Magnitude of the Root effect in red blood cells and haemoglobin solutions of fishes: a tribute to August Krogh
Identifieur interne : 000820 ( Istex/Corpus ); précédent : 000819; suivant : 000821Magnitude of the Root effect in red blood cells and haemoglobin solutions of fishes: a tribute to August Krogh
Auteurs : M. Berenbrink ; P. Koldkj R ; E. Hannah Wright ; O. Kepp ; A. José Da SilvaSource :
- Acta Physiologica [ 1748-1708 ] ; 2011-07.
English descriptors
- KwdEn :
Abstract
Aim: The ability of high carbon dioxide tensions or low pH to reduce blood oxygen binding even at high oxygen tensions, first observed by August Krogh and Isabella Leitch in 1919 and now known as the Root effect, was studied in red blood cells and haemoglobin solutions of several fish species.
Url:
DOI: 10.1111/j.1748-1716.2010.02243.x
Links to Exploration step
ISTEX:C22E76D81CBA54737C9FB69EABD539B8D2A249ADLe document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Magnitude of the Root effect in red blood cells and haemoglobin solutions of fishes: a tribute to August Krogh</title><author><name sortKey="Berenbrink, M" sort="Berenbrink, M" uniqKey="Berenbrink M" first="M." last="Berenbrink">M. Berenbrink</name><affiliation><mods:affiliation> Institute of Integrative Biology, University of Liverpool, Liverpool, UK</mods:affiliation></affiliation><affiliation><mods:affiliation> Department of Animal Physiology, Humboldt University Berlin, Berlin, Germany</mods:affiliation></affiliation></author><author><name sortKey="Koldkj R, P" sort="Koldkj R, P" uniqKey="Koldkj R P" first="P." last="Koldkj R">P. Koldkj R</name><affiliation><mods:affiliation> Institute of Integrative Biology, University of Liverpool, Liverpool, UK</mods:affiliation></affiliation></author><author><name sortKey="Hannah Wright, E" sort="Hannah Wright, E" uniqKey="Hannah Wright E" first="E." last="Hannah Wright">E. Hannah Wright</name><affiliation><mods:affiliation> Institute of Integrative Biology, University of Liverpool, Liverpool, UK</mods:affiliation></affiliation></author><author><name sortKey="Kepp, O" sort="Kepp, O" uniqKey="Kepp O" first="O." last="Kepp">O. Kepp</name><affiliation><mods:affiliation> Department of Animal Physiology, Humboldt University Berlin, Berlin, Germany</mods:affiliation></affiliation></author><author><name sortKey="Jose Da Silva, A" sort="Jose Da Silva, A" uniqKey="Jose Da Silva A" first="A." last="José Da Silva">A. José Da Silva</name><affiliation><mods:affiliation> Department of Animal Physiology, Humboldt University Berlin, Berlin, Germany</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:C22E76D81CBA54737C9FB69EABD539B8D2A249AD</idno><date when="2011" year="2011">2011</date><idno type="doi">10.1111/j.1748-1716.2010.02243.x</idno><idno type="url">https://api.istex.fr/document/C22E76D81CBA54737C9FB69EABD539B8D2A249AD/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000820</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000820</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Magnitude of the Root effect in red blood cells and haemoglobin solutions of fishes: a tribute to August Krogh</title><author><name sortKey="Berenbrink, M" sort="Berenbrink, M" uniqKey="Berenbrink M" first="M." last="Berenbrink">M. Berenbrink</name><affiliation><mods:affiliation> Institute of Integrative Biology, University of Liverpool, Liverpool, UK</mods:affiliation></affiliation><affiliation><mods:affiliation> Department of Animal Physiology, Humboldt University Berlin, Berlin, Germany</mods:affiliation></affiliation></author><author><name sortKey="Koldkj R, P" sort="Koldkj R, P" uniqKey="Koldkj R P" first="P." last="Koldkj R">P. Koldkj R</name><affiliation><mods:affiliation> Institute of Integrative Biology, University of Liverpool, Liverpool, UK</mods:affiliation></affiliation></author><author><name sortKey="Hannah Wright, E" sort="Hannah Wright, E" uniqKey="Hannah Wright E" first="E." last="Hannah Wright">E. Hannah Wright</name><affiliation><mods:affiliation> Institute of Integrative Biology, University of Liverpool, Liverpool, UK</mods:affiliation></affiliation></author><author><name sortKey="Kepp, O" sort="Kepp, O" uniqKey="Kepp O" first="O." last="Kepp">O. Kepp</name><affiliation><mods:affiliation> Department