Microsatellite development for the endangered Yangtze sturgeon (Acipenser dabryanus Duméril, 1869) using 454 sequencing
Identifieur interne : 000728 ( Istex/Corpus ); précédent : 000727; suivant : 000729Microsatellite development for the endangered Yangtze sturgeon (Acipenser dabryanus Duméril, 1869) using 454 sequencing
Auteurs : Q. Zeng ; H. Ye ; A. Ludwig ; Z. Wang ; Y. Zhang ; Z. PengSource :
- Journal of Applied Ichthyology [ 0175-8659 ] ; 2013-12.
Abstract
The Yangtze sturgeon (Acipenser dabryanus) is an endemic species in China. Using 454 sequencing, eight polymorphic tri‐, tetra‐, penta‐, and hexanucleotide microsatellite loci were isolated in this study. The raw sequence data from a one‐eighth run of 454 sequencings were 38.0 Mbp containing 94 222 reads/sequences. Of 80 microsatellite loci, only eight loci were polymorphic in a population of 30 individuals. The number of alleles per locus ranged from 4 to 14 (mean 7.62), and the observed heterozygosities varied between 0.46 and 0.88 (mean 0.74). Cross amplification was tested in congeneric species Acipenser sturio and Acipenser sinensis. These new microsatellite markers will be useful for further studies on genetic variation, parentage analysis, and conservation management for this critically endangered species.
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DOI: 10.1111/jai.12278
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Ye</name><affiliation><mods:affiliation>Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, Southwest University School of Life Science, Chongqing, China</mods:affiliation></affiliation><affiliation><mods:affiliation>College of Life Sciences and Food Engineering, Yibin University, Yibin, China</mods:affiliation></affiliation></author><author><name sortKey="Ludwig, A" sort="Ludwig, A" uniqKey="Ludwig A" first="A." last="Ludwig">A. Ludwig</name><affiliation><mods:affiliation>Department of Evolutionary Genetics, Institute for Zoo and Wildlife Research, Berlin, Germany</mods:affiliation></affiliation></author><author><name sortKey="Wang, Z" sort="Wang, Z" uniqKey="Wang Z" first="Z." last="Wang">Z. Wang</name><affiliation><mods:affiliation>Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, Southwest University School of Life Science, Chongqing, China</mods:affiliation></affiliation></author><author><name sortKey="Zhang, Y" sort="Zhang, Y" uniqKey="Zhang Y" first="Y." last="Zhang">Y. Zhang</name><affiliation><mods:affiliation>Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, Southwest University School of Life Science, Chongqing, China</mods:affiliation></affiliation></author><author><name sortKey="Peng, Z" sort="Peng, Z" uniqKey="Peng Z" first="Z." last="Peng">Z. Peng</name><affiliation><mods:affiliation>Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, Southwest University School of Life Science, Chongqing, China</mods:affiliation></affiliation><affiliation><mods:affiliation>Zuogang Peng, Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Southwest University School of Life Science, Chongqing 400715, China.E‐mail:</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: pengzuogang@gmail.com</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:33C4D9F37F952FCB180F4D9F4E0F26928311F8CD</idno><date when="2013" year="2013">2013</date><idno type="doi">10.1111/jai.12278</idno><idno type="url">https://api.istex.fr/document/33C4D9F37F952FCB180F4D9F4E0F26928311F8CD/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000728</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000728</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Microsatellite development for the endangered Yangtze sturgeon (Acipenser dabryanus Duméril, 1869) using 454 sequencing</title><author><name sortKey="Zeng, Q" sort="Zeng, Q" uniqKey="Zeng Q" first="Q." last="Zeng">Q. Zeng</name><affiliation><mods:affiliation>Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, Southwest University School of Life Science, Chongqing, China</mods:affiliation></affiliation></author><author><name sortKey="Ye, H" sort="Ye, H" uniqKey="Ye H" first="H." last="Ye">H. Ye</name><affiliation><mods:affiliation>Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, Southwest University School of Life Science, Chongqing, China</mods:affiliation></affiliation><affiliation><mods:affiliation>College of Life Sciences and Food Engineering, Yibin University, Yibin, China</mods:affiliation></affiliation></author><author><name sortKey="Ludwig, A" sort="Ludwig, A" uniqKey="Ludwig A" first="A." last="Ludwig">A. Ludwig</name><affiliation><mods:affiliation>Department of Evolutionary Genetics, Institute for Zoo and Wildlife Research, Berlin, Germany</mods:affiliation></affiliation></author><author><name