos><json:string>science</json:string><json:string>zoology</json:string><json:string>toxicology</json:string><json:string>endocrinology & metabolism</json:string><json:string>biochemistry & molecular biology</json:string></wos><scienceMetrix><json:string>health sciences</json:string><json:string>biomedical research</json:string><json:string>toxicology</json:string></scienceMetrix></categories><publicationDate>2001</publicationDate><copyrightDate>2001</copyrightDate><doi><json:string>10.1016/S1532-0456(01)00272-1</json:string></doi><id>7B9F048CB9E3B356A1BF648F5396FA6687B111FA</id><score>0.036207322</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/document/7B9F048CB9E3B356A1BF648F5396FA6687B111FA/fulltext/pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/document/7B9F048CB9E3B356A1BF648F5396FA6687B111FA/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/7B9F048CB9E3B356A1BF648F5396FA6687B111FA/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Assimilation and catabolism of dispensable and indispensable free amino acids in post-larval Senegal sole ( Solea senegalensis )</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>ELSEVIER</publisher><availability><p>©2001 Elsevier Science Inc.</p></availability><date>2001</date></publicationStmt><notesStmt><note>This paper was originally presented at a symposium dedicated to the memory of Marcel Florkin, held within the ESCPB 21st International Congress, Liège, Belgium, July 24–28, 2000.</note><note type="content">Fig. 1: Proportion (% of total tracer) of the tube-fed 14C label found in each compartment in post-larval Senegal sole, Solea senegalensis (11.2±2.0 mg wet wt., 32 days post hatch) for the indispensable (lysine, arginine) and dispensable (glutamate, alanine) amino acid diet treatments. Values are means (±S.E.M.) of 8–10 larvae. Values with different letters in the same compartment are significantly different (ANOVA, followed by Bonferroni t-test, P<0.005).</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Assimilation and catabolism of dispensable and indispensable free amino acids in post-larval Senegal sole ( Solea senegalensis )</title><author xml:id="author-1"><persName><forename type="first">I</forename><surname>Rønnestad</surname></persName><email>ivar.ronnestad@zoo.uib.no</email><affiliation>Department of Zoology, University of Bergen, Allégt 41, N-5007 Bergen, Norway</affiliation><affiliation>Corresponding author. Tel: +47-55-58-35-86; fax: +47-55-58-96-73</affiliation></author><author xml:id="author-2"><persName><forename type="first">L.E.C</forename><surname>Conceição</surname></persName><affiliation>CCMAR, University of Algarve, Campus de Gambelas, 8000 Faro, Portugal</affiliation></author><author xml:id="author-3"><persName><forename type="first">C</forename><surname>Aragão</surname></persName><affiliation>CCMAR, University of Algarve, Campus de Gambelas, 8000 Faro, Portugal</affiliation></author><author xml:id="author-4"><persName><forename type="first">M.T</forename><surname>Dinis</surname></persName><affiliation>CCMAR, University of Algarve, Campus de Gambelas, 8000 Faro, Portugal</affiliation></author></analytic><monogr><title level="j">Comparative Biochemistry and Physiology</title><title level="j" type="abbrev">CBC</title><idno type="pISSN">1532-0456</idno><idno type="PII">S1532-0456(00)X0012-9</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="2001"></date><biblScope unit="volume">130</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="461">461</biblScope><biblScope unit="page" to="466">466</biblScope></imprint></monogr><idno type="istex">7B9F048CB9E3B356A1BF648F5396FA6687B111FA</idno><idno type="DOI">10.1016/S1532-0456(01)00272-1</idno><idno type="PII">S1532-0456(01)00272-1</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2001</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>The postprandial metabolism of dietary free amino acids (AA) was studied in post-larval Senegal sole, Solea senegalensis, aged 32 days after hatching (DAH). The diet was administered as a single pulse (36 nl, 43.1 mmol/l) using a micro tube-feeding technique and a dissolved mixture of crystalline AA. In four separate treatments the diet contained L [U-14C] tracer for two indispensable AA (IAA), lysine and arginine or two dispensable amino acids (DAA), glutamate and alanine. The post-larva absorbed all tested AA from the diet with similar efficiency (97.5%). A small fraction of the IAA was catabolised (11.5±1.1 and 15.1±3.3%, for lysine and arginine, respectively) and a high proportion was retained in the body (86.7±1.3 and 81.6±4.1%). For the DAA more were catabolised (64.9±5.3% and 41.4±7.2% for glutamate and alanine, respectively) and less were retained (32.9±5.1% and 56.3±7.2%). On this basis, it appears that post-larval Senegal sole use DAA in preference to IAA as energy substrates while the retention (assimilation efficiency) is better for the IAA. These results support other recent studies that early stages of fish have a better capacity to regulate AA catabolism than previously believed and that indispensable AA are saved for body growth.