of Animal Physiology, Humboldt University Berlin, Berlin, Germany</mods:affiliation></affiliation></author><author><name sortKey="Jose Da Silva, A" sort="Jose Da Silva, A" uniqKey="Jose Da Silva A" first="A." last="José Da Silva">A. José Da Silva</name><affiliation><mods:affiliation> Department of Animal Physiology, Humboldt University Berlin, Berlin, Germany</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Acta Physiologica</title><idno type="ISSN">1748-1708</idno><idno type="eISSN">1748-1716</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2011-07">2011-07</date><biblScope unit="volume">202</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="583">583</biblScope><biblScope unit="page" to="592">592</biblScope></imprint><idno type="ISSN">1748-1708</idno></series><idno type="istex">C22E76D81CBA54737C9FB69EABD539B8D2A249AD</idno><idno type="DOI">10.1111/j.1748-1716.2010.02243.x</idno><idno type="ArticleID">APHA2243</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1748-1708</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Bohr effect</term><term>Root effect</term><term>blood</term><term>haemoglobin</term><term>oxygen transport</term><term>red blood cell</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract">Aim: The ability of high carbon dioxide tensions or low pH to reduce blood oxygen binding even at high oxygen tensions, first observed by August Krogh and Isabella Leitch in 1919 and now known as the Root effect, was studied in red blood cells and haemoglobin solutions of several fish species.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>M. Berenbrink</name><affiliations><json:string>Institute of Integrative Biology, University of Liverpool, Liverpool, UK</json:string><json:string>Department of Animal Physiology, Humboldt University Berlin, Berlin, Germany</json:string></affiliations></json:item><json:item><name>P. Koldkjær</name><affiliations><json:string>Institute of Integrative Biology, University of Liverpool, Liverpool, UK</json:string></affiliations></json:item><json:item><name>E. Hannah Wright</name><affiliations><json:string>Institute of Integrative Biology, University of Liverpool, Liverpool, UK</json:string></affiliations></json:item><json:item><name>O. Kepp</name><affiliations><json:string>Department of Animal Physiology, Humboldt University Berlin, Berlin, Germany</json:string></affiliations></json:item><json:item><name>A. José da Silva</name><affiliations><json:string>Department of Animal Physiology, Humboldt University Berlin, Berlin, Germany</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>blood</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Bohr effect</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>haemoglobin</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>oxygen transport</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>red blood cell</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Root effect</value></json:item></subject><articleId><json:string>APHA2243</json:string></articleId><language><json:string>eng</json:string></language><originalGenre><json:string>article</json:string></originalGenre><qualityIndicators><score>5.612</score><pdfVersion>1.3</pdfVersion><pdfPageSize>595.276 x 793.701 pts</pdfPageSize><refBibsNative>true</refBibsNative><abstractCharCount>295</abstractCharCount><pdfWordCount>6668</pdfWordCount><pdfCharCount>39786</pdfCharCount><pdfPageCount>10</pdfPageCount><abstractWordCount>51</abstractWordCount></qualityIndicators><title>Magnitude of the Root effect in red blood cells and haemoglobin solutions of fishes: a tribute to August Krogh</title><genre><json:string>article</json:string></genre><host><volume>202</volume><publisherId><json:string>APHA</json:string></publisherId><pages><total>10</total><last>592</last><first>583</first></pages><issn><json:string>1748-1708</json:string></issn><issue>3</issue><genre><json:string>journal</json:string></genre><language><json:string>unknown</json:string></language><eissn><json:string>1748-1716</json:string></eissn><title>Acta Physiologica</title><doi><json:string>10.1111/(ISSN)1748-1716</json:string></doi></host><categories><wos><json:string>science</json:string><json:string>physiology</json:string></wos><scienceMetrix><json:string>health