Development of two cell culture systems from Asian seabass Lates calcarifer (Bloch)
Identifieur interne : 000888 ( Istex/Corpus ); précédent : 000887; suivant : 000889Development of two cell culture systems from Asian seabass Lates calcarifer (Bloch)
Auteurs : Wazir S. Lakra ; Nachimuthu Sivakumar ; Mukunda Goswami ; Ramesh R. BhondeSource :
- Aquaculture Research [ 1355-557X ] ; 2006-01.
English descriptors
- KwdEn :
Abstract
Two new cell culture systems namely epitheloid cells of Lates (LCE) and fibroblastic cells of Lates (LCF) have been developed from fry and fingerling of the economically important brackishwater fish Lates calcarifer. Primary cultures were initiated by explant technique using caudal fin of fingerling and whole body tissue of the fry. The nutritional requirements and the growth pattern in response to different culture environment were similar for the two cell cultures. The culture medium used was Leibovitz‐15 supplemented with 20% fetal bovine serum (FBS) and 1% fish serum. The LCE comprised of epithelioid cells and LCF cells were fibroblastic. With a split ratio of 1:2, the confluency of cells was attained in 8–10 days at an incubation temperature of 28°C. The cells were found to grow well in a wide range of temperature (24–32°C) and stable at 20 and 36°C. The growth rate of LCF and LCE cells increased proportionately with the concentration of FBS from 5% to 20%. A decrease of serum level to 10% after eight subcultures produced no apparent change in cell morphology and growth rate. The viability of cells was found to be 70% when revived after a month of storage in liquid nitrogen (−196°C).
Url:
DOI: 10.1111/j.1365-2109.2005.01387.x
Links to Exploration step
ISTEX:D17EAD84594E94A3C73C44DCB4BF4AD640FDED5ELe document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Development of two cell culture systems from Asian seabass Lates calcarifer (Bloch)</title><author><name sortKey="Lakra, Wazir S" sort="Lakra, Wazir S" uniqKey="Lakra W" first="Wazir S" last="Lakra">Wazir S. Lakra</name><affiliation><mods:affiliation>Central Institute of Fisheries Education, Versova, Mumbai, India</mods:affiliation></affiliation></author><author><name sortKey="Sivakumar, Nachimuthu" sort="Sivakumar, Nachimuthu" uniqKey="Sivakumar N" first="Nachimuthu" last="Sivakumar">Nachimuthu Sivakumar</name><affiliation><mods:affiliation>Central Institute of Fisheries Education, Versova, Mumbai, India</mods:affiliation></affiliation></author><author><name sortKey="Goswami, Mukunda" sort="Goswami, Mukunda" uniqKey="Goswami M" first="Mukunda" last="Goswami">Mukunda Goswami</name><affiliation><mods:affiliation>Central Institute of Fisheries Education, Versova, Mumbai, India</mods:affiliation></affiliation></author><author><name sortKey="Bhonde, Ramesh R" sort="Bhonde, Ramesh R" uniqKey="Bhonde R" first="Ramesh R" last="Bhonde">Ramesh R. Bhonde</name><affiliation><mods:affiliation>National Centre for Cell Science, Ganeshkhind, Pune, India</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:D17EAD84594E94A3C73C44DCB4BF4AD640FDED5E</idno><date when="2006" year="2006">2006</date><idno type="doi">10.1111/j.1365-2109.2005.01387.x</idno><idno type="url">https://api.istex.fr/document/D17EAD84594E94A3C73C44DCB4BF4AD640FDED5E/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000888</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000888</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Development of two cell culture systems from Asian seabass Lates calcarifer (Bloch)</title><author><name sortKey="Lakra, Wazir S" sort="Lakra, Wazir S" uniqKey="Lakra W" first="Wazir S" last="Lakra">Wazir S. Lakra</name><affiliation><mods:affiliation>Central Institute of Fisheries Education, Versova, Mumbai, India</mods:affiliation></affiliation></author><author><name sortKey="Sivakumar, Nachimuthu" sort="Sivakumar, Nachimuthu" uniqKey="Sivakumar N" first="Nachimuthu" last="Sivakumar">Nachimuthu Sivakumar</name><affiliation><mods:affiliation>Central Institute of Fisheries Education, Versova, Mumbai, India</mods:affiliation></affiliation></author><author><name sortKey="Goswami, Mukunda" sort="Goswami, Mukunda" uniqKey="Goswami M" first="Mukunda" last="Goswami">Mukunda Goswami</name><affiliation><mods:affiliation>Central Institute of Fisheries Education, Versova, Mumbai, India</mods:affiliation></affiliation></author><author><name sortKey="Bhonde, Ramesh R" sort="Bhonde, Ramesh R" uniqKey="Bhonde R" first="Ramesh R" last="Bhonde">Ramesh R. Bhonde</name><affiliation><mods:affiliation>National Centre for Cell Science, Ganeshkhind, Pune, India</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Aquaculture Research</title><idno type="ISSN">1355-557X</idno><idno type="eISSN">1365-2109</idno><imprint><publisher>Blackwell