sortKey="Wang, Z" sort="Wang, Z" uniqKey="Wang Z" first="Z." last="Wang">Z. Wang</name><affiliation><mods:affiliation>Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, Southwest University School of Life Science, Chongqing, China</mods:affiliation></affiliation></author><author><name sortKey="Zhang, Y" sort="Zhang, Y" uniqKey="Zhang Y" first="Y." last="Zhang">Y. Zhang</name><affiliation><mods:affiliation>Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, Southwest University School of Life Science, Chongqing, China</mods:affiliation></affiliation></author><author><name sortKey="Peng, Z" sort="Peng, Z" uniqKey="Peng Z" first="Z." last="Peng">Z. Peng</name><affiliation><mods:affiliation>Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, Southwest University School of Life Science, Chongqing, China</mods:affiliation></affiliation><affiliation><mods:affiliation>Zuogang Peng, Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Southwest University School of Life Science, Chongqing 400715, China.E‐mail:</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: pengzuogang@gmail.com</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Applied Ichthyology</title><title level="j" type="abbrev">J. Appl. Ichthyol.</title><idno type="ISSN">0175-8659</idno><idno type="eISSN">1439-0426</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><date type="published" when="2013-12">2013-12</date><biblScope unit="volume">29</biblScope><biblScope unit="issue">6</biblScope><biblScope unit="page" from="1219">1219</biblScope><biblScope unit="page" to="1221">1221</biblScope></imprint><idno type="ISSN">0175-8659</idno></series><idno type="istex">33C4D9F37F952FCB180F4D9F4E0F26928311F8CD</idno><idno type="DOI">10.1111/jai.12278</idno><idno type="ArticleID">JAI12278</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0175-8659</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract">The Yangtze sturgeon (Acipenser dabryanus) is an endemic species in China. Using 454 sequencing, eight polymorphic tri‐, tetra‐, penta‐, and hexanucleotide microsatellite loci were isolated in this study. The raw sequence data from a one‐eighth run of 454 sequencings were 38.0 Mbp containing 94 222 reads/sequences. Of 80 microsatellite loci, only eight loci were polymorphic in a population of 30 individuals. The number of alleles per locus ranged from 4 to 14 (mean 7.62), and the observed heterozygosities varied between 0.46 and 0.88 (mean 0.74). Cross amplification was tested in congeneric species Acipenser sturio and Acipenser sinensis. These new microsatellite markers will be useful for further studies on genetic variation, parentage analysis, and conservation management for this critically endangered species.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>Q. Zeng</name><affiliations><json:string>Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, Southwest University School of Life Science, Chongqing, China</json:string></affiliations></json:item><json:item><name>H. Ye</name><affiliations><json:string>Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, Southwest University School of Life Science, Chongqing, China</json:string><json:string>College of Life Sciences and Food Engineering, Yibin University, Yibin, China</json:string></affiliations></json:item><json:item><name>A. Ludwig</name><affiliations><json:string>Department of Evolutionary Genetics, Institute for Zoo and Wildlife Research, Berlin, Germany</json:string></affiliations></json:item><json:item><name>Z. Wang</name><affiliations><json:string>Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, Southwest University School of Life Science, Chongqing, China</json:string></affiliations></json:item><json:item><name>Y. Zhang</name><affiliations><json:string>Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, Southwest University School of Life Science, Chongqing, China</json:string></affiliations></json:item><json:item><name>Z. Peng</name><affiliations><json:string>Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, Southwest University School of Life Science, Chongqing, China</json:string><json:string>Zuogang Peng, Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Southwest University School of Life Science, Chongqing 400715, China.E‐mail:</json:string><json:string>E-mail: pengzuogang@gmail.com</json:string></affiliations></json:item></author><articleId><json:string>JAI12278</json:string></articleId><language><json:string>eng</json:string></language><originalGenre><json:string>shortCommunication</json:string></originalGenre><abstract>The Yangtze sturgeon (Acipenser dabryanus) is an endemic species in China. Using 454 sequencing, eight polymorphic tri‐, tetra‐, penta‐, and hexanucleotide microsatellite loci were isolated in this study. The raw sequence data from a one‐eighth run of 454 sequencings were 38.0 Mbp containing 94 222 reads/sequences. Of 80 microsatellite loci, only eight loci were polymorphic in a population of 30 individuals. The number of alleles per locus ranged from 4 to 14 (mean 7.62), and the observed heterozygosities varied between 0.46 and 0.88 (mean 0.74). Cross amplification was tested in congeneric species Acipenser sturio and Acipenser sinensis. These new microsatellite markers will be useful for further studies on genetic variation, parentage analysis, and conservation management for this critically endangered species.