sciences</json:string><json:string>biomedical research</json:string><json:string>physiology</json:string></scienceMetrix></categories><publicationDate>2011</publicationDate><copyrightDate>2011</copyrightDate><doi><json:string>10.1111/j.1748-1716.2010.02243.x</json:string></doi><id>C22E76D81CBA54737C9FB69EABD539B8D2A249AD</id><score>0.040436883</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/document/C22E76D81CBA54737C9FB69EABD539B8D2A249AD/fulltext/pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/document/C22E76D81CBA54737C9FB69EABD539B8D2A249AD/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/C22E76D81CBA54737C9FB69EABD539B8D2A249AD/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Magnitude of the Root effect in red blood cells and haemoglobin solutions of fishes: a tribute to August Krogh</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><availability><p>© 2011 The Authors. Acta Physiologica © 2011 Scandinavian Physiological Society</p></availability><date>2011</date></publicationStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Magnitude of the Root effect in red blood cells and haemoglobin solutions of fishes: a tribute to August Krogh</title><author xml:id="author-1"><persName><forename type="first">M.</forename><surname>Berenbrink</surname></persName><affiliation> Institute of Integrative Biology, University of Liverpool, Liverpool, UK</affiliation><affiliation> Department of Animal Physiology, Humboldt University Berlin, Berlin, Germany</affiliation></author><author xml:id="author-2"><persName><forename type="first">P.</forename><surname>Koldkjær</surname></persName><affiliation> Institute of Integrative Biology, University of Liverpool, Liverpool, UK</affiliation></author><author xml:id="author-3"><persName><forename type="first">E.</forename><surname>Hannah Wright</surname></persName><affiliation> Institute of Integrative Biology, University of Liverpool, Liverpool, UK</affiliation></author><author xml:id="author-4"><persName><forename type="first">O.</forename><surname>Kepp</surname></persName><affiliation> Department of Animal Physiology, Humboldt University Berlin, Berlin, Germany</affiliation></author><author xml:id="author-5"><persName><forename type="first">A.</forename><surname>José da Silva</surname></persName><affiliation> Department of Animal Physiology, Humboldt University Berlin, Berlin, Germany</affiliation></author></analytic><monogr><title level="j">Acta Physiologica</title><idno type="pISSN">1748-1708</idno><idno type="eISSN">1748-1716</idno><idno type="DOI">10.1111/(ISSN)1748-1716</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2011-07"></date><biblScope unit="volume">202</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="583">583</biblScope><biblScope unit="page" to="592">592</biblScope></imprint></monogr><idno type="istex">C22E76D81CBA54737C9FB69EABD539B8D2A249AD</idno><idno type="DOI">10.1111/j.1748-1716.2010.02243.x</idno><idno type="ArticleID">APHA2243</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2011</date></creation><langUsage><language ident="en">en</language></langUsage><abstract><p>Aim: The ability of high carbon dioxide tensions or low pH to reduce blood oxygen binding even at high oxygen tensions, first observed by August Krogh and Isabella Leitch in 1919 and now known as the Root effect, was studied in red blood cells and haemoglobin solutions of several fish species.</p></abstract><abstract><p>Methods: Red blood cells in physiological saline were acidified at atmospheric oxygen tension by increasing carbon dioxide tensions and the percentage decrease in oxygen content was used to quantify the Root effect. Haemoglobin was incubated in air‐equilibrated citrate buffers between pH 5 and 7 and the percentage decrease in oxygen saturation relative to pH 8 determined by spectral deconvolution.</p></abstract><abstract><p>Results: The maximal magnitude of the Root effect in citrate‐buffered haemoglobin solutions closely matched the value in blood or red blood cells of 11 vertebrates over a Root effect range between 3 and 80%. Contrary to previous reports, there was no evidence for a significant Root effect in red blood cells or haemoglobin solutions of the wels catfish, but a significant Root effect under both conditions in the Siberian sturgeon.