Science Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2006-01">2006-01</date><biblScope unit="volume">37</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="18">18</biblScope><biblScope unit="page" to="24">24</biblScope></imprint><idno type="ISSN">1355-557X</idno></series><idno type="istex">D17EAD84594E94A3C73C44DCB4BF4AD640FDED5E</idno><idno type="DOI">10.1111/j.1365-2109.2005.01387.x</idno><idno type="ArticleID">ARE1387</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1355-557X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>LCE</term><term>LCF</term><term>cell cultures</term><term>growth studies</term><term>seabass</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Two new cell culture systems namely epitheloid cells of Lates (LCE) and fibroblastic cells of Lates (LCF) have been developed from fry and fingerling of the economically important brackishwater fish Lates calcarifer. Primary cultures were initiated by explant technique using caudal fin of fingerling and whole body tissue of the fry. The nutritional requirements and the growth pattern in response to different culture environment were similar for the two cell cultures. The culture medium used was Leibovitz‐15 supplemented with 20% fetal bovine serum (FBS) and 1% fish serum. The LCE comprised of epithelioid cells and LCF cells were fibroblastic. With a split ratio of 1:2, the confluency of cells was attained in 8–10 days at an incubation temperature of 28°C. The cells were found to grow well in a wide range of temperature (24–32°C) and stable at 20 and 36°C. The growth rate of LCF and LCE cells increased proportionately with the concentration of FBS from 5% to 20%. A decrease of serum level to 10% after eight subcultures produced no apparent change in cell morphology and growth rate. The viability of cells was found to be 70% when revived after a month of storage in liquid nitrogen (−196°C).</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>Wazir S Lakra</name><affiliations><json:string>Central Institute of Fisheries Education, Versova, Mumbai, India</json:string></affiliations></json:item><json:item><name>Nachimuthu Sivakumar</name><affiliations><json:string>Central Institute of Fisheries Education, Versova, Mumbai, India</json:string></affiliations></json:item><json:item><name>Mukunda Goswami</name><affiliations><json:string>Central Institute of Fisheries Education, Versova, Mumbai, India</json:string></affiliations></json:item><json:item><name>Ramesh R Bhonde</name><affiliations><json:string>National Centre for Cell Science, Ganeshkhind, Pune, India</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>seabass</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>cell cultures</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>LCF</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>LCE</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>growth studies</value></json:item></subject><articleId><json:string>ARE1387</json:string></articleId><language><json:string>eng</json:string></language><originalGenre><json:string>article</json:string></originalGenre><abstract>Two new cell culture systems namely epitheloid cells of Lates (LCE) and fibroblastic cells of Lates (LCF) have been developed from fry and fingerling of the economically important brackishwater fish Lates calcarifer. Primary cultures were initiated by explant technique using caudal fin of fingerling and whole body tissue of the fry. The nutritional requirements and the growth pattern in response to different culture environment were similar for the two cell cultures. The culture medium used was Leibovitz‐15 supplemented with 20% fetal bovine serum (FBS) and 1% fish serum. The LCE comprised of epithelioid cells and LCF cells were fibroblastic. With a split ratio of 1:2, the confluency of cells was attained in 8–10 days at an incubation temperature of 28°C. The cells were found to grow well in a wide range of temperature (24–32°C) and stable at 20 and 36°C. The growth rate of LCF and LCE cells increased proportionately with the concentration of FBS from 5% to 20%. A decrease of serum level to 10% after eight subcultures produced no apparent change in cell morphology and growth rate. The viability of cells was found to be 70% when revived after a month of storage in liquid nitrogen (−196°C).