Microsatellite development for the endangered Yangtze sturgeon (Acipenser dabryanus Duméril, 1869) using 454 sequencing
Identifieur interne : 000728 ( Istex/Corpus ); précédent : 000727; suivant : 000729Microsatellite development for the endangered Yangtze sturgeon (Acipenser dabryanus Duméril, 1869) using 454 sequencing
Auteurs : Q. Zeng ; H. Ye ; A. Ludwig ; Z. Wang ; Y. Zhang ; Z. PengSource :
- Journal of Applied Ichthyology [ 0175-8659 ] ; 2013-12.
Abstract
The Yangtze sturgeon (Acipenser dabryanus) is an endemic species in China. Using 454 sequencing, eight polymorphic tri‐, tetra‐, penta‐, and hexanucleotide microsatellite loci were isolated in this study. The raw sequence data from a one‐eighth run of 454 sequencings were 38.0 Mbp containing 94 222 reads/sequences. Of 80 microsatellite loci, only eight loci were polymorphic in a population of 30 individuals. The number of alleles per locus ranged from 4 to 14 (mean 7.62), and the observed heterozygosities varied between 0.46 and 0.88 (mean 0.74). Cross amplification was tested in congeneric species Acipenser sturio and Acipenser sinensis. These new microsatellite markers will be useful for further studies on genetic variation, parentage analysis, and conservation management for this critically endangered species.
Url:
DOI: 10.1111/jai.12278
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<front><div type="abstract">The Yangtze sturgeon (Acipenser dabryanus) is an endemic species in China. Using 454 sequencing, eight polymorphic tri‐, tetra‐, penta‐, and hexanucleotide microsatellite loci were isolated in this study. The raw sequence data from a one‐eighth run of 454 sequencings were 38.0 Mbp containing 94 222 reads/sequences. Of 80 microsatellite loci, only eight loci were polymorphic in a population of 30 individuals. The number of alleles per locus ranged from 4 to 14 (mean 7.62), and the observed heterozygosities varied between 0.46 and 0.88 (mean 0.74). Cross amplification was tested in congeneric species Acipenser sturio and Acipenser sinensis. These new microsatellite markers will be useful for further studies on genetic variation, parentage analysis, and conservation management for this critically endangered species.</div>
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<creator affiliationRef="#jai12278-aff-0001" corresponding="yes" creatorRole="author" xml:id="jai12278-cr-0006"><personName><givenNames>Z.</givenNames>
<familyName>Peng</familyName>
</personName>
</creator>
</creators>
<affiliationGroup><affiliation countryCode="CN" type="organization" xml:id="jai12278-aff-0001"><orgDiv>Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education)</orgDiv>
<orgDiv>Key Laboratory of Aquatic Science of Chongqing</orgDiv>
<orgName>Southwest University School of Life Science</orgName>
<address><city>Chongqing</city>
<country>China</country>
</address>
</affiliation>
<affiliation countryCode="CN" type="organization" xml:id="jai12278-aff-0002"><orgName>College of Life Sciences and Food Engineering</orgName>
<orgName>Yibin University</orgName>
<address><city>Yibin</city>
<country>China</country>
</address>
</affiliation>
<affiliation countryCode="DE" type="organization" xml:id="jai12278-aff-0003"><orgDiv>Department of Evolutionary Genetics</orgDiv>
<orgName>Institute for Zoo and Wildlife Research</orgName>
<address><city>Berlin</city>
<country>Germany</country>
</address>
</affiliation>
</affiliationGroup>
<fundingInfo><fundingAgency>National Natural Science Foundation of China</fundingAgency>
<fundingNumber>31272283</fundingNumber>
</fundingInfo>
<fundingInfo><fundingAgency>Science and Technology Research Project of China Three Gorges Corporation</fundingAgency>
<fundingNumber>CT‐12‐08‐01</fundingNumber>
</fundingInfo>
<fundingInfo><fundingAgency>Chongqing Key Laboratory (CSTC)</fundingAgency>
</fundingInfo>
<abstractGroup><abstract type="main" xml:id="jai12278-abs-0001"><title type="main">Summary</title>
<p>The <fc>Y</fc>
angtze sturgeon (<i><fc>A</fc>
cipenser dabryanus</i>
) is an endemic species in <fc>C</fc>
hina. Using 454 sequencing, eight polymorphic tri‐, tetra‐, penta‐, and hexanucleotide microsatellite loci were isolated in this study. The raw sequence data from a one‐eighth run of 454 sequencings were 38.0 <fc>M</fc>
bp containing 94 222 reads/sequences. Of 80 microsatellite loci, only eight loci were polymorphic in a population of 30 individuals. The number of alleles per locus ranged from 4 to 14 (mean 7.62), and the observed heterozygosities varied between 0.46 and 0.88 (mean 0.74). Cross amplification was tested in congeneric species <i><fc>A</fc>
cipenser sturio</i>
and <i><fc>A</fc>
cipenser sinensis</i>
. These new microsatellite markers will be useful for further studies on genetic variation, parentage analysis, and conservation management for this critically endangered species.</p>
</abstract>
</abstractGroup>
</contentMeta>
<noteGroup xml:id="jai12278-ntgp-0001"><note xml:id="jai12278-note-0001">Equal contributors</note>
</noteGroup>
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<mods version="3.6"><titleInfo lang="en"><title>Microsatellite development for the endangered Yangtze sturgeon (Acipenser dabryanus Duméril, 1869) using 454 sequencing</title>
