Determination of acrosin activity of boar spermatozoa by the clinical method: optimization of the assay and changes during short-term storage of semen
Identifieur interne : 000762 ( Istex/Corpus ); précédent : 000761; suivant : 000763Determination of acrosin activity of boar spermatozoa by the clinical method: optimization of the assay and changes during short-term storage of semen
Auteurs : J. Glogowski ; W. Demianowicz ; B. Piros ; A. CiereszkoSource :
- Theriogenology [ 0093-691X ] ; 1998.
Abstract
A clinical assay to evaluate total acrosin activity developed for human semen has been optimized for use in boar spermatozoa. The main modifications included a decrease of sperm number per assay from 1.0 to 10.0 × 106 to 12.5 to 75.0 × 103 spermatozoa, and the time of incubation from 180 to 60 min. Linearity of response for differing quantities of spermatozoa was maintained. Extensive washing of spermatozoa was necessary to eliminate seminal plasma, the source of acrosin inhibitors. Seminal plasma that was diluted 1000 times inhibited acrosin activity by about 50%. To abolish the inhibitory effect of seminal plasma it was necessary to use 25,000-fold dilution. Total acrosin activity of boar spermatozoa was about 100 times higher than that of human spermatozoa. Acrosin activity of boar spermatozoa in extended semen decreased during 7 d of storage. These results indicate that the clinical assay of acrosin activity can be used for boar spermatozoa to evaluate the quality of boar semen.
Url:
DOI: 10.1016/S0093-691X(98)00191-5
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Demianowicz</name><affiliation><mods:affiliation>Department of Molecular Andrology, Institute of Animal Reproduction and Food Research Polish Academy of Sciences, 10-718 Olsztyn, Poland</mods:affiliation></affiliation></author><author><name sortKey="Piros, B" sort="Piros, B" uniqKey="Piros B" first="B" last="Piros">B. Piros</name><affiliation><mods:affiliation>Department of Molecular Andrology, Institute of Animal Reproduction and Food Research Polish Academy of Sciences, 10-718 Olsztyn, Poland</mods:affiliation></affiliation></author><author><name sortKey="Ciereszko, A" sort="Ciereszko, A" uniqKey="Ciereszko A" first="A" last="Ciereszko">A. 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Ciereszko</name><affiliation><mods:affiliation>Department of Molecular Andrology, Institute of Animal Reproduction and Food Research Polish Academy of Sciences, 10-718 Olsztyn, Poland</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: acieresz@food.irzbz.pan.olsztyn.pl</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Theriogenology</title><title level="j" type="abbrev">THE</title><idno type="ISSN">0093-691X</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1998">1998</date><biblScope unit="volume">50</biblScope><biblScope unit="issue">6</biblScope><biblScope unit="page" from="861">861</biblScope><biblScope unit="page" to="872">872</biblScope></imprint><idno type="ISSN">0093-691X</idno></series><idno type="istex">E05DB40443743B50BF3C4D9643FF0A4E649622B9</idno><idno type="DOI">10.1016/S0093-691X(98)00191-5</idno><idno type="PII">S0093-691X(98)00191-5</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0093-691X</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A clinical assay to evaluate total acrosin activity developed for human semen has been optimized for use in boar spermatozoa. The main modifications included a decrease of sperm number per assay from 1.0 to 10.0 × 106 to 12.5 to 75.0 × 103 spermatozoa, and the time of incubation from 180 to 60 min. Linearity of response for differing quantities of spermatozoa was maintained. Extensive washing of spermatozoa was necessary to eliminate seminal plasma, the source of acrosin inhibitors. Seminal plasma that was diluted 1000 times inhibited acrosin activity by about 50%. To abolish the inhibitory effect of seminal plasma it was necessary to use 25,000-fold dilution. Total acrosin activity of boar spermatozoa was about 100 times higher than that of human spermatozoa. Acrosin activity of boar spermatozoa in extended semen decreased during 7 d of storage. These results indicate that the clinical assay of acrosin activity can be used for boar spermatozoa to evaluate the quality of boar semen.