</abstract><qualityIndicators><score>5.9</score><pdfVersion>1.3</pdfVersion><pdfPageSize>595.276 x 782.362 pts</pdfPageSize><refBibsNative>true</refBibsNative><abstractCharCount>1207</abstractCharCount><pdfWordCount>3512</pdfWordCount><pdfCharCount>20733</pdfCharCount><pdfPageCount>7</pdfPageCount><abstractWordCount>199</abstractWordCount></qualityIndicators><title>Development of two cell culture systems from Asian seabass Lates calcarifer (Bloch)</title><genre><json:string>article</json:string></genre><host><volume>37</volume><publisherId><json:string>ARE</json:string></publisherId><pages><total>7</total><last>24</last><first>18</first></pages><issn><json:string>1355-557X</json:string></issn><issue>1</issue><genre><json:string>journal</json:string></genre><language><json:string>unknown</json:string></language><eissn><json:string>1365-2109</json:string></eissn><title>Aquaculture Research</title><doi><json:string>10.1111/(ISSN)1365-2109</json:string></doi></host><categories><wos><json:string>science</json:string><json:string>fisheries</json:string></wos><scienceMetrix><json:string>applied sciences</json:string><json:string>agriculture, fisheries & forestry</json:string><json:string>fisheries</json:string></scienceMetrix></categories><publicationDate>2006</publicationDate><copyrightDate>2006</copyrightDate><doi><json:string>10.1111/j.1365-2109.2005.01387.x</json:string></doi><id>D17EAD84594E94A3C73C44DCB4BF4AD640FDED5E</id><score>0.02786684</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/document/D17EAD84594E94A3C73C44DCB4BF4AD640FDED5E/fulltext/pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/document/D17EAD84594E94A3C73C44DCB4BF4AD640FDED5E/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/D17EAD84594E94A3C73C44DCB4BF4AD640FDED5E/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Development of two cell culture systems from Asian seabass Lates calcarifer (Bloch)</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>Blackwell Science Ltd</publisher><pubPlace>Oxford, UK</pubPlace><availability><p>WILEY</p></availability><date>2006</date></publicationStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Development of two cell culture systems from Asian seabass Lates calcarifer (Bloch)</title><author xml:id="author-1"><persName><forename type="first">Wazir S</forename><surname>Lakra</surname></persName><affiliation>Central Institute of Fisheries Education, Versova, Mumbai, India</affiliation></author><author xml:id="author-2"><persName><forename type="first">Nachimuthu</forename><surname>Sivakumar</surname></persName><affiliation>Central Institute of Fisheries Education, Versova, Mumbai, India</affiliation></author><author xml:id="author-3"><persName><forename type="first">Mukunda</forename><surname>Goswami</surname></persName><affiliation>Central Institute of Fisheries Education, Versova, Mumbai, India</affiliation></author><author xml:id="author-4"><persName><forename type="first">Ramesh R</forename><surname>Bhonde</surname></persName><affiliation>National Centre for Cell Science, Ganeshkhind, Pune, India</affiliation></author></analytic><monogr><title level="j">Aquaculture Research</title><idno type="pISSN">1355-557X</idno><idno type="eISSN">1365-2109</idno><idno type="DOI">10.1111/(ISSN)1365-2109</idno><imprint><publisher>Blackwell Science Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2006-01"></date><biblScope unit="volume">37</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="18">18</biblScope><biblScope unit="page" to="24">24</biblScope></imprint></monogr><idno type="istex">D17EAD84594E94A3C73C44DCB4BF4AD640FDED5E</idno><idno type="DOI">10.1111/j.1365-2109.2005.01387.x</idno><idno type="ArticleID">ARE1387</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2006</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Two new cell culture systems namely epitheloid cells of Lates (LCE) and fibroblastic cells of Lates (LCF) have been developed from fry and fingerling of the economically important brackishwater fish Lates calcarifer. Primary cultures were initiated by explant technique using caudal fin of fingerling and whole body tissue of the fry. The nutritional requirements and the growth pattern in response to different culture environment were similar for the two cell cultures. The culture medium used was Leibovitz‐15 supplemented with 20% fetal bovine serum (FBS) and 1% fish serum. The LCE comprised of epithelioid cells and LCF cells were fibroblastic. With a split ratio of 1:2, the confluency of cells was attained in 8–10 days at an incubation temperature of 28°C. The cells were found to grow well in a wide range of temperature (24–32°C) and stable at 20 and 36°C. The growth rate of LCF and LCE cells increased proportionately with the concentration of FBS from 5% to 20%. A decrease of serum level to 10% after eight subcultures produced no apparent change in cell morphology and growth rate. The viability of cells was found to be 70% when revived after a month of storage in liquid nitrogen (−196°C).