</titleInfo>
<titleInfo type="alternative" contentType="CDATA" lang="en"><title>Microsatellite development for the endangered Yangtze sturgeon (Acipenser dabryanus Duméril, 1869) using 454 sequencing</title>
</titleInfo>
<name type="personal"><namePart type="given">Q.</namePart>
<namePart type="family">Zeng</namePart>
<affiliation>Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, Southwest University School of Life Science, Chongqing, China</affiliation>
<description>Equal contributors</description>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">H.</namePart>
<namePart type="family">Ye</namePart>
<affiliation>Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, Southwest University School of Life Science, Chongqing, China</affiliation>
<affiliation>College of Life Sciences and Food Engineering, Yibin University, Yibin, China</affiliation>
<description>Equal contributors</description>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">A.</namePart>
<namePart type="family">Ludwig</namePart>
<affiliation>Department of Evolutionary Genetics, Institute for Zoo and Wildlife Research, Berlin, Germany</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">Z.</namePart>
<namePart type="family">Wang</namePart>
<affiliation>Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, Southwest University School of Life Science, Chongqing, China</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">Y.</namePart>
<namePart type="family">Zhang</namePart>
<affiliation>Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, Southwest University School of Life Science, Chongqing, China</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">Z.</namePart>
<namePart type="family">Peng</namePart>
<affiliation>Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, Southwest University School of Life Science, Chongqing, China</affiliation>
<affiliation>Zuogang Peng, Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Southwest University School of Life Science, Chongqing 400715, China.E‐mail:</affiliation>
<affiliation>E-mail: pengzuogang@gmail.com</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<typeOfResource>text</typeOfResource>
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<originInfo><publisher>Blackwell Publishing Ltd</publisher>
<dateIssued encoding="w3cdtf">2013-12</dateIssued>
<dateCreated encoding="w3cdtf">2013-06-19</dateCreated>
<dateCaptured encoding="w3cdtf">2013-05-01</dateCaptured>
<dateValid encoding="w3cdtf">2013-05-11</dateValid>
<copyrightDate encoding="w3cdtf">2013</copyrightDate>
</originInfo>
<language><languageTerm type="code" authority="rfc3066">en</languageTerm>
<languageTerm type="code" authority="iso639-2b">eng</languageTerm>
</language>
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</physicalDescription>
<abstract>The Yangtze sturgeon (Acipenser dabryanus) is an endemic species in China. Using 454 sequencing, eight polymorphic tri‐, tetra‐, penta‐, and hexanucleotide microsatellite loci were isolated in this study. The raw sequence data from a one‐eighth run of 454 sequencings were 38.0 Mbp containing 94 222 reads/sequences. Of 80 microsatellite loci, only eight loci were polymorphic in a population of 30 individuals. The number of alleles per locus ranged from 4 to 14 (mean 7.62), and the observed heterozygosities varied between 0.46 and 0.88 (mean 0.74). Cross amplification was tested in congeneric species Acipenser sturio and Acipenser sinensis. These new microsatellite markers will be useful for further studies on genetic variation, parentage analysis, and conservation management for this critically endangered species.</abstract>
<note type="funding">National Natural Science Foundation of China - No. 31272283; </note>
<note type="funding">Science and Technology Research Project of China Three Gorges Corporation - No. CT‐12‐08‐01; </note>
<note type="funding">Chongqing Key Laboratory (CSTC)</note>
<relatedItem type="host"><titleInfo><title>Journal of Applied Ichthyology</title>
</titleInfo>
<titleInfo type="abbreviated"><title>J. Appl. Ichthyol.</title>
</titleInfo>
<genre type="journal">journal</genre>
<subject><genre>article-category</genre>
<topic>Short Communication</topic>
</subject>
<identifier type="ISSN">0175-8659</identifier>
<identifier type="eISSN">1439-0426</identifier>
<identifier type="DOI">10.1111/(ISSN)1439-0426</identifier>
<identifier type="PublisherID">JAI</identifier>
<part><date>2013</date>
<detail type="volume"><caption>vol.</caption>
<number>29</number>
</detail>
<detail type="issue"><caption>no.</caption>
<number>6</number>
</detail>
<extent unit="pages"><start>1219</start>
<end>1221</end>
<total>3</total>
</extent>
</part>
</relatedItem>
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<identifier type="DOI">10.1111/jai.12278</identifier>
<identifier type="ArticleID">JAI12278</identifier>
<accessCondition type="use and reproduction" contentType="copyright">Copyright © 2013 Blackwell Verlag GmbH© 2013 Blackwell Verlag GmbH</accessCondition>
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