</p></abstract><abstract><p>Conclusions: Under the conditions employed in this study, the maximal Root effect of citrate‐buffered haemoglobin solutions closely resembles the maximal Root effect in red blood cells. This strengthens previous studies on the evolution of the Root effect and its role in oxygen concentration at the retina and swimbladder of a large number of fishes that were based on Root effect measurements in haemoglobin solutions.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>keywords</head><item><term>blood</term></item><item><term>Bohr effect</term></item><item><term>haemoglobin</term></item><item><term>oxygen transport</term></item><item><term>red blood cell</term></item><item><term>Root effect</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2011-07">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/C22E76D81CBA54737C9FB69EABD539B8D2A249AD/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>Blackwell Publishing Ltd</publisherName><publisherLoc>Oxford, UK</publisherLoc></publisherInfo><doi origin="wiley" registered="yes">10.1111/(ISSN)1748-1716</doi><issn type="print">1748-1708</issn><issn type="electronic">1748-1716</issn><idGroup><id type="product" value="APHA"></id><id type="publisherDivision" value="ST"></id></idGroup><titleGroup><title type="main" sort="ACTA PHYSIOLOGICA">Acta Physiologica</title></titleGroup></publicationMeta><publicationMeta level="part" position="07103"><doi origin="wiley">10.1111/apha.2011.202.issue-3</doi><titleGroup><title type="specialIssueTitle">SYMPOSIUM: HOMAGE TO AUGUST KROGH CELEBRATING THE 90TH ANNIVERSARY OF HIS NOBEL PRIZE IN PHYSIOLOGY OR MEDICINE</title></titleGroup><numberingGroup><numbering type="journalVolume" number="202">202</numbering><numbering type="journalIssue">3</numbering></numberingGroup><coverDate startDate="2011-07">July 2011</coverDate></publicationMeta><publicationMeta level="unit" type="article" position="26" status="forIssue"><doi origin="wiley">10.1111/j.1748-1716.2010.02243.x</doi><idGroup><id type="unit" value="APHA2243"></id></idGroup><countGroup><count type="pageTotal" number="10"></count></countGroup><titleGroup><title type="tocHeading1">HAEMOGLOBINS AND OXYGEN EXCHANGE</title></titleGroup><copyright>© 2011 The Authors. Acta Physiologica © 2011 Scandinavian Physiological Society</copyright><eventGroup><event type="xmlConverted" agent="Converter:BPG_TO_WML3G version:2.5 mode:FullText" date="2011-06-10"></event><event type="publishedOnlineAccepted" date="2010-12-29"></event><event type="publishedOnlineEarlyUnpaginated" date="2011-03-01"></event><event type="firstOnline" date="2011-03-01"></event><event type="publishedOnlineFinalForm" date="2011-06-10"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:3.8.8" date="2014-01-04"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-10-14"></event></eventGroup><numberingGroup><numbering type="pageFirst" number="583">583</numbering><numbering type="pageLast" number="592">592</numbering></numberingGroup><correspondenceTo>M. Berenbrink, Institute of Integrative Biology, The University of Liverpool, Crown Street, Liverpool L69 7ZB, UK. E‐mail: <email>michaelb@liv.ac.uk</email>, <email>michael.berenbrink@liverpool.ac.uk</email></correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:APHA.APHA2243.pdf"></link></linkGroup></publicationMeta><contentMeta><unparsedEditorialHistory>Received 28 June 2010, revision requested 31 August 2010, revision received 29 November 2010, accepted 16 December 2010</unparsedEditorialHistory><countGroup><count type="figureTotal" number="6"></count><count type="tableTotal" number="0"></count></countGroup><titleGroup><title type="main">Magnitude of the Root effect in red blood cells and haemoglobin solutions of fishes: a tribute to August Krogh</title><title type="shortAuthors">M Berenbrink <i>et al.</i></title><title type="short"><b>Root effect in fish blood and Hb solutions</b></title></titleGroup><creators><creator creatorRole="author" xml:id="cr1" affiliationRef="#a1 #a2"><personName><givenNames>M.</givenNames><familyName>Berenbrink</familyName></personName></creator><creator creatorRole="author" xml:id="cr2" affiliationRef="#a1"><personName><givenNames>P.