</div></front></TEI><istex><corpusName>elsevier</corpusName><author><json:item><name>J Glogowski</name><affiliations><json:string>Department of Molecular Andrology, Institute of Animal Reproduction and Food Research Polish Academy of Sciences, 10-718 Olsztyn, Poland</json:string></affiliations></json:item><json:item><name>W Demianowicz</name><affiliations><json:string>Department of Molecular Andrology, Institute of Animal Reproduction and Food Research Polish Academy of Sciences, 10-718 Olsztyn, Poland</json:string></affiliations></json:item><json:item><name>B Piros</name><affiliations><json:string>Department of Molecular Andrology, Institute of Animal Reproduction and Food Research Polish Academy of Sciences, 10-718 Olsztyn, Poland</json:string></affiliations></json:item><json:item><name>A Ciereszko</name><affiliations><json:string>Department of Molecular Andrology, Institute of Animal Reproduction and Food Research Polish Academy of Sciences, 10-718 Olsztyn, Poland</json:string><json:string>E-mail: acieresz@food.irzbz.pan.olsztyn.pl</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>boar</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>spermatozoa</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>acrosome</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>sperm motility</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>acrosin</value></json:item></subject><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>A clinical assay to evaluate total acrosin activity developed for human semen has been optimized for use in boar spermatozoa. 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xml:id="author-3"><persName><forename type="first">B</forename><surname>Piros</surname></persName><affiliation>Department of Molecular Andrology, Institute of Animal Reproduction and Food Research Polish Academy of Sciences, 10-718 Olsztyn, Poland</affiliation></author><author xml:id="author-4"><persName><forename type="first">A</forename><surname>Ciereszko</surname></persName><email>acieresz@food.irzbz.pan.olsztyn.pl</email><note type="correspondence"><p>Correspondence: FAX 48-89-535-7421</p></note><affiliation>Department of Molecular Andrology, Institute of Animal Reproduction and Food Research Polish Academy of Sciences, 10-718 Olsztyn, Poland</affiliation></author></analytic><monogr><title level="j">Theriogenology</title><title level="j" type="abbrev">THE</title><idno type="pISSN">0093-691X</idno><idno type="PII">S0093-691X(00)X0372-X</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1998"></date><biblScope unit="volume">50</biblScope><biblScope unit="issue">6</biblScope><biblScope unit="page" from="861">861</biblScope><biblScope unit="page" to="872">872</biblScope></imprint></monogr><idno type="istex">E05DB40443743B50BF3C4D9643FF0A4E649622B9</idno><idno type="DOI">10.1016/S0093-691X(98)00191-5</idno><idno type="PII">S0093-691X(98)00191-5</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1998</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>A clinical assay to evaluate total acrosin activity developed for human semen has been optimized for use in boar spermatozoa. 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These results indicate that the clinical assay of acrosin activity can be used for boar spermatozoa to evaluate the quality of boar semen.</p></abstract><textClass><keywords scheme="keyword"><list><head>Keywords</head><item><term>boar</term></item><item><term>spermatozoa</term></item><item><term>acrosome</term></item><item><term>sperm motility</term></item><item><term>acrosin</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1998">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/E05DB40443743B50BF3C4D9643FF0A4E649622B9/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>THE</jid><aid>98001915</aid><ce:pii>S0093-691X(98)00191-5</ce:pii><ce:doi>10.1016/S0093-691X(98)00191-5</ce:doi><ce:copyright type="unknown" year="1998"></ce:copyright></item-info><head><ce:title>Determination of acrosin activity of boar spermatozoa by the clinical method: optimization of the assay and changes during short-term storage of semen</ce:title><ce:author-group><ce:author><ce:given-name>J</ce:given-name><ce:surname>Glogowski</ce:surname></ce:author><ce:author><ce:given-name>W</ce:given-name><ce:surname>Demianowicz</ce:surname></ce:author><ce:author><ce:given-name>B</ce:given-name><ce:surname>Piros</ce:surname></ce:author><ce:author><ce:given-name>A</ce:given-name><ce:surname>Ciereszko</ce:surname><ce:cross-ref refid="COR1"><ce:sup>1</ce:sup></ce:cross-ref><ce:e-address>acieresz@food.irzbz.pan.olsztyn.pl</ce:e-address></ce:author><ce:affiliation><ce:textfn>Department of Molecular Andrology, Institute of Animal Reproduction and Food Research Polish Academy of Sciences, 10-718 Olsztyn, Poland</ce:textfn></ce:affiliation><ce:correspondence id="COR1"><ce:label>1</ce:label><ce:text>Correspondence: FAX 48-89-535-7421</ce:text></ce:correspondence></ce:author-group><ce:date-received day="28" month="5" year="1998"></ce:date-received><ce:date-accepted day="29" month="7" year="1998"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>A clinical assay to evaluate total acrosin activity developed for human semen has been optimized for use in boar spermatozoa. The main modifications included a decrease of sperm number per assay from 1.0 to 10.0 × 10<ce:sup>6</ce:sup> to 12.5 to 75.0 × 10<ce:sup>3</ce:sup> spermatozoa, and the time of incubation from 180 to 60 min. Linearity of response for differing quantities of spermatozoa was maintained. Extensive washing of spermatozoa was necessary to eliminate seminal plasma, the source of acrosin inhibitors. Seminal plasma that was diluted 1000 times inhibited acrosin activity by about 50%. To abolish the inhibitory effect of seminal plasma it was necessary to use 25,000-fold dilution. Total acrosin activity of boar spermatozoa was about 100 times higher than that of human spermatozoa. Acrosin activity of boar spermatozoa in extended semen decreased during 7 d of storage. These results indicate that the clinical assay of acrosin activity can be used for boar spermatozoa to evaluate the quality of boar semen.