</abstract><qualityIndicators><score>5.283</score><pdfVersion>1.7</pdfVersion><pdfPageSize>595.276 x 841.89 pts (A4)</pdfPageSize><refBibsNative>true</refBibsNative><abstractCharCount>824</abstractCharCount><pdfWordCount>1867</pdfWordCount><pdfCharCount>13083</pdfCharCount><pdfPageCount>3</pdfPageCount><abstractWordCount>118</abstractWordCount></qualityIndicators><title>Microsatellite development for the endangered Yangtze sturgeon (Acipenser dabryanus Duméril, 1869) using 454 sequencing</title><genre><json:string>brief-communication</json:string></genre><host><volume>29</volume><publisherId><json:string>JAI</json:string></publisherId><pages><total>3</total><last>1221</last><first>1219</first></pages><issn><json:string>0175-8659</json:string></issn><issue>6</issue><subject><json:item><value>Short Communication</value></json:item></subject><genre><json:string>journal</json:string></genre><language><json:string>unknown</json:string></language><eissn><json:string>1439-0426</json:string></eissn><title>Journal of Applied Ichthyology</title><doi><json:string>10.1111/(ISSN)1439-0426</json:string></doi></host><categories><wos><json:string>science</json:string><json:string>marine & freshwater biology</json:string><json:string>fisheries</json:string></wos><scienceMetrix><json:string>applied sciences</json:string><json:string>agriculture, fisheries & forestry</json:string><json:string>fisheries</json:string></scienceMetrix></categories><publicationDate>2013</publicationDate><copyrightDate>2013</copyrightDate><doi><json:string>10.1111/jai.12278</json:string></doi><id>33C4D9F37F952FCB180F4D9F4E0F26928311F8CD</id><score>0.64020205</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/document/33C4D9F37F952FCB180F4D9F4E0F26928311F8CD/fulltext/pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/document/33C4D9F37F952FCB180F4D9F4E0F26928311F8CD/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/33C4D9F37F952FCB180F4D9F4E0F26928311F8CD/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Microsatellite development for the endangered Yangtze sturgeon (Acipenser dabryanus Duméril, 1869) using 454 sequencing</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>Blackwell Publishing Ltd</publisher><availability><p>Copyright © 2013 Blackwell Verlag GmbH© 2013 Blackwell Verlag GmbH</p></availability><date>2013-06-19</date></publicationStmt><notesStmt><note>National Natural Science Foundation of China - No. 31272283;</note><note>Science and Technology Research Project of China Three Gorges Corporation - No. CT‐12‐08‐01;</note><note>Chongqing Key Laboratory (CSTC)</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Microsatellite development for the endangered Yangtze sturgeon (Acipenser dabryanus Duméril, 1869) using 454 sequencing</title><author xml:id="author-1"><persName><forename type="first">Q.</forename><surname>Zeng</surname></persName><note type="biography">Equal contributors</note><affiliation>Equal contributors</affiliation><affiliation>Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, Southwest University School of Life Science, Chongqing, China</affiliation></author><author xml:id="author-2"><persName><forename type="first">H.</forename><surname>Ye</surname></persName><note type="biography">Equal contributors</note><affiliation>Equal contributors</affiliation><affiliation>Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, Southwest University School of Life Science, Chongqing, China</affiliation><affiliation>College of Life Sciences and Food Engineering, Yibin University, Yibin, China</affiliation></author><author xml:id="author-3"><persName><forename type="first">A.</forename><surname>Ludwig</surname></persName><affiliation>Department of Evolutionary Genetics, Institute for Zoo and Wildlife Research, Berlin, Germany</affiliation></author><author xml:id="author-4"><persName><forename type="first">Z.</forename><surname>Wang</surname></persName><affiliation>Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, Southwest University School of Life Science, Chongqing, China</affiliation></author><author xml:id="author-5"><persName><forename type="first">Y.