</givenNames><familyName>Koldkjær</familyName></personName></creator><creator creatorRole="author" xml:id="cr3" affiliationRef="#a1"><personName><givenNames>E.</givenNames><familyName>Hannah Wright</familyName></personName></creator><creator creatorRole="author" xml:id="cr4" affiliationRef="#a2"><personName><givenNames>O.</givenNames><familyName>Kepp</familyName></personName></creator><creator creatorRole="author" xml:id="cr5" affiliationRef="#a2"><personName><givenNames>A.</givenNames><familyName>José da Silva</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="a1" countryCode="GB"><unparsedAffiliation> Institute of Integrative Biology, University of Liverpool, Liverpool, UK</unparsedAffiliation></affiliation><affiliation xml:id="a2" countryCode="DE"><unparsedAffiliation> Department of Animal Physiology, Humboldt University Berlin, Berlin, Germany</unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en"><keyword xml:id="k1">blood</keyword><keyword xml:id="k2">Bohr effect</keyword><keyword xml:id="k3">haemoglobin</keyword><keyword xml:id="k4">oxygen transport</keyword><keyword xml:id="k5">red blood cell</keyword><keyword xml:id="k6">Root effect</keyword></keywordGroup><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Abstract</title><p><b>Aim: </b> The ability of high carbon dioxide tensions or low pH to reduce blood oxygen binding even at high oxygen tensions, first observed by August Krogh and Isabella Leitch in 1919 and now known as the Root effect, was studied in red blood cells and haemoglobin solutions of several fish species.</p><p><b>Methods: </b> Red blood cells in physiological saline were acidified at atmospheric oxygen tension by increasing carbon dioxide tensions and the percentage decrease in oxygen content was used to quantify the Root effect. Haemoglobin was incubated in air‐equilibrated citrate buffers between pH 5 and 7 and the percentage decrease in oxygen saturation relative to pH 8 determined by spectral deconvolution.</p><p><b>Results: </b> The maximal magnitude of the Root effect in citrate‐buffered haemoglobin solutions closely matched the value in blood or red blood cells of 11 vertebrates over a Root effect range between 3 and 80%. Contrary to previous reports, there was no evidence for a significant Root effect in red blood cells or haemoglobin solutions of the wels catfish, but a significant Root effect under both conditions in the Siberian sturgeon.</p><p><b>Conclusions: </b> Under the conditions employed in this study, the maximal Root effect of citrate‐buffered haemoglobin solutions closely resembles the maximal Root effect in red blood cells. This strengthens previous studies on the evolution of the Root effect and its role in oxygen concentration at the retina and swimbladder of a large number of fishes that were based on Root effect measurements in haemoglobin solutions.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Magnitude of the Root effect in red blood cells and haemoglobin solutions of fishes: a tribute to August Krogh</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>Root effect in fish blood and Hb solutions</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Magnitude of the Root effect in red blood cells and haemoglobin solutions of fishes: a tribute to August Krogh</title></titleInfo><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Berenbrink</namePart><affiliation> Institute of Integrative Biology, University of Liverpool, Liverpool, UK</affiliation><affiliation> Department of Animal Physiology, Humboldt University Berlin, Berlin, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">P.</namePart><namePart type="family">Koldkjær</namePart><affiliation> Institute of Integrative Biology, University of Liverpool, Liverpool, UK</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">E.</namePart><namePart type="family">Hannah Wright</namePart><affiliation> Institute of Integrative Biology, University of Liverpool, Liverpool, UK</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">O.</namePart><namePart type="family">Kepp</namePart><affiliation> Department of Animal Physiology, Humboldt University Berlin, Berlin, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">A.