</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>keywords</head><item><term>seabass</term></item><item><term>cell cultures</term></item><item><term>LCF</term></item><item><term>LCE</term></item><item><term>growth studies</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2006-01">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/D17EAD84594E94A3C73C44DCB4BF4AD640FDED5E/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>Blackwell Science Ltd</publisherName><publisherLoc>Oxford, UK</publisherLoc></publisherInfo><doi origin="wiley" registered="yes">10.1111/(ISSN)1365-2109</doi><issn type="print">1355-557X</issn><issn type="electronic">1365-2109</issn><idGroup><id type="product" value="ARE"></id><id type="publisherDivision" value="ST"></id></idGroup><titleGroup><title type="main" sort="AQUACULTURE RESEARCH">Aquaculture Research</title></titleGroup></publicationMeta><publicationMeta level="part" position="01001"><doi origin="wiley">10.1111/are.2006.37.issue-1</doi><numberingGroup><numbering type="journalVolume" number="37">37</numbering><numbering type="journalIssue" number="1">1</numbering></numberingGroup><coverDate startDate="2006-01">January 2006</coverDate></publicationMeta><publicationMeta level="unit" type="article" position="2" status="forIssue"><doi origin="wiley">10.1111/j.1365-2109.2005.01387.x</doi><idGroup><id type="unit" value="ARE1387"></id><id type="supplier" value="1387"></id></idGroup><countGroup><count type="pageTotal" number="7"></count></countGroup><titleGroup><title type="tocHeading1">Original Articles</title></titleGroup><eventGroup><event type="firstOnline" date="2005-10-26"></event><event type="publishedOnlineFinalForm" date="2005-10-26"></event><event type="xmlConverted" agent="Converter:BPG_TO_WML3G version:2.3.2 mode:FullText source:FullText result:FullText" date="2010-03-16"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:3.8.8" date="2014-01-06"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-10-15"></event></eventGroup><numberingGroup><numbering type="pageFirst" number="18">18</numbering><numbering type="pageLast" number="24">24</numbering></numberingGroup><correspondenceTo><b>Correspondence:</b> Dr. W S Lakra, National Bureau of Fish Genetic Resources, Canal Ring Road, Dilkhusha (PO) Lucknow‐226002, India. E‐mail: <email normalForm="lakraws@hotmail.com">lakraws@hotmail.com</email></correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:ARE.ARE1387.pdf"></link></linkGroup></publicationMeta><contentMeta><countGroup><count type="figureTotal" number="7"></count><count type="tableTotal" number="0"></count><count type="formulaTotal" number="0"></count><count type="referenceTotal" number="31"></count><count type="wordTotal" number="4280"></count><count type="linksCrossRef" number="41"></count></countGroup><titleGroup><title type="main">Development of two cell culture systems from Asian seabass <i>Lates calcarifer</i> (Bloch)</title><title type="shortAuthors">W S Lakra <i>et al.</i></title><title type="short">Cell culture systems from <i>Lates calcarifer</i></title></titleGroup><creators><creator creatorRole="author" xml:id="cr1" affiliationRef="#a1"><personName><givenNames>Wazir S</givenNames><familyName>Lakra</familyName></personName></creator><creator creatorRole="author" xml:id="cr2" affiliationRef="#a1"><personName><givenNames>Nachimuthu</givenNames><familyName>Sivakumar</familyName></personName></creator><creator creatorRole="author" xml:id="cr3" affiliationRef="#a1"><personName><givenNames>Mukunda</givenNames><familyName>Goswami</familyName></personName></creator><creator creatorRole="author" xml:id="cr4" affiliationRef="#a2"><personName><givenNames>Ramesh R</givenNames><familyName>Bhonde</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="a1" countryCode="IN"><unparsedAffiliation>Central Institute of Fisheries Education, Versova, Mumbai, India</unparsedAffiliation></affiliation><affiliation xml:id="a2" countryCode="IN"><unparsedAffiliation>National Centre for Cell Science, Ganeshkhind, Pune, India</unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en"><keyword xml:id="k1">seabass</keyword><keyword xml:id="k2">cell cultures</keyword><keyword xml:id="k3">LCF</keyword><keyword xml:id="k4">LCE</keyword><keyword xml:id="k5">growth studies</keyword></keywordGroup><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Abstract</title><p>Two new cell culture systems namely epitheloid cells of <i>Lates</i> (LCE) and fibroblastic cells of <i>Lates</i> (LCF) have been developed from fry and fingerling of the economically important brackishwater fish <i>Lates calcarifer</i>. Primary cultures were initiated by explant technique using caudal fin of fingerling and whole body tissue of the fry. The nutritional requirements and the growth pattern in response to different culture environment were similar for the two cell cultures. The culture medium used was Leibovitz‐15 supplemented with 20% fetal bovine serum (FBS) and 1% fish serum. The LCE comprised of epithelioid cells and LCF cells were fibroblastic. With a split ratio of 1:2, the confluency of cells was attained in 8–10 days at an incubation temperature of 28°C. The cells were found to grow well in a wide range of temperature (24–32°C) and stable at 20 and 36°C. The growth rate of LCF and LCE cells increased proportionately with the concentration of FBS from 5% to 20%. A decrease of serum level to 10% after eight subcultures produced no apparent change in cell morphology and growth rate. The viability of cells was found to be 70% when revived after a month of storage in liquid nitrogen (−196°C).