</forename><surname>Zhang</surname></persName><affiliation>Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, Southwest University School of Life Science, Chongqing, China</affiliation></author><author xml:id="author-6"><persName><forename type="first">Z.</forename><surname>Peng</surname></persName><email>pengzuogang@gmail.com</email><affiliation>Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, Southwest University School of Life Science, Chongqing, China</affiliation><affiliation>Zuogang Peng, Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Southwest University School of Life Science, Chongqing 400715, China.E‐mail:</affiliation></author></analytic><monogr><title level="j">Journal of Applied Ichthyology</title><title level="j" type="abbrev">J. Appl. Ichthyol.</title><idno type="pISSN">0175-8659</idno><idno type="eISSN">1439-0426</idno><idno type="DOI">10.1111/(ISSN)1439-0426</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><date type="published" when="2013-12"></date><biblScope unit="volume">29</biblScope><biblScope unit="issue">6</biblScope><biblScope unit="page" from="1219">1219</biblScope><biblScope unit="page" to="1221">1221</biblScope></imprint></monogr><idno type="istex">33C4D9F37F952FCB180F4D9F4E0F26928311F8CD</idno><idno type="DOI">10.1111/jai.12278</idno><idno type="ArticleID">JAI12278</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2013-06-19</date></creation><langUsage><language ident="en">en</language></langUsage><abstract><p>The Yangtze sturgeon (Acipenser dabryanus) is an endemic species in China. Using 454 sequencing, eight polymorphic tri‐, tetra‐, penta‐, and hexanucleotide microsatellite loci were isolated in this study. The raw sequence data from a one‐eighth run of 454 sequencings were 38.0 Mbp containing 94 222 reads/sequences. Of 80 microsatellite loci, only eight loci were polymorphic in a population of 30 individuals. The number of alleles per locus ranged from 4 to 14 (mean 7.62), and the observed heterozygosities varied between 0.46 and 0.88 (mean 0.74). Cross amplification was tested in congeneric species Acipenser sturio and Acipenser sinensis. These new microsatellite markers will be useful for further studies on genetic variation, parentage analysis, and conservation management for this critically endangered species.</p></abstract><textClass><keywords scheme="Journal Subject"><list><head>article-category</head><item><term>Short Communication</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2013-05-01">Received</change><change when="2013-05-11">Registration</change><change when="2013-06-19">Created</change><change when="2013-12">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/33C4D9F37F952FCB180F4D9F4E0F26928311F8CD/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component type="serialArticle" version="2.0" xml:id="jai12278" xml:lang="en"><header><publicationMeta level="product"><doi origin="wiley" registered="yes">10.1111/(ISSN)1439-0426</doi><issn type="print">0175-8659</issn><issn type="electronic">1439-0426</issn><idGroup><id type="product" value="JAI"></id></idGroup><titleGroup><title sort="JOURNAL OF APPLIED ICHTHYOLOGY" type="main">Journal of Applied Ichthyology</title><title type="short">J. 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Ichthyol.</title></titleGroup></publicationMeta><publicationMeta level="part" position="12106"><doi origin="wiley">10.1111/jai.2013.29.issue-6</doi><copyright ownership="publisher">Copyright © 2013 Blackwell Verlag GmbH</copyright><numberingGroup><numbering type="journalVolume" number="29">29</numbering><numbering type="journalIssue">6</numbering></numberingGroup><coverDate startDate="2013-12">December 2013</coverDate></publicationMeta><publicationMeta level="unit" position="50" status="forIssue" type="shortCommunication"><doi>10.1111/jai.12278</doi><idGroup><id type="unit" value="JAI12278"></id></idGroup><countGroup><count number="3" type="pageTotal"></count></countGroup><titleGroup><title type="articleCategory">Short Communication</title><title type="tocHeading1">Sturgeon Articles</title></titleGroup><copyright ownership="publisher">© 2013 Blackwell Verlag GmbH</copyright><eventGroup><event date="2013-05-01" type="manuscriptReceived"></event><event date="2013-05-11" type="manuscriptAccepted"></event><event agent="SPS" date="2013-06-19" type="xmlCreated"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:3.2.6 mode:FullText" date="2013-11-21"></event><event type="publishedOnlineEarlyUnpaginated" date="2013-07-31"></event><event type="firstOnline" date="2013-07-31"></event><event type="publishedOnlineFinalForm" date="2013-11-21"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.6.4 mode:FullText" date="2015-10-03"></event></eventGroup><numberingGroup><numbering type="pageFirst">1219</numbering><numbering type="pageLast">1221</numbering></numberingGroup><correspondenceTo><lineatedText><line><b>Author's address:</b> Zuogang Peng, Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Southwest University School of Life Science, Chongqing 400715, China.