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>Keywords</head><item><term>Alanine</term></item><item><term>Arginine</term></item><item><term>Assimilation</term></item><item><term>Fish larvae</term></item><item><term>Free amino acids</term></item><item><term>Glutamate</term></item><item><term>Indispensable amino acids</term></item><item><term>Lysine</term></item><item><term>Oxidation</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2001-04-11">Modified</change><change when="2001">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/7B9F048CB9E3B356A1BF648F5396FA6687B111FA/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: ce:floats; body; tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"><istex:entity SYSTEM="gr1" NDATA="IMAGE" name="gr1"></istex:entity></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla" xml:lang="en"><item-info><jid>CBC</jid><aid>6455</aid><ce:pii>S1532-0456(01)00272-1</ce:pii><ce:doi>10.1016/S1532-0456(01)00272-1</ce:doi><ce:copyright type="full-transfer" year="2001">Elsevier Science Inc.</ce:copyright></item-info><head><ce:article-footnote><ce:label>☆</ce:label><ce:note-para>This paper was originally presented at a symposium dedicated to the memory of Marcel Florkin, held within the ESCPB 21st International Congress, Liège, Belgium, July 24–28, 2000.</ce:note-para></ce:article-footnote><ce:title>Assimilation and catabolism of dispensable and indispensable free amino acids in post-larval Senegal sole (<ce:italic>Solea senegalensis</ce:italic>)</ce:title><ce:author-group><ce:author><ce:given-name>I</ce:given-name><ce:surname>Rønnestad</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>a</ce:sup></ce:cross-ref><ce:cross-ref refid="COR1">*</ce:cross-ref><ce:e-address type="email">ivar.ronnestad@zoo.uib.no</ce:e-address></ce:author><ce:author><ce:given-name>L.E.C</ce:given-name><ce:surname>Conceição</ce:surname><ce:cross-ref refid="AFF2"><ce:sup>b</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>C</ce:given-name><ce:surname>Aragão</ce:surname><ce:cross-ref refid="AFF2"><ce:sup>b</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>M.T</ce:given-name><ce:surname>Dinis</ce:surname><ce:cross-ref refid="AFF2"><ce:sup>b</ce:sup></ce:cross-ref></ce:author><ce:affiliation id="AFF1"><ce:label>a</ce:label><ce:textfn>Department of Zoology, University of Bergen, Allégt 41, N-5007 Bergen, Norway</ce:textfn></ce:affiliation><ce:affiliation id="AFF2"><ce:label>b</ce:label><ce:textfn>CCMAR, University of Algarve, Campus de Gambelas, 8000 Faro, Portugal</ce:textfn></ce:affiliation><ce:correspondence id="COR1"><ce:label>*</ce:label><ce:text>Corresponding author. Tel: +47-55-58-35-86; fax: +47-55-58-96-73</ce:text></ce:correspondence></ce:author-group><ce:date-received day="22" month="12" year="2000"></ce:date-received><ce:date-revised day="11" month="4" year="2001"></ce:date-revised><ce:date-accepted day="17" month="4" year="2001"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>The postprandial metabolism of dietary free amino acids (AA) was studied in post-larval Senegal sole, <ce:italic>Solea senegalensis</ce:italic>, aged 32 days after hatching (DAH). The diet was administered as a single pulse (36 nl, 43.1 mmol/l) using a micro tube-feeding technique and a dissolved mixture of crystalline AA. In four separate treatments the diet contained L [U-<ce:sup>14</ce:sup>C] tracer for two indispensable AA (IAA), lysine and arginine or two dispensable amino acids (DAA), glutamate and alanine. The post-larva absorbed all tested AA from the diet with similar efficiency (97.5%). A small fraction of the IAA was catabolised (11.5±1.1 and 15.1±3.3%, for lysine and arginine, respectively) and a high proportion was retained in the body (86.7±1.3 and 81.6±4.1%). For the DAA more were catabolised (64.9±5.3% and 41.4±7.2% for glutamate and alanine, respectively) and less were retained (32.9±5.1% and 56.3±7.2%). On this basis, it appears that post-larval Senegal sole use DAA in preference to IAA as energy substrates while the retention (assimilation efficiency) is better for the IAA. These results support other recent studies that early stages of fish have a better capacity to regulate AA catabolism than previously believed and that indispensable AA are saved for body growth.