</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Development of two cell culture systems from Asian seabass Lates calcarifer (Bloch)</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>Cell culture systems from Lates calcarifer</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Development of two cell culture systems from Asian seabass Lates calcarifer (Bloch)</title></titleInfo><name type="personal"><namePart type="given">Wazir S</namePart><namePart type="family">Lakra</namePart><affiliation>Central Institute of Fisheries Education, Versova, Mumbai, India</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Nachimuthu</namePart><namePart type="family">Sivakumar</namePart><affiliation>Central Institute of Fisheries Education, Versova, Mumbai, India</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Mukunda</namePart><namePart type="family">Goswami</namePart><affiliation>Central Institute of Fisheries Education, Versova, Mumbai, India</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Ramesh R</namePart><namePart type="family">Bhonde</namePart><affiliation>National Centre for Cell Science, Ganeshkhind, Pune, India</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Blackwell Science Ltd</publisher><place><placeTerm type="text">Oxford, UK</placeTerm></place><dateIssued encoding="w3cdtf">2006-01</dateIssued><copyrightDate encoding="w3cdtf">2006</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">7</extent><extent unit="references">31</extent><extent unit="words">4280</extent></physicalDescription><abstract lang="en">Two new cell culture systems namely epitheloid cells of Lates (LCE) and fibroblastic cells of Lates (LCF) have been developed from fry and fingerling of the economically important brackishwater fish Lates calcarifer. Primary cultures were initiated by explant technique using caudal fin of fingerling and whole body tissue of the fry. The nutritional requirements and the growth pattern in response to different culture environment were similar for the two cell cultures. The culture medium used was Leibovitz‐15 supplemented with 20% fetal bovine serum (FBS) and 1% fish serum. The LCE comprised of epithelioid cells and LCF cells were fibroblastic. With a split ratio of 1:2, the confluency of cells was attained in 8–10 days at an incubation temperature of 28°C. The cells were found to grow well in a wide range of temperature (24–32°C) and stable at 20 and 36°C. The growth rate of LCF and LCE cells increased proportionately with the concentration of FBS from 5% to 20%. A decrease of serum level to 10% after eight subcultures produced no apparent change in cell morphology and growth rate. The viability of cells was found to be 70% when revived after a month of storage in liquid nitrogen (−196°C).</abstract><subject lang="en"><genre>keywords</genre><topic>seabass</topic><topic>cell cultures</topic><topic>LCF</topic><topic>LCE</topic><topic>growth studies</topic></subject><relatedItem type="host"><titleInfo><title>Aquaculture Research</title></titleInfo><genre type="journal">journal</genre><identifier type="ISSN">1355-557X</identifier><identifier type="eISSN">1365-2109</identifier><identifier type="DOI">10.1111/(ISSN)1365-2109</identifier><identifier type="PublisherID">ARE</identifier><part><date>2006</date><detail type="volume"><caption>vol.</caption><number>37</number></detail><detail type="issue"><caption>no.</caption><number>1</number></detail><extent unit="pages"><start>18</start><end>24</end><total>7</total></extent></part></relatedItem><identifier type="istex">D17EAD84594E94A3C73C44DCB4BF4AD640FDED5E</identifier><identifier type="DOI">10.1111/j.1365-2109.2005.01387.x</identifier><identifier type="ArticleID">ARE1387</identifier><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>Blackwell Science Ltd</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Eau/explor/EsturgeonV1/Data/Istex/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000888 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Corpus/biblio.hfd -nk 000888 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Eau
|area= EsturgeonV1
|flux= Istex
|étape= Corpus
|type= RBID
|clé= ISTEX:D17EAD84594E94A3C73C44DCB4BF4AD640FDED5E
|texte= Development of two cell culture systems from Asian seabass Lates calcarifer (Bloch)
}}
| This area was generated with Dilib version V0.6.27. |  |