</line><line>E‐mail: <email>pengzuogang@gmail.com</email></line></lineatedText></correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:JAI.JAI12278.pdf"></link></linkGroup></publicationMeta><contentMeta><titleGroup><title type="main">Microsatellite development for the endangered <fc>Y</fc>angtze sturgeon (<i><fc>A</fc>cipenser dabryanus </i><fc>D</fc>uméril, 1869) using 454 sequencing</title><title type="shortAuthors">Q. Zeng et al.</title></titleGroup><creators>By <creator affiliationRef="#jai12278-aff-0001" creatorRole="author" noteRef="#jai12278-note-0001" xml:id="jai12278-cr-0001"><personName><givenNames>Q.</givenNames> <familyName>Zeng</familyName></personName></creator><creator affiliationRef="#jai12278-aff-0001 #jai12278-aff-0002" creatorRole="author" noteRef="#jai12278-note-0001" xml:id="jai12278-cr-0002"><personName><givenNames>H.</givenNames> <familyName>Ye</familyName></personName></creator><creator affiliationRef="#jai12278-aff-0003" creatorRole="author" xml:id="jai12278-cr-0003"><personName><givenNames>A.</givenNames> <familyName>Ludwig</familyName></personName></creator><creator affiliationRef="#jai12278-aff-0001" creatorRole="author" xml:id="jai12278-cr-0004"><personName><givenNames>Z.</givenNames> <familyName>Wang</familyName></personName></creator><creator affiliationRef="#jai12278-aff-0001" creatorRole="author" xml:id="jai12278-cr-0005"><personName><givenNames>Y.</givenNames> <familyName>Zhang</familyName></personName></creator><creator affiliationRef="#jai12278-aff-0001" corresponding="yes" creatorRole="author" xml:id="jai12278-cr-0006"><personName><givenNames>Z.</givenNames> <familyName>Peng</familyName></personName></creator></creators><affiliationGroup><affiliation countryCode="CN" type="organization" xml:id="jai12278-aff-0001"><orgDiv>Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education)</orgDiv> <orgDiv>Key Laboratory of Aquatic Science of Chongqing</orgDiv> <orgName>Southwest University School of Life Science</orgName> <address><city>Chongqing</city> <country>China</country></address></affiliation><affiliation countryCode="CN" type="organization" xml:id="jai12278-aff-0002"><orgName>College of Life Sciences and Food Engineering</orgName> <orgName>Yibin University</orgName> <address><city>Yibin</city> <country>China</country></address></affiliation><affiliation countryCode="DE" type="organization" xml:id="jai12278-aff-0003"><orgDiv>Department of Evolutionary Genetics</orgDiv> <orgName>Institute for Zoo and Wildlife Research</orgName> <address><city>Berlin</city> <country>Germany</country></address></affiliation></affiliationGroup><fundingInfo><fundingAgency>National Natural Science Foundation of China</fundingAgency><fundingNumber>31272283</fundingNumber></fundingInfo><fundingInfo><fundingAgency>Science and Technology Research Project of China Three Gorges Corporation</fundingAgency><fundingNumber>CT‐12‐08‐01</fundingNumber></fundingInfo><fundingInfo><fundingAgency>Chongqing Key Laboratory (CSTC)</fundingAgency></fundingInfo><abstractGroup><abstract type="main" xml:id="jai12278-abs-0001"><title type="main">Summary</title><p>The <fc>Y</fc>angtze sturgeon (<i><fc>A</fc>cipenser dabryanus</i>) is an endemic species in <fc>C</fc>hina. Using 454 sequencing, eight polymorphic tri‐, tetra‐, penta‐, and hexanucleotide microsatellite loci were isolated in this study. The raw sequence data from a one‐eighth run of 454 sequencings were 38.0 <fc>M</fc>bp containing 94 222 reads/sequences. Of 80 microsatellite loci, only eight loci were polymorphic in a population of 30 individuals. The number of alleles per locus ranged from 4 to 14 (mean 7.62), and the observed heterozygosities varied between 0.46 and 0.88 (mean 0.74). Cross amplification was tested in congeneric species <i><fc>A</fc>cipenser sturio</i> and <i><fc>A</fc>cipenser sinensis</i>. These new microsatellite markers will be useful for further studies on genetic variation, parentage analysis, and conservation management for this critically endangered species.</p></abstract></abstractGroup></contentMeta><noteGroup xml:id="jai12278-ntgp-0001"><note xml:id="jai12278-note-0001">Equal contributors</note></noteGroup></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Microsatellite development for the endangered Yangtze sturgeon (Acipenser dabryanus Duméril, 1869) using 454 sequencing</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Microsatellite development for the endangered Yangtze sturgeon (Acipenser dabryanus Duméril, 1869) using 454 sequencing</title></titleInfo><name type="personal"><namePart type="given">Q.