</namePart><namePart type="family">José da Silva</namePart><affiliation> Department of Animal Physiology, Humboldt University Berlin, Berlin, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Blackwell Publishing Ltd</publisher><place><placeTerm type="text">Oxford, UK</placeTerm></place><dateIssued encoding="w3cdtf">2011-07</dateIssued><edition>Received 28 June 2010, revision requested 31 August 2010, revision received 29 November 2010, accepted 16 December 2010</edition><copyrightDate encoding="w3cdtf">2011</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">6</extent></physicalDescription><abstract>Aim: The ability of high carbon dioxide tensions or low pH to reduce blood oxygen binding even at high oxygen tensions, first observed by August Krogh and Isabella Leitch in 1919 and now known as the Root effect, was studied in red blood cells and haemoglobin solutions of several fish species.</abstract><abstract>Methods: Red blood cells in physiological saline were acidified at atmospheric oxygen tension by increasing carbon dioxide tensions and the percentage decrease in oxygen content was used to quantify the Root effect. Haemoglobin was incubated in air‐equilibrated citrate buffers between pH 5 and 7 and the percentage decrease in oxygen saturation relative to pH 8 determined by spectral deconvolution.</abstract><abstract>Results: The maximal magnitude of the Root effect in citrate‐buffered haemoglobin solutions closely matched the value in blood or red blood cells of 11 vertebrates over a Root effect range between 3 and 80%. Contrary to previous reports, there was no evidence for a significant Root effect in red blood cells or haemoglobin solutions of the wels catfish, but a significant Root effect under both conditions in the Siberian sturgeon.</abstract><abstract>Conclusions: Under the conditions employed in this study, the maximal Root effect of citrate‐buffered haemoglobin solutions closely resembles the maximal Root effect in red blood cells. This strengthens previous studies on the evolution of the Root effect and its role in oxygen concentration at the retina and swimbladder of a large number of fishes that were based on Root effect measurements in haemoglobin solutions.</abstract><subject lang="en"><genre>keywords</genre><topic>blood</topic><topic>Bohr effect</topic><topic>haemoglobin</topic><topic>oxygen transport</topic><topic>red blood cell</topic><topic>Root effect</topic></subject><relatedItem type="host"><titleInfo><title>Acta Physiologica</title></titleInfo><genre type="journal">journal</genre><identifier type="ISSN">1748-1708</identifier><identifier type="eISSN">1748-1716</identifier><identifier type="DOI">10.1111/(ISSN)1748-1716</identifier><identifier type="PublisherID">APHA</identifier><part><date>2011</date><detail type="title"><title>SYMPOSIUM: HOMAGE TO AUGUST KROGH CELEBRATING THE 90TH ANNIVERSARY OF HIS NOBEL PRIZE IN PHYSIOLOGY OR MEDICINE</title></detail><detail type="volume"><caption>vol.</caption><number>202</number></detail><detail type="issue"><caption>no.</caption><number>3</number></detail><extent unit="pages"><start>583</start><end>592</end><total>10</total></extent></part></relatedItem><identifier type="istex">C22E76D81CBA54737C9FB69EABD539B8D2A249AD</identifier><identifier type="DOI">10.1111/j.1748-1716.2010.02243.x</identifier><identifier type="ArticleID">APHA2243</identifier><accessCondition type="use and reproduction" contentType="copyright">© 2011 The Authors. Acta Physiologica © 2011 Scandinavian Physiological Society</accessCondition><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>Blackwell Publishing Ltd</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Eau/explor/EsturgeonV1/Data/Istex/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000820 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Corpus/biblio.hfd -nk 000820 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Eau
|area= EsturgeonV1
|flux= Istex
|étape= Corpus
|type= RBID
|clé= ISTEX:C22E76D81CBA54737C9FB69EABD539B8D2A249AD
|texte= Magnitude of the Root effect in red blood cells and haemoglobin solutions of fishes: a tribute to August Krogh
}}
| This area was generated with Dilib version V0.6.27. |  |