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords><ce:section-title>Keywords</ce:section-title><ce:keyword><ce:text>boar</ce:text></ce:keyword><ce:keyword><ce:text>spermatozoa</ce:text></ce:keyword><ce:keyword><ce:text>acrosome</ce:text></ce:keyword><ce:keyword><ce:text>sperm motility</ce:text></ce:keyword><ce:keyword><ce:text>acrosin</ce:text></ce:keyword></ce:keywords></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Determination of acrosin activity of boar spermatozoa by the clinical method: optimization of the assay and changes during short-term storage of semen</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Determination of acrosin activity of boar spermatozoa by the clinical method: optimization of the assay and changes during short-term storage of semen</title></titleInfo><name type="personal"><namePart type="given">J</namePart><namePart type="family">Glogowski</namePart><affiliation>Department of Molecular Andrology, Institute of Animal Reproduction and Food Research Polish Academy of Sciences, 10-718 Olsztyn, Poland</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">W</namePart><namePart type="family">Demianowicz</namePart><affiliation>Department of Molecular Andrology, Institute of Animal Reproduction and Food Research Polish Academy of Sciences, 10-718 Olsztyn, Poland</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">B</namePart><namePart type="family">Piros</namePart><affiliation>Department of Molecular Andrology, Institute of Animal Reproduction and Food Research Polish Academy of Sciences, 10-718 Olsztyn, Poland</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">A</namePart><namePart type="family">Ciereszko</namePart><affiliation>Department of Molecular Andrology, Institute of Animal Reproduction and Food Research Polish Academy of Sciences, 10-718 Olsztyn, Poland</affiliation><affiliation>E-mail: acieresz@food.irzbz.pan.olsztyn.pl</affiliation><description>Correspondence: FAX 48-89-535-7421</description><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article"></genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1998</dateIssued><copyrightDate encoding="w3cdtf">1998</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">A clinical assay to evaluate total acrosin activity developed for human semen has been optimized for use in boar spermatozoa. The main modifications included a decrease of sperm number per assay from 1.0 to 10.0 × 106 to 12.5 to 75.0 × 103 spermatozoa, and the time of incubation from 180 to 60 min. Linearity of response for differing quantities of spermatozoa was maintained. Extensive washing of spermatozoa was necessary to eliminate seminal plasma, the source of acrosin inhibitors. Seminal plasma that was diluted 1000 times inhibited acrosin activity by about 50%. To abolish the inhibitory effect of seminal plasma it was necessary to use 25,000-fold dilution. Total acrosin activity of boar spermatozoa was about 100 times higher than that of human spermatozoa. Acrosin activity of boar spermatozoa in extended semen decreased during 7 d of storage. These results indicate that the clinical assay of acrosin activity can be used for boar spermatozoa to evaluate the quality of boar semen.</abstract><subject><genre>Keywords</genre><topic>boar</topic><topic>spermatozoa</topic><topic>acrosome</topic><topic>sperm motility</topic><topic>acrosin</topic></subject><relatedItem type="host"><titleInfo><title>Theriogenology</title></titleInfo><titleInfo type="abbreviated"><title>THE</title></titleInfo><genre type="journal">journal</genre><originInfo><dateIssued encoding="w3cdtf">19981015</dateIssued></originInfo><identifier type="ISSN">0093-691X</identifier><identifier type="PII">S0093-691X(00)X0372-X</identifier><part><date>19981015</date><detail type="volume"><number>50</number><caption>vol.</caption></detail><detail type="issue"><number>6</number><caption>no.</caption></detail><extent unit="issue pages"><start>821</start><end>979</end></extent><extent unit="pages"><start>861</start><end>872</end></extent></part></relatedItem><identifier type="istex">E05DB40443743B50BF3C4D9643FF0A4E649622B9</identifier><identifier type="DOI">10.1016/S0093-691X(98)00191-5</identifier><identifier type="PII">S0093-691X(98)00191-5</identifier><recordInfo><recordContentSource>ELSEVIER</recordContentSource></recordInfo></mods></metadata></istex></record>Pour manipuler ce document sous Unix (Dilib)
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