</namePart><namePart type="family">Zeng</namePart><affiliation>Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, Southwest University School of Life Science, Chongqing, China</affiliation><description>Equal contributors</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">H.</namePart><namePart type="family">Ye</namePart><affiliation>Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, Southwest University School of Life Science, Chongqing, China</affiliation><affiliation>College of Life Sciences and Food Engineering, Yibin University, Yibin, China</affiliation><description>Equal contributors</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">A.</namePart><namePart type="family">Ludwig</namePart><affiliation>Department of Evolutionary Genetics, Institute for Zoo and Wildlife Research, Berlin, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Z.</namePart><namePart type="family">Wang</namePart><affiliation>Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, Southwest University School of Life Science, Chongqing, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Y.</namePart><namePart type="family">Zhang</namePart><affiliation>Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, Southwest University School of Life Science, Chongqing, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Z.</namePart><namePart type="family">Peng</namePart><affiliation>Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, Southwest University School of Life Science, Chongqing, China</affiliation><affiliation>Zuogang Peng, Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Southwest University School of Life Science, Chongqing 400715, China.E‐mail:</affiliation><affiliation>E-mail: pengzuogang@gmail.com</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="brief-communication" displayLabel="shortCommunication"></genre><originInfo><publisher>Blackwell Publishing Ltd</publisher><dateIssued encoding="w3cdtf">2013-12</dateIssued><dateCreated encoding="w3cdtf">2013-06-19</dateCreated><dateCaptured encoding="w3cdtf">2013-05-01</dateCaptured><dateValid encoding="w3cdtf">2013-05-11</dateValid><copyrightDate encoding="w3cdtf">2013</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract>The Yangtze sturgeon (Acipenser dabryanus) is an endemic species in China. Using 454 sequencing, eight polymorphic tri‐, tetra‐, penta‐, and hexanucleotide microsatellite loci were isolated in this study. The raw sequence data from a one‐eighth run of 454 sequencings were 38.0 Mbp containing 94 222 reads/sequences. Of 80 microsatellite loci, only eight loci were polymorphic in a population of 30 individuals. The number of alleles per locus ranged from 4 to 14 (mean 7.62), and the observed heterozygosities varied between 0.46 and 0.88 (mean 0.74). Cross amplification was tested in congeneric species Acipenser sturio and Acipenser sinensis. These new microsatellite markers will be useful for further studies on genetic variation, parentage analysis, and conservation management for this critically endangered species.</abstract><note type="funding">National Natural Science Foundation of China - No. 31272283; </note><note type="funding">Science and Technology Research Project of China Three Gorges Corporation - No. CT‐12‐08‐01; </note><note type="funding">Chongqing Key Laboratory (CSTC)</note><relatedItem type="host"><titleInfo><title>Journal of Applied Ichthyology</title></titleInfo><titleInfo type="abbreviated"><title>J. Appl. Ichthyol.</title></titleInfo><genre type="journal">journal</genre><subject><genre>article-category</genre><topic>Short Communication</topic></subject><identifier type="ISSN">0175-8659</identifier><identifier type="eISSN">1439-0426</identifier><identifier type="DOI">10.1111/(ISSN)1439-0426</identifier><identifier type="PublisherID">JAI</identifier><part><date>2013</date><detail type="volume"><caption>vol.</caption><number>29</number></detail><detail type="issue"><caption>no.</caption><number>6</number></detail><extent unit="pages"><start>1219</start><end>1221</end><total>3</total></extent></part></relatedItem><identifier type="istex">33C4D9F37F952FCB180F4D9F4E0F26928311F8CD</identifier><identifier type="DOI">10.1111/jai.12278</identifier><identifier type="ArticleID">JAI12278</identifier><accessCondition type="use and reproduction" contentType="copyright">Copyright © 2013 Blackwell Verlag GmbH© 2013 Blackwell Verlag GmbH</accessCondition><recordInfo><recordContentSource>WILEY</recordContentSource></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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