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords class="keyword"><ce:section-title>Keywords</ce:section-title><ce:keyword><ce:text>Alanine</ce:text></ce:keyword><ce:keyword><ce:text>Arginine</ce:text></ce:keyword><ce:keyword><ce:text>Assimilation</ce:text></ce:keyword><ce:keyword><ce:text>Fish larvae</ce:text></ce:keyword><ce:keyword><ce:text>Free amino acids</ce:text></ce:keyword><ce:keyword><ce:text>Glutamate</ce:text></ce:keyword><ce:keyword><ce:text>Indispensable amino acids</ce:text></ce:keyword><ce:keyword><ce:text>Lysine</ce:text></ce:keyword><ce:keyword><ce:text>Oxidation</ce:text></ce:keyword></ce:keywords></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Assimilation and catabolism of dispensable and indispensable free amino acids in post-larval Senegal sole ( Solea senegalensis )</title></titleInfo><titleInfo type="alternative" lang="en" contentType="CDATA"><title>Assimilation and catabolism of dispensable and indispensable free amino acids in post-larval Senegal sole (</title></titleInfo><name type="personal"><namePart type="given">I</namePart><namePart type="family">Rønnestad</namePart><affiliation>Department of Zoology, University of Bergen, Allégt 41, N-5007 Bergen, Norway</affiliation><affiliation>Corresponding author. Tel: +47-55-58-35-86; fax: +47-55-58-96-73</affiliation><affiliation>E-mail: ivar.ronnestad@zoo.uib.no</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">L.E.C</namePart><namePart type="family">Conceição</namePart><affiliation>CCMAR, University of Algarve, Campus de Gambelas, 8000 Faro, Portugal</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">C</namePart><namePart type="family">Aragão</namePart><affiliation>CCMAR, University of Algarve, Campus de Gambelas, 8000 Faro, Portugal</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.T</namePart><namePart type="family">Dinis</namePart><affiliation>CCMAR, University of Algarve, Campus de Gambelas, 8000 Faro, Portugal</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article"></genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">2001</dateIssued><dateModified encoding="w3cdtf">2001-04-11</dateModified><copyrightDate encoding="w3cdtf">2001</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">The postprandial metabolism of dietary free amino acids (AA) was studied in post-larval Senegal sole, Solea senegalensis, aged 32 days after hatching (DAH). The diet was administered as a single pulse (36 nl, 43.1 mmol/l) using a micro tube-feeding technique and a dissolved mixture of crystalline AA. In four separate treatments the diet contained L [U-14C] tracer for two indispensable AA (IAA), lysine and arginine or two dispensable amino acids (DAA), glutamate and alanine. The post-larva absorbed all tested AA from the diet with similar efficiency (97.5%). A small fraction of the IAA was catabolised (11.5±1.1 and 15.1±3.3%, for lysine and arginine, respectively) and a high proportion was retained in the body (86.7±1.3 and 81.6±4.1%). For the DAA more were catabolised (64.9±5.3% and 41.4±7.2% for glutamate and alanine, respectively) and less were retained (32.9±5.1% and 56.3±7.2%). On this basis, it appears that post-larval Senegal sole use DAA in preference to IAA as energy substrates while the retention (assimilation efficiency) is better for the IAA. These results support other recent studies that early stages of fish have a better capacity to regulate AA catabolism than previously believed and that indispensable AA are saved for body growth.</abstract><note>This paper was originally presented at a symposium dedicated to the memory of Marcel Florkin, held within the ESCPB 21st International Congress, Liège, Belgium, July 24–28, 2000.</note><note type="content">Fig. 1: Proportion (% of total tracer) of the tube-fed 14C label found in each compartment in post-larval Senegal sole, Solea senegalensis (11.2±2.0 mg wet wt., 32 days post hatch) for the indispensable (lysine, arginine) and dispensable (glutamate, alanine) amino acid diet treatments. Values are means (±S.E.M.) of 8–10 larvae. Values with different letters in the same compartment are significantly different (ANOVA, followed by Bonferroni t-test, P<0.005).</note><subject lang="en"><genre>Keywords</genre><topic>Alanine</topic><topic>Arginine</topic><topic>Assimilation</topic><topic>Fish larvae</topic><topic>Free amino acids</topic><topic>Glutamate</topic><topic>Indispensable amino acids</topic><topic>Lysine</topic><topic>Oxidation</topic></subject><relatedItem type="host"><titleInfo><title>Comparative Biochemistry and Physiology</title></titleInfo><titleInfo type="abbreviated"><title>CBC</title></titleInfo><genre type="journal">journal</genre><originInfo><dateIssued encoding="w3cdtf">200112</dateIssued></originInfo><identifier type="ISSN">1532-0456</identifier><identifier type="PII">S1532-0456(00)X0012-9</identifier><part><date>200112</date><detail type="volume"><number>130</number><caption>vol.</caption></detail><detail type="issue"><number>4</number><caption>no.</caption></detail><extent unit="issue pages"><start>397</start><end>488</end></extent><extent unit="pages"><start>461</start><end>466</end></extent></part></relatedItem><identifier type="istex">7B9F048CB9E3B356A1BF648F5396FA6687B111FA</identifier><identifier type="DOI">10.1016/S1532-0456(01)00272-1</identifier><identifier type="PII">S1532-0456(01)00272-1</identifier><accessCondition type="use and reproduction" contentType="copyright">©2001 Elsevier Science Inc.</accessCondition><recordInfo><recordContentSource>ELSEVIER</recordContentSource><recordOrigin>Elsevier Science Inc., ©2001</recordOrigin></recordInfo></mods></metadata></istex></record>Pour manipuler ce document sous Unix (Dilib)
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